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| 1 | Identification of gamyb-4 and Analysis of the Regulatory Role of GAMYB in Rice Anther Development显示文摘In higher plants,male reproductive development is a complex biological process that includes cell division and differentiation,cell to cell communication etc.,while the mechanism underlying plant male reproductive development remains less understood.GAMYB encodes a gibberellins acid(GA) inducible transcription factor that is required for the early anther development in rice(Oryza sativa L.).Here,we report the isolation and characterization of a new allele gamyb-4 with a C base deletion in the second exon(+2308),causing a frame shift and premature translational termination.Histological analysis showed that gamyb-4 developed abnormal enlarged tapetum and could not undergo normal meiosis.To understand the regulatory role of GAMYB,we carried out quantitative reverse transcription-polymerase chain reaction analysis and comparison of microarray data.These results revealed that the expression of TDR(TAPETUM DEGENERATION RETARDATION),a tapetal cell death regulator,was downregulated in gamyb-4 and udt1(undeveloped tapetum1).While the GAMYB expression was not obviously changed in tdr and udt1-1,and no apparent expression fold change of UDT1 in tdr and gamyb-4,suggesting that TDR may act downstream of GAMYB and UDT1,and GAMYB and UDT1 work in parallel to regulate rice early anther development.This work is helpful in understanding the regulatory network in rice anther development. | Zhenhua Liu Wenjie Bao Wanqi Liang Jingyuan Yin Dabing Zhang | 2010 | Journal of Integrative Plant Biology2010，52，7： | 35 |
| 2 | 水稻隐性核雄性不育基因研究进展及育种应用探讨显示文摘水稻隐性核雄性不育材料在杂交育种和水稻生产上都具有十分重要的意义。但是,由于缺乏有效的保持和繁殖技术体系,该类不育材料一直未能在生产上获得充分利用。而现代分子与生物技术的快速发展为这些隐性核雄性不育基因的有效利用提供了机会。综述了目前已克隆的水稻隐性核雄性不育基因的研究进展,并总结了玉米、番茄和拟南芥等材料中存在的隐性核雄性不育基因。我们期望利用这些基因信息和新的生物技术手段(例如定点突变技术),创制更多的水稻不育基因,同时利用新的基因工程手段——种子生产技术(SPT)体系,并结合传统的育种方法,有效地解决水稻隐性核雄性不育系的保持和繁殖难题,开创新的杂交育种途径,拓宽水稻杂种优势的利用空间。 | 马西青 方才臣 邓联武 万向元 | 2012 | 中国水稻科学2012，26，5： | 12 |
| 3 | The polyketide synthase OsPKS2 is essential for pollen exine and Ubisch body patterning in rice显示文摘Lipid and phenolic metabolism are important for pollen exine formation. In Arabidopsis, polyketide synthases(PKSs) are essential for both sporopollenin biosynthesis and exine formation. Here, we characterized the role of a polyketide synthase(OsPKS2) in male reproduction of rice(Oryza sativa). Recombinant OsPKS2 catalyzed the condensation of fatty acyl-CoA with malonyl-CoA to generate triketide and tetraketide α-pyrones, the main components of pollen exine. Indeed, the ospks2 mutant had defective exine patterning and was male sterile. However, the mutant showed no significant reduction in sporopollenin accumulation. Compared with the WT(wild type), ospks2 displayed unconfined and amorphous tectum and nexine layers in the exine, and less organized Ubisch bodies. Like the pksb/lap5 mutant of the Arabidopsis ortholog, ospks2 showed broad alterations in the profiles of anther-related phenolic compounds.However, unlike pksb/lap5, in which most detected phenolics were substantially decreased, ospks2 accumulated higher levels of phenolics. Based on these results and our observation that OsPKS2 is unable to fully restore the exine defects in the pksb/lap5, we propose that PKS proteins have functionally diversified during evolution.Collectively, our results suggest that PKSs represent a conserved and diversified biochemical pathway for anther and pollen development in higher plants. | Xiaolei Zhu Jing Yu Jianxin Shi Takayuki Tohge Alisdair R.Fernie Sagit Meir Asaph Aharoni Dawei Xu Dabing Zhang Wanqi Liang | 2017 | Journal of Integrative Plant Biology2017，59，9： | 9 |
