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| 1 | ZmMs30 Encoding a Novel GDSL Lipase Is Essential for Male Fertility and Valuable for Hybrid Breeding in Maize显示文摘Genic male sterility (GMS) is very useful for hybrid vigor utilization and hybrid seed production. Although a large number of GMS genes have been identified in plants, little is known about the roles of GDSL lipase members in anther and pollen development. Here, we report a maize GMS gene, ZmMs30, which encodes a novel type of GDSL lipase with diverged catalytic residues. Enzyme kinetics and activity assays show that ZmMs30 has lipase activity and prefers to substrates with a short carbon chain. ZmMs30 is specifically expressed in maize anthers during stages 7-9. Loss of ZmMs30 function resulted in defective anther cuticle, irregular foot layer of pollen exine, and complete male sterility. Cytological and lipidomics analyses demonstrate that ZmMs30 is crucial for the aliphatic metabolic pathway required for pollen exine formation and anther cuticle development. Furthermore, we found that male sterility caused by loss of ZmMs30 function was stable in various inbred lines with different genetic background, and that it didn't show any negative effect on maize heterosis and production, suggesting that ZmMs30 is valuable for crossbreeding and hybrid seed production. We then developed a new multi-control sterility system using ZmMs30 and its mutant line, and demonstrated it is feasible for generating desirable GMS lines and valu. able for hybrid maize seed production. Taken together, our study sheds new light on the mechanisms of anther and pollen development, and provides a valuable male-sterility system for hybrid breeding maize. | Xueli An Zhenying Dong Youhui Tian Ke Xie Suowei Wu Taotao Zhu Danfeng Zhang Van Zhou Canfang Niu Biao Ma Quancan Hou Jianxi Bao Simiao Zhang Ziwen Li Yanbo Wang Tingwei Yan Xiaojing Sun Yuwen Zhang Jinping Li Xiangyuan Wan | 2019 | Molecular Plant2019，12，3： | 15 |
| 2 | Mammalian target of rapamycin pathway inhibition enhances the effects of 5-aza-dC on suppressing cell proliferation in human gastric cancer cell lines显示文摘The present study aimed to evaluate the relationship between mTOR signaling pathway and DNA methylation in cell survival,cell cycle,gene expression and protein level on human gastric cancer cells. Human gastric cancer cell lines,MKN45 and SGC7901 were treated with 5-aza-dC,rapamycin and/or LY294002.Cell viability was analyzed by MTT.Cell cycle distribution was evaluated by flow cytometry (FCM).The transcription level of PTEN and p27 Kip1 genes was detected by using real-time PCR.Protein expressions were detected by Western blotting.We found that cell viability was moderately reduced when treated with 5-aza-dC alone,but remarkably reduced when mTOR pathway was inhibited together (P<0.01).mTOR inhibition enhances the effects of 5-aza-dC on arresting cell cycle at G2 phase in human gastric cancer cell lines.The expression of PTEN and p27 Kip1 mRNA was remarkably increased in the gastric cancer cells treated with combind drugs(P<0.01).Phosphorylation of Akt,p70S6K and 4E-BP1 were significantly reduced in the cells treated with LY294002 or RAPA(P<0.01),but we failed to find that 5-aza-dC enhance these effects.We suggested that mTOR inhibition could enhance the effects of 5-aza-dC on suppressing cell proliferation and arresting cell cycle in human gastric cancer cell lines, which might be a potential target for tumor therapy. | SUN DanFeng,TIAN XiaoQing,ZHANG YanJie,CHEN YingXuan,LU Rong,WANG Xia &FANG JingYuan Shanghai Jiao-Tong University Sc0hool of Medicine Renji Hospital,Shanghai Institute of Digestive Disease,Shanghai 200001, China | 2008 | Science China(Life Sciences)2008，51，7： | 12 |
