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1Plant abiotic stress response and nutrient use efficiency显示文摘Abiotic stresses and soil nutrient limitations are major environmental conditions that reduce plant growth,productivity and quality.Plants have evolved mechanisms to perceive these environmental challenges,transmit the stress signals within cells as well as between cells and tissues,and make appropriate adjustments in their growth and development in order to survive and reproduce.In recent years,significant progress has been made on many fronts of the stress signaling research,particularly in understanding the downstream signaling events that culminate at the activation of stress-and nutrient limitation-responsive genes,cellular ion homeostasis,and growth adjustment.However,the revelation of the early events of stress signaling,particularly the identification of primary stress sensors,still lags behind.In this review,we summarize recent work on the genetic and molecular mechanisms of plant abiotic stress and nutrient limitation sensing and signaling and discuss new directions for future studies.Zhizhong Gong Liming Xiong Huazhong Shi Shuhua Yang Luis R.Herrera-Estrella Guohua Xu Dai-Yin Chao Jingrui Li Peng-Yun Wang Feng Qin Jigang Li Yanglin Ding Yiting Shi Yu Wang Yongqing Yang Yan Guo Jian-Kang Zhu 2020Science China(Life Sciences)2020,63,5:94
2Molecular Regulation of Plant Responses to Environmental Temperatures显示文摘Temperature is a key factor governing the growth and development,distribution,and seasonal behavior of plants.The entireplant life cycle is affected by environmental temperatures.Plants grow rapidly and exhibit specific changes in morphology under mild average temperature conditions,a response termed thermomorphogenesis.When exposed to chilling or moist chilling low temperatures,flowering or seed germination is accelerated in some plant species;these processes are known as vernalization and cold stratification,respectively.Interestingly,once many temperate plants are exposed to chilling temperatures for some time,they can acquire the ability to resist freezing stress,a process termed cold acclimation.In the face of global climate change,heat stress has emerged as a frequent challenge,which adversely affects plant growth and development.In this review,we summarize and discuss recent progress in dissecting them olecular mechanism sregulating plant thermomorphogenesis,vernalization,and responses to extreme temperatures.We also discuss the remaining issues that are crucial for understanding the interactions between plants and temperature.Yanglin Ding Yiting Shi Shuhua Yang 2020Molecular Plant2020,13,4:40
