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| 1 | Type 1 regulatory T cells: a new mechanism of periphera immune tolerance显示文摘对抗原的有免疫力的反应的缺乏,作为有免疫力的忍耐知道的一个过程,为有免疫力的动态平衡的保藏是必要的。迄今为止,驾驶有免疫力的忍耐的二机制广泛地被描述了:中央忍耐和外部忍耐。在新名称下面,导出胸腺的规章的 T (tT reg ) 房间是中央有免疫力的忍耐的主要调停人,而外围地发源规章的 T (磅 reg ) 房间工作调整外部有免疫力的忍耐。T reg 房间,称为它 reg , 仅仅代表在里面导致 vitro 的 T reg 房间 1 。取决于房间是否稳定地表示 Foxp3,磅 reg , 和它 reg 房间可以被划分成二个子集:古典 CD4 + Foxp3 + T reg 房间和 CD4 + Foxp3 − 类型 1 规章的 T (Tr1 ) 房间 2 。这评论集中于发现,联系 biomarkers,规章的功能,正式就职的方法,有疾病的协会,和 Tr1 房间的临床的试用。 | Hanyu Zeng Rong Zhang Boquan Jin Lihua Chen | 2015 | Cellular & Molecular Immunology2015，12，5： | 19 |
| 2 | A novel interface consisting of homologous immunoglobulin superfamily members with multiple functions显示文摘Immunoglobulin superfamily(IgSF)members account for a large proportion of cell adhesion molecules that perform important immunological functions,including recognizing a variety of counterpart molecules on the cell surface or extracellular matrix.The findings that CD155/poliovirus receptor(PVR)and CD112/nectin-2 are the ligands for CD226/platelet and T-cell activation antigen 1(PTA1)/DNAX accessory molecular-1(DNAM-1),CD96/tactile and Washington University cell adhesion molecule(WUCAM)and that CD226 is physically and functionally associated with lymphocyte function-associated antigen-1(LFA-1)on natural killer(NK)and activated T cells have largely expanded our knowledge about the functions of CD226,CD96,WUCAM and LFA-1 and their respective ligands,CD155,CD112,intercellular adhesion molecule(ICAM)-1 and junctional adhesion molecule(JAM)-1.The interactions of these receptors and their ligands are involved in many key functions of immune cells including naive T cells,cytotoxic T cells,NK cells,NK T cells,monocytes,dendritic cells,mast cells and platelets/megakaryocytes. | Zhuwei Xu Boquan Jin | 2010 | Cellular & Molecular Immunology2010，7，1： | 8 |
| 3 | Characterization of the neutralizing activity of three anti-human TNF monoclonal antibodies and prediction of their TNF epitopes by molecular modeling and mutant protein approach显示文摘 | Cansheng Zhu Xuesong Liu Jiannan Feng Wei Zhang Beifen Shen Weiming Ou'yang Yunxin Cao Boquan Jin | 2006 | 中国生物学文摘2006，20，1： | 5 |
| 4 | Sema 4D/CDlOO-plexin B is a multifunctiona counter-receptor显示文摘 | Yusi Zhang Bei Liu Ying Ma Boquan Jin | 2013 | Cellular & Molecular Immunology2013，10，2： | 3 |
| 5 | Enzyme-linked immunosorbent assay of changes in serum levels of growth hormone(cGH)in common carps(Cyprinus carpio)显示文摘The aim of the present study was to purify the common native carp growth hormone (ncGH), produce monoclonal antibodies (mAbs) to common native carp growth hormone (ncGH), and further enhance the sensitivity of enzyme-linked immunosorbent assays (ELISA) for ncGH. Additionally, we investigated changes in serum ncGH levels in carps raised in different environmental conditions. The recombinant grass carp (Ctenopharyngodon idella) growth hormone was purified and used as antigen to immunize the rabbit. The natural ncGH was isolated from the pituitaries of common carp. SDS-PAGE and Western blot utilizing the polyclonal anti-rgcGH antibody confirmed the purification of ncGH from pituitaries. Purified ncGH was then used as an immunogen in the B lymphocyte hybridoma technique. A total of 14 hybridoma cell lines (FMU-cGH 1-14) were established that were able to stably secrete mAbs against ncGH. Among them, eight clones (FMU-cGH1-6, 12 and 13) were successfully used for Western blot while nine clones (FMU-cGH 1-7, 9 and 10) were used in fluorescent staining and immunohistochemistry. Epitope mapping by competitive ELISA demonstrated that these mAbs recognized five different epitopes. A sensitive sandwich ELISA for detection of ncGH was developed using FMU-cGH12 as the coating mAb and FMU-cGH6 as the enzyme labeled mAb. This detection system was found to be highly stable and sensitive, with detection levels of 70 pg/mL. Additionally, we found that serum ncGH levels in restricted food group and in the net cage group increased 6.9-and 5.8-fold, respectively, when compared to controls, demonstrating differences in the GH stress response in common carp under different living conditions. | WU Gang CHEN LiHua ZHONG Shan LI Qi SONG ChaoJun JIN BoQuan ZHU ZuoYan | 2008 | Science China(Life Sciences)2008，51，2： | 1 |