| 3 | Normal Structure and Function of Endothecium Chloroplasts Maintained by ZmMs33-Mediated Lipid Biosynthesis in Tapetal Cells Are Critical for Anther Development in Maize显示文摘Genic male sterility(GMS)is critical for heterosis utilization and hybrid seed production.Although GMS mutants and genes have been studied extensively in plants,it has remained unclear whether chloroplast-associated photosynthetic and metabolic activities are involved in the regulation of anther development.In this study,we characterized the function of ZmMs33/ZmGPAT6,which encodes a member of the glycerol-3-phosphate acyltransferase(GPAT)family that catalyzes the first step of the glycerolipid synthetic pathway.We found that normal structure and function of endothecium(En)chloroplasts maintained by ZmMs33-mediated lipid biosynthesis in tapetal cells are crucial for maize anther development.ZmMs33 is expressed mainly in the tapetum at early anther developmental stages and critical for cell proliferation and expansion at late stages.Chloroplasts in En cells of wild-type anthers function as starch storage sites before stage 10 but as photosynthetic factories since stage 10 to enable starch metabolism and carbohydrate supply.Loss of ZmMs33 function inhibits the biosynthesis of glycolipids and phospholipids,which are major components of En chloroplast membranes,and disrupts the development and function of En chloroplasts,resulting in the formation of abnormal En chloroplasts containing numerous starch granules.Further analyses reveal that starch synthesis during the day and starch degradation at night are greatly suppressed in the mutant anthers,leading to carbon starvation and low energy status,as evidenced by low trehalose-6-phosphate content and a reduced ATP/AMP ratio.The energy sensor and inducer of autophagy,SnRK1,was activated to induce early and excessive autophagy,premature PCD,and metabolic reprogramming in tapetal cells,finally arresting the elongation and development of mutant anthers.Taken together,our results not only show that ZmMs33 is required for normal structure and function of En chloroplasts but also reveal that starch metabolism and photosynthetic activities of En chloroplasts at different developmental stages are essential for normal anther development.These findings provide novel insights for understanding how lipid biosynthesis in the tapetum,the structure and function of En chloroplasts,and energy and substance metabolism are coordinated to maintain maize anther development. | Taotao Zhu Ziwen Li Xueli An Yan Long Xiaofeng Xue Ke Xie Biao Ma Danfeng Zhang Yijian Guan Canfang Niu Zhenying Dong Quancan Hou Lina Zhao Suowei Wu Jinping Li Weiwei Jin Xiangyuan Wan | 2020 | Molecular Plant2020，13，11： | 9 |
| 4 | High-Resolution Genome-wide Association Study Identifies Genomic Regions and Candidate Genes for Important Agronomic Traits in Wheat显示文摘Wheat(Triticum aestivum)is a major staple food crop worldwide.Genetic dissection of important agronomic traits is essential for continuous improvement of wheat yield to meet the demand of the world's growing population.We conducted a large-scale genome-wide association study(GWAS)using a panel of 768 wheat cultivars that were genotyped with 327609 single-nucleotide polymorphisms generated by genotyping-by-sequencing and detected 395 quantitative trait loci(QTLs)for 12 traits under 7 environments.Among them,273 QTLs were delimited to≤1.0-Mb intervals and 7 of them are either known genes(Rht-D,Vrn-B1,and Vrn-D1)that have been cloned or known QTLs(TaGA2ox8,APO1,TaSus1-7B,and Rht12)that were previously mapped.Eight putative candidate genes were identified for three QTLs that enhance spike seed setting and grain size using gene expression data and were validated in three bi-parental populations.Protein sequence analysis identified 33 putative wheat orthologs that have high identity with rice genes in QTLs affecting similar traits.Large r^2 values for additive effects observed among the QTLs for most traits indicated that the phenotypes of these identified QTLs were highly predictable.Results from this study demonstrated that significantly increasing GWAS population size and marker density greatly improves detection and identification of candidate genes underlying a QTL,solidifying the foundation for large-scale QTL fine mapping,candidate gene validation,and developing functional markers for genomics-based breeding in wheat. | Yunlong Pang Chunxia Liu Danfeng Wang Paul St.Amand Amy Bernardo Wenhui Li Fang He Linzhi Li Liming Wang Xiufang Yuan Lei Dong Yu Su Huirui Zhang Meng Zhao Yunlong Liangi Hongze Jia Xitong Shen Yue Lu Hongming Jiang Yuye Wu Anfei Li Honggang Wang Lingrang Kong Guihua Bai Shubing Liu | 2020 | Molecular Plant2020，13，9： | 8 |
