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| 1 | The photoluminescence mechanism in carbon dots (graphene quantum dots, carbon nanodots, and polymer dots): Current state and future perspective显示文摘目前,光致发光(PL ) 的实际机制荧光灯碳点(CD ) 仍然是在研究人员之中的开的争论。因为 CD 的变化,为这些种碳材料总结 PL 机制是高度重要的;那么做能指导有效合成线路和新奇应用程序的开发。这评论将集中于 CD 的 PL 机制。三种类型荧光灯 CD 被包含:graphene 量点(GQD ) ,碳 nanodots (CND ) ,和聚合物点(PD ) 。四合理 PL 机制被证实了:被碳核心决定的量监禁效果或结合的 -domains, ;表面状态,它由碳脊梁和连接的化学的组的杂交坚定;分子状态,它完全坚定由荧光灯分子在 CD 的表面或内部上连接了;并且提高交叉连接的排放(C ) 效果。为 CD 给一篇彻底的摘要,范畴和合成线路,以及化学 / 物理的性质,简短预先被介绍。 | Shoujun Zhu Yubin Song Xiaohuan Zhao Jieren Shao Junhu Zhang Bai Yang | 2015 | Nano Research2015，8，2： | 120 |
| 2 | Analysis of stable components for extended-range (10-30 days) weather forecast:A case study of continuous overcast-rainy process in early 2009 over the mid-lower reaches of the Yangtze River显示文摘A continuous overcast-rainy weather(CORW) process occurred over the mid-lower reaches of the Yangtze River(MLRYR) in China from February 14 to March 9 in 2009,with a large stretch and long duration that was rarely seen in historical records.Using the empirical orthogonal function(EOF),we analyzed the geopotential height anomaly field of the NCEP-DOE Reanalysis II in the same period,and defined the stable components of extended-range(10-30 days) weather forecast(ERWF).Furthermore,we defined anomalous and climatic stable components based on the variation characteristics of the variance contribution ratio of EOF components.The climatic stable components were able to explain the impact of climatically averaged information on the ERWF,and the anomalous stable components revealed the abnormal characteristics of the continuous overcast-rainy days.Our results show that the stable components,especially the anomalous stable components,can maintain the stability for a longer time(more than 10 days) and manifest as monthly scale low-frequency variation and ultra-long-wave activities.They also behave as ultra-long waves of planetary scale with a stable and vertically coherent structure,reflect the variation of general circulation in mid-high latitudes,display the cycle of the zonal circulation and the movement and adjustment of the ultra-long waves,and are closely linked to the surface CORW process. | FENG GuoLin SUN ShuPeng ZHAO JunHu ZHENG ZhiHai | 2013 | Science China Earth Sciences2013，56，9： | 8 |
| 3 | Dynamic-statistics combined forecast scheme based on the abrupt decadal change component of summer precipitation in East Asia显示文摘基于 19832011 CMAP 数据,在东亚的降水异例和它的附近的海区域(此后叫的东亚为短) 在 1999 前表明 ++ 模式并且 + 模式以后;这个十的变化在相应 EOF3 部件主要被包含。然而, NCC_CGCM 预报结果是相当不同的,它在 1999 前揭示 ++ 模式并且 ++ 模式以后。同时,基于这些关键 SST 区域改进 NCC_CGCMs 预报精确性的概率被讨论,并且动态统计的联合预报计划为增加在东亚在夏天降水包含的十的变化的信息被构造。独立样品预报结果显示这个预报计划罐头有效地修改十的变化信息在东亚在夏天降水包含了的 NCC_CGCMs (特别在 30 敢琠敨攠癮 的区域吗?? | GONG ZhiQiang ZHAO JunHu FENG GuoLin CHOU JiFan | 2015 | Science China Earth Sciences2015，58，3： | 7 |
| 4 | Reconstruction of Conceptual Prediction Model for the Three Rainfall Patterns in the summer of eastern China under global warming显示文摘With the influence of global warming,the global climate has undergone significant inter-decadal variation since the late 1970s.Although El Nio-Southern Oscillation(ENSO)has been the strongest signal for predicting global climate inter-annual variability,its relation with the summer rainfall in China has significantly changed,and its indicative function on the summer rainfall in China has weakened.This has led to a significant decrease in the accuracy rate of early conceptual prediction models for the Three Rainfall Patterns in the summer of eastern China.On the basis of the difference analysis of atmospheric circulation system configuration in summer,as well as the interaction of ocean and atmospheric in previous winter between two phases,i.e.before and after the significant global warming(1951 to 1978 and 1979 to 2012,respectively),we concluded that(1)Under different inter-decadal backgrounds,the atmospheric circulations that impacted the Three Rainfall Patterns in the summer of eastern China showed consistency,but in the latter phase of the global warming,the Western Pacific Subtropical High(WPSH)was on the strong side,the position of which was in the south,and the blocking high in the Eurasia mid-high latitudes was active,while the polar vortex extended to the south,and meridional circulation intensified.This circulation background may have been conducive to the increase of the circulation frequency of Patterns II and III,and the decrease of the circulation frequency of Pattern I,thus leading to more Patterns II and III and fewer Pattern I in the summer rainfall of eastern China.