|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Intestinal alkaline phosphatase in the colonic mucosa of children with inflammatory bowel disease显示文摘AIM:To investigate intestinal alkaline phosphatase(iAP) in the intestinal mucosa of children with inflammatory bowel disease(IBD).METHODS:Colonic biopsy samples were taken from 15 newly diagnosed IBD patients and from 10 healthy controls.In IBD patients,specimens were obtainedboth from inflamed and non-inflamed areas.The iAP mRNA and protein expression was determined by reverse transcription-polymerase chain reaction and Western blotting analysis,respectively.Tissue localization of iAP and Toll-like receptor(TLR) 4 was investigated by immunofluorescent staining.RESULTS:The iAP protein level in the inflamed mucosa of children with Crohn's disease(CD) and ulcerative colitis(UC) was significantly decreased when compared with controls(both P < 0.05).Similarly,we found a significantly decreased level of iAP protein in the inflamed mucosa in CD compared with non-inflamed mucosa in CD(P < 0.05).In addition,the iAP protein level in inflamed colonic mucosa in patients with UC was decreased compared with non-inflamed mucosa in patients with CD(P < 0.05).iAP protein levels in the non-inflamed mucosa of patients with CD were similar to controls.iAP mRNA expression in inflamed colonic mucosa of children with CD and UC was not significantly different from that in non-inflamed colonic mucosa with CD.Expression of iAP mRNA in patients with noninflamed mucosa and in controls were similar.Co-localization of iAP with TLR4 showed intense staining with a dotted-like pattern.iAP was present in the inflamed and non-inflamed mucosa of patients with CD,UC,and in control biopsy specimens,irrespective of whether it was present in the terminal ileum or in the colon.However,the fluorescent signal of TLR4 was more pronounced in the colon compared with the terminal ileum in all groups studied.CONCLUSION:Lower than normal iAP protein levels in inflamed mucosa of IBD patients may indicate a role for iAP in inflammatory lesions in IBD.Based on our results,administration of exogenous iAP enzyme to patients with the active form of IBD may be a therapeutic option. | Kriszta Molnár dám Vannay Beáta Szebeni Nóra Fanni Bánki Erna Sziksz ron Cseh Hajnalka Gyrffy Péter László Lakatos Mária Papp András Arató Gábor Veres | 2012 | World Journal of Gastroenterology2012，18，25： | 5 |
| 2 | Increased duodenal expression of mi R-146a and-155 in pediatric Crohn's disease显示文摘AIM: To evaluate the role of micro RNA(mi R)-146 a,-155 and-122 in the duodenal mucosa of pediatric patients with Crohn's disease(CD) and the effect of transforming growth factor-β(TGF-β) on these mi Rs in duodenal epithelial and fibroblast cells.METHODS: Formalin-fixed, paraffin-embedded biopsies derived from the macroscopically inflamed(CD inflamed: n = 10) and intact(CD intact: n = 10) duodenal mucosa of pediatric CD patients and control children(C: n = 10) were examined. Expression of mi R-146 a,-155 and-122 was determined by realtime polymerase-chain reaction(PCR). The expression of the above mi Rs was investigated in recombinant human TGF-β(1 nmol/L, 24 h) or vehicle treated small intestinal epithelial cells(CCL-241) and primary duodenal fibroblast cells derived from healthy children as well.RESULTS: Expression of mi R-146 a was significantly higher in the inflamed duodenal mucosa compared to the intact duodenal mucosa of children with CD(CD inflamed: 3.21 ± 0.50 vs CD intact: 0.62 ± 0.26, p ≤ 0.01) and to the control group(CD inflamed: 3.21 ± 0.50 vs C: 1.00 ± 0.33, p ≤ 0.05). The expression of mi R-155 was significantly increased in the inflamed region of the duodenum compared to the control group(CD inflamed: 4.87 ± 1.02 vs Control: 1.00 ± 0.40, p ≤ 0.001). The expression of mi R-122 was unchanged in the inflamed or intact mucosa of CD patients compared to controls. TGF-β treatment significantly decreased the expression of mi R-155 in small intestinal epithelial cells(TGF-β: 0.7 ± 0.083 vs Control: 1 ± 0.09, p ≤ 0.05) and also the expression of mi R-146a(TGF-β: 0.67 ± 0.04 vs Control: 1 ± 0.15, p ≤ 0.01) and mi R-155(TGF-β: 0.72 ± 0.09 vs Control: 1 ± 0.06, p ≤ 0.05) in primary duodenal fibroblasts compared to corresponding vehicle treated controls. TGF-β treatment did not influence the expression of mi R-122.CONCLUSION: The elevated expression of mi R-146 a and-155 in the inflamed duodenal mucosa of CD patients suggests the role of these mi Rs in the pathomechanism of inflammatory bowel disease. Antiinflammatory TGF-β plays an important role in the regulation of the expression of these mi Rs. | Dániel Szucs Nóra Judit Béres Réka Rokonay Kriszta Boros Katalin Borka Zoltán Kiss András Arató Attila J Szabó ádám Vannay Erna Sziksz Csaba Bereczki Gábor Veres | 2016 | World Journal of Gastroenterology2016，22，26： | 2 |
