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| 1 | Identification of an epitope of SARS-coronavirus nucleocapsid protein显示文摘The nucleocapsid (N) protein of severe acute respiratory syndrome-coronavirus (SARS-CoV) is a majorvirion structural protein. In this study, two epitopes (N1 and N2) of the N protein of SARS-CoV werepredicted by bioinformatics analysis. After immunization with two peptides, the peptides-specific antibodieswere isolated from the immunized rabbits. The further experiments demonstrated that N1 peptide-inducedpolyclonal antibodies had a high affinity to bind to E. coli expressed N protein of SARS-CoV. Furthermore, itwas confirmed that N1 peptide-specific IgG antibodies were detectable in the sera of severe acute respiratorysyndrome (SARS) patients. The results indicated that an epitope of the N protein has been identified andN protein specific Abs were produced by peptide immunization, which will be useful for the study of SARS-CoV. | YINGLIN XuSHEN RUIFuYANG YIXUELI YONGYONGJI YouYuHE MuDESHI WEILU TIELIUSHI BINGSUN JINWANG HONGXIAWANG HUALIANGJIANG JIANHUASHEN YOUHUAXIE YUANWANG GANGPEI BBIFENSHEN JIARUIWU | 2003 | Cell Research2003，13，3： | 22 |
| 2 | Activity criterion of pre-existing fabrics in non-homogeneous deformation domain显示文摘The Anderson's model can be applied only to elastic homogeneous deformation and cannot explain complicated phenomena of natural faults, which to a large degree limits the model to practical application. By combing the Coulomb-Mohr Criterion with the sandbox modeling and considering non-homogeneous deformation, mechanisms of how basement pre-existing fabrics control fault formation and evolution are analyzed and a mechanical factor, activation-coefficient (faS) of pre-existing fabrics, is proposed. It is determined by the attitude and mechanical properties of pre-existing fabric, and the stress state (the magnitudes and directions of the three principal stresses). The coefficient has taken the heterogeneity of rocks into account and may serve as a criterion for evaluating the activity of a pre-existing fabric. The Mohr-Coulomb Criterion is expanded to non-homogeneous deformation domain in terms of activation-coefficient (faS) of pre-existing fabrics, the general law of the activity of a pre-existing fabric is predicted, the fault complexity real of rift basin is revealed in theory, and the controlling law of basement pre-existing faults to fault formation and evolution is determined, and checked with sandbox modeling. A new way is provided for in-depth study of faulting. | TONG HengMao CAI DongSheng WU YongPing LI XiaoGuang LI XuShen MENG LingJian | 2010 | Science China Earth Sciences2010，53，8： | 17 |
| 3 | Geology and hydrocarbon accumulations in the deepwater of the northwestern South China Sea——with focus on natural gas显示文摘The deepwater of the northwestern South China Sea is located in the central to southern parts of the Qiongdongnan Basin(QDN Basin),which is a key site for hydrocarbon exploration in recent years.In this study,the authors did a comprehensive analysis of gravity-magnetic data,extensive 3D seismic survey,cores and cuttings,paleontology and geochemical indexes,proposed the mechanism of natural gas origin,identified different oil and gas systems,and established the model of hydrocarbon accumulations in the deep-water region.Our basin tectonic simulation indicates that the evolution of QDN Basin was controlled by multiple-phased tectonic movements,such as Indochina-Eurasian Plate collision,Tibetan Uplift,Red River faulting and the expansion of the South China Sea which is characterized by Paleogene rifting,Neogene depression,and Eocene intensive faulting and lacustrine deposits.The drilling results show that this region is dominated by marineterrestrial transitional and neritic-bathyal facies from the early Oligocene.The Yacheng Formation of the early Oligocene is rich in organic matter and a main gas-source rock.According to the geological-geochemical data from the latest drilling wells,Lingshui,Baodao,Changchang Sags have good hydrocarbon-generating potentials,where two plays from the Paleogene and Neogene reservoirs were developed.Those reservoirs occur in central canyon structural-lithologic trap zone,Changchang marginal trap zone and southern fault terrace of Baodao Sag.Among them,the central canyon trap zone has a great potential for exploration because the various reservoirforming elements are well developed,i.e.,good coal-measure source rocks,sufficient reservoirs from the Neogene turbidity sandstone and submarine fan,faults connecting source rock and reservoirs,effective vertical migration,late stage aggregation and favorable structural–lithological composite trapping.These study results provide an important scientific basis for hydrocarbon exploration in this region,evidenced by the recent discovery of the significant commercial LS-A gas field in the central canyon of the Lingshui Sag. | WANG Zhenfeng SUN Zhipeng ZHANG Daojun ZHU Jitian LI Xushen HUANG Baojia GUO Minggang JIANG Rufeng | 2015 | Acta Oceanologica Sinica2015，34，10： | 9 |
