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| 1 | Mobius Isoparametric Hypersurfaces in S^(n+1) with Two Distinct Principal Curvatures | Hai Zhong LI Department of Mathematics, Tsinghua University. Beijing 100084. P. R. China Hui Li LIU Department of Mathematics, Northeastern University. Shenyang 110000. P. R. China Chang Ping WANG Key Laboratory of Pure and Applied Mathematics, School of Mathematical Sciences. Peking University, Beijing 100871, P. R. China Guo Song ZHAO Department of Mathematics, Sichuan University, Chengdu 610064. P. R. China | 2002 | Acta Mathematica Sinica,English Series2002，18，3： | 53 |
| 2 | The Role of Fibrocyte in the Pathogenesis of Silicosis显示文摘Exposure to free silica induces silicosis and myofibroblasts are regarded as primary effector cells.Fibrocytes can differentiate into myofibroblast.Therefore,the present study was designed to investigate whether | LI Juan YAO Wu HOU Jian Yong ZHANG Lin BAO Lei CHEN Hui Ting WANG Di YUE Zhong Zheng LI Yi Ping ZHANG Miao YU Xing Hao ZHANG Jian Hui QU Ya Qian HAO Chang Fu | 2018 | Biomedical and Environmental Sciences2018，31，4： | 20 |
| 3 | Wet and dry deposition fluxes of heavy metals in Pearl River Delta Region(China):Characteristics,ecological risk assessment,and source apportionment显示文摘The atmospheric deposition of heavy metals poses serious risks to the ecological system and human health. To advance our knowledge of atmospheric dry/wet heavy metal deposition in the PRD region, monthly fluxes were examined based on soluble/insoluble fractions of five heavy metal elements(Cu, Pb, Cd, Cr and Zn) in samples collected from January 2014 to December 2015 at Guangzhou(urban) and Dinghushan(suburban) sites. The ratios of wet/dry deposition fluxes indicated that heavy metal deposition was governed by wet deposition rather than dry deposition in the PRD region. Affected by the shifting of the Asian monsoon, wet deposition fluxes exhibited significant seasonal variation between summer monsoon seasons(April to September) and winter monsoon seasons(October to February) in this region. Cd was classified as an extremely strong potential ecological risk based on solubility and the Hakanson ecological risk index. Source contributions to wet deposition were calculated by PMF, suggesting that dust, biomass burning, industries,vehicles, long-range transport and marine aerosol sources in Guangzhou, and Zn fertilizers,marine aerosol sources, agriculture, incense burning, biomass burning, vehicles and the ceramics industry in Dinghushan, were the potential sources of heavy metals. | Lyumeng Ye Minjuan Huang Buqing Zhong Xuemei Wang Qiulan Tu Haoran Sun Chao Wang Luolin Wu Ming Chang | 2018 | Journal of Environmental Sciences2018，30，8： | 20 |
| 4 | Outcomes of endoscopic sphincterotomy vs open choledochotomy for common bile duct stones显示文摘BACKGROUND Endoscopic sphincterotomy(EST) for the management of common bile duct stones(CBDS) is used increasingly widely because it is a minimally invasive procedure. However, some clinical practitioners argued that EST may be complicated by post-endoscopic retrograde cholangiopancreatography(ERCP)pancreatitis(PEP) and accompanied by a higher recurrence of CBDS than open choledochotomy(OCT). Whether any differences in outcomes exist between these two approaches for treating CBDS has not been thoroughly elucidated to date.AIM To compare the outcomes of EST vs OCT for the management of CBDS and to clarify the risk factors associated with stone recurrence.METHODS Patients who underwent EST or OCT for CBDS between January 2010 and December 2012 were enrolled in this retrospective study. Follow-up data were obtained through telephone or by searching the medical records. Statistical