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| 1 | Aberrant seed development in Litchi chinensis is associated with the impaired expression of cell wall invertase genes显示文摘Cell wall invertase(CWIN)are known to play important roles in seed development.However,most reports to date have focused on a single gene family member,and have mainly investigated CWIN functions during the filling stage of seed development.In this study,we found significant lower levels of CWIN protein and activity associated with seed abortion in the Litchi chinensis cultivar“Nuomici.”We identified five litchi CWIN genes and observed that the expression of LcCWIN5 was limited to the flower tissues and decreased sharply with fruit development.Silencing of LcCWIN5 expression before 28 DAA(cell division stage)resulted in perturbed liquid endosperm development,smaller seeds,and higher seed abortion rate,while silencing after 28 DAA(filling stage)had no effect on seed development.In contrast,LcCWIN2 was mostly expressed in the funicle and seed coat,and increased with fruit development.Decreased LcCWIN2 expression and CWIN activity during early seed filling coincided with smaller seeds in the cultivar“Feizixiao.”Silencing of LcCWIN2 caused a reduction in the seed size without inducing seed abortion.We propose that CWIN activity in seed maternal tissues during cell division stage is likely due to LcCWIN5 expression,which regulates early seed development.On the other hand,CWIN activity during the filling stage is due to the expression of LcCWIN2,which may promote carbon import by creating a sucrose gradient.Comparable LcCWIN5 expression,but much lower CWIN activity,detected in the funicle of“Nuomici”is consistent with post-translational regulation. | Jieqiong Zhang Zichen Wu Fuchu Hu Lian Liu Xuming Huang Jietang Zhao Huicong Wang | 2018 | Horticulture Research2018，5，1： | 4 |
| 2 | High-Performance Photo-Modulated Thin-Film Transistor Based on Quantum dots/Reduced Graphene Oxide Fragment-Decorated ZnO Nanowires显示文摘In this paper, a photo-modulated transistor based on the thin-film transistor structure was fabricated on the flexible substrate by spin-coating and magnetron sputtering. A novel hybrid material that composed of Cd Se quantum dots and reduced graphene oxide(RGO) fragment-decorated ZnO nanowires was synthesized to overcome the narrow optical sensitive waveband and enhance the photo-responsivity. Due to the enrichment of the interface and heterostructure by RGO fragments being utilized, the photo-responsivity of the transistor was improved to 2000 AW^(-1) and the photo-sensitive wavelength was extended from ultraviolet to visible. In addition, a positive back-gate voltage was employed to reduce the Schottky barrier width of RGO fragments and ZnO nanowires. As a result, the amount of carriers was increased by 10 folds via the modulation of back-gate voltage. With these inherent properties, such as integrated circuit capability and wide optical sensitive waveband, the transistor will manifest great potential in the future applications in photodetectors. | Zhi Tao Yi-an Huang Xiang Liu Jing Chen Wei Lei Xiaofeng Wang Lingfeng Pan Jiangyong Pan Qianqian Huang Zichen Zhang | 2016 | Nano-Micro Letters2016，8，3： | 2 |
| 3 | Targeted minicircle DNA delivery using folate–poly(ethylene glycol)–polyethylenimine as non-viral carrier显示文摘 | Chao Zhang Shijuan Gao Wei Jiang Song Lin Fusheng Du Zichen Li Wenlin Huang | 2010 | Biomaterials2010，，23： | 1 |
| 4 | An im- proved symplectic precise integration method for analysis of the rotating rigid-flexible coupled system 显示文摘 | HUANG Yongan DENG Zichen YAO Linxiao | 2007 | Journal of Sound and Vibration2007，299，12： | 1 |
