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| 1 | Generation of a precise Oct4-hrGFP knockin cynomolgus monkey model via CRISPR/Cas9-assisted homologous recombination显示文摘 | Yiqiang Cui Yuyu Niu Jiankui Zhou Yongchang Chen Yiwei Cheng Shangang Li Zongyong Ai Chu Chu Hong Wang Bo Zheng Xuejin Chen Jiahao Sha Xuejiang Guo Xingxu Huang Weizhi Ji | 2018 | Cell Research2018，28，3： | 13 |
| 2 | Transgenic rhesus monkeys carrying the human MCPH1 gene copies show human-like neoteny of brain development显示文摘Brain size and cognitive skills are the most dramatically changed traits in humans during evolution and yet the genetic mechanisms underlying these human-specific changes remain elusive.Here,we successfully generated 11 transgenic rhesus monkeys(8 first-generation and 3 second-generation)carrying human copies of MCPH1,an important gene for brain development and brain evolution.Brain-image and tissue-section analyses indicated an altered pattern of neural-cell differentiation,resulting in a delayed neuronal maturation and neural-fiber myelination of the transgenic monkeys,similar to the known evolutionary change of developmental delay(neoteny)in humans.Further brain-transcriptome and tissue-section analyses of major developmental stages showed a marked human-like expression delay of neuron differentiation and synaptic-signaling genes,providing a molecular explanation for the observed brain-developmental delay of the transgenic monkeys.More importantly,the transgenic monkeys exhibited better short-term memory and shorter reaction time compared with the wild-type controls in the delayed-matching-to-sample task.The presented data represent the first attempt to experimentally interrogate the genetic basis of human brain origin using a transgenic monkey model and it values the use of non-human primates in understanding unique human traits. | Lei Shi Xin Luo Jin Jiang Yongchang chen Cirong Liu Ting Hu Min Li Qiang Lin Yanjiao Li Jun Huang Hong Wang Yuyu Niu Yundi Shi Martin Styner Jianhong Wang Yi Lu Xuejin Sun Hualin Yu Weizhi Ji Bing Su | 2019 | National Science Review2019，6，3： | 12 |
| 3 | Friction moment analysis of space gyroscope bearing with ribbon cage under ultra-low oscillatory motion显示文摘This paper presents the model of calculating the total friction moment of space gyroscope ball bearings which usually work under ultra-low oscillatory motion and are very sensitive to the friction moment. The aim is to know the proportion of the friction moment caused by each frictional source in the bearing's total friction moment, which is helpful to optimize the bearing design to deduce the friction moment. In the model, the cage dynamic equations considering six degree-of-freedom and the balls dynamic equations considering two degree-of-freedom were solved.The good trends with different loads between the measured friction moments and computational results prove that the model under constant rate was validated. The computational results show that when the speed was set at 5 r/min, the bearing's maximum total friction moment when oscillation occurred was obviously larger than that occurred at a constant rate. At the onset of each oscillatory motion, the proportion of the friction moment caused by cage in the bearing's total friction moment was very high, and it increased with the increasing speed. The analyses of different cage thicknesses and different clearances between cage pocket and ball show that smaller thickness and clearance were preferred. | Jiang Shaona Chen Xiaoyang Gu Jiaming Shen Xuejin | 2014 | Chinese Journal of Aeronautics2014，27，5： | 5 |
