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| 1 | Natural Variation in CCD4 Promoter Underpins Species-Specific Evolution of Red Coloration in Citrus Peel显示文摘Carotenoids and apocarotenoids act as phytohormones and volatile precursors that influence plant development and confer aesthetic and nutritional value critical to consumer preference.Citrus fruits display considerable natural variation in carotenoid and apocarotenoid pigments.In this study,using an integrated genetic approach we revealed that a 5;c/s-regulatory change at CCD4b encoding CAROTENOID CLEAVAGE DIOXYGENASE 4b is a major genetic determinant of natural variation in C3 0 apocarotenoids responsible for red coloration of citrus peel.Functional analyses demonstrated that in addition the known role in synthesizing 3-citraurin,CCD4b is also responsible for the production of another important C3 0 apocarotenoid pigment,p-citraurinene.Furthermore,analyses of the CCD4b promoter and transcripts from various citrus germplasm accessions established a tight correlation between the presence of a putative 5'c/s-regulatory enhancer within an MITE transposon and the enhanced allelic expression of CCD4b in C3 0 apocarotenoid-rich red-peeled accessions.Phylogenetic analysis provided further evidence that functional diversification of CCD4b and naturally occurring variation of the CCD4b promoter resulted in the stepwise evolution of red peels in mandarins and their hybrids.Taken together,our findings provide new insights into the genetic and evolutionary basis of apocarotenoid diversity in plants,and would facilitate breeding efforts that aim to improve the nutritional and aesthetic value of citrus and perhaps other fruit crops. | Xiongjie Zheng Kaijie Zhu Quan Sun Weiyi Zhang Xia Wang Hongbo Cao Meilian Tan Zongzhou Xie Yunliu Zeng Junli Ye Lijun Chai Qiang Xu Zhiyong Pan Shunyuan Xiao Paul D.Fraser Xiuxin Deng | 2019 | Molecular Plant2019，12，9： | 18 |
| 2 | Genome-wide Characterization of cis-acting Elements in the Promoters of Key Carotenoid Pathway Genes from the Main Species of Genus Citrus显示文摘Carotenoids are indispensable for both human health and plant survival.Citrus,is one of the fruit crops richest in carotenoid compounds,with approximately 115 kinds of carotenoids;tremendous diversity in carotenoids composition and concentration exists among various species,showing different colors from nearly white to crimson.The carotenoid biosynthetic pathway and the key carotenogenic genes have been identified in citrus;however,the underlying regulatory mechanisms remain unclear.In this study,among the main species of genus Citrus(primitive,wild,and cultivated),we detected carotenoids in flavedo using High-Performance Liquid Chromatography,and analyzed variations in cis-acting elements in the promoters of key carotenoid pathway genes.Intriguingly,both carotenoid composition and content were generally increased during the evolution of citrus,and the corresponding variations in the promoters were identified,including the gain or loss of critical environmental stress-responsive elements and hormone-responsive elements,which are closely associated with carotenoid enhancement.In addition,pummelo has the most heat-responsive elements,but the Mangshan mandarin does not have this element in the promoters of PSY,which is highly related to their geographical origin and indicate that temperature is a critical environmental signal influencing carotenoid accumulation.Moreover,the abscisic acid-responsive motif was rich in almost all the seven species,but the ethylene-responsive motif was deficient,which demystified the unique phytohormone regulation mechanism of carotenoid accumulation in citrus.Overall,our study provides new insights into the molecular regulatory mechanism of carotenoid enhancement in the evolution of citrus,which can facilitate breeding and cultivation efforts to improve the nutritional quality and esthetic value in citrus and hopefully other fruit crops. | Kaijie Zhu Qingjiang Wu Yue Huang Junli Ye Qiang Xu Xiuxin Deng | 2020 | Horticultural Plant Journal2020，6，6： | 8 |
