|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Discovery of mitochondrial chimeric-gene associated with cytoplasmic male sterility of HL-rice显示文摘The mitochondrial genome libraries of HL-type sterile line(A) and maintainer line(B) have been constructed. Mitochondrial gene, atp6, was used to screen libraries, due to the different Southern and Northern blot results between sterile and maintainer line. Sequencing analysis of positive clones proved that there were two copies of atp6 gene in sterile line and only one in maintainer line. One copy of atpt6 in sterile line was same to that in maintainer line; the other showed different flanking sequence from the 49th nucleotide downstream of the termination codon of atp6 gene. A new chimeric gene, orfH79, was found in the region. OrfH79 had homology to mitochondrial gene cox Ⅱ and orf107, and was special to HL-sterile cytoplasm. | Yl Ping WANG Li SUN Qingping ZHU Yingguo | 2002 | Chinese Science Bulletin2002，47，9： | 36 |
| 2 | Development of japonica Photo-Sensitive Genic Male Sterile Rice Lines by Editing Carbon Starved Anther Using CRISPR/Cas9显示文摘Rice is one of the most important crops as it supports over25%of total caloric intake for humans(Kusano et al.,2015).The world population reached 7.3 billion in 2015 and is projected to reach 8.5 billion in 2030(Word Population Prospects:2015 Revision).To meet the food demands for the everincreasing population with limited arable land,scarce | Quanlin Li Dabing Zhang Mingjiao Chen Wanqi Liang Jiaojun Wei Yiping Qi Zheng Yuan | 2016 | Journal of Genetics and Genomics2016，43，6： | 26 |
| 3 | ZmMs30 Encoding a Novel GDSL Lipase Is Essential for Male Fertility and Valuable for Hybrid Breeding in Maize显示文摘Genic male sterility (GMS) is very useful for hybrid vigor utilization and hybrid seed production. Although a large number of GMS genes have been identified in plants, little is known about the roles of GDSL lipase members in anther and pollen development. Here, we report a maize GMS gene, ZmMs30, which encodes a novel type of GDSL lipase with diverged catalytic residues. Enzyme kinetics and activity assays show that ZmMs30 has lipase activity and prefers to substrates with a short carbon chain. ZmMs30 is specifically expressed in maize anthers during stages 7-9. Loss of ZmMs30 function resulted in defective anther cuticle, irregular foot layer of pollen exine, and complete male sterility. Cytological and lipidomics analyses demonstrate that ZmMs30 is crucial for the aliphatic metabolic pathway required for pollen exine formation and anther cuticle development. Furthermore, we found that male sterility caused by loss of ZmMs30 function was stable in various inbred lines with different genetic background, and that it didn't show any negative effect on maize heterosis and production, suggesting that ZmMs30 is valuable for crossbreeding and hybrid seed production. We then developed a new multi-control sterility system using ZmMs30 and its mutant line, and demonstrated it is feasible for generating desirable GMS lines and valu. able for hybrid maize seed production. Taken together, our study sheds new light on the mechanisms of anther and pollen development, and provides a valuable male-sterility system for hybrid breeding maize. | Xueli An Zhenying Dong Youhui Tian Ke Xie Suowei Wu Taotao Zhu Danfeng Zhang Van Zhou Canfang Niu Biao Ma Quancan Hou Jianxi Bao Simiao Zhang Ziwen Li Yanbo Wang Tingwei Yan Xiaojing Sun Yuwen Zhang Jinping Li Xiangyuan Wan | 2019 | Molecular Plant2019，12，3： | 15 |
| 4 | The molecular and evolutionary basis of reproductive isolation in plants显示文摘Reproductive isolation is defined as processes that prevent individuals of different populations from mating, survival or producing fertile offspring. Reproductive isolation is critical for driving speciation and maintaining species identity, which has been a fundamental concern in evolutionary biology. In plants,reproductive isolation can be divided into prezygotic and postzygotic reproductive barriers, according to its occurrence at different developmental stages. Postzygotic reproductive isolation caused by reduced fitness in hybrids is frequently observed in plants, which hinders gene flow between divergent populations and has substantial effects on genetic differentiation and speciation, and thus is a major obstacle for utilization of heterosis