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| 1 | AFLP analysis revealed differences in genetic diversity of four natural populations of Manila clam (Ruditapes philippinarum) in China显示文摘The amplified fragment length polymorphism (AFLP) technology was used to analyze the genetic diversities in four natural populations of Manila clam (Ruditapes philippinarum), distributed in four sea areas of China, i.e., the Bohai Sea, the Huanghai Sea, the East China Sea and the South China Sea. Two hundred and sixty-four AFLP loci were analysed in 195 individuals and revealed high levels of genetic diversity. The percentage of polymorphic loci ranged from 92.13% to 96.06% and the Shannon’s information index was from 0.256 8 to 0.275 6. By analyzing molecular variance (AMOVA), it was found that there were high levels of genetic differentiation between populations of Qingdao and the other three sea areas. Cluster analysis by Nei’s pairwise distances grouped specimens by geographical origin, except the population of Qingdao. A conclusion can be drawn that there are high genetic diversities in the four natural populations of Manila clam in China and some distinct differences existed among and between the four populations. The results also indicated that human cultivation activities will have great influence on the genetic structure of the population of Qingdao. | LIU Xiangquan BAO Zhenmin HU Jingjie WANG Shi ZHAN Aibin LIU Hui FANG Jianguang WANG Rucai | 2007 | Acta Oceanologica Sinica2007，26，1： | 18 |
| 2 | Chromosomal localization of the major ribosomal RNA genes in scallop Chlamys farreri显示文摘The chromosomes of Chlamys farreri were analyzed by means of silver staining and fluorescence in situ hybridization (FISH) with 18S—28S rDNA probe. Probe was made by PCR amplification of a DNA fragment containing internal transcribed spacers ITS1 between 18S and 5.8S ribosomal RNA gene, ITS2 between 5.8S and 28S ribosomal RNA gene and 5.8S rRNA gene, and labeled by PCR incorporation of bio-16-dUTP. FISH signals were located on the short arm of subtelocentric chromosome 10. After silver-staining, nucleolus organizer regions (NORs) could be observed on the telomere of the short arm of chromosome 10. However, one metaphase spread displayed an additional silver spot on the short arm of subtelocentric chromosome 12. | HUANG Xiaoting BAO Zhenmin BI Ke HU Jingjie ZHANG Can ZHANG Quanqi HU Xiaoli | 2006 | Acta Oceanologica Sinica2006，25，3： | 10 |
| 3 | Existence of Microsatellites in Expressed Sequence Tags of Common Carp (Cyprinus carpio L.) Available in GenBank dbEST Database显示文摘表示顺序标注的普通鲤鱼(EST ) 为存在 ofmicrosatellites,或简单顺序重复(SSR ) 被分析。在 NCBI dbEST 数据库, 10612sequences 的一个总数在 2004 年 12 月 31 日前被登记。2-6 nucleotidemicrosatellites 的完全的搜索导致了 513 包含 SSR EST 的鉴定,为 4.8% 总数的财务。聚合分析显示包含 SSR EST 的 73 个序列掉进 27 个组并且留下 440 EST 是独立的。467 唯一的包含 SSR EST 的一个总数被识别。这些 EST-SSRs 包含了许多简单顺序类型,并且 di-nucleotiderepeats 和 tri-nucleotiderepeats 是最丰富的,为 42.1% 全部和 27.9% 的财务分别地。thedinucleotide 重复, CA/TG 是最丰富的,由 GA/TC 列在后面。BLASTx 搜索证明 SSR loci 的 that38.1% 能与基因或蛋白质被联系知道或包含 SSR EST 的未知 function.BLASTx 搜索也在 zebrafishsequences 上显示出点击的高频率(98/179 ) 。 | HU Jingjie WANG Xiaolong HU Xiaoli BAO Zhenmin | 2006 | Journal of Ocean University of China2006，5，1： | 8 |
| 4 | Assessing genetic diversity of wild populations of Japanese flounderusing AFLP markers显示文摘Amplified fragment length polymorphism (AFLP) analysis was used to evaluate the genetic diversity of four wild geographical populations of Japanese flounder (Paralichthys olivaceus). A total of 775 loci (58.32% of which was polymorphic) in the range between 100 and 1 300 base pairs were detected from 110 individuals using seven primer combinations. The percentage of polymorphic loci detected by single primer combination for each population was calculated, ranging from 19.59% to 53.33%. Genetic similarities within and among the