analysis was carried out for 302 patients who had a follow-up period of at least 5 years or had a recurrence. Propensity score matching(1:1) was performed to adjust for clinical differences. A logistic regression model was used to identify potential risk factors for recurrence, and a receiver operating characteristic(ROC)curve was generated for qualifying independent risk factors.RESULTS In total, 302 patients undergoing successful EST(n = 168) or OCT(n = 134) were enrolled in the study and were followed for a median of 6.3 years. After propensity score matching, 176 patients remained, and all covariates were balanced. EST was associated with significantly shorter time to relieving biliary obstruction, anesthetic duration, procedure time, and hospital stay than OCT(P <0.001). The number of complete stone clearance sessions increased significantly in the EST group(P = 0.009). The overall incidence of complications and mortality did not differ significantly between the two groups. Recurrent CBDS occurred in18.8%(33/176) of the patients overall, but no difference was found between the EST(20.5%, 18/88) and OCT(17.0%, 15/88) groups. Factors associated with CBDS recurrence included common bile duct(CBD) diameter > 15 mm(OR =2.72; 95%CI: 1.26-5.87; P = 0.011), multiple CBDS(OR = 5.09; 95%CI: 2.58-10.07; P< 0.001), and distal CBD angle ≤ 145°(OR = 2.92; 95%CI: 1.54-5.55; P = 0.001). The prediction model incorporating these factors demonstrated an area under the receiver operating characteristic curve of 0.81(95%CI: 0.76-0.87).CONCLUSION EST is superior to OCT with regard to time to biliary obstruction relief, anesthetic duration, procedure time, and hospital stay and is not associated with an increased recurrence rate or mortality compared with OCT in the management of CBDS. | Xiao-Dong Zhou Qiao-Feng Chen Yuan-Yuan Zhang Ming-Ju Yu Chang Zhong Zhi-Jian Liu Guo-Hua Li Xiao-Jiang Zhou Jun-Bo Hong You-Xiang Chen | 2019 | World Journal of Gastroenterology2019，25，4： | 17 |
| 5 | A 3D hybrid grid generation technique and a multigrid/parallel algorithm based on anisotropic agglomeration approach显示文摘A hybrid grid generation technique and a multigrid/parallel algorithm are presented in this paper for turbulence flow simulations over three-dimensional (3D) complex geometries. The hybrid grid generation technique is based on an agglomeration method of anisotropic tetrahedrons. Firstly, the complex computational domain is covered by pure tetrahedral grids, in which anisotropic tetrahedrons are adopted to discrete the boundary layer and isotropic tetrahedrons in the outer field. Then, the anisotropic tetrahedrons in the boundary layer are agglomerated to generate prismatic grids. The agglomeration method can improve the grid quality in boundary layer and reduce the grid quantity to enhance the numerical accuracy and efficiency. In order to accelerate the convergence history, a multigrid/parallel algorithm is developed also based on anisotropic agglomeration approach. The numerical results demonstrate the excellent accelerating capability of this multigrid method. | Zhang Laiping Zhao Zhong Chang Xinghua He Xin | 2013 | Chinese Journal of Aeronautics2013，26，1： | 14 |
| 6 | Molecular Typing of Brucella Suis Collected from 1960s to 2010s in China by MLVA and PFGE显示文摘Brucellosis is a bacterial anthropozoonosis usually caused by Brucella abortus, Brucella melitensis, Brucella suis and Brucella canis. Brucella suis, the causative agent of swine brucellosis, is classified into five biovars and preferentially infects different animal hosts [1] . | LI Zhen Jun CUI Bu Yun CHEN Hai CHEN Jing Diao ZHAO Hong Yan PIAO Dong Ri JIANG Hai ZHANG Li TANG Xu KE Chang Wen YAO zhen TIAN Guo Zhong | 2013 | Biomedical and Environmental Sciences2013，26，6： | 13 |