(2)In the former phase,the corresponding previous winter SST fields of different rainfall patterns showed visible differences.The impact of ENSO on North Pacific Oscillation(NPO)was great,and the identification ability of which on Patterns I and II of summer rainfall was effective.In the latter phase,this identification ability decreased,while the impact of ENSO on the Pacific/North American(PNA)teleconnection pattern increased,and the identification ability of the PNA on Patterns II and III also increased.Based on the new inter-decadal climate background,this study reconstructs the conceptual prediction model for the Three Rainfall Patterns in summer of eastern China by using the previous winter PNA and the Eurasian(EU)teleconnection indexes.The fitting effect was satisfying,though it is necessary to be further tested. | ZHAO JunHu FENG GuoLin | 2014 | Science China Earth Sciences2014，57，12： | 7 |
| 5 | Rumen microbiome structure and metabolites activity in dairy cows with clinical and subclinical mastitis显示文摘Background:Due to the high prevalence and complex etiology,bovine mastitis(BM)is one of the most important diseases to compromise dairy cow health and milk quality.The shift in milk compositions has been widely investigated during mastitis,but recent studies suggested that gastrointestinal microorganism also has a crucial effect on the inflammation of other peripheral tissues and organs,including the mammary gland.However,research focused on the variation of rumen inner-environment during mastitis is still limited.Therefore,the ruminal microbial profiles,metabolites,and milk compositions in cows with different udder health conditions were compared in the present study.Furthermore,the correlations between udder health status and ruminal conditions were investigated.Based on the somatic cell counts(SCC),California mastitis test(CMT)parameters and clinical symptoms of mastitis,60 lactating Holstein dairy cows with similar body conditions(excepted for the udder health condition)were randomly divided into 3 groups(n=20 per group)including the healthy(H)group,the subclinical mastitis(SM)group and the clinical mastitis(CM)group.Lactation performance and rumen fermentation parameters were recorded.And rumen microbiota and metabolites were also analyzed via 16S rRNA amplicon sequencing and untargeted metabolomics,respectively.Results:As the degree of mastitis increased,rumen lactic acid(LA)(P<0.01),acetate,propionate,butyrate,valerate(P<0.001),and total volatile fatty acids(TVFAs)(P<0.01)concentrations were significantly decreased.In the rumen of CM cows,the significantly increased bacteria related to intestinal and oral inflammation,such as Lachnospiraceae(FDR-adjusted P=0.039),Moraxella(FDR-adjusted P=0.011)and Neisseriaceae(FDR-adjusted P=0.036),etc.,were accompanied by a significant increase in 12-oxo-20-dihydroxy-leukotriene B4(FDR-adjusted P=5.97×10^(−9))and 10beta-hydroxy-6beta-isobutyrylfuranoeremophilane(FDR-adjusted P=3.88×10^(−10)).Meanwhile,in the rumen of SM cows,the Ruminiclostridium_9(FDR-adjusted P=0.042)and Enterorhabdus(FDR-adjusted P=0.043)were increased along with increasing methenamine(FDR-adjusted P=6.95×10^(−6)),5-hydroxymethyl-2-furancarboxaldehyde(5-HMF)(FDR-adjusted P=2.02×10^(−6))and 6-methoxymellein(FDR-adjusted P=2.57×10^(−5)).The short-chain fatty acids(SCFAs)-producing bacteria and probiotics in rumen,including Prevoterotoella_1(FDRadjusted P=0.045)and Bifidobacterium(FDR-adjusted