populations were calculated from the binary matrices of presence-absence. Phylogenetic tree of four populations was constructed by using the UPGMA method using PHYLIP Version 3.5. According to intrapopulation genetic similarities, CW population displayed the highest genetic diversity value and KY population had the lowest genetic diversity value. The distance between CW and CF populations was the farthest, which was possibly resulted from the farthest distance of Weihai of Shandong and Fujian of China compared with the geographical distance between other locations of populations. The subpopulation differentiation value (G_ st ) is 0.356 5, showing a certain extent of differentiation among the four geographical populations. AFLP technology was confirmed to be an effective tool to assess within-and among-population genetic diversity of Japanese flounder. The present survey provided significant insights for research in the Japanese flounder breeding program. | XU Xiaofei ZHANG Quanqi WANG Zhigang QI Jie ZHANG Zhifeng BAO Zhenmin Heisuke Nakagawa | 2006 | Acta Oceanologica Sinica2006，25，3： | 7 |
| 5 | Circular RNA circStag1 promotes bone regeneration by interacting with HuR显示文摘Postmenopausal osteoporosis is a common bone metabolic disorder characterized by deterioration of the bone microarchitecture,leading to an increased risk of fractures.Recently,circular RNAs(circ RNAs)have been demonstrated to play pivotal roles in regulating bone metabolism.However,the underlying functions of circ RNAs in bone metabolism in postmenopausal osteoporosis remain obscure.Here,we report that circ Stag1 is a critical osteoporosis-related circ RNA that shows significantly downregulated expression in osteoporotic bone marrow mesenchymal stem cells(BMSCs)and clinical bone tissue samples from patients with osteoporosis.Overexpression of circ Stag1 significantly promoted the osteogenic capability of BMSCs.Mechanistically,we found that circ Stag1 interacts with human antigen R(Hu R),an RNA-binding protein,and promotes the translocation of Hu R into the cytoplasm.A high cytoplasmic level of Hu R led to the activation of the Wnt signaling pathway by stabilizing and enhancing low-density lipoprotein receptor-related protein 5/6(Lrp5/6)andβ-catenin expression,thereby stimulating the osteogenic differentiation of BMSCs.Furthermore,overexpression of circ Stag1 in vivo by circ Stag1-loaded adeno-associated virus(circ Stag1-AAV)promoted new bone formation,thereby preventing bone loss in ovariectomized rats.Collectively,we show that circ Stag1 plays a pivotal role in promoting the regeneration of bone tissue via Hu R/Wnt signaling,which may provide new strategies to prevent bone metabolic disorders such as postmenopausal osteoporosis. | Gaoyang Chen Canling Long Shang Wang Zhenmin Wang Xin Chen Wanze Tang Xiaoqin He Zhiteng Bao Baoyu Tan Jin Zhao Yongheng Xie Zhizhong Li Dazhi Yang Guozhi Xiao Songlin Peng | 2022 | Bone Research2022，10，3： | 7 |
| 6 | Study on purification and ultrastructure of a baculovirus in Penaeus chinensis显示文摘 | Shi Tuo, Kong Jie, Bao Zhenmin, Liu Ping and Wang Chongming (Huanghai Sea Fisheries Research Institute, Qingdao 266071, China)Liang Xingming, (College of Marine Life Sciences, Ocean University of Qingdao, Qingdao 266003, China) | 1998 | Acta Oceanologica Sinica1998，17，4： | 4 |