| 5 | Circulating tumor cells:biology and clinical significance显示文摘Circulating tumor cells(CTCs)are tumor cells that have sloughed off the primary tumor and extravasate into and circulate in the blood.Understanding of the metastatic cascade of CTCs has tremendous potential for the identification of targets against cancer metastasis.Detecting these very rare CTCs among the massive blood cells is challenging.However,emerging technologies for CTCs detection have profoundly contributed to deepening investigation into the biology of CTCs and have facilitated their clinical application.Current technologies for the detection of CTCs are summarized herein,together with their advantages and disadvantages.The detection of CTCs is usually dependent on molecular markers,with the epithelial cell adhesion molecule being the most widely used,although molecular markers vary between different types of cancer.Properties associated with epithelial-tomesenchymal transition and stemness have been identified in CTCs,indicating their increased metastatic capacity.Only a small proportion of CTCs can survive and eventually initiate metastases,suggesting that an interaction and modulation between CTCs and the hostile blood microenvironment is essential for CTC metastasis.Single-cell sequencing of CTCs has been extensively investigated,and has enabled researchers to reveal the genome and transcriptome of CTCs.Herein,we also review the clinical applications of CTCs,especially for monitoring response to cancer treatment and in evaluating prognosis.Hence,CTCs have and will continue to contribute to providing significant insights into metastatic processes and will open new avenues for useful clinical applications. | Danfeng Lin Lesang Shen Meng Luo Kun Zhang Jinfan Li Qi Yang Fangfang Zhu Dan Zhou Shu Zheng Yiding Chen Jiaojiao Zhou | 2021 | Signal Transduction and Targeted Therapy2021，6，12： | 8 |
| 6 | Whole-exome sequencing identifies a novel INS mutation causative of maturity-onset diabetes of the young 10显示文摘 | Jing Yan Feng Jiang Rong Zhang Tongfu Xu Zhou Zhou Wei Ren Danfeng Peng Yong Liu Cheng Hu Weiping Jia | 2017 | Journal of Molecular Cell Biology2017，9，5： | 7 |
| 7 | Construction of heparin-based hydrogel incorporated with Cu_(5.4)O ultrasmall nanozymes for wound healing and inflammation inhibition显示文摘Excessive production of inflammatory chemokines and reactive oxygen species(ROS)can cause a feedback cycle of inflammation response that has a negative effect on cutaneous wound healing.The use of wound-dressing materials that simultaneously absorb chemokines and scavenge ROS constitutes a novel‘weeding and uprooting’treatment strategy for inflammatory conditions.In the present study,a composite hydrogel comprising an amine-functionalized star-shaped polyethylene glycol(starPEG)and heparin for chemokine sequestration as well as Cu_(5.4)O ultrasmall nanozymes for ROS scavenging(Cu_(5.4)O@Hep-PEG)was developed.The material effectively adsorbs the inflammatory chemokines monocyte chemoattractant protein-1 and interleukin-8,decreasing the migratory activity of macrophages and neutrophils.Furthermore,it scavenges the ROS in wound fluids to mitigate oxidative stress,and the sustained release of Cu_(5.4)O promotes angiogenesis.In acute wounds and impaired-healing wounds(diabetic wounds),Cu_(5.4)O@Hep-PEG hydrogels outperform the standard-of-care product Promogram®in terms of inflammation reduction,increased epidermis regeneration,vascularization,and wound closure. | Yuan Peng Danfeng He Xin Ge Yifei Lu Yuanhao Chai Yixin Zhang Zhengwei Mao Gaoxing Luo Jun Deng Yan Zhang | 2021 | Bioactive Materials2021，6，10： | 7 |
