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| 1 | Isolation and diversity analysis of heterotrophic bacteria associated with sea anemones显示文摘A study was undertaken to investigate the heterotrophic bacterial flora associated with the sea anemones.Samples of the sea anemones Anthopleura midori were collected from the coast of Weihai and bacteria were isolated from these samples.Additionally,high numbers of viable bacteria were obtained from the celom wall and surface of anemone,the community of cultivable bacteria was very diverse.As a result of this isolation,60 strains were obtained,56 of them were selected for identification and characterization by 16S rRNA gene sequence analysis and limited phenotypic testing.Among these isolates,16 strains were phylogenetically related to members of the genus P seudoalteromonas and neighboring taxa.Other isolates included members of the genera Colwellia,Vibrio,Acinetobacter,Pseudomonas,Endozoicomonas,Roseovarius,Paracoccus,Loktanella,Leisingera,Sulfitobacter,Bacillus,Staphylococcus,Plantibacter,Microbacterium,Micrococcus,Joostella,Psychroserpens,Cellulophaga,Krokinobacter,Polaribacter and P sychrobacter.Seven potential novel species were found.Among 60 strains,17 of them can produce proteolytic exoenzyme,20 can produce lipolytic exoenzyme.Strain NQ8 has strong antagonistic effects on some Vibrio strains.This study demonstrates that the culturable fraction of bacteria from the sea anemones Anthopleura midori is diverse and appears to possess much potential as a source for the discovery of novel bioactive materials. | DU Zongjun ZHANG Wanyi XIA Hongjie LU Guoqiang CHEN Guanjun | 2010 | Acta Oceanologica Sinica2010，29，2： | 3 |
| 2 | Unexpected fast radical production emerges in cool seasons:implications for ozone pollution control显示文摘Ozone is a crucial air pollutant that damages human health and vegetation.As it is related to the photo-oxidation of the nitrogen oxides and volatile organic compounds,the summertime reduction of these precursors is the primary focus of current ozone mitigation strategies.During ozone pollution episodes in eastern China,an observed accumulation of daily total oxidants(O_(x)=NO_(2)+O_(3))in cool seasons(spring and autumn:60 ppb and winter 40 ppb)is comparable to that in summer(60 ppb),indicating fast photochemical production of secondary pollutants including ozone over the year.Unrecognized fast radical primary productions are found to counteract the increased termination of hydroxyl radical and unfavorable meteorological conditions to maintain the rapid total oxidant formations in cool seasons.Elucidating and regulating the primary radical sources may be critical for the secondary air pollution control in cool seasons. | Hongli Wang Yanhui Liu Xiaorui Chen Yaqin Gao Wanyi Qiu Shengao Jing Qian Wang Shengrong Lou Peter MEdwards Cheng Huang Keding Lu | 2022 | National Science Open2022，1，2： | 2 |
| 3 | Recent Progress on 2D Transition Metal Compounds-based Electrocatalysts for Efficient Nitrogen Reduction显示文摘Ammonia is a commodity chemical with high added value.Electrochemical reduction of nitrogen has great promise for the sustainable synthesis of ammonia in recent vears.Because of its rich resources and unique electronic structure and characteristics,2D transition metal compounds have been used as electrocatalysts for electro-chemical reduction of nitrogen for clean and sustainable production of ammonia,This review outlines the latest development in the use of 2D transition metal compounds as high-efficiency electrocatalysts for nitrogen reduction.reaction(NRR).First,we introduce the N,reduction mechanism,and briefly summarize the performance indicators ofthe catalyst.Then,we focused on the functionalization of unique 2D materials to design high-performance 2D electrocatalysts in respect of simulation calculation and experimental development.Finally,the current challenges and future opportunities for NRR electrocatalvsts are introduced. | DONG Wcnfei CHEN Xiaoyu PENG Juan LIU Wanyi JIN Xiaoyong NI Gang LIU Zheng | 2020 | Chemical Research in Chinese Universities2020，36，4： | 2 |
