|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Genetic diversity analysis of spawner and recaptured populations of Chinese shrimp(Fenneropenaeus chinensis)during stock enhancement in the Bohai Bay based on an SSR marker显示文摘Eight microsatellite markers were used to analyze genetic diversity, level of inbreeding, and effective population size of spawner and recaptured populations of Chinese shrimp(Fenneropenaeus chinensis) during stock enhancement in the Bohai Bay in 2013. A total of 254 and 238 alleles were identified in the spawner and recaptured populations, respectively, and the numbers of alleles(N_a) were 8–63 and 6–60, respectively. The numbers of effective alleles(N_e) were 2.52–21.60 and 2.67–20.72, respectively. The polymorphism information content ranged from 0.529 to 0.952. The observed heterozygosity(H_o) values(0.638–0.910 and 0.712–0.927) were lower than the expected heterozygosity(H_e) values(0.603–0.954 and 0.625–0.952), which indicated that the two populations possessed a rich genetic diversity. In 16 tests(2 populations×8 loci), 13 tests deviated from the HardyWeinberg equilibrium. F_(is) values were positive at seven loci and the inbreeding coefficients(F) of the two populations estimated by trio ML were 13.234% and 11.603%, suggesting that there was a relatively high degree of inbreeding. A certain level of inbreeding depression had occurred in the Chinese shrimp population. F_(st) values ranged from 0 to 0.059, with a mean of 0.028, displaying a low level of genetic differentiation in the two populations. Effective population sizes(3 060.2 and 3 842.8) were higher than the minimum number suggested for retaining the evolutionary potential to adapt to new environmental conditions. For enhancement activity in 2014,the ideal number of captured shrimp spawners should have ranged from 7 686 to 19 214 to maintain genetic diversity and effective population size. Further strategies to adjust the balance of economic cost, fishing effort and ideal number of shrimp spawners to maintain a satisfactory effective population size for ensuring the sustainability of Chinese shrimp are proposed. | WANG Mosang WANG Weiji XIAO Guangxia LIU Kefeng HU Yulong TIAN Tao KONG Jie JIN Xianshi | 2016 | Acta Oceanologica Sinica2016，35，8： | 11 |
| 2 | Using SSR Marker to Trace Chinese Shrimp Fenneropenaeus chinensis Released in Natural Sea-A Feasible Strategy for Assessment of Release Effect in Natural Resources Recovery Program显示文摘Enhancement release has been proven effective in natural resources recovery of Chinese shrimp Fenneropenaeus chinensis in the last several decades in China, however, to assess the effectiveness of enhancement release, we still need to develop a high-efficient and easy-operational method to replace those physical-tagging release method with labor intensive, size-and number-limited. In the present study, single(with maternal known) parentage identification using eight simple sequence repeat(SSR) markers genotype fingerprint was used to trace Chinese shrimp released in Bohai Bay in 2013. A total of 884 shrimp spawners were collected from two hatcheries in Tianjin City respectively after enhancement release of shrimp larvae in May 2013. A total of 844 shrimp samples were recaptured around the release location approximately 4 months after the shrimp larvae were released into the natural sea. Genotype data of 8 SSR loci of the 1,726 samples were used for maternal-offspring parentage identification using CEervus 3.0 software. The allele number in each locus ranged from 8 to 68 with an average value of 33.25, which produced the cumulative exclusion probability with one parent known of all these sight loci up to 99%. Among the 844 recaptured shrimp samples, 448(♂:♀=212:232, gender information was lost for 4 samples) were successfully traced to their 337 maternal parents using a logarithm of odds(LOD) > 3.0 threshold. Among these 337 maternal parents, 253 had a single offspring, 62 had two offspring, 18 had three offspring, 3 had four offspring, and 1 had five offspring. For the first time, a large number of released shrimp were identified from recapture samples, and this study showed that it is possible to trace all released Chinese shrimp without using any type of physical tag in enhancement release activities. This not only means more precise recapture ratio assessment than ever expected, but also this method demonstrates an effective method for large-scale hatchery release as well as for organisms used in hatchery enhancement which are not suitable for physical tagging. | WANG Weiji WANG Mosang XIAO Guangxia HU Yulong SONG Wenping KONG Jie JIN Xianshi | 2018 | 渔业科学进展2018，39，1： | 3 |
| 3 | Role of intestinal bifidobacteria in the pathogenesis of gut-derived bacteria/endotoxin translocation and the effect of bifidobacterial supplement on gut barrier function following burns显示文摘Objective:Early multiple organ dysfunction syndrome appears to be facilitated with bacterial transloca-tion in severely burn injury,yet the mechanisms of bacterial translocation remains in dispute.The aim of this studywas to investigate the potential role of intestinal bifidobacteria in the pathogenesis of gut-derived bacteria/endotoxintranslocation following burns and the effects of bifidohacterial supplement on gut barrier.Methods:Wistar rats wererandomly divided into burn group(Burn,n=60),sham burn g... | Wang Zhongtang Yao Yongming Xiao Guangxia~1 Sheng Zhiyong.Research Department of Burns Institute,304th Hospital,Beijing 100037 | 2003 | 感染.炎症.修复2003，4，3： | 0 |
