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| 1 | 超声提取葡萄籽原花青素工艺的优化及其抗氧化活性研究显示文摘研究利用响应曲面法优化超声提取葡萄籽原花青素的工艺。在单因素实验基础上,采用中心组合设计响应面实验,考察了提取温度、液料比、乙醇浓度以及超声时间对原花青素提取率的影响,并建立回归模型。优化后的工艺:提取温度55℃,液料比20(mL/g),乙醇浓度65%,超声时间10min;在此条件下葡萄籽原花青素的提取率为2.482%,与回归模型预测值的相对偏差为0.36%。同时进行了提取物的抗氧化性检验,结果表明葡萄籽原花青素对超氧阴离子自由基具有较好的清除能力,且呈剂量相关性。 | 金华 刘志刚 曾晓丹 高艳 马明硕 | 2014 | 中国调味品2014，39，4： | 21 |
| 2 | 应用GC-MS测定葡萄中的5种农药残留显示文摘 酿酒葡萄是河北昌黎的主要水果种植品种,由于受海洋气候的影响,当地空气湿热,葡萄病害十分严重,因而农药使用十分频繁,为了评价其农药残留水平,我们采用丙酮提取,凝胶分离,硅胶小柱净化,GC-MS的选择离子方式检测对采集的葡萄样品进行5种农药(百菌清、精甲霜灵、苯醚甲环唑、嘧菌酯、高效氯氟氰菊酯)的残留分析.…… | 秦曙 乔雄梧 王霞 周华 | 2004 | 分析测试学报2004，23，z1： | 14 |
| 3 | Inhibitory Effects of Grape Seed Proanthocyanidin Extract on Selenite-induced Cataract Formation and Possible Mechanism显示文摘This study investigated the inhibitory effect of grape seed proanthocyanidin extract(GSPE) on selenite-induced cataract formation in rats and the possible mechanism.Eighty 8-day-old Sprague-Dawley rats were divided randomly into 5 groups:control group,model group,three GSPE groups(low dose,medium dose and high dose).Control group received subcutaneous injection of physiological saline.Model group was given subcutaneous injection of sodium selenite(20 μmol/kg body weight) on the postpartum day 10,and once every other day for consecutive three times thereafter.GSPE treated groups were respectively administered GSPE at doses of 50,100,and 200 mg/kg body weight intragastrically 2 days prior to the selenite injection(that was,on the postpartum day 8),and once daily for fourteen consecutive days thereafter.The opacity of lenses was observed,graded and photographed under the slit lamp microscopy and the maximal diameter of the nuclear cataract plaques was measured.The lenses were analyzed for superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-PX),malondialdehyde(MDA),calcium(Ca 2+),nitric oxide(NO) and anti-hydroxyl radical ability(anti-OH).The histomorphology of lenses was observed with HE staining under a light microscope.The levels of calpainⅡ,and iNOS protein and mRNA expression in lenses were detected by using immunohistochemistry and real-time quantitative RT-PCR.The results showed subcutaneous injection of sodium selenite led to severe nuclear cataract in model group,and the achievement ratio of model group was 100%.As compared with model group,the degree of lenses opacity and the maximal diameter of nuclear cataract plaques were significantly reduced in GSPE-treated groups.Moreover,we observed selenite treatment caused a significant decrease in the activities of antioxidative enzymes(SOD,CAT,GSH-PX) and anti-OH ability,accompanied by a significant increase in the levels of MDA,NO,Ca 2+ as well as iNOS,and calpainⅡ protein and mRNA expression.Administration of GSPE could dose-dependently preserve the activities of these antioxidative enzymes and anti-OH ability,accompanied by a significant reduction in the levels of MDA,NO,Ca 2+ as well as iNOS,and calpainⅡ protein and mRNA expression.These results suggested that GSPE markedly prevented selenite-induced cataract formation probably by suppressing the generation of lipid peroxidation and free radicals as well as the activation of iNOS,and calpainⅡ in the lenses. | 张璇 胡义珍 | 2012 | Journal of Huazhong University of Science and Technology(Medical Sciences)2012，32，4： | 14 |
| 4 | Genome-wide Identification and Classification of HSF Family in Grape, and Their Transcriptional Analysis under Heat Acclimation and Heat Stress显示文摘Heat stress is one of the most serious threats in viticulture. Heat shock transcription factors(HSFs) have been proven to be the most important transcriptional factor family in the plant response to heat. In this study, a total of 19 putative HSF genes(VHSFs) were identified from the 12 × Vitis vinifera Pinot Noir PN4002 genome, and their chromosomal location, phylogenetic tree, exon-intron structure and conserved motifs were also analyzed. In addition, these HSFs transcriptional profiles in Vitis vinifera 'Jingxiu'(weak heat tolerance) and Vitis davidii 'Tangwei'(strong heat tolerance) under heat stress(47℃) and