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| 1 | Antifibrogenic effects of vitamin D derivatives on mouse pancreatic stellate cells显示文摘AIM To study the molecular effects of three different D-vitamins, vitamin D2, vitamin D3 and calcipotriol, in pancreatic stellate cells(PSCs).METHODS Quiescent PSCs were isolated from mouse pancreas and activated in vitro by seeding on plastic surfaces. The cells were exposed to D-vitamins as primary cultures(early-activated PSCs) and upon re-culturing(fullyactivated cells). Exhibition of vitamin A-containing lipid droplets was visualized by oil-red staining. Expression of α-smooth muscle actin(α-SMA), a marker of PSC activation, was monitored by immunofluorescence and immunoblot analysis. The rate of DNA synthesis was quantified by 5-bromo-2'-deoxyuridine(Brd U) incorporation assays. Real-time PCR was employed to monitor gene expression, and protein levels of interleukin-6(IL-6) were measured by ELISA. Uptake of proline was determined using 18 F-proline.RESULTS Sustained culture of originally quiescent PSCs inducedcell proliferation, loss of lipid droplets and exhibition of stress fibers, indicating cell activation. When added to PSCs in primary culture, all three D-vitamins diminished expression of α-SMA(to 32%-39% of the level of control cells; P < 0.05) and increased the storage of lipids(scores from 1.97-2.15 on a scale from 0-3; controls: 1.49; P < 0.05). No such effects were observed when D-vitamins were added to fully-activated cells, while incorporation of Brd U remained unaffected under both experimental conditions. Treatment of re-cultured PSCs with D-vitamins was associated with lower expression of IL-6(-42% to-49%; P < 0.05; also confirmed at the protein level) and increased expression of the vitamin D receptor gene(209%-321% vs controls; P < 0.05). There was no effect of D-vitamins on the expression of transforming growth factor-β1 and collagen type 1(chain α1). The lowest uptake of proline, a main component of collagen, was observed in calcipotriol-treated PSCs.CONCLUSION The three D-vitamins inhibit, with similar efficiencies, activation of PSCs in vitro, but cannot reverse the phenotype once the cells are fully activated. | Peter Wallbaum Sarah Rohde Luise Ehlers Falko Lange Alexander Hohn Carina Bergner Sarah Marie Schwarzenbock Bernd Joachim Krause Robert Jaster | 2018 | World Journal of Gastroenterology2018，24，2： | 3 |
| 2 | Intestinal Tumorigenesis Initiated by Dedifferentiation and Acquisition of Stem-Cell-like Properties显示文摘 | Sarah Schwitalla Alexander A. Fingerle Patrizia Cammareri Tim Nebelsiek Serkan I. G?ktuna Paul K. Ziegler Ozge Canli Jarom Heijmans David J. Huels Guenievre Moreaux Rudolf A. Rupec Markus Gerhard Roland Schmid Nick Barker Hans Clevers Roland Lang Jens Neu | 2013 | Cell2013，，： | 3 |
| 3 | 台式液相色谱静电场轨道阱质谱联用系统显示文摘本文概述了基于具有卓越性能的Thermo Scientific Orbitrap静电场轨道阱质谱仪的新型Exactive台式质谱仪,对其主要的特性包括仪器设计、分析参数及应用进行了介绍。 | Andreas Wieghaus Alexander Makarov Ulf Froehlich Markus Kellmann Eduard Denisov Oliver Lange | 2011 | 分析测试学报2011，30，5： | 3 |
| 4 | Right Portal Vein Ligation Combined With In Situ Splitting Induces Rapid Left Lateral Liver Lobe Hypertrophy Enabling 2-Staged Extended Right Hepatic Resection in Small-for-Size Settings显示文摘 | Andreas A. Schnitzbauer Sven A. Lang Holger Goessmann Silvio Nadalin Janine Baumgart Stefan A. Farkas Stefan Fichtner-Feigl Thomas Lorf Armin Goralcyk Rüdiger H?rbelt Alexander Kroemer Martin Loss Petra Rümmele Marcus N. Scherer Winfried Padberg Alfred K? | 2012 | Annals of Surgery2012，，3： | 2 |
| 5 | From Today's Intranet of Things to A Future Internet of Things; A Wireless and Mobility-Related View显示文摘 | Miehele Zorzi Alexander Gtuhak Sebastian Lange Alessandro Bassi | 2010 | IEEE Wireless Communications2010，，05： | 1 |
| 6 | Excretion of carbimazole and propyhhiouracil in breast milk 显示文摘 | LOW L C LANG J ALEXANDER W D | 1979 | Lancet1979，2，8150： | 1 |
| 7 | Stability selection for genome-wide association 显示文摘 | Alexander D H Lange K | 2011 | Genet Epidemiol2011，35，7： | 1 |
| 8 | A system for retargeting of streaming video显示文摘 | Philipp Kr?henbühl Manuel Lang Alexander Hornung Markus Gross | 2009 | ACM Transactions on Graphics (TOG)2009，，5： | 1 |
| 9 | High prevalence of peripheral arterial disease and co-morbidity in 6880 primary care patients: cross-sectional study显示文摘 | Curt Diehm Alexander Schuster Jens R. Allenberg Harald Darius Roman Haberl Stefan Lange David Pittrow Berndt von Stritzky Gerhart Tepohl Hans-Joachim Trampisch | 2003 | Atherosclerosis2003，，1： | 1 |
