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| 1 | Piglets cloned from induced pluripotent stem cells显示文摘 | Nana Fan Jijun Chen Zhouchun Shang Hongwei Dou Guangzhen Ji Qingjian Zou Lu Wu Lixiazi He Fang Wang Kai Liu Na Liu Jianyong Han Qi Zhou Dengke Pan Dongshan Yang Bentian Zhao Zhen Ouyang Zhaoming Liu Yu Zhao Lin Lin Chongming Zhong Quanlei Wang Shouqi Wang Ying Xu Jing Luan Yu Liang Zhenzhen Yang Jing Li Chunxia Lu Gabor Vajta Ziyi Li Hongsheng Ouyang Huayan Wang Yong Wang Yang Yang Zhonghua Liu Hong Wei Zhidong Luan Miguel A Esteban Hongkui Deng Huanming Yang Duanqing Pei Ning Li Gang Pei Lin Liu Yutao Du Lei Xiao Liangxue Lai | 2013 | Cell Research2013，23，1： | 12 |
| 2 | Genetically Modified Pig Models for Human Diseases显示文摘Genetically modified animal models are important for understanding the pathogenesis of human disease and developing therapeutic strategies.Although genetically modified mice have been widely used to model human diseases,some of these mouse models do not replicate important disease symptoms or pathology.Pigs are more similar to humans than mice in anatomy,physiology,and genome. Thus,pigs are considered to be better animal models to mimic some human diseases.This review describes genetically modified pigs that have been used to model various diseases including neurological,cardiovascular,and diabetic disorders.We also discuss the development in gene modification technology that can facilitate the generation of transgenic pig models for human diseases. | Nana Fan Liangxue Lai | 2013 | Journal of Genetics and Genomics2013，40，2： | 11 |
| 3 | Calcium carbonate-methylene blue nanohybrids for photodynamic therapy and ultrasound imaging显示文摘Photodynamic therapy plays an important role in cancer treatment.In this work,methylene blue(MB)-embedded calcium carbonate nanorods(CaCO_3-MB NRs)have been synthesized for p H-responsive photodynamic therapy and ultrasound imaging.The morphology of CaCO_3-MB NRs can be controlled by modulating the concentration of Na_2CO_3 aqueous solution.The generation of effective reactive oxygen species(ROS)were confirmed by 1,3-diphenylisobenzofuran(DPBF)probe.Both photodynamic therapy performance and echogenic performance of CaCO_3-MB NRs were investigated to confirm the feasibility of CaCO_3-MB nanohybrids for ultrasound image-guided photodynamic therapy. | Weili Fan Yu Qi Ranran Wang Chen Xu Nana Zhao Fu-Jian Xu | 2018 | Science China(Life Sciences)2018，61，4： | 8 |
| 4 | Genetically humanized pigs exclusively expressing human insulin are generated through custom endonuclease-mediated seamless engineering显示文摘Dear Editor,Type1diabetes(T1D)isa lifelong(chronic)disease and a major health problem throughout the world.This disease can be treatedby either insulin injection or islet transplantation.Islet transplantation is considered as a better treatment for T1D patients,because islets can produce and release insulin at the appropriate time,resulting in tight blood glucose control. | Yi Yang Kepin Wang Han Wu Qin Jin Degong Ruan Zhen Ouyang Bentian Zhao Zhaoming Liu Yu Zhao Quanjun Zhang Nana Fan Qishuai Liu Shimei Guo Lei Bu Yong Fan Xiaofang Sun Xiaoping Li Liangxue Lai | 2016 | Journal of Molecular Cell Biology2016，8，2： | 6 |
| 5 | Theoretical investigation on structures,electronic spectra and nonlinear optical properties of gold compounds [X-{Au(PMe_3)}_2]显示文摘The structures,electronic spectra,polarizability and third-order nonlinear optical properties of six gold compounds [X-{Au(PMe3)}2]were investigated by density functional theory(DFT)B3LYP and BhandHLYP methods.It was found that the calculation methods and basis set are rational for the object of study,and molecular structures change slightly when PPh3 is replaced by PMe3.The spatial effects of the bridging section have a significant influence on the polarizability,but indistinctive