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| 1 | Binding Activity Difference of Anti-CD20 scFv-Fc Fusion Protein Derived from Variable Domain Exchange显示文摘Two novel engineered antibody fragments binding to antigen CD20 were generated by fusing a murine IgM-type anti-CD20 single-chain Fv fragment (scFv) to the human IgG1 CH2 (i.e., Cγ2) and CH3 (i.e., Cγ3) domains with the human IgG1 hinge (i.e. Hγ). Given the relationship between structure and function of protein, the 3-D structures of the two engineered antibody fragments were modeled using computer-aided homology modeling method. Furthermore, the relationship between 3-D conformation and their binding activity was evaluated theoretically. Due to the change of active pocket formed by CDRs, the HL23 (VH-Linker-VL-Hγ-Cγ2-Cγ3) remained its activity because of its preserved conformation, while the binding activity of the LH23 (VL-Linker-VH-Hγ-Cγ2-Cγ3) was impaired severely. Experimental studies by flow cytometry and fluorescence microscopy showed that HL23 possessed significantly superior binding activity to CD20-expressing target cells than LH23. That is to say, the order of variable regions could influence the binding activity of the fusion protein to CD20+ cell lines, which was in accordance with the theoretical results. The study highlights the potential relationship between the antibody binding activity and their 3-D conformation, which appears to be worthwhile in providing direction for future antibody design of recombinant antibody. | Shusheng Geng Jiannan Feng Yan Li Yingxun Sun Xin Gu Ying Huang Yugang Wang Xianjiang Kang Hong Chang Beifen Shen | 2006 | Cellular & Molecular Immunology2006，3，6： | 13 |
| 2 | Effects of lateral ventricular transplantation of bone marrow-derived mesenchymal stem cells modified with brain-derived neurotrophic factor gene on cognition in a rat model of Alzheimer's disease显示文摘In the present study, transplantation of bone marrow-derived mesenchymal stem cells modified with brain-derived neurotrophic factor gene into the lateral ventricle of a rat model of Alzheimer's disease, resulted in significant attenuation of nerve cell damage in the hippocampal CA1 region. Furthermore, brain-derived neurotrophic factor and tyrosine kinase B mRNA and protein levels were significantly increased, and learning and memory were significantly improved. Results indicate that transplantation of bone marrow-derived mesenchymal stem cells modified with brain-derived neurotrophic factor gene can significantly improve cognitive function in a rat model of Alzheimer's disease, possibly by increasing the levels of brain-derived neurotrophic factor and tyrosine kinase B in the hippocampus. | Ping Zhang Gangyong Zhao Xianjiang Kang Likai Su | 2012 | Neural Regeneration Research2012，7，4： | 8 |
| 3 | HSP70 decreases receptor-dependent phosphorylation of Smad2 and blocks TGF-β-induced epithelial-mesenchymal transition显示文摘Smad2 和 Smad3,转变发信号的生长因素(TGF-) 的细胞内部的调停人,直接由激活的类型的 phosphorylated 我从进原子核到的细胞质的受体 kinase,然后梭调整目标基因表示。这里,我们报导 70-kDa 热吃惊蛋白质(HSP70 ) 与 Smad2 和减少 TGF- 信号 transduction 交往。HSP70 的宫外的表示阻止受体依赖者 phosphorylation 和 Smad2 的原子 translocation,并且堵住 TGF -- 在 HaCat 房间的导致的上皮间充质的转变(EMT ) 。我们的调查结果在 TGF 揭示 HSP70 的一个必要角色 -- 由阻碍 Smad2 phosphorylation 的导致的上皮间充质的转变(EMT ) 。 | Yihao Li Xianjiang Kang Qiang Wang | 2011 | Journal of Genetics and Genomics2011，38，3： | 5 |
