|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A novel gene delivery system targeting cells expressing VEGF receptors显示文摘Two ligand oligopeptides GV1 and GV2 were designed according to the putative binding region of VEGF to its receptors. GV1, GV2 and endosome releasing oligopeptide HA20 were conjugated with poly-L-lysine or protamine and the resulting conjugates could interact with DNA in a noncovalent bond to form a complex. Using pSV2-β-galactosidase as a reporter gene, it has been demonstrated that exogenous gene was transferred into bovine aortic arch-derived endothelial cells (ABAE) andhuman malignant melanoma cell lines (A375) in vitro. In vivo experiments, exogenous gene was transferred into tumor vascular endothelial cells and tumor cells of subcutaneously transplanted human colon cancer LOVO, human malignant melanoma A375 and human hepatoma graft in nude mice. This system could also target gene to intrahepatically transplanted human hepatoma injected via portal vein in nude mice. These results are correlated with theGene delivery system targeting VEGF receptors relevant receptors (flt-1, flk-1/KDR) expression on the targeted cells and tissues. | LI JUN MIN JUN SONG HAN YI HUANG PEI KUN TIAN SHU MIN QU MIN YAO HUI QIU JIANG DA FANG WAN JING CHU LUO CHENG XIAO GU JIAN REN GU( National Labomtory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032,China)(National Laboratory of | 1999 | Cell Research1999，9，1： | 22 |
| 2 | The ATF/CREB site is the key element for transcription of the human RNA methyltransferase like 1 (RNMTL1) gene, a newly discovered 17p13.3 gene显示文摘The human RNA methyltransferase like i gene (RNMTL1) is one of thirteen newly discovered geneswithin a 116 Kb segment of the chromosome 17p13.3 that suffers from a high frequent loss of heterozygosityin human hepatocellular carcinoma in China[1-5]. To understand the molecular mechanisms underlyingtranscription control of the RNMTL1 gene in human cancers, we decline using of the conventional approachwhere the cis-elements bound by the known transcription factors are primary targets, and carried out thesystematic analyses to dissect the promoter structure and identify/characterize the key cis-elements thatare responsible for its strong expression in cell. The molecular approaches applied included 1, the primerextension for mapping of the transcription starts; 2, the transient transfection/reporter assays on a largenumber of deletion and site-specific mutants of the promoter segment for defining the minimal promoterand the crucial elements within; and 3, the electrophoresis mobility shift assay with specific antibodies forreconfirming the nature of the transcription factors and their cognate cis-elements. We have shown that theinteraction of an ATF/CREB element (-38 to -31) and its cognate transcription factors play a predominantrole in the promoter activity of the RNMTL1 gene. The secondary DNA structures of the ATF/CREBelement play a more vital role in the protein-DNA interaction. Finally, we reported a novel mechanismunderlying the YY1 mediated transcription repression, namely, the ATF/CREB dependent transcription-repression by YY1 is executed in absence of its own sequence-specific binding. | JIAN XU JING DE ZHU MIN NI DA FANG WAN JIAN REN GU | 2002 | Cell Research2002，12，4： | 14 |
| 3 | Absolute monocyte and lymphocyte count prognostic score for patients with gastric cancer显示文摘AIM:To measure the prognostic significance of absolute monocyte count/absolute lymphocyte count prognostic score(AMLPS) in patients with gastric cancer.METHODS:We retrospectively examined the combination of absolute monocyte count(AMC) and absolute lymphocyte count(ALC) as prognostic variables in a cohort of 299 gastric cancer patients who underwent surgical resection between 2006 and 2013 and were followed at a single institution.Both AMC and ALC were dichotomized into two groups using cut-off points determined by receiving operator characteristic curve analysis.An AMLPS was generated,which stratified patients into three risk groups:low risk(both low AMC and high ALC),intermediate risk(either high AMC or low ALC),and high risk(both high AMC and low ALC).The primary objective of the study was to validate the impact of