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| 1 | Ubiquitylation of p62/sequestosome1 activates its autophagy receptor function and controls selective autophagy upon ubiquitin stress显示文摘在细胞的 ubiquitin (Ub ) 的改变动态平衡,作为 Ub 知道强调,展示并且影响处于多重条件的细胞的回答,然而,内在的机制不完全地被理解。这里,我们报导 autophagy 受体 p62/sequestosome-1 与结合酶, UBE2D2 和 UBE2D3 的 E2 Ub 交往。内长的 p62 在 Ub 动态平衡的 upregulation 期间经历 E2 依赖的 ubiquitylation,一个条件作为 Ub + 应力称为,那对由 bortezomib 的 Ub overexpression,热吃惊或延长 proteasomal 抑制内在,化学疗法的药。p62 的 Ubiquitylation 破坏 p62 的 UBA 领域的 dimerization,解放它的能力认出为选择 autophagy 的 polyubiquitylated 货物。我们进一步证明这机制可能为在 Ub + 压力条件之上的 autophagy 激活是批评的。机制的描述和在察觉到 Ub 应力并且控制选择 autophagy 的 p62 的规章的角色能帮助理解并且调制细胞的回答到许多内长、环境的挑战,潜在地对 autophagy 相关的病为治疗学的策略的发展打开一条新大街。 | Hong Peng Jiao Yang Guangyi Li Qing You Wen Han Tianrang Li Darning Gao Xiaoduo Xie Byung-Hoon Lee Juan Du Jian Hou Tao Zhang Hai Rao Ying Huang Qinrun Li Rong Zeng Lijian Hui Hongyan Wang Qin Xia Xuemin Zhang Yongning He Masaaki Komatsu Ivan Dikic Daniel Finley Ronggui Hu | 2017 | Cell Research2017，27，5： | 9 |
| 2 | THF_1 mutations lead to increased basal and wound-induced levels of oxylipins that stimulate anthocyanin biosynthesis via COI_1 signaling in Arabidopsis显示文摘Mutants defective in chloroplast development or photosynthesis are liable to accumulate higher levels of anthocyanin in photo-oxidative stress.However,regulatory mechanisms of anthocyanin biosynthesis in the mutants remain unclear.Here,we investigated the mechanism by which the deletion of thylakoid formation1(THF1) leads to an increased level of anthocyanin in Arabidopsis thaliana L.Physiological and genetic evidence showed that the increased level of anthocyanin in thf1 is dependent on coronatine-insensitive1(COM)signaling.Our data showed that thf1 had higher levels of basalα-linolenic acid(α-LeA),and methyl jasmonate(JA)-induced α-LeA and 12-oxophytodienoic acid(OPDA) than the wild type(WT).Consistently,expression levels of phospholipase genes including pPLAIIα and PLA-Iγ1 were elevated in thf1.Furthermore,inhibition of lipase activity by bromoenol lactone,a specific inhibitor of plant pPLA,led to producing identical levels of anthocyanins in WT and thf1 plants.Interestingly,OPDA biosynthesis was triggered by light illumination in isolated chloroplasts,indicating that new protein import into chloroplasts is not required for OPDA biosynthesis.Thus,we conclude that the elevated anthocyanin accumulation in thf1 is attributed to an increase in JA levels.This JA-mediated signaling to coordinate plant metabolism and growth in stress may be conserved in other photosensitive mutants. | Yi Gan Hong Li Ye Xie Wenjuan Wu Maoyin Li Xuemin Wang Jirong Huang | 2014 | Journal of Integrative Plant Biology2014，56，9： | 8 |
| 3 | Genome-and transcriptome-wide association studies provide insights into the genetic basis of natural variation of seed oil content in Brassica napus显示文摘Seed oil content(SOC)is a highly important and complex trait in oil crops.Here,we decipher the genetic basis of natural variation in SOC of Brassica napus by genome-and transcriptome-wide association studies using 505 inbred lines.We mapped reliable quantitative trait loci(QTLs)that control SOC in eight environments,evaluated the effect of each QTL on SOC,and analyzed selection in QTL regions during breeding.Six-hundred and ninety-two genes and four gene modules significantly associated with SOC were identified by analyzing population transcriptomes from seeds.A gene prioritization framework,POCKET(prioritizing the candidate genes by incorporating information on knowledge-based gene sets,effects of variants,genome-wide association studies,and transcriptome-wide association studies),was implemented to determine the causal genes in the QTL regions based on multi-omic datasets.A pair of homologous genes,BnPMT6s,in two QTLs were identified and experimentally demonstrated to negatively regulate SOC.This study provides rich genetic resources for improving SOC and valuable insights toward understanding the complex machinery that directs oil accumulation in the seeds of B.napus and other oil crops. | Shan Tang Hu Zhao Shaoping Lu Liangqian Yu Guofang Zhang Yuting Zhang Qing-Yong Yang Yongming Zhou Xuemin Wang Wei Ma Weibo Xie Liang Guo | 2021 | Molecular Plant2021，14，3： | 6 |