| 4 | Direct control of store-operated calcium channels by ultrafast laser显示文摘Ca^(2+) channels are essential to cell birth,life,and death.They can be externally activated by optogenetic tools,but this requires robust introduction of exogenous optogenetic genes for expression of photosensitive proteins in biological systems.Here we present femtoSOC,a method for direct control of Ca^(2+)channels solely by ultrafast laser without the need for optogenetic tools or any other exogenous reagents.Specifically,by focusing and scanning wavelength-tuned low-power femtosecond laser pulses on the plasma membrane for multiphoton excitation,we directly induced Ca^(2+) influx in cultured cells.Mechanistic study reveals that photoexcited flavins covalently bind cysteine residues in Orai1 via thioether bonds,which facilitates Orai1 polymerization to form store-operated calcium channels(SOCs)independently of STIM1,a protein generally participating in SOC formation,enabling all-optical activation of Ca^(2+) influx and downstream signaling pathways.Moreover,we used femtoSOC to demonstrate direct neural activation both in brain slices in vitro and in intact brains of living mice in vivo in a spatiotemporal-specific manner,indicating potential utility of femtoSOC. | Pan Cheng Xiaoying Tian Wanyi Tang Juan Cheng Jin Bao Haipeng Wang Sisi Zheng Youjun Wang Xunbin Wei Tunan Chen Hua Feng Tian Xue Keisuke Goda Hao He | 2021 | Cell Research2021，31，7： | 2 |
| 5 | Retention of Vibrio parahaemolyticus in oyster tissues after chlorine dioxide treatment显示文摘 | Dapeng Wang Dandan Zhang Wanyi Chen Shuijing Yu Xianming Shi | 2009 | International Journal of Food Microbiology2009，，1： | 2 |
| 6 | Development of a real time PCR assay for rapid detection of Vibrio parahaemolyticus from seafood显示文摘A real time PCR assay for the detection of Vibrio para-haemolyticus in seafood samples was developed using a novel specific target and a competitive internal ampli-fication control(IAC).The specificity of this assay was evaluated using 390 bacterial strains including V.parahaemolyticus,and other strains belonging to Vibrio and non-Vibrio species.The real time PCR assay un-ambiguously distinguished V.parahaemolyticus with a detection sensitivity of 4.8 fg per PCR with purified genomic DNA or 1 CFU per reaction by counting V.parahaemolyticus colonies.The assays of avoiding interference demonstrated that,even in the presence of 2.1μg genomic DNA or 107 CFU background bacteria,V.parahaemolyticus could still be accurately detected.In addition,the IAC was used to indicate false-negative results,and lower than 94 copies of IAC per reaction had no influence on the detection limit.Ninety-six sea-food samples were tested,of which 58(60.4%)were positive,including 3 false negative results.Conse-quently,the real time PCR assay is effective for the rapid detection of V.parahaemotyticus contaminants in seafood. | Bin Liu Xiaohua He Wanyi Chen Shuijing Yu Chunlei Shi Xiujuan Zhou Jing Chen Dapeng Wang Xianming Shi | 2012 | Protein & Cell2012，3，3： | 2 |
| 7 | Vertical Profiles of Volatile Organic Compounds in Suburban Shanghai显示文摘As Volatile Organic Compounds(VOCs)are one of the precursors of ozone,their distribution and variable concentrations are highly related to local ozone pollution control.In this study,we obtained vertical profiles of VOCs in Shanghai’s Jinshan district on 8 September and 9 September in 2016 to investigate their distribution and impact on local atmospheric oxidation in the near surface layer.Vertical samples were collected from heights between 50 m and 400 m by summa canisters using an unmanned aerial vehicle(UAV).Concentrations of VOCs(VOCs refers to the 52 species measured in this study)varied minimally below 200 m,and decreased by 21.2%from 100 m to 400 m.The concentrations of VOCs above 200 m