|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Dual-specificity histone demethylase KIAA1718 (KDM7A) regulates neural differentiation through FGF4显示文摘histone H3 离氨酸的 Dimethylations 9 和离氨酸 27 是与抄写压抑联系的重要 epigenetic 标记。这里,我们作为为这二个镇压标记特定的新奇 histone demethylase 识别了 KIAA1718 (KDM7A ) 。用老鼠胚胎的干细胞,我们证明那 KIAA1718 表情在神经区别的早阶段增加了。基因击倒堵住的神经区别和效果被野类型的人的基因,并且不由催化地不活跃的异种救。另外, KIAA1718 的 overexpression 加速了神经区别。我们提供 KDM7A 的支持 neural 区别效果通过 FGF4 的直接 transcriptional 激活被调停的证据,一个信号分子在神经区别含有。因此,我们的学习识别了通过 FGF4 调整神经区别的双特性的 histone demethylase。 | Chengyang Huang Yang Xiang Yanru Wang Xia Li Longyong Xu Ziqi Zhu Ting Zhang Qingqing Zhu Kejing Zhang Naihe Jing Charlie Degui Chen | 2010 | Cell Research2010，20，2： | 15 |
| 2 | Genetic characterization and linkage disequilibrium mapping of resistance to gray leaf spot in maize(Zea mays L.)显示文摘Gray leaf spot(GLS),caused by Cercospora zeae-maydis,is an important foliar disease of maize(Zea mays L.)worldwide,resistance to which is controlled by multiple quantitative trait loci(QTL).To gain insights into the genetic architecture underlying the resistance to this disease,an association mapping population consisting of 161 inbred lines was evaluated for resistance to GLS in a plant pathology nursery at Shenyang in 2010 and 2011.Subsequently,a genome-wide association study,using 41,101 single-nucleotide polymorphisms(SNPs),identified 51 SNPs significantly(P<0.001)associated with GLS resistance,which could be converted into 31 QTL.In addition,three candidate genes related to plant defense were identified,including nucleotidebinding-site/leucine-rich repeat,receptor-like kinase genes similar to those involved in basal defense.Two genic SNPs,PZE-103142893 and PZE-109119001,associated with GLS resistance in chromosome bins 3.07 and 9.07,can be used for marker-assisted selection(MAS)of GLS resistance.These results provide an important resource for developing molecular markers closely linked with the target trait,enhancing breeding efficiency. | Liyu Shi Xiangling Lv Jianfeng Weng Hanyong Zhu Changlin Liu Zhuanfang Hao Yu Zhou Degui Zhang Mingshun Li Xiaoke Ci Xinhai Li Shihuang Zhang | 2014 | The Crop Journal2014，2，Z1： | 9 |
| 3 | A sequence of 28S rRNA-derived small RNAs is enriched in mature sperm and various somatic tissues and possibly associates with inflammation显示文摘 | Chu, Chen Yu, Lu Wu, Bin Ma, Li Gou, Lan-Tao He, Miao Guo, Yunli Li, Zhi-Tong Gao, Wei Shi, Huijuan Liu, Mo-Fang Wang, Hongyan Chen, Charlie Degui Drevet, Joel R. Zhou, Yuchuan Zhang, Yonglian | 2017 | Journal of Molecular Cell Biology2017，9，3： | 6 |
| 4 | The histone H3 lysine-27 demethylase Jmjd3 plays a critical role in specific regulation of Th17 cell differentiation显示文摘Interleukin(IL)17-producing T helper(Th17)cells play critical roles in the clearance of extracellular bacteria and fungi as well as the pathogenesis of various autoimmune diseases,such as multiple sclerosis,psoriasis,and ulcerative colitis.Although a global transcriptional regulatory network of Th17 cell differentiation has been mapped recently,the participation of epigenetic modifications in the differentiation process has yet to be elucidated.We demonstrated here that histone H3 lysine-27(H3K27)demethylation,predominantly mediated by the H3K27 demethylase Jmjd3,crucially regulated Th17 cell differentiation.Activation of naı¨ve CD41 T cells immediately induced high expression of Jmjd3.Genetic depletion of Jmjd3 in CD41 T cells specifically impaired Th17 cell differentiation both in vitro and in vivo.Ectopic expression of Jmjd3 largely rescued the impaired differentiation of Th17 cells in vitro in Jmjd3-deficientCD41 T cells.Importantly,Jmjd3-deficient mice were resistant to the induction of experimental autoimmune encephalomyelitis(EAE).Furthermore,inhibition of the H3K27 demethylase activity with the specific inhibitor GSK-J4 dramatically suppressed Th17 cell differentiation in vitro.At the