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| 1 | Microvessel changes in the gerbil hippocampus after cerebral ischemia and reperfusion by Buyang Huanwu decoction pretreatment显示文摘Buyang Huanwu decoction(BYHWD) is a classic recipe for the prevention and treatment of ischemic cerebrovascular disease.Gerbils were pretreated with BYHWD,and then subjected to cerebral ischemia and reperfusion.Microvascular changes were determined with laser Doppler monitoring,tannic acid-ferric chloride mordant,and electron microscopy.Results showed that BYHWD pretreatment could enhance the function of hippocampal microvessels,prevent injury,and increase microvasular density and microvasular area density.Thus,these results suggest that BYHWD pretreatment could prevent microvascular occlusion,enhance the capacity of microvascular reperfusion,increase cerebral blood flow,and inhibit neuronal damage,and may be an effective therapy against brain ischemic injury. | Xiaoguang Wu Yixue Li Haixia Liu Yuhua Yin Shumin Zhao Yuanyuan Guo | 2011 | Neural Regeneration Research2011，6，9： | 11 |
| 2 | The molecular determinants governing the immunogenicity of Japanese encephalitis live attenuated vaccines显示文摘In the course of isolating the attenuated Japanese encephalitis vaccine SA14-14-2,two attenuated strains SA14-9-7 and SA14-5-3 were also obtained that elicited low antibody responses in humans(o10%and 62%,respectively)and exerted much weaker immune protection in animal challenge experiments.However,the reason for these differences remains unknown.In order to understand why SA14-14-2 is superior to SA14-9-7 and SA14-5-3,we employed a reverse genetics method to identify the key mutations in the virus genome that determine the immunogenicity of live attenuated Japanese encephalitis viruses.We first sequenced the full genomic sequences of SA14-9-7 and SA14-5-3 and found mutations that changed four amino-acid base pairs when compared to the envelope gene of SA14-14-2.We mutated the genome of SA14-14-2 to generate these mutations both singly(E-177,E-264,E-279 and E-315)and in combination(E-177/264,E-279/315 and E-177/264/279/315)and tested these mutants along with parental strains SA14-14-2,SA14-9-7 and SA14-5-3 for their immunogenicity in vivo.When mice were immunized with SA14-9-7 and SA14-5-3,lower levels of neutralizing antibodies were generated compared with the immune response to SA14-14-2.Furthermore,SA14-5-3 was more immunogenic than SA14-9-7,which replicated the results previously seen in humans.Point mutations E-177,E-264,E-279 and E-315 diminished the immunogenicity of SA14-14-2 with E-264 and E-315,contributing the most to this phenotype.The mutant rJEV(E-177/E-264/E-279/E-315)containing all four point mutations exhibited the lowest immunogenicity with a seroconversion rate of 0 at an inoculation dose of 103 PFU(plaque-forming unit).We have identified the key amino acids in the envelope protein that account for the superior immunogenicity of SA14-14-2. | Yuhua Li Yin Fu Xinyu Liu Huiqiang Yang Yongxin Yu Lili Jia Xuguang Li Aaron Farnsworth Junzhi Wang | 2017 | Signal Transduction and Targeted Therapy2017，2，1： | 3 |
| 3 | Two-stage degradation and novel functional endothelium characteristics of a 3-D printed bioresorbable scaffold显示文摘Bioresorbable scaffolds have emerged as a new generation of vascular implants for the treatment of atherosclerosis,and designed to provide a temporary scaffold that is subsequently absorbed by blood vessels over time.Presently,there is insufficient data on the biological and mechanical responses of blood vessels accompanied by bioresorbable scaffolds(BRS)degradation.Therefore,it is necessary to investigate the inflexion point of degradation,the response of blood vessels,and the pathophysiological process of vascular,as results of such studies will be of great value for the design of next generation of BRS.In this study,abdominal aortas of SD rats were received 3-D printed poly-l-actide vascular scaffolds(PLS)for various durations up to 12 months.The response of PLS implanted aorta went through two distinct processes:(1)the neointima with desirable barrier function was obtained in 1 month,accompanied with slow degradation,inflammation,and intimal hyperplasia;(2)significant degradation occurred from 6 months,accompanied with decreasing inflammation and intimal hyperplasia,while the extracellular matrix recovered to normal vessels which indicate the positive remodeling.These in vivo results indicate that 6 months is a key turning point.This“two-stage degradation and vascular characteristics”is proposed to elucidate the long-term effects of PLS on vascular repair and demonstrated the potential of PLS in promoting endothelium function and positive remodeling,which highlights the benefits of PLS and shed some light in the future researches,such as drug combination coatings design. | Tieying Yin Ruolin Du Yang Wang Junyang Huang Shuang Ge Yuhua Huang Youhua Tan Qing Liu Zhong Chen Hanqing Feng Jie Du Yazhou Wang Guixue Wang | 2022 | Bioactive Materials2022，7，4： | 2 |
