|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Reproductive management through integration of PGD and MPS-based noninvasive prenatal screening/diagnosis for a family with GJB2-associated hearing impairment显示文摘A couple with a proband child of GJB2(encoding the gap junction protein connexin 26)-associated hearing impairment and a previous pregnancy miscarriage sought for a reproductive solution to bear a healthy child. Our study aimed to develop a customized preconception-to-neonate care trajectory to fulfill this clinical demand by integrating preimplantation genetic diagnosis(PGD), noninvasive prenatal testing(NIPT), and noninvasive prenatal diagnosis(NIPD) into the strategy. Auditory and genetic diagnosis of the proband child was carried out to identify the disease causative mutations. The couple then received in-vitro-fertilization treatment, and eight embryos were obtained for day 5 biopsy. PGD was performed by short-tandem-repeat linkage analysis and Sanger sequencing of GJB2 gene. Transfer of a GJB2 c.235del C heterozygous embryo resulted in a singleton pregnancy. At the 13 th week of gestation, genomic DNA(g DNA) from the trio family and cell-free DNA(cf DNA) from maternal plasma were obtained for assessment of fetal chromosomal aneuploidy and GJB2 mutations. NIPT and NIPD showed the absence of chromosomal aneuploidy and GJB2-associated disease in the fetus, which was later confirmed by invasive procedures and postnatal genetic/auditory diagnosis. This strategy successfully prevented the transmission of hearing impairment in the newborn, thus providing a valuable experience in reproductive management of similar cases and potentially other monogenic disorders. | XIONG WenPing WANG DaYong GAO Yuan GAO Ya WANG HongYang GUAN Jing LAN Lan YAN JunHao ZONG Liang YUAN Yuan DONG Wei HUANG SeXin WU KeLiang WANG YaoShen WANG ZhiLi PENG HongMei LU YanPing XIE LinYi ZHAO Cui WANG Li ZHANG QiuJing GAO Yun LI Na YANG Ju YIN ZiFang HAN Bing WANG Wei CHEN Zi-Jiang WANG QiuJu | 2015 | Science China(Life Sciences)2015，58，9： | 12 |
| 2 | A 2-kb Mycovirus Converts a Pathogenic Fungus into a Beneficial Endophyte for Brassica Protection and Yield Enhancement显示文摘Mycoviruses are viruses that infect fungi,and hypovirulence-associated mycoviruses have the potential to control fungal diseases.However,it is unclear how mycovirus-mediated hypovirulent strains live and survive in the field,and no mycovirus has been applied for field crop protection.In this study,we found that a previously identified small DNA mycovirus(SsHADV-1)can convert its host,Sclerotinia sclerotiorum,from a typical necrotrophic pathogen to a beneficial endophytic fungus.SsHADV-1 downregulates the expression of key pathogenicity factor genes in S.sclerotiorum during infection.When growing in rapeseed,the SsHADV-1-infected strain DT-8 significantly regulates the expression of rapeseed genes involved in defense,hormone signaling,and circadian rhythm pathways.As a result,plant growth is promoted and disease resistance is enhanced.Field experiments showed that spraying DT-8 at the early flowering stage can reduce the disease severity of rapeseed stem rot by 67.6%and improve yield by 14.9%.Moreover,we discovered that SsHADV-1 could also infect other S.sclerotiorum strains on DT-8-inoculated plants and that DT-8 could be recovered from dead plants.These findings suggest that the mycoviruses may have the ability to shape the origin of endophytism.Our discoveries suggest that mycoviruses may influence the origin of endophytism and may also offer a novel strategy for disease control in which mycovirus-infected strains are used to improve crop health and release mycoviruses into the field. | Hongxiang Zhang Jiatao Xie Yanping Fu Jiasen Cheng Zheng Qu Zhenzhen Zhao Shufen Cheng Tao Chen Bo Li Qianqian Wang Xinqiang Liu Binnian Tian David B.Collinge Daohong Jiang | 2020 | Molecular Plant2020，13，10： | 9 |