to the third-order nonlinear optical(NLO)coefficient.As a result of the conjugated effect in different compounds,the third-order polarizability of molecule 1a is the smallest,while that of molecule 2a is the largest.Au has donor ability in molecule 1a but acceptor ability in molecules 2a-6a by analyzing the electronic spectra and frontier molecular orbitals constitute maximal absorption,which indicates the contribution of Au to NLO properties in the six molecules is different. | MA NaNa,QIU YongQing,SUN ShiLing,LIU ChunGuang,FAN Min & SU ZhongMin Institute of Functional Material Chemistry,Faculty of Chemistry,Northeast Normal University,Changchun 130024,China | 2010 | Science China Chemistry2010，53，5： | 3 |
| 6 | Generation of rat blood vasculature and hematopoietic cells in rat-mouse chimeras by blastocyst complementation显示文摘Interspecies chimera through blastocyst complementation could be an alternative approach to create human organs in animals by using human pluripotent stem cells.A mismatch of the major histocompatibility complex of vascular endothelial cells between the human and host animal will cause graft rejection in the transplanted organs.Therefore,to achieve a transplantable organ in animals without rejection,creation of vascular endothelial cells derived from humans within the organ is necessary.In this study,to explore whether donor xeno-pluripotent stem cells can compensate for blood vasculature in host animals,we generated rat-mouse chimeras by injection of rat embryonic stem cells(rESCs)into mouse blastocysts with deficiency of Flk-1 protein,which is associated with endothelial and hematopoietic cell development.We found that rESCs could differentiate into vascular endothelial and hematopoietic cells in the rat-mouse chimeras.The whole yolk sac(YS)of Flk-1^EGFP/ECFP rat-mouse chimera was full of rat blood vasculature.Rat genes related to vascular endothelial cells,arteries,and veins,blood vessels formation process,as well as hematopoietic cells,were highly expressed in the YS.Our results suggested that rat vascular endothelial cells could undergo proliferation,migration,and self-assembly to form blood vasculature and that hematopoietic cells could differentiate into B cells,T cells,and myeloid cells in rat-mouse chimeras,which was able to rescue early embryonic lethality caused by Flk-1 deficiency in mouse. | Xiaomin Wang Hui Shi Juanjuan Zhou Qingjian Zou Quanjun Zhang Shixue Gou Pengfei Chen Lisha Mou Nana Fan Yangyang Suo Zhen Ouyang Chengdan Lai Quanmei Yan Liangxue Lai | 2020 | Journal of Genetics and Genomics2020，47，5： | 2 |
| 7 | Timing of formation of the Hongdonggou Pb-Zn polymetallic ore deposit,Henan Province,China:Evidence from Rb-Sr isotopic dating of sphalerites显示文摘The Hongdonggou Pb-Zn polymetallic ore deposit,located in the southwestern part of the Luanchuan Mo-W-Pb-Zn-Ag polymetallic ore mineralization in Henan Province,China,is an important part of the East Qinling metallogenic belt.The orebodies in the deposit,which are vein,bedded and lenticular,are mainly hosted in the syenite porphyry,and formed within the carbonate and clastic rocks of the Yuku and Qiumugou formations partially.The genesis of the deposit has previously been argued to be of hydrothermal-vein type or of skarn-hydrothermal type.In this study,we report the results of Rb-Sr isotopic dating based on sphalerites from the main orebody of the Hongdonggou Pb-Zn polymetallic ore deposit,which yield an isochron age of 135.7 ± 3.2 Ma,constraining the timing of mineralization as early Cretaceous.The age is close to those reported for the Pb-Zn deposits in the Luanchuan ore belt.The(^(87)Sr/^(86)Sr),values of the sphalerites(0.71127 + 0.00010) are lower than that of terrigenous silicates(0.720) and higher than the mantle(0.707),suggesting that the metallogenic components were mainly derived through crust-mantle mixing.Combining the results from this study with those from previous work,we propose that the Hongdonggou Pb-Zn polymetallic ore deposit is a hydrothermal-vein deposit associated with the early Cretaceous tectonothermal event,and the mineralization is controlled by NWand near EW-trending faults in the Luanchuan Mo-W-Pb-Zn-Ag polymetallic ore concentration belt. | Fan Yang Gongwen Wang Huawen Cao Ruixi Li Li Tang Yufeng Huang Hao Zhang Fei Xue Wenjuan Jia Nana Guo | 2017 | Geoscience Frontiers2017，8，3： | 2 |