| 5 | Discharge rate consistency of each channel for UAV-based pneumatic granular fertilizer spreader显示文摘Unmanned aerial vehicles(UAVs)are widely being used to spread granular fertilizer in China.Granular fertilizer spreaders equipped with UAVs are mainly centrifugal disc-type and pneumatic.The multichannel pneumatic granular fertilizer spreaders(MPGFSs)have a banded fertilizer deposition distribution pattern,which are more suitable for variable rate fertilization with high precision requirement than the circular deposition distribution pattern of disc-type granular fertilizer spreaders(DGFSs).However,the existing MPGFS has the disadvantage of inconsistent discharge rate of each channel,which affects the uniformity of fertilization.In order to explore the causes of inconsistent discharge rate of each channel,the discrete element method(DEM)and bench test were performed to analysis the discharge process of the fluted roller fertilizing apparatus and distribution of fertilizer in axial direction of fluted roller.The computational fluid dynamics(CFD)was used to simulate the airflow field of pneumatic system to analyze the influence of airflow on the movement of fertilizer particles.The simulation results of the discharge process of the fluted roller fertilizing apparatus showed that the filling velocity at the axial ends of the fluted roller fertilizing apparatus was lower than that of the middle.The reason was that the filling capacity was weak near the wall.The simulated results of the airflow field without partitions showed that the airflow provided by the axial flow fan was rotational,and this caused the particles to move irregularly in the throat,resulting in inconsistency discharge rate of each channel.Based on the analysis of reasons of inconsistent discharge rate of each channel,a MPGFS with partitions in the throat was developed.The discharge rate bench tests were carried out to optimize the partition spacing parameters,and fertilization test was performed to test the performance of the improved MPGFS.The discharge rate test results showed better consistency with partition.The coefficient of variation(CV)of the discharge rate of each channel was 20.16%without the partition and 7.70%with the optimal partition.The fertilizer spreading uniformity bench test results shown that the CV of spreading uniformity of MPGFS without partitions was 15.32%,and that MPGFS with partitions was 8.69%.The partitions design was beneficial to improve the consistency of each channel discharge rate and the uniformity of fertilization.The finding can provide a strong reference to design the MPGFS. | Xunwei Wang Rui Jiang Zhiyan Zhou Cancan Song Xiwen Luo Ruifeng Bao Zichen Lyu Junhao Huang Jianqin Lin | 2023 | International Journal of Agricultural and Biological Engineering2023，16，4： | 1 |
| 6 | Disease resistance conferred by components of essential chrysanthemum oil and the epigenetic regulation of OsTPS1显示文摘The sesquiterpene alpha-bisabolol is the predominant active ingredient in essential oils that are highly valued in the cosmetics industry due to its wound healing,anti-inflammatory,and skin-soothing properties.Alpha-bisabolol was thought to be restricted to Compositae plants.Here we reveal that alpha-bisabolol is also synthesized in rice,a non-Compositae plant,where it acts as a novel sesquiterpene phytoalexin.Overexpressing the gene responsible for the biosynthesis of alpha-bisabolol,Os TPS1,conferred bacterial blight resistance in rice.Phylogenomic analyses revealed that alpha-bisabolol-synthesizing enzymes in rice and Compositae evolved independently.Further experiments demonstrated that the natural variation in the disease resistance level was associated with differential transcription of Os TPS1 due to polymorphisms in its promoter.We demonstrated that Os TPS1 was regulated at the epigenetic level by JMJ705 through the methyl jasmonate pathway.These data reveal the cross-family accumulation and regulatory mechanisms of alpha-bisabolol production. | Chuansong Zhan Long Lei Hao Guo Shen Zhou Congping Xu Zixin Liu Zichen Wu Yuan Deng Yuanyuan Miao Yu Han Meng Zhang Hua Li Sishu Huang Chenkun Yang Feng Zhang Yufei Li Ling Liu Xianqing Liu Hafiz Muhammad Khalid Abbas Alisdair R.Fernie Meng Yuan Jie Luo | 2023 | Science China(Life Sciences)2023，66，5： | 1 |