| 4 | Comprehensive Multi-Level Exploration of Buried Active Faults:an Example of the Yinchuan Buried Active Fault显示文摘The paper introduces the steps and methods of multi-approach,multi-level exploration of buried faults in thick Quaternary sediment regions by taking the test exploration of the Yinchuan active fault as example.Based on the comprehensive analyses of previous data,we choose the Xinqushao Village of Xingqing District of Yinchuan City as the test site for the comprehensive exploration.Firstly,we adopted shallow seismic investigation with group intervals of 10m,5m and 1m to gradually trace layer by layer the master fault of the Yinchuan buried fault from a deep depth to a shallow depth where drilling could be used.Then,with composite geological profile drilling,we determined the precise location and dip angle of the fault.The drilling show the buried depth of the upper offset point is 8.3m.Finally,large-scale trenching revealed that the actual buried depth of the upper offset point of the fault is 1.5m from the ground surface and there are paleoearthquake events of 5 stages.Combined with the preliminary result of corresponding sample age,we conclude the Yinchuan buried fault is a mid to late Holocene active fault. | Chai Chizhang Meng Guangkui Du Peng Wang Yin Liu Baojin Shen Weihua LeiQiyun Liao Yuhua Zhao Chengbin Feng Shaoying Zhang Xuehui Xie Xiaofeng | 2007 | Earthquake Research in China2007，21，3： | 1 |
| 5 | Simulated annealing study of diblock copolymer thin films confined between two homogeneous surfaces 显示文摘 | Yin Yuhua Sun Pingchuan Chen Tiehong | 2004 | ChemPhysChem2004，，5： | 1 |
| 6 | Simulated annealing study of morphological transitions of diblock copolymers in solution显示文摘 | Sun Pingchuan Yin Yuhua Li Baohui | 2005 | J Chem Phys2005，122，： | 1 |
| 7 | Adaptive fractional-order switching-type control method design for 3D frac- tional-order nonlinear systems显示文摘 | YIN Chun CHENG Yuhua CHEN Yangquan | 2015 | Nonlinear Dyn2015，85，12： | 1 |
| 8 | Rapid determination of water- and fat-soluble vitamins with microemulsion electrokinetic chromatography显示文摘 | YIN Changna CAO Yuhua DING Shaodong | 2008 | J Chromatogr: A2008，1193，12： | 1 |
| 9 | Energy-based adaptive orthogonal FRIT and its application in image denoising显示文摘Efficient representation of linear singularities is discussed in this paper. We analyzed the relationship between the “wrap around” effect and the distribution of FRAT (Finite Radon Transform) coefficients first, and then based on study of some properties of the columnwisely FRAT reconstruction procedure, we proposed an energy-based adaptive orthogonal FRIT scheme (EFRIT). Experiments using nonlinear approximation show its superiority in energy concentration over both Discrete Wavelet Transform (DWT) and Finite Ridgelet Transform (FRIT). Furthermore, we have modeled the denoising problem and proposed a novel threshold selecting method. Experiments carried out on images containing strong linear singularities and texture components with varying levels of addictive white Gaussian noise show that our method achieves prominent improvement in terms of both SNR and visual quality as compared with that of DWT and FRIT. | LIU YunXia PENG YuHua QU HuaiJing YIN Yong | 2007 | Science in China(Series F)2007，50，2： | 1 |
| 10 | TiO2/TiO2-xNynanocomposite and its acetaldehyde photodecomposition ability显示文摘 | Liu Bin Wang Yuhua Yin Shu | | 0，，： | 1 |
| 11 | Immunomodulation of Rheum tanguticum polysaccharide (RTP) on the immunosuppressive effects of dexamethasone (DEX) on the treatment of colitis in rats induced by 2,4,6-trinitrobenzene sulfonic acid显示文摘 | Li Liu Shifang Yuan Yin Long Zhenjun Guo Yang Sun Yuhua Li Yinbo Niu Chen Li Qibing Mei | 2009 | International Immunopharmacology2009，，13： | 1 |
| 12 | Microstrueture and Properties of (SIC, TiB2)/B4C Composites by Reaction Hot-pressing显示文摘 | Li Aiju Zhen Yuhua Yin Qiang | 2006 | Ceramics International2006，32，8： | 1 |
