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| 1 | A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants显示文摘CRISPR/Cas9 染色体指向系统被用于许多种类。然而,为植物报导的 mostCRISPR/Cas9 系统能仅仅修改一个或一些指向地点。这里,我们报导一个柔韧的 CRISPR/Cas9 向量系统,利用植物 codon 优化了 Cas9 基因,为方便、高效率的复合染色体在 monocot 和 dicot 植物编辑。我们设计了基于 PCR 的过程很快产生多重 sgRNA 表达式盒子,它能在一个轮由金门结扎或吉布森汇编克隆被装配进二进制 CRISPR/Cas9 向量。与这个系统,我们与 85.4% 变化评估的一般水准在米饭编辑了 46 个目标地点,主要在 biallelic 和同型结合的地位。我们推想在米饭的大约 16% 同型结合的变化通过相应基于再结合的修理跟随的非相应的加入结束的机制被产生。我们也获得了一致 biallelic,在 Arabidopsis T 1 植物的异质接合、同型结合、妄想的变化。在米饭和 Arabidopsis 的指向的变化是可继承的。我们在 T 0 米饭和 T 1 由同时的指向的 Arabidopsis 植物多重(多达八) 一个基因家庭,在一条 biosynthetic 小径的多重基因,或在单个基因的多重地点的成员。这个系统为为基本研究和基因改进在植物学习多重基因和基因家庭的功能提供了一个万用的工具箱。 | Xingliang Ma Qunyu Zhang Qinlong Zhu Wei Liu Yan Chen Rong Qiu Bin Wang Zhongfang Yang Heying Li Yuru Lin Yongyao Xie Rongxin Shen Shuifu Chen Zhi Wang Yuanling Chen Jingxin Guo Letian Chen Xiucai Zhao Zhicheng Dong Yao-Guang Liu | 2015 | Molecular Plant2015，8，8： | 272 |
| 2 | Temporal variation of soil carbon stock and its controlling factors over the last two decades on the southern Song-nen Plain,Heilongjiang Province显示文摘对全球气候变化的当前的背景,在土壤碳水池和它的控制因素的变化的学习可以在温室气体的缓解或改进帮助土壤角色的评估。这份报纸在 Heilongjiang 省在南部的 Song-nen 平原在土壤碳水池和他们的控制因素学习空间、时间的变化,用在二个不同时期由上收集的土壤数据在 20052007 的多种用途的地区性的 Geochemical 调查,和另外一个玷污在 19821990 进行的调查。学习区域是 1479 t/km2 并且在过去的 20 年里的碳来源,从直到 2005 的 1980 年代,从土壤的实际的碳排放是 0.12 Gt。温度,被发现了线性地被相关玷污器官的碳,是控制器官的碳满足的土壤的主导的 climatologic 因素。我们的学习在器官的碳由升起的温度引起了的土壤的潜在的损失是的相关区域和时间时期显示出那 0.10 Gt ,源于陆地使用变化的潜在的土壤碳排放是 0.09 Gt ,并且温暖和陆地使用变化引起的土壤碳( 0.19 Gt )的联合潜在的损失比得上石块燃料燃烧( 0.21 Gt )的。由于在土壤碳水池变化的时间延期,仍然在温暖和以后将逐渐地被释放的陆地使用变化引起的潜在的排放有 0.07 Gt。 | Xueqi Xia Zhongfang Yang Yan Liao Yujun Cui Yansheng Li | 2010 | Geoscience Frontiers2010，1，1： | 19 |
| 3 | μ-and m-calpain expression and activity changes following diethylstilbestrol injection in the rat anterior pituitary显示文摘Little is known about changes in calpain activity in the pituitary gland.In the present study,μ-and m-calpain activity changes were detected in the rat anterior pituitary following intraperitoneal injection of diethylstilbestrol.Double-immunofluorescence labeling confirmed colocalization of μ-and m-calpain in prolactin-secreting cells(lactotrophs).Western blot analysis revealed significantly increased expression of both calpains,which accompanied upregulated cytosol and membrane zymographic activities at 12 weeks following diethylstilbestrol injection,compared with rats injected with sunflower oil.Moreover,following estrogen injection,pituitary gland pathological damage gradually worsened with increasing time.Results demonstrated that estrogen regulated calpain expression and activity,and both calpains participated in the pathophysiological processes of the pituitary gland.Ubiquitous calpain expression could serve as an effective target for anti-estrogen drugs. | Weijiang Zhao Zhongfang Shi Fang Yuan Guilin Li Yazhuo Zhang Zhongcheng Wang | 2011 | Neural Regeneration Research2011，6，1： | 7 |
