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| 1 | Genome Editing and Double-Fluorescence Proteins Enable Robust Maternal Haploid Induction and Identification in Maize显示文摘 | Le Dong Lina Li Changlin Liu Chenxu Liu Shuaifeng Geng Xinhai Li Changling Huang Long Mao Shaojiang Chen Chuanxiao Xie | 2018 | Molecular Plant2018，11，9： | 22 |
| 2 | Genome Editing Enables Next-Generation Hybrid Seed Production Technology显示文摘The next-generation hybrid seed technology enables the successful production of sortable hybrid seeds from genic male sterile(GMS)lines and maintainers;however,it requires multiple laborious and complicated steps.Here,we designed a simple next-generation hybrid seed production strategy that takes advantage of the CRISPR/Cas9 technology to create a Manipulated GMS Maintainer(MGM)system via a single transformation.Under this schema,the maize male fertility gene ZmMS26 was nullified by removal of its fifth exon using the CRISPR/Cas9 system on a vector,and a second vector carrying a functional ZmMS26 cDNA was co-transformed to restore fertility.The second vector also contains a male gametophyte inactivation gene(ZmAA1)encoding maizeα-amylase driven by the pollen-specific promoter PG47 and an endosperm fluorescent marker(DsRED)driven by the barley endosperm aleurone-specific promoter Ltp2.The derived single-copy hemizygous MGM lines bore a mutated MS26 gene,leading to complete male sterility but normal vegetative growth and grain yield.The MGM system could prevent genetic transmission of the MGM elements via male gametophytes,providing an efficient method for sorting maintainer seeds labeled by DsRED.This strategy can be extended to any GMS gene and to hybrid crops other than maize. | Xiantao Qi Congsheng Zhang Jinjie Zhu Changlin Liu Changling Huang Xinhai Li Chuanxiao Xie | 2020 | Molecular Plant2020，13，9： | 18 |
| 3 | Sensitivity Analysis Based Multiple Objective Preform Die Shape Optimal Design in Metal Forging显示文摘多重客观预先形成设计优化被提出。finalforging 的形状和变丑一致性在多重目的被考虑。目的是优化形状和同时的最后的前进的变丑一致性以便更多高集成前进的期末考试的质量能被获得。全部的目的被形状和一致性目的用增加方法的重量装配。预先形成死形状被立方的 B 花键曲线介绍。B 花键曲线的控制点被用作设计变量。全部的客观功能,形状和一致性 sub-objectivefunction 的形式被给。关于设计变量的全部的客观功能和 sub-objectivefunctions 的敏感被开发。用这个方法,预先形成死一个塑造 H 的前进过程的形状最佳地被设计。优化结果是很令人满意的。 | Xinhai ZHAO Guoqun ZHAO Guangchun WANG Tonghai WANG | 2006 | Journal of Materials Science & Technology2006，22，2： | 10 |
| 4 | Genetic characterization and linkage disequilibrium mapping of resistance to gray leaf spot in maize(Zea mays L.)显示文摘Gray leaf spot(GLS),caused by Cercospora zeae-maydis,is an important foliar disease of maize(Zea mays L.)worldwide,resistance to which is controlled by multiple quantitative trait loci(QTL).To gain insights into the genetic architecture underlying the resistance to this disease,an association mapping population consisting of 161 inbred lines was evaluated for resistance to GLS in a plant pathology nursery at Shenyang in 2010 and 2011.Subsequently,a genome-wide association study,using 41,101 single-nucleotide polymorphisms(SNPs),identified 51 SNPs significantly(P<0.001)associated with GLS resistance,which could be converted into 31 QTL.In addition,three candidate genes related to plant defense were identified,including nucleotidebinding-site/leucine-rich repeat,receptor-like kinase genes similar to those involved in basal defense.Two genic SNPs,PZE-103142893 and PZE-109119001,associated with GLS resistance in chromosome bins 3.07 and 9.07,can be used for marker-assisted selection(MAS)of GLS resistance.These results provide an important resource for developing molecular markers closely linked with the target trait,enhancing breeding efficiency. | Liyu Shi Xiangling Lv Jianfeng Weng Hanyong Zhu Changlin Liu Zhuanfang Hao Yu Zhou Degui Zhang Mingshun Li Xiaoke Ci Xinhai Li Shihuang Zhang | 2014 | The Crop Journal2014，2，Z1： | 9 |