| 8 | Discovery of the ancient Ordovician oil-bearing karst cave in Liuhuanggou,North Tarim Basin,and its significance显示文摘A typical ancient karst cave is found in the Yijianfang Formation of Ordovician at Liuhuanggou,North Tarim Basin.With an entrance of 4.8 m wide and 20 m high,the Liuhuanggou ancient karst cave extends to NW 315° and has been filled by sediments,which can be classified into three types according to their nature and origin:clastic sediments(gravels),chemical sediments(palaeotravertine),and mixed sediments.Among them,the Paleotravertine and gravels are most common.The palaeotravertine is laminated type and yellowish brown or black in color due to oil inside.When saturated with oil,the palaeotravertine is black.Under microscope,oil mainly accumulates in the cracks between calcite crystals.The filling period of the Liuhuanggou karst cave can be divided into three stages:mixed filling,chemical filling,and clastic(palaeotravertine breccia) filling.Numerous fractures are distributed on one side of the karst cave.The fractures can be as wide as 40 cm and over 40 m long.Fractures of different periods and sizes constitute a fracture network and are all filled with calcite veins.Oil can be found inside the fractures.In the chemical fillings,sulfur is also found,which can indicate the magma influence on karst caves.The existence of sulfur also confirms that the Liuhunggou karst cave was formed in ancient times rather than recently or nowadays.Different from normal sedimentary limestone,the ancient Liuhuanggou karst cave has a distinct isotope fractionation and the depletion of heavy carbon and oxygen isotope.Controlled by the northwest oriented vertical strike-slip shear fault,the karst cave is actually a dissolved vertical fault belt. | ZHONG JianHua MAO Cui LI Yong LI Yang YUAN XiangChun NIU YongBin CHEN Xin HuANG ZhiJuan SHAO ZhuFu WANG PeiJun LI Jia ZHANG DanFeng | 2012 | Science China Earth Sciences2012，55，9： | 4 |
| 9 | Analysis of the Global Burden of Disease study highlights the trends in death and disability-adjusted life years of leukemia from 1990 to 2017显示文摘Background:The patterns of leukemia burden have dramatically changed in recent years.This study aimed to estimate the global trends of leukemiarelated death and disability-adjusted life-years(DALYs)from 1990 to 2017.Methods:The data was acquired from the latest version of the Global Burden of Disease(GBD)study.Estimated annual percentage changes(EAPCs)were calculated to estimate the trend of age-standardized rate(ASR)of death and DALYs due to leukemia and its main subtypes from 1990 to 2017.Results:Globally,the numbers of death and DALYs due to leukemia were 347.58×10^(3)(95%uncertainty interval[UI]=317.26×10^(3)-364.88×10^(3))and 11975.35×10^(3)(95%UI=10749.15×10^(3)-12793.58×10^(3))in 2017,with a 31.22% and 0.03% increase in absolute numbers from 1990 to 2017,respectively.Both of their ASR showed decreasing trends from 1990 to 2017 with the EAPCs being−1.04(95%confidence interval[CI]=(−1.10-−0.99)and−1.52(95%CI=−1.59-−1.44),respectively.Globally,the most pronounced decreasing trend of death and DALYs occurred in chronic myeloid leukemia with EAPCs of−2.76(95%CI=−2.88-−2.64)and−2.84(95%CI=−2.97-−2.70),respectively,while the trend increased in acute myeloid leukemia.The death and DALYs of leukemia decreased in most areas and countries with high socio-demographic index(SDI)including Bahrain,Finland,and Australia.Conclusions:The disease burden of death and DALYs due to leukemia decreased globally,and for most regions and countries from 1990 to 2017.However,the leukemia burden is still a substantial challenge globally and required adequate and affordable medical resources to improve the survival and quality of life of leukemia patients. | Zejin Ou Danfeng Yu Yuanhao Liang Wenqiao He Yongzhi Li Minyi Zhang Fangfei You Huan He Qing Chen | 2020 | Cancer Communications2020，40，11： | 4 |