decreased significantly in comparison to those below 200 m.The proportions of alkanes and aromatics increased from 55.2%and 30.5%to 57.3%and 33.0%,respectively.Additionally,the proportion of alkenes decreased from 13.2%to 8.4%.Toluene and m/p-xylene were the key species in the formation of SOA and ozone.Principal component analysis(PCA)revealed that the VOCs measured in this study mainly originated from industrial emissions. | Yuhan LIU Hongli WANG Shengao JING Ming ZHOU Shenrong LOU Kun QU Wanyi QIU Qian WANG Shule LI Yaqin GAO Yusi LIU Xiaobing LI Zhong-Ren PENG Junhui CHEN Keding LU | 2021 | Advances in Atmospheric Sciences2021，38，7： | 1 |
| 8 | A New Optical Fiber Loop System Used in Measurement of Dispersion Compensation Ability显示文摘ANewOpticalFiberLoopSystemUsedinMeasurementofDispersionCompensationAbilityManuscriptreceivedDec.24,1996.SupportedbyKeyProject... | Sun Jian, Gu Wanyi, Chen Xue, Li Guorui and Xu Daxiong (Telecommunication Engineering Department, Beijing University of Posts and Telecommunications, Beijing 100876, P.R.China) | 1997 | The Journal of China Universities of Posts and Telecommunications1997，4，2： | 1 |
| 9 | Molecular typing of Vibrio parahaemolyticus isolates from the middle-east coastline of China显示文摘 | CHEN Wanyi XIE Yanping XU Jingye | 2012 | International Journal of Food Microbiology2012，153，3： | 1 |
| 10 | On the H_∞ Control for Linear Time-Delay Systems显示文摘In this paper, we study the H∞ control of time-delay linear systems either with some norm-bounded uncertainties or not with (for systems not in the scope of the nominal systems of the former ones). As linear time-delay systems are infinite dimensional in natural, some new sufficient conditions in Riccati equation form are offered, which extends current related results. We also point out a mistake appeared in a recently published paper. | Chen Wanyi (College of Mathematics, Nankai University, Tianjin 300071, P. R. China ) | 1998 | Journal of Systems Engineering and Electronics1998，9，3： | 1 |
| 11 | Implementing the free HPV vaccination for adolescent girls aged below 14 in Shenzhen,Guangdong Province of China:experience,challenges,and lessons显示文摘Cervical cancer is a major public health concern in China,accounting for almost one-fifth of the global incidence and mortality.The recently prequalified domestic bivalent human papillomavirus(HPV)vaccine offers a practical and feasible preventive measure.In response to the global call for action,the National Health Commission issued an Action Plan to eliminate cervical cancer by 2030,with promotion of the HPV vaccination for school-aged girls as a critical step.Despite this,implementation of the vaccination has been patchy,with very low coverage among eligible girls.To address this,from December 2021 to December 2022,a demonstration project was launched in Shenzhen,Guangdong Province,to promote the inclusion of HPV vaccine in local immunisation programme and to address existing barriers to implementation.Using multiple sources of data,this article presents a case study of the demonstration project,analysing its impact on rolling out HPV vaccination among eligible girls and identifying any challenges encountered during implementation.The demonstration project has shown promising results in increasing the HPV vaccination rate,promoting public awareness and acceptance of the domestic HPV vaccine,and establishing a model for quickly scaling up the vaccination at the municipal level.The success of the project can be attributed to several factors,including strong governmental commitment,sufficient funding,multi-sectoral collaboration,ensured vaccine accessibility and affordability,improved vaccination services,and effective health education and communication strategies.Lessons learned from Shenzhen can provide valuable insights for future advocacy and implementation of the vaccination in other areas of China,but challenges must be addressed to achieve universal coverage.These include