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| 1 | An equation of entransy transfer and its application显示文摘Entransy is a physical quantity describing heat transfer ability, and heat transfer is accompanied by entransy transfer. Thermal energy is conserved in its transfer process, while entransy is dissipated because of the irreversibility of its transfer process. As a result, entransy transfer must have its rules which are different from those of thermal energy transfer. Based on the definition of entransy, an entransy transfer equation is derived, which describes the entransy transfer processes of a multi-component viscous fluid subject to heat transfer by conduction and convection, mass diffusion and chemical reactions. The expressions of entransy flux and entransy dissipation are obtained simultaneously, and their physical mechanism is clarified. And further, the theory and method of optimizing heat transfer applying the entransy transfer equation to the steady-state convection heat transfer process are expounded. The minimum thermal resistance principle and the entransy dissipation extremum principle are obtained by applying the steady-state entransy transfer equation to the steady-state convection heat transfer process. The cases of the single-component steady-state convection heat transfer and the steady-state heat conduction show the application of the theory and method. | WANG SongPing CHEN QingLin ZHANG BingJian | 2009 | Chinese Science Bulletin2009，54，19： | 23 |
| 2 | A general theoretical principle for single-phase convection heat transfer enhancement显示文摘The main methods of single-phase convection heat transfer enhancement are analyzed in this paper, and the unity of contradiction between heat transfer enhancement and energy consumption(or exergy destruction)is expounded.The thermodynamic relationship between heat(or exergy)transfer efficiency and energy consumption(or exergy destruction)as well as driving forces is established,and a general theoretical principle for single-phase convection heat transfer enhancement is further obtained. The principle shows that temperature gradient field distribution and velocity field distribution constrain each other,and that the optimum heat transfer efficiency can be obtained when they are synergetic.If the level of the synergy of temperature gradient field distribution with velocity field distribution is determined,the relative uniform temperature gradient is required,and vice versa.The principle also shows the relationship of relative temperature gradient with specific heat and coefficient of heat conductivity.The deduced results can be used as a theoretical guidance for single-phase convection heat transfer enhancement and optimum design of heat exchangers. | WANG SongPing CHEN QingLin ZHANG BingJian HUA Ben | 2009 | Science China(Technological Sciences)2009，52，12： | 12 |
| 3 | Positional assembly of multi-enzyme cascade reaction in polyelectrolyte doped microcapsule through electrospray and layer-by-layer assembly显示文摘Polyelectrolyte-doped microcapsules(PDM)was fabricated by coaxial electrospray of a mixture of glycerol and water containing 10 mg/mL cationic polyelectrolyte poly(allylamine hydrochloride)(PAH)fed as the core phase solution,and a N,N-dimethylacetylamide solution of 10 wt%polyurethane fed as the shell phase solution.Multienzyme system involving Candida Antarctica lipase B(CALB),glucose oxidase(GOD),and horseradish peroxidase(HRP)for cascade reaction was assembled in the PDM at three different places,namely,surface,shell,and lumen.Placing of enzyme inside aqueous lumen of the PDM was realized by in situ encapsulation through adding the enzyme in the core-phase solution for coaxial electrospray.By ion-pairing of enzyme with cationic surfactant CTAB,an organic soluble enzyme-CTAB complex was prepared,so that in situ embedding of enzyme in the shell of the PDM was realized by adding it into the shell phase solution.Surface attachment of enzymes was achieved by layer-by-layer(LbL)technology,which is based on the ion-exchange interactions between oppositely charged enzymes and PAH that was doped in PDM.The enzyme-decorated microcapsule was then studied as a microbioreactor,in which 1-Oxododecyla-α-glucopyranoside was converted by CALB to glucose,which was oxidised by GOD to gluconolactone in a second step.The hydrogen peroxide produced was then used by HRP to oxidize ABTS to form coloured radical cation ABTS•+for activity analysis.The successful fabrication of the PDM and precise localization of enzymes in the PDM by different strategies were fully characterized.By varying the immobilization strategy,totally six PDM bioreactors with three enzymes precisely positional assembled in different strategies were constructed and their activities for the cascade reaction were investigated and compared.The PDM micro-bioreactor prepared by novel electrospray technologies provide a smart platform for positional assembly of multi-enzyme cascade reaction in a precise and well-controlled manner. | Shiyi Che Jie Wang Xiaoyuan Ji Zhiguo Su Shaomin Wang Songping Zhang | 2020 | Synthetic and Systems Biotechnology2020，5，3： | 2 |