| 10 | Zinc finger protein 278, a potential oncogene in human colorectal cancer显示文摘(ZNF278 ) 锌手指蛋白质 278 是在人的纸巾是无所不在地分布式的新奇 Krueppel Cys2-His2-type 锌手指蛋白质。ZNF278 是否与 colorectal 癌症的发展有关,仍然是不清楚的。ZNF278 的 tran-scriptional 水平被即时聚合酶链反应在 colorectal 癌症学习。结果证明那 ZNF278 表情与相应非癌的纸巾相比在 53% colorectal 癌症纸巾被增加。ZNF278 的 Thetranscriptional 下面规定被检测在仅仅三(6%) 与相应非癌症纸巾相比的人的 colorectal 癌症纸巾。没有重要差别在 19 被检测(41%) 一些样品。然而,我们没能发现在抄写和年龄,性别,渗入的度,或肿瘤 colorectal 癌症缩放的 ZNF278 的起来规定之间的一个重要协会。在 colorectal carcinogenesis 学习 ZNF278 的函数,结肠癌房间线 SW1116 是稳定地有到构造的野类型的 ZNF278 plasmid 的 transfected 一个 overexpression 系统,并且短暂地有到构造的 ZNF278 的小介入 RNA 的 transfected 一个 ZNF278 击倒的系统。房间增长与 3- 被估计[4, 5-dimethylthiazol-2-yl ]-2,5-diphenyltetrazolium 溴化物染料和一个房间柜台。结果证明 ZNF278 支持房间生长,并且它的击倒压制房间增长。ZNF278 能是在 colorectal 癌症的潜在的 proto-oncogene。 | Xiaoqing Tian Danfeng Sun Yanjie Zhang Shuliang Zhao Hua Xiong Jingyuan Fang | 2008 | Acta Biochimica et Biophysica Sinica2008，40，4： | 4 |
| 11 | Morphophysiological and molecular evidence supporting the augmentative role of Piriformospora indica in mitigation of salinity in Cucumis melo L.显示文摘Salinity is one of the major limiting factors in plant growth and productivity.Cucumis melo L.is a widely cultivated plant,but its productivity is significantly influenced by the level of salinity in soil.Symbiotic colonization of plants with Piriformospora indica has shown a promotion in plants growth and tolerance against biotic stress.In this study,physiological markers such as ion analysis,antioxidant determination,proline content,electrolyte leakage and chlorophyll measurement were assessed in melon cultivar under two concentrations(100 and 200 mM)of NaCl with and without P.indica inoculation.Results showed that the endophytic inoculation consistently upregulated the level of antioxidants,enhanced plants to antagonize salinity stress.The expression level of an RNA editing factor(SLO2)which is known to participate in mitochondria electron transport chain was analyzed,and its full mRNA sequence was obtained by rapid amplification of cDNA ends(RACE).Under salinity stress,the expression level of SLO2 was increased,enhancing the plant’s capability to adapt to the stress.However,P.indica inoculation further elevated the expression level of SLO2.These findings suggested that the symbiotic association of fungi could help the plants to tolerate the salinity stress. | Danial Hassani Muhammad Khalid Danfeng Huang Yi-Dong Zhang | 2019 | Acta Biochimica et Biophysica Sinica2019，51，3： | 3 |
| 12 | Clinical and microbiological characterization of Clostridium difficile infection in a tertiary care hospital in Shanghai, China显示文摘 | Dong Danfeng Peng Yibing Zhang Lihua Jiang Cen Wang Xuefeng Mao Enqiang | 2014 | Chinese Medical Journal2014，，9： | 3 |
| 13 | A polypropylene melt-blown strategy for the facile and efficient membrane separation of oil-water mixtures显示文摘Porous materials with selective wettability and permeability have significant importance in oil-water separation,but complex fabrication processes are typically required to obtain the desired structures with suitable surface chemistry.In this work,an industrial melt-blown strategy that utilized commercially available polypropylene(PP)was used for the large-scale fabrication of superhydrophobic/superoleophilic membranes with staggered fabric structures.These membranes could readily separate different oils including pump oil and crude oil from various aqueous solutions such as strongly acidic,alkaline,and saline media.In addition,the separation efficiencies of these membranes exceeded 99%,and they could remain functional even after exposure to corrosive media.We anticipate that this work will further the design of membranes and enhance their applicability in oil-water separation,and provide researchers and engineers with a more effective tool for performing challenging separations and mitigating pollution. | Zhenqiang Zhang Danfeng Yu Xiubin Xu Huayi Li Taoyan Mao Cheng Zheng Jianjia Huang Hui Yang Zihan Niu Xu Wu | 2021 | Chinese Journal of Chemical Engineering2021，34，1： | 3 |
