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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Effects of dietary supplementation of Artemisia argyi aqueous extract on antioxidant indexes of small intestine in broilers显示文摘The present study was conducted to investigate the effect of Artemisia argyi aqueous extract(AAE) on antioxidant indexes in the small intestine. A total of 192 Arbor Acre broiler chickens(one-day-old) were randomly divided into 4 treatments with 6 replicates of 8 chickens. These 4 diets were formulated by adding 0, 500,1,000 and 2,000 mg/kg AAE to the basal diet. The results showed as follows: 1) compared with the control, the total antioxidant capacity(T-AOC) in ileum for the 2,000 mg/kg treatment group was significantly increased at 21 days of age(P < 0.05); the T-AOC levels in jejunum and ileum were significantly increased in broilers supplemented with 500 mg/kg AAE at 42 days of age(P < 0.05), and the T-AOC levels in jejunum and ileum were significantly improved in 1,000 mg/kg treatment group(P < 0.01), 2) At 21 days of age, supplementation of 500 mg/kg AAE significantly increased the catalase(CAT) activity of small intestine, and the glutathione peroxidase(GSH-Px) activity of jejunum was improved(P < 0.01), meanwhile, the GSH-Px activity of duodenum and the total superoxide dismutase(T-SOD) activity of duodenum and jejunum were significantly higher than those of the control group(P < 0.05); supplementation of 1,000 mg/kg AAE significantly increased the CAT activity of duodenum and ileum and the GSH-Px activity of duodenum and jejunum(P < 0.05), and the ileum GSH-Px activity was significantly increased(P < 0.01); supplementation of 2,000 mg/kg AAE significantly increased the CAT activity of duodenum and ileum(P < 0.05). At 42 days of age, supplementation of 500 mg/kg AAE significantly increased the GSH-Px activity of ileum and the T-SOD activity of duodenum(P < 0.05),meanwhile, the T-SOD activity of jejunum was significantly increased(P < 0.01); supplementation of1,000 mg/kg AAE significantly increased the CAT activity of jejunum and the T-SOD activity of ileum(P < 0.01), and the GSH-Px activity of jejunum was significantly increased(P < 0.05); supplementation of2,000 mg/kg AAE significantly increased the T-SOD activity of ileum(P < 0.05), but significantly decreased the CAT activity of ileum and the GSH-Px activity of jejunum(P < 0.05). 3) The malondialdehyde(MDA) levels of 3 AAE supplementation groups were significantly decreased at 21 and 42 days of age(P < 0.05). The results suggested that dietary supplementation with AAE could improve the antioxidative capacity of small intestine in broilers. | Fei Zhao Binlin Shi Dengsheng Sun Hongyan Chen Manman Tong Pengfei Zhang Xiaoyu Guo Sumei Yan | 2016 | Animal Nutrition2016，，3： | 18 |
| 3 | Chemotherapy-induced intestinal inflammatory responses are mediated by exosome secretion of double-strand DNA via AIM2 inflammasome activation显示文摘化疗经常被知道导致严重的胃肠的道毒性,但是内在的机制仍然保持不清楚。这研究作为一个代表性的代理人认为广泛地应用的细胞毒素的代理人是 irinotecan (CPT-11 ) 并且证明治疗从说明混合物的限制剂量的肠的毒性的肠导致双海滨 DNA 的巨大的版本。明确地,通过 exosome 分泌物释放的 self-DNA 进入天生的有免疫力的房间的 cytosol 并且激活 AIM2 (在黑瘤 2 不在) inflammasome。这导致成熟 IL-1 和 IL-18 分泌物并且导致肠的 mucositis 和迟了发作的腹泻。有趣地,在 AIM2 缺乏的老鼠或由象镇静药那样的一个药理学禁止者,没有影响 CPT-11 的 anticancer 功效, AIM2 发信号的废除显著地减少导致药的腹泻的发生。这些调查结果提供化疗怎么触发引起肠的毒性的天生的有免疫力的回答的机械学的卓见,并且揭示维持反瘤效果,但是围绕联系不利煽动性的反应的新化疗政体。 | Lian, Qiaoshi Xu, Jun Yan, Shanshan Huang, Min Ding, Honghua Sun, Xiaoyu Bi, Aiwei Ding, Jian Sun, Bing Geng, Meiyu | 2017 | Cell Research2017，27，6： | 18 |