| 4 | Evaluation of strategy for analyzing mouse liver plasma membrane proteome显示文摘Plasma membrane (PM) proteome is one of the major subproteomes present in the cell,and is very important in liver function. In the present work, C57 mouse liver PM was purified by density-gradient centrifugation. The purified PM was verified by electron microscope analysis and Western blotting. The results showed that the PM was enriched by more than 20-fold and the contamination of mitochondria was reduced by 2-fold compared with the homogenization fraction. Proteins were separated by 2DE and 1DE, trypsin-digested and submitted to ESI-Q-TOF and MALDI-TOF-TOF mass spectrometry or directly digested in solution and analyzed by LC-ESI ion trap mass spectrometry. In all, 547 non-redundant mouse liver PM proteins were identified, of which 34% contributed to plasma membrane or plasma membrane-related proteins. This study optimized and evaluated the HLPP plasma membrane proteome analysis method and made a systematic analysis on PM proteome. | CHEN Ping ZHANG LiJun LI XuanWen WANG XiE CAO Rui LIU Zhen XIONG JiXian PENG Xia WEI YingJuan YING XingFeng WANG XianChun LIANG SongPing | 2007 | Science China(Life Sciences)2007，50，6： | 1 |
| 5 | Sample preparation for the analysis of membrane proteomes by mass spectrometry显示文摘The low abundance and highly hydrophobic nature of most membrane proteins make their analysis more difficult than that for common soluble proteins.Successful membrane protein identification is largely dependent on the sample preparation including the enrichment and dissolution of the membrane proteins.A series of conventional and newly developed methods has been applied to the enrichment of low-abundance membrane proteins at membrane and/or protein levels and to the dissolution of hydrophobic membrane proteins.However,all the existing methods have inherent advantages and limitations.Up to now,there has been no unique method that can universally be employed to solve all the problems and more efforts are needed in improving sample preparation for the analysis of membrane proteomes. | Xianchun Wang Songping Liang | 2012 | Protein & Cell2012，3，9： | 1 |
| 6 | Environmental and canopy conditions regulate the forest floor evapotranspiration of larch plantations显示文摘Background:Integrated forest-water management focusing on forest-water coordination is an important way to alleviate water use conflicts among forests and other sectors in vast dryland regions.Forest floor evapotranspiration(FE),which is an important component of forest evapotranspiration,accounts for a large proportion of the water consumed in arid forests.Elucidating how environmental and canopy conditions impact FE has important significance for guiding integrated forest-water management in a changing environment.Methods:The microlysimeter(ML)-measured evapotranspiration(FE_(ML)),reference evapotranspiration(ET_(o)),volumetric soil moisture(VSM),and canopy leaf area index(LAI)were monitored in a Larix principis-rupprechtii plantation located in the semi-humid Liupan Mountains of Northwest China in 2019(June–September)and 2021(May–September).The response functions of the FE coefficient(the ratio of daily FEML to ET_(o))to the individual factors of VSM and LAI were determined using upper boundary lines of scatter diagrams of measured data.The framework of the daily FE(FE_(ML))model was established by multiplying the response functions to individual factors and then calibrated and validated using measured data to assess the FE response to environmental and canopy conditions.Results:(1)The FE coefficient increased first rapidly and then slowly with rising VSM but decreased slowly with rising LAI.(2)The simple daily FE(FE_(ML))model developed by coupling the impacts of ET_(o),LAI,and VSM in this study performed well for predicting FE.(3)The impacts of ET_(o),LAI,and VSM were quantified using the FE(FE_(ML))model,e.g.,at a given VSM,the impact of ETo on FE increased obviously with decreasing LAI;at a given ET_(o),the impact of LAI on FE increased with rising VSM.(4)In the two study years,when directly using the microlysimeter measurement,the real FE on the forest floor was overestimated when the VSM in microlysimeters was above 0.215 but underestimated below 0.215 due to the difference in VSM from the forest floor.Thus,the VSM on the forest floor should be input into the FE model for estimating the real FE on the forest floor.Conclusions:The daily FE of larch plantation is controlled by three main factors of environmental(ET_(o) and VSM)and canopy conditions(LAI).The variation in daily FE on the forest floor can be well estimated using the simple FE model coupling the effects of the three main factors and by inputting the VSM on the forest floor into the model to avoid the errors when directly using the microlysimeter measurement with different VSMs from the forest floor.The developed FE model and suggested prediction approach are helpful to estimate the FE response to changing conditions,and to guide forest management practices when saving water by thinning is required. | Zebin Liu Yanhui Wang Pengtao Yu Lihong Xu Songping Yu | 2022 | Forest Ecosystems2022，9，5： | 1 |