| 3 | Droplet bar- coding for single-cell transcriptomics applied to embryonic stem cells显示文摘 | Klein AM Mazutis L Akartuna I Tallapragada N Veres A Li V Peshkin L Weitz DA Kirschner MW | 2015 | Ceil2015，161，： | 1 |
| 4 | Histomorphologic and morphometric changes in minor salivary glands of the rat tongue during 4-nitroquinoline-l-oxide-induced carcinogenesis 显示文摘 | Vered M Daniel N Hirshberg A | 2003 | Oral Oncol2003，39，5： | 1 |
| 5 | 4NQO oral carcinogenesis: ani- mal models, molecular markers and future expectations 显示文摘 | Vered M Yarom N Dayan D | 2005 | Oral Oncol2005，41，4： | 1 |
| 6 | Caspase-mediated cleacage converts llvin from an antiapoptotic to a proapoptotlc factor: implications for drug-resistant melanoma 显示文摘 | BOAZ N YUQOUB A VERED B | 2003 | Cancer Res2003，63，10： | 1 |
| 7 | 4NQO oral carcinogenesis:animal models,molecular markers and future expectations显示文摘 | Vered M Yarom N Dayan D | 2005 | Oral oncol2005，41，： | 1 |
| 8 | Intensive group A streptococcal infections in a large tertiary center: epidemiology, characteristics and outcome 显示文摘 | Ben-Abrahanm R Keller N Vered R | 2002 | Infection2002，30，2： | 1 |
| 9 | Easpase-mediated cleavage converts livin from an antiapoptotic to a proapoptotic factor:implications for drug-resistant melanoma显示文摘 | Boaz N Yaqoub A Vered B | 2003 | Cancer Res2003，63，10： | 1 |
| 10 | 4NQO oral carcinogenesis:animal models,molecular markers and future expectations显示文摘 | Vered M Yarom N Dayan D | | 0，，04： | 1 |
| 11 | ISSLS prize winner:microstructure and mechanical disruption of the lumbar disc annulus:part Ⅱ: how the annulus fails under hydrostatic pressure 显示文摘 | VERES S P ROBERTSON P A BROOM N D | 2008 | Spine (Phila Pa 1976)2008，33，25： | 1 |
| 12 | Efficacy of the non-adenosine analogue A1 adenosine receptor agonist (BR-4935) on cardiovascular function after cardiopulmonary bypass显示文摘 | Veres G Radovits T Otila G Hirschberg K Haider H Krieger N | 2010 | Thorac Cardiovase Surg2010，58，： | 1 |
| 13 | Population size and habitat quality affect genetic diversity and fitness in the clonal herb Cirsiumn dissectum显示文摘 | Vere N d Jongejans E Plowman A | | 0，，： | 1 |
| 14 | caspase- mediated cleavage converts livin from an antiapoptotic to a proapoptotic factor: implications for drug-resistant melanoma 显示文摘 | Boaz N Yaqoub A Vered B | 2003 | Cancer Res2003，63，10： | 1 |
| 15 | 4NQO oral carcinogenesis: animal models, molecular markers and future expectations 显示文摘 | VERED M YAROM N DAYAN D | 2005 | OralOncol2005，41，4： | 1 |
| 16 | caspase-mediated cleavage converts livin from an antiapoptotic to a proapoptotic factor:implications for drug-resistant melanoma显示文摘 | Boaz N Yaqoub A Vered B | 2003 | Cancer Res2003，63，10： | 1 |
| 17 | Novel approach for specific detection of herpes simplex virus type 1 and 2 antibodies and immunoglobulin G and M antibodies显示文摘 | Ohana B Lipson M Vered N | 2000 | CAin Diag Lab Immunol2000，7，6： | 1 |
| 18 | Role of Altered Expression of miR-146a, miR-155, and miR-122 in Pediatric Patients with Inflammatory Bowel Disease显示文摘 | Nóra J. Béres Dolóresz Szabó Dorottya Kocsis Dániel Sz?cs Zoltán Kiss Katalin E. Müller Gábor Lendvai András Kiss András Arató Erna Sziksz ádám Vannay Attila J. Szabó Gábor Veres | 2016 | Inflammatory Bowel Diseases2016，，2： | 1 |
| 19 | Caspase-mediated cleacage converts Livin from an antiapoptotic to a proapoptotic factor: implications for drug-resistant melanoma显示文摘 | Boaz N Yuqoub A Vered B | 2003 | Cancer Res2003，63，10： | 1 |
| 20 | Caspase-mediated cleavage converts livin from an antiapoptotic to a proapoptotic factor:implic ations for drug resistant melanoma显示文摘 | Boaz N Yaqoub A Vered B | 2003 | Cancer Res2003，63，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员