| 8 | Production of transgenic pigs over-expressing theantiviral gene Mx1显示文摘The myxovirus resistance gene (Mx1) has a broad spectrum of antiviral activities. It is therefore an interestingcandidate gene to improve disease resistance in farm animals. In this study, we report the use of somatic cellnuclear transfer (SCNT) to produce transgenic pigs over-expressing the Mx1 gene. These transgenic pigs expressapproximately 15–25 times more Mx1 mRNA than non-transgenic pigs, and the protein level of Mx1 was alsomarkedly enhanced. We challenged fibroblast cells isolated from the ear skin of transgenic and control pigs withinfluenza A virus and classical swine fever virus (CFSV). Indirect immunofluorescence assay (IFA) revealed a profounddecrease of influenza A proliferation in Mx1 transgenic cells. Growth kinetics showed an approximately 10-foldreduction of viral copies in the transgenic cells compared to non-transgenic controls. Additionally, we found thatthe Mx1 transgenic cells were more resistant to CSFV infection in comparison to non-transgenic cells. These resultsdemonstrate that the Mx1 transgene can protect against viral infection in cells of transgenic pigs and indicate thatthe Mx1 transgene can be harnessed to develop disease-resistant pigs. | Quanmei Yan Huaqiang Yang Dongshan Yang Bentian Zhao Zhen Ouyang Zhaoming Liu Nana Fan Hongsheng Ouyang Weiwang Gu Liangxue Lai | 2014 | Cell Regeneration2014，3，1： | 2 |
| 9 | Effects of protein oxidation, cathepsins, and various freezing temperatures on the quality of superchilled sturgeon fillets显示文摘Many aquatic products have been stored using superchilling technology, but rarely used for the storage of sturgeon fillets. In this study, we investigated the effects of protein oxidation, cathepsin, and various freezing temperatures on the quality of superchilled sturgeon fillets. Sensory evaluation results showed that the sensory attributes of superchilled (−3 °C) sturgeon fillets were acceptable three times longer (18 days) than samples stored at refrigeration temperatures (4 °C). The sarcoplasmic protein, carbonyl, myofibrillar protein, total sulfhydryl content and the surface hydrophobicity were determined using fluorescence spectrophotometry and SDS-PAGE. Results showed that superchilling might protect myofibrillar proteins from oxidation compared to refrigeration temperatures. The activity of the three cathepsins (B, L, and H) in terms of myofibrillar, mitochondria, lysosomes, and sarcoplasm demonstrated that superchilling can inhibit cathepsins activity in sturgeon and protect its muscle structure. Microscopic observations showed that as the temperature decreased, the gap area of the muscle fibers decreased, and the deformation of cross-sectional slices was gradually reduced. In addition, the freezing rate of ice crystals produced during the freezing process influenced the muscle structure, texture, and sensory attributes. Superchilled sturgeon fillets showed good hardness, chewiness, and water retention. In conclusion, superchilling technology shows promise for its ability to extend the shelf life while maintaining the texture and sensory attributes of fresh sturgeon fillets. | Nana Zhao Xianqing Yang Yujin Li Haohao Wu Yiping Chen Ruichang Gao Feng Xiao Fan Bai Jinlin Wang Zunying Liu Xin Gao Yuanhui Zhao | 2022 | Marine Life Science & Technology2022，4，1： | 1 |