AMLPS on both disease-free survival(DFS) and overall survival(OS),and the second objective was to assess the AMLPS as an independent prognostic factor for survival in comparison with known prognostic factors.RESULTS:Using data from the entire cohort,the most discriminative cut-off values of AMC and ALC selected on the receiver operating characteristic curve were 672.4/μL and 1734/μL for DFS and OS.AMLPS risk groups included 158(52.8%) patients in the lowrisk,128(42.8%) in the intermediate-risk,and 13(4.3%) in the high-risk group.With a median followup of 37.2 mo(range:1.7-91.4 mo),five-year DFS rates in the low-,intermediate-,and high-risk groups were 83.4%,78.7%,and 19.8%,respectively.And fiveyear OS rates in the low-,intermediate-,and high-risk groups were 89.3%,81.1%,and 14.4%,respectively.On multivariate analysis performed with patient- and tumor-related factors,we identified AMLPS,age,and pathologic tumor-node-metastasis stage as the most valuable prognostic factors impacting DFS and OS.CONCLUSION:AMLPS identified patients with a poor DFS and OS,and it was independent of age,pathologic stage,and various inflammatory markers. | Wan Kyu Eo Da Wun Jeong Hye Jung Chang Kyu Yeoun Won Sung Il Choi Se Hyun Kim Sung Wook Chun Young Lim Oh Tae Hwa Lee Young Ok Kim Ki Hyung Kim Yong Il Ji Ari Kim Heung Yeol Kim | 2015 | World Journal of Gastroenterology2015，21，9： | 6 |
| 4 | Corrosion Behavior of Commercial Magnetic Refrigerant Gadolinium in Water显示文摘Gadolinium(Gd) used as magnetic refrigerant always works in water environment.However, its poor corrosion resistance is serious impediment against wider application of Gd.In this paper, the corrosion behavior of two types of commercial Gd ( A, B both are 98.9 at.% pure) with the same oxygen content has been studied.The results show that the corrosion rate of A is 3.226 times higher than that of B in deionized water and 6.039 times in tap water.According to SEM, the different corrosion rate is because of the different distribution of impurity in matrix.In addition,NaOH solution was chosen as inhibitor to prevent Gd from being corroded successfully.No pitting corrosion and weight loss were observed for commercial Gd even after immersion for nearly 2000 h in NaOH solution. | Zhang Zeyu Long Yi Wen Da Ye Rongchang Wan Farong | 2004 | Journal of Rare Earths2004，22，z1： | 4 |
| 5 | Evaluation of surgical scrub methods for large animal surgeons 显示文摘 | Wan PY Blackford JT Bemis DA | 1997 | Vet Surg1997，26，5： | 1 |
| 6 | Neuronal differentiation of embryonic midbrain cells by upregulation of peroxisome proliferator-activated receptor-gamma via the JNK-dependent pathway显示文摘 | Ki Sook Park Rhee Da Lee Sun-Kyung Kang Soon Young Han Kui Lae Park Ki Hwa Yang Youn Sook Song Hye Ji Park Yoot Mo Lee Yeo Pyo Yun Ki Wan Oh Dae Joong Kim Young Won Yun Se Jin Hwang Sung Eun Lee Jin Tae Hong | 2004 | Experimental Cell Research2004，，2： | 1 |
| 7 | Effects of Thermal Transfer Paper Performance on Transfer Quality显示文摘 | WAN Da | 2014 | Advanced Materials Research2014，，3： | 1 |
| 8 | Intelligent structured nanocomposite adhesive for bioelectronics and soft robots显示文摘The remarkable functionality of biological systems in detecting and adapting to various environmental conditions has inspired the design of the latest electronics and robots with advanced features.This review focuses on intelligent bio-inspired strategies for developing soft bioelectronics and robotics that can accommodate nanocomposite adhesives and integrate them into biological surfaces.The underlying principles of the material and structural design of nanocomposite adhesives were investigated for practical applications with excellent functionalities,such as soft skin-attachable health care sensors,highly stretchable adhesive electrodes,switchable adhesion,and untethered soft robotics.In addition,we have discussed recent progress in the development of effective fabrication methods for micro/nanostructures for integration into devices,presenting the current challenges and prospects. | Yeon Soo Lee Min-Seok Kim Da Wan Kim Changhyun Pang | 2024 | Nano Research2024，17，2： | 1 |
| 9 | Muscle function and protein metabolism after initiation of eccentric contrac- tion-induced injury显示文摘 | Lowe DA Wan'on GL lngalls CP | 1995 | Journal of Applied Physiology1995，79，4： | 1 |
