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| 1 | Expression of IGF-Ⅱ,p53,p21 and HBxAg in precancerous events of hepatocarcinogenesis induced by AFBI and/or HBV in tree shrews显示文摘INTRODUCTIONIn order to study the relationship between oncogeneexpression and HCC generation,we observed theprecancerous hepatic GGT loci,IGF-Ⅱ,p53 andp21 expression during hepatocarcinogenesis of treeshrew induced by hepatitis B virus (HBV) and/oraflatoxin B1 (AFB1). | Qin LL Su JJ Li Y Yang C Ban KC Yian RQ | 2000 | World Journal of Gastroenterology2000，6，1： | 37 |
| 2 | The Ghd7 transcription factor represses ARE1 expression to enhance nitrogen utilization and grain yield in rice显示文摘The genetic improvement of nitrogen use efficiency(NUE)of crops is vital for grain productivity and sustainable agriculture.However,the regulatory mechanism of NUE remains largely elusive.Here,we report that the rice Grain number,plant height,and heading date7(Ghd7)gene genetically acts upstream of ABC1 REPRESSOR1(ARE1),a negative regulator of NUE,to positively regulate nitrogen utilization.As a transcriptional repressor,Ghd7 directly binds to two Evening Element-like motifs in the promoter and intron 1 of ARE1,likely in a cooperative manner,to repress its expression.Ghd7 and ARE1 display diurnal expression patterns in an inverse oscillation manner,mirroring a regulatory scheme based on these two loci.Analysis of a panel of 2656 rice varieties suggests that the elite alleles of Ghd7 and ARE1 have undergone diversifying selection during breeding.Moreover,the allelic distribution of Ghd7 and ARE1 is associated with the soil nitrogen deposition rate in East Asia and South Asia.Remarkably,the combination of the Ghd7 and ARE1 elite alleles substantially improves NUE and yield performance under nitrogen-limiting conditions.Collectively,these results define a Ghd7–ARE1-based regulatory mechanism of nitrogen utilization,providing useful targets for genetic improvement of rice NUE. | Qing Wang Qingmei Su Jinqiang Nian Jian Zhang Meng Guo Guojun Dong Jiang Hu Rongsheng Wang Changshuo Wei Guanwen Li Wan Wang Hui-Shan Guo Shaoyang Lin Wenfeng Qian XianzhiXie Qian Qian Fan Chen Jianru Zuo | 2021 | Molecular Plant2021，14，6： | 10 |
| 3 | cDNA-AFLP analysis reveals differential gene expression in incompatible interaction between infected non-heading Chinese cabbage and Hyaloperonospora parasitica显示文摘Non-heading Chinese cabbage(Brassica rapa ssp.chinensis)is one of the main green leafy vegetables in the world,especially in China,with significant economic value.Hyaloperonospora parasitica is a fungal pathogen responsible for causing downy mildew disease in Chinese cabbage,which greatly affects its production.The objective of this study was to identify transcriptionally regulated genes during incompatible interactions between non-heading Chinese cabbage and H.parasitica using complementary DNA-amplified fragment length polymorphism(cDNA-AFLP).We obtained 129 reliable differential transcript-derived fragments(TDFs)in a resistant line‘Suzhou Qing’.Among them,121 upregulated TDFs displayed an expression peak at 24–48 h post inoculation(h.p.i.).Fifteen genes were further selected for validation of cDNA-AFLP expression patterns using quantitative reverse transcription PCR.Results confirmed the altered expression patterns of 13 genes(86.7%)revealed by the cDNA-AFLP.We identified four TDFs related to fungal resistance among the 15 TDFs.Furthermore,comparative analysis of four TDFs between resistant line‘Suzhou Qing’and susceptible line‘Aijiao Huang’showed that transcript levels of TDF14(BcLIK1_A01)peaked at 48 h.p.i.and 25.1-fold increased in the resistant line compared with the susceptible line.Similarly,transcript levels of the other three genes,TDF42(BcCAT3_A07),TDF75(BcAAE3_A06)and TDF88(BcAMT2_A05)peaked at 24,48 and 24 h.p.i.with 25.1-,100-and 15.8-fold increases,respectively.The results suggested that the resistance genes tended to transcribe at higher levels in the resistance line than in the susceptible line,which may provide resistance against pathogen infections.The present study might facilitate elucidating the molecular basis of the infection process and identifying candidate genes for resistance improvement of susceptible cultivars. | Dong Xiao Shi-Tuo Liu Yan-Ping Wei Dao-Yun Zhou Xi-Lin Hou Ying Li Chun-Mei Hu | 2016 | Horticulture Research2016，3，1： | 8 |