| 3 | Generation of 33 fs pulses directly from a Kerr-lens mode-locked Yb:CaYAlO4 laser显示文摘We experimentally demonstrated a diode-pumped Kerr-lens mode-locked femtosecond laser with a Yb:CaYAlO4 crystal as the gain medium. Pulse duration as short as 33 fs was obtained directly from the oscillator at a repetition rate of 115 MHz. The central wavelength was at 1059 nm with a spectral bandwidth of 49 nm. These are, to the bestof our knowledge, the shortest pulses generated from a Yb:CaYAlO4 oscillator. | Ziye Gao Jiangfeng Zhu Junli Wang Zhiyi Wei Xiaodong Xu Lihe Zheng Liangbi Su Jun Xu | 2015 | Photonics Research2015，3，6： | 8 |
| 4 | Screen-imaging guidance using a modified portable video macroscope for middle cerebral artery occlusion显示文摘The use of operating microscopes is limited by the focal length.Surgeons using these instruments cannot simultaneously view and access the surgical field and must choose one or the other.The longer focal length (more than 1 000 mm) of an operating telescope permits a position away from the operating field,above the surgeon and out of the field of view.This gives the telescope an advantage over an operating microscope.We developed a telescopic system using screen-imaging guidance and a modified portable video macroscope constructed from a Computar MLH-10 × macro lens,a DFK-21AU04 USB CCD Camera and a Dell laptop computer as monitor screen.This system was used to establish a middle cerebral artery occlusion model in rats.Results showed that magnification of the modified portable video macroscope was appropriate (5-20 ×) even though the Computar MLH-10 × macro lens was placed 800 mm away from the operating field rather than at the specified working distance of 152.4 mm with a zoom of 1-40 ×.The screen-imaging telescopic technique was clear,life-like,stereoscopic and matched the actual operation.Screen-imaging guidance led to an accurate,smooth,minimally invasive and comparatively easy surgical procedure.Success rate of the model establishment evaluated by neurological function using the modified neurological score system was 74.07%.There was no significant difference in model establishment time,sensorimotor deficit and infarct volume percentage.Our findings indicate that the telescopic lens is effective in the screen surgical operation mode referred to as 'long distance observation and short distance operation' and that screen-imaging guidance using an modified portable video macroscope can be utilized for the establishment of a middle cerebral artery occlusion model and micro-neurosurgery. | Xingbao Zhu Junli Luo Yun Liu Guolong Chen Song Liu Qiangjin Ruan Xunding Deng Dianchun Wang Quanshui Fan Xinghua Pan | 2012 | Neural Regeneration Research2012，7，12： | 6 |
| 5 | Microarray Expression Profiling of Postharvest Ponkan Mandarin (Citrus reticulata) Fruit under Cold Storage Reveals Regulatory Gene Candidates and Implications on Soluble Sugars Metabolism显示文摘Low temperature storage is widely applied to maintain citrus postharvest fruit quality.In this study,the transcriptional and metabolic changes in the pulp tissue of Citrus reticulata Blanco cv.'Ponkan' were studied for three successive months under cold storage by Affymetrix Citrus GeneChip and gas chromatography,respectively.As many as 2 161 differentially expressed transcripts were identified based on the bayesian hierarchical model.The statistical analysis of gene ontology revealed that defense/stress-related genes were induced quickly,while autophagy-related genes were overrepresented in the late sampling stages,suggesting that the functional shift may coincide with the subsequent steps of chilling development.We further classified the potential regulatory components and concluded that ethylene may play the crucial role in chilling development in this non-climacteric fruit.To cope with complex events,53 upregulated transcription factors represented regulatory candidates.Within these,the AP2-EREBP,C2H2 and AS2 gene family were overrepresented.Cold storage also causes alterations in various metabolic pathways;a keen interest is paid in deciphering expression changes of soluble sugar genes as increased evidence that soluble sugars act as both osmolytes and metabolite signal molecules.Our results will likely facilitate further studies in this field with promising genetic candidates during chilling. | Andan Zhu Wenyun Li Junli Ye Xiaohua Sun Yuduan Ding Yunjiang Cheng Xiuxin Deng | 2011 | Journal of Integrative Plant Biology2011，53，5： | 3 |