3AP2/ERF转录因子调控植物非生物胁迫响应研究进展显示文摘低温、干旱、高盐和缺氧等多种不良环境影响植物的生长发育,植物通过长期进化形成复杂的调节机制来适应这些不利条件。AP2/ERF是植物特有的转录因子,在各种胁迫响应过程中发挥关键调控作用。近年来,越来越多的研究表明,植物激素介导的信号级联通路与逆境胁迫响应关系密切,AP2/ERF转录因子可与激素信号转导协同形成交叉调控网络。许多AP2/ERF转录因子通过响应植物激素脱落酸和乙烯,激活依赖或不依赖于脱落酸和乙烯的胁迫响应基因的表达。此外,AP2/ERF转录因子参与赤霉素、细胞分裂素和油菜素内酯介导的生长发育和胁迫应答。该文简要综述了AP2/ERF转录因子的结构特征、转录调控、翻译后修饰、结合位点、协同互作蛋白及其参与调控依赖或不依赖激素信号转导途径的非生物胁迫响应研究进展,为解析不同AP2/ERF转录因子在调控激素和胁迫响应网络中的作用提供理论依据。洪林 杨蕾 杨海健 王武 2020植物学报2020,55,4:20
4Rice functional genomics:decades'efforts and roads ahead显示文摘Rice(Oryza sativa L.)is one of the most important crops in the world.Since the completion of rice reference genome sequences,tremendous progress has been achieved in understanding the molecular mechanisms on various rice traits and dissecting the underlying regulatory networks.In this review,we summarize the research progress of rice biology over past decades,including omics,genome-wide association study,phytohormone action,nutrient use,biotic and abiotic responses,photoperiodic flowering,and reproductive development(fertility and sterility).For the roads ahead,cutting-edge technologies such as new genomics methods,high-throughput phenotyping platforms,precise genome-editing tools,environmental microbiome optimization,and synthetic methods will further extend our understanding of unsolved molecular biology questions in rice,and facilitate integrations of the knowledge for agricultural applications.Rongzhi Chen Yiwen Deng Yanglin Ding Jingxin Guo Jie Qiu Bing Wang Changsheng Wang Yongyao Xie Zhihua Zhang Jiaxin Chen Letian Chen Chengcai Chu Guangcun He Zuhua He Xuehui Huang Yongzhong Xing Shuhua Yang Daoxin Xie Yaoguang Liu Jiayang Li 2022Science China(Life Sciences)2022,65,1:12
5植物抗氧化动态平衡研究进展显示文摘植物在生长发育的过程中会产生代谢副产物活性氧,其含量在植物生长过程中起双重作用。适量的活性氧可提高植物对逆境胁迫的耐受性,但是过量的活性氧会诱发氧化猝发反应,严重影响植物的生长发育。因此,提高植物的抗氧化能力对于提高植物的抗逆能力来说显得尤为重要,该方面的研究也成为近年来逆境生物学的一大热点。植物体为了应对逆境环境造成的活性氧动态失衡,进化出了含酶和非酶组分的抗氧化系统。本文主要介绍了参与高等植物活性氧代谢的相关物质,对近年来国内外报道的代谢途径进行了综述,为提高植物的抗逆能力提供参考依据。高媛 薛艳红 刘士平 2019生物资源2019,41,1:12
6基于转录组测序技术挖掘薄荷幼苗响应干旱高温胁迫基因显示文摘本文以薄荷为研究对象,以蒸馏水、25℃为非胁迫对照,用聚乙二醇6000(PEG6000)模拟干旱胁迫,用光照培养箱设定40℃为高温胁迫,两种胁迫条件叠加为干旱-高温胁迫,各设3个生物学重复。处理2 h后,将对薄荷新叶进行取样,并快速放入液氮中速冻,之后放进-80℃下保存,随后进行转录组高通量技术测序(RNA-seq)。结果表明,与对照比较,干旱胁迫后产生4 577个差异表达基因(differentially expressed genes, DEGs),干旱-高温胁迫后产生21 163个DEGs;两种胁迫条件有2 141个共同DEGs,其中共同上调的有1 219个,共同下调的有798个,这些共同差异表达基因既可以响应干旱单一胁迫,也可以响应干旱-高温复合胁迫,且以上调为主;GO功能富集和KEGG功能富集分析表明,上调的主要涉及到植物激素信号转导、MAPK信号通路、蛋白质丝氨酸/苏氨酸磷酸酶活性(protein serine/threonine phosphatase activity, PP2C)、半乳糖代谢、淀粉和蔗糖代谢等,其中对含氧化合物的反应和转录因子的调节很关键。