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1篇 您的检索式：作者名="Xianggan Li"

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		题名	作者	年代	出处	被引量
	1	Developing an efficient and visible prime editing system to restore tobacco 8-hydroxy-copalyl diphosphate gene for labdane diterpene Z-abienol biosynthesis显示文摘Prime editing(PE)is a versatile CRISPR-Cas based precise genome-editing platform widely used to introduce a range of possible base conversions in various organisms.However,no PE systems have been shown to induce heritable mutations in tobacco,nor in any other dicot.In this study,we generated an efficient PE system in tobacco that not only introduced heritable mutations,but also enabled anthocyanin-based reporter selection of transgene-free T_(1) plants.This system was used to confer Zabienol biosynthesis in the allotetraploid tobacco cultivar HHDJY by restoring a G>T conversion in the NtCPS2 gene.High levels of Z-abienol were detected in the leaves of homozygous T_(1) plants at two weeks after topping.This study describes an advance in PE systems and expands genome-editing toolbox in tobacco,even in dicots,for use in basic research and molecular breeding.And restoring biosynthesis of Z-abienol in tobacco might provide an efficient way to obtain Z-abienol in plants.	Jianduo Zhang Lu Zhang Chengwei Zhang Yongxing Yang Huayin Liu Lu Li Shengxue Zhang Xianggan Li Xinxiang Liu Ya Liu Jin Wang Guangyu Yang Qingyou Xia Weiguang Wang Jinxiao Yang	2023	Science China(Life Sciences)2023，66，12：	1

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