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| 1 | Erythrocyte membrane-camouflaged carrier-free nanoassembly of FRET photosensitizer pairs with high therapeutic efficiency and high security for programmed cancer synergistic phototherapy显示文摘Phototherapy has been intensively investigated as a non-invasive cancer treatment option.However,its clinical translation is still impeded by unsatisfactory therapeutic efficacy and severe phototoxicity.To achieve high therapeutic efficiency and high security,a nanoassembly of Forster Resonance Energy Transfer(FRET)photosensitizer pairs is developed on basis of dual-mode photosensitizer co-loading and photocaging strategy.For proof-of-concept,an erythrocyte-camouflaged FRET pair co-assembly of chlorine e6(Ce6,FRET donor)and 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide(DiR,FRET acceptor)is investigated for breast cancer treatment.Notably,Ce6 in the nanoassemby is quenched by DiR and could be unlocked for photodynamic therapy(PDT)only when DiR is photobleached by 808-nm laser.As a result,Ce6-caused phototoxicity could be well controlled.Under cascaded laser irradiation(808-660 nm),tumor-localizing temperature rise following laser irradiation on DiR not only induces tumor cell apoptosis but also facilitates the tumor penetration of NPs,relieves tumor hypoxia,and promotes the PDT efficacy of Ce6.Such FRET pair-based nanoassembly provides a new strategy for developing multimodal phototherapy nanomedicines with high efficiency and good security. | Xuanbo Zhang Jianchen Xiong Kaiyuan Wang Han Yu Bingjun Sun Hao Ye Zhiqiang Zhao Ning Wang Yuequan Wang Shenwu Zhang Wutong Zhao Haotian Zhang Zhonggui He Cong Luo Jin Sun | 2021 | Bioactive Materials2021，6，8： | 4 |
| 2 | A Secure and Privacy-Preserving Watermark Based Medical Image Sharing Method显示文摘The patients'medical image data are one of the most important data in e-health.Medical image data usually play a crucial role in disease diagnosis and implicate unpredictable potential values for improving diagnostic methods and adjusting diagnostic resuIts.To exploit their incredible potential values,medical images need to be shared among different hospitals,medical institutions and insurance companies and others.Eut how to securely and effectively share these medical image data becomes a challenging problem.In this paper,we proposed to combine encryption and digital watermark t echnology to achieve a secure and privacy-preserving medical image sharing met hod.The QR code image of the concatenation of authoritative diagnosis results and the hash of the original medical image is genera ted as the watermark image.The Discrete cosine transform(DCT)and Inverse DCT(IDCT)algorithms are utilized to embed the watermark image.As the watermarked medical images are desensitized,they are stored to a smart contract based blockchain,such as Ethereum,to achieve secure and fair sharing bet ween data owners and users.The experimen tai results show that the proposed method can resist several attacks meanwhile it is efficient in medical image sharing. | CHEN Lanxiang BAI Wutong YAO Zhiqiang | 2020 | Chinese Journal of Electronics2020，29，5： | 3 |
| 3 | Expression,Purification and Identification of Recombinant Mouse Interleukin 21 Protein in E.coli显示文摘Interleukin 21 (IL-21) is a novel typeⅠcytokine that is significantly homologous to IL-2, IL-4 and IL-15. Its receptor complex containsγc chain which is also a component of receptors for IL-2, IL-4, IL-7, IL-9 and IL-15, so there may be overlapping or relevancies in their biological functions. IL-21 is capable of co-stimulating mature T cells, B cells, NK cells, and of stimulating CD16 expression on the surface of NK cells to induce ADCC in innate immune response. It can also strengthen the anti-tumor effect of the cellular immunity, especially via enhancing the activities of NK and antigen specific CTL cells. Thus, IL-21 is a potential useful therapeutic molecule