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| 1 | Arabidopsis F-box gene FOA1 involved in ABA signaling显示文摘The expression of FOA1(F-box overexpressed/oppressed ABA signaling) in different organs of Arabidopsis,and in response to ABA and NaCl,was analyzed.The expression level of FOA1 is higher in the root and is lower in the stem,and is induced rapidly by ABA and NaCl.The phenotypes of T-DNA insertion mutant foa1 and FOA1 overexpression lines FOA1ox1 and FOA1ox2 were analyzed.The foa1 mutant exhibited a lower germination rate,shorter root length,more stomatal opening,increased proline accumulation and hypersensitivity to ABA compared with the wild type.In contrast,the overexpression lines showed lower sensitivity to ABA than the wild type.The expression levels of several ABA and stress-responsive transcription factors and genes were altered in the foa1 mutant in response to ABA.Compared with the wild type,the expression levels of ABA-responsive transcription factors were higher,but ABA and stress-responsive genes were lower in foa1 mutant.This study demonstrates that FOA1 is an ABA signaling-related gene,and may play a negative role in ABA signaling. | PENG Juan YU DaShi WANG LiQun XIE MinMin YUAN CongYing WANG Yu TANG DongYing ZHAO XiaoYing LIU XuanMing | 2012 | Science China(Life Sciences)2012，55，6： | 12 |
| 2 | Preparation of folate-conjugated starch nanoparticles and its application to tumor-targeted drug delivery vector显示文摘Anion starch nanoparticles (StNP) were prepared in water-in-oil microemulsion. Folate modi-fied with PEG was conjugated to the surface of StNP to obtain the folate-conjugated starch nanoparticles (FA-PEG/StNP). The average diameter of FA-PEG/ StNP determined by AFM and Zeta-Sizer apparatus was about 130 nm. Doxorubicin (DOX)-loaded FA- PEG/StNP was obtained via infiltrating combination. The result of UV spectrophotometer showed that the saturation concentration of DOX-loaded FA-PEG/ StNP was 28 μg/mg, which was effective for the con-trolled release of anticancer drug DOX. The cell ex-periments showed that the Lc50 of DOX-loaded FA-PEG/StNP and DOX-loaded StNP was higher than that of DOX, which indicates that FA-PEG/StNP and StNP can decrease the toxicity of DOX; while the lethal rate of DOX-loaded FA-PEG/StNP was 3 times that of DOX-loaded StNP with the same quantity of DOX, which indicates that FA in FA-PEG/StNP is ef-fective for improving the targeting function of nanoparticles, thus enhancing the inhibition effect of anticancer drug to cancer cell. This work demon-strates that the FA-PEG/StNP system is a potentially useful system for the targeted delivery of anticancer drug DOX. | XlAO Suyao TONG Chunyi LIU Xuanming YU Danmi LIU Qiaoling XUE Changgang TANG Dongyin ZHAO Lijian | 2006 | Chinese Science Bulletin2006，51，14： | 12 |
| 3 | Coronavirus MHV-A59 infects the lung and causes severe pneumonia in C57BL/6 mice显示文摘It remains challenging to develop animal models of lung infection and severe pneumonia by severe acute respiratory syndrome coronavirus(SARS-CoV) and Middle East respiratory syndrome cornavirus(MERS-Co V) without high level of containment. This inevitably hinders understanding of virushost interaction and development of appropriate countermeasures. Here we report that intranasal inoculation of sublethal doses of murine coronavirus mouse hepatitis virus A-59(MHV-A59), a hepatic and neuronal tropic coronavirus, can induce acute pneumonia and severe lung injuries in C57BL/6 mice. Inflammatory leukocyte infiltrations, hemorrhages and fibrosis of alveolar walls can be observed 2-11 days after MHV-A59 infection. This pathological manifestation is associated with dramatical elevation of tissue IP-10 and IFN-γ and moderate increase of TNF-α and IL-1β, but inability of anti-viral type I interferon response. These results suggest that intranasal infection of MHV-A59 would serve as a surrogate mouse model of acute respiratory distress syndrome by SARS-CoV and MERS-CoV infections. | Zhangsheng Yang Jun Du Gang Chen Jie Zhao Xuanming Yang Lishan Su Genhong Cheng Hong Tang | 2014 | Virologica Sinica2014，29，6： | 9 |