| 7 | Mapping Toll-Like Receptor Signaling Pathway Genes of Zhikong Scallop(Chlamys farreri) with FISH显示文摘Toll-like receptor(TLR) signaling pathway plays a pivotal role in the innate immune system. Studies on TLR signaling pathway genes in Zhikong scallop(Chlamys farreri) have mainly focused on sequence analysis and expression profiling, no research has been carried out on their localization. The chromosomal position of TLR signaling pathway genes can be valuable for assemblying scallop genome and analysizing gene regulatory networks. In the present study, five key TLR signaling pathway genes(Cf TLR, Cf Myd88, Cf TRAF6, Cf NFκB, and Cf IκB) containing bacterial artificial chromosomes(BACs) were isolated and physically mapped through fluorescence in situ hybridization on five non-homologous chromosome pairs, showing a similar distribution to another five model species. The isolation and mapping of these key immune genes of C. farreri will aid to the research on innate immunity, assignment of interested genes to chromosomes, and integration of physical, linkage and cytogenetic maps of this species. | ZHAO Bosong ZHAO Liang LIAO Huan CHENG Jie LIAN Shanshan LI Xuan HUANG Xiaoting BAO Zhenmin | 2015 | Journal of Ocean University of China2015，14，6： | 3 |
| 8 | Effect of Epinephrine on the Settlement and Metamorphosis of Manila Clam Larvae显示文摘化学动机和 DDRT-PCR (微分显示颠倒 transcriptionPCR ) 被采用在马尼拉蚌幼虫的解决和变形上调查肾上腺素(EPI ) 的效果。化学动机证明 EPI 根本在马尼拉蚌幼虫的变形上有效果到某程度集中并且在设计的所有处理。动机的最重要的结果在 10 ~ 的集中被获得(-6) 摩尔 L^(-1) 并且为 4h,用六教材对的 .DDRT-PCR 证明基因表达式模式是相当不同的 betweenEPI 处理和控制。343 个扩大乐队总共被获得,在哪个之中,(19.53%) 67 是差别出现。因此, EPI 在眼睛点的基因表示上有效果幼虫的马尼拉蚌。EPI 是为马尼拉蚌的解决和变形 inducer,这能被假设。EPI 可以在膜上由绑定导致幼虫解决和变形到受体然后改变幼虫房间的基因表示。 | LU Sumin BAO Zhenmin LIU Hui FANG Jianguang | 2006 | Journal of Ocean University of China2006，5，2： | 3 |
| 9 | Fine‑mapping and association analysis of candidate genes for papilla number in sea cucumber,Apostichopus japonicus显示文摘The papilla number is one of the most economically important traits of sea cucumber in the China marketing trade.However,the genetic basis for papilla number diversity in holothurians is still scarce.In the present study,we conducted genomewide association studies(GWAS)for the trait papilla number of sea cucumbers utilizing a set of 400,186 high-quality SNPs derived from 200 sea cucumbers.Two significant trait-associated SNPs that passed Bonferroni correction(P<1.25E−7)were located in the intergenic region near PATS1 and the genic region of EIF4G,which were reported to play a pivotal role in cell growth and proliferation.The fine-mapping regions around the top two lead SNPs provided precise causative loci/genes related to papilla formation and cellular activity,including PPP2R3C,GBP1,and BCAS3.Potential SNPs with P<1E−4 were acquired for the following GO and KEGG enrichment analysis.Moreover,the two lead SNPs were verified in another population of sea cucumber,and the expressive detection of three potential candidate genes PATS1,PPP2R3C,and EIF4G that near or cover the two lead SNPs was conducted in papilla tissue of TG(Top papilla number group)and BG(Bottom papilla number group)by qRT-PCR.We found the significantly higher expression profile of PATS1(3.34-fold),PPP2R3C(4.90-fold),and EIF4G(4.23-fold)in TG,implying their potential function in papilla polymorphism.The present results provide valuable information to decipher the phenotype differences of the papilla trait and will provide a scientific basis for selective breeding in sea cucumbers. | Xinghai Zhu Ping Ni Marc Sturrock Yangfan Wang Jun Ding Yaqing Chang Jingjie Hu Zhenmin Bao | 2022 | Marine Life Science & Technology2022，4，3： | 2 |
| 10 | Development of 101 Novel EST-Derived Single Nucleotide Polymorphism Markers for Zhikong Scallop (Chlamys farreri)显示文摘Zhikong scallop(Chlamys farreri) is an important maricultured species in China.Many researches on this species,such as population genetics and QTL fine-mapping,need a large number of molecular markers.In this study,based on the expressed sequence tags(EST),a total of 300 putative single nucleotide polymorphisms(SNPs) were selected and validated using high resolution melting(HRM) technology with unlabeled probe.Of them,101(33.7%) were found to be polymorphic in 48 individuals from 4 populations.Further evaluation with 48 individuals from Qingdao population showed that all the polymorphic loci had two alleles with the minor allele frequency ranged