| 7 | nterferon alpha (IFNα)-induced TRIM22 interrupts HCV 'eplication by ubiquitinating NS5A显示文摘 | Chen Yang Xinhao Zhao Dakang Sun Leilei Yang Chang Chong Yu Pan Xiumei Chi Yanhang Gao Moli Wang Xiaodong Shi Haibo Sun Juan Lv Yuanda Gao Jin Zhong Junqi Niu Bing Sun | 2016 | Cellular & Molecular Immunology2016，13，1： | 13 |
| 8 | Numerical investigation on flow and convective heat transfer of aviation kerosene at supercritical conditions显示文摘In this paper,characteristics of flow and convective heat transfer of China RP-3 kerosene in straight circular pipe were numerically studied.Navier-Stokes equations were solved using RNG k-turbulence model with low Reynolds number correction.The thermophysical and transport properties of the China RP-3 kerosene were calculated with a 10-species surrogate and the extended corresponding state method(ECS) combined with Benedict-Webb-Rubin equation.The independence of grids was first studied and the numerical results were then compared with experimental data for validation.Under flow conditions given in the paper,the results show that deterioration of convective heat transfer occurs when the wall temperature is slightly higher than the pseudo-critical temperature of kerosene for cases with wall heat flux of 1.2 and 0.8 MW/m 2.The degree of the heat transfer deterioration is weakened as the heat flux decreases.The deterioration,however,does not happen when the heat flux on the pipe wall is reduced to 0.5 MW/m 2.Based on the analysis of the near-wall turbulent properties,it is found that the heat transfer deterioration and then the enhancement are attributed partly to the change in the turbulent kinetic energy in the vicinity of pipe wall.The conventional heat transfer relations such as Sieder-Tate and Gnielinski formulas can be used for the estimation of kerosene heat convection under subcritical conditions,but they are not capable of predicting the phenomenon of heat transfer deterioration.The modified Bae-Kim formula can describe the heat transfer deterioration.In addition,the frictional drag would increase dramatically when the fuel transforms to the supercritical state. | DANG GuoXin ZHONG FengQuan CHEN LiHong CHANG XinYu | 2013 | Science China(Technological Sciences)2013，56，2： | 12 |
| 9 | The OsAMT1.1 gene functions in ammonium uptake and ammoniumepotassium homeostasis over low and high ammonium concentration ranges显示文摘Rice(Oryza sativa) grown in paddy fields is an ammonium(NH^+_4)-preferring crop;however,its AMT-type NH^+_4transporters that mediate root N acquisition have not been well characterized yet.In this study,we analyzed the expression pattern and physiological function of the OsAMT1.1 gene of the AMT1 subfamily in rice.Os AMT1.1 is located in the plasma membrane and is mainly expressed in the root epidermis,stele and mesophyll cells.Disruption of the Os AMT1.1 gene decreased the uptake of NH^+_4,and the growth of roots and shoots under both low NH^+_4and high NH^+_4conditions.Os AMT1.1 contributed to the short-term(5 min)^(15)NH^+_4influx rate by approximately one-quarter,irrespective of the NH^+_4concentration.Knockout of Os AMT1.1 significantly decreased the total N transport from roots to shoots under low NH^+_4conditions.Moreover,compared with the wild type,the osamt1.1 mutant showed an increase in the potassium(K)absorption rate under high NH^+_4conditions and a decrease under low NH^+_4conditions.The mutants contained a significantly high concentration of K in both the roots and shoots at a limited K(0.1 mmol/L)supply when NH^+_4was replete.Taken together,the results indicated that OsAMT1.1 significantly contributes to the NH^+_4uptake under both low and high NH^+_4conditions and plays an important role in Ne K homeostasis in rice. | Chang Li Zhong Tang Jia Wei Hongye Qu Yanjie Xie Guohua Xu | 2016 | Journal of Genetics and Genomics2016，43，11： | 12 |