| 6 | Thrombopoietin induced proliferation and differentiation of fetal liver CD34^+ cells with phenotype change from hemopoiesis to neurogenesis显示文摘BACKGROUND: Previous studies have reported a neurotrophin-like motif in the N-terminal receptor binding region of the thrombopoietin (TPO) molecule, and have described localization of TPO and TPO receptor in the brain. Therefore, it is believed that TPO may be involved in regulation of neurogenesis. OBJECTIVE: To validate the effect of TPO on trans-differentiation, or differentiation from hematopoietic stem cells (HSCs) to neural stem cells (NSCs). DESIGN, TIME AND SETTING: Comparative studies were performed from March 2004 to April 2007 at the Department of Experimental Medicine, Northern Hospital, and the Department of Immunology, Fourth Military Medical University of Chinese PLA. MATERIALS: Human fetal liver (FL) was obtained from fetuses after water-balloon abortion. Gestational age ranged from 16 to 20 weeks. The study was approved by the Institutional Review Board and Ethics Committee of the Northern Hospital. TPO was kindly provided by Genentech Inc (USA). Iscove's Modified Dulbecco's Medium (IMDM) and neurobasalTM medium were purchased from Invitrogen (USA). MACS CD34 multisort kit was purchased from Miltenyi Biotec (Germany). METHODS: CD34+ cells were isolated from human FL mononuclear cells using MACS CD34 multisort kit and cultured at 1 × 105/mL in IMDM, containing TPO for 60 days with weekly changes of half of the medium. After culturing for 30 and 60 days, the TPO-induced cells were resuspended in neurobasalTM medium containing 10% fetal brain extracts and plated in an 8-well BIOCOAT poly-D-Lysine Culture Slide and cul- tured for another 7 days. MAIN OUTCOME MEASURES: Cell number, viability, phenotype and expression of hemopoiesis-related and neurogenesis-related proteins were examined by trypan blue exclusion with hemocytometer, immunoblot, immunocytochemistry and flow cytometry. RESULTS: After 60 days of induction with TPO, the cell number increased by 4.6-fold compared to the ini- tial culture. Although the proportion of the cells expressing the hemopoietic stem cell associated antigen (CD34) decreased steadily, both proportions of the cultured FL-derived CD34+ cells expressing CD41a and CD61 remained unchanged, which still accounted for 10%. Noticeably, the proportions of the cells express- ing nestin and epidermal growth factor receptor increased significantly (both > 50%), whereas the expression of more mature neural or glial proteins [microtubule-associated protein-2 (MAP2), glial fibrillary acidic pro- tein (GFAP), oligodendrocyte marker O4 (O4)] markers on the cultured fetal liver derived-CD34+ cells were at lower levels. After another 7 days incubation in neurobasal? medium, these TPO-induced cells formed neurospheres, which were labeled with nestin, and differentiated into cells with morphological characteristics of neurons, astrocytes and oligodendrocytes, which were labeled with MAP-2, GFAP, and O4, respectively. CONCLUSION: TPO can induce FL-derived HSCs to differentiate or trans-differentiate into NSCs and its progenitors. | Ning Ma Dongchu Ma Yi Tao Yinghui Sun Di Lin Huiying Yu Jinlong Jian Wei Jia Boquan Jin | 2008 | Neural Regeneration Research2008，3，4： | 1 |
