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| 1 | Ciliary parathyroid hormone signaling activates transforming growth factor-βto maintain intervertebral disc homeostasis during aging显示文摘Degenerative disc disease(DDD) is associated with intervertebral disc degeneration of spinal instability. Here, we report that the cilia of nucleus pulposus(NP) cells mediate mechanotransduction to maintain anabolic activity in the discs. We found that mechanical stress promotes transport of parathyroid hormone 1 receptor(PTH1 R) to the cilia and enhances parathyroid hormone(PTH) signaling in NP cells. PTH induces transcription of integrin α_vβ_6 to activate the transforming growth factor(TGF)-β-connective tissue growth factor(CCN2)-matrix proteins signaling cascade. Intermittent injection of PTH(iPTH) effectively attenuates disc degeneration of aged mice by direct signaling through NP cells, specifically improving intervertebral disc height and volume by increasing levels of TGF-β activity, CCN2, and aggrecan. PTH1 R is expressed in both mouse and human NP cells. Importantly,knockout PTH1 R or cilia in the NP cells results in significant disc degeneration and blunts the effect of PTH on attenuation of aged discs. Thus, mechanical stress-induced transport of PTH1 R to the cilia enhances PTH signaling, which helps maintain intervertebral disc homeostasis, particularly during aging, indicating therapeutic potential of iPTH for DDD. | Liwei Zheng Yong Cao Shuangfei Ni Huabin Qi Zemin Ling Xin Xu Xuenong Zou Tianding Wu Ruoxian Deng Bo Hu Bo Gao Hao Chen Yusheng Li Jianxi Zhu Francis Tintani Shadpour Demehri Amit Jain Khaled M.Kebaish Shenghui Liao Cheryle A.Séguin Janet L.Crane Mei Wan Hongbin Lu Paul D.Sponseller Lee H.RileyIII Xuedong Zhou Jianzhong Hu Xu Cao | 2018 | Bone Research2018，6，3： | 13 |
| 2 | Characteristics of Vibration and Sound Radiation of Metro Resilient Wheel显示文摘Resilient wheels are extensively used in urban rail transit, especially for tramway systems, owing to its advantages in noise reduction. A new type of resilient wheel for a metro is designed, and its characteristics of vibration and sound radiation, including the rolling noise of a resilient single wheel coupled with a track, are studied in this paper. A two-step research is presented. Firstly, laboratory experiments were conducted to obtain the vibration response of the designed resilient wheel under the radial excitation on its tread. Secondly, the rolling noise model of the resilient wheel coupled with a slab track used in a metro line is developed. The wheel model is based on the 3 D finite elementand boundary element methods and verified by using the experimental results obtained from the laboratory. The track vibration model is based on the wavenumber finite element method, and the track sound radiation is calculatedby using an e cient frequency-domain Rayleigh method. The interaction of the resilient wheel and the slab track is analyzed considering the measured wheel/rail roughness of the metro. The contribution of the resilient wheel to the reduction of wheel/rail system noise is analyzed. The results show that the resilient wheel can e ectively reduce the wheel/rail rolling noise by approximately 2 dB(A) to 3 dB(A), mainly because the radiated noise by the rail is reduced. In addition, the elastic modulus of the rubber has an important influence on the noise reduction of resilient wheels. | Xin Zhou Jian Han Yue Zhao Bing Wu Gong Cheng Shenghui Xu Xuesong Jin | 2019 | Chinese Journal of Mechanical Engineering2019，32，4： | 5 |