进一步对这些共同上调差异表达基因进行挖掘,分析结果表明,POD、GSTU17、GSTU8、GSTL3X1、GSTTCHQDX1等能同时上调干旱、干旱-高温胁迫时的抗氧化酶的表达,另外还含有主要集中在NAC、AP2/ERF-ERF、WRKY、MYB-related、HSF等基因家族的共同上调转录因子;挑选7个抗氧化酶基因进行荧光定量PCR验证,基因的表达趋势与转录组测序结果一致。上述共同上调的抗氧化酶基因和转录因子为薄荷幼苗响应干旱单一和干旱-高温复合胁迫的抗逆分子机制提供了重要的生物信息学研究依据。朱丹 李林华 高焕 2020生命的化学2020,40,12:6
7非生物逆境胁迫下的种子萌发调控机制研究进展显示文摘受全球工业化和气候变化的影响,具有固着生长特性的植物面临着多种非生物逆境的威胁,而种子在各类非生物逆境胁迫下的顺利萌发,对植物的繁殖与地理分布、作物的产量与质量等极其重要。随着植物分子生物学研究的不断深入,非生物逆境协迫下种子萌发的调控机制研究取得了诸多进展。本文重点综述了在盐胁迫、极端温度(高温和低温)胁迫、干旱胁迫、荫蔽胁迫、渗透胁迫和淹涝胁迫等非生物逆境胁迫下,植物通过介导激素、活性氧及能量代谢通路调控种子萌发的分子机制,并讨论了三大通路之间的互作,展望了未来该领域的研究热点,以期总结种子萌发过程中响应非生物逆境胁迫的主要调节模块/基因功能,为农业生产及环境改造中的育种设计提供参考。徐佳慧 赵晓亭 毛凯涛 王国栋 谭伟明 舒凯 2021陕西师范大学学报(自然科学版)2021,49,3:6
8蛋白质翻译后修饰在ABA信号转导中的作用显示文摘脱落酸(ABA)是植物生长发育和逆境适应过程中非常关键的植物激素。植物响应ABA信号转导过程由信号识别、转导及响应级联完成,其中心转导途径由ABA受体RCAR/PYR/PYLs、磷酸酶PP2Cs、激酶SnRK2s、转录因子和离子通道蛋白构成。蛋白磷酸化、泛素化、类泛素化和氧化还原等翻译后修饰在ABA转导途径中起重要作用。该文综述了翻译后修饰在ABA信号转导中的作用。张静 侯岁稳 2019植物学报2019,54,3:5
9低磷胁迫对柱花草生长及抗氧化系统的影响显示文摘以基因型TF291柱花草为供试材料,分析低磷处理(5μmol/L)对柱花草生长、抗氧化物质及抗氧化保护酶的影响,探索柱花草抗氧化系统在低磷胁迫下的响应机制。结果表明:(1)与对照磷处理(250μmol/L)相比,低磷处理抑制了柱花草生长,其叶绿素浓度、最大光化学效率、地上部和根部生物量均显著降低(P<0.05)。(2)随着低磷处理时间的增加,15 d时叶片CAT、POD、SOD、ASP、PAL活性和类黄酮含量显著提升(P<0.05);30 d时叶片CAT、SOD、ASP、PPO活性和MDA、H_(2)O_(2)含量显著提高(P<0.05)。本研究结果可为进一步探索低磷胁迫下柱花草抗氧化系统的分子响应机制提供重要依据。王金鹏 廖丽 刘国道 罗丽娟 刘攀道 王志勇 2021热带作物学报2021,42,10:5
10Shaping polyploid wheat for success:Origins,domestication,and the genetic improvement of agronomic traits显示文摘Bread wheat(Triticum aestivum L.,AABBDD,2 n=6 x=42),which accounts for most of the cultivated wheat crop worldwide,is a typical allohexaploid with a genome derived from three diploid wild ancestors.Bread wheat arose and evolved via two sequential allopolyploidization events and was further polished through multiple steps of domestication.Today,cultivated allohexaploid bread wheat has numerous advantageous traits,including adaptive plasticity,favorable yield traits,and extended end-use quality,which have enabled its cultivation well beyond the ranges of its tetraploid and diploid progenitors to become a global staple food crop.In the past decade,rapid advances in wheat genomic research have considerably accelerated our understanding of the bases for the shaping of complex agronomic traits in this polyploid crop.Here,we summarize recent advances in characterizing major genetic factors underlying the origin,evolution,and improvement of polyploid wheats.We end with a brief discussion of the future prospects for the design of gene cloning strategies and modern wheat breeding.Jie Liu Yingyin Yao Mingming Xin Huiru Peng Zhongfu Ni Qixin Sun 2022Journal of Integrative Plant Biology2022,64,2:5