| 4 | Cloning of a gene-edited macaque monkey by somatic cell nuclear transfer显示文摘Cloning of macaque monkeys by somatic cell nucleus transfer(SCNT) allows the generation of monkeys with uniform genetic backgrounds that are useful for the development of non-human primate models of human diseases. Here, we report the feasibility of this approach by SCNT of fibroblasts from a macaque monkey(Macaca fascicularis), in which a core circadian transcription factor BMAL1 was knocked out by clustered regularly interspaced short palindromic repeat/Cas9 gene editing(see accompanying paper).Out of 325 SCNT embryos transferred into 65 surrogate monkeys, we cloned five macaque monkeys with BMAL1 mutations in both alleles without mosaicism, with nuclear genes identical to that of the fibroblast donor monkey. Further peripheral blood m RNA analysis confirmed the complete absence of the wild-type BMAL1 transcript. This study demonstrates that the SCNT approach could be used to generate cloned monkeys from fibroblasts of a young adult monkeys and paves the way for the development of macaque monkey disease models with uniform genetic backgrounds. | Zhen Liu Yijun Cai Zhaodi Liao Yuting Xu Yan Wang Zhanyang Wang Xiaoyu Jiang Yuzhuo Li Yong Lu Yanhong Nie Xiaotong Zhang Chunyang Li Xinyan Bian Mu-ming Poo Hung-Chun Chang Qiang Sun | 2019 | National Science Review2019，6，1： | 14 |
| 5 | Timing and splitting of nitrogen fertilizer supply to increase crop yield and efficiency of nitrogen utilization in a wheat–peanut relay intercropping system in China显示文摘Agronomically optimizing the timing and rates of nitrogen(N) fertilizer application can increase crop yield and decrease N loss to the environment. Wheat(Triticum aestivum L.)–peanut(Arachis hypogaea L.) relay intercropping systems are a mainstay of economic and food security in China. We performed a field experiment to investigate the effects of N fertilizer on N recovery efficiency, crop yield, and N loss rate in wheat–peanut relay intercropping systems in the Huang-Huai-Hai Plain, China during 2015–2017. The N was applied on the day before sowing, the jointing stage(G30) or the booting stage(G40) of winter wheat, and the anthesis stage(R1) of peanut in the following percentage splits: 50-50-0-0(N1), 35-35-0-30(N2), and 35-0-35-30(N3), using 300 kg N ha-1, with 0 kg N ha-1(N0) as control. ^(15)N-labeled(20.14 atom %) urea was used to trace the fate of N in microplots. The yields of wheat and peanut increased by 12.4% and 15.4% under the N2 and N3 treatments, relative to those under the N1 treatment. The ^(15)N recovery efficiencies( ^(15)NRE) were 64.9% and 58.1% for treatments N2 and N3, significantly greater than that for the N1 treatment(45.3%). The potential N loss rates for the treatments N2 and N3 were23.7% and 7.0%, significantly lower than that for treatment N1(30.1%). Withholding N supply until the booting stage(N3) did not reduce the wheat grain yield; however, it increased the N content derived from ^(15)N-labeled urea in peanuts, promoted the distribution of ^(15)N to pods, and ultimately increased pod yields in comparison with those obtained by topdressing N at jointing stage(N2). In comparison with N2, the N uptake and N recovery efficiency(NRE) of N3 was increased by 12.0% and 24.1%,respectively, while the apparent N loss decreased by 16.7%. In conclusion, applying N fertilizer with three splits and delaying topdressing fertilization until G40 of winter wheat increased total grain yields and NRE and reduced N loss. This practice could be an environment-friendly N management strategy for wheat–peanut relay intercropping systems in China. | Zhaoxin Liu Fang Gao Yan Liu Jianqun Yang Xiaoyu Zhen Xinxin Li Ying Li Jihao Zhao Jinrong Li Bichang Qian Dongqing Yang Xiangdong Li | 2019 | The Crop Journal2019，7，1： | 12 |
