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| 1 | Production of milk - clotting enzyme by Bacillus subtilis B1 from wheat bran 显示文摘 | Zhongyang Ding Shuangping Liu Zhenghua Gu | 2011 | African Journal of Biotechnology2011，，93： | 1 |
| 2 | Probiotic properties of lactic acid bacteria isolated from stool samples of longevous people in regions of Hotan, Xinjiang and Bama, Guangxi, China显示文摘 | GU Ruixia YANG Zhenquan LI Zhenghua | 2008 | Anaerobe2008，14，6： | 1 |
| 3 | Solution-based synthesis of chalcostibite (CuSbS2) nanobricks for solar energy conversion显示文摘 | YAN Chang SU Zhenghua GU Ening | 2012 | RSC Advances2012，2，10： | 1 |
| 4 | Effects of organic acids on adsorption of lead onto montmorillonite,goethite and humic acid显示文摘 | WU Zhenghua GU Zhimang WANG Xiaorong | 2003 | Environmental Pollution2003，121，： | 1 |
| 5 | Supplementation with yak (Bos grunniens) bone collagen hydrolysate altered the structure of gut microbiota and elevated short-chain fatty acid production in mice显示文摘In this study, yak bone collagen hydrolysate(YBCH)was produced by mixed proteases and provided to standard-diet mice at a different dose(low dose(LD), medium dose(MD), and high dose(HD))to investigate its effects on the composition of gut microbiota and short-chain fatty acids(SCFA)production. It was found that YBCH was mainly composed of small molecular peptides whose molecular weight below 2 000 Da. Notably, supplementation with different doses of YBCH could significantly downregulate the ratio of Firmicutes to Bacteroidetes in the fecal microbiota. At the family level, the Lachnospiraceae abundance was significantly reduced in the YBCH gavage groups(mean reduction ratio 41.7 %, 35.2%, and 36.4% for LD, MD, and HD group, respectively). The predicted functions of gut microbes in the MD group were significantly increased at “lipid metabolism” and “glycan biosynthesis and metabolism”. Moreover, the SCFA production in the YBCH groups was elevated. Especially, the concentration of acetic acid, propionic acid, and butyric acid in the MD group was separately increased 79.7%, 89.2%, and 78.8% than that in the NC group. These results indicated that YBCH might be applied in the development of functional food for intestinal microecological regulation. | Zitao Guo Dalong Yi Bo Hu Lingyu Zhu Ji Zhang Yuliang Yang Chunyu Liu Yi Shi Zhenghua Gu Yu Xin Huaigao Liu Liang Zhang | 2023 | Food Science and Human Wellness2023，12，5： | 1 |
| 6 | Probiotic properties of lactic acid bacteria isolated from stool samples of longevous people in regions of Hotan, Xinjiang and Bama, Guangxi, China显示文摘 | GU Ruixia YANG Zhenquan LI Zhenghua | 2008 | Anaerobe2008，14，6： | 1 |
| 7 | Preliminary Study on the Waterlogging Tolerance of 116 Corn Materials显示文摘Maize is one of the most important food crops in the world.With the global warming,waterlogging stress has become an important abiotic stress factor that affects crop growth,including maize.Waterlogging seriously affects 10%of the arable land and can lead to a 15%-80%reduction in crop yield[1].In this study,115 inbred line materials commonly used in spring maize planting areas in the Jianghan Plain,Hubei,and maize inbred line B73 with complete genome information,were collected and stressed by waterlogging for two weeks in the seven-leaf and one-heart stage,and the survival rate was statistically compared and analyzed,aiming to screen germplasms with strong waterlogging tolerance for the genetic improvement of waterlogging tolerance of Hubei maize lines. | Shilong ZHANG Haitao JIA Yinshan GU Zhenghua HE Yiqin HUANG | 2020 | Agricultural Biotechnology2020，9，6： | 0 |
| 8 | The alteration of gut microbiota by bioactive peptides:a review显示文摘Evidences suggest that the homeostasis of gut microbiota is among the most important factors for maintaining the physical and mental health of the host.Among the multiple factors affecting the homeostasis of gut microbiota,diet is one of the decisive factors.Bioactive peptides derived from protein hydrolyzed by protease or fermented by microorganism have many physiological activities that their parent proteins do not have.Currently,bioactive peptides attract more and more attention due to their bidirectional interaction with gut microbes.It has been reported that some bioactive peptides could