| 4 | Gene diagnosis and targeted breeding for blast-resistant Kongyu 131without changing regional adaptability显示文摘The fungus Magnaporthe oryzae threatens the rice production of Kongyu 131 (KY131),a leading japonica variety in Northeast China.In this study,two rice lines,KP1 and KP2-Hd1,were obtained by introgressing the blast resistance genes Pi1 and Pi2 into KY131,respectively.However,both lines headed later than KY131.RICE60K SNP array analysis showed that Hd1 closely linked to Pi2 was introgressed into KP2-Hd1,and the linkage drag of Hd1 was broken by recombination.On the other hand,no known flowering genes were introgressed into KP1.Gene diagnosis by resequencing six flowering genes showed that KP1 carried functional Hd16 and Ghd8 alleles.Due to its suppression role in heading under long-day conditions,Ghd8 was chosen as the target for gene editing to disrupt its function.Four sgRNAs targeting different sites within Ghd8 were utilized to induce large-deletion mutations,which were easy to detect via agarose gel electrophoresis.All the ghd8-mutated KP1 lines were resistant to rice blast disease and headed earlier than the control KP1,even than KY131,under natural long-day conditions,which ensures its growth in Northeast China.This study confirmed that a combination of gene diagnosis and targeted gene editing is a highly efficient way to quickly eliminate undesired traits in a breeding line. | Xiangchun Zhou Gonghao Jiang Longwei Yang Lei Qiu Ping He Chunxiao Nong Yunyue Wang Yuqing He Yongzhong Xing | 2018 | Journal of Genetics and Genomics2018，45，10： | 7 |
| 5 | A chromosome-level reference genome of nonheading Chinese cabbage[Brassica campestris(syn.Brassica rapa)ssp.chinensis]显示文摘Non-heading Chinese cabbage(NHCC)is an important leafy vegetable cultivated worldwide.Here,we report the first high-quality,chromosome-level genome of NHCC001 based on PacBio,Hi-C,and Illumina sequencing data.The assembled NHCC001 genome is 405.33 Mb in size with a contig N50 of 2.83 Mb and a scaffold N50 of 38.13 Mb.Approximately 53%of the assembled genome is composed of repetitive sequences,among which long terminal repeats(LTRs,20.42%of the genome)are the most abundant.Using Hi-C data,97.9%(396.83 Mb)of the sequences were assigned to 10 pseudochromosomes.Genome assessment showed that this B.rapa NHCC001 genome assembly is of better quality than other currently available B.rapa assemblies and that it contains 48,158 protein-coding genes,99.56%of which are annotated in at least one functional database.Comparative genomic analysis confirmed that B.rapa NHCC001 underwent a whole-genome triplication(WGT)event shared with other Brassica species that occurred after the WGD events shared with Arabidopsis.Genes related to ascorbic acid metabolism showed little variation among the three B.rapa subspecies.The numbers of genes involved in glucosinolate biosynthesis and catabolism were higher in NHCC001 than in Chiifu and Z1,due primarily to tandem duplication.The newly assembled genome will provide an important resource for research on B.rapa,especially B.rapa ssp.chinensis. | Ying Li Gao-Feng Liu Li-Ming Ma Tong-Kun Liu Chang-Wei Zhang Dong Xiao Hong-Kun Zheng Fei Chen Xi-Lin Hou | 2020 | Horticulture Research2020，7，1： | 6 |