| 6 | The in vitro effect of lipopolysaccharide on proliferation, inflammatory factors and antioxidant enzyme activity in bovine mammary epithelial cells显示文摘Lipopolysaccharide(LPS) was selected as a stimulus to investigate its effect on cell viability and oxidative stress in bovine mammary epithelial cells(BMEC) by detecting the cell relative growth rate(RGR),antioxidant indicators and inflammatory factors. This information was used to provide the theoretical basis for the establishment of a LPS-induced oxidative damage model. The experiment was divided into two parts. The first part used a two-factor experimental design to determine the appropriate incubation time of LPS by detecting the RGR. The third-passage BMEC were divided into 24 groups with six replicates in each group. The first factor was LPS concentration, which was 0(control), 0.1,1.0 and 10.0 μg/mL;the second factor was LPS incubation time(2,4, 6, 8,12 and 24 h). The optimum LPS incubation time was6 h according to the results of the first part of the experiment. The second part of the experiment was conducted using a single-factor experimental design, and the third-passage cells were divided into four groups with six replicates in each group. The cells were incubated with culture medium containing different concentrations of LPS(0 [control], 0.1, 1.0 and 10.0 μg/mL) for 6 h to select the appropriate concentration of LPS to measure the antioxidant indicators and inflammatory factors. The results showed the RGR was significantly reduced as the concentration of LPS and the incubation time increased;the interaction between concentration and incubation time was also significant. The cells treated with0.1 μg/mL of LPS for 6 h had no significant difference in the activities of glutathione peroxidase(GPx) and superoxide dismutase(SOD)(P > 0.05) compared with the cells in the control group. On the contrary,catalase(CAT) activity and malondialdehyde(MDA) content were markedly lower and higher, respectively, in the 0.1 μg/mL LPS-treated group for 6 h compared with the control group(P < 0.05). The activities of GPx, CAT and SOD in the BMEC treated with 1.0 or 10.0 μg/mL of LPS were significantly lower compared with the cells treated with 0.1 μg/mL of LPS and cells in the control group after 6 h of incubation; however, the opposite trend was detected in MDA content. There was no significant(P > 0.05)difference between the 10.0 and 1.0 μg/mL LPS-treated groups. Compared with the control group,interleukin-1, interleukin-6 and nitric oxide concentrations and the activity of inducible nitric oxide synthase in the 0.1 μg/mL LPS-treated group significantly increased(P < 0.0001), but the levels of tumour necrosis factor did not significantly change(P > 0.05). All of observed indicators were higher in the 1.0 and 10.0 μg/mL LPS-treated groups(P < 0.0001) compared with the other groups, but there was no significant(P> 0.05) difference between the 1.0 and 10.0 μg/mL LPS-treated groups. The results indicated that a concentration of 1.0 μg/mL of LPS and an incubation time of 6 h were the optimum conditions necessary to induce oxidative stress in the BMEC and establish a model for oxidative damage. | Huiyu Shi Yongmei Guo Yang Liu Binlin Shi Xiaoyu Guo Lu Jin Sumei Yan | 2016 | Animal Nutrition2016，，2： | 11 |
