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| 1 | Homozygous loss-of-function mutations in FSIP2 cause male infertility with asthenoteratospermia显示文摘Male infertility, as a major issue of human reproduction health, prevents successful natural conception. Asthenoteratospermia mainly presents one or multiple anomalies in head, neck and tail of spermatozoa, and impairs sperm function and motility (Coutton et al., 2015). Recurrent abnormalities of the fibrous sheath lead to multiple morphological abnormaliries of the sperm flagella (MMAF), which is a quite frequent type of asthenoteratospermia in male infertility (Chemes et al., 1987;Ben Khelifa et al, 2014). | Wangjie Liu Huan Wu Li Wang Xiaoyu Yang Chunyu Liu Xiaojin He Weiyu Li Jiajia Wang Yujie Chen Hongyan Wang Yang Gao Shuyan Tang Shenmin Yang Li Jin Feng Zhang Yunxi Cao | 2019 | Journal of Genetics and Genomics2019，46，1： | 7 |
| 2 | Neutrophil-derived heparin binding protein triggers vascular leakage and synergizes with myeloperoxidase at the early stage of severe burns(With video)显示文摘Background:Burn shock caused by vascular leakage is one of the main causes of high mortality in severe burn injury.However,the pathophysiological mechanism of vascular leakage is still unclear.The purpose of this study was to explore the molecular mechanism of vascular leakage in the early stage of severe burn and provide a new target for the treatment of severe burns.Methods:Neutrophils were isolated from human peripheral blood by magnetic beads sorting.ELISA was used to detect neutrophil-derived granule proteins and glycocalyx injury products in plasma.The vascular leakage and neutrophil movement were assessed by in vivo laser confocal imaging in mice,and high-quality video were provided.Adhesion-related molecules were investigated by qRT-PCR.The damage to glycocalyx of mice vascular endothelial cellswas observed by transmission electron microscope and scanning electron microscope.Proteomic analysis,flow cytometry and immunofluorescence were used to further study the relationship between human peripheral blood neutrophil-derived hypochlorite(HOCl)and CD44 of human vascular endothelial cells.Results:In this study,we found that rapidly increasing activated neutrophils secrete heparin binding protein(HBP)andmyeloperoxidase(MPO)after severe burn injury.Increased HBP triggers vascular leakage with synergy of MPO,results in systemic edema and burn shock.Furthermore,we found that the MPO catalytic product HOCl but not MPO triggers CD44 extracellular domain shedding from vascular endothelial cells to damage the glycocalyx.Damage to the glycocalyx results in firm adhesion of neutrophils and increases vascular leakage.However,MPO inhibitors partially protect the glycocalyx of vascular endothelial cells.The combination of HBP and MPO inhibitors markedly reduces vascular leakage and systemic edema in the early stage of severe burns.Conclusions:Taken together,these data reveal that neutrophil-derived HBP and MPO play an important synergies role in triggering vascular leakage at the early stage of severe burns.Targeted intervention in these two biomolecules may introduce new strategies for helping to reduce large amount of fluid loss and subsequent burn shock. | Lu Liu Yiming Shao Yixuan Zhang Yunxi Yang Jiamin Huang Linbin Li Ran Sun Yuying Zhou Yicheng Su Bingwei Sun | 2021 | Burns & Trauma2021，9，1： | 3 |
| 3 | Neutrophil extracellular traps contribute to myofibroblast differentiation and scar hyperplasia through the Toll-like receptor 9/nuclear factor Kappa-B/interleukin-6 pathway显示文摘Background:Inflammation is an important factor in pathological scarring.The role of neutrophils,one of the most important inflammatory cells,in scar hyperplasia remains unclear.The purpose of this article is to study the correlation between neutrophil extracellular traps(NETs)and scar hyperplasia and identify a new target for inhibiting scar hyperplasia.Methods:Neutrophils were isolated from human peripheral blood by magnetic-bead sorting.NETs in plasma and scars were detected by enzyme-linked immunosorbent assays(ELISAs),immunofluorescence and flow cytometry.Immunohistochemistry was used to assess neutrophil(CD66B)infiltration in hypertrophic scars.To observe the entry of NETs into fibroblasts