from 0.046 to 0.500.The observed and expected heterozygosities ranged from 0.000 to 0.925 and from 0.089 to 0.505,respectively.Fifteen loci deviated significantly from Hardy-Weinberg equilibrium and significant linkage disequilibrate was detected in one pair of markers.BLASTx gave significant hits for 72 of the 101 polymorphic SNP-containing ESTs.Thirty four polymorphic SNP loci were predicted to be non-synonymous substitutions as they caused either the change of codons(33 SNPs) or pretermination of translation(1 SNP).The markers developed can be used for the population studies and genetic improvement on Zhikong scallop. | LI Jiqin BAO Zhenmin LI Ling WANG Xiaojian WANG Shi HU Xiaoli | 2013 | Journal of Ocean University of China2013，12，3： | 2 |
| 11 | Amplicon-Based Illumina Sequencing and Quantitative PCR Reveals Nanoplankton Diversity and Biomass in Surface Water of Qinhuangdao Coastal Area, China显示文摘Aureococcus anophagefferens caused brown tides for three consecutive years from 2009 to 2011 in the coastal waters of Qinhuangdao, China, with numerous, widespread ecological and economic impact on ecosystems. To understand the population dy- namics of nanoplankton during the brown tides, sequences of the V9 region of the 18S rDNA gene, used as a marker, were analyzed by Illumina sequencing to assess nanoplankton biomass, and real-time fluorescence quantitative PCR was performed to analyze spa- tial variation in the 18S rDNA copy concentrations of nanoplankton off the Qinhuangdao coast in July, 2011. The results showed that A. anophagefferens and Minutocellus polymorphus were the dominant species in the local phytoplankton community during the brown tide in July 2011. The highest 18S rDNA copy concentrations of A. anophagefferens and M. polymorphus were detected at stations SHG and FN, respectively. The central area most strongly affected by the brown tide migrated southward from 2011 to 2013. Redundancy analysis (RDA) showed that the decreasing NOx concentration might provide suitable nutrient conditions for the A. anophagefferens outbreak. During the brown tide caused by A. anophagefferens, other phytoplankton, such as diatoms, cryptophytes, chlorophytes, dinoflagellates and other flagellates, could co-occur with it. For zooplankton, due to less selective feeding behavior, Amoebozoa was the most abundant zooplankton at station SHG, while Ciliophora was the most abundant zooplankton at other sta- tions for its more selective feeding. | QIAO Ling YU Jie LI Ying GUO Haobing ZHEN Yu ZHANG Lingling MI Tiezhu BAO Zhenmin XU Xiaochun | 2019 | Journal of Ocean University of China2019，18，4： | 2 |
| 12 | Glutathione S-transferase genes in scallops and their diverse expression patterns after exposure to PST-producing dinoflagellates显示文摘The glutathione S-transferases(GSTs)are a superfamily of enzymes that function in cellular protection against toxic substances and oxidative stress.Bivalves could accumulate high concentration of paralytic shellfish toxins(PSTs)from harmful algae.To understand the possible involvement of GSTs in protecting bivalves during PST accumulation and metabolism,the GST genes were systemically analyzed in two cultured scallops,Azumapecten farreri and Mizuhopecten yessoensis,which were reported for PST deposition during harmful algae bloom.A total of 35 and 37 GSTs were identified in A.farreri(AfGSTs)and M.yessoensis(MyGSTs)genome,respectively,and the expansion of the sigma class from the cytosolic subfamily was observed.In both scallop species,sigma class GSTs showed higher expression than other members.The high GSTs expression was detected mainly during/after larvae stages and in the two most toxic organs,hepatopancreas and kidney.After ingesting PST-producing dinoflagellates,all the regulated AfGSTs in the hepatopancrcas were from the sigma class,but with opposite regulation pattern between Alexandrium