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| 1 | A Genomic Variation Map Provides Insights into the Genetic Basis of Spring Chinese Cabbage (Brassica rapa ssp.pekinensis)Selection显示文摘Chinese cabbage is the most consumed leafy crop in East Asian countries.However,premature bolting induced by continuous low temperatures severely decreases the yield and quality of the Chinese cabbage, and therefore restricts its planting season and geographic distribution.In the past 40years,spring Chinese cabbage with strong winterness has been selected to meet the market demand.Here,we report a genome variation map of Chinese cabbage generated from the resequencing data of 194 geographically diverse accessions of three ecotypes.In-depth analyses of the selection sweeps and genome-wide patterns revealed that spring Chinese cabbage was selected from a specific population of autumn Chinese cabbage around the area of Shandong peninsula in northern China.We identified 23 genomic loci that underwent intensive selection,and further demonstrated by gene expression and haplotype analyses that the incorporation of elite alleles of VERNALISATION INSENTIVE 3.1(BrVIN3.1)and FLOWER LOCUS C 1(BrFLC1)is a determinant genetic source of variation during selection.Moreover,we showed that the quantitative response of BrVIN3.1 to cold due to the sequence variations in the cis elements of the BrVlN3.1 promoter significantly contributes to bolting-time variation in Chinese cabbage.Collectively, our study provides valuable insights into the genetic basis of spring Chinese cabbage selection and will facilitate the breeding of bolting-resistant Varieties by molecular-marker-assisted selection,transgenic or gene editingapproaches. | Tongbing Su Weihong Wang Peirong Li Bin Zhang Pan Li Xiaoyun Xin Honghe Sun Yangjun Yu Deshuang Zhang Xiuyun Zhao Changlong Wen Gang Zhou Yuntong Wang Hongkun Zheng Shuancang Yu Fenglan Zhang | 2018 | Molecular Plant2018，11，11： | 6 |
| 2 | Cloning and expression of the Chinese wheat mosaic virus RNA2 coat protein read-through and 19 ku cysteine-rich domains and localization of these proteins显示文摘The 5’-terminal (RTn) and 3’-terminal (RTc) halves of the coat protein readthrough domain and the 19 ku cysteine-rich protein of Chinese wheat mosaic virus (CWMV) were amplified by RT-PCR, cloned and expressed in E. coll. Antisera and monoclonal antibodies against these proteins were prepared by immunising these purified proteins to mice. Detection of RTn, RTc and 19 ku proteins in CWMV infected wheat sap and leaf tissue indicated that the RTn and RTc proteins were distributed on the surface of virus particles whereas the 19 ku protein was in the cytoplasm of the infected wheat cells. | XU Lei CHEN Jianping YE Rong ZHAO Xiuyun YU Shanqing | 2002 | Chinese Science Bulletin2002，47，12： | 4 |
| 3 | Identification of long noncoding RNAs involved in resistance to downy mildew in Chinese cabbage显示文摘Brassica downy mildew,a severe disease caused by Hyaloperonospora brassicae,can cause enormous economic losses in Chinese cabbage(Brassica rapa L.ssp.pekinensis)production.Although some research has been reported recently concerning the underlying resistance to this disease,no studies have identified or characterized long noncoding RNAs involved in this defense response.In this study,using high-throughput RNA sequencing,we analyzed the disease-responding mRNAs and long noncoding RNAs in two resistant lines(T12–19 and 12–85)and one susceptible line(91–112).Clustering and Gene Ontology analysis of differentially expressed genes(DEGs)showed that more DEGs were involved in the defense response in the two resistant lines than in the susceptible line.Different expression patterns and