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| 1 | The Reference Genome Sequence of Scutellaria baicalensis Provides Insights into the Evolution of Wogonin Biosynthesis显示文摘Scutellaria baicalensis Georgi is important in Chinese traditional medicine where preparations of dried roots,'Huang Qin,' are used for liver and lung complaints and as complementary cancer treatments. We report a high-quality reference genome sequence for S. baicalensis where 93% of the 408.14-Mb genome has been assembled into nine pseudochromosomes with a super-N50 of 33.2 Mb. Comparison of this sequence with those of closely related species in the order Lamiales, sesamum indicum and Salvia splendens,revealed that a specialized metabolic pathway for the synthesis of 4'-deoxyflavone bioactives evolved in the genus Scu-tellaria. We found that the gene encoding a specific cinnamate coenzyme A ligase likely obtained its newfunc- tion following recent mutations, and that four genes encoding enzymes in the 4'-deoxyflavone pathway are present as tandem repeats in the genome of S. baicalensis. Further analyses revealed that gene duplications, segmental duplication, gene amplification, and point mutations coupled to gene neo- and subfunctionaliza-tions were involved in the evolution of 4'-deoxyflavone synthesis in the genus Scutellaria. Our study not only provides significant insight into the evolution of specific flavone biosynthetic pathways in the mint family, Lamiaceae, but also will facilitate the development of tools for enhancing bioactive productivity by metabolic engineering in microbes or by molecular breeding in plants. The reference genome of S. baicalensis is also useful for improving the genome assemblies for other members of the mint family and offers an important foundation for decoding the synthetic pathways of bioactive compounds in medicinal plants. | Qing Zhao Jun Yang Meng-Ying Cui Jie Liu Yumin Fang Mengxiao Yan Wenqing Qiu Huiwen Shang Zhicheng Xu Reheman Yidiresi Jing-Ke Weng Tomas Pluskal Marielle Vigouroux Burkhard Steuernagel Yukun Wei Lei Yang Yonghong Hu Xiao-Ya Chen Cathie Martin | 2019 | Molecular Plant2019，12，7： | 23 |
| 2 | Transduction of the Hedgehog signal through the dimerization of Fused and the nuclear translocation of Cubitus interruptus显示文摘分泌蛋白质的刺猬(Hh ) 家庭为在脊椎动物和无脊椎动物的开发是必要的。作为主要 morphogens 之一在后生动物的开发期间,分级的 Hh 信号通过它的细胞质的尾巴的微分 phosphorylation 是越过由 Smoothened (Smo ) 的血浆膜的 transduced,导致小径激活和目标基因的微分表达式。然而,怎么 Smo transduces 经由建筑群糟糕仍然是的 Costal2 (Cos2 )/Fused (Fu ) 的分级的 Hh 信号理解。这里我们在场对由翻译 Smo phosphorylation 信息到 Fu dimerization 和肘脉 interruptus (Ci ) 的一个 Hh 坡度的房间反应的一个模型原子本地化信息。我们的调查结果建议 Smo 的 phosphorylated C 终点招募 Cos2/Fu 建筑群到通过在 Smo 和 Cos2 之间的相互作用的膜,它进一步导致 Fu dimerization。使聚合成二聚物的 Fu 是 phosphorylated 和 transduces 由 phosphorylating Cos2 和 Fu (苏(fu )) 的 Suppressor 的 Hh 信号。我们进一步证明这个过程支持全身的 Ci (Ci155 ) 和 Cos2 或苏(fu ) 的分离,并且导致 Ci155 的 translocation 进原子核,激活目标基因的表示。 | Yanyan Zhang Feifei Mao Yi Lu Wenqing Wu Lei Zhang Yun Zhao | 2011 | Cell Research2011，21，10： | 12 |