molecular level,Jmjd3 directly bound to and reduced the level of H3K27 trimethylation(me3)at the genomic sites ofRorc,which encodes the masterTh17 transcription factorRorgt,and Th17 cytokine genes such as Il17,Il17f,and Il22.Therefore,our studies established acritical role of Jmjd3-mediatedH3K27demethylation inTh17 cell differentiation andsuggest that Jmjd3 can be a novel therapeutic target for suppressing autoimmune responses. | Zhi Liu Wei Cao Longxia Xu Xi Chen Yu Zhan Qian Yang Sanhong Liu Pengfei Chen Yuhang Jiang Xiaohua Sun Yu Tao Yiming Hu Cuifeng Li Qi Wang Ying Wang Charlie Degui Chen Yufang Shi Xiaoren Zhang | 2015 | Journal of Molecular Cell Biology2015，7，6： | 6 |
| 5 | The Main Content,Technical Support and Enforcement Strategy of Digital Agriculture显示文摘This paper deals with the content,purpose and significance of digital agriculture,and gives a brief introduction to digital earth,digital China and digital agriculture abroad.The main content of digital agriculture includes the construction of database,Metadata standard,monitoring system,forecasting and decision_making system,information_releasing system.The main technical supports of digital agriculture are 3S technology,Metadata management technology,fast_handling technology of remote sensing,the technology of computer wide_band network and virtual reality,intelligent agriculture machinery and field real_time information collecting technology.The advantages of implementing digital agriculture in China and the problems worthy of attention are pointed out as well. | Liang Yong Lu Xiushan Zhang Degui Liang Fu | 2002 | Geo-Spatial Information Science2002，5，1： | 6 |
| 6 | Identification of Functional Genetic Variations Underlying Drought Tolerance in Maize Using SNP Markers显示文摘Single nucleotide polymorphism (SNP) is a common form of genetic variation and popularly exists in maize genome. An Illumina GoldenGate assay with 1 536 SNP markers was used to genotype maize inbred lines and identified the functional genetic variations underlying drought tolerance by association analysis. Across 80 lines, 1 006 polymorphic SNPs (65.5% of the total) in the assay with good call quality were used to estimate the pattern of genetic diversity, population structure, and familial relatedness. The analysis showed the best number of fixed subgroups was six, which was consistent with their original sources and results using only simple sequence repeat markers. Pairwise linkage disequilibrium (LD) and association mapping with phenotypic traits investigated under water-stressed and well-watered regimes showed rapid LD decline within 100-500 kb along the physical distance of each chromosome, and that 29 SNPs were associated with at least two phenotypic traits in one or more environments, which were related to drought-tolerant or drought-responsive genes. These drought-tolerant SNPs could be converted into functional markers and then used for maize improvement by marker-assisted selection. | Zhuanfang Hao Xinhai Li Chuanxiao Xie Jianfeng Weng Mingshun Li Degui Zhang Xiaoling Liang Lingling Liu Sisi Liu Shihuang Zhang | 2011 | Journal of Integrative Plant Biology2011，53，8： | 5 |
| 7 | Prevalence and phylogenetic characterization of canine coronavirus from diseased pet dogs in Beijing,China显示文摘Dear Editor,Canine coronavirus including canine coronavirus(CCoV)and canine respiratory coronavirus(CRCoV)have been recognized as pathogenic viruses to dogs worldwide,causing enteric and respiratory issues(Buonavoglia et al.,2006).Zoonotic transfer of the viruses from the animal kingdom to humans has been repeatedly observed over the past decade, | Shuai Lu Di Zhang Jianfang Zhou Zhaofei Xia Degui Lin Yongliang Lou Wenjie Tan Kun Qin | 2016 | Science China(Life Sciences)2016，59，8： | 5 |