11Rheostatic Control of ABA Signaling through HOS15-Mediated OST1 Degradation显示文摘Dehydrating stresses trigger the accumulation of abscisic acid(ABA),a key plant stress-signaling hormone that activates Snf1-Related Kinases(SnRK2s)to mount adaptive responses.However,the regulatory circuits that terminate the SnRK2s signal relay after acclimation or post-stress conditions remain to be defined.Here,we show that the desensitization of the ABA signal is achieved by the regulation of OST1(SnRK2.6)protein stability via the E3-ubiquitin ligase HOS15.Upon ABA signal,HOS15-induced degradation of OST1 is inhibited and stabilized OST1 promotes the stress response.When the ABA signal terminates,protein phosphatases ABI1/2 promote rapid degradation of OST1 via HOS15.Notably,we found that even in the presence of ABA,OST1 levels are also depleted within hours of ABA signal onset.The unexpected dynamics of OST1 abundance are then resolved by systematic mathematical modeling,demonstrating a desensitizing feedback loop by which OST1-induced upregulation of ABI1/2 leads to the degradation of OST1.This model illustrates the complex rheostat dynamics underlying the ABA-induced stress response and desensitization.Akhtar Ali Jae Kyoung Kim Masood Jan Haris Ali Khan Irfan Ullah Khan Mingzhe Shen Junghoon Park Chae Jin Lim Shah Hussain Dongwon Baek Kai Wang Woo Sik Chung Vicente Rubio Sang Yeol Lee Zhizhong Gong Woe Yeon Kim Ray ABressan Jose MPardo Dae-Jin Yun 2019Molecular Plant2019,12,11:5
12水分及高温环境胁迫对薄荷幼苗活性氧代谢与总黄酮累积的影响显示文摘水资源紧缺和全球气候变暖趋势,使药用植物同时面临着水分及高温胁迫.本文以薄荷幼苗为研究对象,通过光照培养箱模拟胁迫环境,用聚乙二醇(PEG6000)模拟水分单一胁迫,设置40℃为高温单一胁迫,二者交互为复合胁迫,蒸馏水、25℃为对照,主要测定0~24 h超氧阴离子(O2-^-)产生速率,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性,过氧化氢(H2O2)、丙二醛(MDA)质量摩尔浓度,以及黄酮的质量分数变化.结果显示:水分及高温单一、复合胁迫处理下,O2-^-产生速率、H2O2质量摩尔浓度、SOD、POD和CAT活性表现为先升后降的趋势,单一胁迫MDA质量摩尔浓度为整体上升趋势,复合胁迫MDA先升高后降低;4~12 h之间,复合胁迫各检测值显著高于单一胁迫,表现为协同效应;24 h复合胁迫下所有检测值先于单一胁迫降低至(甚至低于)对照水平.可能是由于复合胁迫伤害大于单一胁迫伤害,随着时间的延长,率先突破薄荷幼苗忍受临界点导致死亡.黄酮累积的变化表现为从4 h上升至8 h质量分数最大,到12 h显著下降,与O2-^-产生速率、H2O2的质量摩尔浓度分别在4、8 h达到最大,到12 h显著降低的趋势一致,提示胁迫条件下黄酮的累积很有可能是因为活性氧的产生而上调的.研究表明:水分、高温胁迫可以通过诱导抗氧化酶来保护薄荷幼苗因活性氧的产生而导致的伤害,但是有限的;活性氧同时又可能提升幼苗黄酮的累积.因此,在实际生产应用中,不仅要考虑到胁迫的协同效应,还要考虑胁迫的时间效应,从而平衡植物“产量”与“质量”的最佳方案.朱丹 赵宝林 韩科学 赵晓 2020北京师范大学学报(自然科学版)2020,56,4:4