| 3 | Modulating the threshold voltage of oxide nanowire field-effect transistors by a Ga^+ ion beam显示文摘在这份报纸,我们报导一个方法改变 SnO 的阀值电压<潜水艇class=“ a-plus-plus ”> 2 并且在里面<潜水艇class=“ a-plus-plus ”> 2 O <潜水艇class=“ a-plus-plus ”>由 Ga 的 3 只 nanowire 晶体管<啜class=“ a-plus-plus ”>+离子照耀。不同于在更早的报告的结果, SnO 的阀值电压<潜水艇class=“ a-plus-plus ”> 2 并且在里面<潜水艇class=“ a-plus-plus ”> 2 O <潜水艇class=“ a-plus-plus ”>在在 Ga 以后的否定的门电压方向的 3 nanowire 地效果晶体管(联邦货物税)移动<啜class=“ a-plus-plus “>+离子照耀。更小的阀值电压,由 Ga 完成了 < 啜 class= “ a-plus-plus ” >+ 离子照耀,为高效、低电压的操作被要求。阀值电压移动能被归因于 Ga 引起的表面缺点的降级 < 啜 class= “ a-plus-plus ” >+ 离子照耀。在照耀以后,当前的开/关比率稍微衰退,但是离 10 仍然靠近 < 啜 class= “ a-plus-plus ” > 6 。结果显示那 Ga < 啜 class= “ a-plus-plus ” >+ 离子横梁照耀在改进氧化物 nanowire 联邦货物税的表演起一个重要作用。 | Wenqing Li Lei Liao Xiangheng Xiao Xinyue Zhao Zhigao Dai Shishang Guo Wei Wu Ying Shi Jinxia Xu Feng Ren Changzhong Jiang | 2014 | Nano Research2014，7，11： | 5 |
| 4 | Radiotherapy combined with nimotuzumab for elderly esophageal cancer patients:A phaseⅡclinical trial显示文摘Objective:To investigate the safety and efficacy of nimotuzumab combined with radiotherapy for elderly patients with non-resectable esophageal carcinoma(EC).Methods:Eligible patients were aged 70 years or older and had treatment-naive,histologically proven inoperable locally advanced EC.Enrolled patients received radiotherapy with a total dose of 50-60 Gy in 25-30 fractions,concurrent with weekly infusion of nimotuzumab.The primary end point was the rate of more than grade 3 toxicities.Results:From June 2011 to July 2016,46 patients with stageⅡ-IV EC with a median age of 76.5 years were enrolled.There were 10,28 and 8 patients with stageⅡ,III and IV disease,respectively.The common acute toxicities included esophagitis(grade 1-2,75.4%;grade 3,8.7%),pneumonitis(grade 1,4.3%;grade 2,6.5%;grade3,2.2%),leukopenia(grade 1-2,60.9%;grade 3-4,4.4%),gastrointestinal reaction(grade 1-2,17.3%;grade 3,2.2%),thrombocytopenia(grade 1-2,21.7%;grade 3,2.2%),and radiothermitis(grade 1-2,39.2%).The incidence of grade 3-4 adverse effects was 17.4%.No grade 5 toxicities were observed.Clinical complete response,partial response,stable disease,and progressive disease were observed in 1(2.2%),31(67.4%),12(26.1%),and 2(4.3%)patients,respectively.The median overall survival(OS)and progression-free survival(PFS)were 17 and 10 months,respectively.The 2-,3-,and 5-year OS and PFS rates were 30.4%,21.7%,19.6%,and 26.1%,19.6%,19.6%,respectively.Conclusions:Nimotuzumab combined with radiotherapy is a safe and effective therapy for elderly patients who are not surgical candidates.Further studies are warranted to confirm its therapeutic effects in elderly EC patients. | Xu Yang Yirui Zhai Nan Bi Tao Zhang Lei Deng Wenqing Wang Xin Wang Dongfu Chen Zongmei Zhou Luhua Wang Jun Liang | 2021 | Chinese Journal of Cancer Research2021，33，1： | 4 |
| 5 | High-throughput screening identifies established drugs as SARS-CoV-2 PLpro inhibitors显示文摘A new coronavirus(SARS-CoV-2)has been identified as the etiologic agent for the COVID-19 outbreak.Currently,effective treatment options remain very limited for this disease;therefore,there is an urgent need to identify new anti-COVID-19 agents.In this study,we screened over 6,000 compounds that included approved drugs,drug candidates in clinical trials,and pharmacologically active compounds to identify leads that target the SARS-CoV-2 papain-like protease(PLpro).Together with main protease(Mpro),PLpro is responsible for processing the viral replicase polyprotein into functional units.There-fore,it is an attractive target for antiviral drug develop-ment.Here we discovered four compounds,YM155,cryptotanshinone,tanshinone I and GRL0617 that inhibit SARS-CoV-2 PLpro with IC50 values ranging from 1.39 to 5.63 pmol/L.These compounds also exhibit strong antiviral activities in cell-based assays.YM155,an