| 8 | PhieCBEs:Plant High-Efficiency Cytidine Base Editors with Expanded Target Range显示文摘Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)-mediated genome editing can efficiently produce gene-knockout mutants.On the other hand,CRISPR/Cas-derived base editors offer the ability to induce precise nucleotide substitutions(Komor et al.,2016).Cytidine base editors(CBEs)consist of a cytidine deaminase fused with a Cas9-nickase variant(Cas9n,with a D10A substitu-tion)and can achieve site-specific C-to-T substitution.Similarly,adenine base editors use an adenine deaminase forA-to-G substi-tution.These systems have been used in various organisms(Mishra et al.,2019).However,the Cas9 complex requires target sites containing NGG protospacer adjacent motifs(PAMs),thus restricting selection of potential targets.A number of CBEs have been developed using Cas9 variants(mostly Cas9n),cytidine deaminases(such as rAPOBEC1 and PmCDA1),and uracil glycosylase inhibitor(UGI)domains.These CBEs of the first generation(BE1,rAPOBEC1-dCas9),second generation(BE2,rAPOBEC1-dCas9-UGI),and third generation(BE3,rAPOBEC1-Cas9n-UGI)have moderate editing efficiencies in mammalians(Komor etal.,2016). | Dongchang Zeng Taoli Liu Jiantao Tan Yaling Zhang Zhiye Zheng Bin Wang Degui Zhou Xianrong Xie Menghui Guo Yao-Guang Liu Qinlong Zhu | 2020 | Molecular Plant2020，13，12： | 5 |
| 9 | Structural insights into a dual-specificity histone demethylase ceKDM7A from Caenorhabditis elegans显示文摘Histone 离氨酸 methylation 能被 JmjC 包含域的蛋白质在 sequence-state-specific 和 methylation-state-specific 举止移开。然而,底层特性怎么样,决定,酶怎么被调整,大部分是未知的。我们最近发现了那 ceKDM7A,一个哲学博士 -- 并且 JmjC 包含域的蛋白质,是为 H3K9me2 特定的 histone demethylase, H3K27me2,和 PHD 摸绑定到 H3K4me3 指南在 vivo 的 demethylation 活动。为了为酶的活动和 PHD 的功能提供结构的卓见进分子的机制,摸,我们与包含 H3K4me3, H3K9me2,和 H3K27me2 修正的各种各样的联合的单身者或二肽一起在 apo 形式并且在建筑群解决了酶的六水晶结构。结构显示 H3K9me2 和 H3K27me2 以一种类似的方式与 ceKDM7A 交往,并且肽绑定特性被特定的相互作用的一个网络决定。结构的几何测量也揭示了与 PHD 手指和 H3K9me2 界限联系到 JmjC 领域的那 H3K4me3 从二个分开的分子,建议 trans-histone 肽绑定机制。因此,我们的全身的结构的研究重要地由催化领域而且更多揭示底层识别不仅,为 H3K9me2 和 H3K27me2 的 ceDKM7A 的双特性的分子的机制。 | Ying Yang Lulu Hu Ping Wang Haifeng Hou Yan Lin Yi Liu Ze Li Rui Gong Xiang Feng Lu Zhou Wen Zhang Yuhui Dong Huirong Yang Hanqing Lin Yiqin Wang Charlie Degui Chen Yanhui Xu | 2010 | Cell Research2010，20，8： | 3 |
| 10 | Dataset of human motion status using IR-UWB through-wall radar显示文摘Ultra-wideband(UWB)through-wall radar has a wide range of applications in non-contact human information detection and monitoring.With the integration of machine learning technology,its potential prospects include the physiological monitoring of patients in the hospital environment and the daily monitoring at home.Although many target detection methods of UWB through-wall radar based on machine learning have been proposed,there is a lack of an opensource dataset to evaluate the performance of the algorithm.This published dataset is measured by impulse radio UWB(IR-UWB)through-wall radar system.Three test subjects are measured in different environments and several defined motion status.Using the presented dataset,we propose a human-motion-status recognition method using a convolutional neural network(CNN),and the detailed dataset partition method and the recognition process flow are given.On the well-trained network,the recognition accuracy of testing data for three kinds of motion status is higher than 99.7%.The dataset presented in this paper considers a simple environment.Therefore,we call on all organizations in the UWB radar field to cooperate to build opensource datasets to further promote the development of UWB through-wall radar. | ZHU Zhengliang YANG Degui ZHANG Junchao TONG Feng | 2021 | Journal of Systems Engineering and Electronics2021，32，5： | 2 |