| 5 | Effect of lithium or aluminum substitution on the characteristics of graphite for anode of lithium ion batteries显示文摘Modification of graphite for anode of lithium ion batteries is investigated. Results of X-ray diffraction shows lithium and aluminum exists as Li compound (CH3COOLi@2H2O) and Al compound (AlD3) in the graphite, respectiovely.The Bmnauer-Emmer-Teller (BET) surface area of the modified graphite increases. According to the electrochemical measurements of Li/C cell and prototype Li-ion batteries, the Li-doped graphite has large reversible capacity of 312.2mA@h/g, low irreversible capacity of 52.9 mA@h/g, and high initial coulombic efficiency of 85.51%. The 063448 size prototype battery with Li-doped graphite anode has large discharge capacity of 845 mA@h and good cycling performance. The initial charge/discharge characteristic of Al-doped graphite is close to those of undoped graphite, but the prototype battery with Al-doped anode shows the best cycling performance with capacity retention ratio of 94.06% at the 200th cycle. | GUO Huajun, LI Xinhai, WANG Zhixing, PENG Wenjie, and GUO YongxingCollege of Metallurgical Science and Engineering, Central South University, Changsha 410083, China | 2003 | Rare Metals2003，22，4： | 9 |
| 6 | Evaluation of maize inbred lines currently used in Chinese breeding programs for resistance to six foliar diseases显示文摘Foliar diseases are common in most maize-producing regions and have caused serious yield reduction in China. To evaluate genetic resistance of parental lines actively used in maize breeding programs to major foliar diseases, 152 maize inbred lines were tested against northern corn leaf blight(NCLB), southern corn leaf blight(SCLB), Curvularia leaf spot(CLS),gray leaf spot(GLS), common rust, and southern rust from 2003 to 2005. A small number of lines exhibited highly resistant reactions to common rust and southern rust, but none were highly resistant to NCLB, SCLB, CLS, and GLS. Although 53.3%, 40.8%, and 80.7% of lines were resistant to NCLB, SCLB, and common rust, the resistance in most lines was moderate.Resistance to CLS, GLS, and southern rust was rare in this collection of maize lines. Five lines,313, Chang 7-2, Qi 319, Qi 318, and Shen 137, were resistant to five diseases tested. Lines belonging to heterotic subgroup PB exhibited better resistance to the foliar diseases than lines from other heterotic subgroups, such as BSSS, PA, Lancaster, LRC, and PA. The results will be of benefit to breeders for selecting lines in disease resistance breeding programs. | Xiaoming Wang Yunhua Zhang Xiude Xu Hongjie Li Xiaofei Wu Shihuang Zhang Xinhai Li | 2014 | The Crop Journal2014，2，4： | 8 |
| 7 | Performance and capacity fading reason of LiMn_2O_4/graphite batteries after storing at high temperature显示文摘Spinel LiMn2O4 was synthesized by a solid-state method. A 204468-size battery was fabricated and stored at 55°C. The structure and morphology of the LiMn2O4 cathode were analyzed by X-ray diffraction (XRD) and scanning electron microscopy (SEM) technique. Energy dispersive spectroscopy (EDS) was used to analyze the surface component of the carbon anode. The discharge capacities of LiMn2O4 stored for 0, 24, 48, and 96 h are 106, 98, 96, and 92 mAh?g?1, respectively. The cyclic performance is improved after storage. The capacity retentions of LiMn2O4 stored for 0, 24, 48, and 96 h are 83.8%, 85.8%, 86.9%, and 88.6% after 180 cycles. The intensity of all the LiMn2O4 diffraction peaks is weakened. Mn is detected from the carbon electrode when the battery is stored for 96 h. Cyclic voltammograms and electrochemical impedance spectroscopy (EIS) were used to examine the surface state of the electrode after storage. The results show that the resistance and polarization of LiMn2O4/electrolyte is increased after storage, which is responsible for the fading of capacity. | LIU Yunjian LI Xinhai GUO Huajun WANG Zhixing HU Qiyang PENG Wenjie YANG Yong | 2009 | Rare Metals2009，28，4： | 7 |
| 8 | A secret key distribution technique based on semiconductor superlattice chaos devices显示文摘Key distribution is one of the most important and often also the most difficult parts in cryptography[1].In symmetric key cryptography,both of the sender and the recipient must share the same secure key for successful communication.Traditionally key distribution is achieved by establishing a separate‘‘secure channel'.The‘‘secure channel'can be a specially-built communication link, | Wei Liu Zhizhen Yin Xiaoming Chen Zhenyun Peng Helun Song Peihua Liu Xinhai Tong Yaohui Zhang | 2018 | Science Bulletin2018，63，16： | 7 |
