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| 1 | Interlayer interactions in anisotropic atomically thin rhenium diselenide显示文摘在这个工作,我们学习夹层声子颤动模式, layer-numberdependent 原子地薄的金来 diselenide 的光 bandgap,和各向异性的光致发光(PL ) 系列(ReSe 2) 第一次。在 ReSe 2 的 ultralow 频率夹层拉曼系列和解决极化的高频率拉曼系列允许它的层数字和水晶取向的鉴定。而且, PL 大小显示出材料的各向异性的光排放紧张,它的 bandgap 在单层在体积从 1.26 eV 增加到 1.32 eV。拉曼模式和 PL 系列的层数字依赖的学习揭示相对弱的货车 der Waals 相互作用并且二维(2D ) 在原子地薄的 ReSe 2 的量监禁。在单层和多层的 ReSe 2 的吸引人的各向异性的夹层相互作用和悦耳的光转变的试验性的观察在在在红外线附近的光谱工作的各向异性的 optoelectronic 设备的发展为这材料的进一步的探索建立基础,它为在光通讯并且红外线的察觉到的许多应用是重要的。 | Huan Zhao Jiangbin Wu Hongxia Zhong Qiushi Guo Xiaomu Wang Fengnian Xia Li Yang Pingheng Tan Han Wang | 2015 | Nano Research2015，8，11： | 14 |
| 2 | Compressed sensing SAR imaging based on sparse representation in fractional Fourier domain显示文摘Compressed sensing(CS)is a new technique of utilizing a priori knowledge on sparsity of data in a certain domain for minimizing necessary number of measurements.Based on this idea,this paper proposes a novel synthetic aperture radar(SAR)imaging approach by exploiting sparseness of echo data in the fractional Fourier domain.The effectiveness and robustness of the approach are assessed by some numerical experiments under various noisy conditions and different measurement matrices.Experimental results have shown that,the obtained images by using the CS technique depend on measurement matrix and have higher output signal to noise ratio than traditional pulse compression technique.Finally simulated and real data are also processed and the achieved results show that the proposed approach is capable of reconstructing the image of targets and effectively suppressing noise. | BU HongXia BAI Xia TAO Ran | 2012 | Science China(Information Sciences)2012，55，8： | 12 |
| 3 | Apoptosis of Spodoptera litura larval hemocytes induced byheavy metal zinc显示文摘By adding different amount of zinc into the artificial medium of the insect larvae, the zinc-induced apoptosis of the larvae haemocytes of the herbivorous insect Spodoptera litura Fabricius was investigated with flow cy- tometer. The results showed that the increase of zinc dose in the artificial feed led to the accumulations of zinc in the lar- val hemolymph and fat body, and more zinc was accumu- lated in fat body than in hemolymph. The apoptosis of hemocytes was significantly induced at high zinc concentra- tion (1000 mg·kg?1) in the insect diet, and the apoptosis rate was 63.63%, which was remarkably higher than that at con- trol and lower concentrations (50―500 mg·kg?1). This sug- gests that the high dose of zinc in the artificial diet of S. litura larvae could induce the apoptosis of the larval hemocytes of S. litura. | XIA Qiang SUN Hongxia HU Xinjun SHU Yinghua GU Dexiang ZHANG Guren | 2005 | Chinese Science Bulletin2005，50，24： | 9 |
| 4 | First observation of Karlodinium venef icum from the East China Sea and the coastal waters of Germany显示文摘Harmful algal blooms often occurred in the East China Sea (ECS) and the German coastal waters of the North Sea but Karlodinium veneficum had not been taxonomically reported. Two strains of Karlodinium (LAMB090611 and LAMB010601) were isolated from the two areas in 2009. The morphological characteristics and molecular phylogeny of two strains are compared on the basis of observation of a light microscope, a scanning electron microscope (SEM), a laser scanning micro-scope (LSM) and an internal transcribed spacer (ITS) sequence data. The mean cell length of strain LAMB090611 is (14.2 ± 1.8) μm (range 11.1-18.7 μm) and mean width is (10.8 ± 1.5) μm (range 8.2- 14.7 μm). The mean cell length of strain LAMB010601 is (15.1 ± 1.2) μm (range 12.7-17.9 μm) and the mean width is (11.4 ± 1.1) μm (range 9.1-14.7 μm), respectively. The two strains are similar in morphological characteristics, including a straight apical groove, distinct ventral pore, sulcal extension, cingulum displacement, two or four irregular shaped chloroplasts within the cell and almost equal sized epicone and hypocone. The large and round nucleus is located at the center or at the hypocone of the cell. The sequence length of the ECS strain LAMB090611 and the German strain LAMB010601 is 640 and 646 bp, respectively. The GC content is 49%. The nucleotide similarity of the two strains is 98.1%. The sequence divergence is 0.003. Both strains are confirmed as Karlo-dinium veneficum (D. Ballantine) J. Larsen and this is the first taxonomic report from China and Germany coastal waters. The population dynamics of this toxic species in the ECS and German coastal waters needs to be investigated in the near future. | WANG Hongxia LU Douding HUANG Haiyan GOBEL Jeaaette DAI Xinfeng XIA Ping | 2011 | Acta Oceanologica Sinica2011，30，6： | 7 |