| 3 | The spatio-temporal variations of frost-free period in China from 1951 to 2012显示文摘The frost-free period(FFP)first frost date(FFD) and last frost date(LFD) have been regard as the important climate variables for agricultural production. Understanding the spatio-temporal variations of the FFPFFD and LFD is beneficial to reduce the harmful impacts of climate change on agricultural production and enhance the agricultural adaptation. This study examined daily minimum temperatures for 823 national-level meteorological stationscalculated the values of FFDLFD and FFP for station-specific and region-specific from 1951 to 2012estimated the gradients of linear regression for station-specific moving averages of FFDLFD and FFPand assessed station-specific time series of FFP and detected the abrupt change. The results as follows: at both the station level and the regional levelthe FFP across China decreases with the increase of latitude from south to northand with the increase of altitude from east to west generally. At the station levelthe inter-annual fluctuations of FFDLFD and FFP in south and west agricultural regions are greater than those in north and east. At the regional levelexcluding the QT regiontemporal changes of FFP are relatively small in both the low-latitude and the high-latitude regionsbut for the mid-latitude regions. According to the linear trend gradients of the moving average values of station-specific FFDLFD and FFPFFD was delayedLFD advancedand FFP extended gradually over the 80% of China. Furthermorethe change magnitudes for FFDLFD and FFP in the north and east agricultural regions are higher than that in the southern and western. Among the 659 station-specific time series of FFP examined by the Mann-Kendall test341 stationslocated mainly in the north regionhave one identifiable and significant abrupt change. And at the 341 stations with identified abrupt changesmost(57%) abrupt changes occurred during 1991–2012followed by the periods of 1981–1990(28%)1971–1980(12%)and 1951–1970(3%). The spatio-temporal variations of FFDLFD and FFP would provide important guidance to agricultural practices. | NING Xiaoju LIU Gangjun ZHANG Lijun QIN Xiaoyang ZHOU Shenghui QIN Yaochen | 2017 | Journal of Geographical Sciences2017，27，1： | 2 |
| 4 | Formation and evolution of intraplatform basin from the late Sinian to early Cambrian in Sichuan Basin,China显示文摘Based on the drilling,logging,seismic and outcrop data,the formation and evolution of the late Sinian to early Cambrian intraplatform basin in Sichuan Basin was studied.Through the analysis of the total residual thickness of Member 3+Member 4 of Dengying Formation,the total thickness of Maidiping Formation+Qiongzhusi Formation,and lithofacies characteristics of Maidiping Formation,the distribution of the intraplatform basin was defined and its controlling effect on natural gas distribution of Dengying Formation was revealed.The intraplatform basin was characterized by small total residual thickness of Member 3 and Member 4 of Dengying Formation,the large total thickness of Maidiping Formation and Qiongzhusi Formation,and the deep-water phosphorous-carbonaceous-siliceous mudstone in Maidiping Formation.Horizontally,the intraplatform basin was mainly distributed in Qingchuan-Ziyang-Yibin area,and experienced four evolution periods.During the period of Member 1 and Member 2 of Dengying Formation,the Sichuan Basinwas a rimmed carbonate platform with the well developed mounds and shoals on the platform margin and its interior.The intraplatform basin in western Sichuan Basin was initially formed due to the first episode of Tongwan movement.During the period of Member 3 and Member 4 of Dengying Formation,the depositional center was formed in Moxi,Changning and Zhengxiong area due to extension and rifting of the Upper Yangtze Craton,thereafter,the Sichuan Basin evolved into a rimmed carbonate platform with an intraplatform basin.The second episode of Tongwan movement between Sinian and Cambrian led to the overall uplift and exposure of Sichuan Basin characterized by weathering denudation at high position and downward fluid erosion at low position.During the period of Maidiping Formation,three lithofacies zones were developed horizontally,and the third episode of the Tongwan movement between