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| 1 | The p53-induced lincRNA-p21 derails somatic cell reprogramming by sustaining H3K9me3 and CpG methylation at pluripotency gene promoters显示文摘最近的研究在众多的生物过程增加了我们长 noncoding RNA (lncRNAs ) 的理解,但是很少在体的房间 reprogramming 检验了他们的角色。通过表示介绍并且功能的屏蔽,我们鉴别了大 intergenic noncoding RNA p21 (lincRNA-p21 ) 损害 reprogramming。尤其是, lincRNA-p21 被 p53 导致,但是不在 reprogramming 支持 apoptosis 或房间老朽。相反, lincRNA-p21 与 H3K9 methyltransferase SETDB1 和维护 DNA methyltransferase DNMT1 联系,它被 RNA 有约束力的蛋白质 HNRNPK 便于。因而, lincRNA-p21 由在 pluripotency 基因倡导者支撑 H3K9me3 或 CpG methylation 阻止 reprogramming。我们的结果在 reprogramming 提供卓见进 lncRNAs 的角色并且建立在 p53 和 heterochromatin 规定之间的一个新奇连接。 | Xichen Bao Haitao Wu Xihua Zhu Xiangpeng Guo Andrew P Hutchins Zhiwei Luo Hong Song Yongqiang Chen Keyu Lai Menghui Yin Lingxiao Xu Liang Zhou Jiekai Chen Dongye Wang Baoming Qin Jon Frampton Hung-Fat Tse Duanqing Pei Huating Wang Biliang Zhang Miguel A Esteban | 2015 | Cell Research2015，25，1： | 21 |
| 2 | Effects of ethanol extracts of scorpion on hippocampal apoptosis and caspase-3 expression in lithium chloride-pilocarpine-induced status epilepticus rats显示文摘BACKGROUND: Previous studies have demonstrated that scorpion venom in the scorpion can inhibit epilepsy and apoptosis.However,it remains unclear whether ethanol extracts of scorpion (EES) exhibit similar effects.OBJECTIVE: To investigate the effects of EES on hippocampal apoptosis and caspase-3 expression,and to compare the effects on sodium valproate (positive control drug) in a rat model of status epilepticus induced by lithium chloride-pilocarpine.DESIGN,TIME AND SETTING: This randomized,controlled study was conducted at the Drug Research and Development Center,Kanghong Pharmaceuticals Group,and the Department of Pathology,Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital,China from May 2007 to April 2008.MATERIALS: EES were prepared by Huashen Pharmaceutical,China.Sodium valproate (Hunan Xiangzhong Pharmaceutical,China) and lithium chloride-pilocarpine (Sigma,USA) were also used in the present study.METHODS: From a total of 156 rats,six served as normal controls.The remaining rats were intraperitoneally injected with lithium chloride-pilocarpine to establish status epilepticus models,and then assigned to five groups (n = 30,respectively).Animals in each group were administered drugs at 15 minutes after epileptic seizure by gavage.i.e.in the normal control and model groups,rats were treated with 1 mL/0.1 kg saline.The sodium valproate group was administered 120 mg/kg/d sodium valproate.The low-,moderate-,and high-dose EES groups received treatments of 290,580,and 1 160 mg/kg/d EES.The dispensed concentration was 1 mL/0.1 kg.Rat seizure behavior was observed.If status epilepticus did not terminated after 1 hour,the rats were intraperitoneally administered atropine (1 mg/kg) and diazepam (10 mg/kg) to terminate seizure.These rats were continuously observed for 6 hours to ensure seizure termination.Then rats were treated with the above-mentioned drugs at 8: 00 am each day until sacrifice,which took place 4 hours after drug administration.MAIN OUTCOME MEASURES: Terminal dUTP nick end labeling (TUNEL)-positive cells and caspase-3 expression were,respectively,determined by TUNEL and immunohistochemistry at 6,24,48,and 72 hours,as well as 7 days,after status epilepticus.Behavioral changes were also measured.RESULTS: A few caspase-3-positive cells were observed.TUNEL-and caspase-3-positive cells were mainly visible in the hippocampal CA1 and CA3 regions 6 hours following status epilepticus in the model and drug intervention groups.The number of TUNEL-positive cells reached a peak at 48 hours following status epilepticus in the sodium valproate group,as well as the moderate-and high-dose EES groups,and number of TUNEL-positive cells reached a peak at 72 hours in the model and low-dose EES groups.The number of caspase-3-positive cells reached a peak at 48 hours in each group.Following treatment of sodium valproate and EES,the number of TUNEL-and caspase-3-positive cells significantly decreased compared with the model group at various time points (P < 0.05).The number of TUNEL-and caspase-3-positive cells was greatest in the low-dose EES group,followed by the moderate-and high-dose EES groups.The