| 5 | MicroRNA-195 and microRNA-378 mediate tumor growth suppression by epigenetical regulation in gastric cancer显示文摘 | Hongxia Deng Yanan Guo Haojun Song Bingxiu Xiao Weiliang Sun Zhong Liu Xiuchong Yu Tian Xia Long Cui Junming Guo | 2013 | Gene2013，，： | 5 |
| 6 | Effects of dietary nickel on apoptosis of hemocytes of Spodoptera litura (Fabricius) larvae显示文摘Effects of dietary nickel on apoptosis of hemocytes of the 6th instar larvae of Spodoptera litura Fabricius were evaluated using flow cytometry and Laser Scanning Confocal Microscopy (LSCM) by feeding S. litura larvae with artificial diets amended with different concentrations of nickel (1, 5, 10, 20 and 40 mg/kg) for 3 generations. Results showed that the hemocyte apoptosis rate of the 1st generation larvae increased with the increasing nickel concentrations in diet, and the apoptosis rate in the treatment with 40 mg/kg nickel was significantly higher than that in other treatments. In the 2nd generation, the highest hemocytes apoptosis rate was recorded in the treatment of the larvae exposed to 5 mg/kg nickel. The apoptosis rates in the larvae stressed by higher concentrations of nickel were lower than that in control. The apoptosis rates in the larvae exposed to nickel for 3 generations showed similar concentration-responses with that in the 2nd generation. However, the apoptosis rates in the larvae stressed with higher levels of nickel were all significantly inhibited in comparison with that in control. Therefore, effects of nickel stress on apoptosis of S. litura larvae were related with the nickel concentration in diets and the larval generations that were stressed by nickel. | SUN HongXia Xia Qiang TANG WenCheng ZHOU Qiang ZHANG GuRen DANG Zhi | 2010 | Chinese Science Bulletin2010，55，4： | 4 |
| 7 | Synergistic effects of ginseng stem and leaf-extracted ginsenoside and choline on improving learning and memory in rats Association verification experiment in animals with multiple learning and memory disorders显示文摘BACKGROUND: Ginsenoside extracted from the stem and leaf of ginseng (GSL) and choline have both been shown to improve learning and memory functions; however,further studies are needed to understand the synergistic effects of a combination of both. OBJECTIVE: To verify the combined improved synergistic effects of GSL and choline on learning and memory disorders in rats. DESIGN: Control observation. SETTING: Taishan Medical College. MATERIALS: A total of 150 male Kunming mice weighing (20±2) g and 40 healthy male Wistar rats weighing (220±20) g were provided by the Experimental Animal Department of Jilin University. Animal experimentation received confirmed consent from the local ethic committee. GSL was provided by the Department of Chemistry,Norman Bethune Medical University,and choline was provided by the Third Experiment Factory,Shanghai. METHODS: This study was performed at the Life Science Institute,Taishan Medical College from October 2006 to February 2007. ① Scopolamine-induced learning and memory disorders in rats: Forty rats were randomly divided into control group,model group,combination group (400 mg/kg GSL + 200 mg/kg choline),GSL (400 mg/kg) group,and choline (200 mg/kg) group,8 rats/group. Rats were perfused and administrated in the morning,once a day for 14 successive days. Rats in the control group and model group were perfused with 20 mL/kg distilled water and underwent Morris water maze spatial resolution test 1 hour after perfusion on the 10th,11th,and 12th days after administration. Rats also underwent passive step-down avoidance test 1 hour after reperfusion on the 13th and 14th days after administration. Thirty