proposed functions of differentially expressed long noncoding RNAs among T12–19,12–85,and 91–112 indicated that each has a distinct disease response mechanism.There were significantly more cis-and trans-functional long noncoding RNAs in the resistant lines than in the susceptible line,and the genes regulated by these RNAs mostly participated in the disease defense response.Furthermore,we identified a candidate resistance-related long noncoding RNA,MSTRG.19915,which is a long noncoding natural antisense transcript of a MAPK gene,BrMAPK15.Via an agroinfiltration-mediated transient overexpression system and virus-induced gene silencing technology,BrMAPK15 was indicated to have a greater ability to defend against pathogens.MSTRG.19915-silenced seedlings showed enhanced resistance to downy mildew,probably because of the upregulated expression of BrMAPK15.This research identified and characterized long noncoding RNAs involved in resistance to downy mildew,laying a foundation for future in-depth studies of disease resistance mechanisms in Chinese cabbage. | Bin Zhang Tongbing Su Peirong Li Xiaoyun Xin Yunyun Cao Weihong Wang Xiuyun Zhao Deshuang Zhang Yangjun Yu Dayong Li Shuancang Yu Fenglan Zhang | 2021 | Horticulture Research2021，8，1： | 3 |
| 4 | rAdinbitor, a novel disintegrin from Agkistrodon halys brevicaudus stejneger inhibits adhesion and proliferation of SMMC-7721 cells显示文摘Objective: To investigate the effects of rAdinbitor on the adhesion and proliferation of human hepatoma cell strain SMMC-7721. Methods: Cell adhesion assay was used to observe the effect of rAdinbitor on the adhesion of SMMC-7721 cells to fibronectin (FN). Crystal violet staining was performed to detect the influence of rAdinbitor on the adhesion of SMMC-7721 cells. MTT assay was employed to detect the inhibitory effects of different concentration of rAdinbitor on the proliferation of SMMC-7721 cells. The morphologic changes of the control SMMC-7721 cells and the apoptotic cells induced by 200 μg/mL rAdinbitor for 36 h were observed under light microscope after HE staining. Flow cytometry analysis was applied to determine the apoptosis rate of SMMC-7721 cells. Results: (1) FN promoted the adhesion of human hepatoma cell strain SMMC-7721 in a dose-dependent manner. (2) rAdinbitor could dose-dependently inhibit the adhesion of SMMC-7721 cells to FN. The higher the concentration was, the stronger the inhibition was. There was significant difference among the groups (P < 0.05). (3) rAdinbitor had a strong inhibition on the proliferation of SMMC-7721 cells and showed a dose-dependent manner (P < 0.05). After a 48 h exposure, the IC50 value of rAdinbitor was 177.83 μg/mL. (4) After exposure of SMMC-7721 cells to 200 μg/mL rAdinbitor for 36 h, the early morphologic changes appeared and the apoptosis rate was 20.68%, significantly higher than that of the control group (2.38%, P < 0.05). Conclusion: rAdinbitor can dose-dependently inhibit the SMMC-7721 cells adhesion to FN, and can inhibit the proliferation in dose-dependent manner and promote their apoptosis. | Chunling Zhao Xiuyun Cui Feng Ren Baochang Zhao | 2008 | The Chinese-German Journal of Clinical Oncology2008，7，7： | 2 |
| 5 | Molecular dynamics simulation of graphene sinking during chemical vapor deposition growth on semi-molten Cu substrate显示文摘Copper foil is the most promising catalyst for the synthesis of large-area,high-quality monolayer graphene.Experimentally,it has been found that the Cu substrate is semi-molten at graphene growth temperatures.In this study,based on a self-developed C–Cu empirical potential and density functional theory(DFT)methods,we performed systematic molecular dynamics simulations to explore the stability of graphene nanostructures,i.e.,carbon nanoclusters and graphene nanoribbons,on semi-molten Cu substrates. | Ziwei Xu Guanghui Zhao Lu Qiu Xiuyun Zhang Guanjun Qiao Feng Ding | 2020 | npj Computational Materials2020，，1： | 2 |
