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| 1 | Narrow line between benefit and harm: Additivity of hyperthermia to cisplatin cytotoxicity in different gastrointestinal cancer cells显示文摘AIM To investigate the response to hyperthermia and chemotherapy, analyzing apoptosis, cytotoxicity, and cisplatin concentration in different digestive system cancer cells.METHODS AGS(gastric cancer cell line), Caco-2(colon cancer cell line) and T3M4(pancreatic cancer cell line) were treated by cisplatin and different temperature setting(37 ℃ to 45 ℃) either in isolation, or in combination. Treatment lasted for one hour. 48 h after the treatment viability was evaluated by MTT, cell apoptosis by Annexin V-PE and 7 ADD flow cytometry. Intracellular cisplatin concentration was measured immediately after the treatment, using mass spectrometry. Isobologram analysis was performed to evaluate the mathematical combined effect of temperature and cisplatin.RESULTS AGS cells were the most sensitive to isolated application of hyperthermia. Hyperthermia, in addition to cisplatin treatment, did not provoke a synergistic effect at intervals from 37 ℃ to 41 ℃ in neither cancer cell line. However, a temperature of 43 ℃ enhanced cisplatin cytotoxicity for Caco-2 cells. Moreover, isobologram analysis revealed mathematical antagonistic effects of cisplatin and temperature combined treatment in AGS cells; variations between synergistic, additive, and antagonistic effects in Caco-2 cells; and additive and antagonistic effects in T3 M4 cells. Combined treatment enhanced initiation of cell apoptosis in AGS, Caco-2, and T3 M4 cells by 61%, 20%, and 19% respectively. The increase of intracellular cisplatin concentration was observed at 43 ℃ by 30%, 20%, and 18% in AGS, Caco-2, and T3 M4 cells, respectively.CONCLUSION In addition to cisplatin, hyperthermia up to 43 ℃ does not affect the viability of cancer cells in a synergistic manner. | Vaidotas Cesna Arturas Sukovas Aldona Jasukaitiene Rima Naginiene Giedrius Barauskas Zilvinas Dambrauskas Saulius Paskauskas Antanas Gulbinas | 2018 | World Journal of Gastroenterology2018，24，10： | 6 |
| 2 | HuR mediated post-transcriptional regulation as a new potential adjuvant therapeutic target in chemotherapy for pancreatic cancer显示文摘AIM: To investigate the expression of Hu R in pancreatic ductal adenocarcinoma(PDA) and to assess the effects of Hu R silencing on the expression of cyclooxygenase-2(COX-2) and heme oxygenase-1(HO-1) and the in vitro response to gemcitabine(GEM) treatment in pancreatic cell lines.METHODS: We compared the expression of Hu R,COX-2,and HO-1 in PDA and normal pancreatic tissueusing quantitative reverse transcription polymerase chain reaction(q RT-PCR) and western blot. In addition,the Hu R,COX-2 and HO-1 were analyzed in four types of cancer cell lines(Mia Paca2,Su.86.86,Capan-1,and Capan-2) with and without GEM treatment. Immunocytofluorescence analysis was used to investigate Hu R localization in cells. Cell viability and response to GEM after Hu R silencing were determined with the 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide test and the crystal violet clonogenic assay,respectively. To measure apoptosis,activation of caspases 3/7 was evaluated using immunofluorescence. RESULTS: In PDA tissue obtained from patients not treated with GEM,Hu R m RNA expression was 3.2 times lower(P < 0.05) and COX-2 and HO-1 m RNAexpression was 2.3-fold and 7.2-fold higher(P < 0.05),respectively,than normal pancreatic tissue(from organ donor). q RT-PCR analysis showed that Hu R,COX-2,and HO-1 m RNA were overexpressed in all cancer cell lines treated with the half maximal inhibitory concentration(IC50) dose of GEM compared with control cells(P < 0.05). Western blot analysis revealed that COX-2 and HO-1 levels were significantly decreased in cancer