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| 1 | Evolution of the novel coronavirus from the ongoing Wuhan outbreak and modeling of its spike protein for risk of human transmission显示文摘Dear Editor,The occurrence of concentrated pneumonia cases in Wuhan city,Hubei province of China was first reported on December 30,2019 by the Wuhan Municipal Health Commission(WHO,2020).The pneumonia cases were found to be linked to a large seafood and animal market in Wuhan,and measures for sanitation and disinfection were taken swiftly by the local government agency.The Centers for Disease Control and Prevention(CDC)and Chinese health authorities later determined and announced that a novel coronavirus(CoV),denoted as 2019-nCoV,had caused the pneumonia outbreak in Wuhan city(CDC,2020).Scientists from multiple groups had obtained the virus samples from hospitalized patients(Normile,2020).The isolated viruses were morphologically identical when observed under electron microscopy. | Xintian Xu Ping Chen Jingfang Wang Jiannan Feng Hui Zhou Xuan Li Wu Zhong Pei Hao | 2020 | Science China(Life Sciences)2020，63，3： | 737 |
| 2 | Binding Activity Difference of Anti-CD20 scFv-Fc Fusion Protein Derived from Variable Domain Exchange显示文摘Two novel engineered antibody fragments binding to antigen CD20 were generated by fusing a murine IgM-type anti-CD20 single-chain Fv fragment (scFv) to the human IgG1 CH2 (i.e., Cγ2) and CH3 (i.e., Cγ3) domains with the human IgG1 hinge (i.e. Hγ). Given the relationship between structure and function of protein, the 3-D structures of the two engineered antibody fragments were modeled using computer-aided homology modeling method. Furthermore, the relationship between 3-D conformation and their binding activity was evaluated theoretically. Due to the change of active pocket formed by CDRs, the HL23 (VH-Linker-VL-Hγ-Cγ2-Cγ3) remained its activity because of its preserved conformation, while the binding activity of the LH23 (VL-Linker-VH-Hγ-Cγ2-Cγ3) was impaired severely. Experimental studies by flow cytometry and fluorescence microscopy showed that HL23 possessed significantly superior binding activity to CD20-expressing target cells than LH23. That is to say, the order of variable regions could influence the binding activity of the fusion protein to CD20+ cell lines, which was in accordance with the theoretical results. The study highlights the potential relationship between the antibody binding activity and their 3-D conformation, which appears to be worthwhile in providing direction for future antibody design of recombinant antibody. | Shusheng Geng Jiannan Feng Yan Li Yingxun Sun Xin Gu Ying Huang Yugang Wang Xianjiang Kang Hong Chang Beifen Shen | 2006 | Cellular & Molecular Immunology2006，3，6： | 13 |
| 3 | Characterization of the neutralizing activity of three anti-human TNF monoclonal antibodies and prediction of their TNF epitopes by molecular modeling and mutant protein approach显示文摘 | Cansheng Zhu Xuesong Liu Jiannan Feng Wei Zhang Beifen Shen Weiming Ou'yang Yunxin Cao Boquan Jin | 2006 | 中国生物学文摘2006，20，1： | 5 |
| 4 | Potent anti-angiogenesis and anti-tumor activity of a nove human anti-VEGF antibody, MIL60显示文摘 | Jing Yang Qun Wang Chunxia Qiao Zhou Lin Xinying Li Yifei Huang Tingting Zhou Yan Li Beifen Shen Ming Lv Jiannan Feng | 2014 | Cellular & Molecular Immunology2014，11，3： | 4 |