13根际微生物影响因素及其与植物互作研究进展显示文摘植物根际是植物与土壤互作的界面,定殖的微生物群落结构复杂,功能多样.植物在所有生长阶段均通过根际与土壤及其微生物进行复杂互作,环境与植物根际分泌物在很大程度上驱动根际微生物群落的变化.根际微生物与植物互作产生的复杂生态效应是土壤生态学研究的热点.综述了根际微生物群落的主要驱动因素,阐明了根际微生物增强植物营养吸收、抗病性、抗逆性的主要机制以及挥发性有机物介导的互作相关研究,以期明确根际微生物群落组装以及植物根际微生物互作机制及其生态效应,为农林业生产及生态文明建设提供参考.吴昌昊 刘敬泽 2022河北师范大学学报(自然科学版)2022,46,6:3
14干旱胁迫下葡萄AQP基因家族的鉴定及转录调控网络预测显示文摘【目的】探究干旱胁迫下葡萄AQP的转录调控网络,为后续研究葡萄抗旱基因功能和转录调控机制提供理论依据。【方法】通过生物信息学方法分析葡萄AQP基因家族成员的理化性质、系统进化和染色体定位,进行共线性分析和转录因子调控预测,通过转录组数据分析该基因家族在干旱胁迫和脱落酸(abscisic acid,ABA)处理下的表达情况。【结果】37个VvAQP基因主要分为PIP、TIP、NIP、SIP四类,分别分布在17条染色体上,复制分析结果表明,共有5对节段复制和2对串联复制。蛋白序列分析表明,每个VvAQP蛋白序列中均有1个植物AQP主要内在蛋白(major intrinsic proteins,MIP)的保守结构域特征。通过转录调控网络预测发现,靶向VvAQP基因的转录调控因子主要分为16类。干旱胁迫下,根和叶中大部分VvAQP基因表达持续下调,VvPIP2-3、VvPIP2-6在根中持续上调,7个VvAQP基因受ABA显著诱导。干旱胁迫下15个差异表达的转录因子可能与13个VvAQP基因存在调控关系。【结论】鉴定并提供了葡萄AQP基因家族成员的基本信息,通过转录组数据发现可能参与干旱胁迫的VvAQP基因,并预测了这些基因的转录调控网络。杨盛迪 郭大龙 裴茂松 刘海楠 韦同路 余义和 2021果树学报2021,38,10:3
15保卫细胞中ABA信号调控机制研究进展显示文摘脱落酸(ABA)具有调节植物快速响应逆境的重要功能。植物细胞中ABA核心信号通路由ABA受体PYR1/PYLs/RCARs、A类碱性蛋白磷酸酶PP2Cs和Snf1相关蛋白激酶SnRK2s组成。活性氧(ROS)和Ca^(2+)是保卫细胞中的重要第二信使,调控ABA诱导的气孔关闭。该文对保卫细胞中核心ABA信号蛋白的调控以及ROS和Ca^(2+)介导的ABA信号转导等最新研究成果进行综述,旨在阐明保卫细胞中ABA信号调控机制。周玉萍 颜嘉豪 田长恩 2022植物学报2022,57,5:2
16Molecular functions of nitric oxide and its potential applications in horticultural crops显示文摘Nitric oxide(NO)regulates plant growth,enhances nutrient uptake,and activates disease and stress tolerance mechanisms in most plants,making NO a potential tool for use in improving the yield and quality of horticultural crop species.Although the use of NO in horticulture is still in its infancy,research on NO in model plant species has provided an abundance of valuable information on horticultural crop species.Emerging evidence implies that the bioactivity of NO can occur through many potential mechanisms but occurs mainly through S-nitrosation,the covalent and reversible attachment of NO to cysteine thiol.In this context,NO signaling specifically affects crop development,immunity,and environmental interactions.Moreover,NO can act as a fumigant against a wide range of postharvest diseases and pests.However,for effective use of NO in horticulture,both understanding and exploring the biological significance and potential mechanisms of NO in horticultural crop species are critical.This review provides a picture of our current understanding of how NO is synthesized and transduced in plants,and particular attention is given to the significance of NO in breaking seed dormancy,balancing root growth and development,enhancing nutrient acquisition,mediating stress responses,and guaranteeing food safety for horticultural production.Chengliang Sun Yuxue Zhang Lijuan Liu Xiaoxia Liu Baohai Li Chongwei Jin Xianyong Lin 2021Horticulture Research2021,8,1:2
