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| 1 | Rapid,visual,and equipment-free point-of-care testing for Staphylococcus aureus by direct recombinase polymerase amplification with SYBR GreenΙ显示文摘Food-borne diseases mainly caused by bacterial pathogens and the safety of genetically modified(GM)food have become global concerns[1].As a leading bacterial pathogen,Staphylococcus aureus can not only cause a variety of diseases[2]but also contaminate different kinds of animal food products[3].In addition,GM foods whose impact on human health and the natural environment is still a matter of controversy have gradually entered the commercial markets[4].Therefore,many countries have decided to implement labeling regulations for authorized genetically modified organisms(GMOs)to protect the consumers’right to know[5].Hence,it is critical to develop a rapid,simple,equipment-free,and on-site testing method to detect the GM contents in food samples and pathogen to ensure food safety. | Xiaorui Fan Fangti Han Binan Zhao Yan Xu Xiao Zhao Xinyi Pu Yanan Du Qi Zhang Xiaoxia Zhang Wanjing Zhang Wenjing Wu Zhiwei Chen Kai Zhao | 2021 | Acta Biochimica et Biophysica Sinica2021，53，9： | 3 |
| 2 | Whole genome engineering by synthesis显示文摘Whole genome engineering is now feasible with the aid of genome editing and synthesis tools. Synthesizing a genome from scratch allows modifications of the genomic structure and function to an extent that was hitherto not possible, which will finally lead to new insights into the basic principles of life and enable valuable applications. With several recent genome synthesis projects as examples, the technical details to synthesize a genome and applications of synthetic genome are addressed in this perspective. A series of ongoing or future synthetic genomics projects, including the different genomes to be synthesized in GPwrite, synthetic minimal genome, massively recoded genome, chimeric genome and synthetic genome with expanded genetic alphabet, are also discussed here with a special focus on theoretical and technical impediments in the design and synthesis process. Synthetic genomics will become a commonplace to engineer pathways and genomes according to arbitrary sets of design principles with the development of high-efficient, low-cost genome synthesis and assembly technologies. | Zhouqing Luo Qing Yang Binan Geng Shuangying Jiang Shihui Yang Xiaozheng Li Yizhi Cai Junbiao Dai | 2018 | Science China(Life Sciences)2018，61，12： | 2 |
| 3 | Graves conséquences du co?t financier du diabète sur sa prise en charge, en dehors des complications, en Afrique sub-saharienne : l’exemple de la C?te-d’Ivoire显示文摘 | A. Ankotche Y Binan A. Leye A.R. Biekre V. Adoueni T. Toutou A. Lokrou | 2009 | Médecine des maladies métaboliques2009，，1： | 1 |
| 4 | Multiplex detection of transgenic maize by microdroplet PCR combined with capillary gel electrophoresis显示文摘With tremendous progress in the commercialization of genetically modified(GM)organisms(GMOs),the safety of GM foods has become a heated topic of debate,and many countries or organizations have adopted the GMO labeling systems[1].GM foods were labeled at 0.9%threshold in the European Union,1%in Brazil,3%in Korea,and 5%in Japan[1,2].In China,all GMOs and GM products are required to be labeled according to the Department of Agriculture,and the main purpose of labeling is to protect consumers’right to know and choose[2]. | Xiao Zhao Qi Zhang Xueming Tang Yan Xu Yanan Du Binan Zhao Xiaori Fan Xiaoxia Zhang Wanjing Zhang Wenjing Wu Kai Zhao | 2019 | Acta Biochimica et Biophysica Sinica2019，51，5： | 1 |
| 5 | Protection of the flavonoid fraction from Rosa laevigata Michx fruit against carbon tetrachloride-induced acute liver injury in mice显示文摘 | Shuai Zhang Binan Lu Xu Han Lina Xu Yan Qi Lianhong Yin Youwei Xu Yanyan Zhao Kexin Liu Jinyong Peng | 2012 | Food and Chemical Toxicology2012，，： | 1 |
| 6 | Approaches for neural tissue regeneration显示文摘 | Binan L Ajji A De Crescenzo G | 2014 | Stem Cell Rev Rep2014，1，： | 1 |
| 7 | Differentiation of neu- ronal stem cells into motor neurons using eleetrospun poly-L-lactic acid/gelatin scaffold 显示文摘 | Binan L Tendey C De Crescenzo G | 2014 | Biomaterials2014，35，2： | 1 |
| 8 | Mechanism investigation of dioscin against CCl 4 -induced acute liver damage in mice显示文摘 | Binan Lu Yousong Xu Lina Xu Xiaonan Cong Lianhong Yin Hua Li Jinyong Peng | 2012 | Environmental Toxicology and Pharmacology2012，，2： | 1 |
| 9 | Simultaneous determination of 11 active components in two well-Kno-wn traditional Chinese medicines by HPLC coupled with diode array detection for quality control显示文摘 | Lianhong Yin Binan Lu Yan Qi | | 0，，： | 1 |
| 10 | Hepatoprotective activity of the total flavonoids from Rosa laevigata Michx fruit in mice treated by paracetamol显示文摘 | LIU Yuetao LU Binan PENG Jinyong | 2011 | Food Chemistry2011，125，2： | 1 |