minutes prior to experimentation,rats in the remaining three groups were intraperitoneally (i.p) injected with 2 mg/kg scopolamine,and rats in the control group were i.p. injected with 2 mL/kg saline. ② Scopolamine-induced learning disorder and memory acquired disorder in mice: Fifty mice were randomly divided into control group,model group,combination group (400 mg/kg GSL +200 mg/kg choline),GSL (400 mg/kg) group,and choline (200 mg/kg) group,with 10 mice/group. Mice were perfused and administrated in the morning,once a day for 9 successive days. Mice in the control group and model group were perfused with 20 mL/kg distilled water and underwent passive step down avoidance test 1 hour after reperfusion on the 8th and 9th day after administration. Twenty minutes prior to training,mice in the remaining three groups were i.p. injected with 2 mg/kg scopolamine,and mice in the control group were i.p. injected with 10 mL/kg saline. ③ Sodium nitrite-induced memory consolidation disorder in mice: Grouping,administration,and testing were the same as mentioned above. After training,mice in the remaining three groups were immediately subcutaneously injected with 120 mg/kg sodium nitrite,and mice in the control group were subcutaneously injected with 20 mL/kg saline. ④ Ethanol-induced memory reconsolidation disorder in mice: Grouping,administration,and testing were the same as mentioned above. At 24 hours after training and 20 minutes before retraining,mice in the remaining four groups were perfused with 10 mL/kg ethanol (0.3 volume fraction),and mice in the control group were perfused with 10 mL/kg saline. MAIN OUTCOME MEASURES: Synergistic effects of GSL and choline on learning and memory deficits induced by scopolamine,sodium nitrite,and ethanol in experimental animals. RESULTS: All 40 rats and 150 mice were included in the final analysis. ① Synergistic effects of GSL and choline on learning and memory disorders induced by scopolamine in rats: During passive step-down avoidance and Morris water maze spatial resolution tests,the number of error responses and length of maze training in the model group were significantly greater than in the control group (P < 0.01); while the number of error responses and length of maze training in the combination group were significantly less than in the model group,GSL group,and choline group (P < 0.05-0.01). The Q value was > 1 after combining administration,which suggests that the combination of GSL and choline had synergistic effects. ② Synergistic effects of GSL and choline on learning disorder and memory-acquired disorder induced by scopolamine in mice: During passive step-down avoidance test,the number of error responses in the model group were significantly greater than in the control group (P < 0.01); while the number of error responses in the combination group were significantly less than in the model group,GSL group,and choline group (P < 0.05-0.01). The Q value was > 1 after combining administration,which suggests GSL and choline had synergistic effects. ③ Synergistic effects of GSL and choline on memory sodium nitrate-induced consolidation disorder in mice: During passive step down avoidance test,the number of error responses in the model group were significantly less than in the control group (P < 0.01); while the number of error responses in the combination group were significantly less than in the model group,GSL group,and choline group (P < 0.05-0.01). The Q value was > 1 after combined administration,which suggests GSL and choline had synergistic effects. ④ Synergistic effects of GSL and choline on ethanol-induced memory reconsolidation disorder in mice: During passive step down avoidance test,the number of error responses in the model group were significantly greater than in the control group (P < 0.01); while the number of error responses in the combination group were significantly less than in the model group,GSL group,and choline group (P < 0.05-0.01). The Q value was > 1 after combined administration,which suggests GSL and choline had synergistic effects. CONCLUSION: GSL and choline have synergistic effects on learning and memory functions. | Xiaomin Zhao Hongxia GU Qing Li Xianglin Xie Zuoli Xia Hongxin Cai Ling Zhang Dawei Li Xinnong Wang | 2008 | Neural Regeneration Research2008，3，2： | 3 |