| 4 | Fucoxanthin induces growth arrest and apoptosis in human bladder cancer T24 cells by up-regulation of p21 and down-regulation of mortalin显示文摘Fucoxanthin,自然类胡萝卜素,被报导了在人的结肠癌房间,人的前列腺癌症房间,人的白血病房间,和人的上皮的颈的癌症房间举办反癌症活动。这研究被承担把 fucoxanthin 的分子的机制与人的膀胱癌症 T24 房间线作比较。 MTT 分析结果证明 5 和 10 M fucoxanthin 在生长拘捕在 G 0/G1伴随的一个剂量依赖者和时间依赖者举止禁止了 T24 房间的增长房间周期的阶段,被 p21 的起来规定调停,cyclin依赖的 kinase ( CDK )禁止的蛋白质和 CDK-2 , CDK-4 , cyclin D1 ,和 cyclin E 的下面规定。另外, 20 和 40 M fucoxanthin 由 mortalinp53 建筑群的废除和原子变异类型的 p53 的复活导致了 T24 房间的 apoptosis,它也让肿瘤 suppressor 作为野类型的 p53 工作。所有这些结果证明 T24 房间上的 fucoxanthin 的反癌症活动被 p21 的起来规定经由在 mortalin ,它是的表示水平,一个压力管理者和热的一个成员震惊的减少在低剂量和 apoptosis 在 G 0/G1阶段与房间周期拘捕联系蛋白质 70 ,在高剂量由劈开的 caspase-3 的起来规定列在后面。 | Linbo Wang Yang Zeng Ye Liu Xuansheng Hu Shuhong Li Yuepeng Wang Ling Li Zhongfang Lei Zhenya Zhang | 2014 | Acta Biochimica et Biophysica Sinica2014，46，10： | 5 |
| 5 | Safety and superior immunogenicity of heterologous boosting with an RBD-based SARS-CoV-2 mRNA vaccine in Chinese adults显示文摘Dear Editor,To May 2022,the COVID-19 pandemic has claimed more than 6.28 million lives,with more than 524 million confirmed cases worldwide.The recent emergence of highly transmissible Omicron variant of severe acute respiratory syndrome coronavirus 2(SARSCoV-2)has triggered another major surge in both confirmed cases and deaths.1 Ten COVID-19 vaccines have been approved by the World Health Organization(WHO)for emergency use,including the two mRNA vaccines,BNT162b2 and mRNA-1273,and two Chinese inactivated vaccines,CoronaVac and BBIBP-CorV.However,the rapid waning of vaccine-induced virus-neutralizing antibody titers and the continuous emergence of variants of concern(VOCs),including Alpha,Beta,Delta and Omicron,have created unprecedented challenges in the eradication of COVID-19 pandemic.2–4. | Xiaoqiang Liu Yuhua Li Zhongfang Wang Shouchun Cao Weijin Huang Lin Yuan Yi-Jiao Huang Yan Zheng Jingjing Chen Bo Ying Zuoyun Xiang Jin Shi Jincun Zhao Zhen Huang Cheng-Feng Qin | 2022 | Cell Research2022，32，8： | 5 |
| 6 | A study of soil organic carbon distribution and storage in the Northeast Plain of China显示文摘采用联合起来的土壤碳数量(USCA ) 途径,土壤碳存储基于在 20042006 进行的多种用途的地区性的 Geochemical 调查(MRGS ) 越过中国的东北平原被计算。结果显示在表层土(00.2 m ) 的器官的碳(SOC ) 存储,心土(01 m ) 和深土壤(01.8 m ) 是的土壤有 3327.8 t/km2, 12,904.7 t/km2 和 16,157.5 t/km2 的密度的 768.1 山, 2978.4 山和 3729.2 山分别地。而土壤碳密度从东北平原(辽宁)的南部的区域显示出一个清楚的增加趋势,这些价值以表层土特别地与国家一般水准一致,到中间(吉林)和北平原( Heilongjiang )碳密度,从 2284.2 增加了,到 3436.7 和 3861.5 t/km2 ,分别地。与在 19841986 从第二国家土壤调查获得的碳数据(SNSS ) 相比,从 MRGS 的表层土 SOC 存储值被发现由 320.59 山(29.4%) 减少了,与在 20 年的 16.0 山(l.73%) 的平均年度衰落。在平原的南部、中间、北的区域,分别地,土壤碳密度为整个平原与 1389.0 t/km2 的平均价值由 1060.6 t/km2, 1646.4 t/km2 和 1300.2 t/km2 减少了。这些调查结果显示在土壤碳密度的减少根据不同生态系统和陆地使用类型变化了。因此,土壤碳密度的比率被计算以便学习碳,并且进一步探索分发特征在生态系统之间的动态平衡,以及 SOC 的隐遁潜力。 | Xiaohuan Xi Zhongfang Yang Yujun Cui Shumei Sun Chengguang Yu Min Li | 2011 | Geoscience Frontiers2011，2，2： | 5 |
| 7 | N-heterocyclic carbene as a promising metal-free electrocatalyst with high efficiency for nitrogen reduction to ammonia显示文摘Electrocatalytic nitrogen reduction reaction(NRR)at ambient conditions holds great promise for sustainably synthesizing ammonia(NH3),while developing highly-efficient,long-term stable,and inexpensive catalysts to activate the inert N≡N bond is a key scientific issue.In this work,on the basis of the concept'N-heterocyclic carbenes(NHCs)',we propose a carbon decorated graphitic-carbon nitride(C/g-C3N4)as novel metal-free NRR electrocatalyst by means of density functional theory(DFT)computations.Our results reveal that the introduced C atom in g-C3N4 surface can be regarded as NHCs and catalytic sites for activating N≡N bond,and are stabilized