| 4 | EMS诱发菊花突变类型及重要性状的分子鉴定显示文摘利用化学诱变剂甲基磺酸乙酯(ethyl methane sulfonate,EMS)处理菊花品种‘神马’茎尖以获得各种突变类型。对菊花的生物学性状及观赏性状进行调查,结果表明,突变群体的表型变异率达到6.31%,获得了菊花株高、叶片、开花期、花序以及花瓣等观赏性状的突变体。表型变异类型多样,特别是发现了自然突变或人工诱变中少见的开花期提早突变体和花器官的突变类型,如花苞片消失或易位、舌状花和筒状花增加或缺失等。本试验所用EMS构建的菊花突变群体变异类型丰富,且ISSR标记鉴定结果显示标记条带确实出现了差异,这表明突变植株的基因发生了变化。这为菊花新品种培育和功能基因组学的研究奠定了基础。 | 蔡海燕 温立柱 郑成淑 李莹莹 孙霞 孙宪芝 | 2013 | 山东农业大学学报(自然科学版)2013，44，2： | 7 |
| 5 | 水稻雄性发育功能基因的发掘及应用显示文摘水稻雄配子体发育包含花药细胞分化、绒毡层细胞程序性死亡、花粉壁形态建成和受精等连续的发育过程,是生殖发育基础理论研究和水稻育种特别是杂交育种的关键过程。现重点介绍近年来国内外水稻雄性发育分子调控机制的最新研究进展,并概述雄性不育系在杂种优势利用、杂交不亲和等重要农艺性状控制方面的理论基础。 | 王多祥 祝万万 袁政 张大兵 | 2016 | 生命科学2016，28，10： | 6 |
| 6 | Less and shrunken pollen 1(LSP1) encodes a member of the ABC transporter family required for pollen wall development in rice(Oryza sativa L.)显示文摘Pollen fertility is an agronomic trait that strongly influences rice yield. Recent studies have revealed that the development of the pollen wall is required for pollen fertility and is regulated by several genes. However, the mechanisms underlying pollen and pollen wall development in rice remain largely unknown. In the present study, a point mutation in a gene on chromosome 1 was identified that resulted in the production of less and shrunken pollen(LSP) and led to defects in pollen wall formation. This gene was named LSP1 and was found to encode a member of the adenosine triphosphate-binding cassette(ABC)transporter G subfamily, OsABCG3. Two other loss-of-function mutants of LSP1/OsABCG3,generated using CRISPR/Cas9 technology, showed the same male sterile phenotype. The LSP1/OsABCG3 gene showed a spatio-temporal expression pattern in the developing anthers, and is an ortholog of the Arabidopsis genes At ABCG1 and At ABCG16, which play an important role in pollen wall development. Mutation of LSP1/OsABCG3 affected the expression of several genes involved in pollen and pollen wall formation. These results suggest that LSP1/OsABCG3 is critical for normal pollen fertility and shed light on the molecular mechanisms underlying rice pollen wall development. | Tao Luo Ting Zou Guoqiang Yuan Zhiyuan He Wenjie Li Yang Tao Miaomiao Liu Dan Zhou Hongfeng Zhao Jun Zhu Yueyang Liang Qiming Deng Shiquan Wang Aiping Zheng Huainian Liu Lingxia Wang Ping Li Shuangcheng Li | 2020 | The Crop Journal2020，8，3： | 4 |
| 7 | 水稻窄叶突变体相关基因的研究进展显示文摘片是作物光合作用与呼吸作用的重要场所,同时也作为蒸腾作用的主要调节器官。其形态构成与转录因子、激素以及小RNA相关并受其调控,深入探究叶片发育的网络调控机理对高光效育种工作具有非常重要的意义。窄叶性状是叶形态的一种,在超高产株型育种中偏向选择窄叶,窄叶是株型构成非常重要方面,而株型与作物产量又密不可分,因此水稻窄叶作为一种叶型农艺性状,在株型育种中具有非常重要的应用价值。本研究归纳总结了至今为止已定位克隆的水稻窄叶突变体相关基因及其调控机理,并进一步探讨窄叶突变体基因的一因多效型,分析其在育种中的应用,以期为水稻株型育种中叶型性状的选择提供理论依据。 | 潘境涛 谢红卫 钱明娟 徐斌 蔡耀辉 彭晓珏 | 2017 | 分子植物育种2017，15，12： | 3 |
| 8 | 植物雄性发育与减数分裂研究现状和展望显示文摘植物生殖发育过程直接影响许多重要农作物的产量。雄性发育与减数分裂为植物生殖发育过程中的重要过程,对其调控机理的研究不仅有助于理解生殖发育的分子机制,也可以为农作物的遗传育种提供理论依据。近些年来,我国科学家在此领域取得了一系列高水平的研究成果,主要包括植物花药中关键细胞的命运决定、绒毡层细胞和小孢子发生的分子机制、减数分裂同源染色体相互作用的分子机理和花粉萌发及伸长的基因调控等等。本文对我国科学家所取得的诸多成就进行了简要的回顾,并提出了对该领域发展的一些思考和看法。 | 陆平利 马红 | 2016 | 中国基础科学2016，18，2： | 0 |
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