anti-cancer drug candidate in clinical trials,has the most potent antiviral activity with an EC50 value of 170 nmol/L.In addition,we have determined the crystal structures of this enzyme and its complex with YM155,revealing a unique binding mode.YM155 simultaneously targets three'hot'spots on PLpro,including the substrate-binding pocket,the interferon stimulating gene product 15(ISG15)binding site and zinc finger motif.Our results demonstrate the efficacy of this screening and repur-posing strategy,which has led to the discovery of new drug leads with clinical potential for COVID-19 treatments. | Yao Zhao Xiaoyu Du Yinkai Duan Xiaoyan Pan Yifang Sun Tian You Lin Han Zhenming Jin Weijuan Shang Jing Yu Hangtian Guo Qianying Liu Yan Wu Chao Peng Jun Wang Chenghao Zhu Xiuna Yang Kailin Yang Ying Lei Luke W.Guddat Wenqing Xu Gengfu Xiao Lei Sun Leike Zhang Zihe Rao Haitao Yang | 2021 | Protein & Cell2021，12，11： | 4 |
| 6 | Effect of algal flocculation on dissolved organic matters using cationic starch modified soils显示文摘Modified soils(MSs) are being increasingly used as geo-engineering materials for the sedimentation removal of cyanobacterial blooms. Cationic starch(CS) has been tested as an effective soil modifier, but little is known about its potential impacts on the treated water.This study investigated dissolved organic matters in the bloom water after algal removal using cationic starch modified soils(CS-MSs). Results showed that the dissolved organic carbon(DOC) could be decreased by CS-MS flocculation and the use of higher charge density CS yielded a greater DOC reduction. When CS with the charge density of 0.052, 0.102 and0.293 meq/g were used, DOC was decreased from 3.4 to 3.0, 2.3 and 1.7 mg/L, respectively.The excitation–emission matrix fluorescence spectroscopy and UV254 analysis indicated that CS-MS exhibits an ability to remove some soluble organics, which contributed to the DOC reduction. However, the use of low charge density CS posed a potential risk of DOC increase due to the high CS loading for effective algal removal. When CS with the charge density of 0.044 meq/g was used, DOC was increased from 3.4 to 3.9 mg/L. This study suggested, when CS-MS is used for cyanobacterial bloom removal, the content of dissolved organic matters in the treated water can be controlled by optimizing the charge density of CS. For the settled organic matters, other measures(e.g., capping treatments using oxygen loaded materials) should be jointly applied after algal flocculation. | Wenqing Shi Lei Bi Gang Pan | 2016 | Journal of Environmental Sciences2016，28，7： | 3 |
| 7 | Distinct functions of polycomb group proteins in regulating Ci transcription in developing Drosophila显示文摘 | Xiangdong Lv Hao Chen Shuo Zhang Zhao Zhang Chenyu Pan Yuanxin Xia Jialin Fan Wenqing Wu Yi Lu Lei Zhang Hailong Wu Yun Zhao | 2018 | Journal of Molecular Cell Biology2018，10，5： | 3 |
| 8 | Modes of genetic adaptations underlying functional innovations in the rumen显示文摘The rumen is the hallmark organ of ruminants and hosts a diverse ecosystem of microorganisms that facilitates efficient digestion of plant fibers.We analyzed 897 transcriptomes from three Cetartiodactyla lineages:ruminants,camels and cetaceans,as well as data from ruminant comparative genomics and functional assays to explore the genetic basis of rumen functional innovations.We identified genes with relatively high expression in the