17乙烯对大豆幼苗盐胁迫响应的调控机制研究显示文摘为研究乙烯对大豆盐胁迫响应的调控作用,促进乙烯在大豆高效栽培中的应用,以耐盐大豆品种齐黄34为材料,采用水培的方法,外源添加乙烯合成前体1-氨基环丙烷-1-羧酸(1-aminocyclopropane-1-carboxylic acid,ACC)后进行NaCl胁迫处理,设置4种不同处理,测定盐处理对乙烯合成关键限速酶基因表达的影响,乙烯对盐胁迫下大豆幼苗形态建成、渗透调节系统和氧化还原系统的影响,分析乙烯对大豆抗盐的调控效应。结果显示:NaCl处理显著提高不同时间点乙烯合成关键限速酶基因GmACS1、GmACS2和GmACS6的表达,促进乙烯的释放;外源添加ACC可加速盐胁迫下大豆叶片衰老,降低地上部和地下部干物质和叶片叶绿素含量;提高植株地上部和地下部Na^(+)、K^(+)含量、地上部Na^(+)/K^(+)和MDA含量,降低脯氨酸含量;此外,外源添加ACC显著提高盐胁迫下大豆叶片O_(2)·-释放和NADPH氧化酶GmRbohA和GmRbohB基因的表达,降低POD酶活性。研究结果表明乙烯通过调控ROS稳态以及Na^(+)/K^(+)平衡负调控大豆耐盐性。王玉斌 刘薇 张彦威 李伟 王彩洁 戴海英 徐冉 张礼凤 2022大豆科学2022,41,5:2
18Arabidopsis U-box E3 ubiquitin ligase PUB11 negatively regulates drought tolerance by degrading the receptor-like protein kinases LRR1 and KIN7显示文摘Both plant receptor-like protein kinases(RLKs)and ubiquitin-mediated proteolysis play crucial roles in plant responses to drought stress.However,the mechanism by which E3 ubiquitin ligases modulate RLKs is poorly understood.In this study,we showed that Arabidopsis PLANT U-BOX PROTEIN 11(PUB11),an E3 ubiquitin ligase,negatively regulates abscisic acid(ABA)-mediated drought responses.PUB11 interacts with and ubiquitinates two receptor-like protein kinases,LEUCINE RICH REPEAT PROTEIN 1(LRR1)and KINASE 7(KIN7),and mediates their degradation during plant responses to drought stress in vitro and in vivo.pub11 mutants were more tolerant,whereas Irr1 and kin7 mutants were more sensitive,to drought stress than the wild type.Genetic analyses show that the pub11 Irr1 kin7 triple mutant exhibited similar drought sensitivity as the Irr1 kin7 double mutant,placing PUB11 upstream of the two RLKs.Abscisic acid and drought treatment promoted the accumulation of PUB11,which likely accelerates LRR1 and KIN7 degradation.Together,our results reveal that PUB11 negatively regulates plant responses to drought stress by destabilizing the LRR1 and KIN7 RLKs.Xuexue Chen Tingting Wang Amin Ur Rehman Yu Wang Junsheng Qi Zhen Li Chunpeng Song Baoshan Wang Shuhua Yang Zhizhong Gong 2021Journal of Integrative Plant Biology2021,63,3:2