by the g-C3N4 substrate due to sterically disfavored dimerization.Especially,this NHCs-based heterogeneous catalysis can efficiently reduce the activated N2 molecule to NH3 with a low overpotential of 0.05 V via an enzymatic mechanism.Our work is the first report of NHCs-based electrocatalyst for N2 fixation,thus opening an alternative avenue for advancing sustainable NH3 production. | Hongyan Li Le Yang Zhongxu Wang Peng Jin Jingxiang Zhao Zhongfang Chen | 2020 | Journal of Energy Chemistry2020，29，7： | 3 |
| 8 | Special characteristics of fluorescence and resonance Rayleigh scattering for cadmium telluride nanocrystal aqueous solution and its interactions with aminoglycoside antibiotics显示文摘CdTe nanocrystals(CdTe NCs) were achieved by reaction of CdCl2 with KHTe solution and were capped with sodium mercaptoacetate.The product was detected by transmission electron microscopy(TEM),high-resolution transmission electron microscopy(HRTEM),energy dispersive spectroscopy(EDS),fluorescence spectra,ultraviolet-visible spectra and X-ray diffraction(XRD).The CdTe NCs are of cubic structure and the average size is about 5 nm.The fluorescence quantum yield of CdTe NCs aqueous solution increased from 37% to 97% after 20 d under room light.The maximum λem of fluorescence changed from 543 nm to 510 nm and the blue shift was 33 nm.CdTe NCs aqueous solution can be steady for at least 10 months at 4℃ in a refrigerator.The resonance Rayleigh scattering(RRS) of CdTe NCs in the aqueous solution was investigated.The maximum scattering peak was located at about 554 nm.The interactions of CdTe NCs with amikacin sulfate(AS) and micronomicin sulfate(MS) were investigated respectively.The effects of AS and MS on fluorescence and RRS of CdTe NCs were analyzed.It was found that AS and MS quenched the photoluminescence of CdTe NCs and enhanced RRS of CdTe NCs.Under optimum conditions,there are linear relationships between quenching intensity(F0-F),intensity of RRS(I-I0) and concentration of AS and MS.The detection limits(3б) of AS and MS are respectively 3.4 ng·mL-1 and 2.6 ng·mL-1 by the fluorescence quenching method,and 15.2 ng·mL-1 and 14.0 ng·mL-1 by the RRS method.The methods have high sensitivity,thus CdTe NCs may be used as fluorescence probes and RRS probes for the detection of aminoglycoside antibiotics. | LI TaiShan LIU ShaoPu LIU ZhongFang HU XiaoLi ZHANG LiPing | 2009 | Science China Chemistry2009，52，1： | 2 |
| 9 | The Changes in Guilin Paddy Soil Organic Matter and Rice Yield under Long-Term Fertilization显示文摘Rational fertilization is an important measure to increase crop yield and soil fertility. Through analysis,this paper aims to master the change characteristics of soil organic matter and rice yield under different fertilizer treatments,in order to provide an important reference for the sustainable use of soil and effective fertilization. Long-term( 19 years) rice crop rotation experiments in waterloggogenic paddy soil were conducted to investigate the change trend of crop grain yield and soil organic matter with time,reveal the dynamic characteristics and relationship between main fertility factors and crop yields using comparative analysis at three sites with conventional fertilization and non-fertilization in Guilin. The results showed that compared with previous years,the rice