in hybrid crops. During the past decade, China has made tremendous progress in molecular and evolutionary basis of prezygotic and postzygotic reproductive barriers in plants. Present understandings in reproductive isolation especially with new data in the last several years well support three evolutionary genetic models, which represent a general mechanism underlying genomic differentiation and speciation. The updated understanding will offer new approaches for the development of wide-compatibility or neutral varieties, which facilitate breeding of hybrid rice as well as other hybrid crops. | Yidan Ouyang Qifa Zhang | 2018 | Journal of Genetics and Genomics2018，45，11： | 9 |
| 5 | Development of the “Third-Generation” Hybrid Rice in China显示文摘Rice is a major cereal crop for China. The development of the ‘‘three-line' hybrid rice system based on cytoplasmic male sterility in the 1970 s(first-generation) and the ‘‘two-line' hybrid rice system based on photoperiod-and thermo-sensitive genic male-sterile lines(second-generation)in the 1980 s has contributed significantly to rice yield increase and food security in China. Here we describe the development and implementation of the ‘‘third-generation' hybrid rice breeding system that is based on a transgenic approach to propagate and utilize stable recessive nuclear male sterile lines, and as such, the male sterile line and hybrid rice produced using such a system is nontransgenic. Such a system should overcome the intrinsic problems of the ‘‘first-generation' and‘‘second-generation' hybrid rice systems and hold great promise to further boost production of hybrid rice and other crops. | Haiyang Wang Xing Wang Deng | 2018 | Genomics, Proteomics & Bioinformatics2018，16，6： | 8 |
| 6 | Molecular mechanisms of hybrid sterility in rice显示文摘Hybrid sterility presents a major bottleneck in hybrid crop breeding and causes postzygotic reproductive isolation in speciation.Here, we summarize the current understanding of the genetics of rice hybrid sterility and highlight new advances in deciphering the molecular basis of the major genetic loci for hybrid sterility in rice. We also discuss practical strategies for overcoming reproductive barriers to utilize hybrid vigor in inter-specific and inter-subspecific hybrid rice breeding. | Yongyao Xie Rongxin Shen Letian Chen Yao-Guang Liu | 2019 | Science China(Life Sciences)2019，62，6： | 8 |
| 7 | Mapping of the nuclear fertility restorer gene for HL cytoplasmic male sterility in rice using microsatellite markers显示文摘Bulked segregant analysis (BSA) of a BC1 population derived from Congguang 41A//Miyang 23/Congguang 41B was used to map the nuclear fertility restorer gene for Honglian (HL) cytoplasmic male sterility. One hundred and fifty-nine microsatellite primer pairs were screened for polymorphisms between the parents and between two bulks representing fertile and sterile plants. One microsatellite marker RM258 produced polymorphic products. The nuclear fertility restorer gene for HL cytoplasmic male sterility was mapped on chromosome 10, 7.8cM from RM258. The restorer gene may be clustered on chromosome. | HUANG Qingyang HE Yuqing JING Runchun ZHU Renshan ZHU Yingguo | 2000 | Chinese Science Bulletin2000，45，5： | 8 |
| 8 | Sequence analysis of the gene correlated with cytoplasmic male sterility (CMS) in rape-seed (Brassica napus) Polima and Shaan 2A显示文摘orf224 is a CMS-related mitochondrial gene discovered in Polima cytoplasm. Shaan 2A CMS line is the parent of the first rapeseed hybrid cultivar Qinyou No. 2 that has been grown in many regions of China. In this work, genomic DNA of Polima CMS line and Shaan 2A CMS line were used as templates, two primers of specific oligonucleo-tides at 5’ and 3’ ends were used, PCR was performed, the amplification fragments were cloned into pGEM-T Easy vectors and DNA sequences were determined. The CMS-associated gene, orf224-1 present in Shaan 2A CMS line, has a sequence highly homologous to the orf224 of the Polima CMS line, except for one nucleotidc at position +398. There were only one base (AAC→AGC) and one amino acid (Asn→Ser) differences between the two. The homologies of the two sequences in nucleotide and amino acid were 99.9% and 99.6%, respectively. It is concluded that orf224 in Polima CMS line and orf224-1 of Shaan 2A CMS line are the allele at the same locus in mitochondria. | WANG Yongfei Ma Sanmei WANG Ming ZHENG Xueqin GU Mao HU Shengwu | 2002 | Chinese Science Bulletin2002，47，2： | 7 |