Maidiping Formation and Qiongzhusi Formation modified the sedimentary geomorphology locally.During the period of Qiongzhusi Formation and Canglangpu Formation,continuous deposition made the Sichuan Basin evolve into a gentle siliciclastic ramp.The evolution of the intraplatform basin not only promoted formation of high-quality platform-margin dolomite reservoirs of Member 4 of Dengying Formation,but also controlled distribution of the high-quality source rocks of Maidiping Formation and Qiongzhusi Formation.The dolomite of Dengying Formation in the surrounding area of the intraplatform basin was the most favorable hydrocarbon exploration area because of its good source-reservoir configuration. | Hui Zhou Wei Li Baomin Zhang Jingjiang Liu Shenghui Deng Shiben Zhang Xiuqin Shan Jing Zhang Xiaobo Wang Hua Jiang | 2017 | Petroleum Research2017，2，1： | 2 |
| 5 | Nitrous oxide and nitric oxide emissions from an irrigated cotton field in Northern China显示文摘 | Chunyan Liu Xunhua Zheng Zaixing Zhou Shenghui Han Yinghong Wang Kai Wang Wangguo Liang Ming Li Deli Chen Zhiping Yang | 2010 | 2010 (1-2)2010，，1： | 2 |
| 6 | Synthesis and Characterization of a Silica-Based Drug Delivery System for Spinal Cord Injury Therapy显示文摘Acute inflammation is a central component in the progression of spinal cord injury(SCI).Anti-inflammatory drugs used in the clinic are often administered systemically at high doses,which can paradoxically increase inflammation and result in drug toxicity.A cluster-like mesoporous silica/arctigenin/CAQK composite(MSN-FC@ARCG)drug delivery system was designed to avoid systemic side effects of high-dose therapy by enabling site-specific drug delivery to the spinal cord.In this nanosystem,mesoporous silica was modified with the FITC fluorescent molecule and CAQK peptides that target brain injury and SCI sites.The size of the nanocarrier was kept at approximately 100 nm to enable penetration of the blood–brain barrier.Arctigenin,a Chinese herbal medicine,was loaded into the nanosystem to reduce inflammation.The in vivo results showed that MSN-FC@ARC-G could attenuate inflammation at the injury site.Behavior and morphology experiments suggested that MSN-FC@ARC-G could diminish local microenvironment damage,especially reducing the expression of interleukin-17(IL-17) and IL-17-related inflammatory factors,inhibiting the activation of astrocytes,thus protecting neurons and accelerating the recovery of SCI.Our study demonstrated that this novel,silica-based drug delivery system has promising potential for clinical application in SCI therapy. | Guodong Sun Shenghui Zeng Xu Liu Haishan Shi Renwen Zhang Baocheng Wang Changren Zhou Tao Yu | 2019 | Nano-Micro Letters2019，11，2： | 1 |
| 7 | Studies on photoprotective mechanism of desert plant Reaumuria soongorica under progressive soil drying显示文摘Reaumuria soongorica (pall.) Maxim, a perennial desert semi-shrub, is widely found in semi-arid areas of China. It shows strong tolerance to drought, high temperature and intense radiation in its natural habitat. In the present study, photoprotective mechanism of R. soongorica was investigated by analyzing diurnal variations of gas exchange and chlorophyll fluorecence parameters during progressive soil drying. The results show that leaf water potential of R. soongorica decreased when the soil water content dropped. Diurnal patterns of net photosynthetic rate (Pn) changed from 'two peaks' to 'one peak' under drought stress, and the leaf water use efficiency (WUE) increased under moderate drought and declined under severe drought. Pn , the primary maximum photochemical efficiency of PSII (Fv/Fm) and the quantum efficiency of non-cyclic electron transport of PSII (ΦPSII) decreased obviously at noon, and showed a photoinhibition phenomenon. But, the non-photochemical quenching of fluorescence (NPQ) soon reached its maximum in the day and then remained almost at the high level until 17:00, indicating