| 9 | Improving the electrochemical performance of LiMn_2O_4/graphite batteries using LiF additive during fabrication显示文摘LiMn2O4/graphite batteries using LiF additive were fabricated and their electrochemical performance including discharge,cycling and storage performances were tested and compared with LiF-free LiMn2O4/graphite batteries.The LiMn2O4/graphite battery with LiF added shows better capacity (107.5 mAh/g),cycling performance (capacity retention ratio of 93% after 100 cycles),and capacity recovery ratio (98.1%) than the LiF-free battery.The improvement in electrochemical performance of the LiF-added LiMn2O4/graphite battery was due to the fact that LiF can restrain the dissolution of Mn from the spinel LiMn2O4 cathode into the electrolyte,leading to a smaller resistance and polarization. | LIU Yunjian GUO Huajun LI Xinhai WANG Zhixing | 2011 | Rare Metals2011，30，2： | 6 |
| 10 | Zea mays(L.) P1 locus for cob glume color identified as a post-domestication selection target with an effect on temperate maize genomes显示文摘Artificial selection during domestication and post-domestication improvement results in loss of genetic diversity near target loci. However, the genetic locus associated with cob glume color and the nature of the genomic pattern surrounding it was elusive and the selection effect in that region was not clear. An association mapping panel consisting of 283 diverse modern temperate maize elite lines was genotyped by a chip containing over 55,000 evenly distributed SNPs. Ten-fold resequencing at the target region on 40 of the panel lines and 47 tropical lines was also undertaken. A genome-wide association study(GWAS) for cob glume color confirmed the P1 locus, which is located on the short arm of chromosome 1, with a-log10 P value for surrounding SNPs higher than the Bonferroni threshold(α/n, α < 0.001) when a mixed linear model(MLM) was implemented. A total of 26 markers were identified in a 0.78 Mb region surrounding the P1 locus, including 0.73 Mb and 0.05 Mb upstream and downstream of the P1 gene, respectively. A clear linkage disequilibrium(LD) block was found and LD decayed very rapidly with increasing physical distance surrounding the P1 locus. The estimates of π and Tajima's D were significantly(P < 0.001) lower at both ends compared to the locus. Upon comparison of temperate and tropical lines at much finer resolution by resequencing(180-fold finer than chip SNPs), a more structured LD block pattern was found among the 40 resequenced temperate lines. All evidence indicates that the P1 locus in temperate maize has not undergone neutral evolution but has been subjected to artificial selection during post-domestication selection or improvement. The information and analytical results generated in this study provide insights as to how breeding efforts have affected genome evolution in crop plants. | Chuanxiao Xie Jianfeng Weng Wenguo Liu Cheng Zou Zhuanfang Hao Wenxue Li Minshun Li Xiaosen Guo Gengyun Zhang Yunbi Xu Xinhai Li Shihuang Zhang | 2013 | The Crop Journal2013，1，1： | 6 |
| 11 | Creation of targeted inversion mutations in plants using an RNA-guided endonuclease显示文摘Inversions are DNA rearrangements that are essential for plant gene evolution and adaptation to environmental changes. We demonstrate the creation of targeted inversions and previously reported targeted deletion mutations via delivery of a pair of RNA-guided endonucleases(RGENs) of CRISPR/Cas9. The efficiencies of the targeted inversions were2.6% and 2.2% in the Arabidopsis FLOWERING TIME(At FT) and TERMINAL FLOWER 1(At TFL1)loci, respectively. Thus, we successfully established an approach that can potentially be used to introduce targeted DNA inversions of interest for functional studies and crop improvement. | Congsheng Zhang Changlin Liu Jianfeng Weng Beijiu Cheng Fang Liu Xinhai Li Chuanxiao Xie | 2017 | The Crop Journal2017，5，1： | 6 |