| 7 | Postt- ranslational modifications of P27^kipl determine its binding specificity to different cyclins and cyclin-dependent kinases in vivo 显示文摘 | Zhang Wenqing Bergamaschi D Jin Boquan | 2005 | Blood2005，105，： | 1 |
| 8 | The transcription co-repressor TLE1 interacted with the intracellular region of gp130 through its Q domain显示文摘 | Fei Liu Yin Liu Demin Li Yong Zhu Weiming Ouyang Xin Xie Boquan Jin | 2002 | Molecular and Cellular Biochemistry (-)2002，，1： | 1 |
| 9 | A new method specified for detecting apoptosis and cell cycles显示文摘 | CAO YUNXIN JIN WEILIN JIN BOQUAN | 2003 | Chinese J Med Res2003，12，： | 1 |
| 10 | Establishment of an ELISA system for determining soluble LAIR-1 levels in sera of patients with HFRS and kidney transplant显示文摘 | Weiming Ouyang Jiangnan Xue Jingmei Liu Wei Jia Zhouli Li Xin Xie Xuesong Liu Jinlong Jian Qi Li Yong Zhu Angang Yang Boquan Jin | 2004 | Journal of Immunological Methods2004，，： | 1 |
| 11 | HLA-E-restricted Hantaan virus-specific CD8^(+)T cell responses enhance the control of infection in hemorrhagic fever with renal syndrome显示文摘Infection with the Hantaan virus(HTNV)may result in severe hemorrhagic fever with renal syndrome(HFRS).The functions of HLA-E-restricted CD8^(+)T lymphocytes in virus control and vaccine development have recently received increased attention.The purpose of this research is to discover HLA-E-restricted CD8^(+)T cell epitopes on HTNV as well as the features of these epitope-specific CD8^(+)T cells in HFRS patients.To anticipate HLA-Erestricted HTNV epitopes,the NetMHCpan servers were utilized.The K562/HLA-E cell binding test and the enzyme-linked immunospot assay were used to confirm epitope binding to HLA-E.The number and features of HLA-E-restricted epitope-specific CD8^(+)T lymphocytes in HFRS patients were investigated using tetramer staining,intracellular cytokine labeling,proliferation,and cytotoxicity assays.Six HTNV-derived HLA-Erestricted CD8^(+)T cell epitopes were found in this study.In mild/moderate HFRS patients,the frequency of HLA-E-restricted epitope-specific CD8^(+)T cells was greater than in severe/critical patients.CD38+HLA-DR+HLA-E-restricted CD8^(+)T cells were identified.Meanwhile,CD45RA^(+)CCR7^(-)effector memory-re-expressing CD45RA T cells with early and intermediate maturation and differentiation characteristics predominated.Notably,CD8^(+)T cells from milder HFRS patients produced more interferon-γ,interleukin-2,and granzyme B,had a stronger proliferative potential,and were inversely linked with the amount of plasma HTNV virus load.Furthermore,HLA-E-restricted epitope-specific CD8^(+)T cells demonstrated improved cytotoxic activity in vitro during the acute stage of HFRS.Taken together,the findings demonstrate the protective effects of HLA-E-restricted CD8^(+)T cells during HTNV infection,suggesting that HLA-E-targeted vaccines against HTNV might be developed for HLA-diverse populations. | Kang Tang Yusi Zhang Xinyu Li Chunmei Zhang Xiaozhou Jia Haifeng Hu Lihua Chen Ran Zhuang Yun Zhang Boquan Jin Ying Ma | 2023 | Biosafety and Health2023，5，5： | 0 |
| 12 | Erratum to'HLA-E-restricted Hantaan virus-specific CD8^(+)T cell responses enhance the control of infection in hemorrhagic fever with renal syndrome'[Biosaf.Health 5(2023)289-299]显示文摘The publisher regrets that during proofreading of the figure legend of Fig.6,the P value was misspelled in the sentence'while'n.s.'indicates no significant difference P<0.05'.It should be corrected to''while'n.s.'indicates no significant difference(P>0.05)'. | Kang Tang Yusi Zhang Xinyu Li Chunmei Zhang Xiaozhou Jia Haifeng Hu Lihua Chen Ran Zhuang Yun Zhang Boquan Jin Ying Ma | 2024 | Biosafety and Health2024，6，1： | 0 |
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