heat acclimation(38℃) were presented. Only VHSF01, VHSF05, VHSF15 and VHSF18 among 19 VHSFs showed different transcriptional models in these two genotypes under the same treatment, suggesting that these four VHSFs may be important factors which result in a thermotolerance difference between 'Jingxiu' and 'Tangwei'. This study provides novel insights into the response of 19 VHSFs to heat stress in the grape and suggests that potential key VHSFs in grape thermotolerance have great implications for molecular breeding in grapes. | LIU Guotian CHAI Fengmei WANG Yi JIANG Jinzhu DUAN Wei WANG Yuting WANG Fangfang LI Shaohua WANG Lijun | 2018 | Horticultural Plant Journal2018，4，4： | 13 |
| 5 | Red and Blue Lights Significantly Affect Photosynthetic Properties and Ultrastructure of Mesophyll Cells in Senescing Grape Leaves显示文摘Light quality significantly affects photosynthetic efficiency in plants. The mechanisms for how light quality affects photosynthesis in grape is poorly understood. Therefore, to investigate the effects of different light qualities on chloroplast ultrastructure and photosynthesis efficiency, two grape cultivars ‘Italia'(slower speed of leaf senescence) and ‘Centennial Seedless'(faster speed of leaf senescence) grown under protected and delayed conditions were used. The three treatments, replicated three times, were control(no supplemental lighting), red light and blue light. Chlorophyll content, net photosynthetic rate, and the ratio of F_v/F_m significantly increased in red light relative to the control. The opposite trend was observed in blue light in the early phase of leaf senescence. At later stages, physiological indexes were gradually higher than that of control, resulting in a delay in leaf senescence. Compared to the control, red and blue light both significantly increased the chlorophyll a/b ratio. Electron microscopy showed that blue light caused severe damage to the fine structure of chloroplasts at early stages of leaf senescence, but effects at later stages of leaf senescence became less severe compared to the control. The degradation of chloroplast ultrastructure was apparently delayed in red light throughout the experimental timeframe compared to other treatments. In this experiment, ‘Italia' showed higher chlorophyll content, net photosynthetic rate, ratios of F_v/F_m, chlorophyll a/b and better preserved chloroplast ultrastructure relative to ‘Centennial Seedless', resulting in a slower rate of leaf senescence. | WANG Shuai WANG Xiaodi SHI Xiangbin WANG Baoliang ZHENG Xiaocui WANG Haibo LIU Fengzhi | 2016 | Horticultural Plant Journal2016，2，2： | 10 |
| 6 | Current understanding of grapevine defense mechanisms against the biotrophic fungus (Erysiphe necator), the causal agent of powdery mildew disease显示文摘The most economically important disease of cultivated grapevines worldwide is powdery mildew(PM)caused by the ascomycete fungus Erysiphe necator.The majority of grapevine cultivars used for wine,table grape,and dried fruit production are derived from the Eurasian grape species Vitis vinifera because of its superior aroma and flavor characteristics.However,this species has little genetic resistance against E.necator meaning that grape production is highly dependent on the frequent use of fungicides.The integration of effective genetic resistance into cultivated grapevines would lead to significant financial and environmental benefits and represents a major challenge for viticultural industries and researchers worldwide.This review will outline the strategies being used to increase our understanding of the molecular basis of V.vinifera susceptibility to this fungal pathogen.It will summarize our current knowledge of different resistance loci/genes that have evolved in wild grapevine species to restrict PM infection and assess the potential application of these defense genes in the generation of PM-resistant grapevine germplasm.Finally,it addresses future research priorities which will be important in the rapid identification,evaluation,and deployment of new PM resistance genes which are capable of conferring effective and durable resistance in the vineyard. | Wenping Qiu Angela Feechan Ian Dry | 2015 | Horticulture Research2015，2，1： | 7 |
