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| 1 | Standardized Operational Protocol for Human Brain Banking in China显示文摘With the progress of society, there is an increasing need to tackle disorders of the central nervous system. Human brain tissue, unlike animal tissues, is an irreplaceable resource for the study of neurological diseases (1)Aimed at scientific research and education, the roles of human brain tissue repositories are to acquire brain tissue from donors, prepare, process, and preserve collected samples,provide tissue to specific eligible facilities, and determine the characteristics of each tissue sample. | Wenying Qiu Hanlin Zhang Aimin Bao Keqing Zhu Yue Huang Xiaoxin Yan Jing Zhang Chunjiu Zhong Yong Shen Jiangning Zhou Xiaoying Zheng Liwei Zhang Yousheng Shu Beisha Tang Zhenxin Zhang Gang Wang Ren Zhou Bing Sun Changlin Gong Shumin Duan Chao Ma | 2019 | Neuroscience Bulletin2019，35，2： | 6 |
| 2 | Correlations Between Single Nucleotide Polymorphisms,Cognitive Dysfunction,and Postmortem Brain Pathology in Alzheimer's Disease Among Han Chinese显示文摘In this study, the distribution of five Alzheimer's disease(AD)-related single nucleotide polymorphisms(SNPs) in the Han population was examined in combination with the evaluation of clinical cognition and brain pathological analysis. The associations among SNPs,clinical daily cognitive states, and postmortem neuropathological changes were analyzed in 110 human brains from the Chinese Academy of Medical Sciences/Peking Union Medical College(CAMS/PUMC) Human Brain Bank.APOE ?4(OR = 4.482, P = 0.004), the RS2305421 GG genotype(adjusted OR = 4.397, P = 0.015), and the RS10498633 GT genotype(adjusted OR = 2.375,P = 0.028) were associated with a higher score on the ABC(Ab plaque score, Braak NFT stage, and CERAD neuritic plaque score) dementia scale. These results advance our understanding of the pathogenesis of AD,the relationship between pathological diagnosis and clinical diagnosis, and the SNPs in the Han population for future research. | Qian Yang Kang Chen Hanlin Zhang Wanying Zhang Changlin Gong Qing Zhang Pan Liu Tianyi Sun Yuanyuan Xu Xiaojing Qian Wenying Qiu Chao Ma | 2019 | Neuroscience Bulletin2019，35，2： | 5 |
| 3 | Expression of biologically active human clotting factor Ⅸ(hFⅨ) in the mammary gland of transgenic mice显示文摘The DNA of human factor Ⅸ (hFⅨ) gene vector pMCⅨm, which had been proven to be able to express in in vitro and living cells, was introduced into 586 zygotes of Kunming White Mice by positive pressure microinjection technique with manual operation. The 499 survival embryos after microinjection were then transferred into pseudopregnant recipient mice and 216 F 0 pups were born. The analysis of PCR and Southern blot hybridization showed that, of the 216, 6 (2 females and 4 males) were integrated with foreign DNA in their genomes, giving an integration frequency of 3% (6/216). Two F\-0 female transgenic mice could express hFⅨ protein in their milk and the content was over 100 ng/mL as measured with ELISA. The biological activities of hFⅨ in the milk of two F\-0 mice were 44 67% and 79 43%, respectively. | HUANG Ying 1, ZHANG Kezhong 2, HUANG Wenying 1, LU Daru 2, HUANG Ying 1, MA Zhanlu 1, REN Zhaorui 1, QIU Xinfang 2, XUE Jinglun 2, ZENG Yitao 1 and HUANG Shuzhen 1* 1. Shanghai Institute of Medical Genetics, Shanghai Children’s Hospita | 1998 | Chinese Science Bulletin1998，43，15： | 2 |
| 4 | Transcriptome dynamics of hippocampal neurogenesis in macaques across the lifespan and aged humans显示文摘Whether adult hippocampal neurogenesis(AHN)persists in adult and aged humans continues to be extensively debated.A major question is whether the markers identified in rodents are reliable enough to reveal new neurons and the neurogenic trajectory in primates.Here,to provide a better understanding of AHN in primates and to reveal more novel markers for distinct cell types,droplet-based single-nucleus RNA sequencing(snRNA-seq)is used to investigate the cellular heterogeneity and molecular characteristics of the hippocampi in macaques across the lifespan and in aged humans.All of the major cell types in the hippocampus and their expression profiles were identified.The dynamics of the neurogenic lineage