P=0.035),etc.,were significantly reduced,with decreasing 2-phenylbutyric acid(2-PBA)(FDR-adjusted P=4.37×10^(−6)).Conclusion:The results indicated that there was a significant shift in the ruminal microflora and metabolites associated with inflammation and immune responses during CM.Moreover,in the rumen of cows affected by SM,the relative abundance of several opportunistic pathogens and the level of metabolites which could produce antibacterial compounds or had a competitive inhibitory effect were all increased. | Yue Wang Xuemei Nan Yiguang Zhao Linshu Jiang Mengling Wang Hui Wang Fan Zhang Fuguang Xue Dengke Hua Jun Liu Junhu Yao Benhai Xiong | 2021 | Journal of Animal Science and Biotechnology2021，12，3： | 6 |
| 6 | High rumen degradable starch decreased goat milk fat via trans-10,cis-12 conjugated linoleic acid-mediated downregulation of lipogenesis genes,particularly,INSIG1显示文摘Background:Starch is an important substance that supplies energy to ruminants.To provide sufficient energy for high-yielding dairy ruminants,they are typically fed starch-enriched diets.However,starch-enriched diets have been proven to increase the risk of milk fat depression(MFD)in dairy cows.The starch present in ruminant diets could be divided into rumen-degradable starch(RDS)and rumen escaped starch(RES)according to their different degradation sites(rumen or intestine).Goats and cows have different sensitivities to MFD.Data regarding the potential roles of RDS in milk fat synthesis in the mammary tissue of dairy goats and in regulating the occurrence of MFD are limited.Results:Eighteen Guanzhong dairy goats(day in milk=185±12 d)with similar parity,weight,and milk yield were selected and randomly assigned to one of three groups(n=6),which were fed an LRDS diet(Low RDS=20.52%),MRDS diet(Medium RDS=22.15%),or HRDS diet(High RDS=24.88%)for 5 weeks.Compared with that of the LRDS group,the milk fat contents in the MRDS and HRDS groups significantly decreased.The yields of short-,mediumand long-chain fatty acids decreased in the HRDS group.Furthermore,increased RDS significantly decreased ruminal B.fibrisolvens and Pseudobutyrivibrio abundances and increased the trans-10,cis-12 conjugated linoleic acid(CLA)and trans-10 C18:1 contents in the rumen fluid.A multiomics study revealed that the HRDS diet affected mammary lipid metabolism down-regulation of ACSS2,MVD,AGPS,SCD5,FADS2,CERCAM,SC5D,HSD17B7,HSD17B12,ATM,TP53RK,GDF1 and LOC102177400.Remarkably,the significant decrease of INSIG1,whose expression was depressed by trans-10,cis-12 CLA,could reduce the activity of SREBP and,consequently,downregulate the downstream gene expression of SREBF1.Conclusions:HRDS-induced goat MFD resulted from the downregulation of genes involved in lipogenesis,particularly,INSIG1.Specifically,even though the total starch content and the concentrate-to-fiber ratio were the same as those of the high-RDS diet,the low and medium RDS diets did not cause MFD in lactating goats. | Lixin Zheng Shengru Wu Jing Shen Xiaoying Han Chunjia Jin Xiaodong Chen Shengguo Zhao Yangchun Cao Junhu Yao | 2020 | Journal of Animal Science and Biotechnology2020，11，3： | 4 |
| 7 | On the Relationship between the Winter Eurasian Teleconnection Pattern and the Following Summer Precipitation over China显示文摘The Eurasian teleconnection pattern(EU) is an important low-frequency pattern with well-known impacts on climate anomalies in Eurasia. The difference of low-level v-winds in several regions in the Eurasian mid–high latitudes is defined as the EU index(EUIV). In this study, the relationship between the winter EUIVand precipitation in the following summer over China is investigated. Results show that there is a significant positive(negative) correlation between the winter EUIVand the following summer precipitation over North China(the Yangtze River–Huaihe River basins). Meanwhile, an interdecadal variability exists in the interannual relationship, and the correlation has become significantly enhanced since the early 1980 s.Thus, the proposed EUIVmay have implications for the