| 4 | Nuclear reprogramming: the zygotic transcription program is established through an“erase-and-rebuild” strategy显示文摘卵母细胞显示母亲特定的基因表达式侧面,它被换到当染色质的一个单倍体集合被传递到发展成一个胚胎的受精卵时, zygotic 介绍。位于这抄写 reprogramming 下面的机制当前是未知的。等到当抄写在幼芽的泡卵母细胞被关掉时,一般抄写因素和 transcriptional 管理者的一个范围从染色质被分裂,这里,我们表明那。染色质因素(CF ) 的全球分离破坏在染色质和 CF 之间的物理接触并且在功能的水平导致母亲的抄写节目的檫除。与染色质论坛分开的批评抄写因素和管理者遗体延长了时期,并且立即在专业版以后与染色质成为 re-associated 原子形成。Thisis 由象 DNA 和抄写那样的原子功能的重建跟随了速度集合。我们建议母亲的抄写节目在卵子发生期间被擦掉产生相对天真的染色质, zygotic 抄写节目是在授精以后的重建的重新。这个过程作为“ erase-and-rebuild ”被称为过程,它被用来重设抄写节目,和最可能的其它原子过程也,从母亲的到胚胎的。我们进一步在伴随的纸出现(高 T,等,房间物件 2007;17:135-150 .) 一样的策略也被采用到改编 transcriptional,这在体细胞介绍原子转移和单性生殖,建议这当模特儿在早胚胎开始期间对 transcriptional reprogramming 的所有形式普遍适用。从染色质的 CF 的排水量也为抄写沉默的现象提供解释在期间对 zygotic 转变母亲。 | Feng Sun Haiyan Fang Ruizhen Li Tianlong Gao Junke Zheng Xuejin Chen Wenqin Ying Hui Z Sheng | 2007 | Cell Research2007，17，2： | 5 |
| 5 | THE MANAGEMENT AND RESEARCH OF GEOGRAPHICAL NAMES USING GEOGRAPHICAL INFORMATION SYSTEM显示文摘Based on the combination of Geographical information system(GIS)with the research ofToponymy,some foundational problems of the Geographical Name Information System(GINS)are discussed in this paper.The present situation of Toponymical research is anal-ysed and the necessity of introducing the GIS tools to the research of Toponymy is demon-strated.The conceptual model of the GNIS and the structure of Geographical name Database(GND)are discussed.The basic functions of the GNIS software for management and re-search of geographical name are suggested.Some issues are stated such as the applicationand potential of the combination of GIS with CAD in the management and research of To-ponymy,the combination of GNIS with other spatial information system and sharing the in-formation with each other,the research and development of GNIS, etc. | Yu Guangming Chen Ping Wu Xuejin(Department of Geography) | 1992 | 湖北科技学院学报1992，23，3： | 4 |
| 6 | Genetic basis of high aroma and stress toleranee in the oolong tea cultivar genome显示文摘Tea plants(Camellia sinensis)are commercially cultivated in>60 countries,and their fresh leaves are processed into tea,which is the most widely consumed beverage in the world.Although several chromosome-level tea plant genomes have been published,they collapsed the two haplotypes and ignored a large number of allelic variations that may underlie important biological functions in this species.Here,we present a phased chromosome-scale assembly for an elite oolong tea cultivar,'Huangdan',that is well known for its high levels of aroma.Based on the two sets of haplotype genome data,we identi fi ed numerous genetic variations and a substantial proportion of allelic imbalance related to important traits,including aroma-and stress-related alleles.Comparative genomics revealed extensive structural variations as well as expansion of some gene families,such as terpene synthases(TPSs),that likely contribute to the high-aroma characteristics of the backbone parent,underlying the molecular basis for the biosynthesis of aroma-related chemicals in oolong tea.Our results uncovered the genetic basis of special features of this oolong tea cultivar,providing fundamental genomic resources to study evolution and domestication for the economically important tea crop. | Pengjie Wang Jiaxin Yu Shan Jin Shuai Chen Chuan Yue Wenling Wang Shuilian Gao Hongli Cao Yucheng Zheng Mengya Gu Xuejin Chen Yun Sun Yuqiong Guo Jiangfan Yang Xingtan Zhang Naixing Ye | 2021 | Horticulture Research2021，8，1： | 4 |