| 6 | Hydrogen sulfide promotes flowering in heading Chinese cabbage by S-sulfhydration of BraFLCs显示文摘Heading Chinese cabbage(Brassica rapa L.syn.B.campestris L.ssp.chinensis Makino var.pekinensis(Rupr.)J.Cao et Sh.Cao)is a cruciferous Brassica vegetable that has a triplicate genome,owing to an ancient genome duplication event.It is unclear whether the duplicated homologs have conserved or diversi fied functions.Hydrogen sulfide(H_(2)S)is a plant gasotransmitter that plays important physiological roles in growth,development,and responses to environmental stresses.The modification of cysteines through S-sulfhydration is an important mechanism of H_(2)S,which regulates protein functions.H?S promotes flowering in Arabidopsis and heading Chinese cabbage.Here we investigated the molecular mechanisms of H_(2)S used to promote flowering in the latter.Four,five,and four BraFLC,BraSOC I,and BraFT homologs were identi fi ed in heading Chinese cabbage.Different BraFLC proteins were bound to different CArG boxes in the promoter regions of the BraSOC I and BraFT homologs,producing different binding patterns.Thus,there may be functionally diverse BraFLC homologs in heading Chinese cabbage.Exogenous H_(2)S at 100μmol L^(-1) significantly promoted flowering by compensating for insuf fi cient vernalization.BraFLC 1 and BraFLC_(3) underwent S-sulfhydration by H_(2)S,after which their abilities to bind most BraSOC I or BraFT promoter probes weakened or even disappeared.These changes in binding ability were consistent with the expression pattern of the BraFT and BraSOC I homologs in seedlings treated with H_(2)S.These results indicated that H_(2)S signaling regulates flowering time.In summary,H_(2)S signaling promoted plant flowering by weakening or eliminating the binding abilities of BraFLCs to downstream promoters through S-sulfhydration. | Xiaoli Ma Liping Zhang Zhuoya Pei Linlin Zhang Zhiqiang Liu Danmei Liu Xuefeng Hao Zhuping Jin Yanxi Pei | 2021 | Horticulture Research2021，8，1： | 6 |
| 7 | Author Correction: Skull optical clearing window for in vivo imaging of the mouse cortex at synaptic resolution显示文摘Correction to:Light:Science&Applications(2018)7,17153;https://doi.org/10.1038/lsa.2017.153;Article published online,23 February 2018.In the version of this article originally published,in the“Materials and methods”section under subheading“Reagents”,there are errors about the information of reagents.And the original and corrected versions are shown below. | Yan-Jie Zhao Ting-Ting Yu Chao Zhang Zhao Li Qing-Ming Luo Tong-Hui Xu Dan Zhu | 2018 | Light(Science & Applications)2018，7，1： | 6 |
| 8 | Exploring Seasonal and Circadian Rhythms in Structural Traits of Field Maize from LiDAR Time Series显示文摘Plant growth rhythm in structural traits is important for better understanding plant response to the ever-changing environment.Terrestrial laser scanning(TLS)is a well-suited tool to study structural rhythm under field conditions.Recent studies have used TLS to describe the structural rhythm of trees,but no consistent patterns have been drawn.Meanwhile,whether TLS can capture structural rhythm in crops is unclear.Here,we aim to explore the seasonal and circadian rhythms in maize structural traits at both the plant and leaf levels from time-series TLS.The seasonal rhythm was studied using TLS data collected at four key growth periods,including jointing,bell-mouthed,heading,and maturity periods.Circadian rhythms were explored by using TLS data acquired around every 2 hours in a whole day under standard and cold stress conditions.Results showed that TLS can quantify the seasonal and circadian rhythm in structural traits at both plant and leaf levels.(1)Leaf inclination angle decreased significantly between the jointing stage and bell-mouthed stage.Leaf azimuth was stable after the jointing stage.(2)Some individual-level structural rhythms(e.g.,azimuth and projected leaf area/PLA)were consistent with leaf-level structural rhythms.(3)The circadian rhythms of some traits(e.g.,PLA)were not consistent under standard and cold stress conditions.(4)Environmental factors showed better correlations with leaf traits under cold stress than standard conditions.Temperature was the most important factor that significantly correlated with all leaf traits except leaf azimuth.This study highlights the potential of time-series TLS in studying outdoor agricultural chronobiology. | Shichao Jin Yanjun Su Yongguang Zhang Shilin Song Qing Li Zhonghua Liu Qin Ma Yan Ge LingLi Liu Yanfeng Ding Frédéric Baret Qinghua Guo | 2021 | Plant Phenomics2021，3，1： | 5 |