rumen,of which many appeared to be recruited from other tissues.These genes show functional enrichment in ketone body metabolism,regulation of microbial community,and epithelium absorption,which are the most prominent biological processes involved in rumen innovations.Several modes of genetic change underlying rumen functional innovations were uncovered,including coding mutations,genes newly evolved,and changes of regulatory elements.We validated that the key ketogenesis rate-limiting gene(HMGCS2)with five ruminant-specific mutations was under positive selection and exhibits higher synthesis activity than those of other mammals.Two newly evolved genes(LYZ1 and DEFB1)are resistant to Gram-positive bacteria and thereby may regulate microbial community equilibrium.Furthermore,we confirmed that the changes of regulatory elements accounted for the majority of rumen gene recruitment.These results greatly improve our understanding of rumen evolution and organ evo-devo in general. | Xiangyu Pan Yudong Cai Zongjun Li Xianqing Chen Rasmus Heller Nini Wang Yu Wang Chen Zhao Yong Wang Han Xu Songhai Li Ming Li Cunyuan Li Shengwei Hu Hui Li Kun Wang Lei Chen Bin Wei Zhuqing Zheng Weiwei Fu Yue Yang Tingting Zhang Zhuoting Hou Yueyang Yan Xiaoyang Lv Wei Sun Xinyu Li Shisheng Huang Lixiang Liu Shengyong Mao Wenqing Liu Jinlian Hua Zhipeng Li Guojie Zhang Yulin Chen Xihong Wang Qiang Qiu Brian PDalrymple Wen Wang Yu Jiang | 2021 | Science China(Life Sciences)2021，64，1： | 3 |
| 9 | Fsh-Pc-Sce complex mediates active transcription of Cubitus interruptus (Ci)显示文摘表明小径的刺猬(Hh ) 在胚胎的开发和成年织物动态平衡起重要作用。如此的生物功能被抄写因素肘脉 interruptus (Ci ) 调停。然而,受动器 Ci 本身的 transcriptional 规定糟糕被调查。通过一幅基于 RNAi 的基因屏幕,我们识别了那女性无菌(1 ) homeotic (Fsh ) ,抄写激活剂,直接激活 Ci 抄写。生物化学试金在 Fsh 之中表明了物理相互作用,性梳子额外(Sce ) ,并且 Polycomb (Pc ) 。功能的试金进一步证明 Pc 和 Sce 为 Ci 被要求表示,没被 Engrailed 的 derepression 多半调停,) , Ci 的一个抑压者,在 Pc 或 Sce 异种房间。最后,我们提供证明 Pc/Sce 在 Ci 地点便于 Fsh 的绑定并且在 Fsh 和 Pc 之间的物理相互作用为调停 Fsh 的 Ci 抄写是必要的证据。一起拿,我们不仅揭开那 Ci 被复杂的 Fsh-Pc-Sce transcriptionally 调整而且在 transcriptional 为在 Fsh 和 PcG 蛋白质之间的协作提供证据规定。 | Xiangdong Lv Hao Chen Shuo Zhang Zhao Zhang Chenyu Pan Yuanxin Xia Jialin Fan Wenqing Wu Yi Lu Lei Zhang Hailong Wu Yun Zhao | 2018 | Journal of Molecular Cell Biology2018，10，5： | 2 |
| 10 | Study on the influencing factors to reduce the recovery time of superconducting tapes and coils for the DC superconducting fault current limiter applications显示文摘Superconducting fault current limiters(SFCLs)are important in improving the stability of electrical power systems.However,the existing resistive-type DC SFCL has a long re-covery time,which cannot meet the requirements of power system auto-reclosure.Thus,it is necessary to investigate the methods of reducing recovery time of SFCLs.In the simulations on superconducting tapes,there is a lack of investigation about the effects of design factors of superconducting tapes and coils such as material,thickness,coil radius,coil winding method on recovery characteristics.The objective of this study is to obtain the effects of material and geometric properties of superconducting tapes and coils on recovery characteristics and obtain methods to reduce the recovery time.The recovery times under different conducting currents are also compared by simulations and exper-iments to verify simulation accuracy.The maximum error is below 40%and most of the cases have an error below 10%.Besides,by increasing tape thickness,the recovery time can be reduced by 22%.Tape materials and coil winding methods can be designed ac-cording to the auto-reclosure and current-limiting requirements of the power systems.The research provided directions for the design of superconducting tape and super-conducting coil for resistive-type DC SFCLs. | Bin Xiang Wenqing Wang Hongxu Li Lei Gao Zhiyuan Liu Yingsan Geng Jianhua Wang Youping Tu | 2022 | High Voltage2022，7，3： | 1 |