| 4 | Activation of STAT3 stimulates AHSP expression in K562 cells显示文摘Studies on the chaperone proteinα-hemoglobin stabilizing protein(AHSP)reveal that abundant AHSP in erythroid cells enhance the cells’tolerance to oxidative stress imposed by excessα-hemoglobin in pathological conditions.However,the potential intracellular modulation of AHSP expression itself in response to oxidative stress is still unknown.The present study examined the effect and molecular mechanism of STAT3,an oxidative regulator,on the expression of AHSP.AHSP expression increased in K562 cells upon cytokine IL-6-induced STAT3 activation and decreased in STAT3 knock-down K562 cells.Regulation of AHSP in oxidative circumstance was then examined inα-globin-overloaded K562 cells,and real-time PCR showed strengthened expression of both AHSP and STAT3.ChIP analysis showed binding of STAT3 to AHSP promoter and binding was significantly augmented with IL6 stimulation and uponα-globin overexpression.Dual luciferase reporter assays of the wildtype and mutated SB3 element,an IL-6RE site,in the AHSP promoter in K562 cells highlighted the direct regulatory effect of STAT3 on AHSP gene.Finally,direct binding of STAT3 to SB3 site of AHSP promoter was confirmed with EMSA assays.Our work reveals an adaptive AHSP regulation mediated by the redox-sensitive STAT3 signaling pathway,and provides clues to the therapeutic strategy for AHSP enhancement. | CAO Cong ZHAO GuoWei YU Wei XIE XueMin WANG WenTian YANG RuiFeng LV Xiang LIU DePei | 2014 | Science China(Life Sciences)2014，57，5： | 5 |
| 5 | Induced regulatory T cells suppress Tel cells through TGF-β signaling to ameliorate STZ-induced type 1 diabetes mellitus显示文摘Type 1 diabetes mellitus(T1D)is a chronic autoimmune condition in which the immune system destroys insulin-producing pancreatic β cells.In addition to well-established pathogenic effector T cells,regulatory T cells(Tregs)have also been shown to be defective in T1D.Thus,an increasing number of therapeutic approaches are being developed to target Tregs.However,the role and mechanisms of TGF-β-induced Tregs(iTregs)in T1D remain poorly understood.Here,using a streptozotocin(STZ)-induced preclinical T1D mouse model,we found that iTregs could ameliorate the development of T1D and preserve β cell function.The preventive effect was associated with the inhibition of type 1 cytotoxic T(Tel)cell function and rebalancing the Treg/Tc1 cell ratio in recipients.Furthermore,we showed that the underlying mechanisms were due to the TGF-β-mediated combinatorial actions of mTOR and TCF1.In addition to the preventive role,the therapeutic effects of iTregs on the established STZ-T1D and nonobese diabetic(NOD)mouse models were tested,which revealed improved β cell function.Our findings therefore provide key new insights into the basic mechanisms involved in the therapeutic role of iTregs in T1D. | Li Zhou Xuemin He Peihong Cai Ting Li Rongdong Peng Junlong Dang Yue Li Haicheng Li Feng Huang Guojun Shi Chichu Xie Yan Lu Yanming Chen | 2021 | Cellular & Molecular Immunology2021，18，3： | 3 |
| 6 | FORMATION MECHANISMS AND CONTROL STRATEGIES FOR DIOXINS IN INCINERATION PROCESS OF MUNICIPAL SOLID WASTES显示文摘Dioxins, which are of the most toxic materials on the earth, are principal emitted from waste incineration process. The molecular structures, toxicity parameters, such as toxicity equivalency factor, tolerable daily intake and physic-chemical properties of dioxins are briefly summarized. Three formation mechanisms of dioxins in waste incineration process, namely as de novo synthesis, mechanisms involving small organic molecular as precursors and homogenous gas phase reaction mechanism are alto reviewed. The influencing factors for dioxins formation during waste incineration process are also discussed. Three major methods for reducing dioxins emission from waste incineration process are discussed based upon the formation mechanisms and influencing factors. A new waste incineration process with low dioxins emission and low hydrogen chloride corrosion has been proposed based on multi- stage unit operation principal according to formation mechanisms of dioxins and potential production location in waste incinerators. | Yang Xuemin, Li Ji, Lin Weigang, Yao Jianzhong, Wang Xiaoquan and Xie Yusheng (Institute of Chemical Metallurgy, Chinese Academy of Sciences, Beijing 100080) | 2000 | 化工学报2000，51，S1： | 2 |
