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| 1 | The Tea Tree Genome Provides Insights into Tea Flavor and Independent Evolution of Caffeine Biosynthesis显示文摘茶是世界有巨大的经济、药用、文化的重要性的最旧、很流行的包含咖啡因的饮料。这里,我们在场第一个高质量的核苷酸序列充满重复(80.9%) ,栽培的茶树山茶 sinensis 的 3.02-Gb 染色体。我们证明茶树的一种非常地大的染色体尺寸从一些 LTR retrotransposon 家庭的慢、稳定、长期的扩大被结果。除了一个最近的整个染色体的复制事件,基因的系特定的扩大把新陈代谢的生合成与 flavonoid 联系了被发现,它提高 catechin 生产,萜烯酶激活,和应力公差,为茶风味和改编的重要特征。我们相对可可子和咖啡表明一个独立人士和茶咖啡因合成小径的快速的进化。在 25 山茶种类之中的比较研究表明那更高的表情大多数 flavonoid- 和咖啡因铺平 -- 然而并非 theanine 相关的基因贡献 catechins 和咖啡因的增加的生产并且因此提高处理茶的适用性和茶质量。这些新奇调查结果为进一步的 metabolomic 和典型生合成小径的功能的 genomic 精炼铺平道路并且将帮助开发将最后满足并且吸引世界范围的更多的茶喝酒者的茶风味的一个更多样化的集合。 | En-Hua Xia Hai-Bin Zhang Jun Sheng Kui Li Qun-Jie Zhang Changhoon Kim Yun Zhang Yuan Liu Ting Zhu Wei Li Hui Huang Yan Tong Hong Nan Cong Shi Chao Shi Jian-Jun Jiang Shu-Yan Mao Jun-Ying Jiao Dan Zhang Yuan Zhao You-Jie Zhao Li-Ping Zhang Yun-Long Liu Ben-Ying Liu Yue Yu Sheng-Fu Shao De-Jiang Ni Evan E. Eichler Li-Zhi Gao | 2017 | Molecular Plant2017，10，6： | 113 |
| 2 | One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs显示文摘CRISPR/Cas9 系统是一个有效编辑基因的方法,但是编辑基因的动物的多数显示出 mosaicism,与编辑仅仅在房间的部分发生。这里,我们证明那单个基因或多重基因能被 Cas9 mRNA 和多重邻近的单个指南的 RNA 的 zygotic 注射完全在老鼠和猴子胚胎击倒(spaced 10-200 bp 分开) 指向仅仅一把单个钥匙每基因的 exon。个别地在 Y 染色体上跟随八基因的指向的删除的 F0 老鼠的 Phenotypic 分析在产生猛烈老鼠表明了这条途径的坚韧性。重要地,这条途径在高效率交付完全的基因大美人(Arntl 上的 100% 并且 91% 在 Prrt2 上) 在猴子胚胎。最后,我们能在一个单个步骤产生一只完全的 Prrt2 猛烈猴子,表明在很快建立编辑基因的猴子模型的这条途径的实用性。 | Erwei Zuo Yi-Jun Cai Kui Li Yu Wei Bang-An Wang Yidi Sun Zhen Liu Jiwei Liu Xinde Hu Wei Wei Xiaona Huo Linyu Shi Cheng Tang Dan Liang Yan Wang Yan-Hong Nie Chen-Chen Zhang Xuan Yao Xing Wang Changyang Zhou Wenqin Ying Qifang Wang Ren-Chao Chen Qi Shen Guo-Liang Xu Jinsong Li Qiang Sun Zhi-Qi Xiong Hui Yang | 2017 | Cell Research2017，27，7： | 21 |
| 3 | The Genome of Medicinal Plant Macleaya cordata Provides New Insights into Benzylisoquinoline Alkaloids Metabolism显示文摘在动物农业和药的抗菌素的 overuse 引起了一系列潜在的威胁到公共健康。Macleaya cordata 是从 Papaveraceae 家庭的药用的植物种,提供为抗菌剂的制造的一个安全资源为家畜喂添加剂。从 M. 的活跃成分 ? cordata 被知道包括 benzylisoquinoline 碱(偏爱) 象 sanguinarine (圣) 和 chelerythrine (CHE ) 那样的 ?,而是他们的新陈代谢的小径还得在这非模型植物被学习。在 M. 的圣和 CHE 的活跃生合成 ? cordata 被喂 13标记 C 的酷氨酸。为了增加,推进卓见,我们 de novo 定序 M 的整个染色体。cordata,第一从 Papaveraceae 家庭被定序。M.? 盖住 378 Mb 的 cordata 染色体与 43.5%being 编码 22,328 预言的编码蛋白质的基因 transposable 元素。作为基础 eudicot 的一个成员, M.? cordata 染色体缺乏 ? 发生在几乎所有 eudicots 的 paleohexaploidy 事件。从 genomics 数据, 16 的一个完全的集合 ? 新陈代谢 ? 为圣和 CHE 生合成的基因被检索,并且 14 项他们的生物化学的活动被验证。这些 genomics 和新陈代谢的数据在 M. 显示出保存 BIA 新陈代谢的小径 ? cordata 并且由庄稼改进或微生物引起的小径重建为圣和 CHE 的未来生产提供知识基础。 | Xiubin Liu Yisong Liu Peng Huang Yongshuo Ma Zhixing Qing Qi Tang Huifen Cao Pi Cheng Yajie Zheng Zejun Yuan Yuan Zhou Jinfeng Liu Zhaoshan Tang Yixiu Zhuo Yancong Zhang Linlan Yu Jialu Huang Peng Yang Qiong Peng dinbo Zhang Wenkai Jiang Zhonghua Zhang Kui Lin Dae-Kyun Ro Xiaoya Chen Xingyao Xiong Yi Shang Sanwen Huang Jianguo Zeng | 2017 | Molecular Plant2017，10，7： | 19 |