| 10 | A 12-year cohort study on the efficacy of plasmaderived hepatitis B vaccine in rural newborns显示文摘AIM To understand the anti-HBs persistenceand the long-term preventive efficacy in ruralnewborns after vaccination with plasma-derivedhepatitis B vaccine.METHODS In the time of expanded program onimmunization(EPI),the newborns werevaccinated with 10μg×3 doses of hepatitis Bvaccine and 762 newborns who were HBsAgnegative after primary immunization wereselected for cohort observation from 1986 to1998.Their serum samples were detectedqualitatively and quantitatively for hepatitis Binfecting markers,including HBsAg,anti-HBsand anti-HBc by SPRIA Kits.The annual HBsAgpositive conversion rate was counted by life-table method.RESULTS①The anti-HBs positive rate was94.44% for the babies born to HBsAg negativemothers and 84.21% for those born to HBsAgpositive mothers in the 1st year afterimmunization,and dropped to 51.31% and52.50% in the 12th year respectively.GMT valuewas dropped from 31.62 to 3.13 and 23.99 to 3.65in the 2nd to the 12th year respectively.Therewas a marked drop in GMT at the 3rd to the 5thyear,and in anti-HBs positive rate at the 9th tothe 10th year.②In the period of 12 yearsobservation,the person-year HBsAg positive conversion rates were 0.12%(5/4150.0)innewborns born to HBsAg negative mothers and0.20%(1/508.0)in those born to HBsAgpositive mothers,and none of the HBsAgpositive converted children became HBsAgchronic carriers.Compared with the baselinebefore immunization,the protective rates were97.19% and 95.32% respectively.CONCLUSION The protective efficacy ofplasma-derived hepatitis B vaccine persisted atleast 12 years,and a booster dose seems notnecessary within at least 12 years after theprimary three-doses immunization to newbornsborn to HBsAg negative mothers. | Hong Bin Liu Zong Da Meng Jing Chen Ma Chang Quan Han Ying Lin Zhang Zhan Chun Xing Yu Wei Zhang Yu Zhong Liu Hui Lin Cao | 2000 | World Journal of Gastroenterology2000，6，3： | 11 |
| 11 | Comparative proteogenomic analysis of the Leptospira interrogans virulence-attenuated strain IPAV against the pathogenic strain 56601显示文摘稀释毒力的螺旋体 interrogans serovar Lai 紧张 IPAV 被延长实验室经过从在中国孤立的高度剧毒的祖先的紧张导出。我们学习了 IPAV 的基因变化对病原的 L 经由比较分析使它变为无毒害。interrogans serovar Lai 紧张 56601。IPAV 紧张的完全的染色体顺序决心、使用与作比较,然后修正并且重新注解紧张 56601 的染色体顺序。除了他们的高度类似的 genomic,结构和基因顺序, 33 插入的一个总数, 53 删除和 301 个单个核苷酸的变化(SNV ) 在整个直接影响 101 基因的 IPAV 的染色体被检测,在他们的 5 ′ 的任何一个;在上游的区域或在他们的编码区域以内。在他们之中, 44 功能的基因的多数涉及信号 transduction,压力反应, transmembrane 运输和氮新陈代谢。基于量的液体,层析(LC )-MS/MS 数据揭示了的比较 proteomic 分析在 orthologs 的 1 627 选择的对之中,在 IPAV 的 174 基因拉紧,这是 upregulated,与主要在精力的班上的丰富生产和类脂化合物新陈代谢。相反,在紧张 56601 的 228 基因是 upregulated,与在蛋白质翻译和 DNA 复制 / 修理的范畴充实的多数。 genomic 和 proteomic 途径的联合在批评基因说明了那改变的表情或变化,例如编码 Ser/Thr kinase ,碳饥饿蛋白质 CstA ,夫酸安合成酶,GTP有约束力的蛋白质 BipA , ribonucleotide-diphosphate reductase 和磷酸盐 transporter 的那些,并且在脂蛋白或外部膜蛋白质的翻译侧面的改变是可能的在紧张 IPAV 说明毒力变细。 | Yi Zhong Xiao Chang Xing-Jun Cao Yan Zhang Huajun Zheng Yongzhang Zhu Chengsong Cai Zelin Cui Yunyi Zhang Yuan-Yuan Li Xiu-Gao Jiang Guo-Ping Zhao Shengyue Wang Yixue Li Rong Zeng Xuan Li Xiao-Kui Guo | 2011 | Cell Research2011，21，8： | 10 |