| 7 | Structural basis for the impact of phosphorylation on the activation of plant receptor-like kinase BAK1显示文摘 | Liming Yan Yuanyuan Ma Dan Liu Xiaochao Wei Yuna Sun Xiaoyue Chen Huadong Zhao Jingwen Zhou Zhiyong Wang Wenqing Shui Zhiyong Lou | 2012 | Cell Research2012，22，8： | 10 |
| 8 | Glycine attenuates myocardial ischemia-reperfusion injury by inhibiting myocardial apoptosis in rats显示文摘Glycine is a well-documented cytoprotective agent.However,whether it has a protective effect against myocar-dial ischemia-reperfusion injury in vivo is still unknown.By using an open-chest anesthetized rat model,we found that glycine reduced the infarct size by 21% in ischemia-reperfusion injury rats compared with that in the vehicle-treated MI/R rats.The left ventricular ejection fraction and fractional shortening were increased by 19.11% and 30.98%,respectively,in glycine-treated rats.The plasma creatine kinase levels in ischemia-reperfusion injury rats decreased following glycine treatment.Importantly,administration of glycine significantly inhibited apoptosis in post-ischemia-reperfusion myocardium,which was accompanied by suppression of phosphorylated p38 mitogen-activated protein kinase and c-Jun NH2-terminal kinase,as well as the Fas ligand.These results suggest that gly-cine attenuates myocardial ischemia-reperfusion injury in vivo by inhibiting cardiomyocytes apoptosis. | Xiaozheng Zhong Xiaoyu Li Lingling Qiana Yiming Xu Yan Lu Jing Zhang Nan Li Xudong Zhu Jingjing Ben Qing Yang Qi Chen | 2012 | The Journal of Biomedical Research2012，26，5： | 10 |
| 9 | Class A scavenger receptor activation inhibits endoplasmic reticulum stress-induced autophagy in macrophage显示文摘Macrophage death in advanced atherosclerosis promotes plaque necrosis and destabilization.Involvement of autophagy in bulk degradation of cellular components has been recognized recently as an important mechanism for cell survival under endoplasmic reticulum(ER) stress.We previously found that the engagement of class A scavenger receptor(SR-A) triggered JNK-dependent apoptosis in ER-stressed macrophages.However,pro-apoptotic mechanisms mediated by SR-A are not fully understood.Therefore,we sought to see if SR-A mediated apoptosis was associated with autophagy in macrophages.Here,we showed that fucoidan inhibited microtubule-associated protein light chain 3-phospholipid conjugates(LC3-Ⅱ) formation as well as the number of autophagosomes under ER stress.The inhibition of LC3-Ⅱ formation was paralleled by the activation of the mTOR pathway,and the inhibition of mTOR allowed LC3-Ⅱ induction in macrophages treated with thapsigargin plus fucoidan.Furthermore,apoptosis induced by fucoidan was prevented under ER stress by the mTOR inhibitor.We propose that fucoidan,a SR-A agonist,may contribute to macrophage apoptosis during ER stress by inhibiting autophagy. | Hanpeng Huang Xiaoyu Li Yan Zhuang Nan Li Xudong Zhu Jin Hu Jingjing Ben Qing Yang Hui Bai Qi Chen | 2014 | The Journal of Biomedical Research2014，28，3： | 9 |