| 6 | Expression Profiles of Six Circulating MicroRNAs Critical to Atherosclerosis in Patients With Subclinical Hypothyroidism: A Clinical Study显示文摘 | Xinhuan Zhang Shanshan Shao Houfa Geng Yong Yu Chenggang Wang Zhanfeng Liu Chunxiao Yu Xiuyun Jiang Yangxin Deng Ling Gao Jiajun Zhao | 2014 | The Journal of Clinical Endocrinology & Metabolism2014，，： | 1 |
| 7 | Genome-wide analysis of changes in miRNA and target gene expression reveals key roles in heterosis for chinese cabbage biomass显示文摘Heterosis is a complex phenomenon in which hybrids show better phenotypic characteristics than their parents do.Chinese cabbage(Brassica rapa L.spp.pekinensis)is a popular leafy crop species,hybrids of which are widely used in commercial production;however,the molecular basis of heterosis for biomass of Chinese cabbage is poorly understood.We characterized heterosis in a Chinese cabbage hybrid cultivar and its parental lines from the seedling stage to the heading stage;marked heterosis of leaf weight and biomass yield were observed.Small RNA sequencing revealed 63 and 50 differentially expressed microRNAs(DEMs)at the seedling and early-heading stages,respectively.The expression levels ofthe majority of miRNA clusters in the hybrid were lower than the mid-parent values(MPVs).Using degradome sequencing,we identi fied 1,819 miRNA target genes.Gene ontology(GO)analyses demonstrated that the target genes ofthe MPV-DEMs and low parental expression level dominance(ELD)miRNAs were signi ficantly enriched in leaf morphogenesis,leaf development,and leaf shaping.Transcriptome analysis revealed that the expression levels of photosynthesis and chlorophyll synthesis-related MPV-DEGs(differentially expressed genes)were signi ficantly different in the F_(1) hybrid compared to the parental lines,resulting in increased photosynthesis capacity and chlorophyll content in the former.Furthermore,expression of genes known to regulate leaf development was also observed at the seedling stage.Arabidopsis plants overexpressing BrGRF4.2 and bra-miR396 presented increased and decreased leaf sizes,respectively.These results provide new insight into the regulation of target genes and miRNA expression patterns in leaf size and heterosis for biomass of B.rapa. | Peirong Li Tongbing Su Deshuang Zhang Weihong Wang Xiaoyun Xin Yangjun Yu Xiuyun Zhao Shuancang Yu Fenglan Zhang | 2021 | Horticulture Research2021，8，1： | 1 |
| 8 | Effect of Low Temperature on Germination Characteristics of Rice Cultivars显示文摘The effects of low temperature on germination characteristics of rice seeds were studied,using three rice varieties as materials.The results showed that the germination rate,germination energy,germination index,vigor index,shoot length and root length all reduced with the decrease of temperature.When the treatment temperature was 10 ℃,whether or not cold-resistant varieties,the values of all parameters showed an extremely significant difference from the control's.When the stress temperature was 15 ℃,compared with the normal condition,the parameter values of the non-tolerant variety( Changbai 9) had an extremely significant difference and the germination rate,germination potential,seedling length and root length of two cold-tolerance varieties( Jisheng 202 and Tongjing 9) had little difference from the control.Therefore,the above four indicators at 15 ℃ can be considered as the evaluation indices of cold tolerance at early stage. | Yadong ZHAO Dianfa ZHOU Xiuyun LIN Qiang SUN Pengzhi Li Ding Zaisong Jinming WANG | 2018 | Agricultural Biotechnology2018，7，6： | 1 |