| 4 | The Chromosome-Based Rubber Tree Genome Provides New Insights into Spurge Genome Evolution and Rubber Biosynthesis显示文摘The rubber tree,Hevea brasiliensis,produces natural rubber that serves as an essential industrial raw material.Here,we present a high-quality reference genome for a rubber tree cultivar GT1 using single-molecule real-time sequencing(SMRT)and Hi-C technologies to anchor the~1.47-Gb genome assembly into 18 pseudochromosomes.The chromosome-based genome analysis enabled us to establish a model of spurge chromosome evolution,since the common paleopolyploid event occurred before the split of Hevea and Manihot.We show recent and rapid bursts of the three Hevea-specific LTR-retrotransposon families during the last 10 million years,leading to the massive expansion by~65.88%(~970 Mbp)of the whole rubber tree genome since the divergence from Manihot.We identify large-scale expansion of genes associated with whole rubber biosynthesis processes,such as basal metabolic processes,ethylene biosynthesis,and the activation of polysaccharide and glycoprotein lectin,which are important properties for latex production.A map of genomic variation between the cultivated and wild rubber trees was obtained,which contains~15.7 million high-quality single-nucleotide polymorphisms.We identified hundreds of candidate domestication genes with drastically lowered genomic diversity in the cultivated but not wild rubber trees despite a relatively short domestication history of rubber tree,some of which are involved in rubber biosynthesis.This genome assembly represents key resources for future rubber tree research and breeding,providing novel targets for improving plant biotic and abiotic tolerance and rubber production. | Jin Liu Cong Shi Cheng-Cheng Shi Wei Li Qun-Jie Zhang Yun Zhang Kui Li Hui-Fang Lu Chao Shi Si-Tao Zhu Zai-Yun Xiao Hong Nan Yao Yue Xun-Ge Zhu Yu Wu Xiao-Ning Hong Guang-Yi Fan Yan Tong Dan Zhang Chang-Li Mao Yun-Long Liu Shi-Jie Hao Wei-Qing Liu Mei-Qi Lv Hai-Bin Zhang Yuan Liu Ge-Ran Hu-tang Jin-Peng Wang Jia-Hao Wang Ying-Huai Sun Shu-Bang Ni Wen-Bin Chen Xing-Cai Zhang Yuan-Nian Jiao Evan E.Eichler Guo-Hua Li Xin Liu Li-Zhi Gao | 2020 | Molecular Plant2020，13，2： | 15 |
| 5 | PRRT2 deficiency induces paroxysmal kinesigenic dyskinesia by regulating synaptic transmission in cerebellum显示文摘在充满脯氨酸的 transmembrane 蛋白质的变化(PRRT2 ) 2 与发作性的 kinesigenic 运动障碍(PKD ) 和几另外的发作性的神经病学的疾病被联系,但是 PRRT2 功能和病原的机制仍然保持大部分阴暗。这里,我们证明 PRRT2 是与 SNARE 建筑群和 downregulates 的部件交往的 presynaptic 蛋白质它的形成。Loss-of-function 变异的老鼠显示出显型由小脑的概括发作,过高热,或 optogenetic 刺激触发了的象 PKD 一样。有在小脑的小粒房间(GC ) 的特定的 PRRT2 删除的变异的老鼠概括在 Prrt2 空的老鼠看见的行为的显型。而且,记录在显示出的小脑的片做了在短暂举起的 GC 结果的刺激由 Purkinje 房间开火的抑制跟随了的那 optogenetic。在 PKD 处理使用的 anticonvulsant 药 carbamazepine 也在变异的老鼠减轻了象 PKD 一样行为。一起,我们的调查结果作为 SNARE 建筑群的一个新奇管理者识别 PRRT2 并且提供位于 PRRT2 相关的行为下面的电路机制。 | Guo-He Tan Yuan-Yuan Liu Lu Wang Kui Li Ze-Qiang Zhang Hong-Fu Li Zhong-Fei Yang Yang Li Dan Li Ming-Yue Wu Chun-Lei Yu Juan-Juan Long Ren-Chao Chen Li-Xi Li Lu-Ping Yin Ji-Wei Liu Xue-Wen Cheng Qi Shen You-Sheng Shu Kenji Sakimura Lu-Jian Liao Zhi-Ying Wu Zhi-Qi Xiong | 2018 | Cell Research2018，28，1： | 14 |