| 3 | Sources and Accumulation Risk of Heavy Metal in Major Animal Manure显示文摘Feed and corresponding manure samples were collected from 27 scale pig farms and 7 scale chicken farms in Jiaxing City,Zhejiang Province,and their heavy metal contents were analyzed. The weighted average values of Cr,Cu,Zn,Pb and As in pig feed were 4. 6,123. 4,370. 7,0. 69 and 6. 9 mg/kg,respectively. However,referred to Safe Use Specification of Feed Additives and MOA Announcement No. 1224,the exceeding standards rates of Cr,Cu,Zn,Pb and As in pig feed were 5. 36%,62. 5%,19. 64%,3. 57% and 37. 5%,respectively. The sequences of heavy metal content in pig feed successively were piglet > growing pig > big pig > sow,but the exceeding standard rates of heavy metal in feed were big pig > sow > growing pig > piglet,and the exceeding standard rate of big pig feed was more than 95%. The exceeding standard rates of Cu,Zn and As in chicken feed were 46. 7%,11. 1% and 33. 3% respectively. Based on Organic Fertilizer( NY525-2012) and Sludge Agricultural Pollution Control Standard( GB4284-84),Cu,Zn and As in pig manure and chicken manure exceeded the standard; the exceeding standard rates of pig manure were 71. 9%,78. 9% and 42. 1%,while that of chicken manure were 12. 5%,25. 0% and 44. 4%,respectively. The exceeding standard rates of Cu and Zn in piglet manure reached 100%. There were very significant positive correlations in Cu,Zn and As between pig feed and corresponding manure,or Hg,Cu and As between chicken feed and corresponding manure,and the correlation coefficients r^2 were greater than 0. 8. Besides,the correlation coefficient r^2 of Pb between pig feed and corresponding manure was above 0. 6. Continuous and high intensity of livestock manure application significantly promoted the accumulation of heavy metal in farmland soil,but the effects on heavy metal accumulation in rice was not significant. | Shi Yanping Huang Jinfa Ni Xiongwei Xie Jianping Ren Jiajia | 2016 | Animal Husbandry and Feed Science2016，8，6： | 4 |
| 4 | Fast approximate matching of binary codes with distinctive bits显示文摘 | Chenggang Clarence YAN Hongtao XIE Bing ZHANG Yanping MA Qiong DAI Yizhi LIU | 2015 | Frontiers of Computer Science2015，9，5： | 3 |
| 5 | Development of Cu foam-based Ni catalyst for solar thermal reforming of methane with carbon dioxide显示文摘Using solar energy to produce syngas via the endothermic reforming of methane has been extensively investigated at the laboratory- and pilot plant-scales as a promising method of storing solar energy. One of the challenges to scaling up this process in a tubular reformer is to improve the reactor's performance, which is limited by mass and heat transfer issues. High thermal conductivity Cu foam was therefore used as a substrate to improve the catalyst's thermal conductivity during solar reforming. We also developed a method to coat the foam with the catalytically active component NiMg_3AlO_x. The Cu foam-based NiMg_3AlO_x performs better than catalysts supported on SiSiC foam, which is currently used as a substrate for solar-reforming catalysts, at high gas hourly space velocity(≥400,000 mL/(g·h)) or at low reaction temperatures(≤ 720 °C). The presence of a γ-Al_2O_3 intermediate layer improves the adhesion between the catalyst and substrate as well as the catalytic activity. | Jianzhong Qi Yanping Sun Zongli Xie Mike Collins Hao Du Tianying Xiong | 2015 | Journal of Energy Chemistry2015，24，6： | 3 |
