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| 1 | Comparison of anti-atherosclerotic effects of two different extracts from leaves of Mallotus furetianus显示文摘Objective:To compare the anti-atherosclerotic effects of two different extracts from the leaves of Mallotus fiiretianus by using rat model of atherosclerosis.Methods:The air-flriefl powdered Mallotus fiiretianus leaves were extracted with ethanol and then evaporated.The ethanol extract was experienced Diaion HP-20 CC with a gradient of MeOH and H2O(50:50,100:0,v/v) and two fractions,Mallotus fiiretianus A(Mf A) and Mallotus fiiretianus B(MfB) were obtained.RaLs were divided into control,atherosclerosis and vitamin E,Mf A and Mf B treated groups.Atherosclerotic model was established by administering u loading dose of vitamin D3 and feeding standard diet enriched with 2%cholesterol,0.5%porcine chelate,0.2%methimazole,5%sugar,10%pork fat. Vitamin E(0.20 g/kg),Mf A(0.053 g/kg),Mf B(0.057 g/kg)(with the potential) were administered to interfere with the development of atherosclerosis.After 9 weeks,rats were sacrificed and the blood lipid as well as composition of bile was examined.In addition,the thoracic aorta was harvested to evaluate histological changes and the intima-media thickness ratio.Results: Atherosclerosis model was successfully established,administration of vitamin E,Mf A and Mf B increased excretion of total bilirubin in bile,decreased triglycridc(TO),total cholesterol(TC), low density lipoprotein-cholesterol(LDL-C) level,enhanced ratio of high density lipoprotein-cholesterol and LDL-C in blood,improved histological changes and diminished intimamedia thickness ratio of thoracic aorta in atherosclerotic rats.As for the difference in antiatherosclerotic effects between Mf A and MfB,Mf A may be more powerful in declining TG level and Mf B may be more effective in decreasing TC level.Conclusions:The two different extracts. Mf A and Mf B can prevent the development of atherosclerosis.In detail,Mf A is more effective in regulating TG level and Mf B is more powerful in modulating TC.level in atherosclerotic rats. | Liu Yueli Wang Liqun Wu Haitao Lin Lianbo Yi Xinan | 2011 | Asian Pacific Journal of Tropical Medicine2011，4，11： | 15 |
| 2 | Genome editing technology and application in soybean improvement显示文摘Soybean(Glycine max)is a legume crop with great economic value that provides rich protein and oil for human food and animal feed.In order to cope with the ever-increasing need for soybean products and the changing environment,soybean genetic improvement needs to be accelerated.In recent years,the rapid developed genome editing technologies,such as zinc finger nuclease(ZFNs),transcription activator-like effector nucleases(TALENs),and clustered regularly interspaced short palindromic repeats/CRISPR associated protein(CRISPR/Cas),have shown broad application prospects in gene function research and improvement of important agronomic traits in many crops,and has also brought opportunities for soybean breeding.Here we systematically reviewed recent advances in genome editing technology.We also summarized the significances,current applications,challenges and future perspectives in soybean genome editing,which could provide references for exerting the feature and advantage of this technology to better soybean improvement. | Aili Bao Chanjuan Zhang Yi Huang Haifeng Chen Xinan Zhou Dong Cao | 2020 | Oil Crop Science2020，5，1： | 7 |
| 3 | Differential expression of microRNAs in dorsal root ganglia after sciatic nerve injury显示文摘This study investigated the possible involvement of microRNAs in the regulation of genes that participate in peripheral neural regeneration.A microRNA microarray analysis was conducted and 23 microRNAs were identified whose expression was significantly changed in rat dorsal root ganglia after sciatic nerve transection.The expression of one of the downregulated microRNAs,microRNA-214,was validated using quantitative reverse transcriptase-PCR.MicroRNA-214 was predicted to target the 3′-untranslated region of Slit-Robo GTPase-activating protein 3.In situ hybridization verified that microRNA-214 was located in the cytoplasm of dorsal root ganglia primary neurons and was downregulated following sciatic nerve transection.Moreover,a combination of in situ hybridization and immunohistochemistry revealed that microRNA-214 and Slit-Robo GTPase-activating protein 3 were co-localized in dorsal root ganglion primary neurons.Western blot analysis suggested that Slit-Robo GTPase-activating protein 3 was upregulated in dorsal root ganglion neurons after sciatic nerve transection.These data demonstrate that microRNA-214 is located and differentially expressed in dorsal root ganglion primary neurons and may participate in regulating the gene expression of Slit-Robo GTPase-activating protein 3 after sciatic nerve transection. | Anjie Lu Zufa Huang Chaoyue Zhang Xianfang Zhang Jiuhong Zhao Haiying Zhang Quanpeng Zhang Song Wu Xinan Yi | 2014 | Neural Regeneration Research2014，9，10： | 5 |
