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| 1 | Homology-mediated end joining-based targeted integration using CRISPR/Cas9显示文摘 | Yao, Xuan Wang, Xing Hu, Xinde Liu, Zhen Liu, Junlai Zhou, Haibo Shen, Xiaowen Wei, Yu Huang, Zijian Ying, Wenqin Wang, Yan Nie, Yan-Hong Zhang, Chen-Chen Li, Sanlan Cheng, Leping Wang, Qifang Wu, Yan Huang, Pengyu Sun, Qiang Shi, Linyu Yang, Hui | 2017 | Cell Research2017，27，6： | 28 |
| 2 | One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs显示文摘CRISPR/Cas9 系统是一个有效编辑基因的方法,但是编辑基因的动物的多数显示出 mosaicism,与编辑仅仅在房间的部分发生。这里,我们证明那单个基因或多重基因能被 Cas9 mRNA 和多重邻近的单个指南的 RNA 的 zygotic 注射完全在老鼠和猴子胚胎击倒(spaced 10-200 bp 分开) 指向仅仅一把单个钥匙每基因的 exon。个别地在 Y 染色体上跟随八基因的指向的删除的 F0 老鼠的 Phenotypic 分析在产生猛烈老鼠表明了这条途径的坚韧性。重要地,这条途径在高效率交付完全的基因大美人(Arntl 上的 100% 并且 91% 在 Prrt2 上) 在猴子胚胎。最后,我们能在一个单个步骤产生一只完全的 Prrt2 猛烈猴子,表明在很快建立编辑基因的猴子模型的这条途径的实用性。 | Erwei Zuo Yi-Jun Cai Kui Li Yu Wei Bang-An Wang Yidi Sun Zhen Liu Jiwei Liu Xinde Hu Wei Wei Xiaona Huo Linyu Shi Cheng Tang Dan Liang Yan Wang Yan-Hong Nie Chen-Chen Zhang Xuan Yao Xing Wang Changyang Zhou Wenqin Ying Qifang Wang Ren-Chao Chen Qi Shen Guo-Liang Xu Jinsong Li Qiang Sun Zhi-Qi Xiong Hui Yang | 2017 | Cell Research2017，27，7： | 21 |
| 3 | Base editing-mediated splicing correction therapy for spinal muscular atrophy显示文摘Dear Editor,Spinal muscular atrophy(SMA)is a devastating autosomal recessive motor neuron disease.1,2 Infants with more severe forms of type I SMA die before the age of 2 if no intervention is provided.1,2 Spinraza and zolgensma have been approved by the FDA as SMA therapeutics for pediatric patients.1,2 However,as an antisense oligonucleotide(ASO)based therapy,spinraza requires four loading doses,followed by three annual maintenance doses.The patients would be subjected to repeated intrathecal injections in the procedure.Zolgensma is a single-dose gene-replacement therapy for SMA,but unfortunately is unreliable in maintaining a high,stable level of gene expression.These issues have limited the therapeutic effects of the two approved drugs for SMA. | Xiang Lin Haizhu Chen Ying-Qian Lu Shunyan Hong Xinde Hu Yanxia Gao Lu-Lu Lai Jin-Jing Li Zishuai Wang Wenqin Ying Lixiang Ma Ning Wang Erwei Zuo Hui Yang Wan-Jin Chen | 2020 | Cell Research2020，30，6： | 5 |
| 4 | Nuclear reprogramming: the zygotic transcription program is established through an“erase-and-rebuild” strategy显示文摘卵母细胞显示母亲特定的基因表达式侧面,它被换到当染色质的一个单倍体集合被传递到发展成一个胚胎的受精卵时, zygotic 介绍。位于这抄写 reprogramming 下面的机制当前是未知的。等到当抄写在幼芽的泡卵母细胞被关掉时,一般抄写因素和 transcriptional 管理者的一个范围从染色质被分裂,这里,我们表明那。染色质因素(CF ) 的全球分离破坏在染色质和 CF 之间的物理接触并且在功能的水平导致母亲的抄写节目的檫除。与染色质论坛分开的批评抄写因素和管理者遗体延长了时期,并且立即在专业版以后与染色质成为 re-associated 原子形成。Thisis 由象 DNA 和抄写那样的原子功能的重建跟随了速度集合。我们建议母亲的抄写节目在卵子发生期间被擦掉产生相对天真的染色质, zygotic 抄写节目是在授精以后的重建的重新。这个过程作为“ erase-and-rebuild ”被称为过程,它被用来重设抄写节目,和最可能的其它原子过程也,从母亲的到胚胎的。我们进一步在伴随的纸出现(高 T,等,房间物件 2007;17:135-150 .) 一样的策略也被采用到改编 transcriptional,这在体细胞介绍原子转移和单性生殖,建议这当模特儿在早胚胎开始期间对 transcriptional reprogramming 的所有形式普遍适用。从染色质的 CF 的排水量也为抄写沉默的现象提供解释在期间对 zygotic 转变母亲。 | Feng Sun Haiyan Fang Ruizhen Li Tianlong Gao Junke Zheng Xuejin Chen Wenqin Ying Hui Z Sheng | 2007 | Cell Research2007，17，2： | 5 |
| 5 | Indiscriminate ssDNA cleavage activity of CRISPR-Cas12a induces no detectable off-target effects in mouse embryos显示文摘Dear Editor,Newly discovered characteristics like'collateral effect'or trans-cleavage in CRISPR-Cas13 and CRISPR-Cas12 systems have enabled their usage in nucleic acid detection(Gootenberg et al.2017,2018;Chen et al.2018).The collateral RNA cleavage of Cas13a has been reported to be harmful for cell development(Wang et al.2019;Buchman et al.2020).As a representative gene editor of CRISPR-Cas12 system,CRISPR-Cas12a(Cpf1)holds great potential for therapeutic applications in the future(Zetsche et al.2015;Koo et al.2018;Campa et al.2019). | Yu Wei Yingsi Zhou Yajing Liu Wenqin Ying Ruiming Lv Qimeng Zhao Haibo Zhou Erwei Zuo Yidi Sun Hui Yang Changyang Zhou | 2021 | Protein & Cell2021，12，9： | 2 |