| 8 | Construction of hydrangea-like core-shell SiO_(2)@Ti_(3)C_(2)T_(x)@CoNi microspheres for tunable electromagnetic wave absorbers显示文摘Ti_(3)C_(2)T_(x)MXene shows great potential in the application as microwave absorbers due to its high attenuation ability.However,excessively high permittivity and self-stacking are the main obstacles that constrain its wide range of applications.To tackle these problems,herein,the microspheres of SiO_(2)@Ti_(3)C_(2)T_(x)@CoNi with the hydrangea-like core-shell structure were designed and prepared by a combinatorial electrostatic assembly and hydrothermal reaction method.These microspheres are constructed by an outside layer of CoNi nanosheets and intermediate Ti_(3)C_(2)T_(x)MXene nanosheets wrapping on the core of modified SiO_(2),engendering both homogenous and heterogeneous interfaces.Such trilayer SiO_(2)@Ti_(3)C_(2)T_(x)@CoNi microspheres are“magnetic microsize supercapacitors”that can not only induce dielectric loss and magnetic loss but also provide multilayer interfaces to enhance the interfacial polarization.The optimized impedance matching and core-shell structure could boost the reflection loss(RL)by electromagnetic synergy.The synthesized SiO_(2)@Ti_(3)C_(2)T_(x)@CoNi microspheres demonstrate outstanding microwave absorption(MA)performance benefited from these advantages.The obtained RL value was-63.95 dB at an ultra-thin thickness of 1.2 mm,corresponding to an effective absorption bandwidth(EAB)of 4.56 GHz.This work demonstrates that the trilayer core-shell structure designing strategy is highly efficient for tuning the MA performance of MXene-based microspheres. | Huanhuan Niu Xuewen Jiang Yongde Xia Hailong Wang Rui Zhang Hongxia Li Bingbing Fan Yanchun Zhoua | 2023 | Journal of Advanced Ceramics2023，12，4： | 3 |
| 9 | Association of assisted reproductive technology, germline de novo mutations and congenital heart defects in a prospective birth cohort study显示文摘Emerging evidence suggests that children conceived through assisted reproductive technology(ART)have a higher risk of congenital heart defects(CHDs)even when there is no family history.De novo mutation(DNM)is a well-known cause of sporadic congenital diseases;however,whether ART procedures increase the number of germline DNM(gDNM)has not yet been well studied.Here,we performed whole-genome sequencing of 1137 individuals from 160 families conceived through ART and 205 families conceived spontaneously.Children conceived via ART carried 4.59 more gDNMs than children conceived spontaneously,including 332 paternal and 1.26 maternal DNMs,after correcting for parental age at conception,cigarette smoking,alcohol drinking,and exercise behaviors.Paternal DNMs in offspring conceived via ART are characterized by C>T substitutions at CpG sites,which potentially affect protein-coding genes and are significantly associated with the increased risk of CHD.In addition,the accumulation of non-coding functional mutations was independently associated with CHD and 87.9% of the mutations were originated from the father.Among ART offspring,infertility of the father was associated with elevated paternal DNMs;usage of both recombinant and urinary follicle-stimulating hormone and high-dosage human chorionic gonadotropin trigger was associated with an increase of maternal DNMs.In sum,the increased gDNMs in offspring conceived by ART were primarily originated from fathers,indicating that ART itself may not be a major reason for the accumulation of gDNMs.Our findings emphasize the importance of evaluating the germline status of the fathers in families with the use of ART. | Cheng Wang Hong Lv Xiufeng Ling Hong Li Feiyang Diao Juncheng Dai Jiangbo Du Ting Chen Qi Xi Yang Zhao Kun Zhou Bo Xu Xiumei Han Xiaoyu Liu Meijuan Peng Congcong Chen Shiyao Tao Lei Huang Cong Liu Mingyang Wen Yangqian Jiang Tao Jiang Chuncheng Lu Wei Wu Di Wu Minjian Chen Yuan Lin Xuejiang Guo Ran Huo Jiayin Liu Hongxia Ma Guangfu Jin Yankai Xia Jiahao Sha Hongbing Shen Zhibin Hu | 2021 | Cell Research2021，31，8： | 2 |