19Transcriptomic and metabolomic analyses reveal that exogenous strigolactones alleviate the response of melon root to cadmium stress显示文摘Strigolactones(SLs)are classified into plant hormones,playing a key role as a mediator of plant growth in response to several abiotic stresses.Cadmium(Cd),a common heavy metal and soil pollutant,can suppress plant growth and development.In this work,we explored the effects of exogenous SLs on root formation in response to Cd stress using melon seeds subjected to seven germination treatments:CK(control),Cd(300 μmol·L^(-1) CdCl_(2)),and SL1-SL5(CdCl_(2)-stressed seeds pretreated with 0.1,0.5,1,2,and 3 μmol·L^(-1) GR24 solutions).The results indicated that SLs increased the antioxidant enzyme activities and root vigor and decreased the malondialdehyde(MDA)contents in the roots of Cdstressed melon seedlings.Then we used transcriptomic and metabolomic analyses to explore the mechanisms by which exogenous SLs protect against Cd stress.There were 242 significant differentially expressed genes(DEGs)(78 upregulated,164 downregulated)and 247 significantly differentially expressed metabolites(DEMs)(222 upregulated,25 downregulated)between the Cd and SL3 treatments.SLs altered the expression of genes related to redox formation processes,including peroxidase(POD),lipoxygenase(LOX),glutamate dehydrogenase(GDH),and glutathione S-transferase(GST).In addition,we found that SLs regulated the expression of the MYB,AP2/ERF,bHLH,and WRKY transcription factor families.The combined transcriptomic and metabolomic analyses revealed that the DEGs and DEMs involved in Cd stress alleviation were mainly related to the gene expression of jasmonic acid(JA)and flavonoid biosynthesis.SLs might induce LOX-related genes to regulate JA biosynthesis.Moreover,SLs might promote flavonoid biosynthesis by regulating eleven flavonoid-related genes and eight metabolites.The results provide a new perspective for studying the adaptation of plants to Cd stress.Xuemiao Chen Xueyin Shi Qing Ai Jinying Han Huaisong Wang Qiushi Fu 2022Horticultural Plant Journal2022,8,5:2
20参与辣椒素合成的DnaJ基因家族全表达谱分析显示文摘背景:DnaJ蛋白在植物发育和胁迫反应中发挥重要作用。近年来,通过对辣椒基因组全面的生信分析鉴定到76个DnaJ基因。但目前尚无辣椒DnaJ基因系统发育关系和表达谱的研究报道。因此,我们对不同组织、非生物胁迫和激素响应下辣椒DnaJ基因的系统发育关系和表达谱进行了系统的分析。结果:系统发育分析显示,辣椒DnaJ基因按序列同源性可分为7个亚族(Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅵ和Ⅶ亚族)。辣椒DnaJ基因在不同组织中的表达显示,38%(29/76)的辣椒DnaJ基因在至少一个组织中表达。结果表明,DnaJ基因在辣椒生长发育中具有潜在的关键作用。另外,为了解辛辣和非辛辣辣椒DnaJ基因在胎座中的表达差异,我们还利用RNA-Seq数据和qRT-PCR分析了辣椒DnaJ基因的表达模式。通过比较分析发现,8个基因在辛辣辣椒和非辛辣辣椒中的表达差异显著。CaDnaJs与胎座发育过程中参与辣椒素合成的基因共同表达。更重要的是,我们的研究揭示了这8个DnaJ基因可能受到胁迫(热、干旱和盐)的调控,同时也受到植物激素的调控(ABA,GA3,MeJA和SA)。结论:综上所述,部分在胎座中表达的DnaJ基因可能参与了植物在与刺激性相关化合物生物合成过程中对非生物胁迫的响应。本研究为辣椒DanJ基因的表达谱提供了广泛的认识,有助于我们对辣椒中的DnaJ基因功能认识。Fang Fanfei Liu Fawan Yang Xian Wan Hongjian Kang Yunyan 刘周斌(译) 2020辣椒杂志2020,,3:2
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