alter the composition of gut microbiota by influencing the intestinal microenvironment.Meanwhile,quite a few bioactive peptides that are released by gut microbes or intestinal cells could resist the pathogenic bacteria to sustain the homeostasis of gut microbiota.In this review,some exogenous bioactive peptides derived from food and some endogenous bioactive peptides released from intestinal cells or microbes were discussed to summary their effects on the modulation of gut microbiota.This review is expected to provide new ideas for related research,and as well to promote the application of bioactive peptides in the fields of food and medicine. | Zitao Guo Dalong Yi Bo Hu Yi Shi Yu Xin Zhenghua Gu Huaigao Liu Liang Zhang | 2021 | Systems Microbiology and Biomanufacturing2021，1，4： | 0 |
| 9 | CcpA mutants infuence selective carbon source utilization by changing interactions with target genes in Bacillus licheniformis显示文摘The gram-positive bacterium Bacillus licheniformis exhibits obvious selective utilization on carbon sources.This process is mainly governed by the global regulator catabolite control protein A(CcpA),which can recognize and bind to multiple target genes that are widely distributed in metabolic pathways.Although the DNA-binding domain of CcpA has been predicted,the infuence of key amino acids on target gene recognition and binding has yet to be uncovered.In this study,the impact of Lys31,Ile42 and Leu56 on in vitro protein–DNA interactions and in vivo carbon source selective utilization was investigated.The results showed that alanine substitution of Lys31 and Ile42,located within the 3rd helices of the DNAbinding domain,signifcantly weakened the binding strength between CcpA and target genes.These mutations also lead to alleviated repression of xylose utilization in the presence of glucose.On the other hand,the Leu56Arg mutant in the 4th helices exhibited enhanced binding afnity compared with that of the wild-type one.When this mutant was used to replace the native one in B.licheniformis cells,the selective utilization of glucose over xylose increased.This study provides a new strategy for understanding the relationship between the function and structure of regulatory proteins.This study also used a new strategy was used to regulate carbon source utilization beyond CCR engineering. | Yupeng Zhang Youran Li Fengxu Xiao Hanrong Wang Liang Zhang Zhongyang Ding Sha Xu Zhenghua Gu Guiyang Shi | 2022 | Systems Microbiology and Biomanufacturing2022，2，1： | 0 |
| 10 | Heterologous expression,refolding,and characterization of a calcium-independent phospholipase A1 from Streptomyces albidoflavus显示文摘Phospholipase A1(PLA_(1))is a member of the hydrolase family with applications in various fields,especially in the food industry.A calcium-independent PLA_(1) from Streptomyces albidoflavus was expressed in E.coli BL21(DE3)in this study.The results indicated that the soluble expression of PLA_(1) was at low level,which was possibly due to the toxicity of PLA_(1) to the host.In contrast,the expression of the enzyme as inclusion bodies exhibited a high-level expression and 0.3 mg inclusion bodies protein could be derived from 1 mL culture medium.Furthermore,the renaturation of PLA_(1) was achieved through a direct dilution method,yielding 29.6 U/mL PLA_(1) activity after 16 h of renaturation at 4℃.For improving the efficiency of the dilution refolding process,a continuous refolding strategy was established,and 155 U/mL PLA_(1) activity was derived from the continuous refolding process.With soybean lecithin as the substrate,the specific activity of purified renatured PLA_(1) was 1380 U/mg and the optimal temperature and pH was found to be 60℃ and 6.5.In addition,the renatured PLA_(1) was observed with better activity towards phosphatidyl inositol,whilst lipase activity was detected when the catalyzing temperature was below 55℃.Overall,this study provides a possible solution to obtain calcium-independent PLA_(1) with high yield by heterologous expression in E.coli and hence to promote its further application in the field of food industry. | Shi Cheng Zitao Guo Chaojuan Liang Yi Shi Peng Geng Yu Xin Zhenghua Gu Liang Zhang | 2022 | Systems Microbiology and Biomanufacturing2022，2，3： | 0 |
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