| 9 | A PRELIMINARY REPORT ON MALE STERILITY IN RICE, Oryza sativa L.显示文摘Through panicle to panicle inspection at heading stage, six male-sterile rice plants were found in the paddy fields where several varieties were grown from 1964 to 1965 in Hunan Province. The frequency of occurrence of such male-sterile rice plants under natural conditions was estimated to be 0.13 per cent. According to the characteristics of their anthers and pollen grains, these male-sterile plants can be distinguished into three different types: (1) Pollen-free type. Two plants of this type were found to be completely malesterile. Their anthers are smaller and thinner than those of the normal plant as shown in Fig. 1. They are colourless, remain unbroken after flowering, and contain no pollen grains. | 袁隆平 | 1966 | Chinese Science Bulletin1966，24，7： | 5 |
| 10 | Genome-wide transcriptome analysis reveals molecular pathways involved in leafy head formation of Chinese cabbage(Brassica rapa)显示文摘Chinese cabbage plants go through seedling and rosette stages before forming their leafy head.Chinese cabbage plants resemble pak-choi plants at their seedling stage,but in their rosette stage the leaves of Chinese cabbage differentiate,as they increase in size with shorter petioles.In order to understand the molecular pathways that play a role in leafy head formation,transcript abundance of young emerging leaves was profiled during development of two Chinese cabbage genotypes and a single pak-choi genotype.The two Chinese cabbages differed in many aspects,among others earliness,leaf size and shape,leaf numbers,and leafy head shape.Genome-wide transcriptome analysis clearly separated the seedling stages of all three genotypes together with the later stages from pak-choi,from the later developmental stages of both Chinese cabbages(rosette,folding,and heading).Weighted correlation network analysis and hierarchical clustering using Euclidean distances resulted in gene clusters with transcript abundance patterns distinguishing the two Chinese cabbages from pak-choi.Three clusters included genes with transcript abundance affected by both genotype and developmental stage,whereas two clusters showed only genotype effects.This included a genotype by developmental stage cluster highly enriched with the MapMan category photosynthesis,with high expression during rosette and folding in Chinese cabbages and low expression in the heading inner leaves that are not exposed to light.The other clusters contained many genes in the MapMan categories Cell,showing again differences between pak-choi and both Chinese cabbages.We discuss how this relates to the differences in leaf blade growth between Chinese cabbage and pak-choi,especially at the rosette stage.Overall,comparison of the transcriptome between leaves of two very different Chinese cabbages with pak-choi during plant development allowed the identification of specific gene categories associated with leafy head formation. | XiaoXue Sun Ram Kumar Basnet Zhichun Yan Johan Bucher Chengcheng Cai Jianjun Zhao Guusje Bonnema | 2019 | Horticulture Research2019，6，1： | 4 |