| 6 | IL-37b gene transfer enhances the therapeutic efficacy of mesenchumal stromal cells in DSS- induced colitis mice显示文摘瞄准:调查是否 IL-37b 基因的转移,天生、适应的免疫的一个最新识别的禁止者,能在煽动性的肠疾病( IBD )改进 mesenchumal stromal 房间( MSC )的治疗学的功效 .Methods :在有活跃 ulcerative 大肠炎( UC )的病人的 biopsied 标本的 IL-37 的表示用 RT-PCR 和 immunohistochemistry 被检测。老鼠与 3% 葡聚糖硫酸盐钠(决策支持系统) 被对待 8 天导致大肠炎。在决策支持系统治疗前,老鼠与 MSC, MSC-eGFP 或 MSC-IL37b 被注射。他们的身体重量每天被测量,并且结肠和脾在 d 上被收获 10 为病理学、生物化学的 analyses.Results : 在有活跃 UC 的病人,的 biopsied 标本, IL-37 的表示戏剧性地在煽动的 mucosa 被提高,主要在上皮的房间和渗透的有免疫力的房间。比作 MSC-eGFP 或 MSC, MSC-IL37b 管理显著地稀释了身体重量和冒号长度减小,并且减少在导致决策支持系统的大肠炎老鼠的组织学的分数。而且, MSC-IL37b 管理在老鼠在脾的 CD4+ T 房间之中象规章的 T 房间(Tregs ) 的百分比一样在全部的脾的 mononuclear 房间之中增加了导出 myeloid 的 suppressor 房间(MDSC ) 的百分比。而且, MSC-IL37b 管理增加了 IL-2+ 房间并且减少 IFN-γ在脾的 CD4+ T cells.Conclusion 之中的 + 房间: IL-37 涉及 UC 的 pathophysiology。IL-37b 基因转移由导致 Tregs 和 MDSC 并且调整 cytokine 生产在导致决策支持系统的大肠炎老鼠提高 MSC 的治疗学的功效。 | Wei-qiang WANG Kui DONG Lu ZHOU Guo-hui JIAO Cong-zhong ZHU Wen-wen LI Gang YU Wan-tong WU Song CHEN Zhi-na SUN Yu-ming WANG Wen-tian LIU Jie ZHANG Bang-mao WANG Xiao-ming FENG | 2015 | Acta Pharmacologica Sinica2015，36，11： | 13 |
| 7 | Polydatin prevents the induction of secondary brain injury after traumatic brain injury by protecting neuronal mitochondria显示文摘Polydatin is thought to protect mitochondria in different cell types in various diseases.Mitochondrial dysfunction is a major contributing factor in secondary brain injury resulting from traumatic brain injury.To investigate the protective effect of polydatin after traumatic brain injury,a rat brain injury model of lateral fluid percussion was established to mimic traumatic brain injury insults.Rat models were intraperitoneally injected with polydatin(30 mg/kg)or the SIRT1 activator SRT1720(20 mg/kg,as a positive control to polydatin).At 6 hours post-traumatic brain injury insults,western blot assay was used to detect the expression of SIRT1,endoplasmic reticulum stress related proteins and p38 phosphorylation in cerebral cortex on the injured side.Flow cytometry was used to analyze neuronal mitochondrial superoxide,mitochondrial membrane potential and mitochondrial permeability transition pore opened.Ultrastructural damage in neuronal mitochondria was measured by transmission electron microscopy.Our results showed that after treatment with polydatin,release of reactive oxygen species in neuronal mitochondria was markedly reduced;swelling of mitochondria was alleviated;mitochondrial membrane potential was maintained;mitochondrial permeability transition pore opened.Also endoplasmic reticulum stress related proteins were inhibited,including the activation of p-PERK,spliced XBP-1 and cleaved ATF6.SIRT1 expression and activity were increased;p38 phosphorylation and cleaved caspase-9/3 activation were inhibited.Neurological scores of treated rats were increased and the mortality was reduced compared with the rats only subjected to traumatic brain injury.These results indicated that polydatin protectrd rats from the consequences of traumatic brain injury and exerted a protective effect on neuronal mitochondria.The mechanisms may be linked to increased SIRT1 expression and activity,which inhibits the p38 phosphorylation-mediated mitochondrial apoptotic pathway.This study was approved by the Animal Care and Use Committee of the Southern Medical University,China(approval number:L2016113)on January 1,2016. | Li Li Hong-Ping Tan Cheng-Yong Liu Lin-Tao Yu Da-Nian Wei Zi-Chen Zhang Kui Lu Ke-Sen Zhao Marc Maegele Dao-Zhang Cai Zheng-Tao Gu | 2019 | Neural Regeneration Research2019，14，9： | 12 |