| 4 | Impact of gasoline engine deposits on light duty vehicle emissions: in-use case study in Beijing, China显示文摘从轻型的汽油车辆的 Tailpipe 排出物通常随着时间的过去败坏。由于不适当的汽油,去垢性被考虑是如此的排放恶化的主要原因之一的引擎存款的累积。六在里面使用在北京的轻型的汽油车辆被测试在烃(HC ) 的排出物上调查引擎存款的影响,碳一氧化物(公司) 和氮氧化物(没有 x ) 。在引擎存款被移开前后,在从六轻责任车辆的冷开始和热跑测试条件下面的排出物被测量。结果证明尽管单个车辆为每污染物质不同地反应了,引擎存款的消除平均在 29.4% 在热跑的条件下面减少了 HC 排出物,在在 23.0% 的冷开始条件下面的公司排出物和在在 35.5% 的热跑的条件下面的公司排出物(t < 0.05 在所有情况中) 。没有污染物质排出物与统计意义增加了(t < 0.05 ) 在引擎存款的移动以后。在引擎存款的移动之上的排放变化的变化被观察。如此的变化与以前的研究一致,暗示车辆排出物上的引擎存款的影响模式可以服从于充分没被理解的许多影响因素并且对在所有条件下面的控制困难。车辆排出物上的引擎存款的影响的一个统计看法可能为越过一个城市或一个乡村的排出物减小的评估是适当的。在实践维持足够、有效的汽油燃料去垢性使引擎干净并且接着减少车辆排出物是必要的。 | Xin YUE Ye WU Xianjiang HUANG Yao MA Yuan PANG Xiaofeng BAO Jiming HAO | 2012 | Frontiers of Environmental Science & Engineering2012，6，5： | 3 |
| 5 | Process optimization,texture and microstructure of novel kelp tofu显示文摘Laminaria japonica Aresch as an edible and medicine dual-purpose marine algae,has been gradually accepted by people.Tofu was Chinese traditional food,combining kelp function ingredients with tofu has practical value.The extraction-optimization methodology and the sequences of kelp antihypertensive peptide(KAP)were investigated.The sensory,whiteness,water retention ability(WRA)and microstructure of KAP tofu were evaluated.The scanning electron microscope(SEM)results showed KAP addition(20%)significantly improved the tofu texture,with a denser network composed of relatively even pores.Compared with gypsum tofu and glucose-δ-interior fat(GDL)tofu,KAP tofu had significantly higher WRA(94.49±0.49)%and sensory evaluation score value(92.54±0.52),and a significantly lower IC_(50) value at(2.06±0.04)mg/mL(P<0.05).The maximum IC_(50) value(4.14 mg/mL)of kelp enzymatic hydrolysate was observed at enzyme(at an alkaline protease:trypsin ratio of 2:1)concentration 1.5%,temperature 55℃ and time 2 h.The sequences of 8 identified peptides were Lys-Tyr,Phe-Tyr,Gly-Lys-Tyr,Ala-Lys-Tyr,Ser-Lys-Thr-Tyr,Lys-Lys-Phe-Tyr,Lys-Phe-Lys-Tyr and Ala-Lys-Tyr-Ser-Tyr with IC_(50) values of 5.24,4.83,7.94,7.52,20.63,15.33,10.73 and 2.42μmol/L,respectively.These results indicated the potential use of KAP tofu as a valuable resource for development of functional foods. | Xianjiang Ye Li Chen Zhichen Su Xiaojuan Lin Jicheng Chen | 2023 | Food Science and Human Wellness2023，12，1： | 2 |
| 6 | A parabolic quasi-variational inequlity connected with non-stationary filtration problem显示文摘 | Bai Donghua Tang Xianjiang | 1985 | Chinese Annals of Mathematics1985，6，6： | 1 |
| 7 | Role of X-linked inhibitor of apoptosis protein in chemoresistance in ovarian cancer: possible involvement of the phosphoinositide-3 kinase/Akt pathway显示文摘 | Jin Q Cheng C Xiu Xianjiang | 2002 | Drug Resistance Updates2002，5，3： | 1 |
| 8 | A monoclonal antibody targeting neuropilin-1 inhibits adhesion of MCF7 breast cancer cells to fibronectin by suppressing the FAK/p130cas signaling pathway显示文摘 | Fanwei Zeng Fanghong Luo Sha Lv Haipeng Zhang Chang Cao Xiaoli Chen Shengyu Wang Zhe Li Xianjiang Wang Xiaofeng Dou Yujuan Dai Mingjun He Yafei Zhang Haiyan Lv Jianghua Yan Yuqiang Chen | 2014 | Anti-Cancer Drugs2014，，： | 1 |
| 9 | Solid‐phase extraction with the metal–organic framework MIL ‐101( C r) combined with direct analysis in real time mass spectrometry for the fast analysis of triazine herbicides<link href='#jssc3723-note-0002'/>显示文摘 | Xianjiang Li Jiawei Xing Cuilan Chang Xin Wang Yu Bai Xiuping Yan Huwei Liu | 2014 | J Sep Science2014，，12： | 1 |