| 12 | Risk analysis for the highly pathogenic avian influenza in China's Mainland using meta-modeling显示文摘A logistic model was employed to correlate the outbreak of highly pathogenic avian influenza (HPAI) with related environmental factors and the migration of birds. Based on MODIS data of the normalized difference vegetation index, environmental factors were considered in generating a probability map with the aid of logistic regression. A Bayesian maximum entropy model was employed to explore the spatial and temporal correlations of HPAI incidence. The results show that proximity to water bodies and national highways was statistically relevant to the occurrence of HPAI. Migratory birds, mainly waterfowl, were important infection sources in HPAI transmission. In addition, the HPAI outbreaks had high spatiotemporal autocorrelation. This epidemic spatial range fluctuated 45 km owing to different distribution patterns of cities and water bodies. Furthermore, two outbreaks were likely to occur with a period of 22 d. The potential risk of occurrence of HPAI in China's Mainland for the period from January 23 to February 17, 2004 was simulated based on these findings, providing a useful meta-model framework for the application of environmental factors in the prediction of HPAI risk. | CAO ChunXiang XU Min CHANG ChaoYi XUE Yong ZHONG ShaoBo FANG LiQun CAO WuChun ZHANG Hao GAO MengXu HE QiSheng ZHAO Jian CHEN Wei ZHENG Sheng LI XiaoWen | 2010 | Chinese Science Bulletin2010，55，36： | 9 |
| 13 | Study on biomass catalytic pyrolysis for production of bio-gasoline by on-line FTIR显示文摘The pyrolysis of biomass is a promising way for production of bio-gasoline if the stability and quality problems of the bio-crude-oil can be solved by catalytic cracking and reforming.In this paper,an on-line infrared spectrum was used to study thecharacteristics of catalytic pyrolysis with the following preliminary results.The removal of C O of organic acid is more difficultthan that of aldehydes and ketones.HUSY/γ-Al_2O_3 and REY/γ-Al_2O_3 catalysts exhibited better deoxygenating activities whileHZSM-5/γ-Al_2O_3 catalyst exhibited preferred selectivities for production of iso-alkanes and aromatics.Finally,possiblemechanisms of biomass catalytic pyrolysis are discussed as well. | Chang Bo Lu Jian Zhong Yao Wei Gang Lin Wen Li Song | 2007 | Chinese Chemical Letters2007，18，4： | 9 |
| 14 | Pollen Typhae Total Flavone Inhibits Endoplasmic Reticulum Stress-Induced Apoptosis in Human Aortic-Vascular Smooth Muscle Cells through Down-Regulating PERK-eIF2α-ATF4-CHOP Pathway显示文摘Objective: To test the hypothesis that the inhibition of endoplasmic reticulum(ER) stress-induced apoptosis in oxidized low-density lipoproteins(ox-LDL)-induced human aortic-vascular smooth muscle cells(HA-VSMCs) was associated with suppression of the protein kinase RNA-like ER kinase(PERK)-eukaryotic translation initiation factor 2α(e IF2α)-activating transcription factor 4(ATF4)-CCAAT/enhancer binding protein homologous protein(CHOP) signaling pathway by Pollen Typhae total flavone(PTF). Methods: Primary HA-VSMCs were cultured and identified. The cultured HA-VSMCs were randomized into 5 groups, including a normal control group, an ox-LDL group(70 μg/m L high ox-LDL), an HPTF group(70 μg/m L high ox-LDL+500 μg/m L PTF), an MPTF group(70 μg/m L high ox-LDL+250 μg/m L PTF), and a LPTF group(70 μg/m L high ox-LDL+100 μg/m L PTF) in the first part;and a normal control group, an ox-LDL group(70 μg/mL high ox-LDL), an MPTF group(70 μg/m L high ox-LDL+250 μg/m L PTF), a sh RNA group(transducted with PERK shRNA lentiviral particles), a scramble shRNA group(transducted with control shRNA lentiviral particles), an MPTF+ox-LDL+shRNA group(250 μg/mL PTF+70 μg/mL high ox-LDL+PERK shRNA lentiviral particles) and an ox-LDL+shRNA group(70 μg/mL high ox-LDL+PERK shRNA lentiviral particles) in the second part. The protein expression levels of ER-associated apoptosis proteins were detected by Western blot, and their m RNA expression levels were detected by quantitative real-time reverse transcription-polymerase chain reaction. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay was applied to test cel viability, and the level of apoptosis was monitored by flow cytometry. Results: The MTT assay and flow cytometry showed that the ox-LDL group had a significant increase in apoptosis, which was attenuated in PTF treatment groups and sh RNA groups. Moreover, the ox-LDL group had increased protein and m RNA levels of binding immunoglobulin protein and ER-associated apoptosis proteins, such as PERK, e IF2α, ATF4 and CHOP, which were attenuated in PTF treatment groups and sh RNA groups. Conclusions: The apoptosis induced by ox-LDL had a strong relation to ER stress. The protective effect of PTF on ER stressinduced apoptosis was associated with inhibition of the PERK-eIF2α-ATF4-CHOP pathway, which might be a potential therapeutic strategy for enhancing the stability of atherosclerotic plaques. | CHEN Ming-tai HUANG Ruo-lan OU Li-jun CHEN Ying-nan MEN Ling CHANG Xiao WANG Ling YANG Yu-zhu ZHANG Zhong | 2019 | Chinese Journal of Integrative Medicine2019，25，8： | 9 |
| 15 | Olivine-norite rock detected by the lunar rover Yutu-2 likely crystallized from the SPA-impact melt pool显示文摘Chang’E-4 landed in the South Pole-Aitken(SPA)basin,providing a unique chance to probe the composition of the lunar interior.Its landing site is located on ejecta strips in Von Karm´an crater that´possibly originate from the neighboring Finsen crater.A surface rock and the lunar regolith at 10 sites along the rover Yutu-2 track were measured by the onboard Visible and Near-Infrared Imaging Spectrometer in the first three lunar days of mission operations.In situ spectra of the regolith have peak band positions at 1 and 2μm,similar to the spectral data of Finsen ejecta from the Moon Mineralogy Mapper,which confirms that the regolith’s composition of the landing area is mostly similar to that of Finsen ejecta.The rock spectrum shows similar band peak positions,but stronger absorptions,suggesting relatively fresh exposure.The rock may consist of 38.1±5.4%low-Ca pyroxene,13.9±5.1%olivine and 48.0±3.1%plagioclase,referred to as olivine-norite.The plagioclase-abundant and olivine-poor modal composition of the rock is inconsistent with the origin of the mantle,but representative of the lunar lower crust.Alternatively,the rock crystallized from the impact-derived melt pool formed by the SPA-impact event via mixing the lunar crust and mantle materials.This scenario is consistent with fast-cooling thermal conditions of a shallow melt pool,indicated by the fine to medium-sized texture(<3 mm)of the rock and the SPA-impact melting model[Icarus 2012;220:730–43]. | Honglei Lin Zhiping He Wei Yang Yangting Lin Rui Xu Chi Zhang Meng-Hua Zhu Rui Chang Jinhai Zhang Chunlai Li Hongyu Lin Yang Liu Sheng Gou Yong Wei Sen Hu Changbin Xue Jianfeng Yang Jie Zhong Xiaohui Fu Weixing Wan Yongliao Zou | 2020 | National Science Review2020，7，5： | 9 |
| 16 | Origin and Evolution of Core Components Responsible for Monitoring Light Environment Changes during Plant Terrestrialization显示文摘Light serves as the source of energy as well as an information signal for photosynthetic plants. During evolution, plants have acquired the ability to monitor environmental light radiation and adjust their developmental patterns to optimally utilize light energy for photosynthesis. The mechanisms of light perception and signal transduction have been comprehensively studied in past decades, mostly in a few model plants, including Arabidopsis thaliana. However, systematic analyses of the origin and evolution of core components involved in light perception and signaling are still lacking. In this study, we took advantage of the recently sequenced genomes and