| 7 | Melatonin enhances salt tolerance by promoting MYB108A-mediated ethylene biosynthesis in grapevines显示文摘The signal molecules melatonin and ethylene play key roles in abiotic stress tolerance.The interplay between melatonin and ethylene in regulating salt tolerance and the underlying molecular mechanism of this interplay remain unclear.Here,we found that both melatonin and 1-aminocyclopropane-1-carboxylic acid(ACC,a precursor of ethylene)enhanced the tolerance of grapevine to NaCl;additionally,ethylene participated in melatonin-induced salt tolerance.Further experiments indicated that exogenous treatment and endogenous induction of melatonin increased the ACC content and ethylene production in grapevine and tobacco plants,respectively.The expression of MYB108A and ACS1,which function as a transcription factor and a key gene involved in ethylene production,respectively,was strongly induced by melatonin treatment.Additionally,MYB108A directly bound to the promoter of ACS1 and activated its transcription.MYB108A expression promoted ACC synthesis and ethylene production by activating ACS1 expression in response to melatonin treatment.The suppression of MYB108A expression partially limited the effect of melatonin on the induction of ethylene production and reduced melatonin-induced salt tolerance.Collectively,melatonin promotes ethylene biosynthesis and salt tolerance through the regulation of ACS1 by MYB108A. | Lili Xu Guangqing Xiang Qinghua Sun Yong Ni Zhongxin Jin Shiwei Gao Yuxin Yao | 2019 | Horticulture Research2019，6，1： | 7 |
| 8 | Comparison of phenolic profiles and antioxidant activities in skins and pulps of eleven grape cultivars(Vitis vinifera L.)显示文摘Eleven grape cultivars were analysed to explore the variety differences of fresh grape phenolic profiles. The results showed that free phenolics were predominant in grape skins and pulps, and showed the higher antioxidant activities than bound. In 11 cultivars, Muscat Kyoho extracts had the highest total phenolic content in skins(10.525 mg GAE g^(–1) FW) and pulps(1.134 mg GAE g^(–1) FW), and exhibited the highest DPPH radical scavening capacity(EC_(50)=11.7 μg mL^(–1)) and oxygen radical absorbance capacity(ORAC) value(190.57 μmol TE g^(–1) FW) of free phenolic in skin. In addition, the most abundant phenolics in grape skins were found to be flavonoids such as kaempferol in Kyoho skin(541.2 μg g^(–1) FW), rutin, catechin and epicatechin in Muscat Kyoho skin(262.3, 86.3 and 70.0 μg g^(–1) FW, respectively). Furthermore, the principal component analysis showed a strong difference of phenolic profiles with the cultivars, existing forms and distributions. Pearson correlation coefficient analysis showed a significant linear correlation between total phenolic content and antioxidant activity(P<0.05). Therefore, both skins and pulps were rich sources of bioactive phenolic compounds, and Muscat Kyoho was the ideal source among all samples. | LI Fu-xiang LI Fu-hua YANG Ya-xuan YIN Ran MING Jian | 2019 | Journal of Integrative Agriculture2019，18，5： | 7 |
| 9 | The grapevine kinome:annotation,classification and expression patterns in developmental processes and stress responses显示文摘Protein kinases(PKs)have evolved as the largest family of molecular switches that regulate protein activities associated with almost all essential cellular functions.Only a fraction of plant PKs,however,have been functionally characterized even in model plant species.In the present study,the entire grapevine kinome was identified and annotated using the most recent version of the grapevine genome.A total of 1168 PK-encoding genes were identified and classified into 20 groups and 121 families,with the RLK-Pelle group being the largest,with 872 members.The 1168 kinase genes were unevenly distributed over all 19 chromosomes,and both tandem and segmental duplications contributed to the expansion of the grapevine