| 4 | Geochemistry of Pore Water and Associated Diagenetic Reactions in the Diapiric Area of Yinggehai Basin, Northwestern South China Sea显示文摘这研究在 Yinggehai 盆的 diapiric 区域检验了毛孔水的 geochemical 特征,西北的华南海,并且照亮了盆液体的起源和进化。甚至没有大气的水渗入,有低咸度的毛孔水在 diapiric 区域发生在海洋的沉积。在内的低咸度的水的存在深, overpressured 分隔空间被假定由于 smectite-illite 转变。Howerver 在有正常压力的 diapiric 区域的浅部分(不到 2 000 m ) ,毛孔水在 overpressured 间隔比那有一个宽得多的变化和低得多的咸度。它的全部的溶解固体(TDS ) 内容是 5 336 ~ 35 939 mg/L。而且,当 kaolinite 和 illite 在更浅的间隔满足增加,绿土和绿泥石内容严厉地减少。在 diapiric 结构的毛孔水的 geochemical 变化显示逐出低咸度,沿着垂直差错的 overpressured 液体。从深 overpressured 沉积的热液体的强壮的注射在浅水库导致快速的泥土矿物质转变。因而,从更深的 overpressured 沉积物由于液体逐出混合的液体象一些 diagenetic 反应一样在咸度和离子的作文导致变化。这包括更深热的液体的更高的 temperatur 引起的泥土矿物质的转变,例如,到 illite 的绿土和到 kaolinite 的绿泥石的 transfromation。因此,在咸度的变化和在各种各样的迫使的系统的离子的作文提供线索给流动小径和联系 diagenetic 反应。 | Tao Jiang Xinong Xie Hui Chen Zhenfeng Wang Xushen Li | 2015 | Journal of Earth Science2015，26，3： | 6 |
| 5 | Difference in full-filled time and its controlling factors in the Central Canyon of the Qiongdongnan Basin显示文摘Based on the interpretation of high resolution 2D/3D seismic data,sedimentary filling characteristics and fullfilled time of the Central Canyon in different segments in the Qiongdongnan Basin of northwestern South China Sea have been studied.The research results indicate that the initial formation age of the Central Canyon is traced back to 11.6 Ma(T40),at which the canyon began to develop due to the scouring of turbidity currents from west to east.During the period of 11.6–8.2 Ma(T40–T31),strong downcutting by gravity flow occurred,which led to the formation of the canyon.The canyon fillings began to form since 8.2 Ma(T31) and were dominated by turbidite deposits,which constituted of lateral migration and vertical superposition of turbidity channels during the time of8.2–5.5 Ma.The interbeds of turbidity currents deposits and mass transport deposits(MTDs) were developed in the period of 5.5–3.8 Ma(T30–T28).After then,the canyon fillings were primarily made up of large scale MTDs,interrupted by small scale turbidity channels and thin pelagic mudstones.The Central Canyon can be divided into three types according to the main controlling factors,geomorphology-controlled,fault-controlled and intrusionmodified canyons.Among them,the geomorphology-controlled canyon is developed at the Ledong,Lingshui,Songnan and western Baodao Depressions,situated in a confined basin center between the northern slope and the South Uplift Belt along the Central Depression Belt.The fault-controlled canyon is developed mainly along the deep-seated faults in the Changchang Depression and eastern Baodao Depression.Intrusion-modified canyon is only occurred in the Songnan Low Uplift,which is still mainly controlled by geomorphology,the intrusion just modified seabed morphology.The full-filled time of the Central Canyon differs from west to east,displaying a tendency of being successively late eastward.The geomorphology-controlled canyon was completely filled before3.8 Ma(T28),but that in intrusion-modified canyon was delayed to 2.4 Ma(T27) because of the uplifted southern canyon wall.To the Changchang Depression,the complete filling time was successively late eastward,and the canyon in eastern Changchang Depression is still not fully filled up to today.Difference in full-filled time in the Central Canyon is mainly governed by multiple sediment supplies and regional tectonic activities.Due to sufficient supply of turbidity currents and MTDs from west and north respectively,western segment of the Central Canyon is entirely filled up earlier.Owing to slower sediment supply rate,together with differential subsidence by deep-seated faults,the full-filled time of the canyon is put off eastwards gradually. | SHANG Zhilei XIE Xinong LI Xushen ZHANG Daojun HE Yunlong YANG Xing CUI Mingzhe | 2015 | Acta Oceanologica Sinica2015，34，10： | 5 |