number of TUNEL-and caspase-3-positive cells was similar between the sodium valproate and high-dose EES groups.Epileptic seizure was significantly improved in the sodium valproate group,as well as the moderate-and high-dose EES groups,compared with the model group (P < 0.05 or P < 0.01).Treatment with sodium valproate and high-dose EES resulted in the best outcome,although the results were similar (P > 0.05).CONCLUSION: A dose of 1 160 mg/kg/d EES significantly inhibited status epilepticus.This outcome corresponded to a decreased number of apoptotic cells and caspase-3-positive cells,which was similar to sodium valproate.These results suggest that it is not necessary to extract a component from the scorpion for the treatment of epilepsy.The high dose of EES significantly inhibited epilepsy,which correlated with decreased hippocampal caspase-3 expression. | Liang Yu Hongbin Sun Yi Liang Yan Xie Baoming He Fei Xu | 2010 | Neural Regeneration Research2010，5，2： | 2 |
| 3 | Scorpion ethanol extract and valproic acid effects on hippocampal glial fibrillary acidic protein expression in a rat model of chronic-kindling epilepsy induced by lithium chloride-pilocarpine显示文摘The present study analyzed the effects of ethanol extracts of scorpion on epilepsy prevention and hippocampal expression of glial fibrillary acidic protein in a lithium chloride-pilocarpine epileptic rat model. Results were subsequently compared with valproic acid. Results showed gradually- increased hippocampal glial fibrillary acidic protein expression following model establishment; glial fibrillary acidic protein mRNA expression was significantly increased at 3 days, reached a peak at 7 days, and then gradually decreased thereafter. Ethanol extracts of scorpion doses of 580 and 1 160 mg/kg, as well as 120 mg/kg valproic acid, led to a decreased number of glial fibrillary acidic protein-positive cells and glial fibrillary acidic protein mRNA expression, as well as decreased seizure grades and frequency of spontaneously recurrent seizures. The effects of 1 160 mg/kg ethanol extracts of scorpion were equal to those of 120 mg/kg valproic acid. These results suggested that the anti-epileptic effect of ethanol extracts of scorpion were associated with decreased hippocampal glial fibrillary acidic protein expression in a rat model of lithium chloride-pilocarpine induced epilepsy. | Yi Liang Hongbin Sun Liang Yu Baoming He Yan Xie | 2012 | Neural Regeneration Research2012，7，6： | 2 |
| 4 | 3D-assembled microneedle ion sensor-based wearable system for the transdermal monitoring of physiological ion fluctuations显示文摘Monitoring human health is of considerable significance in biomedicine.In particular,the ion concentrations in blood are important reference indicators related to many diseases.Microneedle array-based sensors have enabled promising breakthroughs in continuous health monitoring due to their minimally invasive nature.In this study,we developed a microneedle sensing-array integrated system to continuously detect subcutaneous ions to monitor human health status in real time based on a fabrication strategy for assembling planar microneedle sheets to form 3D microneedle arrays.The limitations of preparing 3D microneedle structures with multiple electrode channels were addressed by assembling planar microneedle sheets fabricated via laser micromachining;the challenges of modifying closely spaced microneedle tips into different functionalized types of electrodes were avoided.The microneedle sensing system was sufficiently sensitive for detecting real-time changes in Ca^(2+),K^(+),and Na^(+) concentrations,and it exhibited good detection performance.The in vivo results showed that the ion-sensing microneedle array successfully monitored the fluctuations in Ca^(2+),k^(+),and Na^(+) in the interstitial fluids of rats in real time.By using an integrated circuit design,we constructed the proposed microneedle sensor into a wearable integrated monitoring system.The integrated system could potentially provide information feedback for diseases related to physiological ion changes. | Xinshuo Huang Shantao Zheng Baoming Liang Mengyi He Feifei Wu Jingbo Yang Hui-jiuan Chen Xi Xie | 2023 | Microsystems & Nanoengineering2023，9，2： | 2 |