cells after Hu R silencing. Furthermore,HuR silencing in creased the response to GEM treatmentand decreased cell viability by 11.6%-53.7% compared to control cell lines. Caspases 3 and 7 were activated after Hu R silencing and GEM treatment in all pancreatic cancer cell lines. In comparison,treatment with GEM alone did not activate caspases 3 and 7 in the same cell lines. CONCLUSION: Hu R mediated post-transcriptional upregulation of COX-2 and HO-1 expression after GEM treatment in pancreatic cancer cells. Hu R silencing significantly increased the effectiveness of GEM treatment in vitro. | Aldona Jakstaite Aurelija Maziukiene Giedre Silkuniene Kristina Kmieliute Antanas Gulbinas Zilvinas Dambrauskas | 2015 | World Journal of Gastroenterology2015，21，46： | 4 |
| 3 | Glucagon-like peptide-1 receptor imaging with [Lys 40 (Ahx-HYNIC- 99m Tc/EDDA)NH 2 ]-exendin-4 for the detection of insulinoma显示文摘 | Anna Sowa-Staszczak Dorota Pach Renata Miko?ajczak Helmut M?cke Agata Jabrocka-Hybel Agnieszka Stefańska Monika Tomaszuk Barbara Janota Aleksandra Gilis-Januszewska Maciej Ma?ecki Grzegorz Kamiński Aldona Kowalska Jan Kulig Andrzej Matyja Czes?aw Osuch Al | 2013 | European Journal of Nuclear Medicine and Molecular Imaging2013，，4： | 2 |
| 4 | Stability of poly(vinylidene fluoride hexafluoropropylene) based composite gel electrolytes with funetionalized silicas显示文摘 | Mariusz Walkowiak Aldona Zalewska Teofil Jesionowski | 2007 | Journal of Power Sources2007，173，2： | 1 |
| 5 | Tail behavior of random sums under consistent variation with applications to the compound renewal risk model显示文摘 | Aldona Ale?kevi?ien? Remigijus Leipus Jonas ?iaulys | 2008 | Extremes2008，，3： | 1 |
| 6 | Calcium hydroxyapatite Ca10(PO4)6(OH)2 ceramics prepared by aqueous sol-gel processing显示文摘 | Irma Bogdanoviciene Aldona Begauskiene Jochen Glaser | 2006 | Materials Research Bulletin2006，41，9： | 1 |
| 7 | Frequent hypermethylation of DAPK , RARbeta , MGMT , RASSF1A and FHIT in laryngeal squamous cell carcinomas and adjacent normal mucosa显示文摘 | Jaros?aw Paluszczak Paulina Misiak Ma?gorzata Wierzbicka Aldona Wo?niak Wanda Baer-Dubowska | 2010 | Oral Oncology2010，，2： | 1 |
| 8 | Usefulness of C-reactive protein as a marker of early post-infarct left ventricular systolic dysfunction显示文摘 | Iwona Swiatkiewicz Marek Kozinski Przemyslaw Magielski Joanna Gierach Tomasz Fabiszak Aldona Kubica Adam Sukiennik Eliano Navarese Grazyna Odrowaz-Sypniewska Jacek Kubica | 2012 | Inflammation Research2012，，7： | 1 |
| 9 | Ntric oxide induces the synthesis of vascular endothelial growth facor by rat vascular smooth muscle cells显示文摘 | Jozef D Alicja J Aldona DK | 2000 | Artefioscler Thromb Vase Biol2000，20，3： | 1 |
| 10 | New oral anticoagulants-sunset for warfarin in therapy of atrial fib- rillation显示文摘 | Marek Kozifiski Karolina Oboftska Aldona | 2012 | Kardiologia Polska2012，70，10： | 1 |
| 11 | Differential expression of mucin 1 and mucin 2 in colorectal cancer显示文摘AIM To determine tissue expression(mRNA, protein) of two types of mucins [mucin 1(MUC1) and mucin 2(MUC2)] in patients with colorectal cancer(CRC).METHODS Expression of membrane-bound mucin(MUC1) and secretory mucin(MUC2) in CRC(mRNA, protein) were analyzed in tissue material including fragments of tumorsobtained from CRC patients(n = 34), and fragments of normal colorectal tissue from the same patients(control). The analysis was conducted using real-time quantitative polymerase chain reaction(RT-qPCR)(transcripts), immunohistochemistry(IHC)(apomucins), and the modern approach for morphometric analysis of IHC reaction(HSV filter software). Results on tissue expression of both mucins(mRNA, protein) were compared to histological alterations in colorectal cancer samples and correlated with selected clinical data in the patients. The statistical analysis was conducted