addressing vaccine hesitancy,expanding the programme to cover a broader age range,and ensuring consistent quality of vaccination services in primary care facilities.Overcoming these challenges will require innovative strategies,public-private partnerships,and sustained funding and resources.Future research should focus on evaluating the long-term effectiveness of the vaccination programme and identifying contextual factors that may impact its implementation in different settings.Overall,the effective control of cervical cancer in China will rely on the“political will”to ensure the incorporation of preventive interventions into policies and universal programme coverage. | Dadong Wu Peiyi Liu Danhong Song He Wang Siqi Chen Wanyi Tang Xuelian Zhao Fanghui Zhao Yueyun Wang | 2023 | Infectious Diseases of Poverty2023，12，5： | 0 |
| 12 | Dicalcium silicate-induced mitochondrial dysfunction and autophagy-mediated macrophagic inflammation promotes osteogenic differentiation of BMSCs显示文摘Dicalcium silicate(Ca_(2)SiO_(4),C_(2)S)has osteogenic potential but induces macrophagic inflammation.Mitochondrial function plays a vital role in macrophage polarization and macrophagic inflammation.The mitochondrial function of C_(2)S-treated macrophages is still unclear.This study hypothesized:(i)the C_(2)S modulates mitochondrial function and autophagy in macrophages to regulate macro-phagic inflammation,and(ii)C_(2)S-induced macrophagic inflammation regulates osteogenesis.We used RAW264.7 cells as a model of macrophage.The C_(2)S(75–150μg/ml)extract was used to analyze the macrophagic mitochondrial function and macrophagemediated effect on osteogenic differentiation of mouse bone marrow-derived mesenchymal stem cells(BMSCs).The results showed that C_(2)S extract(150μg/ml)induced TNF-α,IL-1βand IL-6 production in macrophages.C_(2)S extract(150μg/ml)enhanced reactive oxygen species level and intracellular calcium level but reduced mitochondrial membrane potential and ATP production.TEM images showed reduced mitochondrial abundance and altered the mitochondrial morphology in C_(2)S(150μg/ml)-treated macrophages.Protein level expression of PINK1,Parkin,Beclin1 and LC3 was upregulated but TOMM20 was downregulated.mRNA sequencing and KEGG analysis showed that C_(2)S-induced differentially expressed mRNAs in macrophages were mainly distributed in the essential signaling pathways involved in mitochondrial function and autophagy.The conditioned medium from C_(2)S-treated macrophage robustly promoted osteogenic differentiation in BMSCs.In conclusion,our results indicate mitochondrial dysfunction and autophagy as the possible mechanism of C_(2)S-induced macrophagic inflammation.The promotion of osteogenic differentiation of BMSCs by the C_(2)S-induced macrophagic inflammation suggests the potential application of C_(2)S in developing immunomodulatory bone grafts. | Qianting Luo Xingyang Li Wenchao Zhong Wei Cao Mingjing Zhu Antong Wu Wanyi Chen Zhitong Ye Qiao Han Duraipandy Natarajan Janak L.Pathak Qingbin Zhang | 2022 | Regenerative Biomaterials2022，9，1： | 0 |
| 13 | DISTURBANCE ATTENUATION VIA STATE-FEEDBACK CONTROLLERS显示文摘This paper focuses on the H optimal control problem in which the wholestate is available for feedback. We show that in attenuating the disturbance, the Hoptimal performance of dynamic state-feedback is no better than that of static state-feedback, which generalizes current results for linear time-invariant systems with no directtransmission from the disturbance and control input to the controlled output. | CHEN Wanyi(College of Mathematics,Nankai University, Tianjin 300071, China)TU Fengsheng(Department of Computer and System Science, Nankai University, Tianjin 300071, China) | 1999 | Systems Science and Mathematical Sciences1999，12，4： | 0 |
