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| 1 | Arbuscular Mycorrhizal Symbiosis Requires a Phosphate Transceptor in the Gigaspora margarita Fungal Symbiont显示文摘陆上的脉管的植物的多数能够形成 mutualistic 协会与要求 biotrophic arbuscular mycorrhizal (AM ) 从门 Glomeromycota 的真菌。这 mutualistic 共生提供糖类给真菌,并且相互地改进植物磷酸盐举起。AM 真菌的 transporters 能通过菌丝的网络从土壤获得磷酸盐。不过, AM 真菌的磷酸盐 transporters 的精确函数,并且他们是否充当传感器或作为滋养的 transporters,在真菌的信号 transduction 仍然保持不清楚。这里,我们从为 AM 共生被要求的 Gigaspora margarita 报导高亲密关系的磷酸盐 transporter GigmPT。GigmPT 的导致主人的基因 silencing 妨碍 G 的发展。margarita 在 AM 期间共生。最重要地, GigmPT 在 G 作为磷酸盐 transceptor 工作。关于表明小径以及蛋白质的磷酸盐的激活的 margarita kinase A 发信号串联。用代用品半胱氨酸可接近性方法,我们识别了残余 A 146( 在 transmembrane 领域[TMD ] IV ) 并且瓦尔 357( 在 TMD VIII ) GigmPT,哪个为磷酸盐在生长期间在酵母发信号和运输是批评的感应。一起,我们的结果从 AM 真菌 G 提供重要卓见进磷酸盐 transceptor 的分子的功能。margarita。 | Xianan Xie Hui Lin Xiaowei Peng Congrui Xu Zhongfeng Sun Kexin Jiang Antian Huang Xiaohui Wu Nianwu Tang Alessandra Salvioli Paola Bonfante Bin Zhao | 2016 | Molecular Plant2016，9，12： | 6 |
| 2 | Molecular cloning and functional identification of sterol C24-methyltransferase gene from Tripterygium wilfordii显示文摘Sterol C24-methyltransferase(SMT) plays multiple important roles in plant growth and development. SMT1, which belongs to the family of transferases and transforms cycloartenol into 24-methylene cycloartenol, is involved in the biosynthesis of 24-methyl sterols. Here, we report the cloning and characterization of a cDNA encoding a sterol C24-methyltransferase from Tripterygium wilfordii(Tw SMT1). Tw SMT1(Gen Bank access number KU885950) is a 1530 bp cDNA with a 1041 bp open reading frame predicted to encode a 346-amino acid, 38.62 k Da protein. The polypeptide encoded by the SMT1 cDNA was expressed and purified as a recombinant protein from Escherichia coli(E. coli) and showed SMT activity. The expression of Tw SMT1 was highly up-regulated in T. wilfordii cell suspension cultures treated with methyl jasmonate(Me JA). Tissue expression pattern analysis showed higher expression in the phellem layer compared to the other four organs(leaf, stem, xylem and phloem), which is about ten times that of the lowest expression in leaf. The results are meaningful for the study of sterolbiosynthesis of T. wilfordii and will further lay the foundations for the research in regulating both the content of other main compounds and growth and development of T. wilfordii. | Hongyu Guan Yujun Zhao Ping Su Yuru Tong Yujia Liu Tianyuan Hu Yifeng Zhang Xianan Zhang Jia Li Xiaoyi Wu Luqi Huang Wei Gao | 2017 | Acta Pharmaceutica Sinica B2017，7，5： | 4 |
| 3 | Tailoring polysulfide trapping and kinetics by engineering hollow carbon bubble nanoreactors for high-energy Li-S pouch cells显示文摘Despite great progress of lithium-sulfur(Li-S)battery performance at the laboratory-level,both key parameters and challenges at cell scales to achieve practical high energy density require high-sulfur-loading cathodes and lean electrolytes.Herein,a novel carbon foam integrated by hollow carbon bubble nanoreactors with ultrahigh pore volume of 6.9 cm3·g−1 is meticulously designed for ultrahigh sulfur content up to 96 wt.%.Tailoring polysulfide trapping and ion/electron transport kinetics during the charge-discharge process can be achieved by adjusting the wall thickness of hollow carbon bubbles.And a further in-depth understanding of electrochemical reaction mechanism for the cathode is impelled by the in-situ Raman spectroscopy.As a result,the as-prepared cathode delivers high specific capacitances of 1,269 and 695 mAh·g−1 at 0.1 and 5 C,respectively.Furthermore,Li-S pouch cells with high areal sulfur loading of 6.9 mg·cm−2 yield exceptional practical energy density of 382 Wh·kg−1 under lean electrolyte of 3.5µL·mg−1,which demonstrates the great potential for realistic high-energy Li-S batteries. | Lei Wang Shuangke Liu Jin Hu Xianan Zhang Xin Li Guanhua Zhang Yujie Li Chunman Zheng Xiaobin Hong Huigao Duan | 2021 | Nano Research2021，14，5： | 3 |