we used immunofluorescence and flow cytometry.Results:We found that peripheral blood neutrophils in patients with hypertrophic scars were more likely to form NETs(p<0.05).Hypertrophic scars showed greater infiltration with neutrophils and NETs(p<0.05).NETs activate fibroblasts in vitro to promote their differentiation and migration.Inhibition of NETs with cytochalasin in wounds reduced the hyperplasia of scars in mice.We induced neutrophils to generate NETs with different stimuli in vitro and detected the proteins carried by NETs.We did not find an increase in the expression of common scarring factors[interleukin(IL)-17 and transforming growth factor-β(TGF-β),p>0.05].However,inhibiting the production of NETs or degrading DNA reduced the differentiation of fibroblasts intomyofibroblasts.In vitro,NETs were found to be mediated by Toll-like receptor 9(TLR-9)in fibroblasts and further phosphorylated nuclear factor Kappa-B(NF-κB).We found that IL-6,which is downstream of NF-κB,was increased in fibroblasts.Additionally,IL-6 uses autocrine and paracrine signaling to promote differentiation and secretion.Conclusions:Our experiments found that NETs activate fibroblasts through the TLR-9/NF-κB/IL-6 pathway,thereby providing a new target for regulating hypertrophic scars. | Yiming Shao Zaiwen Guo Yunxi Yang Lu Liu Jiamin Huang Yi Chen Linbin Li Bingwei Sun | 2022 | Burns & Trauma2022，10，1： | 2 |
| 4 | Delay-range-dependent exponential stability criteria and decay estimation for switchedHopfield neural networks of neural type显示文摘 | ZONG Guangdeng LIU Jia ZHANG Yunxi | 2010 | Nonlinear Analysis2010，4，3： | 1 |
| 5 | Th1 and Th2 cytokine profiles induced by hepatitis C virus F protein in peripheral blood mononuclear cells from chronic hepatitis C patients显示文摘 | Ming Yue Xiaozhao Deng Xiangjun Zhai Ke Xu Jing Kong Jinhai Zhang Zhenxian Zhou Xiaojie Yu Xiaodong Xu Yunxi Liu Danyan Zhu Yun Zhang | 2013 | Immunology Letters2013，，2： | 1 |
| 6 | Potential prebiotic effects of nonabsorptive components of Keemun and Dianhong black tea:an in vitro study显示文摘Black tea is a healthy and popular tea beverage.However,its main bioactive compounds(theaflavins and thearubigins)are not easily absorbed.The aim of this study was to investigate the modulation of intestinal microbiota by the nonabsorptive components of Keemun black tea(KBT)and Dianhong black tea(DBT),and fructooligosaccharide(FOS)was selected for use in the control group.KBT,DBT,and FOS significantly increased total short-chain acid production.Specifically,FOS treatment predominantly increased the production of acetic acids and black tea treatments increased the production of acetic,propionic,and butyric acids at similar rates.Moreover,FOS exerted a strong bifidogenic effect after 24 h of fermentation;KBT and DBT increased the abundance of the beneficial genus Bacteroides and Roseburia.In summary,the nonabsorptive components of KBT and DBT could serve as novel prebiotics,the underlying mechanisms of which are quite different from those of FOS. | Wenjing Liao Wenjiao Li Suyu Liu Dong Tang Yunxi Chen Yijun Wang Zhongwen Xie Jinbao Huang | 2022 | Food Science and Human Wellness2022，11，3： | 1 |
| 7 | Quantitative determination of diterpenoid alkaloid Fuziline by hydrophilic interaction liquid chromatography (HILIC)–electrospray ionization mass spectrometry and its application to pharmacokinetic study in rats显示文摘 | Jianguo Sun Fengyi Zhang Ying Peng Jinghan Liu Yunxi Zhong Guangji Wang | 2013 | Journal of Chromatography B2013，，： | 1 |
| 8 | Clinical applica- tion of RT-nested PCR integrated with RFLP in Hantavirus detection and genotyping: a prospective study in Shandong Province, PR China 显示文摘 | Liu Yunxi Zhao Zhongtang Cao Wuchun | 2013 | Cell Biochem Biophys2013，67，3： | 1 |
| 9 | Epidemiological study of autumn-winter type scrub typhus in a new endemic focus of Fei county,Shandong province,China显示文摘 | Liu yunxi Yang zhanqing Wu qinyong | 2000 | Systematic & Applied Acarology2000，5，： | 1 |