| 10 | Human IgG1 C_γ1 Domain Is Crucial for the Bioactivity of the Engineered Anti-CD20 Antibodies显示文摘In this study, we discussed the necessity of human IgG1 Cγ1 domain for recombinant antibody using computeraided homology modeling method and experimental studies. The heavy (VH) and light (VL) chain variable regions of 1-28, a murine IgM-type anti-CD20 mAb, were ligated by linker peptide (Gly4Ser)3 to form the single-chain Fv fragment (scFv). Then, the engineered antibody (LH1-3) was generated by fusing scFv with the entire IgG1 heavy constant regions. The 3-D structure of LH1-3 was modeled using computer-aided homology modeling method and the binding activity of LH1-3 was evaluated theoretically. Compared to the 3-D structure of the Fv fragment of the parent antibody, the conformation of the active pocket of LH1-3 was remained because of the rigid support of Cγ1. Further experimental results of flow cytometry showed that the engineered anti-CD20 antibody possessed specifically binding activity to CD20-expressing target cells. The anti-CD20 antibody fragments could also mediate complement-dependent cytotoxicity (CDC) of human B-lymphoid cell lines. Our study highlights some interests and advantages of a methodology based on the homology modeling and analysis of molecular structural properties. | Shusheng Geng Jiannan Feng Yan Li Xianjiang Kang Yingxun Sun Xin Gu Ying Huang Hong Chang Beifen Shen | 2007 | Cellular & Molecular Immunology2007，4，2： | 1 |
| 11 | Antisense hypoxia‐inducible factor 1α gene therapy enhances the therapeutic efficacy of doxorubicin to combat hepatocellular carcinoma显示文摘 | FengjunLiu PeijunWang XianJiang GangTan HaiquanQiao HongchiJiang Geoffrey W.Krissansen XueyingSun | 2008 | Cancer Science2008，，10： | 1 |
| 12 | Novel piperidine nitroxide derivatives synthesis, electrochemical and antioxidative evaluation显示文摘 | Zhao Hongli Wu Juhua Meng Xianjiang | 2008 | J Heterocyclic Chem2008，45，： | 1 |
| 13 | An organic-inorganic hybrid thermochromic ferroelastic with multi-channel switches显示文摘Multifunctional switchable materials are attracting tremendous interest because of their great application potential in signal processing,information encryption,and smart devices.Here,we reported an organic-inorganic hybrid thermochromic ferroelastic crystal,[TMIm][CuCl_(4)](TMIm=1,1,3,3-tetramethylimidazolidinium),which undergoes two reversible phase transitions at 333 K and 419 K,respectively.Intriguingly,these three phases experience a remarkable ferroelastic-paraelastic-ferroelastic(2/m-mmm-2/m)transition,which remains relatively unexplored in ferroelastics.Moreover,the ferroelastic domains can be simultaneously switched under temperature and stress stimuli.Meanwhile,[TMIm][CuCl_(4)]exhibits thermochromic phenomenon,endowing it with extra spectral encryption possibilities during information processing.Combined with dielectric switching behavior,[TMIm][Cu Cl_(4)]are promising for practical applications in memory devices,next-generation sensors,and encryption technology. | Yipiao Zeng Junchao Liu Lin Zhou Xin Deng Wenli Yang Xin Yan Yanling Luo Xuan Zhu Xiaoyun Huang Xianjiang Song Yuanyuan Tang | 2023 | Chinese Chemical Letters2023，34，9： | 0 |
| 14 | Seasonal variations in the composition and functional profiles of gut microbiota reflect dietary changes in plateau pikas显示文摘Seasonal variations in gut microbiota of small mammals and how they are influenced by environmental variables are relatively poorly understood.We sampled 162 wild plateau pikas(Ochotona curzoniae)in 4 seasons over 2 and a half years and recorded the air temperature,precipitation,and nutrient content in edible vegetation at the sampling site.After conducting 16S rRNA and shotgun metagenomic sequencing,we found that the highest alpha diversity,the relative abundance of Firmicutes,and the simplest co-occurrence network occurred in winter,whereas the highest relative abundance of Proteobacteria and the most complex network structure were observed in spring.The highest relative abundance of Verrucomicrobiota and Spirochaetota was seen in summer and autumn,respectively.Air