that the xanthophylls cycle-dependent thermal energy dissipation played an important role. Diurnal variations of the original chlorophyll fluorescence (Fo) increased at first and then decreased. The increased value of F o under drought stress indicates that there was a reversible inactivation of PSII reaction center. These results indicate that the photoprotective mechanism in R. soongorica was the photoinhibition by using both the xanthophylls cycle-dependent thermal energy dissipation and the reversible inactivation of PSII reaction center under drought stress. | YuBing Liu MeiLing Liu HuiJuan Tan Jin Wang ShengHui Zhou | 2010 | Research in Cold and Arid Regions2010，2，3： | 1 |
| 8 | Surface-modification of indium tin oxide nanoparticles with titanium dioxide by a nonaqueous process and its photocatalytic properties 显示文摘 | Rongjun Pan Shenghui Pan Juying Zhou Yucheng Wu | 2009 | Science2009，255，6： | 1 |
| 9 | Increased CD133^+ cell infiltration in the rat brain following fluid percussion injury显示文摘The prominin-1/CD133 epitope is expressed in undifferentiated cells. Studies have reported that craniocerebral trauma in animal models of fluid percussion injury induces production of a specific stem cell subgroup. It has been hypothesized that fluid percussion injury induces CD133+ cell infiltration in the brain tissue. The present study established a traumatic brain injury model through fluid percussion injury. Immunohistochemical staining showed significantly increased CD133 antigen expression in the rat brain following injury. CD133+ cells were mainly distributed in hippocampal CA1-3 regions, as well as the dentate gyrus and hilus, of the lesioned hemisphere. Occasional cells were also detected in the cortex. In addition, reverse transcription-PCR revealed that no change in CD133 mRNA expression in injured brain tissue. These results suggested that fluid percussion injury induced CD133 antigen expression in the brain tissues as a result of conformational epitope changes, but not transcriptional expression. | Ming Wei Ziwei Zhou Shenghui Li Chengwei Jing Dashi Zhi Jianning Zhang | 2012 | Neural Regeneration Research2012，7，4： | 1 |
| 10 | Development of P genome-specific SNPs and their application in tracing Agropyron cristatum introgressions in common wheat显示文摘As an important wild relative of wheat, Agropyron cristatum has been successfully used for wheat improvement. Currently, a few useful agronomic traits of A. cristatum, such as high grain number per spike and resistance to diseases, have been transferred into common wheat.However, the effective detection of small A. cristatum segmental introgressions in common wheat is still difficult. The objective of this study was to identify A. cristatum-specific single nucleotide polymorphisms(SNPs) for the detection of small alien segments in wheat. The transcriptome sequences of A. cristatum were aligned against wheat coding DNA sequences(CDS) for SNP calling. As a result, we discovered a total of 167,613 putative SNPs specific to the P genome of A. cristatum compared with the common wheat genomes. Among 230 selected SNPs with functional annotations related to inflorescence development and stress resistance,68 were validated as P genome-specific SNPs in multiple wheat backgrounds using Kompetitive Allele Specific PCR(KASP) assays. Among them, 55 SNPs were assigned to six homoeologous groups of the P genome using wheat-A. cristatum addition lines, and 6 P-specific SNP markers were further physically mapped on different segments of chromosome 6 P in 6 P translocation lines. The P genome-specific SNPs were also validated by Sanger sequencing and used to detect the P chromatin in wheat-A. cristatum cryptic introgression lines. Two SNP markers(Unigene20217-182 and Unigene20307-1420) were detected in two wheat-A. cristatum introgression lines that showed enhanced grain number per spike and high resistance to powdery mildew. Together, the developed P genome-specific SNP markers will accelerate the detection of large numbers of wheat-A. cristatum derivatives and will be helpful for marker-assisted transfer of desirable traits from A. cristatum into adapted wheat cultivars in wheat breeding programs. | Huihui Ma Jinpeng Zhang Jing Zhang Shenghui Zhou Haiming Han Weihua Liu Xinming Yang Xiuquan Li Lihui Li | 2019 | The Crop Journal2019，7，2： | 1 |