| 12 | Bird functional traits affect seed dispersal patterns of China's endangered trees across different disturbed habitats显示文摘Background: The most dominant global threat to natural forests and their biodiversity is land-cover change, which has negative impacts on both species persistence and ecosystem functions. Land-cover change could alter animal behaviour and disrupt seed dispersal mutualisms. However, its effects on the role of bird functional traits in seed dispersal are not well studied.Methods: In the present study, we assessed the contributions of bird functional traits(behavioural traits: food habit, foraging pattern, foraging frequency, and habitat specialisation; morphological traits: weight, body length, wing length, and tail length) to both seed removal patterns and seed dispersal distances of an endangered and native tree species, Chinese yew(Taxus chinensis), in farmland, patchy habitat, and natural habitat, of southeast China.Results: We found that the ability of T. chinensis trees to form seed dispersal mutualisms with local birds varied across the different disturbed habitats. As a consequence of these mutualisms, more seeds were removed by birds from the patchy habitat than from the other two habitats. The number of seeds removed increased with bird foraging frequency. Moreover, the dispersal distance from the three habitats differed, and the longest dispersal distances were observed at both the patchy habitat and the farmland site. Seed dispersal distance increased with bird tail and wing length.Conclusions: Our results highlight the importance of bird functional traits in the seed dispersal patterns of endangered trees across disturbed forest habitats, which should be considered for tree conservation and management. | Ning Li Zheng Wang Xinhai Li Zhaohui Li | 2018 | Avian Research2018，9，2： | 6 |
| 13 | Identification of Functional Genetic Variations Underlying Drought Tolerance in Maize Using SNP Markers显示文摘Single nucleotide polymorphism (SNP) is a common form of genetic variation and popularly exists in maize genome. An Illumina GoldenGate assay with 1 536 SNP markers was used to genotype maize inbred lines and identified the functional genetic variations underlying drought tolerance by association analysis. Across 80 lines, 1 006 polymorphic SNPs (65.5% of the total) in the assay with good call quality were used to estimate the pattern of genetic diversity, population structure, and familial relatedness. The analysis showed the best number of fixed subgroups was six, which was consistent with their original sources and results using only simple sequence repeat markers. Pairwise linkage disequilibrium (LD) and association mapping with phenotypic traits investigated under water-stressed and well-watered regimes showed rapid LD decline within 100-500 kb along the physical distance of each chromosome, and that 29 SNPs were associated with at least two phenotypic traits in one or more environments, which were related to drought-tolerant or drought-responsive genes. These drought-tolerant SNPs could be converted into functional markers and then used for maize improvement by marker-assisted selection. | Zhuanfang Hao Xinhai Li Chuanxiao Xie Jianfeng Weng Mingshun Li Degui Zhang Xiaoling Liang Lingling Liu Sisi Liu Shihuang Zhang | 2011 | Journal of Integrative Plant Biology2011，53，8： | 5 |
| 14 | Cooperation of nitrogen-doping and catalysis to improve the Li-ion storage performance of lignin-based hard carbon显示文摘Hard carbon draws great interests as anode material in lithium ion batteries(LIBs)due to its high theoretical capacity,high rate capability and abundance of its precursors.Herein we firstly synthesize the lignin-melamine resins by grafting melamine onto lignin.Afterwards,nitrogen doped hard carbon is prepared by the pyrolysis of lignin-melamine resins with the aid of catalyst(Ni(NO_3)_2·6H_2O)at 1000°C.Compared with the samples without nitrogen-doping and catalysis,as-prepared nitrogen doped hard carbon exhibits higher reversible capacity(345 mAh g^(–1)at 0.1 A g^(-1)),higher rate capability(145 mAh g^(-1)at 5 A g^(–1))and excellent cycling stability.The superior electrochemical performance is ascribed to the synergistic effect of nitrogen doping,graphitic structure and amorphous structure.Among them,nitrogen doping could create the vacancies around the nitrogen sites,which enhance the reactivity and the electronic conductivity of materials.Additionally,graphitic structure also enhances the electronic conductivity of materials,thus improving the electrochemical performance of hard carbon.It is worthwhile that lignin,renewable and abundant biopolymer,is converted to hard carbon with good electrochemical performance,which realizes the high value utilization of lignin. | Zhewei Yang Huajun Guo Feifei Li Xinhai Li Zhixing Wang Lizhi Cui Jiexi Wang | 2018 | Journal of Energy Chemistry2018，27，5： | 5 |