catenella and A.minutum exposure.In scallop kidneys,where PSTs transformed into higher toxicity,more AfGSTs were regulated than in the hepatopancreas,and most of them were from the sigma class,with similar regulation pattern between A.catenella and A.minutum exposure.In M.yessoensis exposed to A.catenella,MyGST-σ2 was the only up-regulated MyGST in both hepatopancreas and kidney.Our results suggested the possible diverse function of scallop GSTs and the importance of sigma class in the defense against PSTs,which would contribute to the adaptive evolution of scallops in marine environments. | Jiarun Lou Jie Cheng Xiaogang Xun Xu Li Moli Li Xiangchao Zhang Tingting Li Zhenmin Bao Xiaoli Hu | 2020 | Marine Life Science & Technology2020，2，3： | 2 |
| 13 | An Efficient Procedure for Isolating Microsatellite DNAs from Sea Cucumber(Apostichopus japonicus)显示文摘The construction of enrichment library proves to be one of the efficient approaches for isolating microsatellites in this study. The genomic DNA of sea cucumber was digested with HaeIII and size-selected DNA fragments (250-700 bp) were ligated to an adaptor. Microsatellite-containing sequences were captured by using a combination of GA and CA probes, which were attached to a nylon membrane. The microsatellite enrichment library constructed in this study consisted of approximately 700 clones. Two hun-dred and thirty-two clones reacted positively after the library screening procedure. Of the 50 clones sequenced, all contained at least one microsatellite and one duplicate clone was found. Approximately 86% of the sequenced fragments permitted to design primers for sequence tagged microsatellite site (STMS). | HU Jingjie, ZHAN Aibin, LU Wei, HU Xiaoli, and BAO Zhenmin* Laboratory of Marine Genetics and Breeding (MGB), Division of Life Science and Biotechnology, Ocean University of China, Qingdao 266003, P. R. China | 2007 | Journal of Ocean University of China2007，6，1： | 2 |
| 14 | Genetic diversity and specific markers in four scallop species, Patinopecten yessoensis, Argopecten irradians, Chlamys nobilis and C.farreri显示文摘The AFLP (amplified fragment length polymorphism)technique was used to analyse the genetic diversity in four scallop species,Patinopecten yessoensis, Argopecten irradians, Chlamys nobilis and C.farreri. The genetic similarity indexes of these four species are0.841 5, 0.786 3, 0.719 0 and 0.673 1, while Shannon diversity indexes are 43.52, 58.87, 80.16 and 92.83, respectively. As analyzed,the genetic diversities in two native species, i.e., C.farreri and C. nobilis, are higher than those in other two introduced species, A.irradians and P. yessoensis. The results also showed that C. nobilis and C. farreri shared the most common loci. The genetic distanceindicated that C. nobilis and C. farreri are closely related. Moreover, out of 510 AFLP markers, 21 specific bands are found todistinguish the four species scallops and these markers may be applied to the specific germplasm characterization and molecularassistant classification in scallops. | CHEN Shengping BAO Zhenmin PAN Jie HU Jingjie | 2005 | Acta Oceanologica Sinica2005，24，4： | 2 |
| 15 | Solvothermal-assisted assembly of MoS2 nanocages on graphene sheets to enhance the electrochemical performance of lithium-ion battery显示文摘An ordered hollow M0S2 nanocages/RGO nanocomposite is constructed by a simple solvothermal-assisted assembly method combined with freeze-drying and annealing.In this novel nanostructure,hollow M0S2 nanocages are homogeneously distributed on graphene sheets with a tight bond of C-O-Mo.The nanosized and hollow MoS2 nanocages can effectively accommodate the huge volume change during charge/discharge process and increase the number of electrochemical reaction active sites,accelerating the kinetics of lithiation/delithiation.The tight C-O-Mo bond between graphene and MoS2 further reinforces the structural stability,thus improve the electrical conductivity and substantially enhance the lithium storage performance of M0S2 anode material.As a result,this novel nanocomposite shows a long-cycle stability of 717.4 mAh·g^-1 after 800 cycles at a high current density of 3 A·g^-1,exhibiting great potential as an anode nanocomposite for advanced lithium-ion batteries. | Dafang He Yi Yang Zhenmin Liu Jin Shao Jian Wu Shun Wang Liming Shen Ningzhong Bao | 2020 | Nano Research2020，13，4： | 2 |