| 14 | Modeling Patch Characteristics of Farmland Loss for Site Assessment in Urban Fringe of Beijing, China显示文摘Farmland protection and delimitation in the urban fringe considers not only natural factors but also the spatial characters and site factors. Taking Daxing District, Beijing in China as a case study, this paper used landscape ecology and power-law methods to analyze and evaluate farmland loss during the period of 2004-2007 based on the interpretation results of SPOT5 remote sensing images in 2004 and 2007. At the patch level, we selected four landscape indices, namely patch size, shape index, the nearest neighbor distance between farmland and construction land (including residential land and other construction land), and cropping type, to evaluate the risk of farmland loss and establish a farmland site analysis indicator system. The results showed that patch size and shape index have a significant positive correlation with farmland loss, whereas the distance to construction land has a clear negative correlation with farmland loss. As regards cropping type, fallow farmland is much easier for non-agricultural use than cultivated farmland. The relative transition ratio among vegetable land, fallow farmland and cultivated farmland is 1︰5.6︰1. The patch size of lost farmland follows a power-law distribution, indicating that not only small parcels but also large parcels can be lost. Patch size less than 4 ha or more than 15 ha is in high loss risk, between 4 ha and 10 ha in medium loss risk, and larger than 10 ha and less than 15 ha in low risk. Farmland with a more regular shape has a higher likelihood of loss. Patch shape index less than 2.0 is in high loss risk, between 2.0 and 3.0 in medium loss risk, and larger than 3.0 in low risk. Construction land has a varying impact on farmland loss, the residential land effected distance is 1000 m, and that of the other construction land is 2000 m. This analysis showed the relationships between site factors and farmland loss, and the analysis framework can provide support and reference for farmland protection and delimitation of prime farmland in China. | LIU Xiaona ZHANG Weiwei LI Hong SUN Danfeng | 2013 | Chinese Geographical Science2013，23，3： | 3 |
| 15 | Cytoplasmic and nuclear genome variations of rice hybrids and their parents inform the trajectory and strategy of hybrid rice breeding显示文摘The male sterility(MS)line is a prerequisite for efficient production of hybrid seeds in rice,a self-pollinating species.MS line breeding is pivotal for hybrid rice improvement.Understanding the historical breeding tra-jectory will help to improve hybrid rice breeding strategies.Maternally inherited cytoplasm is an appro-priate tool for phylogenetic reconstruction and pedigree tracing in rice hybrids.In this study,we analyzed the cytoplasmic genomes of 1495 elite hybrid rice varieties and identified five major types of cytoplasm,which correspond to different hybrid production systems.As the cytoplasm donors for hybrids,461 MS lines were also divided into five major types based on cytoplasmic and nuclear genomic architecture.Spe-cific core accessions cooperating with different fertility-associated genes drove the sequence divergence of MS lines.Dozens to hundreds of convergent and divergent selective sweeps spanning several agro-nomic trait-associated genes were identified among different types of MS lines.We further analyzed the cross patterns between different types of MS lines and their corresponding restorers.This study system-atically analyzed the cytoplasmic genomes of rice hybrids revealed their relationships with nuclear ge-nomes of MS lines,and illustrated the trajectory of hybrid rice breeding and the strategies for breeding different types of MS lines providing new insights for future improvement of hybrid rice. | Zhoulin Gu Zhou Zhu Zhen Li Qilin Zhan Qi Feng Congcong Zhou Qiang Zhao Yan Zhao Xiaojian Peng Bingxin Dai Rongrong Sun Yan Li Hengyun Lu Lei Zhang Tao Huang Junyi Gong Danfeng Lv Xuehui Huang Bin Han | 2021 | Molecular Plant2021，14，12： | 2 |