| 10 | Synthesis of Ag@Al_2O_3 Core-shell Structure Nanoparticles and Their Enhancement Effect on Dielectric Properties for Ag@Al_2O_3/Polyimide Nanocomposites显示文摘A novel core-shell structure Ag@Al2O3 nano-particles were synthesized and doped into polyimide as conductive fillers to prepare the composite films with high dielectric properties and low dielectric loss. The morphology and structures of the Ag@Al2O3 nano-particles were characterized by transmission electron microscopy(TEM), X-ray diffraction(XRD), and UV-visible spectroscopy. All the results proved that the Ag@Al2O3 nano-particles had a typical core-shell structure, for the Ag particles were coated by Al2O3 shell and the average sizes of Ag@Al2O3 particles were between 30 to 150 nm. The as-prepared Ag@Al2O3 nanoparticles were doped into the polyimide with different mass fractions to fabricate the Ag@Al2O3/PI composite films via in-situ polymerization process. SEM analysis of composite films showed that the Ag@Al2O3 nanoparticles homogeneously dispersed in polyimide matrix with nanoscale. As dielectric materials for electronic packaging systems, the Ag@Al2O3/PI composites exhibited appropriate mechanical properties and enhanced dielectric properties, including greatly enhanced dielectric constant and just a slight increase in dielectric loss. These improvements were attributed to the core-shell structure of fillers and their fine dispersion in the PI matrix. | 翁凌 YAN Liwen LI Hongxia XIA Qianshan LIU Lizhu | 2015 | Journal of Wuhan University of Technology(Materials Science)2015，30，1： | 2 |
| 11 | piR-823, a novel non-coding small RNA, demonstrates in vitro and in vivo tumor suppressive activity in human gastric cancer cells 显示文摘 | Jia Cheng Hongxia Deng Bingxiu Xia etal | 2012 | Cancer Letters2012，815，： | 1 |
| 12 | Effects of ginsenoside of stem and leaf combined with choline on learning and memory ability of rat models with Alzheimer diseases显示文摘BACKGROUND: Central adrenergic nerve and 5-serotonergic nerve can influence central cholinergic nerve on learning and memory and make easy for study; however, ginsenoside of stem and leaf (GSL) can improve functions of central adrenergic nerve; moreover, 5-serotonergic nerve and the combination with choline can produce synergistic effect and enhance learning and memory ability so as to improve learning and memory disorder of patients with Alzheimer disease (AD). OBJECTIVE: To observe the effects of GSL combining with choline on learning and memory of AD model rats.DESIGN: Randomized grouping design and controlled animal study.SETTING: Department of Pharmacology, Taishan Medical College. MATERIALS: The experiment was carried out in the Pharmacological Department of Medical College of Jilin University from October 1996 to January 1997. Forty healthy male Wistar rats of clean grade were randomly divided into 5 groups, including sham-injury group, model group, GSL group, choline group and combination group, with 8 rats in each group. Main medications: GSL with the volume more than 92.8% was provided by Department of Chemistry, Norman Bethune Medical College of Jilin University. Panaxatriol, the main component, was detected with thin layer scanning technique and regarded as the index of GSL quality [(55±1)%, CV = 2%, n = 5]. Choline was provided by the Third Shanghai Laboratory Factory. METHODS: 150 nmol quinolinic acid was used to damage bilateral Meynert basal nuclei of adult rats so as to establish AD models. Rats in GSL, choline and combination groups were intragastric administrated with 400 mg/kg GSL, 200 mg/kg choline (20 mL/kg), and both respectively last for 17 days starting from two days before operation. Rats in sham-injury group and model group were perfused with the same volume of distilled water once in each morning for the same days. ① Passive avoidance step-down test: Five minutes later, rats jumped up safe platform when they were shocked with 36 V alternating current. If rats jumped down from the platform and the feet touched railings, the response was wrong. Numbers of wrong response were recorded within 3 minutes, and then the test was redone after 24 hours. ② Morris water-maze spatial localization task: Swimming from jumping-off to