| 5 | Selection and characterization of the novel anti-human PD-1 FV78 antibody from a targeted epitope mammalian cell-displayed antibody library显示文摘Currently,display-based methods are well established and widely used in antibody engineering for affinity maturation and structural stability improvement.We obtained a novel anti-human programmed death 1(PD-1)antibody using computer-aided design and a mammalian cell display technology platform.We used computer-aided modeling and distance geometry methods to predict and assign the key residues that contributed to the binding of human PD-L1 to PD-1.Then,we analyzed the sequence of nivolumab(an anti-human PD-1 antibody,referred to as MIL75 in the article)to determine the template for antibody design and library construction.We identified a series of potential substitutions on the obtained template and constructed a virtual epitope-targeted antibody library based on the physicochemical properties and each possible location of the assigned key residues.The virtual antibody libraries were displayed on the surface of mammalian cells as the antigen-binding fragments of full-length immunoglobulin G.Then,we used flow cytometry and sequencing approaches to sort and screen the candidates.Finally,we obtained a novel anti-human PD-1 antibody named FV78.FV78 competitively recognized the PD-1 epitopes that interacted with MIL75 and possessed an affinity comparable to MIL75.Our results implied that FV78 possessed equivalent bioactivity in vitro and in vivo compared with MIL75,which highlighted the probability and prospect of FV78 becoming a new potential antibody therapy. | Longlong Luo Shi Wang Xiaoling Lang Tingting Zhou Jing Geng Xinying Li Chunxia Qiao Jiannan Feng Beifen Shen Ming Lv Yan Li | 2018 | Cellular & Molecular Immunology2018，15，2： | 4 |
| 6 | Characterization of a Novel Anti-DR5 Monoclonal Antibody WD1 with the Potential to Induce Tumor Cell Apoptosis显示文摘TNF-related apoptosis-inducing ligand (TRAIL) is a TNF family member capable of inducing apoptosis. Death receptor 5 (DR 5) is a key receptor of TRAIL and plays an important role in TRAIL-induced apoptosis. To prepare monoclonal antibodies (mAbs) against DR5,cDNA encoding soluble DR5 (sDR5) was firstly amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) with specific primers,and then inserted into a prokaryotic expression vector pET-30a. The recombinant plasmid was expressed in Escherichia coli strain BL21 (DE3),and sDR5 was purified by nickel affinity chromatography. As an antigen,sDR5 was used to immunize mice. Hybridomas secreting antibodies against sDR5 were identified. One positive clone was selected to produce antibody,WD1. ELISA and immunofluorescence demonstrated that WD1 could bind recombinant sDR5 and membrane-bound DR5 (mDR5) on Jurkat and Molt-4 cells. ATPLite assays showed that Jurkat and Molt-4 cells were sensitive to the antibody in a dose dependent manner. The Annexin V/PI assays and Giemsa's staining both showed that WD1 could induce Jurkat cell apoptosis efficiently. Transient transfection of 293T cells and indirect immunofluorescence assay demonstrated that mAb (WD1) couldn't cross-react with DR4. Our findings indicated that the novel antibody,WD1 could act as a direct agonist,bind DR5 characteristically,and initiate efficient apoptotic signaling and tumor regression. Thus,WD1 would be a leading candidate for potential cancer therapeutics. | Jing Wang Zhou Lin Chunxia Qiao Ming Lv Ming Yu He Xiao Qingyang Wang Liyan Wang Jiannan Feng Beifen Shen Yuanfang Ma Yan Li | 2008 | Cellular & Molecular Immunology2008，5，1： | 4 |
| 7 | Hyperthermia based individual in situ recombinant vaccine enhances lymph nodes drainage for de novo antitumor immunity显示文摘The continuing challenges that limit effectiveness of tumor therapeutic vaccines were high heterogeneity of tumor immunogenicity, low bioactivity of antigens, as well as insufficient lymph nodes(LNs) drainage of antigens and adjuvants. Transportation of in situ neoantigens and adjuvants to LNs may be an effective approach to solve the abovementioned problems. Therefore, an FA-TSL/AuNCs/SV nanoplatform was constructed by integrating simvastatin(SV) adjuvant loaded Au nanocages(AuNCs)as cores(AuNCs/SV) and folic acid modified thermal-sensitive