| 10 | Up-regulation of hypoxia-inducible factor-1α enhanced the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats显示文摘Hyperlipidemia 是在 ischemic 心疾病的发展的一个独立风险因素,它能增加心肌的危险性到 ischemia/reperfusion (I/R ) 损害。Ischemic postconditioning (PostC ) 现在作为新奇策略被表明了处于正常条件对心肌的 I/R 损害利用自然的保护。然而, hyperlipidemic 动物上的 PostC 的效果留下逃犯。PostC 减少心肌的 I/R 损害,这在我们的以前的学习被显示出,并且组织缺氧可诱导的 factor-1 (HIF-1 ) 可以在正常老鼠上在 PostC 的 cardioprotective 机制起一个重要作用。这里,我们测试了 hyperlipidemic 老鼠上的 PostC 的 cardioprotection 与起来调整的 HIF-1 表示被联系的假设。男 Wistar 老鼠用一本高脂肪的食谱被喂 8 个星期,然后随机把组划分了成五:假冒, I/R, dimethyloxalylglycine (DMOG )+ I/R, PostC,和 DMOG + PostC 组织。I/R 损害导致的有害索引包括了梗塞尺寸,血浆肌酸 kinase (CK ) 活动和 caspase-3 活动。,结果与 I/R 组相比证明 PostC 能减少梗塞尺寸,它与血浆 CK 的重要底层一致活动和 caspase-3 活动,和那它在 hyperlipidemic 老鼠增加了 HIF-1 的表示。当 DMOG 在 PostC 前被给起来调整 HIF-1 蛋白质水平时, I/R 损害的度被稀释。在结论,这些数据建议 HIF-1 的起来规定可以是对在 hyperlipidemic 老鼠的 I/R 损害的 PostC 的 cardioprotective 机制之一。 | Xiaoyu Li Huanxin Zhao Ye Wu Suli Zhang Xiaoqin Zhao Yan Zhang Jin Wang Jie Wang Huirong Liu | 2014 | Acta Biochimica et Biophysica Sinica2014，46，2： | 9 |
| 11 | Enhanced methane production in an anaerobic digestion and microbial electrolysis cell coupled system with co-cultivation of Geobacter and Methanosarcina显示文摘The anaerobic digestion(AD)and microbial electrolysis cell(MEC)coupled system has been proved to be a promising process for biomethane production.In this paper,it was found that by co-cultivating Geobacter with Methanosarcina in an AD–MEC coupled system,methane yield was further increased by 24.1%,achieving to 360.2 m L/g-COD,which was comparable to the theoretical methane yield of an anaerobic digester.With the presence of Geobacter,the maximum chemical oxygen demand(COD)removal rate(216.8 mg COD/(L·hr))and current density(304.3 A/m_3)were both increased by 1.3 and 1.8 fold compared to the previous study without Geobacter,resulting in overall energy efficiency reaching up to 74.6%.Community analysis demonstrated that Geobacter and Methanosarcina could coexist together in the biofilm,and the electrochemical activities of both were confirmed by cyclic voltammetry.Our study observed that the carbon dioxide content in total gas generated from the AD reactor with Geobacter was only half of that generated from the same reactor without Geobacter,suggesting that Methanosarcina may obtain the electron transferred from Geobacter for the reduction of carbon dioxide to methane.Taken together,Geobacter not only can improve the performance of the MEC system,but also can enhance methane production. | Qi Yin Xiaoyu Zhu Guoqiang Zhan Tao Bo Yanfei Yang Yong Tao Xiaohong He Daping Li Zhiying Yan | 2016 | Journal of Environmental Sciences2016，28，4： | 9 |
| 12 | A spatial-explicit dynamic vegetation model that couples carbon,water,and nitrogen processes for arid and semiarid ecosystems显示文摘Arid and semiarid ecosystems,or dryland,are important to global biogeochemical cycles.Dryland's community structure and vegetation dynamics as well as biogeochemical cycles are sensitive to changes in climate and atmospheric composition.Vegetation dynamic models has been applied in global change studies,but the complex interactions among the carbon(C),water,and nitrogen(N) cycles have not been adequately addressed in the current models.In this study,a process-based vegetation dynamic model was developed to study the responses of dryland ecosystems to environmental changes,emphasizing on the interactions among the C,water,and N processes.To address the interactions between the C and water processes,it not only considers the effects of annual precipitation on vegetation distribution and soil moisture on organic matter(SOM) decomposition,but also explicitly models root competition for water and the water compensation processes.To address the interactions between C and N processes,it models the soil inorganic mater processes,such as N mineralization/immobilization,denitrification/nitrification,and N leaching,as well as the root competition for soil N.The model was parameterized for major plant functional types and evaluated against field observations. | Chi ZHANG ChaoFan LI Xi CHEN GePing LUO LongHui LI XiaoYu LI Yan YAN Hua SHAO | 2013 | Journal of Arid Land2013，5，1： | 8 |