| 11 | Surfacemodification of indium tin oxide nanoparticles with titaniumdioxide by a nonaqueous process and its photocatalytic properties显示文摘 | Rongjun Pan Shenghui Pan Juying Zhou | | 0，，06： | 1 |
| 12 | Synthesis and characterization of trivalent metal porphyrin with NCS liand for application in dye-sensitized solar cells显示文摘 | XIANG Na ZHOU Weiping JIANG Shenghui | 2011 | Sol Energy Mater Sol Cells2011，95，： | 1 |
| 13 | Nitrous oxide and nitric oxide emissions from an irrigated cotton field in Northern China显示文摘 | Chunyan Liu Xunhua Zheng Zaixing Zhou Shenghui Han Yinghong Wang Kai Wang Wangguo Liang Ming Li Deli Chen Zhiping Yang | 2010 | Plant and Soil . 2010 (1-2)2010，，1： | 1 |
| 14 | Introgression of chromosome 6PL terminal segment from Agropyron cristatum to increase both grain number and grain weight in wheat显示文摘Agropyron cristatum(2n=4x=28,PPPP),which harbours many high-yield and disease-resistance genes,is a promising donor for wheat improvement.Narrow genetic diversity and the trade-off between grain weight and grain number have become bottlenecks for increasing grain yield in wheat.In this study,a novel translocation line,WAT650l,was derived from the chromosome 6P addition line 4844–12,which can simultaneously increase both grain number per spike(GNS)and thousand-grain weight(TGW).Cytological analysis and molecular marker analysis revealed that WAT650l was a 5BL.5BS-6PL(bin 12–17)translocation line.Assessment of agronomic traits and analysis of the BC4F2 and BC5F2 populations suggested that the 6PL terminal chromosome segment in WAT650l resulted in increased grain number per spike(average increased by 14.07 grains),thousand-grain weight(average increased by 4.31 g),flag leaf length,plant height,spikelet number per spike and kernel number per spikelet during the two growing seasons of 2020–2021 and 2021–2022.Additionally,the increased GNS locus and high-TGW locus of WAT650l were mapped to the bins 16–17 and 12–13,respectively,on chromosome 6PL by genetic population analysis of three translocation lines.In summary,we provide a valuable germplasm resource for broadening the genetic base of wheat and overcoming the negative relationship between GNS and TGW in wheat breeding. | Yida Lin Shenghui Zhou Xuezhong Liang Bing Han Junli Yang Baojin Guo Jinpeng Zhang Haiming Han Weihua Liu Xinming Yang Xiuquan Li Lihui Li | 2023 | The Crop Journal2023，11，3： | 1 |
| 15 | Sequencing and comparative analyses of Aegilops tauschii chromosome arm 3DS reveal rapid evolution of Triticeae genomes显示文摘Bread wheat(Triticum aestivum,AABBDD) is an allohexaploid species derived from two rounds of interspecific hybridizations.A high-quality genome sequence assembly of diploid Aegilops tauschii,the donor of the wheat D genome,will provide a useful platform to study polyploid wheat evolution.A combined approach of BAC pooling and next-generation sequencing technology was employed to sequence the minimum tiling path(MTP) of 3176 BAC clones from the short arm of Ae.tauschii chromosome 3(At3DS).The final assembly of 135 super-scaffolds with an N50 of 4.2 Mb was used to build a247-Mb pseudomolecule with a total of 2222 predicted protein-coding genes.Compared with the orthologous regions of rice,Brachypodium,and sorghum,At3 DS contains 38.67%more genes.In comparison to At3 DS,the short arm sequence of wheat chromosome 3B(Ta3BS) is 95-Mb large in size,which is primarily due to the expansion of the non-centromeric region,suggesting that transposable element(TE) bursts in Ta3 B