| 9 | Identifi cation of a novel male sterile wheat mutant dms conferring dwarf status and multi-pistils显示文摘Plant height and fertility are two important traits of wheat(Triticum aestivum L.), whose mutants are ideal materials for studies on molecular mechanisms of stem and floral organ development. In this study, we identified a dwarf, multi-pistil and male sterile(dms hereafter) wheat mutant from Zhoumai 18. Simple sequence repeat(SSR) marker assay with 181 primer pairs showed that only one locus of GWM148-2B was divergent between Zhoumai 18 and dms. There were three typical phenotypes in the progeny of dms, tall(T; ca. 0.8 m), semi-dwarf(M; ca. 0.6 m) and dwarf(D; under 0.3 m) plants. Morphological investigation indicated that the internode length of M was shortened by about 20–50 mm each; the internode number of D was 2 less than that of T and Zhoumai 18, and its internode length was shorter also. The pollen vigor and hybridization test demonstrated that dms mutant was male sterility. Segregated phenotypes in progeny of M suggested that the multi-pistils and sterility were controlled by one recessive gene locus which was designated as dms temporarily, and the plant height was controlled by a semi-dominant gene locus Dms. Therefore, progeny individuals of the dms had three genotypes, Dms Dms for tall plants, Dmsdms for semi-dwarf plants and dmsdms for dwarf plants. The mutant progenies were individually selected and propagated for more than 6 generations, thus a set of near isogenic lines of T, M and D for dms were developed. This study provides a set germplasms for studies on molecular mechanisms of wheat stem and spike development. | DUAN Zong-biao SHEN Chun-cai LI Qiao-yun Lü Gui-zhen NI Yong-jing YU Dong-yan NIU Ji-shan | 2015 | Journal of Integrative Agriculture2015，14，9： | 6 |
| 10 | 玉米S型细胞质雄性不育恢复基因Rf3的精细定位及其候选基因预测显示文摘细胞质雄性不育(Cytoplasmic male sterility,CMS)广泛存在于高等植物,CMS-S是玉米CMS的一种,其育性可以被恢复基因Rf3完全恢复。目前Rf3基因尚未成功克隆。本试验根据玉米CMS-S配子体不育特点,提出一种全新的同质群体构建方法,通过该方法获得群体的所有个体均携带Rf3恢复基因,省去田间表型鉴定工作,并可在同一世代获得足够的重组个体。该方法可直接用种子提取基因组DNA,大大提高Rf3定位的效率。利用该同质定位群体,将恢复基因Rf3精细定位到分子标记A165与CG2之间,参照玉米自交系B73的基因组序列,两个标记之间的物理距离约为1.4 Mb。 | 李鹏 肖森林 王淑霞 刘娟 赵贤容 陈化榜 | 2014 | 山东农业科学2014，46，8： | 5 |
| 11 | A PRELIMINARY REPORT ON MALE STERILITY IN RICE, Oryza sativa L.显示文摘Through panicle to panicle inspection at heading stage, six male-sterile rice plants were found in the paddy fields where several varieties were grown from 1964 to 1965 in Hunan Province. The frequency of occurrence of such male-sterile rice plants under natural conditions was estimated to be 0.13 per cent. According to the characteristics of their anthers and pollen grains, these male-sterile plants can be distinguished into three different types: (1) Pollen-free type. Two plants of this type were found to be completely malesterile. Their anthers are smaller and thinner than those of the normal plant as shown in Fig. 1. They are colourless, remain unbroken after flowering, and contain no pollen grains. | 袁隆平 | 1966 | Chinese Science Bulletin1966，24，7： | 5 |
| 12 | Gene characterization and molecular pathway analysis of reverse thermosensitive genic male sterility in eggplant(Solanum melongena L.)显示文摘The naturally occurring mutant eggplant line 05ms was identified with reverse thermosensitive genic male sterility(rTGMS),but its temperature-responsive fertility mechanisms remain largely unknown.Here,we studied the flower morphology,anther cellular structure,and genome-wide gene expression of this rTGMS line.Candidate genes for thermosensitive male sterility during the microspore development of 05ms and the temperature-insensitive line S63 under low-temperature(LT)and high-temperature(HT)conditions were identified.Under LT,tapetum cells were vacuolated and had delayed disintegration in 05ms.RNA-seq analysis indicated that DEGs were enriched in the KEGG pathways‘plant hormone signal transduction’,‘starch and sucrose metabolism’,and‘phenylpropanoid biosynthesis’.We identified two genes,4CLL1(Sme2.5_00368.1_g00010.1)and CKI1(Sme2.5_10056.1_g00002.1),which could potentially regulate eggplant anther development and may be candidate genes for rTGMS.Finally,we propose a working model of anther abortion for rTGMS in eggplant.CKI1 responds to LT stress and causes expression changes in genes related to anther development,such as 4CLL1,and the cellular structure of the tapetum becomes abnormal,causing male sterility.The findings of this study explain the underlying molecular mechanisms of male sterility in eggplant rTGMS lines. | Bing Li Xueping Chen Yanrong Wu Aixia Gu Jingjing Zhang Shuangxia Luo Xiurui Gao Jianjun Zhao Xiuqing Pan Shuxing Shen | 2019 | Horticulture Research2019，6，1： | 5 |