| 11 | Genetic Interaction of Hd1 with Ghd7, DTH8 and Hd2 Largely Determines Eco-Geographical Adaption of Rice Varieties in Southern China显示文摘Although cultivated rice originated from the tropical region,a long process of domestication and human selection has enabled cultivated rice to grow in a wide range of geographical regions.Diversification of photoperiodic flowering provides a foundation for this diverse adaptation.Intensive studies have focused on elucidating how japonica varieties lost photoperiodic sensitivity(PS)to expand their cultivation areas to high latitudes,where rice is grown in a short season when day-length is long(Fujino et al,2013;Naranjo et al,2014;Gomez-Ariza et al,2015;Li et al,2015,2018;Goretti et al,2017;Ye et al,2018).By contrast,limited attention has been paid to the genetic architecture of heading date(HD)variation among varieties in middle and low latitudes.The southern China rice region,located in middle and low latitudes,occupies the most important rice cultivation region in China.This region is predominantly planted with indica varieties,which presents a rich diversity of regional and seasonal adaptations.In the present study,improved varieties and landraces used in this region were analyzed for allelic variations of 12 cloned QTLs controlling HD,as well as for the genotypic effects of these genes on HD and PS.Our objective was to clarify predominant genetic factors influencing ecogeographical adaption of rice varieties in southern China by comparing improved varieties with landraces. | ZHANG Zhenhua ZHU Yujun WANG Shilin FAN Yeyang ZHUANG Jieyun | 2021 | Rice science2021，28，2： | 2 |
| 12 | An efficient screening system to identify protein-protein or protein-DNA interaction partners of rice transcription factors显示文摘Plant transcription factors(TFs)are key molecular components in the regulation of plant growth,development,and environmental adaptability.Generally,TFs need to form complexes with other interactors to control gene transcription.For example,Heading date 1(Hd1),Grain number,plant height and heading-date(Ghd)7,or Ghd7.1 interacts with Ghd8 and NF-YCs to form a trimeric complex,respectively. | Qingmei Su Fang Zhang Yunping Xiao Pingping Zhang Hefei Xing Fan Chen | 2022 | Journal of Genetics and Genomics2022，49，10： | 1 |
| 13 | OPTIMIZATION OF MATERIAL PROPERTIES THROUGH THE LINKAGE OF SIMULATION CALCULATIONS WITH THE REAL ROLLING PROCESS OF DIFFERENT STEEL GRADES FOR BABS AND WIRE ROD显示文摘Material properties were optimized through the linkage of numericalsimulation with the real rolling pro- cess. A few different steel grades for bars and wire rode were taken as the examples.The examples.The commerical FEM code MARC/MENTAT was used for the numerical simulation of the three - dimensional elas- toplastic rolling process coupled with temperature.The simulation calculation for temperation for temperature control in- side the rolling mill was carried out by use of the SMS - developed CCT system (controlled coling technolgy). New rolling - technological precess was developed for bars and wire rods based on the numerical results.Steel 38MnSiVS5 and steel 41Cr4 were taken as the examples. Keywrods: rolling,numerical simulation, material property | R. Kohlmann M. Kruse M. Meyer and U. Plociennik( 1) Betriebsleiter Qualitatsstelle, Krupp Edelstahl Profile, Siegen 2)Research and Development, SMS Schloemann Siemap AG, Dusseldorf/Germany) | 2000 | Acta Metallurgica Sinica(English Letters)2000，13，1： | 1 |
| 14 | Genome-wide analysis of changes in miRNA and target gene expression reveals key roles in heterosis for chinese cabbage biomass显示文摘Heterosis is a complex phenomenon in which hybrids show better phenotypic characteristics than their parents do.Chinese cabbage(Brassica rapa L.spp.pekinensis)is a popular leafy crop species,hybrids of which are widely used in commercial production;however,the molecular basis of heterosis for biomass of Chinese cabbage is poorly understood.We characterized heterosis in a Chinese cabbage hybrid cultivar and its parental lines from the seedling stage to the heading stage;marked heterosis of leaf weight and biomass yield were observed.Small RNA sequencing revealed 