| 16 | Comparison of PM_(2.5) and CO_(2) Concentrations in Large Cities of China during the COVID-19 Lockdown显示文摘Estimating the impacts on PM_(2.5)pollution and CO_(2)emissions by human activities in different urban regions is important for developing efficient policies.In early 2020,China implemented a lockdown policy to contain the spread of COVID-19,resulting in a significant reduction of human activities.This event presents a convenient opportunity to study the impact of human activities in the transportation and industrial sectors on air pollution.Here,we investigate the variations in air quality attributed to the COVID-19 lockdown policy in the megacities of China by combining in-situ environmental and meteorological datasets,the Suomi-NPP/VIIRS and the CO_(2)emissions from the Carbon Monitor project.Our study shows that PM_(2.5)concentrations in the spring of 2020 decreased by 41.87%in the Yangtze River Delta(YRD)and 43.30%in the Pearl River Delta(PRD),respectively,owing to the significant shutdown of traffic and manufacturing industries.However,PM_(2.5)concentrations in the Beijing-Tianjin-Hebei(BTH)region only decreased by 2.01%because the energy and steel industries were not fully paused.In addition,unfavorable weather conditions contributed to further increases in the PM_(2.5)concentration.Furthermore,CO_(2)concentrations were not significantly affected in China during the short-term emission reduction,despite a 19.52%reduction in CO_(2)emissions compared to the same period in 2019.Our results suggest that concerted efforts from different emission sectors and effective long-term emission reduction strategies are necessary to control air pollution and CO_(2)emissions. | Chuwei LIU Zhongwei HUANG Jianping HUANG Chunsheng LIANG Lei DING Xinbo LIAN Xiaoyue LIU Li Zhang Danfeng WANG | 2022 | Advances in Atmospheric Sciences2022，39，6： | 2 |
| 17 | Polymerization of methyl methacrylate catalyzed by mono-/bis-salicylaldiminato nickel(Ⅱ) complexes and methylaluminoxane显示文摘Two types of salicylaldiminato-based nickel complexes,mono-ligated Ni(II)complexes([O-C_(6)H_(4)-o-C(H)=N-Ar]Ni(PPh_(3))(Ph)(5),[O-(3,5-Br_(2))C_(6)H_(2)-o-C(H)=N-Ar]Ni(PPh_(3))(Ph)(6),[O-(3-t-Bu)C_(6)H_(3)-o-C(H)=N-Ar]Ni(PPh_(3))(Ph)(7))and bis-ligated Ni(II)complexes([O-(3,5-Br_(2))C_(6)H_(2)-o-C(H)=N-Ar]_(2)Ni(8),[O-(3,5-Br_(2))C_(6)H_(2)-o-C(H)=N-2-C_(6)H_(4)(PhO)]_(2)Ni(9),Ar=2,6-C_(6)H_(3)(i-Pr)_(2))were synthesized and characterized by Fourier transform infrared spectroscopy(FT-IR),nuclear magnetic resonance(NMR),mass spectrography(MS)and elemental analysis(EA).In the presence of methylaluminoxane(MAO)as cocatalyst,all the nickel complexes exhibited high activities for the polymerization of methyl methacrylate(MMA)and syndiotactic-rich poly(methyl methacrylate)(PMMA)was obtained.The complexes with less bulky substituents on salicylaldiminato framework possessed higher activities,while with the same salicylaldiminato,the mono-ligated nickel complexes showed higher catalytic activity than bis-ligated ones. | Jihong LU Danfeng ZHANG Qian CHEN Buwei YU | 2011 | Frontiers of Chemical Science and Engineering2011，5，1： | 2 |
| 18 | Application of fluorescence in situ hybridization in the detection of bladder transitional-cell carcinoma: A multi-center clinical study based on Chinese population显示文摘Objective:To evaluate the diagnostic value of fluorescence in situ hybridization(FISH)in bladder cancer.Methods:We enrolled healthy volunteers and patients who were clinically suspected to have bladder cancer and conducted FISH tests and cytology examinations from August 2007 to December 2008.Receiver operating characteristic(ROC)curve analysis was performed and the area under curve(AUC)values were calculated for both the FISH and urine cytology tests.Results:A cohort of 988 healthy volunteers was enrolled to establish a reference range for the normal population.A total of 4807 patients with hematuria were prospectively,randomly enrolled for the simultaneous analysis of urine cytology,FISH