kinome,especially of the RLK-Pelle group.Ka/Ks values indicated that most of the tandem and segmental duplication events were under purifying selection.The grapevine kinome families exhibited different expression patterns during plant development and in response to various stress treatments,with many being coexpressed.The comprehensive annotation of grapevine kinase genes,their patterns of expression and coexpression,and the related information facilitate a more complete understanding of the roles of various grapevine kinases in growth and development,responses to abiotic stress,and evolutionary history. | Kaikai Zhu Xiaolong Wang Jinyi Liu Jun Tang Qunkang Cheng Jin-Gui Chen Zong-Ming(Max)Cheng | 2018 | Horticulture Research2018，5，1： | 5 |
| 10 | Melatonin Treatment Enhances the Polyphenol Content and Antioxidant Capacity of Red Wine显示文摘Melatonin and polyphenols are strong antioxidants, and melatonin is also a potential plant growth regulator. The role of melatonin in regulating polyphenol metabolism is unclear. This study assessed the primary impacts of exogenous melatonin treatment on phenolics accumulation and antioxidant capacity of the 'Moldova' wine. It was found that two times of 100 μmol·L^(-1) melatonin treatment clearly enhanced the endogenous melatonin content of ripened berries and wine. Further experiments indicated that melatonin treatment significantly increased the contents of total phenols, flavonoids and anthocyanins in wine. Additionally, the contents of most of the 20 detected individual phenolic compounds were significantly enhanced by melatonin treatment in wine. Particularly, the content of two non-flavonoid phenolic compounds(syringic and coumaric acid) and four anthocyanin compounds [Mv-3-Glu, Mv-3-(6-Coum)Glu-5-Glu, Dp-3-(6-AC)Glu and Dp-3-(6-Coum)Glu-5-Glu]were largely increased by melatonin treatment. In contrast, only Pn-3-(6-Coum)Glu was significantly reduced. Moreover, melatonin treatment significantly enhanced the antioxidant capacity of wine indicated by DPPH and FRAP assays. In summary, melatonin treatment enhanced the polyphenol content and antioxidant capacity of wine. | XU Lili YUE Qianyu BIAN Feng'e ZHAI Heng YAO Yuxin | 2018 | Horticultural Plant Journal2018，4，4： | 4 |
| 11 | The potential role of grape (Vitis vinifera L.) in prevention of threatened abortion via immunomodulatory and anti-inflammatory abilities: a hypothesis显示文摘Threatened abortion is a common problem in early pregnancy. This early vaginal bleeding happens in about 25% of pregnant women. The medications including progesterone, uterine muscle relaxant or human chorionic gonadotropin have essential effects in developing pregnancy, but the clinical data are insufficient to prescribe them. In recent decades, medicinal herbs can help us to present new treatments. Grape (Vitis vinifera L.) can protect the fetus from the perspective of Persian medicine. So, we hypothesize about the salutary effects of grape in miscarriage prevention. We found five standard expected mechanisms of grape to prevent threatened abortion: immunomodulatory and anti-inflammatory, anti-oxidant, anti-contraction, hormonal and anti-stress activities. Grape reduces nitric oxide, prostaglandin E2, expression of nuclear factor κB and other pro-inflammatory cytokines like IL (Interleukin)-1β, IL-6, IL-8, and tumor necrosis factor-α. It also elevates anti-inflammatory mediators and expression of peroxisome proliferator-activated receptor-γ. Grape polyphenols have a crucial role in fetus protection with high antioxidant power and other functions such as prevention of stress-triggered abortion with proanthocyanidins, or hormonal effects and inhibition of uterine contractions with resveratrol. So according to these studies, grape probably has effects on the immune and endocrine factors involved in threatened miscarriage. | Malihe Tabarrai Mozhgan Mehriardestani Sharareh Hekmat Fatemeh Nejatbakhsh Fatemeh Moradi | 2019 | Traditional Medicine Research2019，4，3： | 4 |