| 11 | Portulaca oleracea alleviates CCl4-induced acute liver injury by regulating hepatic S100A8 and S100A9显示文摘Objective: Acute liver injury(ALF) is a potential factor of many serious hepatopathies. Carbon tetrachloride(CCl4) is a possible environmental toxicant that can induce ALF. Portulaca oleracea(PO) is one of the most popular edible herbs and has several biological activities such as antioxidant, antimicrobial, antiinflammatory effects. We explored the significance of PO in regulating inflammatory function in animal models and cultured hepatocytes during liver damage caused by CCl4.Methods: The effect of PO on ALF was evaluated by CCl4-induced mice models in vivo. Hepatic levels of transaminase activities and inflammatory factors were examined. The gene and protein expression of S100A8 and S100A9 were measured by RT-PCR and Western blot analysis. Meanwhile, the efficacy of PO was certified by HepG2 cells in vitro. The transaminase activities, inflammatory factors, and the protein expression of S100A8 and S100A9 were also detected.Results: Animal tests showed that pretreatment with PO reduced the liver pathological tissue damage and the serum levels of ALT, AST, ALT and LDH, as well as reducing the pro-inflammatory cytokines(IL-1β, IL-6, TNF-a) secretion in CCl4-induced liver injury mice. Simultaneously, Hep G2 cells pretreated with PO exhibited a significant decrease in the activities of ALT and AST. Moreover, PO resulted in a significant downregulation of the pro-inflammatory markers S100A8, S100A9 gene and protein expression on CCl4induced acute liver injury was demonstrated entirely in vivo and vitro experiments.Conclusion: PO may down-regulate S100A8 and S100A9 and inhibit pro-inflammatory cytokines’ release,indicating a potential clinical effect for controlling the disease. | Aruna Qian Lu Zhou Dongxu Shi Zongran Pang Binan Lu | 2023 | Chinese Herbal Medicines2023，15，1： | 0 |
| 12 | Functional and binding studies of gallic acid showing platelet aggregation inhibitory effect as a thrombin inhibitor显示文摘Objective:This study was devoted to identifying natural thrombin inhibitors from traditional Chinese medicine(TCM)and evaluating its biological activity in vitro and binding characteristics.Methods:A combination strategy containing molecular docking,thrombin inhibition assay,surface plasmon resonance(SPR)and molecular dynamics simulation were applied to verify the study result.Results:Gallic acid was confirmed as a direct thrombin inhibitor with IC;of 9.07μmol/L and showed a significant inhibitory effect on thrombin induced platelet aggregation.SPR-based binding studies demonstrated that gallic acid interacted with thrombin with a KDvalue of 8.29μmol/L.Molecular dynamics and binding free energy analysis revealed that thrombin-gallic acid system attained equilibrium rapidly with very low fluctuations,the calculated binding free energies was-14.61 kcal/mol.Ala230,Glu232,Ser235,Gly258 and Gly260 were the main amino acid residues responsible for thrombin inhibition by gallic acid,providing a mechanistic basis for further optimization.Conclusion:This study proved that gallic acid is a direct thrombin inhibitor with platelet aggregation inhibitory effect,which could provide a basis for the follow-up research and development for novel thrombin inhibitors. | Yuxin Zhang Xing Wang Binan Lu Yanbin Gao Yanling Zhang Yatong Li Hongjuan Niu Lu Fan Zongran Pang Yanjiang Qiao | 2022 | Chinese Herbal Medicines2022，14，2： | 0 |
| 13 | Identification and characterization of related substances in EVT-401 by hyphenated LC–MS techniques显示文摘A sensitive and selective method was developed for the separation and characterization of related substances(RSs) in EVT-401 by hyphenated LC–MS techniques. Complete separation of the RSs was achieved with an Inertsil ODS-SP column(250 mm×4.6 mm, 5 μm) by linear gradient elution using a mobile phase consisting of 0.2% formic acid solution, methanol and acetonitrile. EVT-401 was found to be susceptible to acid, alkaline and oxidative stresses, while relatively stable under photolytic and thermal dry stress conditions. Fourteen RSs including six process-related substances and eight degradation products were detected and identified in EVT-401 with positive ESI high-resolution TOF-MS analysis of their parent ions and the corresponding product mass spectra elucidation, and some of them were further verified by chemical synthesis and NMR spectroscopy. The specific LC–MS method developed for separation, identification and characterization of RSs is valuable for EVT-401 manufacturing process optimization and quality control. | Binan Zhu Yuting Lu Leilin Chen Binbin Yu Yuexin Liu Min Song Taijun Hang | 2017 | Journal of Pharmaceutical Analysis2017，7，4： | 0 |
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