| 10 | Screen printing process control for coating high throughput titanium dioxide films toward printable mesoscopic perovskite solar cells显示文摘Screen printing technique has been widely applied for the manufacturing of both traditional silicon solar cells and emerging photovoltaics such as dyesensitized solar cells(DSSCs)and perovskite solar cells(PSCs).Particularly,we have developed a printable mesoscopic PSC based on a triple layer scaffold of TiO2/ZrO2/carbon.The deposition of the scaftold is entirely based on screen printing process,which provides a promising prospect for low-cost photovoltaics.However,the optimal thickness of the TiO2 layer for fabricating efficient printable PSCs is much smaller than the typical thickness of screen printed films.Here,we tune the concentration of the pastes and the printing parameters for coating TiO?films,and successfully print TiO2 films with the thickness of 500-550 nm.The correlation between the thickness of the films and printing parameters such as the solid content and viscosity of the pastes,the printing speed and pressure,and the temperature has been investigated.Besides,the edge effect that the edge of the TiO2 films possesses a much larger thickness and printing positional accuracy have been studied.This work will significantly benefit the further development of printable mesoscopic PSCs. | Zhining WAN Mi XU Zhengyang FU Da LI Anyi MEI Yue HU Yaoguang RONG Hongwei HAN | 2019 | Frontiers of Optoelectronics2019，12，4： | 1 |
| 11 | Highwire regulates synaptic growth in drosophila显示文摘 | Aaron DA Fetter RD | 2000 | Neuron2000，26，2： | 1 |
| 12 | Different n-6/n-3 polyunsaturated fatty acid ratios affect postprandial metabolism in normal and hypertriglyceridemic rats显示文摘Postprandial metabolism plays major roles in many pathological conditions.The n-6/n-3 polyunsaturated fatty acid(PUFA)ratio is closely related to various physiological disorders.This study aimed to investigate the effects of high fat meals with different n-6/n-3 PUFA ratios on postprandial metabolism in normal control(NC)and hypertriglyceridemia(HTG)rats.The postprandial response of triglyceride(TG)in HTG groups was higher than that in NC groups after different n-6/n-3 PUFA ratio meals.The HTG groups showed higher postprandial total cholesterol(TC)responses than NC groups after 1:1 and 20:1 ratio meals.The 5:1 n-6/n-3 PUFA ratio elicited lower postprandial responses of tumor necrosis factorα(TNF-α)than 1:1 and 10:1 ratios in HTG groups.The postprandial malondialdehyde(MDA)response was lower after a 5:1 n-6/n-3 PUFA ratio meal than 1:1 and 20:1 ratio meals in HTG groups.The 1:1 ratio resulted in a lower postprandial reactive oxygen species(ROS)level than 5:1 and 10:1 n-6/n-3 PUFA ratios in NC groups.The results showed that a low n-6/n-3 PUFA ratio improved postprandial dysmetabolism induced by a high fat meal in NC and HTG rats.A high n-6/n-3 PUFA ratio increased the difference in postprandial metabolism between NC and HTG rats. | Ligang Yang Chao Yang Zhixiu Song Min Wan Hui Xia Xian Yang Dengfeng Xu Da Pan Hechun Liu Shaokang Wang Guiju Sun | 2023 | Food Science and Human Wellness2023，12，4： | 1 |
| 13 | Remote tissue injury primes hepatocytes for nitric oxide synthesis显示文摘 | Freeswick PD Wan Y Geller DA | 1994 | J Surg Res1994，57，1： | 1 |
| 14 | Design of a novel hybrid s ensor with microelectrode array and LAPS for heavy metal determination using multivariate nonlinear calibration显示文摘 | WAN Hao HA Da ZHANG Wen | 2014 | Sensors and Actuators B2014，192，： | 1 |
| 15 | 显示文摘 | Wan Ying Lu Xiaoling Da Siqin | 2009 | Thermochimica Acta2009，487，12： | 1 |
| 16 | Design of a novel hybrid sensor with microelectrode array and LAPS for heavy metal determination using multivariate nonlinear calibration 显示文摘 | WAN Hao HA Da ZHANG Wen | 2014 | Sensors and Actuators B2014，192，： | 1 |
| 17 | An in vivo model of in situ implantation using pulmonary valved conduit in large animals under off-pump condition显示文摘 | WU Hao XU Zhi-wei LIU Xian-min GONG Da WAN Ju-yi XU Xiu-fang ZHOU Zi-fan LI Wen-bin | 2013 | Chinese Medical Journal2013，，23： | 1 |