| 7 | Identification of PAL genes related to anthocyanin synthesis in tea plants and its correlation with anthocyanin content显示文摘The phenylalanine ammonia-lyase(PAL)gene family in tea plants(Camellia sinensis L.)encodes the enzyme that catalyzes the first reaction of the phenylpropane metabolic pathway.The present study aimed to characterize the PAL genes in tea plants,and get better insights on the CsPALs in anthocyanins accumulation.Seven CsPAL genes were identified and characterized in tea plants by bioinformatics analysis.Systematic analysis of CsPALs was conducted for its phylogenetic relationship,gene structure,chromosomal location,and protein conserved motifs based on tea plant genome.The cis-elements of CsPALs were responsive to light,abiotic stress,hormone,and MYB-binding site.Furthermore,tissuespecific expression analysis showed that CsPAL4 was expressed preferentially in young leaves and buds.Correlation analysis was performed in purple-leaf tea with anthocyanin components,and it was suggested that CsPAL4 was closely related with different anthocyanin accumulated,especially with cyanidin 3-O-galactoside,cyanidin 3-O-glucoside,and delphinidin 3-O-glucoside.Additionally,the putative upstream regulation factors CsMYBs(CsMYB59,CsARR1,CsSRM1,CsMYB101,and CsMYB52)and CsbHLHs(CsbHLH104,CsbHLH3,CsbIM1,CsTCP14,and CsPIF4)could bind to the promoter of CsPALs,thereby activating its transcription.This study provides a theoretical basis for further research to elucidate the functions of the CsPAL genes. | Xuejin Chen Pengjie Wang Mengya Gu Binghao Hou Churan Zhang Yucheng Zheng Yun Sun Shan Jin Naixing Ye | 2022 | Horticultural Plant Journal2022，8，3： | 4 |
| 8 | Conversion of female germline stem cells from neonatal and prepubertal mice into pluripotent stern cells显示文摘 | Hu Wang Manxi Jiang Haiwei Bi Xuejin Chen Lin He Xiaoyong Li Ji Wu | 2014 | Journal of Molecular Cell Biology2014，8，2： | 3 |
| 9 | Experimental investigation of EHD grease lubrication in finite line contacts显示文摘The objective of this study was to investigate the grease-lubricated film-forming mechanisms in the finite line contact and to improve the grease-lubricated finite line contact’s film-forming capacity.An elastohydrodynamic lubrication(EHL) test rig with two interferometry microscopes,which could simultaneously monitor two different contact locations in the finite line contact,was constructed in order to study the influences of the grease thickener formulation on the film thickness and lubrication condition.By using the relative light intensity method,the thickness maps of the grease-lubricated film were calculated from the interferometer images captured by the two microscopes.The test results revealed that the grease thickener’s formulation had remarkable effects on film formation and the perturbation of film thickness.For the lithium-based grease,the film’s thickness near the two ends of the roller was prone to severe perturbation caused by the conglomeration of clumps that were hard to shear.For the aluminum-complex-based grease,the fibers tended to accumulate in the middle of the roller rather than at the two ends.The urea-based grease could be easily sheared into smaller particles.In addition to the straight-line profile rollers,the logarithmic profile rollers were tested and found to effectively enhance the axial grease flow,increase the axial shear stress,and thus shear more fibers into particles within the contact area. | Zhijian WANG Xuejin SHEN Xiaoyang CHEN Dehua TAO Lei SHI Shuangbiao LIU | 2019 | Friction2019，7，3： | 2 |