| 12 | Biochemical changes in grape rootstocks resulted from humic acid treatments in relation to nematode infection显示文摘Objective:To investigate the effect of humic acid on nematode infected,resistant and susceptible grapes in relation to lipid peroxidation and antioxidant mechanisms on selected biochemical parameters known as proactive substances.Methods:The grape rootstocks,superior,superior/ freedom and freedom were reacted differently to Meloidogyne incognita and Rotylenchulus reniformis according to rootstock progenitor.Two weeks after inoculation,two commercial products of humic acid were applied at the rate of(2,4 mL or grams/plant) as soil drench.After 4 months,nematode soil populations were extracted and counted.A subsample of roots from each plant was stained and gall numbers,embedded stages per root were calculated,final population, nematode build up(Pf/Pi),average of eggs/eggmass were estimated.Subsamples of fresh root of each treatment were chemically analyzed.Results:Freedom reduced significantly the nematode criteria and build up.Humic acid granules appeared to be more suppressive to nematode build up on superior and the higher dose on superior/freedom than liquid treatments.On freedom,all treatments reduced significantly the nematode build up regardless to the material nature.The higher dose was more effective than the lower one.As a result of humic acid applications,the malondialdehyde(MDA) and H_2O_2 contents were significandy reduced after humic acid treatments while the antioxidant compounds glutathione(GSH),ascorbic acid(ASA) and total phenol contents were significandy increased when compared with check.Antioxidant defense enzymes ascorbate peroxidase(AFX),superoxide dismutase(SOD),catalase(CAT) and polyphenol oxidase(PPO) showed significant increase in their specific activities in treated plants compared with nematode treated check.Conclnsions:Humic acid treatments improve we yield of grape by increasing the contents of antioxidant compounds and the specific activities of antioxidant enzymes. | Hosny H Kesba Hossam S El-Beltagi | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，4： | 4 |
| 13 | Effects of the severity and timing of basal leaf removal on the amino acids profiles of Sauvignon Blanc grapes and wines显示文摘The effects of the severity and timing of leaf removal(LR)on the amino acids of Sauvignon Blanc grapes and wines were studied during the 2017 growing season.High-performance liquid chromatography(HPLC)was used to analyze the amino acids profiles of grape berries and wines.The basal leaves were removed at three time points(40,56 and 72 days after flowering,named LR40,LR56 and LR72,respectively)at two severity levels(one at which the first,third,and fifth basal leaves of each shoot were removed(50%level);and another at which the first six basal leaves were removed(100%level)).The results showed that leaf removal had little impact on total soluble solids(°Brix),titratable acidity,pH or berry weight.The LR72-50%treated grapes had higher berry weight,titratable acidity and°Brix than those of the other treatments.The highest concentrations of total amino acids and of total amino acids except proline were detected in LR72-50%treated grapes(2 952.58 and 2 764.36 mg L^-1,respectively);the lowest were detected in LR72-100%treated grapes(2 172.82 and 2 038.71 mg L^-1,respectively).LR72-50%treatment significantly promoted the synthesis of aspartic acid,serine,arginine,alanine,aminobutyric acid and proline at both severity levels for grapes,the concentrations of all of these amino acids were increased relative to the control concentrations.The LR72-50%,LR40-100%and LR72-100%treated wines had higher total amino acids concentrations and higher concentrations of some individual amino acids,such as arginine,alanine and serine,than did the control wines.Of all the amino acids studied,glycine,tyrosine,cysteine,methionine and lysine were not significantly influenced by the timing or severity basal defoliation in grapes and wines.The present study reveals the effects of the timing and severity of leaf removal on the amino acids profiles of grapes and wines. | YUE Xiao-feng JU Yan-lun TANG Zi-zhu ZHAO Ya-meng JIAO Xu-liang ZHANG Zhen-wen | 2019 | Journal of Integrative Agriculture2019，18，9： | 3 |