| 7 | Heat transfer enhancement and optimum design of the new approach显示文摘 | Wang Songping Luo Weiping Chen Qingping | 2004 | Journal of North China Electric Power University2004，31，6： | 1 |
| 8 | Mechanisms of peroxynitrite-induced [Ca^(2+)]_i increase in single neuronal cell显示文摘The mechanism of peroxynitrite (ONOO -)induced [Ca 2+ ] i increase in single MN9D cell (Dopaminergic neuroblastoma cell line) was studied by using Fura2 microfluorometric technique. The results show that ONOO - caused a rapid increase of [Ca 2+ ] i when ONOO - was puffed to the cell. Removing Ca 2+ from the bath or using calcium channel antagonist (CdCl 2, Nifedipine) greatly inhibited the [Ca 2+ ] i increase induced by ONOO -, suggesting that the opening of LCa 2+ channel makes a great contribution to the [Ca 2+ ]\-i increase. The effect of sulfhydryl reductive agent (DTT) on ONOO -induced [Ca 2+ ]\-i increase suggests that ONOO -activating LCa 2+ channel is partly related to its oxidative speciality. | YI Yong 1, CAI Dong 1, GAO Zhonghong 1, YANG Xiangliang 1, QU Anlian 1, XU Huibi 1* , WANG Xiaomin 2 and HAN Songping 3 1. Institute of Biophysics and Biochemistry, Huazhong University of Science and Technology, Wuhan 430074, China 2. Neuros | 1998 | Chinese Science Bulletin1998，43，20： | 1 |
| 9 | De nove sequencing of tryptic peptides sulfonated by 4-sulfophenyl isothiocyanate for unambiguous protein identification using post-source decay matrix-assisted laser desorption/ionization mass spectrometry显示文摘 | Chen Ping Nie Song Wang Xiangchun Liang Songping | 2004 | Rapid Commun Mass Spectrom2004，18，： | 1 |
| 10 | Additive, dominance genetic effects for growth-related traits in common carp, Cyprinus carpio L 显示文摘 | Wang Chenghui Li Sifa Xiang Songping | 2006 | Aquaculture Research2006，37，4： | 1 |
| 11 | Parental genetic effects evaluation for growth-related traits of red common carp in China 显示文摘 | Wang Chenghui Wang Jun Xiang Songping | 2009 | Fish Sci2009，75，： | 1 |
| 12 | pIL-12 delivered by polymer based nanovector for anti-tumor genetherapy显示文摘Finding more effective and safe non-viral vectors to transfer genes into cancer cells has become the key of immune gene therapy for cancer.Herein a triblock compound MPEG_(2000)-PDLLA_(4000)-MPEG_(2000) modified by cationic liposome DOTAP was used as a non-viral vector DOTAP/MPEG_(2000)-PDLLA_(4000)-MPEG_(2000)(DMPM)to effectively transfer interleukin(IL)-12 plasmid(pIL-12)into tumor tissue.IL-12 produced by transfected tumor cells successfully inducing lymphocyte proliferation and promoting interferon-γ(IFN-γ)secretion,which resulted in tumor cells death.The ability of DMPM to transfer pIL-12 and the immune effect induced by IL-12 in cells had been explored.The anti-tumor effect,mechanism and safety of pIL-12/DMPM in mice cancer model were investigated in this study.Our results showed that the pIL-12 transferred by DMPM was highly expressed both in CT26 cells and B16-F10 cells.IL-12 expressed in the culture supernatant of transfected tumor cells stimulated lymphocyte proliferation and promoted IFN-γsecretion.The experimental result confirmed that pIL-12/DMPM therapy significantly reduced tumor growth in mice model.We designed the nanocomposite DMPM to deliver pIL-12 for cancer treatment and explored its therapeutic efficacy and the underlying anti-tumor mechanism.Our study suggested pIL-12 loaded by DMPM complex would be an effective strategy for cancer treatment. | Lianbin Wen Xin Zan Qidi Pang Yuzhu Hu Songping Zheng Mengni Ran Xiang Gao Xiang Wang Bilan Wang | 2023 | Chinese Chemical Letters2023，34，11： | 0 |
| 13 | Impacts of environmental and canopy conditions on the nighttime sap flow of larch plantations in the Liupan Mountains,China显示文摘Nighttime sap flow(Q_(n))is an important physiological activity under which trees manage drought stress.An in-depth understanding of the characteristics of Q_(n)and its response to environmental and canopy conditions are of significance for arid area forest and water management.This study measured daily sap flow(Q_(s))of a Larix principis-rupprechtii plantation in the Liupan Mountains,northwest China during the 2017-2019 growing seasons,and separated Q_(s)into daytime sap flow(Qd)and Q_(n).Meteorological conditions(reference evapotranspiration,ETref),canopy structure(leaf area index,LAI),and soil moisture(relative soil water content,RSWC)were considered as the main biophysical factors affecting