| 15 | Tunable multiband terahertz metamaterials using a reconfigurable electric split-ring resonator array显示文摘We demonstrate micromachined reconfigurable metamaterials working at multiple frequencies simultaneously in the terahertz range.The proposed metamaterial structures can be structurally reconfigured by employing flexible microelectromechanical system-based cantilevers in the resonators,which are designed to deform out of plane under an external stimulus.The proposed metamaterial structures provide not only multiband resonance frequency operation but also polarization-dependent tunability.Three kinds of metamaterials are investigated as electric split-ring resonator(eSRR)arrays with different positions of the split.By moving the position of the split away from the resonator’s center,the eSRR exhibits anisotropy,with the dipole resonance splitting into two resonances.The dipole–dipole coupling strength can be continuously adjusted,which enables the electromagnetic response to be tailored by adjusting the direct current(DC)voltage between the released cantilevers and the silicon substrate.The observed tunability of the eSRRs is found to be dependent on the polarization of the incident terahertz wave.This polarization-dependent tunability is demonstrated by both experimental measurements and electromagnetic simulations. | Fusheng Ma Yu-Sheng Lin Xinhai Zhang Chengkuo Lee | 2014 | Light(Science & Applications)2014，3，1： | 5 |
| 16 | Angle estimation for bistatic MIMO radar with unknown mutual coupling based on three-way compressive sensing显示文摘The problem of angle estimation for bistatic multipleinput multiple-output radar in the present of unknown mutual coupling(MC) is investigated, and a three-way compressive sensing(TWCS) estimation algorithm is developed. To exploit the inherent multi-dimensional structure of received data, a trilinear tensor model is firstly formulated. Then the de-coupling operation is followed. Thereafter, the high-order singular value decomposition is applied to compress the high dimensional tensor to a much smaller one. The estimation of the compressed direction matrices are linked to the compressed trilinear model, and finally two overcomplete dictionaries are constructed for angle estimation. Also,Cramer-Rao bounds for angle and MC estimation are derived. The proposed TWCS algorithm is effective from the perspective of estimation accuracy as well as the computational complexity, and it can achieve automatically paired angle estimation. Simulation results show that the proposed method has much better estimation accuracy than the existing algorithms in the low signal-to-noise ratio scenario, and its estimation performance is very close to the parallel factor analysis(PARAFAC) algorithm at the high SNR regions. | Xinhai Wang Gong Zhang Fangqing Wen De Ben Wenbo Liu | 2017 | Journal of Systems Engineering and Electronics2017，28，2： | 4 |
| 17 | Modeling and understanding of the frequency dependent HPM upset susceptibility of the CMOS inverter显示文摘In this paper, we interpret and, for the first time, model a frequency dependent high-power microwave(HPM) upset susceptibility. We constructed the analytical models of the total amount of injected charges N inj, the excess carrier density distribution in P-Substrate, and the frequency dependent HPM upset susceptibility level. The models are validated by simulated results and experimental data, respectively. Results reveal that N inj is proportional to fαwhile the exponent α should be adjusted to-0.525. The excess carrier density distribution behaves the frequency dependence as well; the dependence is attributable to the fact that the AC field within the CMOS inverter varies too rapidly for the carriers to follow at high frequency. Meanwhile,the HPM upset susceptibility level is a decreasing function of f. The f dependent HPM upset susceptibility model is verified to be reliable and able to quickly estimate the susceptibility level of CMOS inverter considering IC technology, layout parameters, pulse properties, and operating environment simultaneously. Besides, the empirical formula P = A · fαis proposed to provide instant estimation to the HPM upset power threshold. In conclusion, by aid of the analytical model, we acquired the influence of the layout parameter LB on the HPM susceptibility and demonstrated that the CMOS inverter with minor LB is more susceptible to HPM. | YU XinHai CHAI ChangChun LIU Yang YANG YinTang | 2015 | Science China(Information Sciences)2015，58，8： | 4 |