| 13 | Identifying and mapping cDNA fragments related to rice photoperiod sensitive genic male sterility显示文摘The differentially expressed cDNA fragments have been obtained by differential screening with cDNA-RAPD technique in photoperiod sensitive genic male sterile (PGMS) rice. Some of them have been reassessed with Northern blot hybridization, from which a PGMS-related positive fragment, RPG43, has been identified. Further analysis on RPG43 with Southern blot and RAPD indicates that the fragment is a single-copy sequence and its mRNA has been processed after transcription. Sequence analysis reveals that RPG43 is 744 bp in length and contains a 60 bp region (from 126th to 185th bp) showing 72% homology to a human DNA sequence, pac pDJ-356d6, on chromosome 11. So it is a new sequence found in plant and its GenBank access number is AF126027. In addition, RPG43 has been mapped to a position 3.8 cM away from RFLP marker R1553 on chromosome 5 of rice. | JIANG Shuye CHEN Qifeng FANG Xuanjun | 2000 | Chinese Science Bulletin2000，45，6： | 4 |
| 14 | Inheritance and mapping of embryo sac abortion in hybrid between Indica and Japonica rice (Oryza sativa L.)显示文摘A doubled haploid (DH) population derived from anther culture of F\-1’s between an Indica var. Zhai\|Ye\|Qing 8 and a Japonica var. Jing\|Xi17 as well as two backcross populations derived from this DH population were used to investigate inheritance of the embryo sac abortion at early megagametogenesis occurring in Indica/Japonica rice crosses. Two major loci, dominant and complementary \%esa\|1\% and \%esa\|2\%, located on chromosomes 6 and 12 respectively, were detected. Genetic analysis indicated that embryo sac fertility is mainly regulated by the gametophytic genotype at these two loci. | 刘永胜 朱立煌 孙敬三 沈利爽 王玉忠 何平 陆朝福 陈英 | 1998 | Science China(Life Sciences)1998，41，3： | 3 |
| 15 | Characterization of H3 methylation in regulating oocyte development in cyprinid fish显示文摘Histone post-modifications are important epigenetic markers involved in multiple cellular processes via regulation of gene transcription or remodeling of chromatin structure. Oocyte development is a critical process under rigorous control to prevent the generation of aberrant gametes. However, the regulatory mechanism of oocyte early development is not well-understood due to the tiny size and poor distinguishability of the gonad in juvenile stages. Here, two cyprinid hybrid fishes, a sterile allotriploid fish and a gynogenetic hybrid fish with delayed oocyte development, provided research models to investigate the mechanisms involved. We used cytogenetic and molecular methods to confirm the pachytene arrest of oocytes in allotriploid fish and gynogenetic hybrid fish. On the basis of these developmental differences, we screened 21 different histone H3 modifications by ELISA and found that four modifications(H3 K4 me3, H3 K9 me3, H3 K79 me, and H3 K79 me3) differed significantly in the two cyprinid hybrid fishes. Changes in histone methylation at the three residues(H3 K4, K9, K79) were caused by specific methyltransferases and demethylases. Our results provide new insights into the epigenetic regulation of oocyte early development in fish, a process critical for understanding of reproductive biology and with practical applications in the aquacultural breeding industry. | Rong Zhou Rujie Shang Dingbin Gong Xiujuan Xu Shaojun Liu | 2019 | Science China(Life Sciences)2019，62，6： | 3 |