| 7 | Rapidly developable therapeutic-grade equine immunoglobulin against the SARS-CoV-2 infection in rhesus macaques显示文摘Dear Editor,The unprecedented COVID-19 pandemic caused by SARS-CoV-2 remains ongoing,but there is a lack of fully effective treatments.Convalescent plasma-derived hyperimmune globulins have been a safe and effective treatment but restricted by the difficulties in obtaining sufficient plasma with high antibody titers from a large number of recovered patients.Heterologous antibodies,particularly equine antibodies,have been widely used for decades as the therapeutics against some viral infections or as antivenoms.1 Equine antibodies could be rapidly developed and manufactured into therapeutic antibodies in large quantities under WHO standardized guidelines.Here we explored the development of equine antibody-derived F(ab′)2 as an option to treat COVID-19 by targeting the receptor-binding domain(RBD)of the viral spike protein that is essential for the viral entry into the host cells.2 We observed excellent neutralization titers of the F(ab′)2 in vitro and high potency against SARS-CoV-2 infection in the nonhuman primate rhesus macaques. | Xiaolei Liu Yi Liu Xuemin Jin Zhanlong He Zhen Huang Shumin Sun Yuwei Gao Jingyu Li Qin Ning Zhongping Xie Ningyi Jin Mingyuan Liu | 2022 | Signal Transduction and Targeted Therapy2022，7，8： | 2 |
| 8 | Preparation and evaluation of molecularly imprinted solid-phase microextraction fibers for selective extraction of bisphenol A in complex samples显示文摘 | Feng Tan Hongxia Zhao Xiaona Li Xie Quan Jingwen Chen Xuemin Xiang Xin Zhang | 2009 | Journal of Chromatography A2009，，30： | 1 |
| 9 | QoS provisioning for heterogeneous services in cooperative cognitive radio networks显示文摘 | Alshamrani A Shen Xuemin Xie Liangliang | 2011 | IEEE Journal on Selected Areas in Communications2011，29，4： | 1 |
| 10 | Fluorescent digital image correlation techniques in experimental mechanics显示文摘White light has often been used for surface illumination to acquire images for digital image correlation(DIC) analysis. In recent years, fluorescent imaging technique has been introduced for illumination, surface deformation and topography measurements with applications on various materials including biomaterials(biofilms, etc.) at the microscale. Traditional imaging, with the use of white light, encounters technical issues such as specular reflection owing to moisture or smooth shiny surfaces(e.g., metallic or glass surfaces). As an alternative to white light, fluorescent imaging serves as a solution to resolve the issues of specular reflection.Fluorescent DIC techniques, especially the fluorescent stereo DIC, allow 3 D surface profilometry and deformation measurements at the microscale and submicron scale. Fluorescent stereo imaging under a microscope utilizes emission wavelengths that are different from illumination wavelengths to ensure clear images on any surface that might give reflections at certain angles when white light is used, allowing accurate metrology and deformation measurement. In addition microscopic fluorescent imaging provides nanoscale resolutions surpassing Abbe's diffraction limit. This paper provides a review of the recent advances in fluorescent DIC. | HU ZhenXing XU TingGe WANG XueMin XIE ZhiMing LUO HuiYang HE Yong GUO Lei LI YuanPing GAN RongZhu LU HongBing | 2018 | Science China(Technological Sciences)2018，61，1： | 1 |
| 11 | Preparation and evaluation of molecularly imprinted solid-phase microextraction fibers for selective extraction of bisphenol A in complex samples显示文摘 | Feng Tan Hongxia Zhao Xiaona Li Xie Quan Jingwen Chen Xuemin Xiang Xin Zhang | 2009 | Journal of Chromatography A2009，，30： | 1 |
| 12 | Non-integrating lentiviral vectors based on the minimal S/MAR sequence retain transgene expression in dividing cells显示文摘Safe and efficient gene transfer systems are the basis of gene therapy applications. Non-integrating lentiviral(NIL) vectors are among the most promising candidates for gene transfer tools, because they exhibit high transfer efficiency in both dividing and non-dividing cells and do not present a risk of insertional mutagenesis. However, non-integrating lentiviral vectors cannot introduce stable exogenous gene expression to dividing cells, thereby limiting their application. Here, we report the design of a non-integrating lentiviral vector that contains the minimal scaffold/matrix attachment region(S/MAR) sequence(SNIL), and