| 14 | Overview of epigenetic degraders based on PROTAC, molecular glue, and hydrophobic tagging technologies显示文摘Epigenetic pathways play a critical role in the initiation, progression, and metastasis of cancer. Over the past few decades, significant progress has been made in the development of targeted epigenetic modulators(e.g., inhibitors). However, epigenetic inhibitors have faced multiple challenges,including limited clinical efficacy, toxicities, lack of subtype selectivity, and drug resistance. As a result,the design of new epigenetic modulators(e.g., degraders) such as PROTACs, molecular glue, and hydrophobic tagging(Hy T) degraders has garnered significant attention from both academia and pharmaceutical industry, and numerous epigenetic degraders have been discovered in the past decade. In this review,we aim to provide an in-depth illustration of new degrading strategies(2017-2023) targeting epigenetic proteins for cancer therapy, focusing on the rational design, pharmacodynamics, pharmacokinetics, clinical status, and crystal structure information of these degraders. Importantly, we also provide deep insights into the potential challenges and corresponding remedies of this approach to drug design and development. Overall, we hope this review will offer a better mechanistic understanding and serve as a useful guide for the development of emerging epigenetic-targeting degraders. | Xiaopeng Peng Zhihao Hu Limei Zeng Meizhu Zhang Congcong Xu Benyan Lu Chengpeng Tao Weiming Chen Wen Hou Kui Cheng Huichang Bi Wanyi Pan Jianjun Chen | 2024 | Acta Pharmaceutica Sinica B2024，14，2： | 0 |
| 15 | High-resolution genetic mapping and identification of candidate genes for the wheat stem rust resistance gene Sr8155B1显示文摘Stem rust,caused by Puccinia graminis f.sp.tritici(Pgt),threatens global wheat production.Development of cultivars with increased resistance to stem rust by identification,mapping,and deployment of resistance genes is the best strategy for controlling the disease.In this study,we performed fine mapping and characterization of the all-stage stem rust resistance(Sr)gene Sr8155B1 from the durum wheat line 8155-B1.In seedling tests of biparental populations,Sr8155B1 was effective against six Chinese Pgt races tested.In a segregating population of 5060 gametes,Sr8155B1 was mapped to a 0.06-cM region flanked by markers Pku2772 and Pku43365,corresponding to 1.5-and 2.7-Mb regions in the Svevo and Chinese Spring reference genomes.Both regions include several typical nucleotide-binding leucine-rich repeat(NLR)and protein kinase genes that represent candidate genes.Among them,three NLR genes and three receptor-like protein kinases were highly polymorphic between the parental lines and their transcripts were upregulated in the homozygous resistant line TdR2 relative to its susceptible sister line TdS4.Four markers(Pku2772,Pku43365,Pku2950,and Pku3721)developed in this study,together with seedling resistance responses,correctly predicted Sr8155B1 absence or presence in 78 tetraploid wheat genotypes tested.The presence of Sr8155B1 in tetraploid wheat accessions CItr 14916,PI 197492,and PI 197493 was confirmed by mapping in three F_(2)populations.The genetic map and linked markers developed in this study may accelerate the deployment of Sr8155B1-mediated resistance in wheat breeding programs. | Jian Wang Hongyu Li Tao Shen Shikai Lyu Shams ur Rehman Hongna Li Guiping Wang Binyang Xu Qing Wang Wanyi Hu Kairong Li Shengsheng Bai Jian Ma Haitao Yu Matthew N.Rouse Shisheng Chen | 2023 | The Crop Journal2023，11，6： | 0 |
| 16 | Traditional Culture Still Influences Chinese Women显示文摘TRADITIONAL Chinese culture dates back to ancient times. But this culture is the outcome of a patriarchal agricultural society, and is most certainly stamped with the mark of feudalism. During a time when the state and the family had the same structure, women’s problems most typically reflected the characters of the patriarchal clan agricultural society and the traditional culture. | YAN WANYI and CAO DAWEI CHEN SHANSHAN | 1995 | Women of China1995，，5： | 0 |
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