| 6 | Enzymatic activity characterization of SARS coronavirus 3C-like protease by fluorescence resonance energy transfer technique显示文摘Aim: To characterize enzymatic activity of severe acute respiratory syndrome (SARS) coronavirus (CoV) 3C-like protease (3CL^pro) and its four site-directedmutants. Methods: Based on the fluorescence resonance energy transfer (FRET)principle using 5-[(2'-aminoethyl)-amino] naphthelenesulfonic acid (EDANS) and4-[[4-(dimethylamino) phenyl] azo] benzoic acid (Dabcyl) as the energy transferpair, one fluorogenic substrate was designed for the evaluation of SARS-CoV3CL^pro proteolytic activity. Results: The kinetic parameters of the fluorogenicsubstrate have been determined as Kin=404μmol·L^-1, kcat=1.08min^-1, and kcat/Km=2.7mmol^-1·L·min^-1. SARS-CoV 3CL^pro showed substantial pH and temperature-triggered activity switches, and site-directed mutagenesis analysis of SARS-CoV3CL^pro revealed that substitutions of His^41, Cys^145, and His^163 resulted in complete loss of enzymatic activity, while replacement of Met^162 with Ala caused strongly increased activity. Conclusion: This present work has provided valuable information for understanding the catalytic mechanism of SARS-CoV 3CL^pro. This FRET-based assay might supply an ideal approach for the exploration SARS-CoV 3CL^pro putative inhibitors. | ShuaiCHEN Li-liCHEN Hai-binLUO TaoSUN,JingCHEN JingCHEN FeiYIE Jian-huaCAI Jing-kangSHEN XuSHEN Hua-liangJIANG | 2005 | Acta Pharmacologica Sinica2005，26，1： | 3 |
| 7 | Characteristics and distribution patterns of reef complexes on the carbonate platform margin in deep water areas:the western South China Sea显示文摘As a potential oil and gas reservoir,reef complexes have been a research focus from petroleum geologists for a long time.There are favorable conditions for the development of reef complexes in the South China Sea; however,their internal structures,evolution and distribution are still poorly understood.Based on 2D and 3D seismic data,the internal structures and evolution patterns of the reef complexes on the carbonate platform margin in the deep water areas over the western South China Sea were studied in detail.The result shows that two types of reef complexes,i.e.,fault controlling platform margin reef complexes and ramp reef complexes have been developed in the study area.The reef complexes have independent or continuous mound or lenticular seismic reflections,with three internal structures(i.e.,aggrading,prograding and retrograding structures).There are different growth rates during the evolution of the reef complexes,resulting in the formation of catch-up reefs,keep-up reefs and quick step reefs.The study also reveals that different platform margin reef complexes have different internal structures and distributions,because of the different platform types.These results may be applied to the exploration and prediction of carbonate platform margin reef complexes in other areas that are similar to the study area. | CHEN Ping LI Xushen WANG Yahui LU Yongchao ZHONG Zehong CHEN Lei ZUO Qianmei MA Yiquan WANG Chao DU Xuebin | 2015 | Acta Oceanologica Sinica2015，34，10： | 3 |
| 8 | Epitranscriptomics:Toward A Better Understanding of RNA Modifications显示文摘Ever since the first RNA nucleoside modification was characterized in 1957~[1],over 100 distinct chemical modifications have been identified in RNA to date~[2].Most of these modifications were characterized in non-coding RNAs(nc RNAs),including t RNA,r RNA,and small nuclear RNA(sn RNA)~[3].Studies in the past few decades have located various modifications in these nc RNAs and revealed their functional roles~[3].For instance,N1-methyladenosine(m^1A),which is | Xushen Xiong Chengqi Yi Jinying Peng | 2017 | Genomics, Proteomics & Bioinformatics2017，15，3： | 3 |
| 9 | Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori显示文摘 | CongHan LiruiWang KunqianYu LiliChen LihongHu KaixianChen HualiangJiang XuShen | 2006 | FEBS Journal2006，，： | 1 |
| 10 | Conformational flexibility of β-secretase: molecular dynamics simulation and essential dynamics analysis显示文摘AIM: Based on the structural analysis to reveal the mechanism of ligand binding to b-secretase and the specificityof each binding sub-site. METHODS: Molecular dynamics was used to simulate on the ligand free b-secretase andligand bound b-secretase. The trajectories were analyzed using the essential dynamics, and the significant confor-mational change was illustrated employing the DynDom program. RESULTS: The essential dynamics and DynDomanalyses clearly showed that the b-secretase experienced a large conformational change upon the substrate orinhibitor binding. The flap structure adopted a swing motion, gradually covering the active site to facilitate theligand binding process. Residues Ser86 and Ile87 served as the hinge point. Inhibitor-enzyme interaction analysisrevealed that residues at P2, P1, and P1 positions of the inhibitor were very important for the binding, and residuesat P2 and P3 positions may be modified to improve the binding specificity. S3 subsite of the enzyme still hadspace to modify the inhibitors in increasing the binding affinity. CONCLUSION: The information presented hereis valuable and could be used to identify small molecular inhibitors of b-secretase. | BingXIONG Xiao-qinHUANG Ling-lingSHEN Jian-huaSHEN Xiao-minLUO XuSHEN Hua-liangJIANG Kai-xianCHEN | 2004 | Acta Pharmacologica Sinica2004，25，6： | 1 |