| 4 | Effect of Different Diet Combinations on WSSV Load and Survival Rate of Fenneropenaeus chinensis Larvae显示文摘Using microalgae( SA),artificial feed( AF),Brachionus plicatilis( BP) and brine shrimp larvae( BS),four diets including SA + AF,SA + AF + BP,SA + AF + BS and SA + AF + BP + BS were formulated and used to rear Fenneropenaeus chinensis larvae. The fertilized eggs fed with SA + AF were divided into disinfection group with iodophor and untreated group,and the fertilized eggs fed with other diets were all disinfected with iodophor. The survival rates,body weight and WSSV load of post larvae at each stage were compared. The results showed that there was no significant difference in the survival rate of prawns at the N-Z stage by feeding with different diets( P > 0. 05),but the survival rate of prawns fed with BP was higher than others( P < 0. 01) at the Z-P stage. The body weight of post larvae( P10) fed with SA + AF + BP was less than that fed with SA + AF + BP + BS( P <0. 01). The WSSV load of prawns( 10. 52 ±3. 3 copies/ng DNA) fed with SA + AF were significantly lower than that of other treatments( P < 0. 05) at the P10 stage. During the P11- P60 stage,four kinds of diets,including Ruditapes philippinarum foot muscle( CF) + artificial feed( AF),R. philippinarum foot muscle radiated by60 Co γ-ray( RCF) + artificial feed( AF),potassium permanganate-disinfected R. philippinarum foot muscle( DCF) + artificial feed( AF) and artificial feed( AF),were used to feed prawns. The prawns fed with CF + AF gained the greatest increase of body weight and body length,but had no significant difference with that fed with AF( P > 0. 05); prawns fed with CF + AF and AF had significant difference with the other two groups( P < 0. 05). The survival rates of prawns fed with four different diets had no significant difference with each other( P > 0. 05). WSSV artificial infection test showed that the accumulated mortality of prawns fed with four diets were higher than 90%,and the difference was not significant( P > 0. 05). | Xiao Guangxia Meng Xianhong Kong Jie Cao Baoxiang Liu Ning Zhang Qingwen Luo Kun Li Zhanjun | 2015 | Animal Husbandry and Feed Science2015，7，6： | 0 |
| 5 | Initiation of two ovarian cell lines from Fugu rubripes(Temminck et.Schlegel)显示文摘The ovary is an excellent system for studying stem cell renewal and differentiation,which is under the control of ovarian somatic cells.In order to understand oogenesis in Fugu rubripes(Temminck et.Schlegel) as a marine fish model of aquaculture importance,we established cell lines called TSOC1 and TSOC2 from a juvenile ovary of this organism.TSOC1 is composed of spindle epithelial-like cells,while the other is cobblestone-like cells.Therefore,TSOC1 and TSOC2 appear to consist of ovarian somatic cells.Growth requirement condition was investigated including temperature,concentration of FBS and p H.Significant fluorescent signals were observed after TSOC1 and TSOC2 cells were transfected with p EGFP-N3 vector,indicating its potential utility for genetic manipulation such as gene function studies.It is shown that these cell lines are effective for infection by the turbot reddish body iridovirus and flounder lymphosystis disease virus as evidenced by the appearance of cytopathic effect and virus propagation in the virus-infected cells,and most convincingly,the observation of viral particles by electron microscopy,demonstrating that TSOC1 and TSOC2 are suitable to study interactions between virus and host cells.It is believed that TSOC1 and TSOC2 will be useful tools to study sex-related events and interactions between primordial germ cells and oogonia cells during oogenesis.Therefore,establishment of ovary cell lines from Fugu rubripes seems to be significant for those research areas. | ZHENG Debin ZHANG Bo SONG Wenping PAN Luqing MA Chao XIAO Guangxia | 2015 | Acta Oceanologica Sinica2015，34，10： | 0 |
| 6 | Cloning,characterization,and expression of a novel member of proteasomal subunits gene in turbot,Scophthalmus maximus显示文摘The proteasome is a large, polymeric protease complex responsible for the degradation of intracellular proteins and generation of peptides that bind to class I major histocompatibility complex(MHC) molecules. This study identified a new member of proteasomal subunits in turbots(Scophthalmus maximus). The fulllength c DNA sequence of turbot proteasomal subunit was obtained. Sequence analysis indicated that its primary structure is highly similar to that of LMP7 from other vertebrates. The relationship between the turbot LMP7 expression and immune responses to pathogen infection was reported. Quantitative reverse transcriptase polymerase chain reaction showed that LMP7 was expressed differently in various tissues, with higher expression in the spleen, liver, muscle, and skin. The LMP7 expression was the highest at 96 h after challenge with lymphocyctis disease virus(LCDV) and at 12 h after challenge with Vibrio anguillarum in the turbot liver, kidney, and spleen. Furthermore, the LMP7 expression distinctly increased in turbot kidney cells at 24 h after challenge with V. anguillarum and at 96 h after challenge with LCDV. These results indicate that the turbot LMP7 protein participates in immune responses and may play a significant role in the immune process. | ZHANG Bo WANG Xianli SONG Wenping ZHENG Debin MA Chao XIAO Guangxia | 2015 | Acta Oceanologica Sinica2015，34，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员