| 13 | The synthesis of PROTAC molecule and new target KAT6A identification of CDK9 inhibitor i CDK9显示文摘Cyclin-dependent kinases(CDKs) have become potential targets for treating various diseases, especially cancer. Compound i CDK9 is an excellent and selective CDK9 inhibitor, but its major limitation is the potential toxicity and poor understanding of the underlying mechanism. The PROTAC(proteolysis targeting chimera) degraders of bioactive molecules can significantly induce in vitro and in vivo degradation of their target protein with high selectivity and effectively reduce the dose-limiting toxicity of small molecule drugs. Therefore, we designed and synthesized the bifunctional PROTAC molecules of i CDK9, being used for identifying its previously unknown target and revealing the underlying pharmacological mechanism.The PROTAC bifunctional molecule CD-5 could selectively and significantly degrade CDK9 with low cell toxicity. Therefore, we selected CD-5 as a chemical prober in the SILAC quantitative proteomic analysis, which disclosed that CD-5 could enormously lessen the lysine acetyltransferase KAT6A. Furthermore,KAT6A degradation induced by CD-5 repressed the levels of H3K14Ac and H3K23Ac. Lastly, the streptavidin immunoprecipitation(IP) assay confirmed a direct interaction between KAT6A and i CDK9. Collectively, our results uncover that KAT6A is a potential non-kinase target of i CDK9. Notably, this study also demonstrates that the PROTAC-SILAC strategy is an alternative approach for cellular target identification of bioactive molecules. | Mingtao Ao Jun Wu Yin Cao Yaohui He Yuxiang Zhang Xiang Gao Yuhua Xue Meijuan Fang Zhen Wu | 2023 | Chinese Chemical Letters2023，34，4： | 1 |
| 14 | Reactive and Dense Sintering of Reinforced-toughened B4C Matrix Composites显示文摘 | Zhen Yuhua Li Aiju Yin Yansheng | 2004 | Materials Research Bulletin2004，39，： | 1 |
| 15 | Incidence of I-IIV, hepatitis C and hepatitis B viruses among injection drug users in southwest- em China: a 3-year follow-up study显示文摘 | Ruan Yuhua Qin G Yin Lu | 2007 | AIDS2007，218，8： | 1 |
| 16 | Newly emergent magnetic flux and activation of filaments显示文摘 | Yin ChunLin Tang YuHua | 1995 | ChA&A1995，19，3： | 1 |
| 17 | Microstruct ure and properties of (SIC, TiB2 )/B4 C composites by reaction hot pressing显示文摘 | Li Aiju Zhen Yuhua Yin Qiang Ma Laipeng Yin Yansheng | 2006 | Ceramics International2006，32，8： | 1 |
| 18 | Preparation and Properties of Waterborne Polyurethane Adhesives Modified by Polystyrene显示文摘 | Hui Du Yuhua Zhao Ning Yin | 2008 | JAppl Polym Sci2008，110，： | 1 |
| 19 | Improving agronomic practices to reduce nitrate leaching from the rice–wheat rotation system显示文摘 | Yansheng Cao Yuhua Tian Bin Yin Zhaoliang Zhu | 2014 | Agriculture, Ecosystems and Environment2014，，： | 1 |
| 20 | Whole genome variants across 57 pig breeds enable comprehensive identification of genetic signatures that underlie breed features显示文摘Background:A large number of pig breeds are distributed around the world,their features and characteristics vary among breeds,and they are valuable resources.Understanding the underlying genetic mechanisms that explain across-breed variation can help breeders develop improved pig breeds.Results:In this study,we performed GWAS using a standard mixed linear model with three types of genome variants(SNP,InDel,and CNV)that were identified from public,whole-genome,sequencing data sets.We used 469 pigs of 57 breeds,and we identified and analyzed approximately 19 million SNPs,1.8 million InDels,and 18,016 CNVs.We defined six biological phenotypes by the characteristics of breed features to identify the associated genome variants and candidate genes,which included coat color,ear shape,gradient zone,body weight,body length,and body height.A total of 37 candidate genes was identified,which included 27 that were reported previously(e.g.,PLAG1 for body weight),but the other 10 were newly detected candidate genes(e.g.,ADAMTS9 for coat color).Conclusion:Our study indicated that using GWAS across a modest number of breeds with high density genome variants provided efficient mapping of complex traits. | Jingya Xu Yuhua Fu Yan Hu Lilin Yin Zhenshuang Tang Dong Yin Mengjin Zhu Mei Yu Xinyun Li Yang Zhou Shuhong Zhao Xiaolei Liu | 2021 | Journal of Animal Science and Biotechnology2021，12，2： | 1 |
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