for immunotherapy of malignancies, by eliciting innate and adaptive anti-tumor immune responses in tumor-bearing hosts. In order to study the biological functions of IL-21, we constructed a mIL-21 prokaryotic expression plasmid and expressed the recombinant mIL-21 protein in E. coli in present study. The recombinant plasmid pET28a/mIL-21 with a carboxyl terminal His-tag was subcloned from the pcDNA3.1/mIL-21 and expressed in E. coli. The induced protein was detected by SDS-PAGE, and identified by Western-blot assay with anti-mIL-21 antibody. The recombinant protein was purified via Ni+ affinity chromatography, and renatured with GSH/GSSG system. Our mouse T cell proliferation experiment showed that the recombinant mIL-21 protein could enhance the mouse T cell proliferation either by itself alone or in the presence of Con A. | Weiguo Tang Guobing Chen Qing Gu Jing Pan Wutong Wu | 2006 | Cellular & Molecular Immunology2006，3，4： | 2 |
| 4 | Gas-initiation under UV and liquid-grafting polymerization on the surface of polysulfone hollow fiber ultrafiltration membrane by dynamic method显示文摘Using the inner-surface of polysulfone hollow fiber ultrafiltration membranes as grafted layer, the method of gas-initiation and liquid-polymerization has been studied, which aimed to adjust the diameter of the pores in the membranes. The degree of polymerization varied with the changes of the parameters, such as irradiation time, monomer concentration, temperature and time of polymerization and so on. The results indicated that using benzophenone(BP) which is in a gaseous condition as photo-initiator, acrylamide as graft monomer, the polyacrylamide chain was grafted on the surface of membranes. After the surface membrane being modified, the water flux and retention altered,and thus it can be seen that the diameter of the pores in the membrane was altered. These experiments contribute to finding a new way to produce the hollow fiber membrane with the small pore size and are extraordinarily worth developing and studying. | SHENYing-jie WUGuang-xia XUShu-guang ZHONGHui ZHANGShao-lai ZHANGXue-zhu ZHAOXian-hong WUTong ZHANGWei-jun | 2005 | Journal of Environmental Sciences2005，17，3： | 2 |
| 5 | Immunological features and efficacy of the reconstructed epitope vaccine CtUBE against Helicobacter pylori infection in BALB/c mice model显示文摘 | Le Guo Kunmei Liu Wenfeng Zhao Xiaokang Li Tong Li Feng Tang Rui Zhang Wutong Wu Tao Xi | 2013 | Applied Microbiology and Biotechnology2013，，： | 2 |
| 6 | Protective Effect of Recombinant Hirudin Variant III against Galactose-Mediated Rat Lens Epithelial Cell Damage显示文摘 | Yu Ou Gaoyong Liao Zhijun Yuan Wutong Wu | 2012 | Current Eye Research2012，，3： | 2 |
| 7 | Efficient expression and secretion of recombinant hirudin III in E. coli using the l -asparaginase II signal sequence显示文摘 | Shuhua Tan Wutong Wu Jingjing Liu Yi Kong Yinghui Pu Riying Yuan | 2002 | Protein Expression and Purification2002，，3： | 1 |
| 8 | Anti-diabetic effect of an α-glucan from fruit body of maitake (Grifola frondosa) on KK-Ay mice显示文摘 | LEI Hong MA Xun WU Wutong | 2007 | J Pharm Pharmcol2007，59，4： | 1 |
| 9 | Oral vaccination with liposome-encapsulated recombinant fusion peptide of urease B epitope and cholera toxin B subunit affords prophylactic and therapeutic effects against H. pylori infection in BALB/c mice显示文摘 | Wenfeng Zhao Wutong Wu Xudong Xu | 2007 | Vaccine2007，，44： | 1 |
| 10 | Anti-diabetic efect of analpha- glucan from fruit body of maitake (Grifola frondosa) on KK- Ay mice显示文摘 | Hong L Xun M Wutong W | 2007 | Pharm Pharmacol2007，59，4： | 1 |
| 11 | Enzymatic synthesis of L-Tryptophan from L-Cysteine andindole显示文摘 | Wei Pinghe Wu Wutong | 1999 | Journal of Chinese Pharmaceutical Science1999，8，4： | 1 |
| 12 | Purification and characterization of Cytotoxins from Agkistrodon acutus venom and their anticancer activity显示文摘 | Zhang Liang Li Hong Wu Wutong | 2004 | J Chin Pharm Sci2004，13，2： | 1 |