| 11 | Highly sensitive fluorescence detection of chloride ion in aqueous solution with Ag-modified porous g-C_(3)N_(4) nanosheets显示文摘The porous g-C_(3)N_(4)(PCN)nanosheets are successfully synthesized and further modified with nano-sized Ag by a simple wet-chemical process.Intere stingly,the Ag-modified porous g-C_(3)N_(4)(Ag-PCN)nanosheets exhibit competitive fluorescence detection performance of chloride ion(Cl)in aqueous solutio n.Under the optimized conditions,the concentration of Cl could be quantitative analyzed with the Ag-PCN in a wide detection range from 0.5 mmol/L to 0.1 mol/L,with a low detection limitation of 0.06 mmol/L.It is confirmed that the fluorescence of PCN could be effectively decayed by the photoinduced charge transfer via the adsorbed Cl for trapping holes,mainly by means of the time-resolved fluorescence and surface photo voltage spectra.The porous structure and modified Ag promote the adsorption of Cl on resulting Ag-PCN,leading to excellent fluorescence detection for Cl.This work provides a feasible route to develop a fluorescence detection of Cl with g-C_(3)N_(4) nanosheets in environment water. | Zishu Zhang Ying Gao Peng Li Binhong Qu Zhiyuan Mu Yang Liu Yang Qu Degui Kong Qing Chang Liqiang Jing | 2020 | Chinese Chemical Letters2020，31，10： | 2 |
| 12 | Knockout of glutathione peroxidase 5 down-regulates the piRNAs in the caput epididymidis of aged mice显示文摘The mammalian epididymis not only plays a fun dame ntal role in the maturati on of spermatozoa,but also provides protecti on agai nst various stressors.The foremost among these is the threat posed by oxidative stress,which arises from an imbalance in reactive oxygen species and can elicit damage to cellular lipids,proteins,and nucleic acids.In mice,the risk of oxidative damage to spermatozoa is mitigated through the expression and secretion of glutathione peroxidase 5(GPX5)as a major luminal scavenger in the proximal caput epididymidal segment.Accordingly,the loss of GPX5^-/-mediated protection leads to impaired DNA integrity in the spermatozoa of aged Gpx57 mice.To explore the underlying mechanism,we have conducted transcriptomic analysis of caput epididymidal epithelial cells from aged(13 months old)Gpx5^-/-m mice.This analysis revealed the dysregulation of several thousand epididymal mRNA transcripts,in eluding the downregulation of a subgroup of piRNA pathway gen es,in aged Gpx5^-/-mice.In agreeme nt with these fin dings,we also observed the loss of piRNAs,which potentially bind to the P-element-induced wimpy testis(PlWI)-like proteins PIWIL1 and PIWIL2.The absence of these piRNAs was correlated with the elevated mRNA levels of their putative gene targets in the caput epididymidis of Gpx5^-/-mice.Importantly,the oxidative stress response genes tend to have more targeting piRNAs,and many of them were among the top increased genes upon the loss of GPX5^-/-.Taken together,our findings suggest the existence of a previously uncharacterized somatic piRNA pathway in the mammalian epididymis and its possible invoIvement in the aging and oxidative stress-mediated responses. | Chen Chu Lu Yu Joelle Henry-Berger Yan-Fei Ru Ayhan Kocer Alexandre Champroux Zhi-Tong Li Miao He Sheng-Song Xie Wu-Bin Ma Min-Jie Ni Zi-Mei Ni Yun-Li Guo Zhao-Liang Fei Lan-Tao Gou Qiang Liu Samanta Sharma Yu Zhou Mo-Fang Liu Charlie Degui Chen Andrew L Eamens Brett Nixon Yu-Chuan Zhou Joel R Drevet Yong-Lian Zhang | 2020 | Asian Journal of Andrology2020，22，6： | 2 |
| 13 | Kernel lysine content does not increase in some maize opaque2 mutants显示文摘 | Gang Zhao Mingshun Li Degui Zhang Xinhai Li Zikai Wu Xiaoke Ci Chuanxiao Xie Li Bai Zhenyu Lu Liang Chen Zhuanfang Hao Shihuang Zhang | 2012 | Planta2012，，1： | 1 |