| 7 | Automated Variable Weighting in k - Means Type Cluste- ring显示文摘 | Huang Joshua Zhexue K Ng Michael Rong Hongqiang and Li Zichen | 2005 | IEEE Transactions on Pattern Analysis and Machine In- telligence2005，27，5： | 1 |
| 8 | Vesicles with superior stability at high temperature 显示文摘 | YAN YUN HUANG JIANBIN LI ZICHEN | | J Phys Chem B0，7，： | 1 |
| 9 | Aggregates transition depending on the concentration in the cationic Bolaamphiphile/SDS mixed systems显示文摘 | Huang Jianbin Li Zichen | 2003 | Langmuir2003，19，： | 1 |
| 10 | An improved symplectic precise integration method for analysis of the rotating rigidflexible coupled system 显示文摘 | Huang Yong'an Deng Zichen Yao Linxiao | 2007 | Journal of Sound and Vibration2007，，299： | 1 |
| 11 | Aggregates transition depending on the concentration in the cationic bolaamphiphile/SDS mixed systems显示文摘 | YAN Yun HUANG Jianbin LI Zichen | 2003 | Langmuir2003，19，3： | 1 |
| 12 | Water-Driven Malleable,Weldable and Eco-Friendly Recyclable Carbon Fiber Reinforced Dynamic Composites显示文摘Traditional carbon fiber-reinforced polymers based on thermoset matrix have been extensively used in the fields of wind turbine blades,automotive sector,and aerospace,among many others.However,there is still a major challenge of recycling those polymers due to the high cost and adverse impacts on the environment.In this work,we apply a polyimine network as matrix,which possess considerable tensile and thermal properties,to prepare the carbon fiber reinforced polyimine materials with trifluoromethyl diphenoxybenzene units(CFRFP)using a prepreg-based compression molding method.The CFRFP can be reshaped or reprocessed by heat or with water rapidly,and exhibited multifunction,including welding,chemical recycling,etc.These unique findings gained from our study will facilitate the manufacturing capability and enrich the types of fiber-reinforced composites. | Ping Yu Qirui Huang Yi Wang Wan Peng Zichen Jia Haiyue Wang Juanjuan Ma Chunyu Wang Xuzhou Yan | 2024 | Chinese Journal of Chemistry2024，42，5： | 0 |
| 13 | Static and dynamic evaluations of acoustic positioning system using TDMA and FDMA for robots operating in a greenhouse显示文摘Acoustic positioning system has great potential to be applied in a greenhouse due to its centimeter-level accuracy,low cost,and ability of extensive greenhouse coverage.Spread Spectrum Sound-based local positioning system(SSSLPS)was proposed to be a navigation tool for multiple agricultural robots by the authors'research team.However,to increase the system capacity for positioning multiple robots in a greenhouse,the near-far problem caused by the interference between speakers needs to be overcome.The use of different access methods,Time Division Multiple Access(TDMA)or Frequency Division Multiple Access(FDMA),is essential in the SSSLPS system for solving the near-far problem.The static positioning in a greenhouse was first evaluated by setting different parameters to determine the optimal signal setting for a dynamic experiment.From that,the moving robot tests were added with a motion capture system and tested the performance of TDMA and FDMA.The results demonstrated that TDMA can be used in a stationary sound-based positioning system with 12.2 mm accuracy,but it has a time delay problem in dynamic positioning.A simulation was designed to mimic the position error increases with different moving speeds.Although FDMA has the sound damping problem in high-frequency regions creating a peak detection issue,it achieved a higher accuracy with an average position error of 62.1 mm compared to 180.3 mm of TDMA.This study shows that the TDMA method is suitable for static measurements,while the FDMA method is suitable for measuring dynamic objects and controlling mobile robots. | Lok Wai Jacky Tsay Tomoo Shiigi Xunyue Zhao Zichen Huang Keiichiro Shiraga Tetsuhito Suzuki Yuichi Ogawa Naoshi Kondo | 2022 | International Journal of Agricultural and Biological Engineering2022，15，5： | 0 |