| 10 | Hospital biosecurity capacitation:Analysis and recommendations for the prevention and control of COVID-19显示文摘The outbreak of the coronavirus disease 2019(COVID-19)in December 2019 highlighted several concerns regarding hospital biosafety capacitation in the People’s Republic of China,although the epidemic is now under control.This study examined the primary problems related to hospital biosecurity,including the absence of a hospital emergency system,inadequate management and control of nosocomial infection,limited hospital laboratory capacity,and poor hospital admission capacity.Accordingly,this study puts forward the following countermeasures and suggestions for hospitals to deal with future biosecurity events,such as a major epidemic:first,biosecurity management systems and emergency response mechanisms in hospitals need to be set up;second,the investment and guarantee mechanisms for hospital biosecurity construction should be improved;third,the capacity building of biosecurity incident management requires special attention in general hospitals;and finally,comprehensive plans need to be developed for the integrated construction of medical treatment and prevention facilities through diseasecontrol systems. | Hui Ma Jiangong Zhu Jianfei Liu Xin Zhang Yunxi Liu Quansheng Yang | 2020 | Journal of Biosafety and Biosecurity2020，2，1： | 1 |
| 11 | Serological reports of human infections of H7 and H9 avian influenza viruses in northern China 显示文摘 | Jia Na de Vlas SJ Liu Yunxi | 2009 | J Clin Virol2009，44，3： | 1 |
| 12 | The decoupling relationship between China’s economic growth and carbon emissions from the perspective of industrial structure显示文摘This study analyses the decoupling relationship between energy-related CO_(2) emissions and economic growth in China from 2000-2017 from the perspective of industrial structure using the LMDI method and Tapio decoupling model.The decoupling driving force was decomposed into emission,energy intensity,industrial structure,economic activity,and population factors.Studies have shown that the restructuring of China’s industries has not led to a significant increase in CO_(2) emissions.Specifically,from 2000 to 2017,China’s economic development and carbon emissions are generally in a weak decoupling state.Strong decoupling occurred from 2000 to 2001 and from 2013 to 2016.The industrial structure change has a substantial impact on the primary sector(agriculture),followed by the tertiary sector(services),while the impact on the secondary sector(industry)is negligible.The GDP contribution of the primary industry is small,but it is an indispensable guarantee for a country.With the development of agricultural mechanization and technology,human and animal power in this industry has been decreasing year by year,and large-scale planting has improved energy efficiency.The level of technology should be continuously strengthened to promote the decoupling of carbon emissions.The contribution of the secondary industry to GDP is around 40%,but the secondary industry accounts for more than 80%of CO_(2) emissions,mainly caused by the use of fossil energy.In 2017,fossil energy still accounted for about 86.2%of China’s energy consumption,so fossil energy remains the most critical energy source for China’s economic development.Coal,in turn,accounts for the largest share of fossil energy consumption.Therefore,emphasis should be placed on increasing technological innovation and conversion from fossil to non-fossil energy sources.The Chinese government considers the service industry a new engine for sustainable social and economic development.The contribution of the tertiary sector to GDP has increased from 2000 to 2017,followed by resource and environmental problems.Although the current share of CO_(2) emissions directly caused by the tertiary sector is not high,as the tertiary sector continues to grow,plans to reduce the decoupling of carbon emissions from the tertiary sector should be made in advance. | Yuwei Du Yunxi Liu Md.Afzal Hossain Songsheng Chen | 2022 | Chinese Journal of Population,Resources and Environment2022，20，1： | 1 |