yield increased by 53% under the fertilization treatment and degreased by 66% under the control. Over the years,the average soil organic matter( SOM) content under fertilization treatment was 23% higher than under CK treatment. This indicates that chemical fertilizer and organic manure application can increase the rice yield and soil organic matter,and high rice yield can be attributed to the SOM increase. | Qiuliang YU Zheng TANG Zhongfang LI Chunlan CHEN Xiaoxian TANG Yanli LIN | 2016 | Asian Agricultural Research2016，8，3： | 2 |
| 10 | Isolation and Preliminary Identification of Broad-spectrum Bacteriocin-producing Lactobacillus Strain CW3显示文摘[Objective]This study aimed to screen and preliminarily identify Lactobacillus strain CW3. [Method]Oxford cup double-plate method was used for preliminary screening of broad-spectrum bacteriocin-producing Lactobacillus strain; after eliminating the interference of organic acid and hydrogen peroxide,antibacterial substances containing protein properties produced by the screened strain were detected; finally,the obtained strain was identified. [Result]After elimination of interference factors such as organic acid and hydrogen peroxide,the fermentation supernatant of strain CW3 could still inhibit the growth of indicator strains; the antibacterial activity of fermentation broth was significantly reduced after treatment with trypsase and pepsase,which indicated that the produced antibacterial substances were bacteriocins. Results of physiological and biochemical identification showed that strain CW3 belonged to Lactobacillus plantarum. [Conclusion]CW3is a broad-spectrum bacteriocin-producing Lactobacillus plantarum strain. | Haoxin LV Weidong WANG Zhongfang TAN Feifei YANG Yingchun JIAO Yanping WANG Zongwei LI | 2014 | Agricultural Biotechnology2014，3，1： | 2 |
| 11 | Effects of Coupling Treatment of Acid and Aluminum under Soilless Culture on Water Content of Ginkgo biloba L.显示文摘In this experiment,two-factor 7-level uniform design scheme was applied.Separate treatment of each factor was performed for single effect test.Then,it analyzed the effects of acid and aluminum stress under soilless culture on the water content of plant during the growth of Ginkgo biloba L.seedlings,so as to provide a theoretical basis for studying the connection between plant water and physiological stress.The results showed that the water content of G.biloba plants declined with the decrease of p H,but the water content of G.biloba plants declined with the increase in the concentration of aluminum treatment,indicating that the stronger the acid and aluminum stress,the lower the water content of the plants,affecting the normal absorption of water content of G.biloba plants,accordingly leading to lack of water.According to the analysis of the degree of influence on the water content,p H is the first factor influencing the growth of G.biloba plants.If p H is lower than 3.5,G.biloba plants will not growth,while p H is higher than 5.0,aluminum has no significant effect on the water content of G.biloba plants.In conclusion,the water content of G.biloba plants can be used as an essential indicator for acid and aluminum stress. | Qiuliang YU Huilan SU Chunlan CHEN Zhongfang LI | 2018 | Asian Agricultural Research2018，10，7： | 1 |