| 11 | Crystal Structures of c-Src Reveal Features of Its Autoinhibitory Mechanism显示文摘 | Wenqing Xu Amish Doshi Ming Lei Michael J Eck Stephen C Harrison | 1999 | Molecular Cell1999，，5： | 1 |
| 12 | Ultrahigh Thermoelectric Performance by Electron and Phonon Critical Scattering in Cu2Se1‐xIx显示文摘 | Huili Liu Xun Yuan Ping Lu Xun Shi Fangfang Xu Ying He Yunshan Tang Shengqiang Bai Wenqing Zhang Lidong Chen Yue Lin Lei Shi He Lin Xingyu Gao Xingmin Zhang Hang Chi Ctirad Uher | 2013 | Mater2013，，45： | 1 |
| 13 | Susceptibility behaviour and specific heat anomaly in single crystals of alanine and valine显示文摘 | Wenqing Wang Xiangrong Sheng Haofang Jin Jilan Wu Bo Yin Jingwei Li Zhongxian Zhao Hongshun Yang Fengming Lou Zhizhong Zhuang Guanyuan Yu Lei Shi Zhaojia Chen | 1996 | Journal of Biological Physics1996，，2： | 1 |
| 14 | Epidemiology Features and Effectiveness of Vaccination and Non-Pharmaceutical Interventions of Delta and Lambda SARS-CoV-2 Variants显示文摘As the epidemic time of COVID-19 outbreaks worldwide has extended and the range of prevalence has expanded,SARS-CoV-2 viruses have continuously evolved and mutated,and multiple virus variants have successively emerged.Recently,the Delta and Lambda variants have attracted considerable attention in China for their transmissibility,infective incubation period,and pathogenicity.In this review,we describe the epidemic characteristics and prevention and control measures for Delta and Lambda. | Wenqing Bai Yue Gu Haoliang Liu Lei Zhou | 2021 | China CDC weekly2021，3，46： | 1 |
| 15 | Reduced high‐molecular‐weight adiponectin is an independent risk factor for cardiovascular lesions in hypercholesterolaemic patients显示文摘 | Wenqing Wang Wenjuan Xing Haifeng Zhang Mingge Ding Lei Shang Wayne Bond Lau Xiaoming Wang Rong Li | 2013 | Clin Endocrinol2013，，4： | 1 |
| 16 | Controlled synthesis of Nd(OH) 3 and Nd 2 O 3 nanoparticles by microemulsion method显示文摘 | Wenqing Zhu Jin Ma Lei Xu Wenzhao Zhang Yashao Chen | 2010 | Materials Chemistry and Physics2010，，： | 1 |
| 17 | Air-stable diradical dications with ferromagnetic interaction exceeding the thermal energy at room temperature: from a monomer to a dimer显示文摘Two tetraazacyclophane dications(1^(2+) and 2^(2+)) with different remote substituents have been synthesized, isolated and characterized.Their electronic structures and physical property were studied by various spectroscopic techniques, single crystal X-ray diffraction,super conducting quantum interference device(SQUID) measurements and theoretical calculations. The dications have triplet ground states with ferromagnetic interaction exceeding the thermal energy at room temperature. The solid-state structures of these species were tunable by substituent effect, with 1^(2+) as a monomer and 2^(2+) as a dimer. | Wenqing Wang Lei Wang Sheng Chen Wenbang Yang Zaichao Zhang Xinping Wang | 2018 | Science China Chemistry2018，61，3： | 1 |