| 18 | Applying various algorithms for species distribution modelling显示文摘Species distribution models have been used extensively in many fields,including climate change biology,landscape ecology and conservation biology.In the past 3 decades,a number of new models have been proposed,yet researchers still find it difficult to select appropriate models for data and objectives.In this review,we aim to provide insight into the prevailing species distribution models for newcomers in the field of modelling.We compared 11 popular models,including regression models(the generalized linear model,the generalized additive model,the multivariate adaptive regression splines model and hierarchical modelling),classification models(mixture discriminant analysis,the generalized boosting model,and classification and regression tree analysis)and complex models(artificial neural network,random forest,genetic algorithm for rule set production and maximum entropy approaches).Our objectives are:(i)to compare the strengths and weaknesses of the models,their characteristics and identify suitable situations for their use(in terms of data type and species-environment relationships)and(ii)to provide guidelines for model application,including 3 steps:model selection,model formulation and parameter estimation. | Xinhai LI Yuan WANG | 2013 | Integrative Zoology2013，8，2： | 3 |
| 19 | RNA-seq analysis of unintended effects in transgenic wheat overexpressing the transcription factor GmDREB1显示文摘The engineering of plants with enhanced tolerance to abiotic stresses typically involves complex multigene networks and may therefore have a greater potential to introduce unintended effects than the genetic modification for simple monogenic traits. For this reason, it is essential to study the unintended effects in transgenic plants engineered for stress tolerance. We selected drought-and salt-tolerant transgenic wheat overexpressing the transcription factor, GmDREB1, to investigate unintended pleiotropic effects using RNA-seq analysis. We compared the transcriptome alteration of transgenic plants with that of wild-type plants subjected to salt stress as a control. We found that GmDREB1 overexpression had a minimal impact on gene expression under normal conditions.GmDREB1 overexpression resulted in transcriptional reprogramming of the salt response,but many of the genes with differential expression are known to mitigate salt stress and contribute incrementally to the enhanced stress tolerance of transgenic wheat. GmDREB1 overexpression did not activate unintended gene networks with respect to gene expression in the roots of transgenic wheat. This work is important for establishing a method of detecting unintended effects of genetic engineering and the safety of such traits with the development of marketable transgenic crops in the near future. | Qiyan Jiang Fengjuan Niu Xianjun Sun Zheng Hu Xinhai Li Youzhi Ma Hui Zhang | 2017 | The Crop Journal2017，5，3： | 3 |
| 20 | A novel threshold-based quadrature signaling scheme for cooperative networks显示文摘Presented is a novel threshold-based quadrature signaling(TQS)scheme for cooperative networks.By assigning different components of the quadrature signaling to different cooperative users and letting each user node decide whether to relay information for the other in the assigned component based on a threshold or not,independently,the TQS scheme effectively integrated the advantages of both quadrature signaling and threshold techniques.The authors investigate the end-to-end bit error rate(BER)performance and obtain its closed-form expression.According to our theoretical analysis,the TQS scheme can enhance the diversity gain of a cooperative system and,meanwhile,reduce the complexity of its implementation to a great extent.In addition,the communication system,using TQS scheme,has the feature of easily switching between cooperative and non-cooperative patterns.Finally,the numerical and simulation results show that the scheme has excellent performance as expected. | SONG XinHai GE JianHua SHI JingJing LI Jing | 2014 | Science China(Information Sciences)2014，57，6： | 3 |
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