| 12 | Bird nest-like zinc oxide nanostructures for sensitive electrochemical glucose biosensor显示文摘In this research,a novel bird nest-like zinc oxide(BN-ZnO)nanostructures were prepared by a simple solvothermal method.A sensitive electrochemical glucose biosensor was for the first time developed based on the immobilization of glucose oxidase(GOx)on nanostructured BN-ZnO modified electrode.The BN-ZnO nanostructure and the resultant biosensor were characterized by scanning electron microscope,X-ray diffraction spectroscopy,Fourier transform infrared spectroscopy,and electrochemical impedance spectroscopy.BN-ZnO nanostructures have large specific surface area and can load large amounts of GOx molecules.Meanwhile,BN-ZnO provides an excellent microenvironment to retain the native bioactivity of enzymes and to promote direct electron transfer between GOx and electrode surface.The proposed biosensor shows a wide linear range of 0.005–1.6 mmol/L,high sensitivity of15.6 mA L mol^(-1)cm^(-2)with a low detection limit of 0.004 mmol/L.The resulting biosensor also shows excellent selectivity,acceptable stability and reproducibility,and can be successfully applied in the detection of glucose in human serum samples at-0.37 V. | Feng Shi Jinming Xu Zhongfang Hu Chuanli Ren Yadong Xue Yongcai Zhang Juan Li Chengyin Wang Zhanjun Yang | 2021 | Chinese Chemical Letters2021，32，10： | 1 |
| 13 | Melatonin modulates the effects of diethylstilbestrol (DES) on the anterior pituitary of the female Wistar rat显示文摘 | Zhao Weijiang Shi Zhongfang Yuan Fang Li Guilin Sun Yilin Zhang Yazhuo Wang Zhongcheng | 2010 | Folia Histochemica et Cytobiologica2010，，2： | 1 |
| 14 | National multi-purpose regional geochemical survey in China显示文摘 | Min Li Xiaohuan Xi Guiyi Xiao Hangxin Cheng Zhongfang Yang Guohua Zhou Jiayu Ye Zhonghui Li | 2013 | Journal of Geochemical Exploration2013，，： | 1 |
| 15 | Annual net input fluxes of heavy metals of the agro-ecosystem in the Yangtze River delta, China显示文摘 | Qingye Hou Zhongfang Yang Junfeng Ji Tao Yu Guoguang Chen Juan Li Xueqi Xia Ming Zhang Xuyin Yuan | 2013 | Journal of Geochemical Exploration2013，，： | 1 |
| 16 | Tuning electronic proDerties of Germanane layers by external electric field and biaxial tensile strain: a computational study显示文摘 | Li Yafei Che Zhongfang | 2014 | Journal of Physical Chemistry C2014，118，2： | 1 |
| 17 | Study on Shaopu Liu, the resonance ttering spectra of the interactionsof hydrochloride and chlorpromazinehydrochloride with 12-tungstophosphoric acid and their analytical applications显示文摘 | Pei Zhongfang Liu nonlinear sca promethazine li Chen and Yanqi Xiaoli Hu Song | 2010 | Spectrochimica ncta Part A:2010，77，1： | 1 |
| 18 | Bacteriophages in water pollution control:Advantages and limitations显示文摘Wastewater is a breeding ground for many pathogens,which may pose a threat to human health through various water transmission pathways.Therefore,a simple and effective method is urgently required to monitor and treat wastewater.As bacterial viruses,bacteriophages(phages)are the most widely distributed and abundant organisms in the biosphere.Owing to their capacity to specifically infect bacterial hosts,they have recently been used as novel tools in water pollution control.The purpose of this review is to summarize and evaluate the roles of phages in monitoring pathogens,tracking pollution