prediction of summer precipitation anomalies over China. In positive winter EUIVyears, three cyclonic circulation anomalies are observed—over the Ural Mountains, the Okhotsk Sea, and the subtropical western North Pacific. That is, the Ural blocking and Okhotsk blocking are inactive, zonal circulation prevails in the mid–high latitudes, and the western Pacific subtropical high tends to be weaker and locates to the north of its normal position in the following summer. This leads to above-normal moisture penetrating into the northern part of East China, and significant positive(negative) precipitation anomalies over North China(the Yangtze River–Huaihe River basins), and vice versa. Further examination shows that the SST anomalies over the Northwest Pacific and subtropical central North Pacific may both contribute to the formation of EUIV-related circulation anomalies over the western North Pacific. | Junhu ZHAO Liu YANG Bohui GU Jie YANG Guolin FENG | 2016 | Advances in Atmospheric Sciences2016，33，6： | 2 |
| 8 | Sulfur removal at high temperature during coal combustion in furnaces:a review显示文摘 | Jun Cheng Junhu Zhou Jianzhong Liu Zhijun Zhou Zhenyu Huang Xinyu Cao Xiang Zhao and Kefa Cen | | 0，，05： | 1 |
| 9 | An investigation on the rheological and sulfur-retention characteristics of desulfurizing coal water slurry with calcium-based additives显示文摘 | Liu Jianzhong Zhao Weidong Zhou Junhu | 2009 | Fuel Processing Technology2009，90，1： | 1 |
| 10 | Tracing enterococci persistence along a pork production chain from feed to food in China显示文摘The prevalence and transmission of vancomycin-resistant Enterococcus(VRE)in enterococci being as probiotics has been neglected in the scientific literature.The application of enterococci in feed,food and health products may cause VRE transmission through the food chain.This study evaluated phenotypic resistance of Enterococcus species to 20 antibiotics along a pork production chain from feed to food.It also assessed the genetic diversity of Enterococcus faecium isolates.A total of 510 samples(feed,n=70;swine manure,n=400;swine carcasses,n=20,and retail pork,n=20)were collected in Beijing,China.A total of 328 enterococci isolates with 275 E.faecium and 53 Enterococcus faecalis were identified using 16 S rRNA.Antimicrobial susceptibility to all enterococci isolates was conducted using the KeB method for 20 antibiotics from 9 categories.Multilocus sequence typing(MLST)was conducted on the E.faecium isolates to survey the dissemination of enterococci in the pig industry.The results showed that only 26 enterococci isolates were sensitive to the 20 antibiotics,while half of the isolates(164/328)had acquired multi-drug resistance.The resistant rate to furazolidone was 68.60%,followed by 42.99%to tetracycline.One vancomycin-resistant E.faecium isolates were isolated from feed origin and 2 from manure origin,with minimum inhibitory concentrations to vancomycin of 1,024,64,and 64 mg/mL,respectively.The MLST outcomes showed that the 275 E.faecium isolates belonged to 11 sequence types(ST)including ST40,ST60,ST94,ST160,ST178,ST296,ST361,ST695,ST726,ST812 and ST1014.The ST of the feedsourced VRE was ST1014,while the 2 manure-sourced VRE was ST69.ST1014 evolved from ST78,which was the dominant clonal complex in most cities of China,leading to the spreading of VRE.These findings revealed the potential safety hazards of commercial probiotic enterococci in China and showed that there is a risk of the VRE horizontally transferring from feed to food. | Jianfei Zhao Rui Liu Yanpeng Sun Xiaojun Yang Junhu Yao | 2022 | Animal Nutrition2022，，2： | 1 |
| 11 | Sulfur removal at high temperature during coal combustion in furnaces: a review显示文摘 | Jun Cheng Junhu Zhou Jianzhong Liu Zhijun Zhou Zhenyu Huang Xinyu Cao Xiang Zhao Kefa Cen | 2003 | Progress in Energy and Combustion Science2003，，5： | 1 |
| 12 | Sulfur removal at high temperature during coal combustion in furnaces: a review显示文摘 | Jun Cheng Junhu Zhou Jianzhong Liu Zhijun Zhou Zhenyu Huang Xinyu Cao Xiang Zhao Kefa Cen | 2003 | Progress in Energy and Combustion Science2003，，5： | 1 |