| 4 | Studies of poly-L-lysine-starch nanoparticle preparation and its application as gene carrier显示文摘Anion starch nanoparticle (StNP) with a diameter of 50 nm was prepared in wa- ter-in-oil microemulsion, with soluble starch as raw materials and POCl3 as crosslinking agent. PLL-StNP was prepared by linking poly-L-lysine (PLL) on the surface of StNP. At the same time, the size of PLL-StNP and its stability in aqueous solution were checked by AFM. The analysis of plasmid DNA binding, DNase I enzymatic degradation, toxicity and transfection were done. We discovered that PLL-StNP may be used as non-virus nanoparticle gene carrier. And we devel- oped the method of preparing PLL-StNP gene carrier and used it in cell transfection. As non-virus gene carrier, PLL-StNP has some advantages, such as large load of DNA, high transfection effi- ciency, low cell toxicity and biodegradability. | XIAO Suyao LIU Xuanming TONG Chunyi LIU Jun TANG Dongying ZHAO Lijian | 2005 | Science China Chemistry2005，48，2： | 7 |
| 5 | Ectopic expression of fungal EcGDH improves nitrogen assimilation and grain yield in rice显示文摘NADP(H)-dependent glutamate dehydrogenases(GDH) in lower organisms have stronger ammonium affinity than those in higher plants. Here we report that transgenic rice overexpressing the EcGDH from Eurotium cheralieri exhibited significantly enhanced aminating activities. Hydroponic and field tests showed that nitrogen assimilation efficiency and grain yields were markedly increased in these transgenic plants, especially at the low nitrogen conditions.These results suggest that EcGDH may have potential to be used to improve nitrogen assimilation and grain yield in rice. | Dongying Tang Yuchong Peng Jianzhong Lin Changqing Du Yuanzhu Yang Dan Wang Cong Liu Lu Yan Xiaoying Zhao Xia Li Liangbi Chen Xuanming Liu | 2018 | Journal of Integrative Plant Biology2018，60，2： | 4 |
| 6 | F-box gene FOA2 regulates GA-and ABA-mediated seed germination in Arabidopsis显示文摘Dear Editor,GA and ABA antagonize each other in controlling seed germination,but the molecular mechanism is not fully understood.The F-box proteins act as the most important SCF(SKP1,cullin/CDC53,F-box protein)complex subunit | Reqing Hei Dashi Yu Xinmei Li Guifang Duan Yunxuan Zhang Dongying Tang Xiaoying Zhao Xuanming Liu | 2016 | Science China(Life Sciences)2016，59，11： | 3 |
| 7 | The blue light receptor CRY1 interacts with GID1 and DELLA proteins to repress GA signaling during photomorphogenesis in Arabidopsis显示文摘Light is a critical environmental cue that regulates a variety of diverse plant developmental processes.Cryptochrome 1(CRY1)is the major photoreceptor that mediates blue light-dependent photomorphogenic responses such as the inhibition of hypocotyl elongation.Gibberellin(GA)participates in the repression of photomorphogenesis and promotes hypocotyl elongation.However,the antagonistic interaction between blue light and GA is not well understood.Here,we report that blue light represses GA-induced degradation of the DELLA proteins(DELLAs),which are key negative regulators in the GA signaling pathway,via CRY1,thereby inhibiting the GA response during hypocotyl elongation.Both in vitro and in vivo biochemical analyses demonstrated that CRY1 physically interacts with GA receptors-GA-INSENSITIVE DWARF 1 proteins(GID1s)-and DELLAs in a blue light-dependent manner.Furthermore,we showed that CRY1 inhibits the association between GID1s and DELLAs.Genetically,CRY1 antagonizes the function of GID1s to repress the expression of cell elongation-related genes and thus hypocotyl elongation.Taken together,our findings demonstrate that CRY1 coordinates blue light and GA signali ng for plant photomorphogenesis by stabilizing DELLAs through the binding and in activation of GID1s,providing new in sights into the mechanism by which blue light antagonizes the function of GA in photomorphogenesis. | Ming Zhong Bingjie Zeng Dongying Tang Jiaxin Yang Lina Qu Jindong Yan Xiaochuan Wang Xin Li Xuanming Liu Xiaoying Zhao | 2021 | Molecular Plant2021，14，8： | 3 |