platform directly was regarded as right response. Additionally, 4 successively right responses were regarded as the standard. Each rat was trained 10 times a day with 120 s per time for 3 successive days. The interval was 30 s. Three days later, numbers of right response were recorded. The training times were increased to 30 for unlearned rats. ③ Measurement of activity of choilne acetylase in cerebral cortex: Rats were sacrificed at 17 days after operation to obtain cerebral cortex to measure activity of choilne acetylase with radiochemistry technique. ④ Synergistic effect: It was expressed as Q value: Q value = factual incorporative effect/anticipant incorporative effect; Q ≥ 1 was regarded as synergistic effect. Anticipant incorporative effect = (EA+EB-EA·EB), EA and EB were single timing effect, respectively in GSL group and choline group. E (step-down test and Morris water maze test) = (x in model group - factual value in medicine groups)/x in model group; E (activity of choilne acetylase) = (factual value in medicine groups -x in model group)/x in model group. MAIN OUTCOME MEASURES: ① Passive avoidance step-down test and Morris water-maze spatial localization task in the study of learning and memory; ② activity of choilne acetylase. RESULTS: All 40 rats were involved in the final analysis. ① Passive avoidance response: At learning phase on first day and retesting phase on the next day, numbers of wrong responses within 3 minutes were more in model group than sham operation group, and there was significant difference [(5.88±1.46), (2.25±0.87) times; (2.63±1.06), (0.50±0.53) times; P < 0.01]; numbers of wrong responses within 3 minutes were less in combination group than model group, and there was significant difference [learning phase: (1.12±0.83), (5.88±1.46) times; retesting phase: (0.38±0.74), (2.63±1.06) times, P < 0.01]; moreover, effect was stronger than that in GSL group and choline group. The Q value was 1.07 and 1.59, respectively and it showed synergistic effect. ② Spatial localization task: Training times were more in model group than sham operation group, and there was significant difference [(2.9±2.5), (12.6±3.5) times; P < 0.01]. Training times were less in combination group than model group, and there was significant difference [(11.8±2.4), (27.9±2.5) times, P < 0.01]; moreover, effect was stronger than that in GSL group and choline group. The Q value was 1.07 and it showed synergistic effect. ③ Activity of choilne acetylase: Activity was lower in model group than sham operation group, and there was significant difference [(30.56±8.33), (61.11±8.33) nkat/g; P < 0.01]. Activity was higher in combination group than model group and there was significant difference [(50.00±8.33), (30.56±8.33) nkat/g, P < 0.01]; moreover, effect was stronger than that in GSL group and choline group. The Q value was 1.5 and it showed synergistic effect. CONCLUSION: GSL in combination with choline can synergically improve the disorder of learning and memory of AD model rats. Its mechanism may be involved in enhancing the function of central cholinergic system. | Xiaomin Zhao Xianglin Xie Zuoli Xia Yunsheng Gao Yuyun Zhu Hongxia Gu | 2006 | Neural Regeneration Research2006，1，4： | 1 |
| 13 | A novel SAR imaging algorithm based on compressed sensing显示文摘 | BU Hongxia TAO Ran BAI Xia | 2015 | IEEE Geoscience and Remote Sensing Letters2015，12，5： | 1 |
| 14 | Magnetically recov- erable core-shell nanocomposites γ- Fe2O3 · SiO2 · TiO2-Ag with enhanced photocatalytic activity and anti- bacterial activity 显示文摘 | Bin Cui Hongxia Peng Haiqing Xia | 2013 | Separation and Purification Tech- nology2013，103，： | 1 |
| 15 | Increased dissolution and oral absorption of itraconazole/Soluplus extrudate compared with itraconazole nanosuspension显示文摘 | Keru Zhang Hongxia Yu Qing Luo Shenshen Yang Xia Lin Yu Zhang Bin Tian Xing Tang | 2013 | European Journal of Pharmaceutics and Biopharmaceutics2013，，： | 1 |
| 16 | Allele-specific RNAi selectively silences mutant SODI and achieves significant therapeutic benefit in vivo显示文摘 | ZHOU Hongxia HUANG Yong | 2006 | Neumbiol Dis2006，23，3： | 1 |