| 8 | The Cambrian sedimentary characteristics and their implications for oil and gas exploration in north margin of Middle-Upper Yangtze Plate显示文摘Using data from tens of measured and observed outcrop successions,thin rock slices and sample analyses,we comprehensively studied the Cambrian sedimentary environments and evolutionary characteristics in the north margin of the Middle-Upper Yangtze Plate.During the Cambrian,platform,slope,and deep sea basin environments were developed in the study area.On the platform,both clastic rocks and carbonate rocks were deposited.Clastic rocks mainly occur in the Lower Cambrian,and were deposited in marine shore and shelf environments.Carbonate rocks are dominant in the Middle and Upper Cambrian,and were deposited in the open platform,restricted platform,tidal flat,beach,and reef environments.Carbonate gravity flow deposits were developed on the slope.In the basin,mainly black shales and chert beds were deposited.The Cambrian represents one large transgression-regression cycle,and maximum transgression occurred in the Qiongzhusi Age of the Early Cambrian.Tectonics and sea level fluctuations had important impacts on sedimentary environments.The Chengkou-Fangxian-Xiangfan Fracture controlled the position of the platform,slope and basin,as well as the silica supply for chert deposition in basin.Sea level fluctuations controlled types of sediments and sedimentary facies on the platform.In the study area,there are good reservoir rocks,including dolomites,grainstones,debris flow deposits,sandstones,and conglomerates;there are good source rocks,including black shales,dark micrites,and chert beds;and there are also good reservoir-source rock assemblages.The hydrocarbon potential of the study area is great. | YU KuanHong JIN ZhenKui SU Kui DONG XiaoDong ZHANG Wei DU HongYu CHEN Ying ZHANG WeiDan | 2013 | Science China Earth Sciences2013，56，6： | 9 |
| 9 | Self-template synthesis of double-layered porous nanotubes with spatially separated photoredox surfaces for efficient photocatalytic hydrogen production显示文摘Improving charge carriers separation to achieve high photoconversion efficiency in heterogeneous photocatalysts is highly desirable.Herein,heterostructured ZnS@CdS double-layered porous nanotubes(PNTs),in which the spatially separated reduction and oxidation reaction sites lie on the outer and inner shell,respectively,are fabricated through a robust self-template conversion strategy.After selective photo-deposition of Ni and CoO_x as dual cocatalysts,Ni nanoparticles as electron collectors and reduction reaction sites are loaded on the outer shell,while CoO_x nanoparticles as hole collectors and oxidation reaction sites are loaded on the inner shells.As a result,a novel CoO_x/ZnS@CdS/Ni photocatalyst is obtained and shows high visible-light-driven photocatalytic hydrogen production activity owing to the synergistic effect of self-template-derived thin mesoporous heterojunctions and photo-depositionderived spatially separated dual cocatalysts,which can significantly provide driving force for the ordered transfer of photogenerated electrons and holes toward opposite direction and promote the surface catalytic reaction.Additionally,the facile strategy can be broadened to the preparation of CoO_x/ZnSe@CdSe/NiPNTs with enhanced photocatalytic activity. | Yani Xin Yi Huang Kui Lin Yifu Yu Bin Zhang | 2018 | Science Bulletin2018，63，10： | 9 |