| 6 | Structural basis of ALMT1-mediated aluminum resistance in Arabidopsis显示文摘The plant aluminum(Al)-activated malate transporter ALMT1 mediates the efflux of malate to chelate the Al in acidic soils and underlies the plant Al resistance.Here we present cryo-electron microscopy(cryo-EM)structures of Arabidopsis thaliana ALMT1(AtALMT1)in the apo,malate-bound,and Al-bound states at neutral and/or acidic pH at up to 3.0Åresolution.The AtALMT1 dimer assembles an anion channel and each subunit contains six transmembrane helices(TMs)and six cytosolicα-helices.Two pairs of Arg residues are located in the center of the channel pore and contribute to malate recognition.Al binds at the extracellular side of AtALMT1 and induces conformational changes of the TM1-2 loop and the TM5-6 loop,resulting in the opening of the extracellular gate.These structures,along with electrophysiological measurements,molecular dynamic simulations,and mutagenesis study in Arabidopsis,elucidate the structural basis for Al-activated malate transport by ALMT1. | Jiangqin Wang Xiafei Yu Zhong Jie Ding Xiaokang Zhang Yanping Luo Ximing Xu Yuan Xie Xiaoxiao Li Tian Yuan Shao Jian Zheng Wei Yang Jiangtao Guo | 2022 | Cell Research2022，32，1： | 3 |
| 7 | Crack initiation and propagation induced by inclusions in a nickel-base P/M superalloy under fatigue load显示文摘In situ fatigue tests in special designed SEM were conducted to trace the whole process of crack initiation andpropagation till to fracture in nickel-base P/M superalloy seeded inclusions. The experimental results show that non-metallicinclusions can induce crack initiation. When the inclusion size is larger than the critical one, the crack can propagate as themain crack that induces the specimen to fracture. As a result, the LCF life of the specimen decreases. | ZENG Yanping ZHANG Maicang DONG Jianxin ZHANG Lina XIE Xishan | 2005 | Rare Metals2005，24，3： | 2 |
| 8 | Formulation of Organic Fertilizer Standard in Jiaxing City Based on NY525-2012显示文摘On the basis of research, analysis and argumentation, we conduct analysis and research on the current situation of quality of commercial organic fertilizer in Jiaxing City, and carry out the inspection and testing of organic matter indicators using the laboratory. Based on the standard of NY525-2012 Organic Fertilizer by the Ministry of Agriculture, this paper formulates the quality level indicators of local commercial organic fertilizer and adding specifications of raw materials. | Jinfa HUANG Xiongwei NI Jianping XIE Yanping SHI | 2013 | Asian Agricultural Research2013，5，4： | 2 |
| 9 | Mechanisms of biochar decreasing methane emission from Chinese paddy soils显示文摘 | Youzhi Feng Yanping Xu Yongchang Yu Zubin Xie Xiangui Lin | 2011 | Soil Biology and Biochemistry2011，，： | 2 |
| 10 | SEM in-situ investigation on fatigue cracking behavior of P/M Rene95 alloy with surface inclusions显示文摘The low-cycle fatigue behavior of powder metallurgy Rene95 alloy containing surface inclusions was investigated by in-situ observation with scanning electron microscopy (SEM). The process of fatigue crack initiation and early stage of propagation behavior indicates that fatigue crack mainly occurs at the interface between the inclusion and the matrix. The effect of inclusion on the fatigue crack initiation and the early stage of crack growth was very obvious. The fatigue crack growth path