| 13 | Experimental study of acoustic agglomeration of coal-fired fly ash particles at low frequencies显示文摘 | Jianzhong Liu Guangxue Zhang Junhu Zhou Jie Wang Weidong Zhao Kefa Cen | 2009 | Powder Technology2009，，1： | 1 |
| 14 | Yak rumen microbiome elevates fiber degradation ability and alters rumen fermentation pattern to increase feed efficiency显示文摘Rumen microbes play an important role in ruminant energy supply and animal performance.Previous studies showed that yak(Bos grunniens)rumen microbiome and fermentation differ from other ruminants.However,little is understood about the features of the rumen microbiome that make yak adapted to their unique environmental and dietary conditions.This study was to investigate the rumen microbiome and metabolome to understand how yak adapt to the coarse forage and harsh environment in the Qinghai-Tibetan plateau.Nine female Qaidam yellow cattle(Bos taurus),9 dzomo(hybrids of cattle and yak)and 9 female plateau yak(B.grunniens),about 5 to 6 years old,were used in this study.Rumen fermentation parameters,fibrolytic enzyme activities,and rumen metataxonomic were determined.Then 18(6 samples per group)were selected for rumen metagenomic and metabolome analysis.Metataxonomic analysis revealed that the rumen microbiota was significantly different among plateau yak,Qaidam yellow cattle,and dzomo(P<0.05).Metagenomic analysis displayed a larger gene pool encoding a richer repertoire of carbohydrate-active enzymes in the rumen microbiome of plateau yak and dzomo than Qaidam yellow cattle(P<0.05).Some of the genes encoding glycoside hydrolases that mediate the digestion of cellulose and hemicellulose were significantly enriched in the rumen of plateau yak than Qaidam yellow cattle,but glycoside hydrolase 57 that primarily includes amylases was abundant in Qaidam yellow cattle(P<0.05).The rumen fermentation profile differed also,Qaidam yellow cattle having a higher molar proportion of acetate but a lower molar proportion of propionate than dzomo and plateau yak(P<0.05).Based on metabolomic analysis,rumen microbial metabolic pathways and metabolites were different.Differential metabolites are mainly amino acids,carboxylic acids,sugars,and bile acids.Changes in rumen microbial composition could explain the above results.The present study showed that the rumen microbiome of plateau yak helps its host to adapt to the Qinghai-Tibetan plateau.In particular,the plateau yak rumen microbiome has more enzymes genes involved in cellulase and hemicellulase than that of cattle,resulting higher fibrolytic enzyme activities inyak,further providing stronger fiber degradation function. | Congcong Zhao Lamei Wang Shanlin Ke Xinhua Chen Akos Kenez Wei Xu Dangdang Wang Fan Zhang Yong Li Zhanhong Cui Yu Qiao Jing Wang Wenjuan Sun Jianhua Zhao Junhu Yao Zhongtang Yu Yangchun Cao | 2022 | Animal Nutrition2022，，4： | 1 |
| 15 | Preparation ofdye waste-barium sulfate hybrid adsorbent and application inorganic wastewater treatment显示文摘 | ZHANG Junhu YAN Xiao ZHAO Danhua | 2010 | J Hazard Mater2010，175，123： | 1 |
| 16 | Dietary supplementation with inulin improves lactation performance and serum lipids by regulating the rumen microbiome and metabolome in dairy cows显示文摘This study investigated the effects of inulin on rumen fermentation parameters,ruminal microbiome and metabolites,as well as lactation performance and serum indexes in dairy cows.Sixteen Holstein dairy cows with similar body conditions were randomly divided into 2 groups(n=8 per group),with inulin addition at 0 and 200 g/d percow.The experiment lasted for 6 weeks,including a 1-week adaptation period and a 5-week treatment period.At the end of the experimental period,the milk,serum and rumen fluid were sampled and analyzed.The microbiome and metabolome in the rumen fluid were analyzed via 16 S rRNA sequencing and untargeted metabolomics,respectively.The results showed that supplementation with inulin(200 g/d per cow)increased the milk yield(P=0.001),milk protein(P=0.032),lacto se