63 and 50 differentially expressed microRNAs(DEMs)at the seedling and early-heading stages,respectively.The expression levels ofthe majority of miRNA clusters in the hybrid were lower than the mid-parent values(MPVs).Using degradome sequencing,we identi fied 1,819 miRNA target genes.Gene ontology(GO)analyses demonstrated that the target genes ofthe MPV-DEMs and low parental expression level dominance(ELD)miRNAs were signi ficantly enriched in leaf morphogenesis,leaf development,and leaf shaping.Transcriptome analysis revealed that the expression levels of photosynthesis and chlorophyll synthesis-related MPV-DEGs(differentially expressed genes)were signi ficantly different in the F_(1) hybrid compared to the parental lines,resulting in increased photosynthesis capacity and chlorophyll content in the former.Furthermore,expression of genes known to regulate leaf development was also observed at the seedling stage.Arabidopsis plants overexpressing BrGRF4.2 and bra-miR396 presented increased and decreased leaf sizes,respectively.These results provide new insight into the regulation of target genes and miRNA expression patterns in leaf size and heterosis for biomass of B.rapa. | Peirong Li Tongbing Su Deshuang Zhang Weihong Wang Xiaoyun Xin Yangjun Yu Xiuyun Zhao Shuancang Yu Fenglan Zhang | 2021 | Horticulture Research2021，8，1： | 1 |
| 15 | 杂种小麦繁种制种技术初步研究显示文摘繁种制种技术研究是提高繁种制种产量,促进杂种小麦走向大面积生产的关键研究内容之一。与制种产量有关的因素很多,其中包括行比、花期相遇与否。 | 张爱民 黄铁城 阿以提古丽 | 1993 | 中国农业大学学报1993，，S2： | 1 |
| 16 | 小麦抽穗期基因研究进展显示文摘抽穗期是小麦品种的重要农艺性状之一,它对于小麦适应不同生态地区和不同环境条件具有至关重要的作用。春化反应、光周期反应和早熟性基因是影响小麦抽穗期的3种重要因素。综述了小麦抽穗期相关基因的定位和克隆,分析了控制小麦抽穗的3类基因性状以便为研究小麦的抽穗期基因提供参考。 | 董春林 张明义 张晓军 | 2010 | 江西农业学报2010，22，9： | 1 |
| 17 | Genotypic Analysis of Heading Time on an Indica Rice Cultivar,Nanjing 11显示文摘In this paper, the genotype of heading time in Nanjing 11, an indica variety, was analyzed bycrossing with tester varieties, Akihikari ( e1e1 e2e2 e3e3 Se1eSe1e ), Koshihikari ( E1E1E2E2 e3e3 Se1eSe1e ), Nipponbare(E1E1 e2e2 e3e3 Se1Se1 )and Hinohikari(E1E1 E2E2 e3e3 Se1Se1 ), of which the genotypes of headingtime were well known. The results showed genotype of heading time in Nanjing 11 was E1E1 e2e2 E3E3 Se1Se1and also included a recessive inhibitor I-Se1 for photoperiod-sensitivity as well. Meanwhile, the two photoperiod-sensitive genes, E1 and Se1, in Nanjing 11 were also identified by crossing with QTL nearly isogenic lines of Nipponbare, NIL( Hd1 )and NIL( Hd4 )which have complementary effects. | LUO Lin-guang, SU Chang-chao, Eiji Shimura, ZHAI Hu-qu and WAN Jian-min( State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095 ) | 2002 | Agricultural Sciences in China2002，1，1： | 1 |
| 18 | 缤纷前沿(英文)显示文摘J.K.Rowling Has Written a New Book Under the Name Robert Galbraith罗琳匿名出新书J.K.Rowling has published a crime novel called The Cuckoo’s Calling under the pseudonym(笔名) Robert Galbraith.After she was exposed as the authoress in July,Rowling said,'I had hoped to keep this secret | 夏之黎 | 2013 | 新东方英语(中学版)2013，0，9： | 0 |
| 19 | Opportunity in Adversity显示文摘The ongoing novel coronavirus epidemic seemed to have pressed a pause button to the movement of most people over the past few weeks.According to Cong Liang,Secretary General of the National Development and Reform Commission(NDRC),apart from Hubei Province,the hardest-hit place,other provincial-level regions are heading back to work. | Zhang Shasha | 2020 | ChinAfrica2020，12，3： | 0 |
| 20 | PEOPLE & POINTS显示文摘NEW SPORT ADMINISTRATOR Gao Zhidan has been promoted to head the General Administration of Sport of China(GASC).Born in Siping, Jilin Province, in 1963, Gao graduated from the Beijing Sport University at the age of 25. He has spent his career at the GASC, including heading the department in charge of the country’s shooting and archery disciplines. | | 2022 | Beijing Review2022，65，32： | 0 |
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