sources,treating pathogenic bacteria,infecting bloom-forming cyanobacteria,and controlling bulking sludge and biofilm pollution in wastewater treatment systems.We also discuss the limitations of phage usage in water pollution control,including phage-mediated horizontal gene transfer,the evolution of bacterial resistance,and phage concentration decrease.This review provides an integrated outlook on the use of phages in water pollution control. | Mengzhi Ji Zichen Liu Kaili Sun Zhongfang Li Xiangyu Fan Qiang Li | 2021 | Frontiers of Environmental Science & Engineering2021，15，5： | 1 |
| 19 | Growing in times of grief: Attachment modulates bereaved adults’ posttraumatic growth after losing a family member to cancer显示文摘 | Wei Xu Zhongfang Fu Li He Dominik Schoebi Jianping Wang | 2015 | Psychiatry Research2015，，： | 1 |
| 20 | The interaction between furosemide-palladium (Ⅱ) chelate and basic triphenylmethane dyes by resonance Rayleigh scattering spectra and resonance non-linear scattering spectra and their analytical applications显示文摘In pH 4.5-7.0 Britton-Robinson buffer solution,furosemide (FUR) reacted with Pd (II) to form a 1:1 anionic chelate.This chelate could further react with such basic triphenylmethane dyes (BTPMD) as ethyl violet (EV),crystal violet (CV),methyl violet (MV),methyl green (MeG) and brilliant green (BG) to form 1:1 ion-association complexes.This not only resulted in the change of absorption spectra,but also led to the significant enhancement of resonance Rayleigh scattering (RRS),second-order scattering (SOS) and frequency doubling scattering (FDS).The maximum RRS wavelengths were located at 324 nm for the EV,CV and MV system,and 340 nm for the BG and MeG system.The maximum SOS wavelengths were located at 550 nm for the EV,CV,BG and MeG system,and 530 nm for the MV system.The maximum scattering peaks of all the systems were at 392 nm for FDS.The enhanced RRS,SOS and FDS intensities were directly proportional to the concentration of FUR.The detection limits for the different dye systems were 0.3-4.9 ng mL-1 for the RRS method,3.2-33.1 ng mL-1 for the SOS method and 9.0-85.7 ng mL-1 for the FDS method.These methods could be used for the determination of trace amounts of FUR.The effects of the formation of ternary ion-association complexes on the spectral characteristics and intensities of absorption,RRS,SOS and FDS have been investigated.The optimum conditions of these reactions,the influencing factors and the analytical properties have been tested.The influences of coexisting substances were tested by RRS method and the results showed that this method exhibited a high sensitivity.Based on the aforementioned research,the highly sensitive,simple and rapid methods for the determination of trace amounts of FUR by resonance light scattering technique have been established,which could be applied to the determination of FUR in tablet,injection,human serum and urine samples.The composition and structure of the ternary ion-association complex and the reaction mechanism were discussed. | LI CuiXia,LIU ShaoPu,LIU ZhongFang & HU XiaoLi Key Laboratory on Luminescence and Real-Time Analysis,Ministry of Education School of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China | 2010 | Science China Chemistry2010，53，8： | 1 |
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