| 6 | 2020 Roadmap on two-dimensional nanomaterials for environmental catalysis显示文摘Environmental catalysis has drawn a great deal ofattention due to its clean ways to produce useful chemicals or carry out some chemical processes.Photocatalysis and electrocatalysis play important roles in these fields.They can decompose and remove organic pollutants from the aqueous environment,and prepare some fine chemicals.Moreover,they also can carry out some important reactions,such as 02 reduction reaction(ORR),O2 evolution reaction(OER),H2 evolution reaction(HER),CO2 reduction reaction(C02 RR),and N2 fixation(NRR).For catalytic reactions,it is the key to develop high-performance catalysts to meet the demand fortargeted reactions.In recentyears,two-dimensional(2 D) materials have attracted great interest in environmental catalysis due to their unique layered structures,which offer us to make use of their electronic and structural characteristics.Great progress has been made so far,including graphene,black phosphorus,oxides,layered double hydroxides(LDHs),chalcogenides,bismuth-based layered compounds,MXenes,metal organic frameworks(MOFs),covalent organic frameworks(COFs),and others.This content drives us to invite many famous groups in these fields to write the roadmap on two-dimensional nanomaterials for environmental catalysis.We hope that this roadmap can give the useful guidance to researchers in future researches,and provide the research directions. | Yulu Yang Mingguang Wu Xingwang Zhu Hui Xu Si Ma Yongfeng Zhi Hong Xia Xiaoming Liu Jun Pan Jie-Yinn Tang Siang-Piao Chai Leonardo Palmisano Francesco Parrino Junli Liu Jianzhong Ma Ze-Lin Wang Ling Tan Yu-Fei Zhao Yu-Fei Song Pardeep Singh Pankaj Raizada Deli Jiang Di Li R.A.Geioushy Jizhen Ma Jintao Zhang Song Hu Rongjuan Feng Gang Liu Minghua Liu Zhenhua Li Mingfei Shao Neng Li Jiahe Peng Wee-Jun Ong Nikolay Kornienko Zhenyu Xing Xiujun Fan Jianmin Ma | 2019 | Chinese Chemical Letters2019，30，12： | 2 |
| 7 | Satellite RNA-mediated Reduction of Cucumber Mosaic Virus Genomic RNAs Accumulation in Nicotiana tabacum显示文摘卫星 RNA (satRNAs ) 是防碍助手病毒的致病的分子的寄生虫。在这研究,黄瓜马赛克的相对累积病毒(CMV )-Fny genomic RNA 与或没有 satRNAs 是 real-timeRT-PCR 分析的份量上。结果证明 satRs 显然由压抑 CMV-Fny genomic RNA 的累积在 Nicotianatabacum 上稀释了 CMV-Fny 的症状,测试同样开的读物框架。CMV-Fny 1a, 2a, 2b, 3a,和 CP 基因的 Theaccumulation 比有 satRsadded (CMV-Fsat ) 的 CMV-Fny 的高得多,在不同接种时间。 CMV-Fny Δ2 b ,在 2a 基因的C终端的完全的 2b 基因和 41amino 酸在被删除,在 N.tabacum 幼苗上引起了仅仅细微马赛克效果,类似于 CMV-Fsat 的,但是到 CMV-Fny Δ2 b 的 satRs 的增加在 CMV-Fny Δ2 b genomic RNA 的累积显示出进一步的减少。我们的结果显示由增加 satRs 或删除 2b 基因, CMV 的变细由于 CMVgenomic RNA,和在 N 的 CMV genomic RNA 累积的那调停 satRNA 的减小的低累积。tabacum 是可能与 2b 基因有关。 | Qiansheng LIAO Liping ZHU Zhiyou DU Rong ZENG Junli FENG Jishuang CHEN | 2007 | Acta Biochimica et Biophysica Sinica2007，39，3： | 2 |
| 8 | Antimicrobial effect and membrane-active mechanism of tea polyphenols against Serratia marcescens显示文摘 | Shumin Yi Wei Wang Fengling Bai Junli Zhu Jianrong Li Xuepeng Li Yongxia Xu Tong Sun Yutang He | 2014 | World Journal of Microbiology and Biotechnology2014，，2： | 2 |