in the matrix is similar to the shape of inclusion made on the basis of fatigue fracture image analysis. The empiric relation between the surface and inside crack growth length, near a surface inclusion, can be expressed. Therefore, the fatigue crack growth rate or life of P/M Rene95 alloy including the inclusions can be evaluated on the basis of the measurable surface crack length parameter. In addition, the effect of two inclusions on the fatigue crack initiation behavior was investigated by the in-situ observation with SEM. | Xishu Wang Lina Zhang Yanping Zeng Xishan Xie | 2006 | Journal of University of Science and Technology Beijing2006，13，3： | 2 |
| 11 | A durable 4-1BB-based CD19 CAR-T cell for treatment of relapsed or refractory non-Hodgkin lymphoma显示文摘Objective:Previous studies reported that 4-1BB-based CD19 chimeric antigen receptor(CAR)-T cells were more beneficial for the clinical outcomes than CD28-based CAR-T cells,especially the lower incidence rate of severe adverse events.However,the median progression-free survival(mPFS)of 4-1BB-based product Kymriah was shorter than that of CD28-based Yescarta(2.9 months vs.5.9 months),suggesting that Kymriah was limited in the long-term efficacy.Thus,a safe and durable 4-1BB-based CD19 CAR-T needs to be developed.Methods:We designed a CD19-targeted CAR-T(named as IM19)which consisted of an FMC63 scFv,4-1BB and CD3ζintracellular domain and was manufactured into a memory T-enriched formulation.A phase I/II clinical trial was launched to evaluate the clinical outcomes of IM19 in relapsed or refractory(r/r)B cell non-Hodgkin lymphoma(B-NHL).Dose-escalation investigation(at a dose of 5×10^(5)/kg,1×10^(6)/kg and 3×106/kg)was performed in 22 r/r B-NHL patients.All patients received a single infusion of IM19 after 3-day conditional regimen.Results:At month 3,the overall response rate(ORR)was 59.1%,the complete response rate(CRR)was 50.0%.The mPFS was 6 months and the 1-year overall survival rate was 77.8%.Cytokine release syndrome(CRS)occurred in 13 patients(59.1%),with 54.5%of grade 1−2 CRS.Only one patient(4.5%)experienced grade 3 CRS and grade 3 neurotoxicity.Conclusions:These results demonstrated the safety and durable efficacy of a 4-1BB-based CD19 CAR-T,IM19,which is promising for further development and clinical investigation. | Zhitao Ying Ting He Shanzhao Jin Xiaopei Wang Wen Zheng Ningjing Lin Meifeng Tu Yan Xie Lingyan Ping Weiping Liu Lijuan Deng Yanping Ding Xuelian Hu Bing Bu Xin’an Lu Yuqin Song Jun Zhu | 2022 | Chinese Journal of Cancer Research2022，34，1： | 2 |
| 12 | Preparation and Determination of Immunological Activities of Anti-HBV Egg Yolk Extraction显示文摘准备有效有免疫力的准备对待肝炎 B,母鸡与肝炎 B 疫苗,然后 anti-HBV 卵蛋黄被使免疫抽取(anti-HBV 眼睛) 被一个 dialyzable 方法从卵蛋黄精制。它的化学特征被紫外光谱, HPLC, Lowry 分析和 pharmacopocia-raleted 方法识别。特定的免疫学的活动被白血球坚持抑制(LAI ) 在 vitro 检验并且在 vivo 推迟了类型超敏性(DTH ) 。Anti-HBV 眼睛是有分子的重量的一种小 dialyzable 物质包含 18 种氨基酸的不到 12 kD。准备能显然禁止类似于猪怒气的肝炎 B 病毒特定的转移因素的 LAI 和 DTH。然而,没有类似的效果,在 nonspecific 转移因素(NTF ) 观察组,控制鸡蛋蛋黄抽取(CEYE ) 组和肝炎一个病毒(HAV ) 组。结果建议那只 anti-HBV 眼睛包含了肝炎 B 病毒特定的转移因素(STF ) 并且举办了类似于 PSHBV-TF 的抗原特定的房间免疫者活动。从母鸡的卵蛋黄获得的 STF 与特定的抗原使免疫,可能是在肝炎 B 预防和治疗的 immunoregulation 的一个潜在的候选人。 | Yanping Xu Weimin Zou Xuejun Zhan Shuhua Yang Daze Xie Sailiang Peng | 2006 | Cellular & Molecular Immunology2006，3，1： | 2 |
| 13 | Copper-Catalyzed Aerobic Oxidative Amination of Arylboronic Acid with Aminal under Base-Free Conditions显示文摘 | Yanping Zhou Yinjun Xie Lei Yang Pan Xie Hanmin Huang | 2013 | Tetrahedron Letters2013，，21： | 1 |