| 13 | OsCIPK7 point-mutation leads to conformation and kinase-activity change for sensing cold response显示文摘Calcineurin B-like interacting protein kinases(CIPKs) play important roles via environmental stress.However, less is known how to sense the stress in molecular structure conformation level. Here, an Os CIPK7 mutant via TILLING procedure with a point mutation in the kinase domain showed increased chilling tolerance, which could be potentially used in the molecular breeding. We found that this point mutation of Os CIPK7 led to a conformationalchange in the activation loop of the kinase domain,subsequently with an increase of protein kinase activity,thus conferred an increased tolerance to chilling stress. | Dajian Zhang Xiaoyu Guo Yunyuan Xu Hao Li Liang Ma Xuefeng Yao Yuxiang Weng Yan Guo Chun-Ming Liu Kang Chong | 2019 | Journal of Integrative Plant Biology2019，61，12： | 8 |
| 14 | Genesis of the low-permeability reservoir bed of upper Triassic Xujiahe Formation in Xinchang gas field,western Sichuan Depression显示文摘一座水库的开始是免职的联合行动的结果,成岩作用,构造重做,并且岩石和液体和进化环境的相互作用。我们讨论基因并且在西方的四川消沉的 Xujiahe 形成的一张低渗透的水库床的进化机制根据成岩作用, diagenetic 水库外形和 diagenetic 进化顺序的学习。研究显示这张水库床能被划分成答案,碳酸盐硬化和第二等的石英增加的 diagenetic 水库外形,也就是强壮的溶解,排队绿泥石的 intergranular 毛孔,压缩和压力的五种类型。然而,只有二 diagenetic 水库外形,提供低渗透的水库床,也就是强壮的溶解和排队绿泥石的 intergranular 毛孔。我们也分析了他们的 diagenetic 进化序列和低渗透的水库床的起源。而且,沙岩,沉积 microfacies 的类型,成岩作用历史以及在以后的时期的构造重做的作文和结构是控制低渗透的水库床的形成的主要因素,这也被显示。上述的因素为预报建立基础高质量的水库床的分发。 | Xu Zhangyou Zhang Xiaoyu Wu Shenghe Zhao Yan | 2008 | Petroleum Science2008，5，3： | 8 |
| 15 | Mitochondrial replacement by pre-pronuclear transfer in human embryos显示文摘 | Wu, Keliang Chen, Tailai Huang, Sexin Zhong, Cuiqing Yan, Junhao Zhang, Xiaoyu Li, Jinsong Gao, Yuan Zhao, Han Chen, Zi-Jiang | 2017 | Cell Research2017，27，6： | 7 |
| 16 | Hypertensive stretch regulates endothelial exocytosis of WeibeI-Palade bodies through VEGF receptor 2 signaling pathways显示文摘Weibel-Palade 身体(WPB ) 的调整 endothelial exocytosis,在白血球 trafficking 的第一个阶段,在发炎和损害起一个枢轴的作用。尖锐的机械段仔细与脉管的发炎被联系了,尽管精确机制是未知的。这里,我们证明那高血压的段通过 VEGF 受体 2 调整 endothelial 房间(EC ) 的 WPB 的 exocytosis (VEGFR2 ) 表明小径。段触发一个快速的版本(在分钟以内) 从在有教养的人的 EC 的 WPB 的 von Willebrand 因素和 interleukin-8,通过 P-selectin 的 translocation 把在白血球和 EC 之间的相互作用提升到房间膜。我们进一步证明那高血压的段显著地导致未经触动的 EC 的 P-selectin translocation 并且两个都提高白血球粘附前 vivo 并且在 vivo。导致段的 endothelial exocytosis 经由 VEGFR2/PLCγ 被调停; 1/calcium 小径。有趣地,段也经由一条 VEGFR2/Akt/nitric 氧化物小径导致否定反馈。如此的双效果在颈动脉动脉片断,以及在尖锐高血压的老鼠模型用药理学和基因途径被证实。这些研究为 endothelial exocytosis 作为有势力收缩筋揭示机械段,它被 VEGFR2 发信号调制。因此, VEGFR2 发信号小径可以在限制高血压的段相关的发炎代表新奇治疗学的目标。 | Yan Xiong Zhenqian Hu Xiaofan Han Beibei Jiang Rongli Zhan Xiaoyu Zhang Yao Lu Chenyang Geng Wei Li Yulong He Yingqing Huo Masabuml Shlbuya Jincai Luo | 2013 | Cell Research2013，23，6： | 7 |
| 17 | A draft sequence of the rice (Oryza sativa ssp. indica) genome显示文摘The sequence of the rice genome holds fundamental information for its biology, including physiology, genetics, development, and evolution, as well as information on many beneficial phenotypes of economic significance. Using a 'whole genome shotgun' approach, we have pro-duced a draft rice genome sequence of Oryza sativa ssp. in-dica, the major crop rice subspecies in China and many other regions of Asia. The draft genome sequence is constructed from over 4.3 million successful sequencing traces with an accumulative