| 6 | Disruption of splicing-regulatory elements using CRISPR/Cas9 to rescue spinal muscular atrophy in human iPSCs and mice显示文摘We here report a genome-editing strategy to correct spinal muscular atrophy(SMA).Rather than directly targeting the pathogenic exonic mutations,our strategy employed Cas9 and guide-sg RNA for the targeted disruption of intronic splicing-regulatory elements.We disrupted intronic splicing silencers(ISSs,including ISS-N1 and ISS+100)of survival motor neuron(SMN)2,a key modifier gene of SMA,to enhance exon 7 inclusion and full-length SMN expression in SMA iPSCs.Survival of splicing-corrected iPSC-derived motor neurons was rescued with SMN restoration.Furthermore,co-injection of Cas9 mRNA from Streptococcus pyogenes(SpCas9)or Cas9 from Staphylococcus aureus(SaCas9)alongside their corresponding sgRNAs targeting ISS-N1 into zygotes rescued 56%and 100%of severe SMA transgenic mice(Smn-/-,SMN2tg/-).The median survival of the resulting mice was extended to>400 days.Collectively,our study provides proof-of-principle for a new strategy to therapeutically intervene in SMA and other RNA-splicing-related diseases. | Jin-Jing Li Xiang Lin Cheng Tang Ying-Qian Lu Xinde Hu Erwei Zuo He Li Wenqin Ying Yidi Sun Lu-Lu Lai Hai-Zhu Chen Xin-Xin Guo Qi-Jie Zhang Shuang Wu Changyang Zhou Xiaowen Shen Qifang Wang Min-Ting Lin Li-Xiang Ma Ning Wang Adrian R.Krainer Linyu Shi Hui Yang Wan-Jin Chen | 2020 | National Science Review2020，7，1： | 1 |
| 7 | The heterogeneity and dynamic equilibrium of rat embryonic stem cells显示文摘 | Yan Shen Cheng Shi Wei Wei Weidong Yu Wenlin Li Yang Yang Jun Xu Wenqin Ying Xin Sui Lingling Fang Weiwei Lin Huan Yang Sheng Ding Huan Shen Yan Shi Hongkui Deng | 2011 | Cell Research2011，21，7： | 1 |
| 8 | Tild-CRISPR可在小鼠和人类细胞中实现高效、精确的基因敲入显示文摘文章简介研究团队基于CRISPR/Cas9系统,设计了一种新的靶向整合策略Tild-CRISPR,通过PCR扩增或者精确酶切,获得含有800bp同源臂的转基因供体与Cas9 mRNA以及single-guide RNA一起注射到小鼠受精卵中。 | 姚璇 Meiling Zhang Xing Wang Wenqin Ying Xinde Hu Pengfei Dai Feilong Meng Linyu Shi Yun Sun Ning Yao Wanxia Zhong Yun Li Keliang Wu 李卫平 陈子江 杨辉 | 2019 | 科学新闻2019，0，2： | 0 |
| 9 | 共担与赋能:有温度的社区与住房显示文摘Introduction On Apr.15-16,2023,the 2022 Annual Conference of the Housing and Community Planning Committee of the Urban Planning Society of China(UPSC)was successfully held at the Chongqing Planning Exhibition Gallery,Chongqing.The conference was hosted by the Housing and Community Planning Committee of the UPSC. | 尹稚 王宇 江维 Yu Ying Liu Jiayan Yao Nan Wang Chenghui Fang Xiaotao Wang Ying Xi Wenqin Pan Chenyue Mou Yanchuan Wei Wei Li Yun Xiao Xiao Chen Yulin Zhao Di Liu Wei Zhang Jiuhe Deng Yi | 2023 | China City Planning Review2023，32，4： | 0 |
| 10 | Factors influencing success rate of IVF/ET:analysis of 770 treatment cycles显示文摘Objective: To evaluate the factors influencing success ra te of in vitro fertilization /embryo transfer(IVF/ET).Methods: A retrospective study of 770 consecutive cycles under gone IVF/ET or intracytoplasmic sperm injection (ICSI) from March 1999 to June 2 001.Estradiol and progesterone concentrations on the day of hCG administration w ere measured. Factors effecting embryo transfer on the effects of clinical pregn ancy rate were evaluated.Results: The overall clinical pregnancy rate in 770 cycles was 40.8%. Take home baby rate was 31%. No significantly differences rate were obse rved between groups with different estradiol levels.If the progesterone concentr ation on the day of hCG administration was >6.36 nmol/L, the implantation and cl inical pregnancy rate was lower(P<0.003). The lowest clinical pregnancy rat e was observed when the duration of infertility was ≥10 years. The average numb er of embryos transferred was 2.23±0.83.Conclusion: This study demonstrated that the most important fac tor on clinical pregnancy rate was low progesterone concentration on the day of hCG administration. The other two important variables influencing success rate o f IVF/ET were the duration of infertility, the number of high-grade embryos tra nsferred. | Ye Bilu(叶碧绿) Lin Jinju(林金菊) Zhou Ying(周颖) Huang Xuefeng(黄学峰) Zhao Junzhao(赵军招) Zheng Jufen(郑菊芬) Lin Wenqin(林文琴) | 2002 | 生殖医学杂志2002，11，z1： | 0 |
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