| 16 | Development and Characterization of Microsatellite Markers for the Pacific Abalone (Haliotis discus) via EST Database Mining显示文摘The EST database of the Pacific abalone (Haliotis discus) was mined for developing microsatellite markers. A total of 1476 EST sequences were registered in GenBank when data mining was performed. Fifty sequences (approximately 3.4%) were found to contain one or more microsatellites. Based on the length and GC content of the flanking regions, cluster analysis and BLASTN, 13 microsatellite-containing ESTs were selected for PCR primer design. The results showed that 10 out of 13 primer pairs could amplify scorable PCR products and showed polymorphism. The number of alleles ranged from 2 to 13 and the values of Ho and He varied from 0.1222 to 0.8611 and 0.2449 to 0.9311, respectively. No significant linkage disequilibrium (LD) between any pairs of these loci was found, and 6 of 10 loci conformed to the Hardy-Weinberg equilibrium (HWE). These EST-SSRs are therefore potential tools for studies of intraspecies variation and hybrid identification. | ZHAN Aibin BAO Zhenmin WANG Mingling CHANG Dan YUAN Jian WANG Xiaolong HU Xiaoli LIANG Chengzhu HU Jingjie | 2008 | Journal of Ocean University of China2008，7，2： | 2 |
| 17 | Comparative Studies on the Molecular Genetic Diversities among Haliotis discus hannai, H. discus discus and Their Hybrids显示文摘The hybrid (H. discus hannai♀× H. discus discus♂) shows strong heterosis in both growth and survival rates during aquaculture. In order to better understand the genetic basis of heterosis, AFLP markers are adopted to compare the genetic diversities of the two parents and their hybrids. Six primer combinations reveal 552 loci, among which 88 loci show significant difference between the two parent populations (P<0.01). Analysis of molecular variance (AMOVA) indicates the genetic variance between them is significantly different (P<0.001). Thus there should be genetic basis of heterosis for their hybrids. In contrast to parents, more loci with lower frequency are amplified in hybrids than those in parents, whereas the loci with 0% and 100% frequency are less in hybrids than those in parents. Moreover, the genetic diversities of hybrids increase since the similarity indexes are lower and heterozygosities are higher in hybrids than those in parents. In addition, the genetic distances between reciprocal F1s and H. discus discus are both smaller than those between reciprocal F1s H. discus hannai. | 万俊芬 BAO Zhenmin Zhang Quanqi Wang Xiaolong | 2004 | High Technology Letters2004，10，3： | 2 |
| 18 | Cytogenetic analysis in two scallops (Bivalvia:Pectinidae) by PRINS and PI banding显示文摘Cytogenetic analysis was performed for the bay scallop(Argopecten irradians Lamarck 1819) and the Japanese scallop(Patinopecten yessoensis Jay 1857) by primed in situ labeling(PRINS) and propidium iodide(PI) banding techniques.The PRINS analysis revealed that major rRNA genes were clustered in two loci on the telomeric regions of the short arms on two acrocentric chromosome pairs in A.irradians and on two submetacentric pairs in P.yessoensis.The histone H3 gene sites differed in number and location between these two species.The C-band-like patterns revealed by PI staining varied considerably between these two species.A.irradians displayed terminal bands at long arms on all chromosomes,centromeric bands on some pairs and interstitial bands on five pairs.P.yessoensis exhibited only centromeric bands on all chromosomes.These results would contribute to the better understanding of karyotype evolution in A.irradians and P.yessoensis. | ZHANG Lingling BAO Zhenmin WANG Jue WANG Shi HUANG Xiaoting HU Xiaoli HU Jingjie | 2007 | Acta Oceanologica Sinica2007，26，5： | 2 |