| 10 | Small molecular compounds that inhibit hepatitis C virus replication through destabilizing heat shock cognate 70 messenger RNA显示文摘 | Peng Zonggen Fan Bo Du Nana | 2010 | Hepatology2010，52，3： | 1 |
| 11 | Generation of gene-target dogs using CRISPR/Cas9 system显示文摘Dear Editor,Dogs(Canis familiaris)serve as human companions and are raised to herd livestock,aid hunters,guard homes,perform police and rescue work,and guide the blind.Dogs exhibit close similarities to humans in terms of metabolic,physiological,and anatomical characteristics,and thus are ideal genetic and clinical models to study human diseases(Tsai et al.,2007).Gene target technology is a powerful tool to create new strains of animals with favorable traits.However,thus far,gene-target dogs have not been developed due to their unique species-specific reproductive characteristics,which limits the applications of dogs especially in the field of biomedical research.Recently,clustered regularly interspaced short palindromic repeats(CRISPRs)/CRISPR-associated(Cas)9 system was applied to edit specific genes with a high efficiency(Cong et al.,2013;Mali et al.,2013).Here we attempt to explore the feasibility of producing gene knockout(KO)dogs by using this technology.Beagle dog,the most widely used breed in biomedical research,was used as our animal model.Myostatin(MSTN)was chosen as the first gene of interest. | Qingjian Zou Xiaomin Wang Yunzhong Liu Zhen Ouyang Haibin Long Shu Wei Jige Xin Bentian Zhao Sisi Lai Jun Shen Qingchun Ni Huaqiang Yang Huilin Zhong Li Li Minhua Hu Quanjun Zhang Zhidong Zhou Jiaxin He Quanmei Yan Nana Fan Yu Zhao Zhaoming Liu Lin Guo Jiao Huang Guanguan Zhang Jun Ying Liangxue Lai Xiang Gao | 2015 | Journal of Molecular Cell Biology2015，7，6： | 1 |
| 12 | IRE1α regulates the PTHrP-IHH feedback loop to orchestrate chondrocyte hypertrophy and cartilage mineralization显示文摘Cartilage development is controlled by the highly synergistic proliferation and differentiation of growth plate chondrocytes,in which the Indian hedgehog(IHH)and parathyroid hormone-related protein-parathyroid hormone-1 receptor(PTHrP-PTH1R)feedback loop is crucial.The inositol-requiring enzyme 1a/X-box-binding protein-1 spliced(IRE1α/XBP1s)branch of the unfolded protein response(UPR)is essential for normal cartilage development.However,the precise role of ER stress effector IRE1α,encoded by endoplasmic reticulum to nucleus signaling 1(ERN1),in skeletal development remains unknown.Herein,we reported that loss of IRE1α accelerates chondrocyte hypertrophy and promotes endochondral bone growth.ERN1 acts as a negative regulator of chondrocyte proliferation and differentiation in postnatal growth plates.Its deficiency interrupted PTHrP/PTH1R and IHH homeostasis leading to impaired chondrocyte hypertrophy and differentiation.XBP1s,produced by p-IRE1α-mediated splicing,binds and up-regulates PTH1R and IHH,which coordinate cartilage development.Meanwhile,ER stress cannot be activated normally in ERN1-deficient chondrocytes.In conclusion,ERN1 deficiency accelerates chondrocyte hypertrophy and cartilage mineralization by impairing the homeostasis of the IHH and PTHrP/PTH1R feedback loop and ER stress.ERN1 may have a potential role as a new target for cartilage growth and maturation. | Mengtian Fan Nana Geng Xingyue Li Danyang Yin Yuyou Yang Rong Jiang Cheng Chen Naibo Feng Li Liang Xiaoli Li Fengtao Luo Huabing Qi Qiaoyan Tan Yangli Xie Fengjin Guo | 2024 | Genes & Diseases2024，11，1： | 0 |