| 14 | New Quinoxaline-Based Blue Emitters: Molecular Structures, Aggregation-Induced Enhanced Emission Characteristics and OLED Application显示文摘Three new deep blue light emitters,bearing quinoxaline with different substituents(biphenyl/pyrene)at the 1-or 2-position,were synthesized by a Pd-catalysed coupling reaction in high yields,and were fully characterized by 1H/13C NMR spectroscopy,single crystal X-ray diffraction,and high-resolution mass spectrometry(HRMS). | Xiaoyu Mao Fuli Xie Xiaohui Wang Qingsong Wang Zhipeng Qiu Mark R.J.Elsegood Jie Bai Xing Feng Carl Redshaw Yanping Huo Jian-Yong Hu Qing Chen | 2021 | Chinese Journal of Chemistry2021，39，8： | 1 |
| 15 | Magnetic molecularly imprinted polymer for the detection of rhaponticin in Chinese patent medicines显示文摘 | Chen Fangfang Xie Xiaoyu Shi Yanping | 2012 | Journal of Chromatography A2012，1252，： | 1 |
| 16 | Molecular typing of Vibrio parahaemolyticus isolates from the middle-east coastline of China显示文摘 | CHEN Wanyi XIE Yanping XU Jingye | 2012 | International Journal of Food Microbiology2012，153，3： | 1 |
| 17 | Scriptaid affects histone acetylation and the expression of development-related genes at different stages of porcine somatic cell nuclear transfer embryo during early development显示文摘Although the somatic cell nuclear transfer(SCNT) technique has been used extensively for cloning and generating transgenic pigs,the cloning efficiency is still very low.It has been proposed that the low efficiency of this technique is the result of incomplete epigenetic reprogramming and abnormal gene expression during early embryonic development.In this study,we investigate the effect of Scriptaid,a low-toxicity histone deacetylase inhibitor,on the developmental competence of porcine SCNT embryos.We found that treating SCNT embryos with 500 nmol/L Scriptaid for 15 h after activation significantly enhanced the blastocyst formation rate(27.7%) compared with the untreated group(control)(12.2%,P<0.05).Using an immunofluorescence technique to measure the average fluorescence intensity,we also found that treating SCNT embryos with Scriptaid increased the level of histone acetylation on histone H3 at lysine 14(acH3K14).Furthermore,treating embryos with Scriptaid increased the expression level of three genes that play important roles during embryonic development(Oct4,Klf4 at the blastocyst stage and Nanog at the 4-cell stage).Moreover,the expression level of the apoptosis-related gene Caspase-3 was significantly lower in the Scriptaid-treated SCNT embryos compared with the control SCNT embryos at the 4-cell and blastocyst stages.In conclusion,these results indicate that Scriptaid treatment improves the development and nuclear reprogramming of porcine SCNT embryos. | ZHOU Yan HUANG YongYe XIE WanHua SONG Qi JI Yuan ZHANG YanPing OUYANG HongSheng LAI LiangXue PANG DaXin TANG XiaoChun | 2013 | Chinese Science Bulletin2013，58，18： | 1 |
| 18 | Genome-wide analysis of primary auxin-responsive Aux/IAA gene family in maize (Zea mays. L.)显示文摘 | Yijun Wang Dexiang Deng Yunlong Bian Yanping Lv Qin Xie | 2010 | Molecular Biology Reports2010，，8： | 1 |
| 19 | Mechanisms of biochar decreasing methane emission from Chinese paddy soils显示文摘 | Youzhi Feng Yanping Xu Yongchang Yu Zubin Xie Xiangui Lin | 2011 | Soil Biology and Biochemistry2011，，： | 1 |
| 20 | Antifungal substances produced by Penicillium oxalicum strain PY-1—potential antibiotics against plant pathogenic fungi显示文摘 | Liping Yang Jiatao Xie Daohong Jiang Yanping Fu Guoqing Li Fangcan Lin | 2008 | World Journal of Microbiology and Biotechnology2008，，7： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员