| 9 | Multiomics-based dissection of citrus flavonoid metabolism using a Citrus reticulata × Poncirus trifoliata population显示文摘Deciphering the genetic basis of plant secondary metabolism will provide useful insights for genetic improvement and enhance our fundamental understanding of plant biological processes.Although citrus plants are among the most important fruit crops worldwide,the genetic basis of secondary metabolism in these plants is largely unknown.Here,we use a high-density linkage map to dissect large-scale flavonoid metabolic traits measured in different tissues(young leaf,old leaf,mature pericarp,and mature pulp)of an F_(1) pseudo-testcross citrus population.We detected 80 flavonoids in this population and identified 138 quantitative trait loci(QTLs)for 57 flavonoids in these four tissues.Based on transcriptional profiling and functional annotation,twenty-one candidate genes were identified,and one gene encoding flavanone 3-hydroxylase(F3H)was functionally verified to result in naturally occurring variation in dihydrokaempferol content through genetic variations in its promoter and coding regions.The abundant data resources collected for diverse citrus germplasms here lay the foundation for complete characterization of the citrus flavonoid biosynthetic pathway and will thereby promote efficient utilization of metabolites in citrus quality improvement. | Jiaolin Mou Zhehui Zhang Haiji Qiu Yang Lu Xiang Zhu Ziquan Fan Qinghua Zhang Junli Ye Alisdair R.Fernie Yunjiang Cheng Xiuxin Deng Weiwei Wen | 2021 | Horticulture Research2021，8，1： | 2 |
| 10 | The Application Analysis of Information Dissemination of Agriculturerelated related Micro - blogs in Chinal显示文摘 | Junli Zhang Xuefang Zhu | 2012 | 6(13) :305 -3132012，6，13： | 1 |
| 11 | Aquatic products processin~ industry in China: Challenges and outlook显示文摘 | Jianrong Li Haixia Lu Junli Zhu | 2009 | Trends in Food Sci- ence & Technology2009，20，： | 1 |
| 12 | Effect of tea polyphenols on the physical and chemical characteristics of dried-seasoned squid (Dosidicus gigas) during storage显示文摘 | DONG Liangliang ZHU Junli LI Xuepeng | 2013 | Food Control2013，31，2： | 1 |
| 13 | Postmortem changes in yellow grouper (Epinephelus awoara) fillets stored under vacuum packaging at 0 ℃显示文摘 | XUEPENG LI JIANRONG LI JUNLI ZHU et al | 2011 | Food Chemistry2011，，126： | 1 |
| 14 | Key immunity characteristics of diverse stages of brucellosis in rural population from Inner Mongolia, China显示文摘Background: Brucellosis poses a serious threat to human and animal health,particularly in developing countries such as China.The Inner Mongolia Autonomous Region is one of the most severely brucellosis-endemic provinces in China.Currently,the host immune responses functioning to control Brucella infection and development remain poorly understood.The aim of this study is to further clarify the key immunity characteristics of diverse stages of brucellosis in Inner Mongolia.Methods: We collected a total of 733 blood samples from acute(n=137),chronic(n=316),inapparent(n=35),recovery(n=99),and healthy(n=146)groups from the rural community of Inner Mongolia between 2014 and 2015.The proportions of CD4^(+),CD8^(+),Th1,Th2,and Th17 T cells in peripheral blood and the expression of TLR2 and TLR4 in lymphocytes,monocytes and granulocytes were examined using flow cytometry analysis.The differences among the five groups were compared using one-way ANOVA and the Kruskal–Wallis method,respectively.Results: Our results revealed that the proportions of CD4^(+) and CD8^(+) T cells were significantly different among the acute,chronic,recovery,and healthy control groups(P<0.05),with lower proportions of CD4^(+) T cells and a higher proportion of CD8^(+) T cells in the acute,chronic,and recovery groups.The proportion of Th1 cells in the acute,chronic,and inapparent groups was higher than that in the healthy and recovery groups;however,there was no significant difference between patients and healthy individuals(P>0.05).The proportion of Th2 lymphocytes was significantly higher in the acute and healthy groups than in the inapparent group(P<0.05).The proportion of Th17 cells in the acute group was significantly higher than that in the healthy control,chronic,and inapparent groups(P<0.05).Finally,the highest expression of TLR4 in lymphocytes,monocytes and granulocytes was observed in the recovery group,and this was followed by the acute,chronic,healthy control,and inapparent groups.There was a significant difference between the recovery group and the other groups,except