rate(P=0.004)and proportion of saturated fatty acids(SFA)in milk(P<0.001),but decreased the proportion of unsaturated fatty acids(USFA)(P=0.041).Rumen pH(P=0.040)and the concentration of NH3-N(P=0.024)were decreased;however,acetate(P<0.001),propionate(P=0.003),butyrate(P<0.001)and lactic acid(LA)(P=0.043)were increased.The total cholesterol(TC)(P=0.008)and triglycerides(TG)(P=0.01)in serum were also reduced.Additionally,inulin addition elevated the relative abundance of several beneficial symbiotic and short-chain fatty acid(SCFA)-producing bacteria,such as Muribaculaceae(false discovery rate[FDR]-adjusted P<0.01),Acetitomaculum(FDR-adjusted P=0.043),and Bu tyrivibrio(FDR-adjusted P=0.036),while elevating the levels of L-lysine(FDR-adjusted P=4.24×10^(-3)),L-proline(FDR-adjusted P=0.0158),and L-phenylalanine(FDRadj usted P=0.027).In contrast,several pathogens and ruminal bacteria abu ndant in high-fat diets,such as Escherichia-Sh igella(FD R-adj usted P=0.022),Erysipelo trichaceaeUCG-004(FD R-adjusted P<0.01)and RF39(FDR-adjusted P=0.042)were decreased along with the reduction of lysophosphatidylcholine(LysoPC)(18:1(9 Z))(FDR-adjusted P=1.03×10^(-3)),LysoPC(16:0)(FDR-adjusted P=0.0108),LysoPC(18:2(9 Z,12 Z))(FDRadjusted P=1.65×10^(-3))and 8-methylnonenoate.In conclusion,dietary inulin supplementation could increase the relative abundance of commensal microbiota and SCFA-producing bacteria,upregulate amino acidmetabolism and downregulate lipid metabolism in the rumen of dairy cows,which might further improve lactation performance and the level of serum lipids. | Yue Wang Xuemei Nan Yiguang Zhao Linshu Jiang Hui Wang Dengke Hua Fan Zhang Yapin Wang Jun Liu Junhu Yao Benhai Xiong | 2021 | Animal Nutrition2021，，4： | 1 |
| 17 | The Identification and Geological Significance of Fault Buried in the Gasikule Salt Lake in China based on the Multi-source Remote Sensing Data显示文摘The salinity of the salt lake is an important factor to evaluate whether it contains some mineral resources or not,the fault buried in the salt lake could control the abundance of the salinity.Therefore,it is of great geological importance to identify the fault buried in the salt lake.Taking the Gasikule Salt Lake in China for example,the paper established a new method to identify the fault buried in the salt lake based on the multi-source remote sensing data including Landsat TM,SPOT-5 and ASTER data.It includes the acquisition and selection of the multi-source remote sensing data,data preprocessing,lake waterfront extraction,spectrum extraction of brine with different salinity,salinity index construction,salinity separation,analysis of the abnormal salinity and identification of the fault buried in salt lake,temperature inversion of brine and the fault verification.As a result,the study identified an important fault buried in the east of the Gasikule Salt Lake that controls the highest salinity abnormal.Because the level of the salinity is positively correlated to the mineral abundance,the result provides the important reference to identify the water body rich in mineral resources in the salt lake. | WANG Junhu ZHAO Yingjun WU Ding LU Donghua | 2021 | Acta Geologica Sinica(English Edition)2021，95，3： | 1 |
| 18 | Synthesis of Iron-Carbide Nanoparticles:Identification of the Active Phase and Mechanism of Fe-Based Fischer-Tropsch Synthesis显示文摘Despite the extensive study of the Fe-based Fischer-Tropsch synthesis(FTS)over the past 90 years,its active phases and reaction mechanisms are still unclear due to the coexistence of metals,oxides,and carbide phases presented under realistic FTS reaction conditions and the complex reaction network involving CO activation,C-C coupling,and methane formation.To address these issues,we successfully synthesized a range of pure-phase iron and iron-carbide nanoparticles(Fe,Fe_(5)C_(2),Fe_(3)C,and Fe_(7)C_(3))for the first time.By using them as the ideal model catalysts on high-pressure transient experiments,we identified unambiguously that all the iron carbides are catalytically active in the FTS reaction while