transcriptomes covering all the main Archaeplastida clades in the public domain to identify orthologous genes of core components involved in light perception and signaling and to reconstruct their evolutionary history. Our analyses suggested that acclimation to different distribution of light quality in new environments led to the origination of specific light signaling pathways in plants. The UVR8 (UV Resistance Locus 8) signaling pathway originated during the movement of plants from the deeper sea to shallow water and enabled plants to deal with ultraviolet B light (UV-B). After acquisition of UV-B adaptation, origination of the phytochrome signaling pathway helped plants to colonize water surface where red light became the prominent light energy source. The seedling emergence pathway, which is mediated by a combination of light and phytohormone signals that orchestrate plant growth pattern transitions, originated before the emergence of seed plants. Although cryptochromes and some key components of E3 ubiquitin ligase systems already existed before the divergence of the plant and animal kingdoms, the coevolution and optimization of light perception and downstream signal transduction components, including key transcription factors and E3 ubiquitin ligase systems, are evident during plant terrestrialization. | Xue Han Xin Chang Zhenhua Zhang Haodong Chen Hang He Bojian Zhong Xing Wang Deng | 2019 | Molecular Plant2019，12，6： | 9 |
| 17 | Rapid Detection of Haemophilus influenzae and Haemophilus parainfluenzae in Nasopharyngeal Swabs by Multiplex PCR显示文摘Objective To establish multiplex PCR-based assays for detecting H.influenzae and H.parainfluenzae.And the PCR-based assays were applied to detect the carriage rates of H.influenzae and H.parainfluenzae in nasopharyngeal swab specimens which were collected from healthy children.Methods Multiplex primers for species-specific PCR were designed by using DNAstar soft based on the sequences of 16S rRNA genes from genus Haemophilus to detect H.influenzae and H.parainfluenzae.Results The sensitivity of the 16S rRNA PCR assay for detecting H.influenzae and H.parainfluenzae was 97.53% and 100% respectively,and the specificity was 95.89% and 96.63% respectively.Youden's Index on the ability to detect H.influenzae and H.parainfluenzae was 0.9342 and 0.9663 respectively.666 nasopharyngeal swab specimens were collected from healthy children.The detection rates of H.influenzae and H.parainfluenzae were 14.11% and 16.07% respectively by using isolation and culture methods.The detection rates of H.influenzae and H.parainfluenzae were 43.54% and 57.96% respectively by 16S rRNA PCR assays.The carriage rates of serotypes a,b,c,d,e,f and non-typeable isolates were 0% (0/666),0.15% (1/666),1.20% (8/666),0.15% (1/666),1.20% (8/666),1.80% (12/666),95.50% (636/666) respectively.Conclusion The multiplex PCR assays were very rapid,reliable and feasible methods for detection of H.influenzae and H.parainfluenzae in pharyngeal swab specimens which were compared to conventional isolation and culture methods.95.5% of H.influenzae strains in healthy children were nontypeable.The encapsulated or typable strains were mainly three serotypes which was c,e,and f serotype. | TIAN Guo Zhong ZHANG Li Juan WANG Xiao Lei ZHANG Li LI Shu Feng GU Chang Mei SUN Jian CUI Bu Yun | 2012 | Biomedical and Environmental Sciences2012，25，3： | 8 |