| 4 | Production of high levels of soluble recombinant Streptomyces clavuligerus isopenicillin N synthase in Escherichia coli显示文摘 | B.J. Sim Doreen S.H. Tan Xianan Liu T.S. Sim | 1996 | Journal of Molecular Catalysis B Enzymatic1996，，2： | 1 |
| 5 | Cloning and characterization of a novel 3-hydroxy-3-methylglutaryl coenzyme A reductase gene from Salvia miltiorrhiza involved in diterpenoid tanshinone accumulation显示文摘 | Zhubo Dai Guanghong Cui Shu-Feng Zhou Xianan Zhang Luqi Huang | 2010 | Journal of Plant Physiology2010，，2： | 1 |
| 6 | Assessing the skill of an all-season statistical forecast model for the Madden-Julian Oscillation显示文摘 | Jiang Xianan and Duane E Waliser | 2008 | Mon Wea Rev2008，136，： | 1 |
| 7 | Analysis of Language and Culture显示文摘 | | 2000 | Journal of Foreign Language Research2000，，100： | 1 |
| 8 | Ther- modynamics research on hydrogen production from bio- mass and coal co-gasification with catalyst显示文摘 | WANG Liqun DUN Yuhuan XIANG Xianan | 2011 | InternationalJournal o{ Hydrogen Energy2011，36，11： | 1 |
| 9 | Simulations of the Eastern North Pacific Intrasea-sonal Variability in CMIP5 GCMs显示文摘 | Jiang Xianan Maloney E D Li Jui-Lin F | 2013 | Journal of Climate2013，26，11： | 1 |
| 10 | Rapid amplification of genomic DNA ends bynla III partial digestion and polynucleotide tailing显示文摘 | Xianan Liu W. Vance Baird | 2001 | Plant Molecular Biology Reporter2001，，3： | 1 |
| 11 | Transmission ratio optimization of electric vehicle powertrain显示文摘 | Wang Yuming Du Changqing Li Xianan | 2013 | Applied Mechanics and Materials2013，，： | 1 |
| 12 | Real-time imaging of single synaptic vesicles in live neurons显示文摘在荧光显微镜学的最近的进展向研究人员提供了强大的新工具设想发生在实时的细胞的过程,给研究人员一个崭新的机会探讨以前是无法接近的许多生物问题。关于神经生物学,这些即时成像技术加深了我们分子、细胞的过程的理解,包括在生活房间的单个蛋白质和细胞器的运动和动力学。在这评论,我们在实时神经原在单个 synaptic 泡的即时成像的领域里总结最近的进展。 | Chenglong Yu Min Zhang Xianan Qin Xiaofeng Yang Hyokeun Park | 2016 | Frontiers in Biology2016，11，2： | 0 |
| 13 | ARK2 stabilizes the plus-end of microtubules and promotes microtubule bundling in Arabidopsis显示文摘Microtubule dynamics and organization are important for plant cell morphogenesis and development.The microtubule-based motor protein kinesins are mainly responsible for the transport of some organelles and vesicles,although several have also been shown to regulate microtubule organization.The ARMADILLO REPEAT KINESIN(ARK)family is a plant-specific motor protein subfamily that consists of three members(ARK1,ARK2,and ARK3)in Arabidopsis thaliana.ARK2 has been shown to participate in root epidermal cell morphogenesis.However,whether and how ARK2 associates with microtubules needs further elucidation.Here,we demonstrated that ARK2 co-localizes with microtubules and facilitates microtubule bundling in vitro and in vivo.Pharmacological assays and microtubule dynamics analyses indicated that ARK2 stabilizes cortical microtubules.Live-cell imaging revealed that ARK2moves along cortical microtubules in a processive mode and localizes both at the plus-end and the sidewall of microtubules.ARK2 therefore tracks and stabilizes the growing plus-ends of microtubules,which facilitates the formation of parallel microtubule bundles. | Miao Lan Xianan Liu Erfang Kang Ying Fu Lei Zhu | 2023 | Journal of Integrative Plant Biology2023，65，1： | 0 |