this SNIL vector is able to retain episomal transgene expression in dividing cells. Using SNIL vectors, we detected the expression of the eGFP gene for 61 days in SNIL-transduced stable CHO cells, either with selection or not. In the NIL group without the S/MAR sequence, however, the transduced cells died under selection for the transient expression of NIL vectors. Furthermore,Southern blot assays demonstrated that the SNIL vectors were retained extrachromosomally in the CHO cells. In conclusion,the minimal S/MAR sequence retained the non-integrating lentiviral vectors in dividing cells, which indicates that SNIL vectors have the potential for use as a gene transfer tool. | Zhen Xu Feng Chen Lingling Zhang Jing Lu Peng Xu Guang Liu Xuemin Xie Wenli Mu Yajun Wang Depei Liu | 2016 | Science China(Life Sciences)2016，59，10： | 0 |
| 13 | Enhanced activation of peroxymonosulfate by CNT-TiO2 under UV-light assistance for efficient degradation of organic pollutants显示文摘In this work,a UV-light assisted peroxymonosulfate(PMS)activation system was constructed with the composite catalyst of multi-walled carbon nanotubes(CNT)-titanium dioxide(TiO2).Under the UV light irradiation,the photoinduced electrons generated from TiO2 could be continuously transferred to CNT for the activation of PMS to improve the catalytic performance of organic pollutant degradation.Meanwhile,the separation of photoinduced electron-hole pairs could enhance the photocatalysis efficiency.The electron spin resonance spectroscopy(EPR)and quenching experiments confirmed the generation of sulfate radical(SO4 ?),hydroxyl radical( OH)and singlet oxygen(1O2)in the UV/PMS/20%CNT-TiO2 system.Almost 100%phenol degradation was observed within 20 min UV-light irradiation.The kinetic reaction rate constant of the UV/PMS/20%CNT-TiO2 system(0.18 min?1)was 23.7 times higher than that of the PMS/Co3O4 system(0.0076 min?1).This higher catalytic performance was ascribed to the introduction of photoinduced electrons,which could enhance the activation of PMS by the transfer of electrons in the UV/PMS/CNT-TiO2 system. | Xuemin Hao Guanlong Wang Shuo Chen Hongtao Yu Xie Quan | 2019 | Frontiers of Environmental Science & Engineering2019，13，5： | 0 |
| 14 | First Beam Commissioning of SSC-Linac as an Injector of SSC显示文摘The construction of the SSC-Linac has made significant progress in 2019.The first beam commissioning of SSC-Linac as an injector of the Separated Sector Cyclotron(SSC)was performed succssfully with ^(40)Ar^(7+) beam and the particle energy of 5.98 MeV/u was obtained at the exit of SSC on December 17,2019. | Du Heng Yin Xuejun Yang Weiqing Li Zhongshan Kong Qiyu Li Xiaoni Yang Shengli Qian Cheng WangHaoning Cong Yan Jin Peng Zhang Ruifeng Han Xiaodong Fu Xin Wang Kai Jing Long Zhang Yong Li Lili Xie Wenjun Xu Xiaowei Wang Shaoming Chen Wenjun Liu Xiaojun Yue Min An Jingrui Zhang Xiang Yan Huaihai Feng Anhui Zhang Shuai Zhang Yongbo Sun Peng Zhu Tieming Sha Xiaoping Xu Jianye Sun Xiaolong Su Xuemin Li Anping Yan Tailai Meng Jun Zhang Wei Wu Junxia Yao Qinggao Yang Yaqing Xu Zhe Sun Liangting Gao Daqing Yuan Youjin Yang Jiancheng Xia Jiawen | 2019 | IMP & HIRFL Annual Report2019，，1： | 0 |
| 15 | Kinetics of Reduction of Iron Oxide in CaO-SiO_2-Al_2O_3-Fe_tO Slags with Graphite Powder显示文摘The reduction rate of Fe_tO in CaO-SiO_2-Al_2O_3-Fe_tO slags with graphite powder has been determined in an alumina crucible at 1 673-1 823 K.The effects of temperature,slag basicity and Fe_tO content on the reduction rate have also been discussed.Test results show that the reduction rate increases with the increase of temperature or Fe_tO concentration in slags,and the reduction rate has a parabolic relation with slag simple basicity or optical basicity.The maximum reduction rate is observed around CaO/SiO_2 = l.5 of molten slags.The reaction order is 1.26 or 1.31,and the reduction activation energy is 126.8 or 129.7 kJ/mol respectively in regard to Fe_tO content or Fe_tO activity calculated by using regular solution model.The reduction rate of Fe_tO in CaO-SiO_2-Al_2O_3-Fe_tO slags with graphite powder is in the range of 0.15×10^(-4)- 0.86×10^(-4) mol-O/cm^2 · s. | Yang Xuemin Huang Dianbing Kong Lingtan Yang Tianjun Xie Yusheng Wang Daguang | 1998 | Journal of Iron and Steel Research(International)1998，5，1： | 0 |
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