| 14 | Genetic and Phenotypic Investigation of a Chinese Pedigree with Lattice Corneal Dystrophy ⅢB Subtype显示文摘Purpose:.To investigate phenotypes and disease-causing mutation in the transforming growth factor b-induced gene(TGFBI) in a Southern Chinese pedigree with lattice corneal dystrophy(LCD) IIIB with complicated cataract.Methods:.A Southern Chinese pedigree with lattice corneal dystrophy IIIB with complicated cataract was recruited. Comprehensive ophthalmic investigations were performed before and after cataract surgery of phacoemulsification and intraocular lens implantation in the proband's both eyes..Peripheral blood was collected from the proband,.and genomic DNA was extracted..All exons of the TGFBI gene were sequenced to screen possible mutations.Results:.A bilateral LCD IIIB subtype was observed in the proband..Optical coherence tomography further revealed superreflective changes in the subepithelial and stroma layers of the cornea,.with reduced central corneal thickness..Notably,bilateral cataract was found in the proband..Direct sequencing detected a recurrent heterozygous missense c.1877A>G mutation in exon 14 of the TGFBI gene,.resulting in substitution of histidine with arginine(p.H626R).Conclusion:.The current study was the first report of the TGFBI p.H626R mutation in Southern Chinese,.suggesting that it could be a mutation hotspot across populations..Moreover,.the mutation was associated with LCD IIIB subtype with complicated cataract,.which had not been reported before,pointing to clinical heterogeneity of the mutation. | Degui Wang Yong Yao Mingzhi Zhang Jianhuan Chen | 2013 | Eye Science2013，28，3： | 1 |
| 15 | Research on simula- tion of the interruption of low-voltage circuit breaker显示文摘 | ZHANG Jin CHEN Degui FU Jun | 1999 | Journal of Xi''an Jiaotong University1999，，1： | 1 |
| 16 | A new panel containing specific spermatogenesis markers to identify spermatogenic cells in nonobstructive azoospermia patients显示文摘Infertility is a severe problem that affects 10%-15% of couples worldwide, among which male infertility is a factor in ~50% of the cases [1]. Azoospermia, which is defined as the absence of sperm in at least two ejaculates, can be divided into two types: obstructive azoospermia (OA) and non-OA (NOA)[1]. OA patients have normal spermatogenesis and mature sperm in testes and can father a biological child through testicular sperm retrieval and intracytoplasmic sperm injection (ICSI)[2]. By contrast, NOA patients usually have various degrees of spermatogenesis impairment and can be faced with complicated assisted reproductive technology (ART) strategies to father a biological child [2]. The precise diagnosis of spermatogenesis in NOA patients is crucial for clinical decision making regarding ART. | Xinxi Zhao Yiqin Wang Meng Ma Wei Zhang Jiqiang Si Wei Wang Zhen Lin Gang Han Charlie Degui Chen Minghan Tong Yanping Kuang Qifeng Lyu | 2019 | Acta Biochimica et Biophysica Sinica2019，51，6： | 1 |
| 17 | Problems and Countermeasures in Wumeng Native Chicken Industry显示文摘Wumeng native chicken( Wumeng silky chicken and Weining chicken) grow mainly in Wumeng mountainous area of Yunnan-Guizhou plateau. Wumeng silky chicken is featured by strong stress resistance,delicious meat,abundant essential restrictive amino acids and trace elements for human body( zinc,selenium,etc.),with high medicinal and health value. Weining chicken is characterized by early sexual maturity,uniform distribution of intramuscular fat,small muscle fiber,tender and delicious meat,resistance to cold and forage,which is an excellent local chicken breed for development of modern,efficient,mountainous ecological animal husbandry in Bijie area. The existing problems in industrialization development and utilization of Wumeng native chicken are discussed,and the corresponding countermeasures are put forward. | Zhang Xiben Li Weibo Ou Maojun Li Degui | 2017 | Animal Husbandry and Feed Science2017，9，2： | 0 |