| 9 | Perceived HIV Stigma among Children in a High HIV-prevalenee Area in Central China: Beyond the Parental HIV-related Illness and Death显示文摘 | Xiuyun Lin Guoxiang Zhao Xiaoming Li | 2010 | AIDS Care2010，22，4： | 1 |
| 10 | Microstructure and mechanical properties of a large billet of spray formed Al–Zn–Mg–Cu alloy with high Zn content显示文摘 | Pucun Bai Xiaohu Hou Xiuyun Zhang Chunwang Zhao Yongming Xing | 2009 | Materials Science & Engineering A2009，，1： | 1 |
| 11 | High-performance self-organized Si nanocomposite anode for lithium-ion batteries显示文摘Silicon is being investigated extensively as an anodic material for next-generation lithium ion batteries for portable energy storage and electric vehicles.However,the large changes in volume during cycling lead to the breakdown of the conductive network in Si anodes and the formation of an unstable solid-electrolyte interface,resulting in capacity fading.Here,we demonstrate nanoparticles with a Si@Mn_(22.6)Si_(5.4)C_4@C double-shell structure and the formation of self-organized Si-Mn-C nanocomposite anodes during the lithiation/delithiation process.The anode consists of amorphous Si particles less than 10 nm in diameter and separated by an interconnected conductive/buffer network,which exhibits excellent charge transfer kinetics and charge/discharge performances.A stable specific capacity of 1100 mAh·g^(-1) at 100 mA·g^(-1) and a coulombic efficiency of 99.2%after 30 cycles are achieved.Additionally,a rate capacity of 343 mAh·g^(-1) and a coulombic efficiency of 99.4%at 12000 mA·g^(-1) are also attainable.Owing to its simplicity and applicability,this strategy for improving electrode performance paves a way for the development of high-performance Si-based anodic materials for lithium ion batteries. | Xiuyun Zhao Dingguo Xia Lin Gu Juncheng Yue Biao Li Hang Wei Huijun Yan Ruqiang Zou Yingxia Wang Xiayan Wang Ze Zhang Jixue Li | 2014 | Journal of Energy Chemistry2014，23，3： | 1 |
| 12 | Genetic mapping and localization of a major QTL for seedling resistance to downy mildew in Chinese cabbage (Brassica rapa ssp. pekinensis)显示文摘 | Shuancang Yu Fenglan Zhang Renbo Yu Yanmin Zou Jiani Qi Xiuyun Zhao Yangjun Yu Deshuang Zhang Li Li | 2009 | Molecular Breeding2009，，4： | 1 |
| 13 | On the Normal Subgroup with Exactly Two G-Conjugacy Class Sizes显示文摘Let G be a finite group with a non-central Sylow r-subgroup R, Z(G) the center of G, and N a normal subgroup of G. The purpose of this paper is to determine the structure of N under the hypotheses that N contains R and the G-conjugacy class size of every element of N is either 1 or m. Particularly, it is shown that N is Abelian if N ∩ Z(G) = 1 and the G-conjugacy class size of every element of N is either 1 or m. | Xianhe ZHAO Xiuyun GUO | 2009 | Chinese Annals of Mathematics,Series B2009，30，4： | 1 |
| 14 | Parental HIV/AIDS and psychosocial adjust ment among rural Chinese children 显示文摘 | Fang Xiaoyi Li Xiaoming Stanton Bonita Hong Yan Zhang Liying Zhao Guoxiang Zhao Junfeng Lin Xiuyun Lin Danhua | 2009 | Journal of Pediatric Psychology2009，，3410： | 1 |
| 15 | Positive Future Orientation as a Mediator between Traumatic Events and Mental Health Among Children Affected by HIV/AIDS in Rural China显示文摘 | Zhang Jintao Zhao Guoxiang Li Xiaoming Hong Yan Fang Xiaoyi Barnett D Lin Xiuyun Zhao Junfeng Zhang Liying | 2009 | Aids care-psychological and socio-medical aspects of AID/HIV2009，21，12： | 1 |
| 16 | On p-regular G-conjugacy classes and the p-structure of normal subgroups 显示文摘 | GUO Xiuyun ZHAO Xianhe SHUM K P | 2009 | Communications in Algebra2009，37，： | 1 |