was revealed and the diversity of astrocytes and microglia was delineated.In the neurogenic lineage,the regulatory continuum from adult neural stem cells(NSCs)to immature and mature granule cells was investigated.A group of primate-specific markers were identified.We validated ETNPPL as a primate-specific NSC marker and verified STMN1 and STMN2 as immature neuron markers in primates.Furthermore,we illustrate a cluster of active astrocytes and microglia exhibiting proinflammatory responses in aged samples.The interaction analysis and the comparative investigation on published datasets and ours imply that astrocytes provide signals inducing the proliferation,quiescence and inflammation of adult NSCs at different stages and that the proinflammatory status of astrocytes probably contributes to the decrease and variability of AHN in adults and elderly individuals. | Wei Wang Mengdi Wang Meng Yang Bo Zeng Wenying Qiu Qiang Ma Xiaoxi Jing Qianqian Zhang Bosong Wang Chonghai Yin Jiyao Zhang Yuxin Ge Yufeng Lu Weizhi Ji Qian Wu Chao Ma Xiaoqun Wang | 2022 | Cell Research2022，32，8： | 2 |
| 5 | Modulation of experimental osteoporosis in rats by the antioxidant beverage effective microorganism-X (EM-X)显示文摘 | Bin Ke Zenglu Xu Yunfei Ling Wenying Qiu Yuanyuan Xu Teruo Higa Okezie I. Aruoma | 2008 | Biomedicine & Pharmacotherapy2008，，2： | 1 |
| 6 | Isolation and identification of endophytic fungus SX01, a red pigment producer from Ginkgo biloba L 显示文摘 | Qiu M Xie RS ShiY ChenI!M WenY L GaoYS Hu X F | 2010 | World Journal of Microbiology & Biotechnology2010，26，6： | 1 |
| 7 | Brain Banks Spur New Frontiers in Neuropsychiatric Research and Strategies for Analysis and Validation显示文摘Neuropsychiatric disorders affect hundreds of millions of patients and families worldwide.To decode the molecular framework of these diseases,many studies use human postmortem brain samples.These studies reveal brain-specific genetic and epigenetic patterns via highthroughput sequencing technologies.Identifying best practices for the collection of postmortem brain samples,analyzing such large amounts of sequencing data,and interpreting these results are critical to advance neuropsychiatry.We provide an overview of human brain banks worldwide,including progress in China,highlighting some well-known projects using human postmortem brain samples to understand molecular regulation in both normal brains and those with neuropsychiatric disorders.Finally,we discuss future research strategies,as well as state-of-the-art statistical and experimental methods that are drawn upon brain bank resources to improve our understanding of the agents of neuropsychiatric disorders. | Le Wang Yan Xia Yu Chen Rujia Dai Wenying Qiu Qingtuan Meng Liz Kuney Chao Chen | 2019 | Genomics, Proteomics & Bioinformatics2019，17，4： | 1 |
| 8 | Comparison of Reference Genes for Transcriptional Studies in Postmortem Human Brain Tissue Under Different Conditions显示文摘Dear Editor Research on neurodegenerative diseases is a hot topic worldwide[1],and MRI,genetics and epigenetics,and animal models have been commonly used.Considering species differences,research on human brain samples is irreplaceable(2)Many countries and regions have built brain banks to collect and store brain tissue from donors.Samples provided by a brain bank can be used to study synaptic structure,protein,DNA,RNA[3,4],and lipids to illuminate the pathological mechanisms underlying neurodegenerative diseases.When brain tissue is used for gene expression research,the quantitative real-time polymerase chain reaction(RT-qPCR)is an accurate method,which simultaneously amplifies and quantifies the expression of target genes by measuring the intensity of fluorescence in each PCR cycle. | Qing Zhang Hanlin Zhang Fan Liu Qian Yang Kang Chen Pan Liu Tianyi Sun Chao Ma Wenying Qiu Xiaojing Qian | 2019 | Neuroscience Bulletin2019，35，2： | 0 |