| 18 | Research advances on the usage of traditional Chinese medicine for neuroprotection in glaucoma显示文摘Progressive loss of retinal ganglion cells (RGCs) and their axons is the main pathogenesis of glaucoma. The cause of glaucoma is not fully understood, but the neurodegeneration of glaucoma involves many mechanisms such as oxidative stress, glutamate toxicity and ischemia/ reperfusion insult. In order to target these mechanisms, multiple neuroprotective interventions have been investigated to prevent the death of RGCs. Of note are some tonic herbs from the traditional Chinese medicine (TCM) pharmacopeia that have shown neuroprotective effects in glaucoma. TCM differs from Western medicine in that TCM exhibits complicated bioactive components, triggering many signaling pathways and extensive actions on vital organs. Modern scientific approaches have demonstrated some of their underlying mechanisms. In this review, we used Lycium barbarum and Ginkgo biloba as examples to elaborate the characteristics of TCM and their potential applications in neuroprotection in glaucoma. | Xue-song Mi Jing-xiang Zhong Raymond Chuen-Chung Chang Kwok-Fai So | 2013 | Journal of Integrative Medicine2013，11，4： | 8 |
| 19 | APPLICATION OF GENETIC DEAFNESS GENE CHIP FOR DETECTION OF GENE MUTATION OF DEAFNESS IN PREGNANT WOMEN显示文摘Objective The study is to identify the carrier rate of common deafness mutation in Chinese pregnant women via detecting deafness gene mutations with gene chip.Methods The pregnant women in obstetric clinic without hearing impairment and hearing disorders family history were selected.The informed consent was signed.Peripheral blood was taken to extract genomic DNA.Application of genetic deafness gene chip for detecting 9 mutational hot spot of the most common 4 Chinese deafness genes,namely GJB2(35delG,176del16bp,235delC,299delAT),GJB3(C538T),SLC26A4(IVS72A>G,A2168G)and mitochondrial DNA 12S rRNA(A1555G,C1494T).Further genetic testing were provided to the spouses and newborns of the screened carriers.Results Peripheral blood of 430 pregnant women were detected,detection of deafness gene mutation carriers in 24 cases(4.2%),including 13 cases of the GJB2 heterozygous mutation,3 cases of SLC26A4 heterozygous mutation,1cases of GJB3 heterozygous mutation,and 1 case of mitochondrial 12S rRNA mutation.18 spouses and 17 newborns took further genetic tests,and 6 newborns inherited the mutation from their mother.Conclusion The common deafness genes mutation has a high carrier rate in pregnant women group,235delC and IVS7-2A>G heterozygous mutations are common. | CHANG Liang ZHONG Su ZHAO Nan LIU Ping ZHAO Yangyu QIAO Jie | 2014 | Journal of Otology2014，9，2： | 8 |
| 20 | Rapid Growth of Halide Perovskite Single Crystals:From Methods to Optimization Control显示文摘Metal halide perovskites are emerging as new generation optoelectronic materials due to their high carrier mobility, long carrier diffusion length and large light absorption coefficient, which have broad applications in solar cell, light-emitting diode, laser, photodetector and transistors. Perovskite single crystal is an ideal platform for discerning the intrinsic properties of these materials. In some cases, perovskite single crystals are better candidates to gain high performance optoelectronics. However, the growth of perovskite single crystals is time and cost consuming, which has an obvious disadvantage for device exploration. Therefore, fast growth technique is highly desirable in not only promoting the use of perovskites in commercial applications but also facilitating deep physical investigation of the materials. In this review, we summarize thoroughly the development of fast growth of the halide perovskites single crystal. Specifically, we highlight the progress of rapid growth techniques with emphasis on the optimization control. | Yu-Ling Wang Shuai Chang Xiao-Mei Chen Yan-Dong Ren Li-Fu Shi Yong-Hao Liu Hai-Zheng Zhong | 2019 | Chinese Journal of Chemistry2019，37，6： | 8 |
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