| 11 | 显示文摘 | GuoHongfei(郭洪飞) AnMaozhong(安茂忠) xuShen(徐莘)eta1 | 2005 | 稀有金属材料与工程2005，34，10： | 1 |
| 12 | Swietenia mahagony extract shows agonistic activity to PPAR7 and gives ameliorative effects on diabetic db/db mice显示文摘Aim: To search the peroxisome proliferator-activated receptor T (PPARγ) agonists from Swietenia mahagony extract (SINE) and observe the possible ameliorative effects of SinE on diabetic db/db mice. Methods: The PPARγ agonistic activity of SmE was screened by yeast-two hybrid system. The blood glucose levels of diabetic db/db mice were measured using a blood glucose level monitor and the data were statistically analyzed by NDSTS.SW software. Results: By using the clinical drug rosiglitazone as a positive control, it was found that the PPARγ agonistic activity of SInE at a concentration of 50 ug/L was approximately half that of 35.7 ug/L (0.1 umol/L) of rosiglitazone. At the dose of 1000 mg/kg, SmE remarkably decreased the blood glucose concentration of db/db mice from (15.26±2.98)to (7.58±2.20) mmol/L, and reduced the blood glucose levels by 55.49% compared with the control group (P<0.01). Conclusion: SInE shows agonistic activity to PPART and can ameliorate the blood glucose levels of diabetic db/db mice. SmE may be thus used as a potential agent for diabetes therapy. | Dan-DanLI Jun-huaCHEN QingCHEN Guo-weiLI JingCHEN Jian-minYUE Min-liCHEN Xiao-pingWANG Jian-huaSHEN XuSHEN Hua-liangJIANG | 2005 | Acta Pharmacologica Sinica2005，26，2： | 0 |
| 13 | Isolations and Identifications of Two Psychrotrophs and Preliminary Research on Their Applications显示文摘[Objective]The research aimed to initially study degradation effect of the COD Cr in sewage by two psychrotrophs. [Method] Two psychrotrophs were isolated from the activated sludge of wastewater treatment plant in Tianjin Konggang Economic Area. COD Cr degradation ability of the screened psychrotroph was analyzed in simulated domestic wastewater at 6 ℃. [Result]K36 was identified as Comamonas testosterone,and K38 was identified as Serratia fonticola. COD Cr degradation abilities of the two strains were different in test. COD Cr removal rates of the K36 and K38 respectively reached up to 23% and 53%. The measured result of growth rate suggested that two psychrotrophs both had high activities at low temperature. [Conclusion]K36 and K38 had potentials in wastewater treatment application. | Huifeng Li Jinhui Huang Xushen Zhou Chao Lin Yang Luo | 2013 | Meteorological and Environmental Research2013，4，2： | 0 |
| 14 | 31nstitute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Taiyuan Road,Shanghai 200031, China.显示文摘 Spike protein is one of the major structural proteins of severe acute respiratory syndrome-coronavirus. It is essential for the interaction of the virons with host cell receptors and subsequent fusion of the viral envelop with host cell membrane to allow infection. Some spike proteins of coronavirus, such as MHV, HCoV-OC43, AIBV and BcoV, are proteolytically cleaved into two subunits, S 1 and S2. In contrast, TGV, FIPV and HCoV-229E are not. Many studies have shown that the cleavage of spike protein seriously affects its function. In order to investigate the maturation and proteolytic processing of the S protein of SARS CoV, we generated S 1 and S2 subunit specific antibodies (Abs) as well as N, E and 3CL protein-specific Abs. Our results showed that the antibodies could efficiently and specifically bind to their corresponding proteins from E.coli expressed or lysate of SARS-CoV infected Vero-E6 cells by Western blot analysis. Furthermore, the anti-S 1 and S2 Abs were proved to be capable of binding to SARS CoV under electron microscope observation. When S2 Ab was used to perform immune precipitation with lysate of SARS-CoV infected cells, a cleaved S2 fragment was detected with S2-specific mAb by Western blot analysis. The data demonstrated that the cleavage of S protein was observed in the lysate, indicating that proteolytic processing of S protein is present in host cells. | XiaoDongWU BoSHANG RuiFuYANG HaoYU ZhiHalMA XuSHEN YongYongJI YingLIN YaDiWU GuoMeiLIN LinTIAN XiaoQingGAN ShengYANG WeiHongJIANG ErHeiDAI XiaoYiWANG HuaLiangJIANG YouHuaXIE XueLiangZHU GangPEI LinLI JiaRuiWU BingSUN | 2004 | Cell Research2004，14，5： | 0 |
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