using Statistica PL v. 12.0 software.RESULTS Significantly higher expression of the MUC1 mRNA in the CRC, compared with the control and the borderline correlation of mRNA expression with MUC1 protein levels in colorectal samples was observed. The expression of apomucins concerned cell membranes(MUC1) and cytoplasm(MUC2) and occurred both in control tissues and in most cancerous samples. There were no significant relationships between MUC1(mRNA, protein) and the clinicopathological data of patients. MUC2 protein expression was significantly lower as compared to the control, while MUC2 mRNA expression was comparable in both groups. The MUC1/MUC2 ratio was significantly higher in CRC tissues than in the control. The higher expression of MUC2 was a feature of mucinous CRC subtypes, and characterized higher histological stage of tumors. Negative correlations have been obtained between MUC2 and the Ki-67 antigen, as well as between MUC2 and p53 protein expressions in CRC.CONCLUSION A combination of tissue overexpression of MUC1, reduced MUC2 expression, and high ratio of MUC1/MUC2 is a factor of poor prognosis in CRC patients. MUC2 tissue expression allows to differentiate mucinous and nonmucinous CRC subtypes. | Aldona Kasprzak Elzbieta Siodla Malgorzata Andrzejewska Jacek Szmeja Agnieszka Seraszek-Jaros Szczepan Cofta Witold Szaflarski | 2018 | World Journal of Gastroenterology2018，24，36： | 1 |
| 12 | Calcium Hydroxy-apatite Ceramics Prepared Through Aqueous Sol-gel Processing显示文摘 | Irma Bogdanoviciene Aldona Began-skiene Kaia Tonsuaadu | 2006 | Materials Research Bulletin2006，41，9： | 1 |
| 13 | 478 Probe-Based Confocal Laser Endomicroscopy in Colonoscopic Surveillance of Patients With PSC-IBD显示文摘 | Aldona Dlugosz Ammar M. Barakat Ake Ost Annika Bergquist | 2013 | Gastroenterology2013，，5： | 1 |
| 14 | Differential expression of IGF-1 mRNA isoforms in colorectal carcinomaand normal colon tissue显示文摘 | Aldona Kasprzak Witold Szaflarski Jacek Szmeja Ma?gorzata Andrzejewska Wies?awa Przybyszewska El?bieta Kaczmarek Maria Koczorowska Tomasz Ko?ciński Maciej Zabel Micha? Drews | 2013 | International Journal of Oncology2013，，1： | 1 |
| 15 | The insulin-like growth factor (IGF) signaling axis and hepatitis Cvirus-associated carcinogenesis (Review)显示文摘 | Aldona Kasprzak Agnieszka Adamek | 2012 | International Journal of Oncology2012，，6： | 1 |
| 16 | Cytokines and anticytokines in psoriasis显示文摘 | Aldona T. Pietrzak Anna Zalewska Gra?yna Chodorowska Dorota Krasowska Anna Michalak-Stoma Piotr Nockowski Pawe? Osemlak Tomasz Paszkowski Jacek M. Roliński | 2008 | Clinica Chimica Acta2008，，： | 1 |
| 17 | Ghrelin and obestatin in thyroid gland - immunohistochemical expression in nodular goiter, papillary and medullary cancer显示文摘 | Gurgul Edyta Kasprzak Aldona Blaszczyk Agata Biczysko Maciej Surdyk-Zasada Joanna Seraszek-Jaros Agnieszka Ruchala Marek | 2015 | Folia Histochemica et Cytobiologica2015，，1： | 1 |
| 18 | Tail behavior of random sums under consistent variation with applications to the compound renewal risk model显示文摘 | Aldona Ale?kevi?ien? Remigijus Leipus Jonas ?iaulys | 2008 | Extremes2008，，3： | 1 |
| 19 | Stability of poly (vinylidene fluoride hexafluoroprop- ylene)-based composite gel electrolytes with functionalized silicas 显示文摘 | Mariusz Walkowiak Aldona Zalewska Teofil Jesionowski | 2007 | Journal of Power Sources2007，173，2： | 1 |
| 20 | Trends and dynamics of changes in calcification score over the 1-year observation period in patients on peritoneal dialysis显示文摘 | Tomasz P. Stompór Mieczys?aw Pasowicz W?adys?aw Su?owicz Aldona Dembińska-Kie? Katarzyna Janda Katarzyna Wójcik Wies?awa Tracz Anna Zdzienicka Ma?gorzata Konieczyńska Piotr Klimeczek Eve Janusz-Grzybowska | 2004 | American Journal of Kidney Diseases2004，，3： | 1 |
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