| 10 | Molecularly imprinted macroporous monolithic materials for protein recognition显示文摘Synthetic materials that can specifically recognize proteins will find wide application in many fields.In this report,bovine serum albumin was chosen as the template protein.Acrylamide and N,N'-methylenebisacrylamide were employed as the functional and cross-linker monomers,respectively.Molecularly imprinted macroporous monolithic materials that can preferentially bind the template protein in an aqueous environment were prepared by combination of molecular imprinting technique and freezing/thawing preparation method.The resulted imprinted macroporous monolithic columns were evaluated by utilizing as stationary phase in high performance liquid chromatography and solid-phase extraction materials.The experimental results indicated that the imprinted macroporous monolithic column exhibited good recognition for template protein,as compared with the control protein(hemoglobin),whereas the non-imprinted polymer(prepared under the same conditions except without addition template protein) had no selective properties. | Qi Liang Deng Yan Li Li Li Hua Zhang Yu Kui Zhang | 2011 | Chinese Chemical Letters2011，22，11： | 8 |
| 11 | The Relationship between Polymorphisms at 17 Gene Sites and Hypertension among the Aboriginal Tibetan People显示文摘Objective The incidence of hypertension in Tibet ranks highest among all Chinese provinces. This may be due to genetic changes caused by Tibet's unique natural environment and agrarian lifestyle, prompting us to investigated the relationship between gene polymorphisms and hypertension. Methods Blood samples were collected from 229 hypertensive participants and 372 healthy (control) participants from five Tibetan counties. Seventeen single nucleotide polymorphisms were investigated for their connection to hypertension. Results The C allele at rs2070744 of the NOS3 gene was shown to be significantly associated with hypertension (P=0.0443; OR=1.636). Additionally, the T allele of rs4961 of the ADD gene was correlated with hypertension in women (P=0.03124; OR=1.584). Conclusion In this study we found that the NOS3 and ADD genes were related to a high incidence of hypertension among Tibetans. NOS3 gene plays a role in regulating vascular tone and blood vessel diameter, which may be altered by the low-oxygen environment of Tibet. ADD is involved in water and salt metabolism, which is consistent with the high-salt diet of Tibetans. The correlations elucidated by our study were different from those of other ethnic groups, indicating that these findings may be specific to the Tibetan people. | LI Kui LIANG Yu SUN Yin ZHANG Ling Xia YI Xin CHEN Yong GU SANG LA MU WANG Jian | 2012 | Biomedical and Environmental Sciences2012，25，5： | 6 |
| 12 | A critical epitope in CD147 facilitates memory CD4^(+) T-cell hyper-activation in rheumatoid arthritis显示文摘The abnormal activation of CD4^(+)CD45RO+memory T(Tm)cells plays an important role in the pathogenesis of rheumatoid arthritis(RA).Previous studies have shown that CD147 participates in T-cell activation.However,it remains unclear whether CD147 is involved in abnormal Tm-cell activation in RA patients.In this study,we demonstrated that CD147 was predominantly upregulated in Tm cells derived from RA patients.The