| 9 | Plant genome editing:CRISPR,base editing,prime editing,and beyond显示文摘The clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)system is a fast-growing,genome editing technology that has wide applications in identifying gene functions as well as improving agricultural production and crop breeding.Here,we summarized recent advances in the development and applications of genome editing technologies in plants.We briefly described CRISPR/Cas9 technology and examined the base and prime editing techniques that have been developed from CRISPR technology.Some new prime editing-derived techniques were assessed. | Yujie Xie Syed Inzimam Ul Haq Xingyu Jiang Dianfeng Zheng Naijie Feng Wenying Wang Jin-Sheng He Quan-Sheng Qiu | 2022 | Grassland Research2022，1，4： | 0 |
| 10 | A Global Multiregional Proteomic Map of the Human Cerebral Cortex显示文摘The Brodmann area(BA)-based map is one of the most widely used cortical maps for studies of human brain functions and in clinical practice;however,the molecular architecture of BAs remains unknown.The present study provided a global multiregional proteomic map of the human cerebral cortex by analyzing 29 BAs.These 29 BAs were grouped into 6 clusters based on similarities in proteomic patterns:the motor and sensory cluster,vision cluster,auditory and Broca’s area cluster,Wernicke’s area cluster,cingulate cortex cluster,and heterogeneous function cluster.We identified 474 cluster-specific and 134 BA-specific signature proteins whose functions are closely associated with specialized functions and disease vulnerability of the corresponding cluster or BA.The findings of the present study could provide explanations for the functional connections between the anterior cingulate cortex and sensorimotor cortex and for anxiety-related function in the sensorimotor cortex.The brain transcriptome and proteome comparison indicates that they both could reflect the function of cerebral cortex,but show different characteristics.These proteomic data are publicly available at the Human Brain Proteome Atlas(www.brain-omics.com).Our results may enhance our understanding of the molecular basis of brain functions and provide an important resource to support human brain research. | Zhengguang Guo Chen Shao Yang Zhang Wenying Qiu Wenting Li Weimin Zhu Qian Yang Yin Huang Lili Pan Yuepan Dong Haidan Sun Xiaoping Xiao Wei Sun Chao Ma Liwei Zhang | 2022 | Genomics, Proteomics & Bioinformatics2022，20，4： | 0 |
| 11 | Focal-type,but not Diffuse-type,Amyloid Beta Plaques are Correlated with Alzheimer’s Neuropathology,Cognitive Dysfunction,and Neuroinflammation in the Human Hippocampus显示文摘Amyloid beta(Aβ)plaques are one of the hallmarks of Alzheimer’s disease(AD).However,currently available anti-amyloid therapies fail to show effectiveness in the treatment of AD in humans.It has been found that there are different types of Aβplaque(diffuse and focal types)in the postmortem human brain.In this study,we aimed to investigate the correlations among different types of Aβplaque and AD-related neuropathological and cognitive changes based on a postmortem human brain bank in China.The results indicated that focal plaques,but not diffuse plaques,significantly increased with age in the human hippocampus.We also found that the number of focal plaques was positively correlated with the severity of AD-related neuropathological changes(measured by the“ABC”scoring system)and cognitive decline(measured by the Everyday Cognitive Insider Questionnaire).Furthermore,most of the focal plaques were co-localized with neuritic plaques(identified by Bielschowsky silver staining)and accompanied by microglial and other inflammatory cells.Our findings suggest the potential of using focal-type but not general Aβplaques as biomarkers for the neuropathological evaluation of AD. | Fan Liu Jianru Sun Xue Wang Sixuan Jin Fengrun Sun Tao Wang Bo Yuan Wenying Qiu Chao Ma | 2022 | Neuroscience Bulletin2022，38，10： | 0 |
| 12 | Tracheal microenvironment,ANP metabolism and airway tone显示文摘Asthma is a chronic inflammatory disease,characterized by reversible airflow obstruction and airway hyperresponsiveness.There has been a sharp increase in the prevalence,morbidity,mortality and economic burden associated with this disorder over the last several decades,which triggers the development of various antiasthmatic agents[1].Maintenance of airway tone is the key in asthma therapy. | Qipu Wang Kuikui Jiang Wanying Zhang Wenying Qiu Yijia Li Yiqing Zheng Chen Wang Jimin Cao | 2016 | Science Bulletin2016，61，20： | 0 |
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