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| 1 | Highly efficient heritable genome editing in wheat using an RNA virus and bypassing tissue culture.显示文摘Genome editing provides novel strategies for improving plant traits,but relies on current genetic transformation and plant regeneration procedures,which can be inefficient.We have engineered a barley stripe mosaic virus(BSMV)-based sgRNA delivery vector(BSMV-sg)that is effective in performing heritable genome editing in Cas9-transgenic wheat plants.Mutated progenies were present in the next generation at frequencies ranging from 12.9%to 100%in three different wheat varieties,and 53.8%to 100%of mutants were virus-free.We also achieved multiplex mutagenesis in progeny using a pool of BSMV-sg vectors harboring different sgRNAs.Furthermore,we devised a virus-induced transgene-free editing procedure(VITF-Edit)to generate Cas9-free wheat mutants by crossing BSMV-infected Cas9-transgenic wheat pollen with wild-type wheat.Our study provides a robust,convenient and tissue culture-free approach for genome editing in wheat through virus infection. | Tingdong Li Jiacheng Hu Yu Sun Boshu Li Dingliang Zhang Wenli Li Jinxing Liu Dawei Li Caixia Gao Yongliang Zhang Yanpeng Wang | 2021 | Molecular Plant2021，14，11： | 10 |
| 2 | General competencies of problem - based learning (PBL)and non - PBL graduates显示文摘 | Prince KJ van Eijs PW Boshu izen HP | 2005 | Med Educ2005，39，4： | 1 |
| 3 | Application of a low pressure economizer for wasteheat recovery from the exhaust flue gas in a 600 MW power plant显示文摘 | Chaojun Wang Boshu He Shaoyang Sun | 2012 | Energy2012，20，： | 1 |
| 4 | Temperature impact on SO2 removal efficiency by ammonia gas scrubbing显示文摘 | He Boshu Zheng Xianyu Wen Yah | 2003 | Energy Conversion and Management2003，44，13： | 1 |
| 5 | Experimental study on capturing CO2 greenhouse gas by ammonia scrubbing显示文摘 | DIAO YONGFA ZHENG XIANYU HE BOSHU | 2004 | Energy Conversion and Management2004，45，： | 1 |
| 6 | Application of a low pressure economizer for waste heat recovery from the exhaust flue gas in a 600 MW power plant显示文摘 | Chaojun Wang Boshu He Shaoyang Sun Ying Wu Na Yan Linbo Yan Xiaohui Pei | 2012 | Energy2012，，1： | 1 |
| 7 | Numerical study of the optimum counter-flow mode of air jets in a large utility furnace显示文摘 | HE BOSHU CHEN MEIQIAN YU QIUMEI | 2004 | Computers and fluids2004，33，： | 1 |
| 8 | Temperature impact on SO2 removal efficiency by ammonia gas scrubbing显示文摘 | He Boshu Zheng Xianyu Wen Yan | 2003 | Energy Conversion and Management2003，44，13： | 1 |
| 9 | General competencies of prob-lem-based learning (PBL) and non-PBL graduates显示文摘 | Prince KJ van Eijs PW Boshu izen HP | 2005 | Med Educ2005，39，4： | 1 |
| 10 | Kinetic models for coal hydrogasification and analyses of hydrogasification characteristics in entrained-flow gasifiers 显示文摘 | YAN Linbo HE Boshu PEI Xiaohui | 2013 | Energy & Fuels2013，27，11： | 1 |
| 11 | Computational-fluid-dynamics-based evaluation and optimization of an entrained-flow gasifier potential for coal hydrogasification 显示文摘 | YAN Linbo HE Boshu PEI Xiaohui | 2013 | Energy & Fuels2013，27，11： | 1 |
| 12 | Energy ecological efficiency of coal fired plant in China显示文摘 | Boshu He Changhe Chen | | 0，，： | 1 |
| 13 | Numerical study of the optimum counter-flow mode of air jets in a large utility furnace显示文摘 | HE Boshu CHEN Meiqian YU Qiumei LIU Shumin FAN Lijuan SUN Shouguang XU Jinyuan PAN Wei-Ping | | 0，，： | 1 |
| 14 | Application of a low pressure economizer for waste heat recovery from the exhaust flue gas in a 600 MW power plant显示文摘 | Chaojun Wang Boshu He Shaoyang Sun Ying Wu Na Yan Linbo Yan Xiaohui Pei | 2012 | Energy2012，，1： | 1 |
| 15 | Thermal characteristics and thermal stress analysis of solar thermochemical reactor under high-flux concentrated solar irradiation显示文摘Nowadays, using a solar-driven thermochemical reaction system to convert greenhouse gases into high-quality liquid fuels has been proven to be an effective way to address the growing depletion of traditional fossil fuels. However, the utilization of highlyconcentrated solar irradiation runs the high risk of reactor damage issues resulting from thermal stress concentration, which seriously threatens the security and reliability of the total reactor system. In this study, the thermal radiation distribution and thermo-mechanical process in a volumetric reactor were numerically investigated by combining Monte Carlo ray-tracing method with computational fluid dynamics method. Based on the experimental results and thermal characteristic analysis, the formation mechanism of thermal stress concentration and the strategies of improving thermal stress distribution were discussed in detail.The simulation results indicate a great possibility of reactor damage at about 1000℃ operating temperature and 9.0 k W lamp power, which is well-matched with related experimental results. The ceramic damage typically occurs at the inner edges of the through-holes, including the aperture, the gas inlet, and the thermocouple hole, then extends along the lines connecting these holes and finally causes brittle fracture. By reasonable control of the opening direction and the distance between the throughholes, the maximum compressive stress can be reduced by 21.78%. | ZHANG Hao SHUAI Yong GUENE LOUGOU Bachirou JIANG BoShu HUANG Xing | 2020 | Science China(Technological Sciences)2020，63，9： | 1 |
| 16 | Experimental study on capturing CO2 greenhouse gas by ammonia scrubbing显示文摘 | Diao Yongfa Zheng Xianyu He Boshu | 2004 | Energy Conversion and Management2004，，45： | 1 |
| 17 | Temperature impact on SO 2 removal efficiency by ammonia gas scrubbing显示文摘 | Boshu He Xianyu Zheng Yan Wen Huiling Tong Meiqian Chen Changhe Chen | 2002 | Energy Conversion and Management2002，，13： | 1 |
| 18 | Measured vorticity distributions in a model of tangentially fired furnace 显示文摘 | Boshu He Jinyuan Xu | 2005 | Experimental Thermal and Fluid Science2005，15，5： | 1 |
| 19 | Thermodynamic analysis of a low-pressure economizer based waste heat recovery system for a coal-fired power plant显示文摘 | Chaojun Wang Boshu He Linbo Yan Xiaohui Pei Shinan Chen | 2013 | Energy2013，，： | 1 |
| 20 | Application of a low pressure economizer for waste heat recovery from the exhaust flue gasin a 600MW power plant 显示文摘 | Chaojun Wang Boshu He Shaoyang Sun | 2012 | Energy2012，48，1： | 1 |
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