likely occurred there.Also,the size increase is accompanied by a proportional increase in gene number in Ta3 BS.We found that in the sequence of short arm of wheat chromosome 3D(Ta3DS),there was only less than 0.27%gene loss compared to At3 DS.Our study reveals divergent evolution of grass genomes and provides new insights into sequence changes in the polyploid wheat genome. | Jingzhong Xie Naxin Huo Shenghui Zhou Yi Wang Guanghao Guo Karin R.Deal Shuhong Ouyang Yong Liang Zhenzhong Wang Lichan Xiao Tingting Zhu Tiezhu Hu Vijay Tiwari Jianwei Zhang Hongxia Li Zhongfu Ni Yingyin Yao Huiru Peng Shengli Zhang Olin D.Anderson Patrick E.McGuire Jan Dvorak Ming-Cheng Luo Zhiyong Liu Yong Q.Gu Qixin Sun | 2017 | Journal of Genetics and Genomics2017，44，1： | 1 |
| 16 | Metal salts with highly electronegative cations as efficient catalysts for the liquid-phase nitration of benzene by NO2 to nitrobenzene显示文摘有高度阴电的阳离子的金属盐被用来有效地在没有溶剂的条件下面由没有 2 催化苯的液体阶段硝代到硝基苯。几盐包括 FeCl 3, ZrCl 4, AlCl 3, CuCl 2, NiCl 2, ZnCl 2, MnCl 2, Fe (没有 3)39H2 O ,双性人(没有 3)35H2 O , Zr (没有 3)45H2 O , Cu (没有 3)26H2 O , Ni (没有 3)26H2 O , Zn (没有 3)26H2 O , Fe 2(SO4)3,和 CuSO 4是 examin 到硝基苯的苯变换和选择两个都是超过 99% 。另外,金属 counterion 和在盐的水水合物的存在影响催化活动,这被决定。这个方法简单、有效并且可以有潜在的工业申请前景。 | Shenghui Zhou Kuiyi You Zhengming Yi Pingle Liu Hean Luo | 2017 | Frontiers of Chemical Science and Engineering2017，11，2： | 0 |
| 17 | Crystal structures of main proteases of SARS-CoV-2 variants bound to a benzothiazole-based inhibitor显示文摘Main protease(Mpro)serves as an indispensable factor in the life cycle of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)as well as its constantly emerging variants and is therefore considered an attractive target for antiviral drug development.Benzothiazole-based inhibitors targeting Mpro have recently been investigated by several groups and proven to be promising leads for coronaviral drug development.In the present study,we determine the crystal structures of a benzothiazole-based inhibitor,YH-53,bound to Mpro mutants from SARS-CoV-2 variants of concern(VOCs)or variants of interest(VOIs),including K90R(Beta,B.1.351),G15S(Lambda,C.37),Y54C(Delta,AY.4),M49I(Omicron,BA.5)and P132H(Omicron,B.1.1.529).The structures show that the benzothiazole group in YH-53 forms a C-S covalent bond with the sulfur atom of catalytic residue Cys145 in SARSCoV-2 Mpro mutants.Structural analysis reveals the key molecular determinants necessary for interaction and illustrates the binding mode of YH-53 to these mutant Mpros.In conclusion,structural insights from this study offer more information to develop benzothiazole-based drugs that are broader spectrum,more effective and safer. | Jiqing Luo Weiwei Wang Haihai Jiang Wenwen Li Pei Zeng Jie Wang Xuelan Zhou Xiaofang Zou Shenghui Chen Qisheng Wang Jin Zhang Jian Li | 2023 | Acta Biochimica et Biophysica Sinica2023，55，8： | 0 |
| 18 | Co-construction of advanced sulfur host by implanting titanium carbide into Aspergillus niger spore carbon显示文摘It is of great importance to directionally construct advanced carbon host to achieve high-performance carbon/sulfur cathodes for lithium sulfur batteries(LSBs).Herein,we report a unique hollow pumpkinlike carbon with notable rich-wrinkle microstructure and intrinsically dual doping with N&P elements via a facile annealing process of Aspergillus niger spore.Furthermore,highly conductive polar absorbents,Ti C nanoparticles,are in situ implanted into the above Aspergillus niger spore carbon(ANSC)by carbothermal reaction,accordingly forming high-performance ANSC/TiC composite host for sulfur.Impressively,TiC nanoparticles play dual roles of not only pore formation in ANSC matrix but also enhancement of chemical absorption with polysulfides.With the positive synergistic effect between N&P co-doped ANSC matrix and Ti C polar absorbent,the designed ANSC/Ti C-S cathodes show unique advantages including larger accommodation space for sulfur,higher