| 13 | Duality of the fatigue behavior and failure mechanism in notched specimens of Ti-7Mo-3Nb-3Cr-3Al alloy显示文摘An interesting phenomenon of dual S-N fatigue behavior is investigated in a metastable β titanium alloy,Ti-7 Mo-3 Nb-3 Cr-3 Al notched cylindrical specimens with an elastic stress concentration factor of Kt=3.Fractographic studies revealed all specimens,and irrespective of lifetime,failed from the specimen surface because of stress concentration occurs at the notch root.Typically,the short-life-distribution is usually associated with surface-failure-without-facets and the long-life-distribution generally occurs due to surface-failure-with-facets.This competing failure leads to increasing the variability in fatigue lifetime and further facilitates the difficulty in prediction of fatigue lifetime.Crack-initiation area characterization was conducted by using mechanical grinding,focused ion beam milling and subsequent electron backscattered diffraction(EBSD) analysis of the 2 D section across faceted grains.Results show that the α_p particles(especially the elongated α_p particles) well-oriented for basal slip activation is a preferential fatigue-critical microstructural configuration.Additionally,the β+α_s matrix has a higher KAM value than the α_p particles in fatigued microstructures and significant dislocation activity in the form of dislocation tangles is observed in α_p boundaries. | Zhihong Wu Hongchao Kou Nana Chen Mengqi Zhang Ke Hua Jiangkun Fan Bin Tang | 2020 | Journal of Materials Science & Technology2020，47，15： | 0 |
| 14 | Iridium complex of porphycene:a new member of metalloporphycene显示文摘Iridium(Ⅲ)porphycene complex was synthesized and structurally characterized for the first time.The introduction of the posttransition iridium(Ⅲ)ion not only significantly enhances the absorption intensity at the end of visible region,but also displays efficient singlet oxygen quantum yield(76%),which is applicable for photodynamic therapy in living cells. | Yi Chang Fei Zhao Fan Wu Nana Ma Xiaoming Ma Yue Zhao Kin Shing Chan Zhen Shen | 2020 | Science China Chemistry2020，63，5： | 0 |
| 15 | The divergence of brassinosteroid sensitivity between rice subspecies involves natural variation conferring altered internal auto-binding of OsBSK2显示文摘Japonica/geng and indica/xian are two major rice(Oryza sativa)subspecies with multiple divergent traits,but how these traits are related and interact within each subspecies remains elusive.Brassinosteroids(BRs)are a class of steroid phytohormones that modulate many important agronomic traits in rice.Here,using different physiological assays,we revealed that japonica rice exhibits an overall lower BR sensitivity than indica.Extensive screening of BR signaling genes led to the identification of a set of genes distributed throughout the primary BR signaling pathway with divergent polymorphisms.Among these,we demonstrate that the C38/T variant in BR Signaling Kinase2(OsBSK2),causing the amino acid change P13L,plays a central role in mediating differential BR signaling in japonica and indica rice.OsBSK2in indica plays a greater role in BR signaling than OsB SK2in japonica by affecting the auto-binding and protein accumulation of OsBSK2.Finally,we determined that OsBSK2 is involved in a number of divergent traits in japonica relative to indica rice,including grain shape,tiller number,cold adaptation,and nitrogen-use efficiency.Our study suggests that the natural variation in OsB SK2 plays a key role in the divergence of BR signaling,which underlies multiple divergent traits between japonica and indica. | Wenchao Yin Lulu Li Zhikun Yu Fan Zhang Dapu Liu Hongkai Wu Mei Niu Wenjing Meng Xiaoxing Zhang Nana Dong Yanzhao Yang Jihong Liu Yongqiang Liu Guoxia Zhang Jianlong Xu Shimei Wang Chengcai Chu Qian Qian Hongning Tong | 2022 | Journal of Integrative Plant Biology2022，64，8： | 0 |
