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| 1 | Novel KCl/Silicate Drilling Fluids for Alleviating Problems in Troublesome Shale Formations in Sudan显示文摘为几十年,井在块 6 钻了,苏丹,经历了与由于在 Aradeiba 和 Abu Gabra 形成存在的高度反应、散的页岩钻液体有关的严肃的洞不稳定性问题。这些问题包括了洞节与紧密的洞,以及严肃的 sloughing 相结合的冲刷。经常的擦拭器旅行井被要求并且记载不通常是成功的。以前,几常规禁止的以水为基的钻液体象 KCl/polymer, KCl/lime/polymer 和 KCl/PHPA 那样(部分 hydrolyzed polyacrylamide ) 在这个区域被使用了,但是与在洞稳定性和钻的性能的仅仅边缘的改进。最近,一个最新提出的 KCl /sodium 硅酸盐系统,被性质和过滤控制的好 rheological 描绘,被开发并且使用了提供必要禁止的字符。第一试用很好与这种钻被钻在块 6 的液体,苏丹,和下列受益被观察:(1 ) 优秀正直为页岩形成由钻的剪样展出了;(2 ) 稳定的地上凿穿与更低的泥重量保留了;(3 ) 减少了擦拭器旅行;并且(4 ) 从 caliper logs.Later 显示出在上的在里面计量器地上凿穿,五口更多的井在这个区域与 KCl /sodium 硅酸盐系统被钻。KCl/sodium 硅酸盐系统罐头充分遇见在这个区域钻操作的要求,这被发现。案例研究以擦拭器旅行,剪样的禁止的特性,洞条件和泥重量在这份报纸被介绍。 | Guo Jiankang Yan Jienian Fan Weiwang Zhang Hongjing Wang Qunli Yu Zhihai | 2005 | Petroleum Science2005，2，4： | 2 |
| 2 | Production of transgenic pigs over-expressing theantiviral gene Mx1显示文摘The myxovirus resistance gene (Mx1) has a broad spectrum of antiviral activities. It is therefore an interestingcandidate gene to improve disease resistance in farm animals. In this study, we report the use of somatic cellnuclear transfer (SCNT) to produce transgenic pigs over-expressing the Mx1 gene. These transgenic pigs expressapproximately 15–25 times more Mx1 mRNA than non-transgenic pigs, and the protein level of Mx1 was alsomarkedly enhanced. We challenged fibroblast cells isolated from the ear skin of transgenic and control pigs withinfluenza A virus and classical swine fever virus (CFSV). Indirect immunofluorescence assay (IFA) revealed a profounddecrease of influenza A proliferation in Mx1 transgenic cells. Growth kinetics showed an approximately 10-foldreduction of viral copies in the transgenic cells compared to non-transgenic controls. Additionally, we found thatthe Mx1 transgenic cells were more resistant to CSFV infection in comparison to non-transgenic cells. These resultsdemonstrate that the Mx1 transgene can protect against viral infection in cells of transgenic pigs and indicate thatthe Mx1 transgene can be harnessed to develop disease-resistant pigs. | Quanmei Yan Huaqiang Yang Dongshan Yang Bentian Zhao Zhen Ouyang Zhaoming Liu Nana Fan Hongsheng Ouyang Weiwang Gu Liangxue Lai | 2014 | Cell Regeneration2014，3，1： | 2 |
| 3 | Applications of strongly inhibitive silicate-based drilling fluids introublesome shale formations in Sudan 显示文摘 | Jiankang Guo Jienian Yan Weiwang Fan Hongiing Zhang | 2006 | Journal of Petroleum Science and Engineering2006，50，： | 1 |
| 4 | Optimization and standardization of transient expression assays for gene functional analyses in strawberry fruits显示文摘Strawberry is increasingly used as a model plant for research on fruit growth and development.The transient gene manipulation(TGM)technique is widely used to determine the function of plant genes,including those in strawberry fruits.However,its reliable application for the precise identification of gene function has been difficult owing to the lack of conditional optimization.In this study,we found that successful transient gene manipulation requires optimization,with the vector type,temperature,and fruit developmental stage being three major factors determining success.Notably,we found that transient gene manipulation was feasible only from the large green fruit stage onwards,making it especially suitable for identifying genes involved in strawberry fruit ripening.Furthermore,we established a method called percentage difference of phenotype(PDP),in which the functional effect of a gene could be precisely and efficiently identified in strawberry fruits.This method can be used to estimate the functional effect of a gene as a value from 0 to 100%,such that different genes can be quantitatively compared for their relative abilities to regulate fruit ripening.This study provides a useful tool for accelerating research on the molecular basis of strawberry fruit ripening. | Yaoyao Zhao Wenwen Mao Yating Chen WeiWang Zhengrong Dai Zhechao Dou Kai Zhang Lingzhi Wei Tianyu Li Baozhen Zeng Ting Liu Yijuan Fan Jiaqi Yan Bingbing Li Wensuo Jia | 2019 | Horticulture Research2019，6，1： | 1 |
| 5 | Application of Strongly Inhibitive Silicate-based Drilling Fluids in Troublesome Shale Formation in Sudan显示文摘 | Guo Jiankang Yah Jienian Fan Weiwang | 2006 | Journal of Petroleum Science and Enginerring2006，50，34： | 1 |
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