| 14 | A key‘foxy’aroma gene is regulated by homologyinduced promoter indels in the iconic juice grape‘Concord’显示文摘‘Concord’,the most well-known juice grape with a parentage of the North American grape species Vitis labrusca L.,possesses a special‘foxy’aroma predominantly resulted from the accumulation of methyl anthranilate(MA)in berries.This aroma,however,is often perceived as an undesirable attribute by wine consumers and rarely noticeable in the common table and wine grape species V.vinifera.Here we discovered homology-induced promoter indels as a major genetic mechanism for species-specific regulation of a key‘foxy’aroma gene,anthraniloyl-CoA:methanol acyltransferase(AMAT),that is responsible for MA biosynthesis.We found the absence of a 426-bp and/or a 42-bp sequence in AMAT promoters highly associated with high levels of AMAT expression and MA accumulation in‘Concord’and other V.labrusca-derived grapes.These promoter variants,all with direct and inverted repeats,were further confirmed in more than 1,300 Vitis germplasm.Moreover,functional impact of these indels was validated in transgenic Arabidopsis.Superimposed on the promoter regulation,large structural changes including exonic insertion of a retrotransposon were present at the AMAT locus in some V.vinifera grapes.Elucidation of the AMAT genetic regulation advances our understanding of the‘foxy’aroma trait and makes it genetically trackable and amenable in grapevine breeding. | Yingzhen Yang JoséCuenca Nian Wang Zhenchang Liang Honghe Sun Benjamin Gutierrez Xiaojun Xi Jie Arro YiWang Peige Fan Jason Londo Peter Cousins Shaohua Li Zhangjun Fei Gan-Yuan Zhong | 2020 | Horticulture Research2020，7，1： | 3 |
| 15 | The genetic basis of grape and wine aroma显示文摘The grape is one of the oldest and most important horticultural crops.Grape and wine aroma has long been of cultural and scientific interest.The diverse compound classes comprising aroma result from multiple biosynthetic pathways.Only fairly recently have researchers begun to elucidate the genetic mechanisms behind the biosynthesis and metabolism of grape volatile compounds.This review summarizes current findings regarding the genetic bases of grape and wine aroma with an aim towards highlighting areas in need of further study.From the literature,we compiled a list of functionally characterized genes involved in berry aroma biosynthesis and present them with their corresponding annotation in the grape reference genome. | Jerry Lin Mélanie Massonnet Dario Cantu | 2019 | Horticulture Research2019，6，1： | 3 |
| 16 | 山东省葡萄白腐病病原菌鉴定显示文摘葡萄白腐病是世界范围内葡萄种植区的重要病害,每年可导致葡萄减产20%~30%[1-2],该病的病原菌有Coniella diplodiella (Speg.) Petrak & Sydow、C. fragariae (Oudem) B. Sutton、Pilidiella castaneicola和C. vitis[3-7]。1999年,LIU等[1]通过形态学研究明确了我国白腐病的病原为C. dip-lodiella。 | 袁丽芳 尹向田 蒋锡龙 李廷刚 魏彦锋 | 2023 | 植物病理学报2023，53，4： | 2 |
| 17 | Intellectual Property Protection and High-quality Development of Grape Industrial Clusters in Xinjiang显示文摘This paper introduced the grape industry in Xinjiang and its agricultural intellectual property resources,and analyzed six major problems such as the ineffective leading role of intellectual property rights in high-quality development.Finally,it proposed eight strategies to build a national-level regional fine-grain breeding base for grapes and develop“agricultural chips”in the context of the strategy of strengthening the country with intellectual property and innovation-driven development strategy. | Miaomiao XU Jinjin WANG Yulan BAI Jin ZENG Yuanpeng SUN Zhiguo SUN | 2023 | Asian Agricultural Research2023，15，5： | 2 |