| 16 | Disruption of the bHLH transcription factor Abnormal Tapetum 1 causes male sterility in watermelon显示文摘Although male sterility has been identified as a useful trait for hybrid vigor utilization and hybrid seed production,its underlying molecular mechanisms in Cucurbitaceae species are still largely unclear.Here,a spontaneous male-sterile watermelon mutant,Se18,was reported to have abnormal tapetum development,which resulted in completely aborted pollen grains.Map-based cloning demonstrated that the causal gene Citrullus lanatus Abnormal Tapetum 1(ClATM1)encodes a basic helix-loop-helix(bHLH)transcription factor with a 10-bp deletion and produces a truncated protein without the bHLH interaction and functional(BIF)domain in Se18 plants.qRT–PCR and RNA in situ hybridization showed that ClATM1 is specifically expressed in the tapetum layer and in microsporocytes during stages 6–8a of anther development.The genetic function of ClATM1 in regulating anther development was verified by CRISPR/Cas9-mediated mutagenesis.Moreover,ClATM1 was significantly downregulated in the Se18 mutant,displaying a clear dose effect at the transcriptional level.Subsequent dual-luciferase reporter,β-glucuronidase(GUS)activity,and yeast one-hybrid assays indicated that ClATM1 could activate its own transcriptional expression through promoter binding.Collectively,ClATM1 is the first male sterility gene cloned from watermelon,and its self-regulatory activity provides new insights into the molecular mechanism underlying anther development in plants. | Ruimin Zhang Jingjing Chang Jiayue Li Guangpu Lan Changqing Xuan Hao Li Jianxiang Ma Yong Zhang Jianqiang Yang Shujuan Tian Li Yuan Xian Zhang Chunhua Wei | 2021 | Horticulture Research2021，8，1： | 3 |
| 17 | 烟草重要基因篇:9.烟草雄性不育和育性恢复基因显示文摘植物雄性不育(male sterility,MS)既是植物生殖生物学研究中的一个重要植物学性状,也是研究作物杂种优势利用的一个重要农艺性状,在作物育种和分子生物学研究中具有重要地位。植物雄性不育可分为细胞核雄性不育(genic male sterility,GMS)和细胞质雄性不育(cytoplasmic male sterility,CMS)。GMS由核不育基因控制,无正反交遗传效应;CMS由线粒体不育基因和核恢复基因(restorer of fertility,Rf)共同调控,线粒体基因导致不育,Rf能改变线粒体中不育基因的表达,使不育系恢复育性。 | 李凤霞 | 2015 | 中国烟草科学2015，36，3： | 3 |
| 18 | CHLOROPLAST AND CYTOPLASMIC MALE-STERILITY IN MAIZE显示文摘Chloroplast is one of the organelles which possesses the function of energy convertion specific for green cells and replication, transcription and translation system of its own DNA. It plays an important role in the life of green plants. Cytoplasmic male-sterility (CMS) is one of the forms of extranuclear inheritance.The genetic mechanism of the CMS has been explained by the hypotheses of 'Cytoplasmic genes' or 'Plastons' for a long time. So that the cause of CMS formation remains unknown until now. | 刘一农 李继耕 | 1983 | Chinese Science Bulletin1983，28，11： | 2 |
| 19 | Microsatellite-Aided Screening for Fertility Restoration Genes (Rf) Facilitates Hybrid Improvement显示文摘DNA markers enabled to determine the chromosomal locations of the two Rf genes(Rf3 and Rf4) in the wild-abortive cytoplasmic male sterility(WA-CMS) system. Four simple sequence repeats(SSRs) RM171, RM258, RM315 and RM443 were used to detect the allelic status with respect to the fertility restoration genes(Rf3 and Rf4) in 300 rice cultivars or breeding lines. The results revealed that out of 300 lines, 90 lines screened had Rf3, 65 lines had Rf4, and 45 lines had Rf3 and Rf4 alleles. Furthermore, 45 lines selected using SSR markers were mated with a CMS line(IR58025A) to analyze their restoring ability. Offspring of all the test lines except HHZ8-SAL9DT1-Y1, HHZ5-SAL9-Y3-1 and IDSA77 exhibited higher pollen and spikelet fertility(> 80%), thus confirming they bear the Rf alleles. The hybrid offspring of ARH12-6-1-1-B-3-1, IR32307-10-3-2-1 and Sahel 329 had the highest pollen fertility(97.39%, 98.30% and 97.10%, respectively) and spikelet fertility(95.10%, 97.07% and 96.10%, respectively). | Raafat El-Namaky Saber Sedeek Yonnelle Dea Moukoumbi Rodomiro Ortiz Baboucarr Manneh | 2016 | Rice science2016，23，3： | 2 |
| 20 | Effect of HSP70 antisense RNA on normal sorghum pollen formation显示文摘IT has been found that heat shock proteins (hsp) exist universally in animals, plants, microbesand human beings. Some of hsps possess functions of molecular chaperons, such as hsp60,hsp70 and hsp90, which participate in folding and assembling polypeptides to functional struc-tures and transferring them into suitable sites. They also undertake repairing damaged cellularDNA and nucleolus. Therefore, they are house-keeping proteins and play an importantrole in maintaining normal cellular life and functions and in cellular response to environmentalstresses. There exists high homology in DNA sequence among the hsp of the same molecularweight across species. We have first reported the close relationship between hsps | Chen, JN Fu, HY Lu, ZX Qin, HY Zhang, L Guo, ZB | 1997 | Chinese Science Bulletin1997，42，23： | 2 |
您所在的机构暂未开通此部分的功能，请联系管理员