for the acute group(P<0.05).Moreover,a correlation in TLR4 expression was observed in lymphocytes,monocytes and granulocytes among the five groups(r>0.5),except for the inapparent group between lymphocytes and granulocytes(r=0.34).Conclusions: Two key factors(CD8^(+)T cells and TLR4)in human immune profiles may closely correlate with the progression of brucellosis.The detailed function of TLR4 in the context of a greater number of cell types or tissues in human or animal brucellosis and in larger samples should be further explored in the future. | Yongzhang Zhu Li Shi Yige Zeng Dongri Piao Yingbo Xie Juan Du Meng Gao Wei Gao Junli Tian Jun Yue Min Li XiaoKui Guo Yufeng Yao YaoXia Kang | 2022 | Infectious Diseases of Poverty2022，11，3： | 1 |
| 15 | Determination of formaldehyde in squid by high - performance liquid chromatography显示文摘 | Jianrong Li Junli Zhu Lifang Ye | | 0，，1： | 1 |
| 16 | Structural variation and parallel evolution of apomixis in citrus during domestication and diversification显示文摘Apomixis,or asexual seed formation,is prevalent in Citrinae via a mechanism termed nucellar or adventitious embryony.Here,multiple embryos of a maternal genotype form directly from nucellar cells in the ovule and can outcompete the developing zygotic embryo as they utilize the sexually derived endosperm for growth.Whilst nucellar embryony enables the propagation of clonal plants of maternal genetic constitution,it is also a barrier to effective breeding through hybridization.To address the genetics and evolution of apomixis in Citrinae,a chromosome-level genome of the Hongkong kumquat(Fortunella hindsii)was assembled following a genome-wide variation map including structural variants(SVs)based on 234 Citrinae accessions.This map revealed that hybrid citrus cultivars shelter genome-wide deleterious mutations and SVs into heterozygous states free from recessive selection,which may explain the capability of nucellar embryony in most cultivars during Citrinae diversification.Analyses revealed that parallel evolution may explain the repeated origin of apomixis in different genera of Citrinae.Within Fortunella,we found that apomixis of some varieties originated via introgression.In apomictic Fortunella,the locus associated with apomixis contains the FhRWP gene,encoding an RWP-RK domain-containing protein previously shown to be required for nucellar embryogenesis in Citrus.We found the heterozygous SV in the FhRWP and CitRWP promoters from apomictic Citrus and Fortunella,due to either two or three miniature inverted transposon element(MITE)insertions.A transcription factor,FhARID,encoding an AT-rich interaction domain-containing protein binds to the MITEs in the promoter of apomictic varieties,which facilitates induction of nucellar embryogenesis.This study provides evolutionary genomic and molecular insights into apomixis in Citrinae and has potential ramifications for citrus breeding. | Nan Wang Xietian Song Junli Ye Siqi Zhang Zhen Cao Chenqiao Zhu Jianbing Hu Yin Zhou Yue Huang Shuo Cao Zhongjie Liu Xiaomeng Wu Lijun Chai Wenwu Guo Qiang Xu Brandon S.Gaut Anna M.G.Koltunow Yongfeng Zhou Xiuxin Deng | 2022 | National Science Review2022，9，10： | 1 |
| 17 | Purification and Properties of γ-Glutamyltranspeptidase from Lentinula edodes显示文摘 | YIN Jie ZHU Junli FU Linglin LI Jianrong | 2009 | 食用菌学报2009，16，2： | 1 |
| 18 | An Entropy-Based Weighted Concept Lattice for Merging Multi-Source Geo-Ontologies显示文摘 | Junli Li Zongyi He Qiaoli Zhu | 2013 | Entropy2013，15，： | 1 |
| 19 | Effect of tea polyphenols on the physical and chemical characteristics of dried-seasoned squid ( Dosidicus gigas ) during storage显示文摘 | Liangliang Dong Junli Zhu Xuepeng Li Jianrong Li | 2012 | Food Control2012，，： | 1 |
| 20 | Coating effects of tea polyphenol and rosemary extract combined with chitosan on the storage quality of large yellow croaker ( Pseudosciaena crocea )显示文摘 | Tingting Li Wenzhong Hu Jianrong Li Xuguang Zhang Junli Zhu Xuepeng Li | 2011 | Food Control2011，，1： | 1 |
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