total length of 2214.9 Mb. The initial assembly of the non-redundant sequences reached 409.76 Mb in length, based on 3.30 million successful sequencing traces with a total length of 1797.4 Mb from an indica variant cultivar 93-11, giving an estimated coverage of 95.29% of the rice genome with an average base accuracy of higher than 99%. The coverage of the draft sequence, the randomness of the sequence distribution, and the consistency of BIG-ASSEM-BLER, a custom-designed software package | YU Jun, HU Songnian, WANG Jun,LI Songgang WONG Ka-Shu Gane, LIU Bin,DENG Yajun, DAI Li, ZHOU Yan,ZHANG Xiuqing, CAO Mengliang, LIU Jing,SUN Jiandong , TANG Jiabin, CHEN Yanjiong,HUANG Xiaobing, LIN Wei, YE Chen, TONG Wei,CONG Lijuan, GENG Jianing, HAN Yujun, LI Lin,LI Wei, HU Guangqiang, HUANG Xiangang,LI Wenjie, LI Jian, LIU Zhanwei, LI Long,LIU Jianping, Ql Qiuhui, LIU Jinsong, LI Li,WANG Xuegang, LU Hong, WU Tingling,ZHU Miao, Nl Peixiang, HAN Hua, DONG Wei,REN Xiaoyu, FENG Xiaoli, GUI Peng,LI Xianran, WANG Hao, XU Xin, ZHAI Wenxue,XU Zhao, ZHANG Jinsong, HE Sijie,ZHANG Jianguo, XU Jichen, ZHANG Kunlin,ZHENG Xianwu, DONG Jianhai, ZENG Wanyong,TAO Lin, CHEN Xuewei, HE Jun, LIU Daofeng,TIAN Wei, TIAN Chaoguang, XIA Hongai,LI Gang, GAO Hui, LI Ping, CHEN Wei ,WANG Xudong, ZHANG Yong, HU Jianfei,WANG Jing, LIU Song, YANG Jian,ZHANG Guangyu, XIONG Yuqing, LI Zhijie,MAO Long, ZHOU Chengshu, ZHU Zhen,CHEN Runsheng, HAO Bailin,ZHENG Weimou, CHEN Shouyi, QUO Wei,LI Guojie, LIU Siqi, HUANG Guyang,TAO Ming, WANG Jian, ZHU Lihuang,YUAN Longping& YANG HuanmingBeijing Genomics Institute/Center of Genomics & Bioinformatics, Chinese Academy of Sciences, Beijing 101300, China Hangzhou Genomics Institute/Institute of Bioinformatics of Zhejiang University/Key Laboratory of Bioinformatics of Zhejiang Province, Hangzhou 310007, China Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China National Hybrid Rice R & D Center, Changsha 410125, China Laboratory of Bioinformatics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China College of Life Sciences, Peking University, Beijing 100871, China Institute of Theoretical Physics, Chinese Academy of Sciences, Beijing 1Q0080, China Digital China Ltd., Beijing 100080, China Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100080, China Medical College, Xi’an Jiaotong University, Xi’an 710061, ChinaThese authors contributed equally to this work.Corresponding author.Corresponden | 2001 | Chinese Science Bulletin2001，46，23： | 6 |
| 18 | Lithium chloride promotes osteogenesis and suppresses apoptosis during orthodontic tooth movement in osteoporotic model via regulating autophagy显示文摘Osteoporosis is a widely distributed disease that may cause complications such as accelerated tooth movement,bone resorption,and tooth loss during orthodontic treatment.Promoting bone formation and reducing bone resorption are strategies for controlling these complications.For several decades,the autophagy inducer lithium chloride(LiCl)has been explored for bipolar.In this study,we investigated the autophagy-promoting effect of LiCl on bone remodeling under osteoporotic conditions during tooth movement.Ovariectomy was used to induce osteoporosis status in vivo.The results showed that LiCl rejuvenated autophagy,decreased apoptosis,and promoted bone formation,thus protecting tooth movement in osteoporotic mice.Furthermore,in vitro experiments showed that LiCl reversed the effects of ovariectomy on bone marrow-derived mesenchymal stem cells(BMSCs)extracted from ovariectomized mice,promoting osteogenesis and suppressing apoptosis by positively regulating autophagy.These findings suggest that LiCl can significantly decrease adverse effects of osteoporosis on bone remodeling,and that it has great potential significance in the field of bone formation during tooth movement in osteoporosis patients. | Li Huang Xing Yin Jun Chen Ruojing Liu Xiaoyue Xiao Zhiai Hu Yan He Shujuan Zou | 2021 | Bioactive Materials2021，6，10： | 6 |