| 8 | Profile of the culturable microbiome capable of producing acyl-homoserine lactone in the tobacco phyllosphere显示文摘Bacterial populations coexisting in the phyllosphere niche have important effects on plant health. Quorum sensing (QS) allows bacteria to communicate via diffusible signal molecules, but QS-dependent behaviors in phyllosphere bacterial populations are poorly understood. We investigate the dense and diverse N-acyl-homoserine lactone (AHL)-producing phyllosphere bacteria living on tobacco leaf surfaces via a culture-dependent method and 16S rRNA gene sequencing. Our results indicated that approximately 7.9%-11.7% of the culturable leaf-associated bacteria have the ability to produce AHL based on the assays using whole-cell biosensors. Sequencing of the 16S rRNA gene assigned the AHL-producing strains to two phylogenetic groups, with Gammaproteobacteria (93%) as the predominant group, followed by Alphaproteobacteria. All of the AHL-producing Alphaproteobacteria were affiliated with the genus Rhizobium, whereas the AHL-producing bacteria belonging to the Gammaproteobacteria mainly fell within the genera Pseudomonas, Acinetobacter, Citrobacter, Enterobacter, Pantoea and Serratia. The bioassays of supernatant extracts revealed that a portion of the strains have a remarkable AHL profile for AHL induction activity using the two different biosensors, and one compound in the active extract of a representative isolate, NTL223, corresponded to 3-oxo-hexanoyl-homoserine lactone. A large population size and diversity of bacteria capable of AHL-driven QS were found to cohabit on leaves, implying that cross-communication based AHL-type QS may be common in the phyllosphere. Furthermore, this study provides a general snapshot of a potential valuable application of AHL-producing bacteria inhabiting leaves for their presumable ecological roles in the phyllosphere. | Di Lv Anzhou Ma Xuanming Tang Zhihui Bai Hongyan Qi Guoqiang Zhuang | 2013 | Journal of Environmental Sciences2013，25，2： | 3 |
| 9 | Protein identification and mRNA analysis of phyto-chrome-regulated genes in Arabidopsis under red light显示文摘Phytochromes are a family of plant photoreceptors that mediate physiological and developmental re- sponses to red and far-red light. According to the affymetrix ATH1 microarray, phytochrome A (phyA) and phytochrome B (phyB) together play a key role in transducing the Rc signals to light-responsive genes. In order to select those red light-responsive genes associated with phyA or phyB, a proteomic approach based on two-dimensional gel electrophoresis (2-DE) was used to compare the protein ex- pression patterns of the phyAphyB double mutant and the wild type of Arabidopsis thaliana (col-4) which grew under constant red light conditions for 7 d. Thirty-two protein spots which exhibited dif- ferences in protein abundance were identified by matrix-assisted laser desorption/ionization-time of flight/time of flight mass spectrometry. The expression of ten genes corresponding to ten protein spots was analyzed by a semiquantitative reverse transcription-polymerase chain reaction. Two of the ten genes were confirmed by quantitative PCR (Q-PCR). The results showed that phytochromes may exert their function by regulating mRNA or protein expressions. Proteomic analysis may provide a novel pathway for identifying phytochrome-dependent genes. | LI Xu*, YANG YueJun*, LI Yan, WANG Jie, XIAO XiaoJuan, GUO XinHong, TANG DongYing & LIU XuanMing Bioenergy and Biomaterial Research Center, College of Life Science and Biotechnology, Hunan University, Changsha 410082, China. | 2009 | Science China(Life Sciences)2009，52，4： | 2 |
| 10 | Troglitazone inhibits cell proliferation by attenuation of epidermal growth factor receptor signaling independent of peroxisome proliferator-activated receptor γ显示文摘Peroxisome 激活 proliferator 的受体(PPAR ) 属于 ligand 依赖的抄写因素的原子荷尔蒙受体总科。最近的结果证明了 PPAR 纬的收缩筋例如 troglitazone (TGZ ) ,能禁止房间增长并且支持独立于 PPAR 纬的房间区别。在现在的学习,我们提供 TGZ 可以直接绑在 EGFR 并且触发它发信号和 PPAR 纬的成为主观独立人士的证据。详细研究表明有 TGZ 的延长孵化有效地稀释了由指向受体到 endo-lysosomal 降级机械发信号的 EGFR。尽管细胞外的调整信号的发信号 kinase 小径被 TGZ 短暂地在 EGFR overexpressing 癌症细胞激活,导致 EGF 的 Akt phosphorylation 的抑制很可能说明了 TGZ 在 pharmacologically 可完成的集中引起的肿瘤细胞的生长拘捕。因此,我们提供了显示 TGZ 由支持 EGFR 降级和 attenuating Akt phosphorylation 禁止房间增长的证据的一根新线。 | Xiaoqi Li Xuanming Yang Youli Xu Xuejun Jiang Xin Li Fajun Nan Hong Tang | 2009 | Cell Research2009，19，6： | 2 |
| 11 | Comparative proteomics analysis of light responses in cryptochromel-304 and Columbia wild-type 4 of Arabidopsis thaliana显示文摘 | Yuejun Yang Yan Li Xu Li Xinhong Guo Xiaojuan Xiao Dongying Tang Xuanming Liu | 2008 | Acta Biochimica et Biophysica Sinica2008，40，1： | 2 |
| 12 | Do adaptive immune cells suppress or activate innate immunity?显示文摘 | Jie Zhao Xuanming Yang Sogyong L. Auh Kwang Dong Kim Hong Tang Yang-Xin Fu | 2008 | Trends in Immunology2008，，1： | 1 |
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