liposomes(FA-TSL) as shells to enhance de novo antitumor immunity. After accumulation in tumor guided by FA, AuNCs mediated photothermal therapy(PTT) induced the release of tumor-derived protein antigens(TDPAs) and the shedding of FATSL. Exposed AuNCs/SV soon captured TDPAs to form in situ recombinant vaccine(AuNCs/SV/TDPAs). Subsequently, AuNCs/SV/TDPAs could efficiently transport to draining LNs owing to the hyperthermia induced vasodilation effect and small particle size, achieving co-delivery of antigens and adjuvant for initiation of specific T cell response. In melanoma bearing mice, FA-TSL/AuNCs/SV and laser irradiation effectively ablated primary tumor, against metastatic tumors and induced immunological memory. This approach served a hyperthermia enhanced platform drainage to enable robust personalized cancer vaccination. | Cuixia Zheng Xinxin Liu Yueyue Kong Lei Zhang Qingling Song Hongjuan Zhao Lu Han Jiannan Jiao Qianhua Feng Lei Wang | 2022 | Acta Pharmaceutica Sinica B2022，12，8： | 2 |
| 8 | Active regions' setting of the extracellular ligand-binding domain of human interleukin-6 receptor显示文摘The reliable three dimensional (3-D) structure of the extracellular ligand-binding domain (V106-P322) of human interleukin-6 receptor (hlL-6R) has been constructed by means of computer-guided homology modeling techniques using the crystal structure of the extracellular ligand-binding region (K52-L251) of human growth hormone receptor (hGHR) as templet. The space location of some key residues which influence the combination ability between the receptor and the ligand has been observed and the effects of point mutagenesis of the four conservative cysteine residues on the space conformation are analyzed. The results show that the space conformation of the side-chain carboxyl of E305 plays a key role in the ligand-binding ability. Furthermore, the space conformation of the side-chain carboxyl of E305 is very important for the electrostatic potential complementarity between hlL-6R and hlL-6 according to the docking method. | Yunfang Ren Hong Qu Jiannan Feng Song Li Beifen Shen | 2000 | Chinese Science Bulletin2000，45，13： | 2 |
| 9 | Identification of binding epitope of a monoclonal antibody (Z12) against human TNF-α using computer modeling and deletion mutant technique显示文摘The genes of the heavy and light chain variable region (VH, VL) of Z12 antibody against hTNF-a were cloned, and according to the translated sequence of amino acids, the spa-tial structures of VH and VL domains were modeled by using homology-based modeling method, followed by constructing the whole three-dimensional structure of Fv fragment. The complex model of Fv interacting with hTNF-a was gained with computer-guided molecular docking method, based on which, it was predicted that the epitope recognized by Z12 was from 141 to 146 of hTNF-a. hTNF-a molecule was divided into two fragments of N-terminal region from 1 to 91 and C-terminal region from 92 to 157 with prokaryotic expression. The measured results suggested that the antigenic epitope recognized by Z12 antibody was located in the C-terminal region 92-157 of hTNF-a, proving the predicted result reliable preliminarily. Further experimental results showed that after hTNF- 141-146 residues were deleted, Z12 antibody almost lost the ability to recognize the mutant, suggesting that the amino acid residues from 141 to 146 of hTNF-a were specially recognized by Z12 antibody. | ZHANG Wei FENG Jiannan SHEN Beifen | 2004 | Science China(Life Sciences)2004，47，3： | 2 |
| 10 | Trie-join: efficient trie-based string similarity joins with edit-distance constraints 显示文摘 | WANG Jiannan FENG Jianhua LI Guoliang | 2010 | Proceedings of the VLDB Endowment2010，3，1： | 1 |