| 16 | Microstructural influences on the high cycle fatigue life dispersion and damage mechanism in a metastable β titanium alloy显示文摘In this work,the effect of microstructure features on the high-cycle fatigue behavior of Ti-7Mo-3Nb-3Cr-3Al(Ti-7333)alloy is investigated.Fatigue tests were carried out at room temperature in lab air atmosphere using a sinusoidal wave at a frequency of 120 Hz and a stress ratio of 0.1.Results show that the fatigue strength is closely related to the microstructure features,especially theα_(p) percentage.The Ti-7333 alloy with a lowerα_(p) percentage exhibits a higher scatter in fatigue data.The bimodal fatigue behavior and the duality of the S-N curve are reported in the Ti-7333 alloy with relatively lowerα_(p) percentage.Crack initiation region shows the compoundα_(p)/βfacets.Facetedα_(p) particles show crystallographic orientation and morphology dependence characteristics.Crack-initiation was accompanied by faceting process across elongatedα_(p) particles or multiple adjacentα_(p) particles.These particles generally oriented for basal slip result in near basal facets.Fatigue crack can also initiate at elongatedα_(p) particle well oriented for prismatic slip.Theβfacet is in close correspondence to{110}or{112}plane with high Schmid factor.Based on the fracture observation and FIB-CS analysis,three classes of fatigue-critical microstructural configurations are deduced.A phenomenological model for the formation ofα_(p) facet in the bimodal microstructure is proposed.This work provides an insight into the fatigue damage process of theβprecipitate strengthened metastableβtitanium alloys. | Zhihong Wu Hongchao Kou Nana Chen Zhixin Zhang Fengming Qiang Jiangkun Fan Bin Tang Jinshan Li | 2021 | Journal of Materials Science & Technology2021，，11： | 0 |
| 17 | Generation of knockout rabbits using transcription activator-like effector nucleases显示文摘Zinc-finger nucleases and transcription activator-like effector nucleases are novel gene-editing platformscontributing to redefine the boundaries of modern biological research. They are composed of a non-specificcleavage domain and a tailor made DNA-binding module, which enables a broad range of genetic modifications byinducing efficient DNA double-strand breaks at desired loci. Among other remarkable uses, these nucleases havebeen employed to produce gene knockouts in mid-size and large animals, such as rabbits and pigs, respectively.This approach is cost effective, relatively quick, and can produce invaluable models for human disease studies,biotechnology or agricultural purposes. Here we describe a protocol for the efficient generation of knockout rabbitsusing transcription activator-like effector nucleases, and a perspective of the field. | Yu Wang Nana Fan Jun Song Juan Zhong Xiaogang Guo Weihua Tian Quanjun Zhang Fenggong Cui Li Li Philip N Newsome Jon Frampton Miguel A Esteban Liangxue Lai | 2014 | Cell Regeneration2014，3，1： | 0 |
| 18 | Generation of multi-gene knockout rabbits using the Cas9/gRNA system显示文摘The prokaryotic clustered regularly interspaced short palindromic repeat(CRISPR)-associated system(Cas)is a simple,robust and efficient technique for gene targeting in model organisms such as zebrafish,mice and rats.In this report,we applied CRISPR technology to rabbits by microinjection of Cas9 mRNA and guided RNA(gRNA)into the cytoplasm of pronuclear-stage embryos.We achieved biallelic gene knockout(KO)rabbits by injection of 1 gene(IL2rg)or 2 gene(IL2rg and RAG1)Cas9 mRNA and gRNA with an efficiency of 100%.We also tested the efficiency of multiple gene KOs in early rabbit embryos and found that the efficiency of simultaneous gene mutation on target sites is as high as 100%for 3 genes(IL2rg,RAG1 and RAG2)and 33.3%for 5 genes(IL2rg,RAG1,RAG2,TIKI1 and ALB).Our results demonstrate that the Cas9/gRNA system is a highly efficient and fast tool not only for single-gene editing but also for multi-gene editing in rabbits. | Quanmei Yan Quanjun Zhang Huaqiang Yang Qingjian Zou Chengcheng Tang Nana Fan Liangxue Lai | 2014 | Cell Regeneration2014，3，1： | 0 |