| 17 | Activation energy for dibenzofuran desorption from Fe^3+/TiO2 and Ce3+/TiO2 photocatalysts coated onto glass fibres显示文摘 | XIA Qibin LI Zhong XI Hongxia | 2005 | Adsorption Science & Technology2005，23，5： | 1 |
| 18 | Study ofthe catalytic electro-oxidation of ascorbic acid on an electrode modified macrocycliccompounds of Fe(III), Mn(III), Ni(II), and Co(II) with TBP显示文摘 | Jianguo Ren Hongxia Zhang Qiulei Ren Changkun Xia Jie Wan Zibin Qin | 2001 | Journal of ElectroanalyticalChemistry2001，504，： | 1 |
| 19 | Pol II-expressed shRNA knocks down Sod2 gene expression andcauses phenotypes of the gene knockout in mice显示文摘 | Xia Xungang Zhou Hongxia Samper E | 2006 | PLoS Genetics2006，2，1： | 1 |
| 20 | Effects of acupoint versus non-acupoint electroacupuncture on cerebral cortical neuronal Bcl-2, Bax and caspase-3 expression in a rat model of focal cerebral ischemia显示文摘BACKGROUND: Several studies have demonstrated that electroacupuncture by acupoint selection can inhibit cerebral cortical neuronal apoptosis following cerebral ischemia/reperfusion. OBJECTIVE: To validate the effects of electroacupuncture by acupoint selection on the expression level of cortical neuronal anti-apoptotic Bcl-2 protein and the apoptotic executive protein, caspase-3, in rat models of focal cerebral ischemia/reperfusion. DESIGN, TIME AND SETTING: This randomized grouping, neural cell and molecular biology animal experiment was performed at the Laboratory of Pharmacology of Traditional Chinese Medicine and the Laboratory Animal Center of Henan Institute of Traditional Chinese Medicine between November 2006 and May 2007. MATERIALS: Atotal of 40 healthy male adult Sprague-Dawley rats were randomly and evenly divided into four groups: sham-operated, model, electroacupuncture and non-acupoint control. G6895 electro-acupuncture instruments were purchased from Shanghai Huayi Instrument Factory, China. Caspase-3, Bcl-2 and Bax kits were provided by Wuhan Boster Bioengineering Co., Ltd., China. METHODS: Middle cerebral artery occlusion was induced in the model, electroacupuncture and non-acupoint groups. In the electroacupuncture group, the acupoints Jianyu (LI15), Waiguan (SJ5), Biguan (ST31), and Zusanli (ST36) were given electroacupuncture. In the non-acupoint control group, at each time point (immediately after ischemia and after reperfusion, or 2 hours after reperfusion), electroacupuncture was performed at the midpoints of Tianquan (PC2)-Quze (PC 3) line, Quze (PC 3)-Ximen (PC4) line, Zuwuli (LR10)-Yinbao (LR9) line, and Xiguan (LR7)-Zhongdu (LR6) line. Electroacupuncture parameters were set with a continuous wave with a frequency of 10 Hz, wave width 0.6 ms, voltage 1.5–3.0 V, and a duration of 10 minutes. The sham-operated and model groups received only animal fixation without electroacupuncture procedure. MAIN OUTCOME MEASURES: Five rats were selected from each group for specimen preparation. A brain tissue block comprising the frontal lobe and the occipital lobe was cut into five coronal sections of equal-thickness. Neuronal apoptosis was detected by the terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling technique. Expression levels of caspase-3, Bcl-2 and Bax were evaluated by immunohistochemistry. RESULTS: Compared with the sham-operated group, the model group exhibited significantly decreased Bcl-2 expression (P < 0.01), and significantly increased Bax protein expression, number of apoptotic cells, and caspase-3 expression (P < 0.01). In the electroacupuncture group, Bcl-2 protein expression was significantly increased (P < 0.01) compared with the model group, and the Bax protein expression, number of apoptotic cells, and caspase-3 expression were significantly decreased (P < 0.01); the above-mentioned changes were not significant in the non-acupoint group compared with the model group ( P > 0.05). CONCLUSION: Electroacupuncture by acpoint selection can up-regulate Bcl-2 expression and concomitantly inhibit caspase-3 and Bax expression, inhibiting neuronal poptosis in rat cerebral cortex following cerebral ischemia/reperfusion. | Jun Wang Junming Fan Yongshu Dong Xia Huang Hongxia Zhang | 2008 | Neural Regeneration Research2008，3，12： | 1 |
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