| 10 | ELASTOHYDRODYNAMIC LUBRICATION OF LOGARITHMIC PROFILE ROLLER CONTACTS显示文摘An account of numerical solutions to the isothermal and flooded elastohydrodynamic lubrication(EHL)of a logarithmic profile roller,which is rolling over a flat plane,is given The analysis takes account of sideways flow of lubricant in the inlet region of the contact When the results are presented in suitable non dimensional groups,it is shown that more uniformly pressure and shape of the film distributing in axial direction is taken place under light loading As the increase of the load,the end closure is displayed and the oil pressure rises sharply at the ends The seal action formed by the end closure makes the film thickness a little And the minimum film thickness is transferred from the central to the ends and the value is reduced rapidly As the increase of the speed,the end closure becomes much serious The optimum crowning value obtained in EHL state is larger than the design value obtained in elastostatic contact state for the same working conditions In order to verify the correctness of theory,optical interferometry is applied to measure the oil film thickness between a logarithmic profiled roller and a glass plate under pure rolling conditions It is found the agreement between numerical solutions and experiments is very good. | Chen Xiaoyang, Shen Xuejin (Research Institute of Bearings,Shanghai University) Xu Wen, Ma Jiaju (Zhejiang University) | 2001 | Chinese Journal of Mechanical Engineering2001，14，4： | 2 |
| 11 | Myeloid-derived suppressor cells as immunosuppressive regulators and therapeutic targets in cancer显示文摘Myeloid-derived suppressor cells(MDSCs)are a heterogenic population of immature myeloid cells with immunosuppressive effects,which undergo massive expansion during tumor progression.These cells not only support immune escape directly but also promote tumor invasion via various non-immunological activities.Besides,this group of cells are proved to impair the efficiency of current antitumor strategies such as chemotherapy,radiotherapy,and immunotherapy.Therefore,MDSCs are considered as potential therapeutic targets for cancer therapy.Treatment strategies targeting MDSCs have shown promising outcomes in both preclinical studies and clinical trials when administrated alone,or in combination with other anticancer therapies.In this review,we shed new light on recent advances in the biological characteristics and immunosuppressive functions of MDSCs.We also hope to propose an overview of current MDSCs-targeting therapies so as to provide new ideas for cancer treatment. | Kai Li Houhui Shi Benxia Zhang Xuejin Ou Qizhi Ma Yue Chen Pei Shu Dan Li Yongsheng Wang | 2021 | Signal Transduction and Targeted Therapy2021，6，11： | 2 |
| 12 | Noggin versus basic fibroblast growth factor on the differentiation of human embryonic stem cells显示文摘The difference between Noggin and basic fibroblast growth factor for the neural precursor differen-tiation from human embryonic stem cells has not been studied. In this study, 100 μg/L Noggin or 20 μg/L basic fibroblast growth factor in serum-free neural induction medium was used to differen-tiate human embryonic stem cells H14 into neural precursors using monolayer differentiation. Two weeks after induction, significantly higher numbers of neural rosettes formed in the Noggin-induced group than the basic fibroblast growth factor-induced group, as detected by phase contrast micro-scope. Immunofluorescence staining revealed expression levels of Nestin, β-III Tubulin and Sox-1 were higher in the induced cells and reverse-transcription PCR showed induced cells expressed Nestin, Sox-1 and Neurofilament mRNA. Protein and mRNA expression in the Noggin-induced group was increased compared with the basic fibroblast growth factor-induced group. Noggin has a greater effect than basic fibroblast growth factor on the induction of human embryonic stem cell differentiation into neural precursors by monolayer differentiation, as Noggin accelerates and in-creases the differentiation of neural precursors. | Yan Zhang Junmei Zhou Zhenfu Fang Manxi Jiang Xuejin Chen | 2013 | Neural Regeneration Research2013，8，23： | 2 |