anti-CD147 mAb 5A12 specifically inhibited Tm-cell activation and proliferation and further restrained osteoclastogenesis.Using a structural–functional approach,we depicted the interface between 5A12 and CD147.This allowed us to identify two critical residues,Lys63 and Asp65,as potential targets for RA treatment,as the double mutation K63A/D65A inhibited Tm-cell activation,mimicking the neutralization by 5A12.This study provides not only a theoretical basis for a“CD147-Tm/Osteoclast-RA chain”for the potential prevention and treatment of RA or other T-cell-mediated autoimmune diseases but also a new target for related drug design and development. | Na Guo Sheng Ye Kui Zhang Xiaoling Yu Hongyong Cui Xiangmin Yang Peng lin Minghua Lv Jinlin Miao Yang Zhang Qing Han Rongguang Zhang Zhinan Chen Ping Zhu | 2019 | Cellular & Molecular Immunology2019，16，6： | 6 |
| 13 | Dual-emissive nanohybrid nanoclusters for sensitive ions of carbon dots and gold determination of mercuric显示文摘 | Yehan Yan Huan Yu Kui Zhang Mingtai Sun Yajiao Zhang Xiangke Wang Suhua Wang | 2016 | Nano Research2016，9，7： | 6 |
| 14 | Relationship between Dyslipidemia and Gene Polymorphism in Tibetan Population显示文摘Objective To inves gate the rela onship between SNPs reported in previous studies and the blood lipid level in the Tibetan popula on.Methods Random cluster sampling was employed in 5 areas (Lhasa,Shigatse,Shannan,Nagqu,and Nyingchi).The levels of cholesterol (TC),triglyceride (TG),high-density lipoprotein cholesterol (HDL-C),and low-density lipoprotein cholesterol (LDL-C) from blood samples were determined and DNA was extracted for genotyping and sta s cal analyses.Results Among 1 318 subjects aged >18 years enrolled in this study,367 had dyslipidemia with a prevalence of 27.8%,of whom dyslipidemia males accounted for 33.1% and dyslipidemia females 24.5%.Results of the correla on analysis between all SNPs and TG showed that the SNPs of rs714052 and rs964184 were related to the serum TG level.Subjects with rs714052 CC genotype had the lowest TG level,and the highest TG level was found in those with rs714052 TT genotype.The serum TG level in individuals with TC genotype lied in between the above two popula on groups.Subjects with rs964184 CC genotype had the lowest TG level,and the highest serum TG level was noted in those with rs964184 GG genotype.Conclusion Several SNPs were found to be related to the serum TG level in the Tibetan popula on.The APOA5 gene and MLXIPL gene may be closely associated with the serum TG level in this ethnic popula on group. | ZHANG Ling Xia SUN Ying LIANG Yu LI Kui CHEN Yong GUSANGLAMU WANG Jian | 2012 | Biomedical and Environmental Sciences2012，25，3： | 5 |
| 15 | Synthesis and biological evaluation of ethyl 6-alkoxy-7-phenyl-4-hydroxy-3-quinolinecarboxylates against Eimeria tenella显示文摘A series of ethyl 6-alkoxy-7-phenyl-4-hydroxy-3-quinolinecarboxylates were designed and synthesized.Their structures were confirmed by ~1H NMR,^(13)C NMR,IR and HRMS.The biological activities were primarily evaluated against Eimeria tenella according to Anticoccidial Index(ACI) method in vivo.The results showed that compounds 5e,5f and 5i exhibited anticoccidial activities against E.tenella at 27 mg kg^(-1). | Zhang, Yuan Yuan Zeng, Xing Yan Nie, Kui Zhong, Zhi Cheng Wang, Yu Liang Wang, Yu Zhong | 2010 | Chinese Chemical Letters2010，21，4： | 5 |