testing,and a final diagnosis as determined by the pathologic findings of a biopsy or a surgically-excised specimen.Overall,the sensitivity of FISH in detecting transitional-cell carcinoma was 82.7%,while that of cytology was 33.4%(p<0.001).The sensitivity values of FISH for non-muscle invasive and muscle invasive bladder transitional-cell carcinoma were 81.7%and 89.6%,respectively(p=0.004).The sensitivity values of FISH for low and high grade bladder cancer were 82.6%and 90.1%,respectively(p=0.002).Conclusion:FISH is significantly more sensitive than voided urine cytology for detecting bladder cancer in patients evaluated for gross hematuria at all cancer grades and stages.Higher sensitivity using FISH was obtained in high grade and muscle invasive tumors. | Liqun Zhou Kaiwei Yang Xuesong Li Yi Ding Dawei Mu Hanzhong Li Yong Yan Jinyi Li Dongwen Wang Wei Li Yulong Cong Jiangping Gao Kewei Ma Yajun Xiao Sheng Zhang Hongyi Jiang Weilie Hu Qiang Wei Xunbo Jin Zhichen Guan Qingyong Liu Danfeng Xu Xin Gao Yongguang Jiang Weimin Gan Guang Sun Qing Wang Yanhui Liu Jianquan Hou Liping Xie Xishuang Song Fengshuo Jin Jiafu Feng Ming Cai Zhaozhao Liang Jie Zhang Dingwei Ye Lin Qi Lulin Ma Jianzhong Shou Yuping Dai Jianyong Shao Ye Tian Shizhe Hong Tao Xu Chuize Kong Zefeng Kang Yuexin Liu Xun Qu Benkang Shi Shaobin Zheng Yi Lin Shujie Xia Dong Wei Jianbo Wu Weiling Fu Zhiping Wang Jianbo Liang | 2019 | Asian Journal of Urology2019，6，1： | 2 |
| 19 | Bioresponsive nanotherapy for preventing dental caries by inhibiting multispecies cariogenic biofilms显示文摘Early childhood caries(ECC)is a public healthcare concern that greatly reduces the quality of life of young children.As a leading factor of ECC,cariogenic biofilms are composed of acidogenic/aciduric pathogens and extracellular polysaccharides(EPSs),creating an acidic and protected microenvironment.Antimicrobial photodynamic therapy(aPDT)is a noninvasive,painless,and efficient therapeutic approach that is suitable for treating ECC.However,due to the hyperfine structure of cariogenic biofilms,most photosensitizers(PSs)could not access and penetrate deeply in biofilms,which dramatically hamper their efficiency in the clinic.Herein,bioresponsive nanoparticle loaded with chlorin e6(MPP-Ce6)is developed,which largely increases the penetration depth(by over 75%)and retention(by over 100%)of PS in the biofilm compared with free Ce6.Furthermore,MPP-Ce6-mediated aPDT not only kills the bacteria in preformed biofilms but also inhibits multispecies biofilm formation.A rampant caries model is established to mimic ECC in vivo,where the population of cariogenic bacteria is decreased to 10%after MPP-Ce6-mediated aPDT.Importantly,the number and severity of carious lesions are efficiently reduced via Keyes’scoring and micro-CT analysis.This simple but effective strategy can serve as a promising approach for daily oral hygiene in preventing ECC. | Danfeng Liu Xianbin Ma Yaoting Ji Rourong Chen Shuhui Zhou Hantao Yao Zichen Zhang Mengjie Ye Zhigang Xu Minquan Du | 2022 | Bioactive Materials2022，7，8： | 2 |
| 20 | Food Safety Risks and Contributing Factors of Cronobacter spp.显示文摘Cronobacter species are a group of Gram-negative opportunistic pathogens,which cause meningitis,sep-ticemia,and necrotizing enterocolitis in neonates and infants,with neurological sequelae in severe cases.Interest in Cronobacter has increased significantly in recent years due to its high virulence in children.In this review,we summarize the current understanding of the prevalence of Cronobacter species in several important food types.We discuss the response mechanisms enabling persistence in adverse growth con-ditions,as well as its pathogenicity.We emphasize the food safety concerns caused by Cronobacter and subsequent control methods and clinical treatments. | Na Ling Xiuting Jiang Stephen Forsythe Danfeng Zhang Yizhong Shen Yu Ding Juan Wang Jumei Zhang Qingping Wu Yingwang Ye | 2022 | Engineering2022，8，5： | 2 |
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