| 19 | Scutellarin prevents acute alcohol-induced liver injury via inhibiting oxidative stress by regulating the Nrf2/HO-1 pathway and inhibiting inflammation by regulating the AKT,p38 MAPK/NF-κB pathways显示文摘Alcoholic liver disease(ALD)is the most frequent liver disease worldwide,resulting in severe harm to personal health and posing a serious burden to public health.Based on the reported antioxidant and anti-inflammatory capacities of scutellarin(SCU),this study investigated its protective role in male BALB/c mice with acute alcoholic liver injury after oral administration(10,25,and 50 mg/kg).The results indicated that SCU could lessen serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)levels and improve the histopathological changes in acute alcoholic liver;it reduced alcohol-induced malondialdehyde(MDA)content and increased glutathione peroxidase(GSH-Px),catalase(CAT),and superoxide dismutase(SOD)activity.Furthermore,SCU decreased tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),and IL-1βmessenger RNA(mRNA)expression levels,weakened inducible nitric oxide synthase(iNOS)activity,and inhibited nucleotide-binding oligomerization domain(NOD)-like receptor protein 3(NLRP3)inflammasome activation.Mechanistically,SCU suppressed cytochrome P450 family 2 subfamily E member 1(CYP2E1)upregulation triggered by alcohol,increased the expression of oxidative stress-related nuclear factor erythroid 2-related factor 2(Nrf2)and heme oxygenase-1(HO-1)pathways,and suppressed the inflammation-related degradation of inhibitor of nuclear factor-κB(NF-κB)-α(IκBα)as well as activation of NF-κB by mediating the protein kinase B(AKT)and p38 mitogen-activated protein kinase(MAPK)pathways.These findings demonstrate that SCU protects against acute alcoholic liver injury via inhibiting oxidative stress by regulating the Nrf2/HO-1 pathway and suppressing inflammation by regulating the AKT,p38 MAPK/NF-κB pathways. | Xiao ZHANG Zhicheng DONG Hui FAN Qiankun YANG Guili YU Enzhuang PAN Nana HE Xueqing LI Panpan ZHAO Mian FU Jingquan DONG | 2023 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2023，24，7： | 0 |
| 20 | Progranulin regulation of autophagy contributes to its chondroprotective effect in osteoarthritis显示文摘Progranulin(PGRN)is a multifunctional growth factor involved in many physiolog-ical processes and disease states.The apparent protective role of PGRN and the importance of chondrocyte autophagic function in the progression of osteoarthritis(OA)led us to investi-gate the role of PGRN in the regulation of chondrocyte autophagy.PGRN knockout chondro-cytes exhibited a deficient autophagic response with limited induction following rapamycin,serum starvation,and IL-1b-induced autophagy.PGRN-mediated anabolism and suppression of IL-1b-induced catabolism were largely abrogated in the presence of the BafA1 autophagy inhibitor.Mechanistically,during the process of OA,PGRN and the ATG5eATG12 conjugate form a protein complex;PGRN regulates autophagy in chondrocytes and OA through,at least partially,the interactions between PGRN and the ATG5eATG12 conjugate.Furthermore,the ATG5eATG12 conjugate is critical for cell proliferation and apoptosis.Knockdown or knockout of ATG5 reduces the expression of ATG5eATG12 conjugate and inhibits the chondroprotective effect of PGRN on anabolism and catabolism.Overexpression of PGRN partially reversed this effect.In brief,the PGRN-mediated regulation of chondrocyte autophagy plays a key role in the chondroprotective role of PGRN in OA.Such studies provide new insights into the pathogen-esis of OA and PGRN-associated autophagy in chondrocyte homeostasis. | Yiming Pan Yuyou Yang Mengtian Fan Cheng Chen Rong Jiang Li Liang Menglin Xian Biao Kuang Nana Geng Naibo Feng Lin Deng Wei Zheng Fengmei Zhang Xiaoli Li Fengjin Guo | 2023 | Genes & Diseases2023，10，4： | 0 |
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