temperature,precipitation,and the contents of crude protein,crude fiber,and polysaccharide in vegetation had significant effects on the seasonal changes in gut microbiota.Diet contributed more to microbial variation than climatic factors.Metagenomic analysis revealed that the amino acid metabolism pathway and axillary activity enzymes were most abundant in summer,while abundance of carbohydrate-binding modules and carbohydrate esterases was highest in spring.These microbial variations were related to the changes in dietary nutrition,indicating that gut microbiota of plateau pika contribute to the efficient use of food resources.This study provides new evidence of how external environmental factors affect the intestinal environment of small mammals. | Chao FAN Liangzhi ZHANG Shangang JIA Xianjiang TANG Haibo FU Wenjing LI Chuanfa LIU He ZHANG Qi CHENG Yanming ZHANG | 2022 | Integrative Zoology2022，17，3： | 0 |
| 15 | Preparation of Ammonium Tungstophosphate-Calcium Alginate Composite Adsorbent and the Separation of Rubidium and Potassium显示文摘As a kind of rare metals,rubidium is often used to prepare special glass,photomultiplier tubes,thermoelectric converter,organic catalysts and antidepressants.Rubidium forms no minerals of its own,hence,it often coexists | AN Lianying HUANG Xianjiang ZHAO Xianyin ZHANG Chunxia HUANG Zhenggen | 2014 | Acta Geologica Sinica(English Edition)2014，88，S1： | 0 |
| 16 | L^p-L^q ESTIMATES FOR HIGHER ORDER PERTURBED HYPERBOLIC EQUATION显示文摘Lp┐LqESTIMATESFORHIGHERORDERPERTURBEDHYPERBOLICEQUATIONMUCHUNLAIANDTANGXIANJIANGAbstract.InthispaperLp-Lqestimatesforthesolut... | MU CHUNLAI AND TANG | 1998 | Applied Mathematics(A Journal of Chinese Universities)1998，13，1： | 0 |
| 17 | Fabrication of 2×2 Thermo-Optic Switches with Organic-Inorganic Hybrid Materials Prepared by Sol-Gel Technique显示文摘2×2 Mach-Zehnder interferometric thermo-optic switch was fabricated with organic/inorganic hybrid materials by sol-gel technique and direct UV patterning. The switching time of device was measured to be 4.2 ms and switching power 9.3 mW. | Xianjiang Wang, Lei Xu, Dongxiao Li, Liying Liu, and Wencheng WangState Key Lab for Advanced Photonic Materials and Devices, Department of Optical Science and Engineering, Fudan University, Shanghai 200433, P. R. China | 2003 | 光学学报2003，23，S1： | 0 |
| 18 | Accurate Quantification of Pure Thiacloprid with Mass Balance and Quantitative H-NMR显示文摘Thiacloprid is the first-generation neonicotinoid insecticide,which is widely used in modern agriculture.Reference materials are essential for sound measurement and traceability of results in chemical analysis.Pure thiacloprid was comprehensively assessed by two orthonormal methods:mass balance(MB) and quantitative nuclear magnetic resonance spectroscopy(qNMR).From MB,its mass fraction value was 998.4 mg/g with the subtraction of structurally related impurities(99.87%),inorganic impurities(0.006 mg/g),water(0.33 mg/g),and volatile organic solvent(0 mg/g).To guarantee sufficient separation of structurally related purity,peak purity of thiacloprid was evaluated by five points of UV spectra.From the qNMR of hydrogen,its mass fraction value was 995.7 mg/g which was traced to the internal standard(methyl sulfone).Finally,the certified value was assigned to 997 mg/g,together with an expanded uncertainty of 3 mg/g(k=2).This high-purity thiacloprid can be applied to routine monitoring in agriculture and the food fields. | Mengling Tu Wei Zhang Yunxiao Zhu Wen Ma Xiao Li Shukun Zhou Hongmei Li Xianjiang Li | 2024 | Journal of Analysis and Testing2024，8，1： | 0 |
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