Q_(n).The structural equation model and upper boundary line method determined the effects of compound and single factors on Q_(n)The daily mean Q_(n)values during the growing seasons in 2017,2018,and 2019 were 0.024,0.026,and 0.030 mm d-1,accounting for 6.2,11.2,and 10.1%of Q_(s),respectively.Q_(n)at different canopy development phases(leaf expanding,LG;leaf expanded,LD;and defoliation,DF)over three years was LD>LG>DF.Q_(n)increased with increasing ETref,whereas the ratio of Q_(n)to Q_(s)decreased.Q_(n)did not show regular variation in the three-year growing seasons under different soil moisture conditions.ETrefand LAI mainly controlled Q_(n)by affecting Qd,whereas RSWC had no significant effect on Q_(n).Q_(n)had a positive and linear relationship with LAI and a quadratic relationship with ETref.Both explained 40%of variation in Q_(n)Meteorological and canopy conditions are important factors affecting Q_(n)on the semi-humid study site.The application of the Q_(n)model coupled with the impact of ETrefand LAI furthers understanding of the impacts of climate and forest structure change on Q_(n). | Songping Yu Jianbin Guo Zebin Liu Yanhui Wang Lihong Xu Pengtao Yu Liang He | 2023 | Journal of Forestry Research2023，34，6： | 0 |
| 14 | Leaf Morphology Genes SRL1 and RENL1 Co-Regulate Cellulose Synthesis and Affect Rice Drought Tolerance显示文摘The morphological development of rice(Oryza sativa L.)leaves is closely related to plant architecture,physiological activities,and resistance.However,it is unclear whether there is a co-regulatory relationship between the morphological development of leaves and adaptation to drought environment.In this study,a drought-sensitive,roll-enhanced,and narrow-leaf mutant(renl1)was induced from a semi-rolled leaf mutant(srl1)by ethyl methane sulfonate(EMS),which was obtained from Nipponbare(NPB)through EMS.Map-based cloning and functional validation showed that RENL1 encodes a cellulose synthase,allelic to NRL1/OsCLSD4.The RENL1 mutation resulted in reduced vascular bundles,vesicular cells,cellulose,and hemicellulose contents in cell walls,diminishing the water-holding capacity of leaves.In addition,the root system of the renl1 mutant was poorly developed and its ability to scavenge reactive oxygen species(ROS)was decreased,leading to an increase in ROS after drought stress.Meanwhile,genetic results showed that RENL1 and SRL1 synergistically regulated cell wall components.Our results revealed a theoretical basis for further elucidating the molecular regulation mechanism of cellulose on rice drought tolerance,and provided a new genetic resource for enhancing the synergistic regulation network of plant type and stress resistance,thereby realizing simultaneous improvement of multiple traits in rice. | LIU Dan ZHAO Huibo WANG Zi’an XU Jing LIU Yiting WANG Jiajia CHEN Minmin LIU Xiong ZHANG Zhihai CEN Jiangsu ZHU Li HU Jiang REN Deyong GAO Zhenyu DONG Guojun ZHANG Qiang SHEN Lan LI Qing QIAN Qian HU Songping ZHANG Guangheng | 2024 | Rice science2024，31，1： | 0 |
| 15 | Thermocapillary flow-induced core release from double-emulsion droplets in microchannels显示文摘The use of double emulsions(DEs),which represent colloidal structures composed of droplets nested within droplets,can provide for unparallel droplet manipulation in droplet-based microfluidic technology due to their unique core–shell structures.The controlled release of cores in DEs is of particular interest.However,this process remains poorly explored.In this work,the thermocapillary flow induced by a temperature gradient is used as a driving force to control the core release and the impacts of different linear temperature gradients,core diameters,shell diameter,and core/shell diameter ratios on the thermocapillary flow and core release characteristics of DE droplets consisting of a water-in-n-hexadecane-in-water system within a cylindrical microchannel are investigated.Most of the core and shell diameter conditions considered result in a double-core release process,where the inner droplet volume is partially ejected before the remaining core is rewrapped by the outer droplet,and the remaining inner droplet volume is ejected later during a second core release event.However,relatively small core diameters of 50 and 75μm produce conditions where the full inner droplet volume is ejected during a singlecore release process.In addition,we provide empirical relationships for accurately determining the time at which core release initially occurs under given DE parameters as well as for precisely determining whether the applied conditions will lead to single-or double-core release processes.Therefore,the results of this study provide insights enabling the development of accurate inner droplet release technologies under thermocapillary migration. | Jingyi Qin Zhibin Wang Ying Chen Songping Mo Jian Liu | 2023 | Droplet2023，2，3： | 0 |
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