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| 1 | Effect of L-NAME on nitric oxide and gastrointestinal motility alterations in cirrhotic rats显示文摘AIM: To invsstigare the effect of L-NAME on nitric oxide andgastriubtestubal motility alterations in cirrhotic ratsMETHODS: Rats with cirrhosis induced by carbontetrachloride were randomly divided into two groups, one( n= 13) receiving 0. 5 mg@ kg-1 per clay of NG-nitro-L-argininemethyl ester (L-NAME), a nitric oxide synthase inhibitor,for 10 days, whereas the other group ( n = 13) and control( n = 10) rats were administrated the same volume of 9 g@ L-1saline.Half gastric emptying time and 2 h residual rate weremeasured by SPECT, using 99m Tc-DTPA-labeled bariumsuifate as test meal. Gastrointestinal transition time wasrecorded simultaneously. Serum concentration of nitrcoxide (NO) was determined by the kinetic cadmiunreduction and colorimetric methods. ImmunohistochemicalSABC method was used to observe the expression anddistribution of three types of nitric oxide synthase (NOS)isoforms in the mt gastrointestinal tract. Western blot wasused to detect expression of gastrointestinal NOS isoforms.RESULTS: Half gastric emptying time and trans-gastrointestinal time were significantly prolonged( 124.0 ± 26.4min; 33.7± 8.9min;72.1 ± 15.3 min; P<0.01), (12.4±0.5h; 9.5±0.3 h; 8.2±0.8 h; P<0.01), 2h residual rate wasraised in cirrhotic rots than in controls and cirrhotic ratstreated with L-NAME(54.9± 7.6 % ,13.7 ± 3.2 %, 34.9± 10.3%, P< 0.01). Serum concentration of NO was significantlyincreased in cirrhotic rots than in the other groups (8.20 ± 2.48)μmol@L-1, (5.94± 1.07) μmol@L-1 ,and control (5.66± 1.60) tμmol@L-1, P< 0.01. NOS staining intensities which weremainly located in the gastrointestinal tissues were markedlylower in cirrhotic rats than in the controls and cirrhotic ratsafter treated with L- NAME.CONCLUSION: Gastrointestinal motility was remarkablyinhibited in cirrhotic rats, which could he alleviated by L-NAME. Nitric oxide may play an important role in theinhibition of gastrointestinal motility in cirrhotic rats. | Xin Wang Zong-You Zhang Mei Lan Ji-Yan Miao Xue-Gang Guo Yong-Quan Shi Yan-Qiu Zhao Jie Ding Kai-Cun Wu Dai-Ming Fan,Institute of Digestive disease,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China Yue-Xia Zhong,Emergency Department,Tangdu Hospital,Fourth Military Medical University,Xi’an 710038,Shaanxi Province,China Ju Lu,Class EE 87,Department of Electronic Engineering,Tsinghua University,Beijing 100084,China Bo-Rong Pan,Oncology Center,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China | 2002 | World Journal of Gastroenterology2002，8，2： | 19 |
| 2 | Nat Chem Biol:利用CRISPR-Cas9激活细菌中沉默的基因簇,有望发现新的药物显示文摘为了抵抗疾病,很多医药库中的武器是从细菌当中获得的。如今,利用CRISPR-Cas9基因编辑技术。研究人员揭示出沉默基因中隐藏着的更多潜在的宝藏。作为一类常见的细菌。链霉菌被用来产生很多作为抗生素、抗癌试剂和其他药物的化合物。在一项新的研究中。来自美国伊利诺伊大学和新加坡科技研究局的研究人员利用CRISPR-Cas9技术激活链霉菌中不表达的或者说沉默的基因簇。 | Mingzi M Zhang, Wan Lin Yeo, Ee Lui Ang, Huimin Zhao Fong Tian Wong, Elena Heng Yajie Wang, Shangwen Luo, Ryan E Cobb, Behnam Enghiad, Huimin Zhao Yee Hwee Lim | 2017 | 现代生物医学进展2017，17，17： | 12 |
| 3 | A major pathway for carbon and nitrogen losses-Gas emissions during storage of solid pig manure in China显示文摘This study investigated the carbon(C) and nitrogen(N) gas emissions(N_2O,NH_3,CO_2 and CH_4) from solid pig manure management in China.Gas emissions were quantified from static piles over 60 days during summer in China's Yangtze River Basin,using Drager-Tube and static chamber-gas chromatography techniques.High emissions of NH_3 and N_2 O were observed at the early stage of storage,but high emission of CH_4 occured later during storage.Overall,62% of the total C in the original pile was lost; CO_2 and CH_4 emissions accounted for 57 and 0.2% of C lost respectively.Over the same time,41% of the total N in the original pile was lost; NH_3 and N_2 O emissions accounted for 15 and 0.3% of N lost respectively.The volatilization of NH_3 during storage in summer was 4.56 g NH_3 per kg dry weight.The total greenhouse gas(GHG) emissions during storage accounted for 67.93 g CO_2 equivalent per kg dry weight; N_2 O and CH_4 contributed to 46 and 55% of total GHG emissions respectively.Given China's major role in pig production,further attention should given to pig manure management to mitigate its contribution to atmospheric pollution. | SHAN Nan LI Hu LI Jian-zheng Ee Ling Ng MA Yan WANG Li-gang CHEN Qing | 2019 | Journal of Integrative Agriculture2019，18，1： | 3 |