| 14 | Multiple-statistical genome-wide association analysis and genomic prediction of fruit aroma and agronomic traits in peaches显示文摘‘Chinese Cling’is an important founder in peach breeding history due to the pleasant flavor.Genome-wide association studies(GWAS)combined with genomic selection are promising tools in fruit tree breeding,as there is a considerable time lapse between crossing and release of a cultivar.In this study,242 peaches from Shanghai germplasm were genotyped with 145456 single-nucleotide polymorphisms(SNPs).The six agronomic traits of fruit flesh color,fruit shape,fruit hairiness,flower type,pollen sterility,and soluble solids content,along with 14 key volatile odor compounds(VOCs),were recorded for multiple-statistical GWAS.Except the reported candidate genes,six novel genes were identified as associated with these traits.Thirty-nine significant SNPs were associated with eight VOCs.The putative candidate genes were confirmed for VOCs by RNA-seq,including three genes in the biosynthesis pathway found to be associated with linalool,soluble solids content,and cis-3-hexenyl acetate.Multiple-trait genomic prediction enhanced the predictive ability forγ-decalactone to 0.7415 compared with the single-trait model value of 0.1017.One PTS1-SSR marker was designed to predict the linalool content,and the favorable genotype 187/187 was confirmed,mainly existing in the‘Shanghai Shuimi’landrace.Overall,our findings will be helpful in determining peach accessions with the ideal phenotype and show the potential of multiple-trait genomic prediction to improve accuracy for highly correlated genetic traits.The diagnostic marker will be valuable for the breeder to bridge the gap between quantitative trait loci and marker-assisted selection for developing strong-aroma cultivars. | Xiongwei Li Jiabo Wang Mingshen Su Minghao Zhang Yang Hu Jihong Du Huijuan Zhou Xiaofeng Yang Xianan Zhang Huijuan Jia Zhongshan Gao Zhengwen Ye | 2023 | Horticulture Research2023，10，7： | 0 |
| 15 | From liver fibrosis to hepatocarcinogenesis: Role of excessive liver H_(2)O_(2) and targeting nanotherapeutics显示文摘Liver fibrosis and hepatocellular carcinoma(HCC)have been worldwide threats nowadays.Liver fibrosis is reversible in early stages but will develop precancerosis of HCC in cirrhotic stage.In pathological liver,excessive H_(2)O_(2) is generated and accumulated,which impacts the functionality of hepatocytes,Kupffer cells(KCs)and hepatic stellate cells(HSCs),leading to genesis of fibrosis and HCC.H_(2)O_(2) accumulation is associated with overproduction of superoxide anion(O_(2)^(·-))and abolished antioxidant enzyme systems.Plenty of therapeutics focused on H_(2)O_(2) have shown satisfactory effects against liver fibrosis or HCC in different ways.This review summarized the reasons of liver H_(2)O_(2) accumulation,and the role of H_(2)O_(2) in genesis of liver fibrosis and HCC.Additionally,nanotherapeutics targeting H_(2)O_(2) were summarized for further consideration of antifibrotic or antitumor therapy. | Meiyu Shao Yifan Wang Hongyan Dong Lu Wang Xiaoqing Zhang Xin Han Xianan Sang Yini Bao Mengyun Peng Gang Cao | 2023 | Bioactive Materials2023，，5： | 0 |
| 16 | First Observation of Yrast Band in N=91 Odd-odd ^(162)Lu Nucleus 显示文摘First Observation of Yrast Band in N=91 Odd-odd ^(162)Lu Nucleus￥ZhangYuhu;ZhouXiaohong;ZhaoQingzhong;SunXiangfu;LeiXiangguo;?.. | Zhang Yuhu Zhou Xiaohong Zhao Qingzhong Sun Xiangfu Lei Xiangguo Guo Yingxiang Liu Zhong Chen Xingfeng Zhu Yongtai Wen Shuxian Yuan Guanjun and Liu Xianan(Chinese Institute of Atomic Energy,Beijing.) | 1994 | IMP & HIRFL Annual Report1994，，0： | 0 |
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