| 19 | A Population Genetic Analysis of Continuously Selected Chlamys farreri Populations显示文摘This study applied an optimized one-step 2b-RAD library construction strategy and performed simplified genome sequencing of 539 individuals from three continuously selected Chlamys farreri populations. SNP screening was performed using RAD typing software and population genetic parameters for the continuously selected populations from three generations(G1, G2, G3) were determined. The results showed that the optimized one-step 2b-RAD library construction strategy greatly simplified the experimental process, making it suitable for efficiently constructing a large number of libraries. A total of 18450 SNP markers were identified, which evenly distributed throughout the genome. Population genetic analysis of these three generations showed that the mean value of observed heterozygosity was 0.275 ± 0.177, 0.272 ± 0.181 and 0.275 ± 0.166, respectively. Meanwhile, the mean value of expected heterozygosity was 0.275 ± 0.141, 0.274 ± 0.145 and 0.280 ± 0.133, respectively. The Wright's fixation index(F) was 0.04291, 0.04976 and 0.06685, respectively. Markers deviated from Hardy–Weinberg equilibrium accounted for 10.34%, 12.64%, and 23.11%, and the Shannon diversity index was 0.0999 ± 0.0404, 0.0921 ± 0.0388 and 0.0733 ± 0.0308. FIS(also known as the inbreeding coefficient) of the three populations was 0.0256, 0.0323 and 0.0468, respectively. We suggested that the 2b-RAD method is well suited to population genetic studies of aquacultured organisms. Moreover, our results indicated that the continuous selection affected the population genetic structure of the cultured Penglai-Red scallop, but the change was not significant; therefore, population selection should continue. | ZHANG Lu LI Yuli LI Yangping YANG Zhihui LI Yuqiang WANG Yangfan WANG Shi BAO Zhenmin | 2018 | Journal of Ocean University of China2018，17，4： | 2 |
| 20 | Preliminary Analysis of Population Genetic Diversity of Cultivated Laminaria japonica Sporophyte via AFLP Technique显示文摘The amplified fragment length polymorphic DNA (AFLP) technique was adopted to estimate the population genetic polymorphism among 30 sporophytes of Laminaria japonica collected from a cultivating farm in Rongcheng,China.Three methods were used for genomic DNA extraction from Laminaria japonica sporophyte and only the products obtained using the improved genomic DNA extraction kit method proved qualified for AFLP analysis.The parameters of the method were optimized.Samples of forty milligrams and the cell lysis time of 120 min were suggested to replace the parameters recommended by the manufacturer.Thirty individuals of Laminaria japonica from the same cultivating site were investigated using one pair of selective primers.A total of 21 loci were obtained and 17 of them were polymorphic.The mean percent age of polymorphic loci of this population was 80.95%.The Nei's gene diversity (H) within this population was 0.3028 and the average Shannon's Information index (I) was 0.4498.A genetic distance matrix among different individuals was constructed as well.Through this study,an applicable AFLP genetic analysis working system for Laminaria japonica sporophyte was established.The results of this research also revealed a high level of genetic diversity within the studied population. | YI Heng SUI Zhenghong BAO Zhenmin | 2010 | Journal of Ocean University of China2010，9，1： | 2 |
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