| 13 | Anti-diabetic effect of an alpha-glucan from fruit body of maitake(grifola fron-dosa)on KK-Ay mice显示文摘 | Hong L Xun M Wutong W | 2007 | Pharm Pharmacol2007，59，4： | 1 |
| 14 | The protective effect of MT-α-glucan against streptozotocin (STZ)-induced NIT-1 pancreatic β-cell damage显示文摘 | Lei Hong Wang Qin Guo Shuzhen Han Juncheng Sun Hanju Zhang Xiaoxiang Wu Wutong | 2013 | Carbohydrate Polymers2013，，2： | 1 |
| 15 | Purification and characterization of Cytotoxins from A gkistrodon acutus venom and their anticancer activity 显示文摘 | Zhang Liang Li Hong Wu Wutong | 2004 | J Chin Pharm Sci2004，13，2： | 1 |
| 16 | A versatile AIE fluorogen with selective reactivity to primary amines for monitoring amination,protein labeling,and mitochondrial staining显示文摘Specific bioconjugation for native primary amines is highly valuable for both chemistry and biomedical research.Despite all the efforts,scientists lack a proper strategy to achieve high selectivity for primary amines,not to mention the requirement of fast response in real applications.Herein,we report a chromone-based aggregation-induced emission(AIE)fluorogen called CMVMN as a self-reporting bioconjugation reagent for selective primary amine identification,and its applications for monitoring bioprocesses of amination and protein labeling.CMVMN is AIE-active and capable of solid-state sensing.Thus,its electrospun films are manufactured for visualization of amine diffusion and leakage process.CMVMN also shows good biocompatibility and potential mitochondria-staining ability,which provides new insight for organelle-staining probe design.Combined with its facile synthesis and good reversibility,CMVMN would not only show wide potential applications in biology,but also offer new possibilities for molecular engineering. | Xinyuan He Huilin Xie Lianrui Hu Pengchao Liu Changhuo Xu Wei He Wutong Du Siwei Zhang Hao Xing Xinyue Liu Hojeong Park Tsz Shing Cheung Min-Hui Li Ryan T.K.Kwok Jacky W.Y.Lam Jian Lu Ben Zhong Tang | 2023 | Aggregate2023，4，1： | 0 |
| 17 | Photocatalytic Degradation of 4-Chlorophenol by Gd-Doped β-Bi2O3 Under Visible Light Irradiation显示文摘Chlorophenols are known as persistent organic pollutants.Therefore,research on the removal of chlorophenols has attracted widespread attention.Hereto,the photocatalytic degradation of 4-chlorophenol by Gd-doped β-Bi2O3 under visible light irradiation was studied.The results showed that Gd-doped β-Bi2O3 materials are efficient catalysts for the photocatalytic degradation of chlorophenols,and 2%(atomic traction)Gd-doped β-Bi2O3 exhibits the highest photocatalytic activity for 4-chlorophenol degradation,because doping an appropriate amount of Gd^3+ions can effectively reduce the recombination rate of the photogenerated e^-/h^+pairs and then enhance the photocatalytic performance.When the reaction was carried out at 25 ℃ for 6 h using the 2% Gd-doped/β-Bi2O3 micro/nano materials of 200 mg and at air flow rate of 40 mL/min,the degradation rate of 4-chlorophenol reached 92.3%.Additionally based on the analysis of the products,it was speculated that the dominant photocatalytic degradation mechanism of 4-chlorophenol by Gd-doped β-Bi2O3 under visible light irradiation is an oxidative process involving an attack by the hydroxyl radical. | LIN Shijing DU Wutong TONG Laga JI Tao JIAO Xinxin | 2019 | Chemical Research in Chinese Universities2019，35，1： | 0 |