| 13 | Epidemiology, resistant pathogens, and causes of early death in cases of bloodstream infection in patients with hematological malignancies from 2012-2019显示文摘Purpose:To investigate the epidemiology,causative pathogen antibiotic susceptibility,and mortality risk factors of bloodstream infection(BSI)in patients with hematological malignancies(HMs).Methods:Single-center retrospective analysis of BSI cases in patients with HMs in a Chinese tertiary hospital from 2012-2019.Results:Among 17,796 analyzed admissions,508 BSI episodes(2.9%;95%confidence interval:2.6%-3.2%)were identified.Of 522 resulting isolates,326(62.5%)were Gram-negative,173(33.1%)were Gram-positive.The BSI incidence among patients with different HMs(severe aplastic anemia:6.7%;acute leukemia:6.2%;myelodysplastic syndrome:3.2%;multiple myeloma:1.3%;and lymphoma:1.0%)differed significantly(p<0.001).The BSI incidence was significantly higher in the hematopoietic stem cell transplantation(HSCT)group(10.2%)than in the non-HSCT group(2.5%;p<0.001).Escherichia coli(30.7%,160/522)was the most common pathogen,followed by Coagulase-negative staphylococci(19.4%,101/522)and Klebsiella pneumoniae(10.0%,52/522).,The rates of imipenem resistance for E.coli,K.pneumoniae,Pseudomonas aeruginosa,and Acinetobacter baumannii were 6.4%,15.0%,27.8%,and 79.0%,respectively.All the Gram-positive pathogens were linezolid susceptible.Three vancomycin-resistant Enterococcus species were isolated.The overall 14-day mortality was 9.8%(95%confidence interval:7.2%-12.4%).A multivariate analysis showed that HM subtype severe aplastic anemia,A.baumannii,and malignancy non-remission were independent 14-day mortality risk factors.Conclusions:Gram-negative bacteria were the most common pathogens,with E.coli as the predominant strain,causing BSIs in HM patients.A carbapenem-resistant A.baumanni with a high mortality rate in HM patients made empirical antimicrobial choice a highly challenging issue. | Meng Li Mingmei Du Honghua Li Daihong Liu Yunxi Liu | 2022 | Infectious Medicine2022，1，1： | 0 |
| 14 | Temporal and Spatial Distribution of SARS-CoV-2 Aerosols in a Large-Scale Fangcang Shelter Hospital in Shanghai,China显示文摘The coronavirus disease 2019(COVID-19)pandemic caused by frequently mutating severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has had a worldwide impact.However,detailed data on the potential aerosol transmission of SARS-CoV-2 in real-world and controlled laboratory settings remain sparse.During the COVID-19 pandemic in Shanghai,China in 2022,samples were collected in a Fangcang shelter hospital,a large-scale temporary hospital rapidly built by converting the existing National Exhibition and Convention Center(Shanghai)into a health care facility.Aerosol samples at different sites and intervals around patients and in public areas,surface samples,and pharyngeal swab samples from corresponding patients were included.Samples were tested for SARS-CoV-2 using real-time quantitative polymerase chain reaction(RT-qPCR)assays,followed by sequencing if the cycle threshold(Ct)value was<30.The positivity rate for SARS-CoV-2 in aerosol samples was high in contaminated zones(37.5%,104/277),especially around the bed(41.2%,68/165)and near ventilation inlets(45.2%,14/31).The prevalence of SARS-CoV-2 around the bed,public areas,and air inlets of exhaust vents fluctuated and was closely related to the positivity rate among patients at corresponding sampling sites.Some surface samples of different personal protective equipment from medical staff had high positivity rates.Sixty sequences of joined ORF1ab and spike genes obtained from sixty samples represented two main clusters of Omicron SARS-CoV-2.There was consistency in virus sequences from the same patient and their environment,and the detected virus sequences matched those of virus strains in circulation during the collection periods,which indicated a high likelihood of cross-contamination in the Fangcang shelter hospital.In summary,the results provide a quantitative and real landscape of the aerosol transmission of SARS-CoV-2 and a patient-centered view of contamination in large and enclosed spaces and offer a useful guide for taking targeted measures to avoid nosocomial infections during the management of SARS-CoV-2 or other respiratory virus diseases in a Fangcang shelter hospital. | Jiafu Jiang Zhe Yin Jing Li Leili Jia Rulin He Wenhui Yang Jihu Yang Hang Fan Sen Zhang Yunfei Wang Zengming Zhao Haoran Peng Lizhong Li Yi Yang Shi-Yong Fan Rong Xiang Jianshu Guo Jinjin Wang Juanning Wei Fengling Zhou Ding Liu Ping Zhao Yujun Cui Yunxi Liu Dongsheng Zhou Gang Dong | 2023 | Engineering2023，，9： | 0 |
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