| 14 | Targeting LncRNA LLNLR-299G3.1 with antisense oligonucleotide inhibits malignancy of esophageal squamous cell carcinoma cells in vitro and in vivo显示文摘Accumulating evidence has indicated that long non-coding RNAs(lncRNAs)play critical roles in the development and progression of cancers,including esophageal squamous cell carcinoma(ESCC).However,the mechanisms of lncRNAs in ESCC are still incompletely understood and therapeutic attempts for in vivo targeting cancer-associated lncRNA remain a challenge.By RNA-sequencing analysis,we identified that LLNLR-299G3.1 was a novel ESCC-associated lncRNA.LLNLR-299G3.1 was up-regulated in ESCC tissues and cells and promoted ESCC cell proliferation and invasion.Silencing of LLNLR-299G3.1 with ASO(antisense oligonucleotide)resulted in opposite effects.Mechanistically,LLNLR-299G3.1 bound to cancerassociated RNA binding proteins and regulated the expression of cancer-related genes,including OSM,TNFRSF4,HRH3,and SSTR3.ChIRP-seq(chromatin isolation by RNA purification and sequencing)revealed that these genes contained enriched chromatin binding sites for LLNLR-299G3.1.Rescue experiments confirmed that the effects of LLNLR-299G3.1 on ESCC cell proliferation were dependent on interaction with HRH3 and TNFRSF4.Therapeutically,intravenous delivery of placental chondroitin sulfate A binding peptide-coated nanoparticles containing antisense oligonucleotide(pICSA-BP-ANPs)strongly inhibited ESCC tumor growth and significantly improved animal survival in vivo.Overall,our results suggest that LLNLR-299G3.1 promotes ESCC malignancy through regulating gene-chromatin interactions and targeting ESCC by pICSA-BP-ANPs may be an effective strategy for the treatment of lncRNA-associated ESCC. | LI TIAN YONGYI HUANG BAOZHEN ZHANG YI SONG LIN YANG QIANQIAN CHEN ZHENG WANG YILING WANG QIHAN HE WENHAN YANG SHUYONG YU TIANYU LU ZICHEN LIU KAIPING GAO XIUJUN FAN JIAN SONG RIHONG ZHAI | 2023 | Oncology Research2023，31，4： | 0 |
| 15 | Development of UAV-based shot seeding device for rice planting显示文摘In order to realize the high-quality row seeding operation with unmanned aerial vehicle(UAV)in paddy field,a shot seeding device that can sow five rows of pelleted rice seeds at the same time was designed.The shot seeding device mainly consists of an external grooved wheel seed metering device and five shot seeding modules.The designed external grooved wheel seed metering device can take the seeds out of the seed box and divide the seeds into five parts.The shot seeding module can accelerate the pelleted rice seeds to reduce the impact of the UAV wind on the direction of seed movement.Furthermore,an angle adjustment mechanism for the shot seeding module was used to change the row spacing.The seed metering device test verified that when the speed of the seed metering wheel was 15 r/min,the coefficient of variation of the discharge rate of each row(C.V1)and total seed discharge rate stability(C.V2)were 1.70%and 1.04%,respectively.Image processing technique was used to test the UAV seeding performance.The distribution characteristics of seeds on the ground showed that the number of seeds in each row gradually increased from both sides to the middle in the width direction.According to the statistics,there were 60%-70%of the seeds in each row in the 100 mm width range.The field test showed that when the working height was 1.5 m and the seeding quantity was 38.56 kg/hm^(2),the performance of sowing in rows was obvious,the deviation rate of seeding quantity was 1.89%.After 16 d of sowing,the seeds’emergence rate was stable,and the average emergence rate was 82.63%and the yield was 6775.50 kg/hm^(2). | Wei Liu Shuaishuai Zou Xuelang Xu Qingyu Gu Weizhuo He Jun Huang Junhao Huang Zichen Lyu Jianqin Lin Zhiyan Zhou Rui Jiang Xiwen Luo | 2022 | International Journal of Agricultural and Biological Engineering2022，15，6： | 0 |
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