| 18 | Gas sensing selectivity of oxygen-regulated SnO2 films with different microstructure and texture显示文摘The selectivity of gas sensing materials is increasingly important for their applications. The oxygenregulated SnO2 films with(110) and(101) preferred orientation were obtained through magnetron sputtering, followed by annealing treatment. Their micro-structure, surface morphology and gas response were investigated by advanced structural characterization and property measurement. The results showed that the as-prepared(110)-oriented SnO2 film was oxygen-rich and had more adsorption sites while the as-prepared(101)-oriented SnO2 film was oxygen-poor and more sensitive to de-oxidation.H2 gas sensitivity, response speed, selectivity between H2 and CO of the(110)-orientated SnO2 film was superior to that of the(101)-orientated SnO2 film. After treated at high temperature and high vacuum,the reduction of gas-sensing properties of the annealed(110) SnO2 film was much more than that of the annealed(101) SnO2 film. The lattice oxygen was responsible for the difference in gas-sensing response between(110) and(101)-oriented SnO2 films under oxygen regulation. This work indicated the gassensing selectivity of the different crystal planes in SnO2 film, providing a significant reference for design and extension of the related materials. | Ruiwu Li Yanwen Zhou Maolin Sun Zhen Gong Yuanyuan Guo Xitao Yin Fayu Wu Wutong Ding | 2019 | Journal of Materials Science & Technology2019，35，10： | 0 |
| 19 | Coordination-driven self-assembly of palladium(Ⅱ)-based metallacalixarenes as anion receptors using flexible pyridine-bridged diimidazole ligands显示文摘Two types of palladiu m(Ⅱ)-based metallacalixarenes[ML]^(2+)and[ML_(2)]^(2+)have been synthesized through coordination-driven self-assembly from a series of flexible pyridine-bridged diimidazole ligands[2,6-bis((1 H-imidazol-1-yl)methyl)pyridine(L^(1)),2,6-bis((1 H-benzo[d]imidazol-1-yl)methyl)pyridine(L^(2)),2,6-bis((1 H-naphtho[2,3-d]imidazol-1-yl)methyl)pyridine(L^(3))],with palladium(II)-based building blocks[Pd(BF_(4))_(2)(M^(1)-BF_4)and(tmeda)Pd(NO_(3))_(2)(M^(2)-NO_(3))(tmeda=N,N,N',N'-tetramethyl-ethylenediamine)].All complexes were characterized by NMR spectroscopy(^(1)H NMR and^(13)C NMR),mass spectrometry(CSIMS,ESI-HRMS)and elemental analysis.The single crystal X-ray diffraction analysis of[M^(1)L^(2)_2](NO_(3))_(2),[M^(1)L^(3)_2](NO_(3))_(2),[M^(1)L^(3)_2](PF_6)_2 and[M~2 L^(3)](NO_(3))_(2)further confirmed the uniquely single bowl-shape and double bowl-shape structures.The anion binding properties within the metallacalixarenes as receptors were also investigated by NMR titration experiments in DMSO. | Wutong Du Jin Tong Wei Deng Mingxue Wang Shuyan Yu | 2021 | Chinese Chemical Letters2021，32，1： | 0 |
| 20 | Plasma levels of luteinizing hormone and prolactin in relation to double brooding in Great Tit(Parus major)显示文摘The reproductive behaviors of birds are mainly controlled by the hypothalamus-pituitary-gonad axis.Many studies have shown that reproductive hormones are tightly linked to the breeding sub-stages.However,only a few studies have examined the temporal trend of hormone levels among different reproductive stages in multiple brooded species.We investigated the changes in plasma luteinizing hormone(LH)and prolactin(PRL)concentrations during different reproductive stages of the facultative double-brooded Great Tit(Parus major).We found that the concentrations of LH and PRL in females were significantly higher than those in males.Females had significantly higher LH and lower PRL concentrations in the pre-breeding period than in the first/second brooding periods,and there were no significant changes between the first and second brooding periods.The concentrations of LH and PRL in males had no significant difference between the pre-breeding period and the first brooding periods,while LH and PRL concentrations in the second brooding period were significantly higher than those in the first brooding period.We conclude that there are sex-based differences between LH and PRL at different stages of reproduction.The changes in LH and PRL in both males and females should be related to their physiological functions.Especially for males,individuals with higher levels of LH and PRL are more likely to maintain second clutches. | Xudong Li Wenyu Xu Jiangping Yu Wutong Zhang Haitao Wang | 2022 | Avian Research2022，13，1： | 0 |
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