| 13 | Cloning of Rabbit HPRT Gene Using the Recombineering System显示文摘次黄嘌呤 phosphoribosyltransferase ( HPRT )在嘌呤的新陈代谢的抢救起一个重要作用,并且在 manystudies.To 为变异的选择用作一条其他的小径在兔子便于它的申请,我们用联合的一条途径克隆 cDNA 和兔子 HPRT 基因的 genomic DNA 生物信息学并且重新结合 ering 方法。由为 218 氨基酸的蛋白质包含一个编码序列的 1449 bp 组成的 cDNAis。兔子 HPRT 基因的推出的氨基酸顺序与人,老鼠,猪和牛 HPRT 基因分享 98%,97%,98% 和 94% 身份, respectively.Reverse 抄写聚合酶链反应分析证明这基因无所不在地在成年兔子的纸巾被表示。兔子 HPRT 基因在长度跨越约 48 kb 并且由在克隆大尺寸的基因兔子 HPRT 基因表演克隆 recombine ering 系统的实用性的九 exons.The 组成。这个系统可以便于为克隆大尺寸的基因或在定序的 genomic 充满大差距从细菌的人工的染色体 DNA 克隆的潜水艇。 | Jianjun SHI Donghui CAI Xuejin CHEN Huizheng SHENG | 2007 | Acta Biochimica et Biophysica Sinica2007，39，8： | 2 |
| 14 | Chromatin accessibility and translational Iandscapes of tea plants under chilling stress显示文摘Plants have evolved regulatory mechanisms at multiple levels to regulate gene expression in order to improve their cold adaptability.However,limited information is available regarding the stress response at the chromatin and translational levels.Here,we characterize the chromatin accessibility,transcriptional,and translational landscapes of tea plants in vivo under chilling stress for the first time.Chilling stress signi ficantly affected both the transcription and translation levels as well as the translation efficiency of tea plants.A total of 3010 genes that underwent rapid and independent translation under chilling stress were observed,and they were signi ficantly enriched in the photosynthesis-antenna protein and phenylpropanoid biosynthesis pathways.A set of genes that were signi ficantly responsive to cold at the transcription and translation levels,including four(+)-neomenthol dehydrogenases(MNDs)and two(E)-nerolidol synthases(NESs)arranged in tandem on the chromosomes,were also found.We detected potential upstream open reading frames(uORFs)on 3082 genes and found that tea plants may inhibit the overall expression of genes by enhancing the translation of uORFs under chilling stress.In addition,we identi fied distal transposase hypersensitive sites(THSs)and proximal THSs and constructed a transcriptional regulatory network for tea plants under chilling stress.We also identi fied 13 high-con fidence transcription factors(TFs)that may play a crucial role in cold regulation.These results provide valuable information regarding the potential transcriptional regulatory network in plants and help to clarify how plants exhibit flexible responses to chilling stress. | Pengjie Wang Shan Jin Xuejin Chen Liangyu Wu Yucheng Zheng Chuan Yue Yongchun Guo Xingtan Zhang Jiangfan Yang Naixing Ye | 2021 | Horticulture Research2021，8，1： | 2 |
| 15 | Extreme points and rotundity of Orlicz-Sobolev spaces显示文摘 | Chen Shutao Hu Changying Charles Xuejin Zhao | 2002 | Inter J Math Math Sci2002，32，5： | 1 |
| 16 | Uniform rotundity of Orlicz-Sobolev spaces显示文摘 | Chen Shutao Hu Changying Charles Xuejin Zhao | 2003 | Soochow J Math2003，29，3： | 1 |
| 17 | Cloud Classification Based on Structure Features of Infrared Images显示文摘 | Liu Lei Sun Xuejin Chen Feng Zhao Shijun Gao Taichang | 2011 | Journal of Atmospheric and Oceanic Technology2011，，3： | 1 |
| 18 | Extreme points and rotundity of orlicz-sobolev space显示文摘 | Chen Shutao Hu Changing Zhan Xuejin | 2002 | Inter J Math Sci2002，32，5： | 1 |
| 19 | Clinical application of tenloriun cerebelli hiatus incision treatment of severe and most severe brain injuries显示文摘 | Wang Yuhai Chen Zheng li | 2001 | Chinese Traunmatalogy2001，4，： | 1 |
| 20 | Cloud classi- fication based on structure features of infrared images 显示文摘 | LIU Lei SUN Xuejin CHEN Feng | 2011 | Journal of Atmospheric and Oceanic Technology2011，28，3： | 1 |
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