| 10 | The WNT1^(G177C) mutation specifically affects skeletal integrity in a mouse model of osteogenesis imperfecta type XV显示文摘The recent identification of homozygous WNT1 mutations in individuals with osteogenesis imperfecta type XV(OI-XV)has suggested that WNT1 is a key ligand promoting the differentiation and function of bone-forming osteoblasts.Although such aninfluence was supported by subsequent studies,a mouse model of OI-XV remained to be established.Therefore,we introduced a previously identified disease-causing mutation(G177C)into the murine Wnt1 gene.Homozygous Wnt1^(G177C/G177C)mice were viable and did not display defects in brain development,but the majority of 24-week-old Wnt1^(G177C/G177C)mice had skeletal fractures.This increased bone fragility was not fully explained by reduced bone mass but also by impaired bone matrix quality.Importantly,the homozygous presence of the G177C mutation did not interfere with the osteoanabolic influence of either parathyroid hormone injection or activating mutation of LRP5,the latter mimicking the effect of sclerostin neutralization.Finally,transcriptomic analyses revealed that short-term administration of WNT1 to osteogenic cells induced not only the expression of canonical WNT signaling targets but also the expression of genes encoding extracellular matrix modifiers.Taken together,our data demonstrate that regulating bone matrix quality is a primary function of WNT1.They further suggest that individuals with WNT1 mutations should profit from existing osteoanabolic therapies. | Nele Vollersen Wenbo Zhao Tim Rolvien Fabiola Lange Felix Nikolai Schmidt Stephan Sonntag Doron Shmerling Simon von Kroge Kilian Elia Stockhausen Ahmed Sharaf Michaela Schweizer Meliha Karsak Bjorn Busse Ernesto Bockamp Oliver Semler Michael Amling Ralf Oheim Thorsten Schinke Timur Alexander Yorgan | 2021 | Bone Research2021，9，4： | 1 |
| 11 | Responsible research and innovation training programmes:implementation and evaluation of the HEIRRI project显示文摘Responsible research and innovation,or RRI,is a concept that aims to bring together society and science for a better future.There are six key elements of RRI:public engagement,gender equality,science education,open access,ethics and governance.Higher Education Institutions and Responsible Research and Innovation(HEIRRI)project aimed to bring the concept of RRI into the educational system.Using state-of-the-art review of good practices,HEIRRI team developed 10 training programmes on RRI for different higher education institution educational levels,including a summer school and a massive open online course(MOOC).We conducted pilot of the trainings and evaluated participants’experiences.Satisfaction with HEIRRI training programmes on responsible research and innovation was high,both for participants and for the trainers,and trainings raised awareness of RRI.Participants’feedback was used to identify areas that need improvement and provided for recommendations for final versions of the HEIRRI training programmes.In order to equip researchers with skills to recognize and apply RRI values,RRI should be included in their education.HEIRRI training is suitable for a range of different disciplines,including forensic science,and is free to use and adjust for specific contexts(available from:https://rri-tools.eu/heirri-training-programmes). | Ružica Tokalić Ivan Buljan Niels Mejlgaard Mar Carrió Alexander Lang Gema Revuelta Ana Marušić HEIRRI Consortium | 2021 | Forensic Sciences Research2021，6，3： | 1 |
| 12 | A quasi-Newton acceleration for high-dimensional optimization algorithms显示文摘 | Zhou Hua Alexander D Lange K | | 0，，2： | 1 |
| 13 | Inhibition of flower pigmentation by antisense CHS genes: promoter and minimal sequence requirements for the antisense effect显示文摘 | Alexander R. Krol Leon A. Mur Pieter Lange Joseph N. M. Mol Antoine R. Stuitje | 1990 | Plant Molecular Biology1990，，4： | 1 |
| 14 | Fast model-based estimation of ancestry in unrelated individuals显示文摘 | ALEXANDER D H NOVEMBRE J LANGE K | 2009 | Genome Res2009，19，9： | 1 |
| 15 | Duplex ultrasonography in the diagnosis of incompetent Cockett veins 显示文摘 | Thomas Meyer Alexander Cavallaro Wemer Lang | 2000 | European Journal of Ultrasound2000，11，2000： | 1 |
| 16 | Effects of pantoprazole and esomeprazole on platelet inhibition by clopidogrel显示文摘 | Jolanta M. Siller-Matula Alexander O. Spiel Irene M. Lang Gerhard Kreiner Guenter Christ Bernd Jilma | 2009 | American Heart Journal2009，，1： | 1 |
| 17 | Right Portal Vein Ligation Combined With In Situ Splitting Induces Rapid Left Lateral Liver Lobe Hypertrophy Enabling 2-Staged Extended Right Hepatic Resection in Small-for-Size Settings显示文摘 | Andreas A. Schnitzbauer Sven A. Lang Holger Goessmann Silvio Nadalin Janine Baumgart Stefan A. Farkas Stefan Fichtner-Feigl Thomas Lorf Armin Goralcyk Rüdiger H?rbelt Alexander Kroemer Martin Loss Petra Rümmele Marcus N. Scherer Winfried Padberg Alfred K? | 2012 | Annals of Surgery2012，，3： | 1 |
| 18 | Inhibition of flower pigmentation by antisense CHS genes: promoter and minimal sequence requirements for the antisense effect显示文摘 | Alexander R. Krol Leon A. Mur Pieter Lange Joseph N. M. Mol Antoine R. Stuitje | 1990 | Plant Molecular Biology1990，，4： | 1 |
| 19 | Treatment of Crigler-Najjar type 1 disease: relevance of early liver transplantation显示文摘 | Rolf Schauer Manfred Stangl Thomas Lang Andreas Zimmermann Alexander Chouker Alexander L Gerbes Friedrich W Schildberg Horst G Rau | 2003 | Journal of Pediatric Surgery2003，，8： | 1 |
| 20 | A Curved-Beam Bistable Mechanism显示文摘 | Jin Qiu Jeffrey H Lang Alexander H Slocum | 2004 | Journal of Micro Electro Mechanical Systems2004，13，2： | 1 |
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