surface area,enhanced conductivity and better chemical absorption with soluble polysulfide intermediates.Consequently,the ANSC/Ti C-S cathodes are endowed with good rate performance(496 mAh/g at 0.5 C)and enhanced long-term cycling stability(736 mAh/g with a capacity retention of 78.8%at 0.1 C after 100 cycles).Our research opens a new door to controllably design advanced composite cathodes from microorganisms for application in lithium sulfur batteries. | Rongfan Zhou Shenghui Shen Yu Zhong Ping Liu Yongqi Zhang Lingjie Zhang Xiuli Wang Xinhui Xia Jiangping Tu | 2022 | Chinese Chemical Letters2022，33，8： | 0 |
| 19 | Seismic performance analysis of underground structures based on random field model of soil mechanical parameters显示文摘Soils with spatial variability are the product of natural history.The mechanical properties tested by soil samples from boreholes in the same soil layer may be different.Underground structure service in surrounding soils,their seismic response is controlled by the deformation of the surrounding soils.The variability of soil mechanical parameters was not considered in the current research on the seismic response of underground structures.Therefore,a random field model was established to describe the spatial variability of surrounding soils based on the random field theory.Then the seismic response of underground structures in the random field was simulated based on the time-domain explicit global FEM analysis,and the soil mechanical parameters and earthquake intensity influencing the seismic response of surrounding soils and underground structures were studied.Numerical results presented that,the randomness of soil parameters does not change the plastic deformation mode of surrounding soils significantly.The variation coefficients of inter-story deformation of structures and lateral deformation of columns are much smaller than that of mechanical parameters,and the randomness of soil parameters has no obvious effect on the structural deformation response. | Chao Ma Shenghui Zhou Jingwei Chi | 2022 | Earthquake Research Advances2022，2，4： | 0 |
| 20 | Chromosome 5P of Agropyron cristatum induces chromosomal translocation by disturbing homologous chromosome pairing in a common wheat background显示文摘Wide hybridization is a strategy for broadening the genetic basis of wheat. Because an efficient method for inducing wheat–alien chromosome translocations will allow producing useful germplasm, it is desirable to discover new genes that induce chromosomal variation. In this study, chromosome 5P from A.cristatum was shown to induce many types of chromosomal structural variation in a common wheat background, including nonhomoeologous chromosome translocations, as revealed by genomic in situ hybridization, fluorescence in situ hybridization, and DNA marker analysis. Aberrant meiosis was associated with chromosomal structural variation, and aberrant meiotic behavior was observed in wheat–A.cristatum 5P monosomic and disomic addition lines, suggesting that the effect of chromosome 5P was independent of the number of chromosome 5P copies. Chromosome 5P disturbed homologous chromosome pairing at pachytene stage in a common wheat background, resulting in a high frequency of univalent formation and reduced crossing over. Thirteen genes involved in DNA repair or chromatin remodeling, including RAD52-like and MSH6 genes, were differentially expressed(upregulated) in wheat–A. cristatum 5P addition lines according to transcriptome analysis, implicating chromosome 5P in the process of meiotic double-strand break repair. These findings provide a new, efficient tool for inducing wheat–alien chromosome translocations and producing new germplasm. | Haiming Han Xinyuan Ma Zhen Wang Kai Qi Wenjing Yang Weihua Liu Jinpeng Zhang Shenghui Zhou Yuqing Lu Xinming Yang Xiuquan Li Lihui Li | 2023 | The Crop Journal2023，11，1： | 0 |
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