| 4 | Puerarin protects Alzheimer’s disease neuronal cybrids from oxidant-stress induced apoptosis by inhibiting pro-death signaling pathways显示文摘 | Haiying Zhang Yiheng Liu Meili Lao Zhijian Ma Xinan Yi | 2010 | Experimental Gerontology2010，，： | 1 |
| 5 | An Innovative State-of-charge Estimation Method of Lithium-ion Battery Based on 5th-order Cubature Kalman Filter显示文摘The lithium-ion batteries have drawn much attention as the major energy storage system.However,the battery state estimation still suffers from inaccuracy under dynamic operational conditions,with the unstable environmental noise influencing the robustness of estimation.This paper presents a 5th-order cubature Kalman filter with improvements on adaptivity for real-time state-of-charge estimation.The second-order equivalent circuit model is developed for describing the characteristics of battery,and parameter identification is carried out according to particle swarm optimization.The developed method is validated in stable and dynamic conditions,and simulation results show a satisfactory consistency with the experimental results.The maximum estimation error under static conditions is less than 3%and the maximum error under dynamic conditions is 5%.Numerical analysis indicates that the method has better convergence and robustness than the traditional method under the disturbances of initial error,which demonstrates the potential for EV applications in harsh environments.The proposed method shows application potential for both online estimations and cloud-computing system,indicating its diverse application prospect in electric vehicles. | Huang Yi Shichun Yang Sida Zhou Xinan Zhou Xiaoyu Yan Xinhua Liu | 2021 | Automotive Innovation2021，4，4： | 1 |
| 6 | Exosomes engineered to deliver microRNA-21-5p promote DRG neuron growth in vitro by targeting regeneration-related genes显示文摘The repairment after peripheral nerve injury is a very important field in medical research.Exosomes(Exo)is a natural nano-scale carrier for drugs.They can mediate the transfection of RNA and other signal molecules into cells.Therefore,Exo can be used to bring the genetic materials into target cells,and regulate the expression of related genes.In this study,microRNA-21-5p(miR-21-5p)was introduced into BMSCs-derived Exo.After transfecting into DRG neurons,we uncovered their effects on stimulating the growth of DRG neurons and explored possible mechanis.The exosomes engineered with miR-21-5p/miR-NC were prepared by electroporation to obtain iExosomes-21/iExosomes-NC.DRG neurons of SD rats were cultured in vitro. | He Yuebin Xiao Le Cui Tianwei Ni Panli Yi Xinan | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 7 | High efficient broad-spectrum Bradyrhizobium elkanii Y63-1显示文摘Soybean(Glycine max),the primary source of high-quality plant protein,plays a crucial role as a grain and oil crop in China.Harnessing the full potential of symbiotic nitrogen fixation in soybean production holds immense significance for agriculture and ecology alike.Zhongdou 63,a newly developed early-maturing summer soybean cultivar in 2021,exhibits remarkable traits such as high yield,superior quality,multi-resistance,and wide adaptability.In this study,eight distinct rhizobia strains from diverse regions were meticulously screened to identify highly effective strains specifically suited for Zhongdou 63.The aboveground biomass,plant height,chlorophyll content,root length,nodule number,and nodule dry weight of Zhongdou 63 were measured and the data were subjected to statistical analysis.The results demonstrated that Y63-1 is a predominant strain of Zhongdou 63.Subsequently,we conducted further investigations on the broad-spectrum nodulation characteristics of Y63-1.Ten representative soybean cultivars were individually inoculated with Y63-1 and subsequently analyzed for nodule numbers and nodule dry weight in their symbiotic systems with rhizobia.The findings revealed that Y63-1 effectively formed nodules with all ten soybean varieties tested.In summary,our current study identified highly efficient broad-spectrum Bradyrhizobium elkanii strain Y63-1 as the predominant strain in Zhongdou 63 and provided a theoretical foundation for enhancing yield potential not only in Zhongdou 63 but also in other varieties through inoculation with highly efficient rhizobia in production. | Piao Leng Fuxiao Jin Song Li Yi Huang Chanjuan Zhang Zhihui Shan Zhonglu Yang Limiao Chen Dong Cao Qingnan Hao Wei Guo Hongli Yang Shuilian Chen Xinan Zhou Songli Yuan Haifeng Chen | 2023 | Oil Crop Science2023，8，4： | 0 |
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