| 18 | Effects of Temperature and pH on the Physiological Activity of Grape Juice显示文摘This study was conducted to investigate the effects of temperature and pH on polyphenol and flavonoid contents in grape juice and its antioxidant activity. Two groups(group A and group B) were set. Group A was set with different pH values: 3.0, 3.5, 4.0, 4.5 and 5.0; and group B was set with different temperatures: 65, 72, 79, 86 and 93 ℃. The grape juice was treated according to set methods, and two groups of samples were obtained. The contents of polyphenols and flavonoids in each test solution were determined, and the DPPH·free radical system and trivalent Fe reduction system were established to measure and compare the antioxidant activity of groups A and B. The results showed that within the set ranges, the effects of pH and temperature on the content of flavonoids in grape juice were not significant; the test solution with a pH of 4.5 and the test solution with a temperature at 72 ℃ had the strongest capacities of scavenging free radicals, and exhibited scavenging rates of 6.82% and 9.43%, respectively; the test solution with a pH of 4.5 and the test solution with a temperature at 65 ℃ had the strongest capacities of reducing ferric ions. It could be seen from the above four aspects comprehensively, within the set test ranges, the suitable pH was 4.5, and the suitable temperature was 65 ℃. | Danlei LI Chunling XIAO | 2019 | Agricultural Biotechnology2019，8，2： | 2 |
| 19 | N-doped honeycomb-like porous carbon towards high-performance supercapacitor显示文摘Biomass-derived porous carbon with developed pore structure is critical to achieving high performance electrode materials.In this work,we report a grape-based honeycomb-like porous carbon(GHPC)prepared by KOH activation and carbonization,followed by N-doping(NGHPC).The obtained NGHPC exhibits a unique honeycomb-like structure with hierarchically interconnected micro/mesopores,and high specific surface area of 1268 m^2/g.As a supercapacitor electrode,the NGPHC electrode exhibits a remarkable specific capacitance of 275 F/g at 0.5 A/g in a three-electrode cell.Moreover,the NGHPC//NGHPC symmetric supercapacitor displays a high energy density of 12.6 Wh/kg,and excellent cycling stability of approximately 95.2% capacitance retention after 5000 cycles at 5 A/g.The excellent electrochemical performance of NGHPC is ascribed to its high specific surface area,honeycomb-like structure and high-content of pyrodinic-N(36.29%).It is believed that grape-based carbon materials show great potential as advanced electrode materials for supercapacitors. | Feng Wang Lian Chen Huiling Li Gaigai Duan Shuijian He Lin Zhang Guoying Zhang Zhengping Zhou Shaohua Jiang | 2020 | Chinese Chemical Letters2020，31，7： | 2 |
| 20 | Differences of aroma development and metabolic pathway gene expression between Kyoho and 87-1 grapes显示文摘Aroma is an important quality trait of grapes and often the focus of consumers,viticulturists and grapevine breeders.Kyoho is a hybrid between Vitis vinifera and Vitis labrusca with a strawberry-like scent,while 87-1 is an early-ripening mutant of Muscat hamburg,belonging to Vitis vinifera,with a rose scent.In this study,we compared their aroma compositions and concentrations during berry development by headspace-SPME combined with gas chromatography-mass spectrometry(GC-MS),and analyzed the expression differences of enzyme-encoding genes in the LOX-HPL,MEP and MVA metabolic pathways by qRT-PCR.Twelve esters were detected in Kyoho during the whole berry development and they were abundant after veraison,but no esters were detected in 87-1 berries.Linalool was the dominant terpene among the 14 terpenes detected in 87-1 berries,while limited amounts of terpenes were detected in Kyoho berries.qRT-PCR analysis indicated that the low expression of VvAAT might explain the low content of ester volatiles in 87-1 berries,and the low expression of coding genes in the MEP pathway,especially VvPNLin Ner1,might be the reason for the low content of volatile terpenes in Kyoho berries.The results from this work will promote our understanding of aroma metabolic mechanisms of grapes,and offer some suggestions for grape aromatic quality improvement. | JI Xiao-hao W ANG Bao-liang WANG Xiao-di W ANG Xiao-long LIU Feng-zhi WANG Hai-bo | 2021 | Journal of Integrative Agriculture2021，20，6： | 2 |
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