| 18 | THE STUDY ON NOVEL MICROELECTRODE ARRAY CHIPS FOR THE DETECTION OF HEAVY METALS IN WATER POLLUTION显示文摘Qualitative and quantitative analysis of trace heavy metals in aqueous environment are rapidly assuming significance along with the rapid development of industry.In this paper,gold microelectrode array(MEA)plated with mercury film was used for simultaneous voltammetric detection of zinc,cadmium,lead and copper ions in water.The electrochemical behavior and the actual surface area of the MEA were investigated by cyclic voltammetry in K_(3)[Fe(CN)_(6)].Electrochemical impedance spectrum(EIS)was utilized to examine the deposition of mercury on the electrode surface.Based on anodic stripping voltammetry,mercury filmAu MEA was applied to the detection of heavy metals in artificial analyte,where good calibrate linearity was obtained for cadmium,lead and copper ions,but with zinc exhibiting poor linearity. | HUI-XIN ZHAO WEI CAI DA HA HAO WAN PING WANG | 2012 | Journal of Innovative Optical Health Sciences2012，5，1： | 1 |
| 19 | Cytokine-induced p38 activation feedback regulates the prolonged activation of AKT cell survival pathway initiated by reactive oxygen species in response to UV irradiation in human keratinocytes 显示文摘 | Maddock DA Chen JP Heo S Chiu C Lai D Souza K Mehta S Wan YS | 2001 | Int J Oncol2001，19，5： | 1 |
| 20 | Acupuncture of different stimulation for the treatment of neck type cervical spondylosis:a randomised controlled trial显示文摘Objective:To observe the difference in clinical efficacy of acupuncture with strong and weak stimulation in treating neck type cervical spondylosis.Methods:64 patients with neck type cervical spondylosis were randomly divided into strong stimulation group and weak stimulation group,with 32 cases in each group.The acupoints selected are Jingjiaji 5-7(cervical acupoints EX-B2 C5-C7),Tianzhu(BL10)on both sides,Houxi(SI3)on both sides,Hegu(LI4)on both sides,Geshu(BL17)on both sides,and Ashi.In the strong stimulation group,deep needling with 0.25 mm×40 mm milli-needle was performed,with a mild reinforcing-reducing method.The twisting angle was 90°-180°,the frequency was 60-90 times/min,and the needles were applied for 1 min at each point.Keep the needle for 30 minutes,twisting was performed every 10 min during the retention period.Change hands once.In the weak stimulation group,shallow acupuncture with a 0.18 mm×40 mm milli-needle was performed,with a mild reinforcing-reducing method,with a twisting angle of 60°-90°,a frequency of 30-60 times/min,and acupuncture for 10 seconds at each acupoint.Keep the needle for 30 minutes,and no needle during the retention period.Patients in both groups were treated once every 1 to 2 days,3 times a week for 2 weeks,and followed up for 1 month after all treatments.The two groups of patients were compared before and after treatment and during follow-up.The McGill pain questionnaire score and the cervical spine dysfunction index score were compared to determine the clinical efficacy.Statistical analysis was performed using SPSS 22.0 statistical software.Results:1 case drop-out in the strong stimulation group and 1 case in the weak stimulation group.The total effective rate of the strong stimulation group was 90.32%,the total effective rate of the weak stimulation group was 83.87%,and the total effective rate of the strong stimulation group was higher than that of the weak stimulation group(P<0.05).The total scores of the short-form McGill pain questionnaire,the VAS score,and the cervical spine dysfunction index scores of the two groups of patients were significantly lower than those of this group before treatment,and the difference was statistically significant(P<0.05).The total scores of the short-form McGill pain questionnaire,visual analogue scale score,and cervical spine dysfunction index scores of the strong stimulation group after treatment and follow-up were significantly lower than those of the weak stimulation group(P<0.05).Conclusion:Acupuncture with strong and weak stimulation can effectively treat patients with cervical spondylosis,and deep acupuncture with filiform needles and strong stimulation have better effect. | Da Li Chun-Yan Chen Wei Zhang Jia-Yu Huang Ren-Hong Wan | 2021 | TMR Non-Drug Therapy2021，4，3： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员