| 18 | Heritable expansion of the genetic code in mouse and zebrafish显示文摘 | Yuting Chen Ji Ma Wenqing Lu Meilin Tian Marion Thauvin Chonggang Yuan Michel Volovitch Qian Wang Jeff Holst Mingyao Liu Sophie Vriz Shixin Ye Lei Wang Dali Li | 2017 | Cell Research2017，27，2： | 1 |
| 19 | Boosting tribo-catalytic conversion of H_(2)O and CO_(2)by Co_(3)O_(4)nanoparticles through metallic coatings in reactors显示文摘In recent years,more and more metal oxides have been finding critical tribo-catalytic applications.Presently,we have explored the tribo-catalytic conversion of H_(2)O and CO_(2)using Co_(3)O_(4)nanoparticles and obtained some surprising results.In an as-received 150 mL glass reactor enclosed with 10 mL of H_(2)O,0.10 g of Co_(3)O_(4)nanoparticles,1 atm of CO_(2),and a Teflon magnetic rotary disk,we observed the production of as much as 57.41μmol/L of H_(2),0.15μmol/L of CH_(4),and 0.21μmol/L of CO after 5 h of magnetic stirring.Metallic coatings of Cu,Ni,SUS316,Ti,Nb,Mo,and W were further introduced on reactor bottoms separately.For those coatings of Ni,SUS316,Ti,and Nb,the reduction of CO_(2)was dramatically enhanced,and C_(2+)products of C_(2)H_(6)and C_(2)H_(4)were observed.Especially for the Ti coating,the amounts of H_(2)and CH_(4)were increased by 2 and 26 times from those for the glass bottom,respectively,and the amounts of C_(2)H_(6)and C_(2)H_(4)were very impressive.The Co_(3)O_(4)nanoparticles were proven chemically stable under magnetic stirring in water,and hydroxyl radicals and superoxide radicals have been detected for the Co_(3)O_(4)nanoparticles under magnetic stirring through fluorescence spectroscopy and electron paramagnetic resonance spectroscopy analyses.These findings not only reveal outstanding capability of Co_(3)O_(4)to generate multicarbon products from H_(2)O and CO_(2)through tribo-catalysis but also highlight a promising potential of tribo-catalysis as a whole to harness mechanical energy for addressing energy shortages and environmental pollution. | Xuchao Jia Hongbo Wang Hua Lei Chenyue Mao Xiaodong Cui Yong Liu Yanmin Jia Wenqing Yao Wanping Chen | 2023 | Journal of Advanced Ceramics2023，12，10： | 0 |
| 20 | Input Generation via Taintdata Identification:Finding Hidden Path in the Environment-Intensive Program显示文摘Concolic testing is an integrated approach of symbolic execution and dynamic analysis, which is widely adopted by security researchers for program behavior analysis. This approach fails on hidden path discovery of environment-intensive program. We investigated on existing concolic testing tools and found out that several of them does not take this issue into account while others solved this issue with overloaded working model. We proposed a systematic and unified approach of automatically identifying and modifying the output of the Data input interacting functions(DIIF) based on fine-grained taint analysis, which detects and updates the data interacting with the runtime environment and generating a new customized set of inputs to execute hidden paths, to reveal the hidden paths on only particular runtime configuration or context. A prototype was developed and evaluated with a set of complex and environment-intensive programs. The experimental result demonstrated that our approach could detect the DIIF precisely and improve the code coverage. | LEI Xue HUANG Wei FAN Wenqing YANG Yixian | 2015 | Chinese Journal of Electronics2015，24，3： | 0 |
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