| 5 | A Mesenchymal-to-Epithelial Transition Initiates and Is Required for the Nuclear Reprogramming of Mouse Fibroblasts显示文摘 | Ronghui Li Jialiang Liang Su Ni Ting Zhou Xiaobing Qing Huapeng Li Wenzhi He Jiekai Chen Feng Li Qiang Zhuang Baoming Qin Jianyong Xu Wen Li Jiayin Yang Yi Gan Dajiang Qin Shipeng Feng Hong Song Dongshan Yang Biliang Zhang Lingwen Zeng Liangxue Lai Miguel | 2010 | Cell Stem Cell2010，，1： | 1 |
| 6 | A Mesenchymal-to-Epithelial Transition Initiates and Is Required for the Nuclear Reprogramming of Mouse Fibroblasts显示文摘 | Ronghui Li Jialiang Liang Su Ni Ting Zhou Xiaobing Qing Huapeng Li Wenzhi He Jiekai Chen Feng Li Qiang Zhuang Baoming Qin Jianyong Xu Wen Li Jiayin Yang Yi Gan Dajiang Qin Shipeng Feng Hong Song Dongshan Yang Biliang Zhang Lingwen Zeng Liangxue Lai Miguel | 2010 | Cell Stem Cell2010，，1： | 1 |
| 7 | Nanoparticles (NPs)-mediated Siglec15 silencing and macrophage repolarization for enhanced cancer immunotherapy显示文摘cell infiltration and proliferation in tumor tissues are the main factors that significantly affect the therapeutic outcomes of cancer immunotherapy.Emerging evidence has shown that interferon-gamma(IFN)could enhance CXCL9 secretion from macrophages to recruit T cells,but Siglec15 expressed on TAMs can attenuate T cell proliferation.Therefore,targeted regulation of macrophage function could be a promising strategy to enhance cancer immunotherapy via concurrently promoting the infiltration and proliferation of T cells in tumor tissues.We herein developed reductionresponsive nanoparticles(NPs)made with poly(disulfide amide)(PDSA)and lipid-poly(ethylene glycol)(lipid-PEG)for systemic delivery of Siglec15 siRNA(siSiglec15)and IFN for enhanced cancer immunotherapy.After intravenous administration,these cargo-loaded could highly accumulate in the tumor tissues and be efficiently internalized by tumor-associated macrophages(TAMs).With the highly concentrated glutathione(GSH)in the cytoplasm to destroy the nanostructure,the loaded IFN and si-Siglec15 could be rapidly released,which could respectively repolarize macrophage phenotype to enhance CXCL9 secretion for T cell infiltration and silence Siglec15 expression to promote T cell proliferation,leading to significant inhibition of hepatocellular carcinoma(HCC)growth when combining with the immune checkpoint inhibitor.The strategy developed herein could be used as an effective tool to enhance cancer immunotherapy. | Xiaodi Liu Qi Zhang Yixia Liang Shiyu Xiong Yan Cai Jincheng Cao Yanni Xu Xiaolin Xu Ye Wu Qiang Lu Xiaoding Xu Baoming Luo | 2023 | Acta Pharmaceutica Sinica B2023，13，12： | 0 |
| 8 | Microarrow sensor array with enhanced skin adhesion for transdermal continuous monitoring of glucose and reactive oxygen species显示文摘Conventional blood sampling for glucose detection is prone to cause pain and fails to continuously record glucose fluctuations in vivo.Continuous glucose monitoring based on implantable electrodes could induce pain and potential tissue inflammation,and the presence of reactive oxygen species(ROS)due to inflammationmay affect glucose detection.Microneedle technology is less invasive,yet microneedle adhesion with skin tissue is limited.In this work,we developed a microarrow sensor array(MASA),which provided enhanced skin surface adhesion and enabled simultaneous detection of glucose and H_(2)O_(2)(representative of ROS)in interstitial fluid in vivo.The microarrows fabricated via laser micromachining were modified with functional coating and integrated into a patch of a three-dimensional(3D)microneedle array.Due to the arrow tip mechanically interlocking with the tissue,the microarrow array could better adhere to the skin surface after penetration into skin.The MASA was demonstrated to provide continuous in vivo monitoring of glucose and H_(2)O_(2) concentrations,with the detection of H_(2)O_(2) providing a valuable reference for assessing the inflammation state.Finally,the MASA was integrated into a monitoring system using custom circuitry.This work provides a promising tool for the stable and reliable monitoring of blood glucose in diabetic patients. | Xinshuo Huang Baoming Liang Shantao Zheng Feifei Wu Mengyi He Shuang Huang Jingbo Yang Qiangqiang Ouyang Fanmao Liu Jing Liu Hui-jiuan Chen Xi Xie | 2024 | Bio-Design and Manufacturing2024，7，1： | 0 |
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