Fe_(5)C_(2) is the most active yet stable carbide phase,consistent with density functional theory(DFT)calculation results.The reaction mechanism and kinetics of Fe-based FTS were further explored on the basis of those model catalysts by means of transient high-pressure stepwise temperature-programmed surface reaction(STPSR)experiments and DFT calculations.Our work provides new insights into the active phase of iron carbides and corresponding FTS reaction mechanism,which is essential for better iron-based catalyst design for FTS reactions. | Huabo Zhao Jin-Xun Liu Ce Yang Siyu Yao Hai-Yan Su Zirui Gao Mei Dong Junhu Wang Alexandre I.Rykov Jianguo Wang Yanglong Hou Wei-Xue Li Ding Ma | 2021 | CCS Chemistry2021，3，11： | 1 |
| 19 | Identification of the E2F1-RAD51AP1 axis as a key factor in MGMT-methylated GBM TMZ resistance显示文摘Objective:Epidermal growth factor receptor variant III(EGFRvIII)is a constitutively-activated mutation of EGFR that contributes to the malignant progression of glioblastoma multiforme(GBM).Temozolomide(TMZ)is a standard chemotherapeutic for GBM,but TMZ treatment benefits are compromised by chemoresistance.This study aimed to elucidate the crucial mechanisms leading to EGFRvIII and TMZ resistance.Methods:CRISPR-Cas13a single-cell RNA-seq was performed to thoroughly mine EGFRvIII function in GBM.Western blot,realtime PCR,flow cytometry,and immunofluorescence were used to determine the chemoresistance role of E2F1 and RAD51-associated protein 1(RAD51AP1).Results:Bioinformatic analysis identified E2F1 as the key transcription factor in EGFRvIII-positive living cells.Bulk RNA-seq analysis revealed that E2F1 is a crucial transcription factor under TMZ treatment.Western blot suggested enhanced expression of E2F1 in EGFRvIII-positive and TMZ-treated glioma cells.Knockdown of E2F1 increased sensitivity to TMZ.Venn diagram profiling showed that RAD51AP1 is positively correlated with E2F1,mediates TMZ resistance,and has a potential E2F1 binding site on the promoter.Knockdown of RAD51AP1 enhanced the sensitivity of TMZ;however,overexpression of RAD51AP1 was not sufficient to cause chemotherapy resistance in glioma cells.Furthermore,RAD51AP1 did not impact TMZ sensitivity in GBM cells with high O6-methylguanine-DNA methyltransferase(MGMT)expression.The level of RAD51AP1 expression correlated with the survival rate in MGMT-methylated,but not MGMT-unmethylated TMZ-treated GBM patients.Conclusions:Our results suggest that E2F1 is a key transcription factor in EGFRvIII-positive glioma cells and quickly responds to TMZ treatment.RAD51AP1 was shown to be upregulated by E2F1 for DNA double strand break repair.Targeting RAD51AP1 could facilitate achieving an ideal therapeutic effect in MGMT-methylated GBM cells. | Junhu Zhou Fei Tong Jixing Zhao Xiaoteng Cui Yunfei Wang Guangxiu Wang Chunsheng Kang Xiaomin Liu Qixue Wang | 2023 | Cancer Biology & Medicine2023，20，5： | 0 |
| 20 | Systemic delivery of microRNA for treatment of brain ischemia显示文摘Brain ischemia is the second leading cause of death and the third leading cause of disability in the world.Systemic delivery of microRNA,a class of molecules that regulate the expression of cellular proteins associated with angiogenesis,cell growth,proliferation and differentiation,holds great promise for the treatment of brain ischemia.However,their therapeutic efficacy has been hampered by poor delivery efficiency of microRNA.We report herein a platform technology based on microRNA nanocapsules,which enables their effective delivery to the disease sites in the brain.Exemplified by microRNA-21,intravenous injection of the nanocapsules into a rat model of cerebral ischemia could effectively ameliorate the infarct volume,neurological deficit and histopathological severity. | Chaoyong Liu Jing Wen Dai Li Hongzhao Qi Lina Nih Jialin Zhu Duo Xu Yu Ren Shanshan Zhang Donglin Han Huanhuan Jia Junhu Zhou Meng Qin Jialing Wu Xubo Yuan Ji Liu Jin Zhao Chunsheng Kang Yunfeng Lu | 2021 | Nano Research2021，14，9： | 0 |
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