| 16 | Unraveling a genetic roadmap for improved taste in the domesticated apple显示文摘Although taste is an important aspect of fruit quality, an understanding of its genetic control remains elusive in apple and other fruit crops. In this study, we conducted genomic sequence analysis of 497 Malus accessions and revealed erosion of genetic diversity caused by apple breeding and possible independent domestication events of dessert and cider apples. Signatures of selection for fruit acidity and size, but not for fruit sugar content, were detected during the processes of both domestication and improvement. Furthermore, we found that single mutations in major genes affecting fruit taste, including Ma1, MdTDT, and MdSOT2, dramatically decrease malate, citrate, and sorbitol accumulation, respectively, and correspond to important domestication events. Interestingly, Ma1 was identified to have pleiotropic effects on both organic acid content and sugar:acid ratio, suggesting that it plays a vital role in determining fruit taste. Fruit taste is unlikely to have been negatively affected by linkage drag associated with selection for larger fruit that resulted from the pyramiding of multiple genes with minor effects on fruit size. Collectively, our study provides new insights into the genetic basis of fruit quality and its evolutionary roadmap during apple domestication, pinpointing several candidate genes for genetic manipulation of fruit taste in apple. | Liao Liao Weihan Zhang Bo Zhang Ting Fang Xiao-Fei Wang Yarning Cai Collins Ogutu Lei Gao Gang Chen Xiaoqing Nie Jinsheng Xu Quanyan Zhang Yiran Ren Jianqiang Yu Chukun Wang Cecilia H.Deng Baiquan Ma Beibei Zheng Chun-Xiang You Da-Gang Hu Richard Espley Kui Lin-Wang Jia-Long Yao Andrew C.Allan Awais Khan Schuyler S.Korban Zhangjun Fei Ray Ming Yu-Jin Hao Li Li Yuepeng Han | 2021 | Molecular Plant2021，14，9： | 5 |
| 17 | Synthesis of texture-excellent mesoporous alumina using PEG1000 as structure-directing agent显示文摘By varying concentration of PEG1000 as a structure-directing agent,mesoporous alumina with excellent textural properties was synthesized.The prepared mesoporous alumina displays high thermal stability,as shown by its textural properties at different calcination temperatures of 600-850 °C.Characterization by SEM and TEM revealed that the added PEG surfactant induced the formation of petal-like alumina.XRD results clarified that all samples were amorphous and their peaks were around the peaks of γ-alumina.N_2 adsorption-desorption analysis showed that the prepared mesoporous alumina,if with PEG1000 in hydrolysis of aluminum isopropoxide,had excellent textural properties with large specific surface area,high pore volume and suitable pore size.The petal-like structure existing in the alumina samples improved their textural parameters,and the role and influential mechanism of PEG1000 were analyzed. | Kui Zhang Changming Li Jian Yu Shiqiu Gao Guangwen Xu | 2017 | Chinese Journal of Chemical Engineering2017，25，1： | 5 |