| 19 | Electrical stimulation of the sacral nerve anterior root following induced bladder detrusor contraction显示文摘The sacral nerve anterior root consists of parasympathetic nerves(dominating urinary bladder detrusor) and somatic motor nerves(dominating urethral sphincter),and electrical stimulation to the sacral nerve anterior root induces simultaneous contraction of the bladder detrusor and urethral sphincter. Accordingly,urethral pressure exceeds intravesical pressure,resulting in little or no urination,kidney damage,and trembling of lower limbs due to high intravesical pressure. In the present study,sacral nerve posterior roots were transected in a spastic bladder rabbit model,followed by three-pole electrode and long-pulse electrical stimulation to the sacral anterior root. Intravesical and urethral pressures were simultaneously measured to verify the feasibility of anode inhibition to the sacral anterior root following induced detrusor contraction. As stimulus intensity increased,somatic motor nerves were increasingly inhibited;with a stimulus pulse width of 300 μs and stimulus current of 1.05 mA,urethral pressure was zero and average intravesical pressure was 3.84 kPa. In addition,detrusor contraction was displayed,and lower extremity trembling was significantly reduced. Three-pole electrode and long-pulse electrical stimulation to the sacral nerve anterior root induced detrusor contraction and inhibited low extremity trembling under electrical stimulation. | Peng Yan Xiaoyu Yang Qi Gao Xiaoran Wang Jian Liu Maoguang Yang | 2010 | Neural Regeneration Research2010，5，24： | 6 |
| 20 | In vitro testicular organogenesis from human fetal gonads produces fertilization-competent spermatids显示文摘Unlike most organs that mature during the fetal period,the male reproductive system reaches maturity only at puberty with the commencement of spermatogenesis.Robust modelling of human testicular organogenesis in vitro would facilitate research into mechanisms of and factors affecting human spermatogenic failure and male fertility preservation in prepubertal tumor patients.Here,we report successful recapitulation of human testicular organogenesis in vitro from fetal gonadal ridge.Our model displayed the formation of mature seminiferous epithelium and self-renewing spermatogonia.Remarkably,in vitro-derived haploid spermatids have undergone meiotic recombination,and showed increased genetic diversity as indicated by genetic analysis.Moreover,these spermatids were able to fertilize oocytes and support subsequent blastocyst formation.The in vitro testicular organogenesis system described here will play an important role in elucidating the regulation of human testis development and maintaining male fertility in prepubertal cancer patients. | Yan Yuan Laihua Li Qing Cheng Feiyang Diao Qiao Zeng Xiaoyu Yang Yibo Wu Hao Zhang Mingqian Huang Junqing Chen Quan Zhou Yunfei Zhu Rong Hua Jianyu Tian Xin Wang Zuomin Zhou Jie Hao Jinjin Yu Dong Hua Jiayin Liu Xuejiang Guo Qi Zhou Jiahao Sha | 2020 | Cell Research2020，30，3： | 6 |
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