| 11 | Molecular modeling and affinity determination of scFv antibody: proper linker peptide enhances its activity显示文摘 | Xin Gu Xiaoling Jia Jiannan Feng | 2010 | Ann Biomed Eng2010，38，2： | 1 |
| 12 | C-terminus of TRAP in Staphylococcus can enhance the activity of lysozyme and lysostaphin显示文摘在葡萄球菌 aureus,激活蛋白质(陷井) 的 RNAIII 的目标是其 C 终点能被用作一支疫苗对 staphylococcal 感染提供保护的联系膜的蛋白质。这里,我们由表面电浆子第一次证明回声和套住的连接酶的 immunosorbent 试金能明确地通过它的 C 终点(氨基酸 155-167 ) 绑溶解酵素和 lysostaphin 并且在 vitro 提高 lysozomal 活动。陷井异种紧张对 lysostaphin 更抵抗比,这也被发现野类型。我们的以前的数据证明陷井力量的 C 终点是细胞外的。我们的结果因此建议陷井的 C 终点能对 S 作为 lysozyme/lysostaphin 的特定的指向蛋白质起作用。aureus 房间墙和相互作用的生物意义可能是便于 lysozyme/lysostaphin-mediated 房间细胞溶解。 | Guang Yang Yaping Gao Jiannan Feng Yong Huang Shaohua Li Yu Liu Chuan Liu Ming Fan Beifen Shen Ningsheng Shao | 2008 | Acta Biochimica et Biophysica Sinica2008，40，5： | 1 |
| 13 | The N- and C-terminal carbohydrate recognition domains of galectin-9 contribute differently to its multiple functions in innate immunity and adaptive immunity显示文摘 | Yurong Li Jiannan Feng Shaoxia Geng Shusheng Geng Huawei Wei Guojiang Chen Xinying Li Liyan Wang Renxi Wang Hui Peng Gencheng Han Beifen Shen Yan Li | 2010 | Molecular Immunology2010，，4： | 1 |
| 14 | Tile-join: efficient Trie-based string similarity joins with edit-distance con- straints显示文摘 | Wang Jiannan Feng Jianhua Li Guoliang | 2010 | Proceedings of the VLDB Endowment2010，3，12： | 1 |
| 15 | Calculated properties of cationic phosphorus clusters P+2n+1 with n=3,4,5显示文摘 | Feng Jiannan Cui Meng Huang Xuri | 1998 | J Mol Struct (Theochem)1998，425，： | 1 |
| 16 | Blast responses of shallow-buried prefabricated modular concrete tunnels reinforced by BFRP-steel bars显示文摘This paper reports the anti-blast performance of shallow-buried prefabricated modular tunnel reinforced by basalt fiber-reinforced polymer(BFRP)-steel bars.Three concrete arch members with steel bars and three concrete arch members with BFRP-steel bars were fabricated,with the other arch parameters kept constant.The three identical arches were assembled into an integral structure and then buried in soil for field anti-blast experiments.Through the experiment,the pressure on the vault,the displacement and acceleration of the vault,the strain in the reinforcement bars and the macroscopic damage of the arches under the blast load were determined.To evaluate the damage of the arch tunnel,a residual load-bearing capacity test was conducted on the arch members after the explosion experiment.The experimental results showed that the BFRP-steel bars reinforced concrete arch exhibited a higher load-bearing capacity and more safety redundancy than the steel bars reinforced concrete arch,and that the BFRP-steel bars could inhibit the occurrence of concrete cracks to a certain extent.A comparison between the arches assembled at different positions showed that the prefabricated modular tun-nel can be simplified directly as a two-dimensional arch structure under the blast load for analysis and calculation. | Chengjie Zhao Zexun Tang Peng Wang Jiang Feng Jiannan Zhou Xinli Kong Hansheng Geng Fengnian Jin Hualin Fan | 2022 | Underground Space2022，7，2： | 1 |
| 17 | A Novel Neutralizing Monoclonal Antibody against Cell-binding polypeptide of Ricin 显示文摘 | Jianwei Guo Beifen Shen Jiannan Feng | 2005 | Hybridoma2005，24，5： | 1 |
| 18 | Thermal fatigue of ingot mould显示文摘 | FENG Junyi CHEN Jiannan | 1988 | Journal of Beijing University of Iron and Steel Technology1988，10，4： | 1 |
| 19 | Construction of 3D structure of scFv against the human placental acidic isoferritin显示文摘 | Zhou Chun Feng Jiannan Shen Guanxin | 2000 | J Tongji Med Univ2000，20，1： | 1 |
| 20 | Trie-join: Efficienttrie-based string similarity joins with edit-distance constraints 显示文摘 | Wang Jiannan Feng Jianhua Li Guoliang | 2010 | Proceedings of the VLDBEndowment2010，3，12： | 1 |
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