| 18 | Neuroprotective effects of vagus nerve stimulation on traumatic brain injury显示文摘Previous studies have shown that vagus nerve stimulation can improve the prognosis of traumatic brain injury. The aim of this study was to elucidate the mechanism of the neuroprotective effects of vagus nerve stimulation in rabbits with brain explosive injury. Rabbits with brain explosive injury received continuous stimulation(10 V, 5 Hz, 5 ms, 20 minutes) of the right cervical vagus nerve. Tumor necrosis factor-α, interleukin-1β and interleukin-10 concentrations were detected in serum and brain tissues, and water content in brain tissues was measured. Results showed that vagus nerve stimulation could reduce the degree of brain edema, decrease tumor necrosis factor-α and interleukin-1β concentrations, and increase interleukin-10 concentration after brain explosive injury in rabbits. These data suggest that vagus nerve stimulation may exert neuroprotective effects against explosive injury via regulating the expression of tumor necrosis factor-α, interleukin-1β and interleukin-10 in the serum and brain tissue. | Long Zhou Jinhuang Lin Junming Lin Guoju Kui Jianhua Zhang Yigang Yu | 2014 | Neural Regeneration Research2014，9，17： | 4 |
| 19 | Molecular Epidemiology of Coxsackievirus B1-5 Associated with HFMD in Fujian Province, China, 2011-2016显示文摘Hand,foot and mouth disease(HFMD)is a common infectious disease that usually affects children less than 5 years of age.HFMD is caused by human enteroviruses(HEVs).HEVs,members of the Enterovirus genus of the Picornaviridae(small RNA virus)family. | CHEN Wei WENG Yu Wei ZHANG Yong Jun HE Wen Xiang ZHU Ying YU Ting Ting XIE Jian Feng ZHENG Kui Cheng YAN Yan Sheng ZHANG Wen Chang | 2019 | Biomedical and Environmental Sciences2019，32，8： | 4 |
| 20 | RT-nPCR Assays for Amplification and Sequencing of VP1 Genes in Human Enterovirus A–D from Clinical Specimens显示文摘Objective To develop RT-nPCR assays for amplifying partial and complete VP1 genes of human enteroviruses(HEVs)from clinical samples and to contribute to etiological surveillance of HEV-related diseases.Methods A panel of RT-nPCR assays,consisting of published combined primer pairs for VP1 genes of HEV A–C and in-house designed primers for HEV-D,was established in this study.The sensitivity of each RT-nPCR assay was evaluated with serially diluted virus stocks of five serotypes expressed as CCID50 perμL and copies perμL,and the newly established methods were tested in clinical specimens collected in recent years.Results The sensitivity of RT-nPCR assays for amplifying partial VP1 gene of HEVs was 0.1 CCID50 perμL and 10 virus copies perμL,and for the complete VP1 gene was 1 CCID50 perμL and 100 virus copies perμL,using serially-diluted virus stocks of five serotypes.As a proof-of-concept,25 serotypes were identified and complete VP1 sequences of 23 serotypes were obtained by this system among 858 clinical specimens positive for HEVs during the past eight surveillance seasons.Conclusion This RT-nPCR system is capable of amplifying the partial and complete VP1 gene of HEV A–D,providing rapid,sensitive,and reliable options for molecular typing and molecular epidemiology of HEVs in clinical specimens. | CHEN Wei WENG Yu Wei HE Wen Xiang ZHU Ying YU Ting Ting XIE Jian Feng ZHENG Kui Cheng YAN Yan Sheng ZHANG Yong Jun ZHANG Wen Chang | 2020 | Biomedical and Environmental Sciences2020，33，11： | 4 |
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