| 17 | Bacillus thuringiensis peptidoglycan hydrolase SleB171 involved in daughter cell separation during cell division显示文摘整个染色体的分析揭示了在杆菌 thuringiensis BMB171 与未知功能的二另外的基因(ypeB 和 yhcN ) 组成操纵子的通常认为的房间墙 hydrolase 基因(sleB171 ) 。通常认为的 SleB171 蛋白质由 259 氨基酸组成并且有 28.3 kDa 的分子的重量。在 BMB171 染色体的 sleB171 的基因混乱在植物的生长阶段和延期孢子形成和孢子版本期间引起长细胞链的形成,尽管它没在细胞生长和孢子的最终的版本上有重要效果。无法分开的植物的房间将近通过 sleB171 表示的互补被恢复。实时量的聚合酶链反应分析表明 sleB171 在植物的生长阶段主要是活跃的,与在早静止的生长阶段的一项最大的活动。西方的污点分析也证实 sleB171 优先地在植物的生长阶段期间被表示。这些结果证明 SleB171 在房间分割期间在女儿房间分离起一个必要作用。 | Hua Li Penggao Hu Xiuyun Zhao Ziniu Yu Lin Li | 2016 | Acta Biochimica et Biophysica Sinica2016，48，4： | 0 |
| 18 | Kinship,Equality,and Hierarchy:Sex Distinction and Values in Comparative Perspective①显示文摘Equality is a recurrent theme in discussions of Euro-American societies. It is both an aspiration and an impossibility which stands in contradistinction to Euro-American notions of power (Dumont 1 9 8 6; Robbins 1994). As an aspiration, it goes in tandem with the idea of liberty,both linked to the value of individualism, which prevails in these societies. | Cecile Barraud Zhao Xiuyun | 2016 | 民族学刊2016，7，3： | 0 |
| 19 | Anti-oxidative effect of ribonuclease inhibitor by site-directed mutagenesis and expression in Pichia pastoris显示文摘Human placental ribonuclease inhibitor(hRI)is an acidic protein of Mr∼50kDa with unusually high contents of leucine and cysteine residues.It is a cytosolic protein that protects cells from the adventitious invasion of pancreatic-type ribonuclease.hRI has 32 cysteine residues,and the oxidative formation of disulfide bonds from those cysteine residues is a rapid cooperative process that inactivates hRI.The most proximal cysteine residues in native hRI are two pairs that are adjacent in sequence.In the present aork,two molecules of alanine substituting for Cys328 and Cys329 were performed by site-directed mutagenesis.The site-mutated RI cDNA was constructed into plasmid pPIC9K and then transformed Pichia pastoris GS115 by electroporation.After colony screening,the bacterium was cultured and the product was purified with affinity chromatography.The affinity of the recombinant human RI with double site mutation was examined for RNase A and its anti-oxidative effect.Results indicated that there were not many changes in the affinity for RNase A detected when compared with the wild type of RI.But the capacity of anti-oxidative effect increased by 7~9 times.The enhancement in anti-oxidative effect might be attributed to preventing the formation of disulfide bond between Cys328 and Cys329 and the three dimensional structure of RI was thereby maintained. | Wu Yu Cui Xiuyun Wang Jihong Zhao Peng Xu Yuefei Zhao Baochang | 2006 | Frontiers in Biology2006，1，2： | 0 |
| 20 | Abrin P2 suppresses proliferation and induces apoptosis of colon cancer cells via mitochondrial membrane depolarization and caspase activation显示文摘在人的结肠癌 HCT-8 房间上探索 abrin P2 的细胞毒素的机制, abrin P2 从 Abrus precatorius L 的种子被孤立。abrin P2 向 12 根不同人的癌症房间线展出了 cytotoxicity,这被发现。我们的结果证明 abrin P2 压制了人的结肠癌房间(HCT-8 房间) 的增长并且在 S 和 G2/M 阶段导致了房间周期拘捕。abrin P2 由禁止了房间增长的机制经由 cyclin B1 的下面规定,增殖房间原子抗原和 Ki67,以及 P21 的起来规定。另外, abrin P2 处于 HCT-8 房间 apoptosis 的率导致了剂量依赖者和时间依赖者增加。有 Z-VAD-FMK,一个用途广泛的 caspase 禁止者,和 abrin P2 的治疗证明 abrin P2 经由 caspases 的激活导致了 HCT-8 房间 apoptosis。一起,我们的结果揭示了那 abrin 在 HCT-8 房间的导致 P2 的 apoptosis 与 caspases-3/-8/-9,在 Bcl-2/Bax 比率的减小, mitochondrial 膜潜力的损失,和细胞色素 c 的增加的激活被联系版本。我们进一步证明 abrin P2 管理有效地在裸体老鼠压制了结肠癌异种皮移植的生长。这是 abrin P2 有效地由压制增长并且导致 apoptosis 在 vivo 并且在 vitro 禁止结肠癌细胞生长的第一份报告。 | Ying Yu Runmei Yang Xiuyun Zhao Dandan Qin Zhaoyang Liu Fang Liu Xin Song Liqin Li Renqing Feng Nannan Gao | 2016 | Acta Biochimica et Biophysica Sinica2016，48，5： | 0 |
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