| 4 | Protein and RNA expression of acid-sensing ion channels 2 and 3 in myocardial ischemia rats induced by isoproterenol显示文摘OBJECTIVE:To investigate the impact of electro-acupuncture at the Neiguan(PC 6) acupoint on protein and RNA expression of acid-sensing ion channel 2(ASIC2) and ASIC3 in myocardial ischemia rats.METHODS:Fifty male Sprague-Dawley rats were used,weighing(230 ± 50) g.The rats were randomized into a normal group A,model group B,Neiguan(PC 6) group C,Lieque(LU 7) group D,and A-shi points group E.There were 10 rats in each group.Rats were continuously administered 85 mg/kg intravenous isoproterenol daily to establish the model.Successfully modeled rats in groups C,D,and E were given electro-acupuncture treatment.Each group of rats was sacrificed with chloral hydrate(1 mL/100 g) intraperitoneal injection.The left ventricular myocardium was extracted and placed at- 70 ℃ until use.Western blot analysis and real-time PCR were performed to assay protein and RNA expressions of ASIC2 and ASIC3,respectively.Fold changes in RNA expression were quantified with the 2~^(-△△Ct) method.Blood samples were drawn from the aorta abdominalis and tested for creatine kinase-MB(CK-MB) and lactate dehydrogenase(LDH) levels using enzyme-linked immunosorbent assay.RESULTS:Myocardial ischemia rats given electro-acupuncture at the Neiguan(PC 6) acupoint had significantly lower protein and RNA expression of ASIC2 and ASIC3,and CK-MB and LDH levels,compared with model rats(P < 0.01).CONCLUSION:Electro-acupuncture at the Neiguan(PC 6) acupoint can not only decrease the protein and RNA expression of ASIC2 and ASIC3,but also inhibit the opening of ASICs and reduce the cardiomyocyte damage in myocardial ischemia rats. | Wang Shudong Zhang Lide Dong Baoqiang Zhang Wenshun Ho Chin Ee Guo Na Chen Yiguo | 2015 | Journal of Traditional Chinese Medicine2015，35，2： | 2 |
| 5 | The retinoblastoma tumor suppressor modifies the therapeutic response of breast Canc- er 显示文摘 | Bosco EE Wang Y Xu H | 2007 | J Clin Invest2007，117，1： | 1 |
| 6 | The critical period for susceptibility of human stereopsis显示文摘 | Fawcett SF Wang YZ Birch EE | | 0，，06： | 1 |
| 7 | Transforming growth factor- beta mRNA and protain in hypertrophic scar tissues and fibroblasts antagonism by IFN alpha and IFN- gamma in vitro and in vivo显示文摘 | Tredget EE Wang R Shen Q | 2000 | J Interferon Cytokine Res2000，20，2： | 1 |
| 8 | TGF-β mRNA and protein in hypertrophic scartissue and tqbroblasts 显示文摘 | Tredget EE Wang R Shen Q | 2000 | J Interferon Cytokine Res2000，20，2： | 1 |
| 9 | Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases显示文摘 | Perez EE Wang JB Miller JC | | 0，，： | 1 |
| 10 | Ureaplasma urealyticum infections in the perinatal period显示文摘 | Wang EE Matlow AG Ohlsson A Nelson SC | 1997 | Clin Perinatol1997，24，： | 1 |
| 11 | Dynamic signals for hair follicle development and regeneration 显示文摘 | Wang X Tredget EE Wu Y | 2012 | Stem Ceils Dev2012，21，1： | 1 |
| 12 | Underlying causes of recurrent Pneumonia in children显示文摘 | Owayed AF CamPbell DM Wang EE | 2000 | Arch Pediatr Ado- lesc Med2000，154，2： | 1 |
| 13 | Reliability of the chest radiograph in the diagnosis of lower respiratory infections in young children显示文摘 | Davies HD Wang EE Manson D | 1996 | Pediatr Infect Dis J1996，15，7： | 1 |
| 14 | Underlying causes of recurrent pneumonia in children显示文摘 | Owayed AF Campbell DM Wang EE | 2000 | Arch Pediatr Adolesc Med2000，154，2： | 1 |
| 15 | Antibiotic prcscribing for upper respiratory tract infection:the importance of diagnostic uncertainty显示文摘 | Arnold SR To T Mclsaac WJ Wang EE | 2005 | J Pediatr2005，146，2： | 1 |
| 16 | Acute otitis media severity of symptom score in a tympanocenesis study显示文摘 | Shaikh N Wang EE Arguedas A | 2011 | Pe- diatr Infect Dis J2011，30，： | 1 |
| 17 | Dimerization, DNA binding, and transactivation properties of hypoxia inducible factor 1 显示文摘 | Jiang BH Ru EE Wang GL | 1996 | J Biol Chem1996，271，17： | 1 |
| 18 | Oscillatory Lande factor of two- dimensional electrons under high magnetic fields 显示文摘 | Mendez EE Nocera J Wang Vii | 1993 | Phys Rev B Condens Matter1993，47，13： | 1 |
| 19 | Parameterization and parametric design of mannequins显示文摘 | CHARLIE EE WANG CL | 2005 | Computer-Aided Design2005，，1： | 1 |
| 20 | Underlying causes of recur- rent pneumonia in children显示文摘 | Owayed AF Campbell DM Wang EE | 2000 | Arch Pediatr Adolesc Med2000，154，2： | 1 |
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