| 18 | Highly dispersed silver nanoparticles for performance-enhanced lithium oxygen batteries显示文摘Aprotic lithium-oxygen batteries possess ultrahigh energy density but suffer from the sluggish decomposition of discharge product,quick depletion of Li anode and cleavage of electrolyte,in close association with oxygen reduction reaction at the cathode.Herein,highly dispersed silver nanoparticles are used to enhance the lithium-oxygen battery with 1.0 M lithium perchlorate in dimethyl sulfoxide.It is observed that film-like amorphous lithium peroxide is formed through surface pathway instead of bulk crystals,due to the incorporation of silver nanoparticles dispersed in the electrolyte,which subsequently accelerates the decomposition of the discharge product by offering more active sites and improved conductivity.The released silver nanoparticles after battery charging can be re-used in the following cycles.Experiments and theoretical calculation further indicate that the suspended silver nanoparticles can adsorb the soluble oxygen reduction intermediates,which are responsible for the alleviation of oxidative cleavage of electrolyte and corrosion of lithium anode.The lifespan of lithium oxygen batteries is therefore significantly extended from 55 to 390 cycles,and the rate performance and full-discharge capacity are also largely enhanced.The battery failure is attributed to the coalescence and growth of silver nanoparticles in the electrolyte,and further improvement on colloid stability is underway. | Zhihong Luo Fujie Li Chengliang Hu Liankun Yin Degui Li Chenhao Ji Xiangqun Zhuge Kui Zhang Kun Luo | 2021 | Journal of Materials Science & Technology2021，，14： | 0 |
| 19 | JMJD3 promotes chondrocyte proliferation and hypertrophy during endochondral bone formation in mice显示文摘JMJD3(KDM6B)is an H3K27me3 demethylase and counteracts polycomb-mediated transcription repression.However,the function of JMJD3 in vivo is not well understood.Here we show that JMJD3 is highly expressed in cells of the chondrocyte lineage,especially in prehypertrophic and hypertrophic chondrocytes,during endochondral ossification.Homozygous deletion of Jmjd3 results in severely decreased proliferation and delayed hypertrophy of chondrocytes,and thereby marked retardation of endochondral ossification in mice.Genetically,JMJD3 associates with RUNX2 to promote proliferation and hypertrophy of chondrocytes.Biochemically,JMJD3 associates with and enhances RUNX2 activity by derepression of Runx2 and Ihh transcription throughits H3K27me3 demethylase activity.These results demonstrate that JMJD3 is a key epigenetic regulator in the process of cartilage maturation during endochondral bone formation. | Feng Zhang Longyong Xu Longxia Xu Qing Xu Dangsheng Li Yingzi Yang Gerard Karsenty Charlie Degui Chen | 2015 | Journal of Molecular Cell Biology2015，7，1： | 0 |
| 20 | Exponentially reduced carrier mobility of natural ester via blocking effect of 2D hexagonal boron nitride nanosheets显示文摘In this work,the boron nitride(BN)nanosheets were dispersed in natural esters to fabricate the dielectric nanofluid.Microstructures and chemical compositions of the nanosized BN are determined.The stability,viscosity,and thermal conductivity of the BN nanofluid,were obtained.And the dissipation factor,electrical conductivity,and relative permittivity of the BN nanofluid,were measured.The ion mobilities and migrating times of the nanofluid were measured under different volumetric fractions of BN nanosheets and temperatures.Results show that the electrical conductivity and dissipation factor of the nanofluid decreased by 54%and 48%with the addition of only 0.1%of BN nano-sheets under 110°C.Both the half-reduced carrier mobility of the nanofluid and the declined ion concentration by blocking of BN nanosheet contributed to the exponentially reduced electrical conductivity and enhanced dielectric performances of the BN nano-fluid.The suppression coefficient k is proposed to quantitatively describe the hinder effect of ion migration in nanofluids by 2D BN nanosheet.Results provide a strategy to design and develop advanced nanofluids with low-dielectric loss by aids of surface adsorption of ion and steric hindrance of 2D nanosheets. | Zhengyong Huang Gang He Jian Li Feipeng Wang Ruiqi Zhang Degui Yao | 2021 | High Voltage2021，6，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员